WO1991016977A1 - Technique de manipulation de liquide a l'aide d'une pipette - Google Patents

Technique de manipulation de liquide a l'aide d'une pipette Download PDF

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Publication number
WO1991016977A1
WO1991016977A1 PCT/FI1991/000136 FI9100136W WO9116977A1 WO 1991016977 A1 WO1991016977 A1 WO 1991016977A1 FI 9100136 W FI9100136 W FI 9100136W WO 9116977 A1 WO9116977 A1 WO 9116977A1
Authority
WO
WIPO (PCT)
Prior art keywords
liquid
volume
liquid volume
transferred
pipette
Prior art date
Application number
PCT/FI1991/000136
Other languages
English (en)
Inventor
Osmo Suovaniemi
Pertti Ekholm
Original Assignee
Biohit Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biohit Oy filed Critical Biohit Oy
Publication of WO1991016977A1 publication Critical patent/WO1991016977A1/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0227—Details of motor drive means

Definitions

  • the present invention concerns a procedure, as defined in the preamble to claim 1, for liquid handling with the aid of a pipette.
  • Liquid handling is in this context understood to mean accurate and reproducible transfer, dilution, dispensing, mixing, titration and other manipulation of a desired quantity of liquid.
  • the pipette may be a device with one passage or with multiple passages. In a ⁇ multi-passage device is for instance provided a plural ⁇ ity of side-by-side, identical devices, which most ad ⁇ vantageously can be made to operate simultaneously.
  • a pipette which comprises a plunger, a liquid volume and a plunger operating means for transferring a desired quantity of liquid into, and out from, the liquid volume.
  • the operating means usually includes an electric motor with the aid of which the transfer is implemented.
  • the problem in liquid handling carried out with a pipette is accuracy, speed and reproducibility and, in particular, fitting these together.
  • the liquid quantity to be handled e.g. in dispensing, is usually small, but it should be handled with a given accuracy and in reproducible manner.
  • the han ⁇ dling of the liquid quantity should be fast in order that the means should be able to process a great number e.g. of samples.
  • the object of this invention is to eliminate the drawbacks cited in the foregoing.
  • the procedure of the invention is character ⁇ ized in that in a first step liquid is transferred into the liquid volume of the pipette with the aid of a plunger from a given starting point in this volume by moving the plunger with a substantially linearly accel ⁇ erated velocity, until the desired, predetermined con ⁇ stant speed of plunger and liquid has been reached; in a second step the plunger and the liquid are moved at constant speed; in a third step the liquid transfer is abruptly stopped when the liquid quantity that is to be transferred has been loaded into the liquid volume; and the liquid quantity is dispensed in one or several batches out from the liquid volume in accordance with the respective steps just described, so that the liquid transfer steps constitute a closed cycle.
  • decelera ⁇ tion of the plunger movement and of the liquid transfer is commenced with substantially linearly decelerated speed from said constant speed when the liquid quantity already transferred is observed to be close to the ul ⁇ timate liquid quantity to be transferred.
  • all veloci- ties or speeds, accelerations and decelerations can be set in advance.
  • all veloci ⁇ ties or speeds, accelerations and decelerations can be selected in advance from a certain, predetermined set of velocity, speed, acceleration and deceleration val ⁇ ues.
  • the last step is a secondary phase in which the liquid volume is emptied past the fixed starting point where the plunger was located at the beginning of the first step.
  • the liquid volume is emptied by moving the plunger as far as a lower limit, whereafter the liquid volume is returned to the initial state of the first step by moving the plunger to the starting point.
  • At dispens ⁇ ing the liquid quantity out from the liquid volume one goes from the step of constant-speed plunger and liquid movement to a substantially linear deceleration step, which is replaced at the beginning of the secondary phase with a substantially linear acceleration step, and further with a constant speed step, from which one goes, most advantageously, to a substantially linear deceleration step, until the lower limit of the liquid volume is reached, whereafter the plunger is moved from the lower limit of the liquid volume, most advantage ⁇ ously through equivalent steps to the starting point, and the liquid volume is returned to the initial state of the first step, where the process is stopped.
  • An advantage of the procedure of the invention is exactitude and accuracy of liquid dispensing.
  • a further advantage of the procedure of the invention is the ease of its modification and its flex ⁇ ibility, whereby it is possible to adapt this procedure to different pipettes, and to single passage as well as multiple passage pipettes.
  • a further advantage of the procedure of the invention is the versatility of its use in conjunction with any suitable single or multiple passage pipette. Any handling of liquid quantities is managed with ease, whatever the application.
  • a further advantage of the procedure of the invention is that termination both of the intake phase and of the dispensing phase is implemented abruptly, by arresting the plunger movement e.g. with the aid of an efficient brake means, whereby no residual liquid drop ⁇ lets are incurred on the tip portion of the pipette. Emptying of the liquid volume, in particular, will therefore take place sharply and accurately, and in ⁇ accuracy due to droplets can thus be avoided.
  • Fig. 1 presents, schematically, a pipette
  • Fig. 2 presents the kinetic diagram of the pipette plunger and of the liquid transfer, in general form
  • Fig. 3 illustrates the basic operation of the pipette with the aid of a kinetic diagram
  • Fig. 4 illustrates a dispensing operation implemented with the aid of a pipette, by the aid of a kinetic dia ⁇ gram
  • Fig. 5 illustrates a diluting operation implemented with the aid of a pipette, by the aid of a kinetic dia ⁇ gram.
  • Fig. 1 is schematically depicted a pipette comprising a cylinder or liquid volume 1; a plunger 2, fitted into the cylinder volume; a tip part 3; a liquid passage 4 to connect the liquid volume 1 with the tip part; an operating means 5 for moving the plunger 2 in the liquid volume; and a control means 6 for control ⁇ ling the operating means 5 and by its mediation, the movements of the plunger 2.
  • Fig. 2 is depicted the kinetic diagram of the plunger and liquid movement, the x axis repre ⁇ senting the longitudinal direction A-A of the liquid volume, and at the same time of the pipette, and where the length of the liquid volume is 1 .
  • On the y axis of the kinetic diagram is reproduced the velocity with which the plunger is moved, and at the same time the liquid movement speed. It is thus understood that the upper limit and lower limit of the movement of the plunger 2 are separated by the distance 1 .
  • the procedure of the invention is generally described, referring to this kinetic diagram. Liquid is being transferred into the liquid volume 1 of the pipette.
  • liquid is transferred into the liquid volume 1 of the pipette with the aid of the operating means 5 and plunger ⁇ , under control by the control means 6, from a fixed starting point a in this volume by moving the plunger with substantially linearly accelerated velocity Al, until the desired, predetermined constant speed Bl of plunger and liquid has been reached.
  • the plunger 2 and the liquid are moved at constant speed B10,B11.
  • the liquid transfer is abruptly stopped when the liquid quantity to be transferred has been loaded into the liquid volume Cl.
  • the liquid quantity is dispensed from the liquid volume in one or several batches by respective steps like those just described, so that the liquid transfer steps constitute a closed cycle.
  • the whole operation is presented step by step in Fig. 2.
  • All velocities, speeds, accelerations and de ⁇ celerations Al, Bl, BIO, Bll, Dl can be preset. These velocities, speeds, accelerations and decelerations are advantageously selectable in advance from a certain set of velocity, speed, acceleration and deceleration val ⁇ ues, which can be entered in the memory of the control means.
  • Figs 2 and 3 reveal, in the operation of dispensing the liquid quantity from the liquid volume in one (Fig. 2) or several batches (Fig. 3; H, 1-6, W) the last step is a secondary phase T, in which the li- quid volume is emptied past the fixed starting point a of the plunger 2, the location of the plunger at the beginning of the first step.
  • the liquid quantity when the liquid quantity is dispensed out from the liquid vol ⁇ ume, one goes from the step of plunger 2 and liquid movement at constant speed Bl to a substantially linear deceleration step Dl, which is replaced, at the begin ⁇ ning of the secondary step T, with a substantially lin- ear acceleration step Tl and, further, with a constant speed step T2, from which one goes most advantageously to a substantially linear deceleration step T3, until the lower limit ar of the liquid volume is reached.
  • the plunger 2 is moved from the lower limit ar of the liquid volume 1, through corresponding steps T3,T4, to the starting point a .
  • the liquid volume has been re ⁇ turned to the initial state of the first step, and the process is stopped there. In this case, too, the move ⁇ ment of the plunger 2 back to the starting point is arrested abruptly, T5.
  • Fig. 5 illustrates, with the aid of a kinetic diagram, a diluting operation implemented with the aid of a pipette.
  • reagent is transferred into the liquid volume with the aid of the plunger 2 in an inspiration phase Rl, starting at the starting posi ⁇ tion a , whereafter in a second step I an air bubble is drawn into the liquid volume 1, and thereafter fol ⁇ lows the sample inspiration R2.
  • Dispensing takes place similarly as shown in Fig. 3. On the dispensing follows a secondary phase T, as has already been described.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

Technique de manipulation de liquide à l'aide d'une pipette dans laquelle du liquide est transféré dans le volume de liquide de la pipette à partir d'un certain point de départ de ce volume, à une vitesse (A1) accélérée de manière linéaire, jusqu'à ce qu'une vitesse (B1) prédéterminée voulue constante ait été atteinte. Le liquide est transféré à une vitesse constante (B1). Le transfert de liquide est stoppé sans transition (D1) lorsque la quantité de liquide à transférer a été chargée dans le volume de liquide, et la quantité de liquide est distribuée à partir du volume de liquide en un ou en plusieurs lots selon les étapes respectives (A, B, D) présentées au préalable, de manière que les étapes de transfert de liquide constituent un cycle fermé.
PCT/FI1991/000136 1990-05-04 1991-05-03 Technique de manipulation de liquide a l'aide d'une pipette WO1991016977A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI902271A FI84790B (fi) 1990-05-04 1990-05-04 Foerfarande foer behandling av vaetska medelst pipett.
FI902271 1990-05-04

Publications (1)

Publication Number Publication Date
WO1991016977A1 true WO1991016977A1 (fr) 1991-11-14

Family

ID=8530390

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FI1991/000136 WO1991016977A1 (fr) 1990-05-04 1991-05-03 Technique de manipulation de liquide a l'aide d'une pipette

Country Status (2)

Country Link
FI (1) FI84790B (fr)
WO (1) WO1991016977A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2714181A1 (fr) * 1993-12-22 1995-06-23 Peters Jean Bernard Système automatique de répartition d'un liquide biologique.
US5985214A (en) * 1997-05-16 1999-11-16 Aurora Biosciences Corporation Systems and methods for rapidly identifying useful chemicals in liquid samples
EP1437181A2 (fr) * 2001-10-17 2004-07-14 MUSASHI ENGINEERING, Inc. Procede de distribution de materiau liquide et dispositif associe
WO2005009630A1 (fr) 2003-07-25 2005-02-03 Musashi Engineering, Inc. Procede de regulation d'une distribution de gouttes de liquide, procede de distribution de gouttes de liquide et dispositif de distribution de gouttes de liquide

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE385090B (sv) * 1971-06-23 1976-06-08 Suovaniemi Osmo Antero Pipett

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE385090B (sv) * 1971-06-23 1976-06-08 Suovaniemi Osmo Antero Pipett

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2714181A1 (fr) * 1993-12-22 1995-06-23 Peters Jean Bernard Système automatique de répartition d'un liquide biologique.
US6685884B2 (en) 1997-05-16 2004-02-03 Vertex Pharmaceuticals, Inc. Methods for rapidly identifying useful chemicals in liquid sample
US7105132B2 (en) 1997-05-16 2006-09-12 Aurora Discovery, Inc. Liquid chemical distribution method and apparatus
US6468800B1 (en) 1997-05-16 2002-10-22 Vertex Pharmaceuticals (San Diego), Llc Systems and methods for rapidly identifying useful chemicals in liquid samples
US6472218B1 (en) 1997-05-16 2002-10-29 Vertex Pharmaceuticals (San Diego), Llc Systems and methods for rapidly identifying useful chemicals in liquid samples
US6678577B1 (en) 1997-05-16 2004-01-13 Vertex Pharmaceuticals Incorporated Systems and methods for rapidly identifying useful chemicals in liquid samples
US5985214A (en) * 1997-05-16 1999-11-16 Aurora Biosciences Corporation Systems and methods for rapidly identifying useful chemicals in liquid samples
US6372185B1 (en) 1997-05-16 2002-04-16 Aurora Biosciences Corporation Liquid chemical distribution method and apparatus
US6890485B1 (en) 1997-05-16 2005-05-10 Aurora Discovery, Inc. High throughput chemical handling system
EP1437181A2 (fr) * 2001-10-17 2004-07-14 MUSASHI ENGINEERING, Inc. Procede de distribution de materiau liquide et dispositif associe
EP1437181B1 (fr) * 2001-10-17 2012-05-09 Musashi Engineering, Inc. Procede de distribution de materiau liquide et dispositif associe
WO2005009630A1 (fr) 2003-07-25 2005-02-03 Musashi Engineering, Inc. Procede de regulation d'une distribution de gouttes de liquide, procede de distribution de gouttes de liquide et dispositif de distribution de gouttes de liquide
EP1649938A1 (fr) * 2003-07-25 2006-04-26 MUSASHI ENGINEERING, Inc. Procede de regulation d'une distribution de gouttes de liquide, procede de distribution de gouttes de liquide et dispositif de distribution de gouttes de liquide
EP1649938A4 (fr) * 2003-07-25 2008-10-01 Musashi Engineering Inc Procede de regulation d'une distribution de gouttes de liquide, procede de distribution de gouttes de liquide et dispositif de distribution de gouttes de liquide
US7645018B2 (en) 2003-07-25 2010-01-12 Musashi Engineering, Inc. Method of adjusting a liquid droplet, method of discharging the liquid droplet and apparatus therefor

Also Published As

Publication number Publication date
FI902271A (fi) 1991-10-15
FI902271A0 (fi) 1990-05-04
FI84790B (fi) 1991-10-15

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