WO1991014706A3 - Lectin affinity membranes and methods for using them - Google Patents
Lectin affinity membranes and methods for using them Download PDFInfo
- Publication number
- WO1991014706A3 WO1991014706A3 PCT/US1991/001578 US9101578W WO9114706A3 WO 1991014706 A3 WO1991014706 A3 WO 1991014706A3 US 9101578 W US9101578 W US 9101578W WO 9114706 A3 WO9114706 A3 WO 9114706A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- methods
- glycosylated proteins
- affinity
- glycosylated
- lectin affinity
- Prior art date
Links
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D67/00—Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
- B01D67/0081—After-treatment of organic or inorganic membranes
- B01D67/0093—Chemical modification
- B01D67/00931—Chemical modification by introduction of specific groups after membrane formation, e.g. by grafting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D67/00—Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
- B01D67/0081—After-treatment of organic or inorganic membranes
- B01D67/0093—Chemical modification
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
- B01D69/14—Dynamic membranes
- B01D69/141—Heterogeneous membranes, e.g. containing dispersed material; Mixed matrix membranes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28014—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their form
- B01J20/28033—Membrane, sheet, cloth, pad, lamellar or mat
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/53—Colony-stimulating factor [CSF]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
Abstract
This invention relates to lectin affinity membranes and methods for carrying out affinity separations of glycosylated proteins using them. The method utilizes a membrane-bound lectin ligand first to capture and separate a glycosylated protein from a fluid mixture and subsequently to release said protein in purified form. The present affinity membrane methods recover high-purity glycosylated proteins. The invention has particular relevance to the recovery and purification of glycosylated proteins from mixtures of biological origin.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US49546890A | 1990-03-19 | 1990-03-19 | |
US495,468 | 1990-03-19 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO1991014706A2 WO1991014706A2 (en) | 1991-10-03 |
WO1991014706A3 true WO1991014706A3 (en) | 1991-11-14 |
Family
ID=23968753
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1991/001578 WO1991014706A2 (en) | 1990-03-19 | 1991-03-08 | Lectin affinity membranes and methods for using them |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO1991014706A2 (en) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2275270A (en) * | 1993-02-11 | 1994-08-24 | Pall Corp | Membranes for use in affinity separation |
WO2004064608A2 (en) | 2003-01-17 | 2004-08-05 | Aethlon Medical, Inc. | Method for removal of viruses from blood by lectin affinity hemodialysis |
WO2004090549A1 (en) * | 2003-04-11 | 2004-10-21 | Novozymes A/S | Method of screening for secreted glycosylated polypeptides |
DE102005001191A1 (en) * | 2005-01-10 | 2006-07-20 | Qiagen Gmbh | Apparatus and method for fractionating mono- and / or oligosaccharide structures |
US20070037933A1 (en) * | 2005-08-11 | 2007-02-15 | Kurth Christopher J | Surface modified polymer matrices and methods for their preparation |
CN111902720A (en) | 2018-03-21 | 2020-11-06 | 沃特世科技公司 | Non-antibody high affinity based sample preparation, adsorbents, devices and methods |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DD159569A1 (en) * | 1981-06-05 | 1983-03-16 | Manfred Kuehn | Test agent and method for the determination of glycosylated proteins |
WO1990004609A1 (en) * | 1988-10-17 | 1990-05-03 | Sepracor, Inc. | Process for the covalent surface modification of hydrophobic polymers and articles made therefrom |
-
1991
- 1991-03-08 WO PCT/US1991/001578 patent/WO1991014706A2/en unknown
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DD159569A1 (en) * | 1981-06-05 | 1983-03-16 | Manfred Kuehn | Test agent and method for the determination of glycosylated proteins |
WO1990004609A1 (en) * | 1988-10-17 | 1990-05-03 | Sepracor, Inc. | Process for the covalent surface modification of hydrophobic polymers and articles made therefrom |
Non-Patent Citations (1)
Title |
---|
Chemical Abstracts, Volume 110, No. 25, issued 1989, June 19 (Columbus, Ohio, USA),M.G. SCHER et al. "Stabilization of immobilized lectin columns by cross- linking with glutaraldelujde ", see pages 299-300, right and left columns, the abstract-No. 227 913p, Anal. Biochem. 1989,177(1), 168-71. * |
Also Published As
Publication number | Publication date |
---|---|
WO1991014706A2 (en) | 1991-10-03 |
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