WO1991012720A1 - Method of sterilizing plant pollen - Google Patents

Method of sterilizing plant pollen Download PDF

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Publication number
WO1991012720A1
WO1991012720A1 PCT/SU1990/000048 SU9000048W WO9112720A1 WO 1991012720 A1 WO1991012720 A1 WO 1991012720A1 SU 9000048 W SU9000048 W SU 9000048W WO 9112720 A1 WO9112720 A1 WO 9112720A1
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Prior art keywords
sterilizing
agenτοm
chτο
sποsοb
shesτοgο
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PCT/SU1990/000048
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French (fr)
Russian (ru)
Inventor
Marat Alexandrovich Fedin
Tatyana Alexandrovna Kuznetsova
Sergei Ivanovich Paklin
Vladimir Mikhailovich Fedoseev
Yaroslav Ivanovich Lys
Svetlana Vladimirovna Volkova
Nina Nikolaevna Troshina
Tatyana Petrovna Trofimova
Mikhail Nikolaevich Semenenko
Valentin Anatolievich Savchuk
Sergei Ivanovich Orlov
Georgy Sergeevich Muromtsev
Olga Dmitrievna Tsiunovich
Svetlana Alexandrovna Novikova
Original Assignee
Vsesojuzny Nauchno-Issledovatelsky Institut Selskokhozyaistvennoi Biotekhnologii
Moskovsky Gosudarstvenny Universitet Imeni M.V.Lomonosova
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Priority to PCT/SU1990/000048 priority Critical patent/WO1991012720A1/en
Publication of WO1991012720A1 publication Critical patent/WO1991012720A1/en

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/40Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
    • A01N47/42Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/74Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
    • A01N43/781,3-Thiazoles; Hydrogenated 1,3-thiazoles
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/84Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms six-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,4

Definitions

  • the most advantageous products are those based on the sterilization of dust by chemical sterilizing agents (gametes).
  • gametes chemical sterilizing agents
  • the use of gametes is more economical than the use of
  • sterilizing agents such as 2-hydroxyethyl acid (etheral), maleic acid hydride, di- (polysulphuric acid). Processing of products with sterilizing agents is carried out by U or U at this stage of genesis ( ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ⁇ ).
  • the ossevsky invention posed a task by means of the method of sterilizing the user with the help of the operating system. - h - use it for sterilization of dusts of a wide circle of culture with a high erysionic risk of being connected to a high temperature.
  • the solution to the problem is that, in the proposed way, the dust is discharged by the process, and the solution is disregarded,
  • ⁇ 2 C 3 ⁇ ? ; ⁇ 2 ⁇ ; ⁇ ⁇ ⁇ ⁇ ( ⁇ ) ⁇ ⁇ ⁇ ; ⁇ ;
  • Sterilizing agents may be used in combination with any pestilent discharger. Use it in conjunction with the water in the form of the 2 ⁇ -th
  • the proposed method makes it possible to grow a masculine plant growth (71.3-100.0) and to keep a high rate of seed binding (above 78.8%). In order to ensure a high level of ⁇ -sterilization of dust particles in case of unfavorable climatic conditions, it is beneficial to the use of non-hazardous substances.
  • ⁇ 2 ⁇ 3 n ⁇ ; ⁇ n 2 vg; ⁇ terrorism 2 ⁇ ( ⁇ ) ⁇ ntended 2 .orggina ⁇ ; ⁇ ;
  • LD 50 is a 2-amide 20-5-nitrate-2-thiazolene for white mice, which is 61 mg / kg live weight
  • LD ⁇ di-diomethyl-amyl-2-enylmene urea for white mice is 41 mg / kg
  • -5- ⁇ yl-2-thiazoline for white ⁇ mice is. 5 380 mg / kg of body weight and LD 5 ⁇ 2-amino-4-oxo-5- ⁇ - ⁇ -2-shiesolina for krays make 315 mg / kg of live weight.
  • the occurrence of the stages of genogenesis in the cortex is 5 logical.
  • the plant treatment plant with a sterilizing agent is at the beginning of the fifth stage of organogenesis (human plant).
  • the emulsion contains emulsifier - 0, 5 mes. C ⁇ -C- ⁇ malkylbenzene sulfonete and adjuvant - 0,01 mes dimethyl sulfoxide. ⁇ eskhod ⁇ Canal ⁇ 20 20 kg / gz. Counter-15.
  • the plants are plants that are treated with a disinfectant without a sterilizing generator.
  • Emulsion contains 0, 5 mes. C ⁇ - C ⁇ elkylbenzene sulfate-calcium and 0.01 mes-diethyl sulfide. Saving ⁇ 20 ⁇ ⁇ 20 20 makes 20 kg / g.
  • the emulsion contains 0.5 mes. ⁇ -C ⁇ malkylbenzene-sulfate-nethenia and 0.01 msec.% Dimethyl sulfide. ⁇ es ⁇ d
  • the claimed method is used in breeding and seed production for the production of highly productive agricultural crops and hybrids.

Abstract

In accordance with the claimed invention a method of sterilizing plant pollen provides for treating the plants, during the fifth and/or the sixth stage of organogenesis, with a sterilizing agent, chosen from a group comprising the derivatives of 2-thiazoline of general formula (I).HBr, where R¿1? = NH2; (a); (b); S - C 6H13; S - C8H17; S - C9H19; S - C10H21; R2 = C3H7; CH2Br; CH2SC(NH)NH2?.¿HBr; H; R¿3? + R4 = O; R3 = R4 = H.

Description

Сдοсοό сτеριшιзации πыльцы ρасτенлй Affordable pollen growth rate
Οбласτь τеχниκи Ηасτοящее язοбρеτение οτнοсиτся κ οбласτιз бøοлο- гии д сельсκοгο χοзяйсτва, а τοчнее κ сποсοбу сτеρили-The area of technology The prevailing language of the word is best for agriculture, and more precisely, for the sake of
5 заιдаи лыльниκοв ρасτений, яаχοдящему πρимеяеяие в се- леκции и семеяοвοдсτве .5 Go to the bastards of plants, which comes from the breeding and family.
Лρедшесτвувдий уροвень τеχниκи Β насτοящее вρемя в мπρе ρегаеτся задача инτенси- φиκация сельсκοгο χοзяйсτва , в часτнοсτи ποвышения уρο-The best level of technology Β the current time in the world, the task of intensifying the rural economy, in particular the increase in
Ю жайяοсτн зеρнοвыχ, κορмοвыχ, οвοщныχ и τеχяичесκяχ κу- льτуρ πуτем шиροκοгο исποльзοвания гябρидοв πеρвοгο πο- κοления. За счеτ явления ги бρидяοй силы эτи ги бρяды οτ- личаюτся οτ ροдиτельсκиχ φορм бοлее высοκαй лροдуκτнв- нοсτью (на 25-30/1) и высοκим κачесτвοм προдуκции . Суще-You can get grains, vegetables, vegetables, and vegetables by extensively using gabruids for food. Due to the appearance of gigabytes of strength, these gigabytes are not distinguished by higher product quality (25-30 / 1) and higher quality products. Essentially
15 сτвуеτ меτοд выведения нοвыχ гибρидοв, οснοванный на сисτеме "цøτοπлазмаτичесκая муасκая сτеρильнοсτь - вοс- сτанοвиτели φеρτильнοсτн". Эτοτ меτοд οснοван на длиτе- льнοй (в τечение 12-14 леτ) и слοжнοи селеκциοянοй ρа- бοτе, вκлючающей сοздание сτеρильяыχ аяалοгοв, заκρе-15 there is a method of introducing new hybrids, based on the system "solid-plastic mousse stability - vosstanoviteli actives". This method is based on a long (for 12-14 years) and complex breeding work, including the creation of sterile ayalugs, closed
20 яиτелей сτеρильяοсτн и вοссτанοвиτелей φеρτильяοсτи . Шибοлее πеρсπеκτявными являюτся сποсοοы, οснοванные на сτеρилизации πыльяиκοв ρасτеяий χимичесκими сτеρилизу- ющими агеяτами (гамеτοцидами ) . Исποльзοвание гамеτοцø- дοв οκазываеτся зяачиτельнο эκοнοмичнее , чем исποльзο-20 resident residents and foreigners of the economy. The most advantageous products are those based on the sterilization of dust by chemical sterilizing agents (gametes). The use of gametes is more economical than the use of
25 вание сисτемы "циτοπлазмаτичесκοй мужсκοй сτеρильяοсτн", τаκ κаκ яеτ неοбχοдимοсτи сοздаваτь τаκие φορмы, κаκ сτеρильный аяалοг, аяалοг заκρеπлеяия сτеρильнοсτи у маτеρинсниχ φορм и вοссτаяοвлеяия φеρτильнοсτя у οτцοв- сκиχ. Пρаκτичесκи мοжнο ποлучаτь семена гя бρидοв πеρвο-25 vanie sisτemy "tsiτοπlazmaτichesκοy muzhsκοy sτeρilyaοsτn" τaκ κaκ yaeτ neοbχοdimοsτi sοzdavaτ τaκie φορmy, κaκ sτeρilny ayaalοg, ayaalοg zaκρeπleyaiya sτeρilnοsτi in maτeρinsniχ φορm and vοssτayaοvleyaiya φeρτilnοsτya in οτtsοv- sκiχ. Practically you can irradiate seeds
30 гο ποκοлеяия κаκ в цροπессе селеκциοянοгο изучения ис- χοдныχ φορм, τаκ ζ πρи ορгаяизаιдаи иχ προмышленяοгο προизвοдсτва .30 gο ποκοleyaiya κaκ in tsροπesse seleκtsiοyanοgο study used χοdnyχ φορm, τaκ ζ πρi ορgayaizaιdai iχ προmyshlenyaοgο προizvοdsτva.
Κ насτοящему вρемеяи выявленο οκοлο 200 сοедияеяиζ, οбладающиχ гамеτοцидяοй аκτивнοсτью, οτяοсящиχся πο свοAt present, there have been identified about 200 compounds ζ possessing gametocidal activity, which remain for their own sake
35 ей χиыячесκοй сτρуκτуρе κ ρазличным κлассам χимичесκиχ сοедияений. Ιамеτοιдады дοлжны вызываτь πο вοзмοжнοсτг. ποляую мужсκую сτеρильнοсτь у οбρэ бοτаняκχ ρасτеяиϊ' πρв сοχρанеяиι. жøзнесποсοбнοсτΕ яицеκлеτοκ я οбесπечизаτь . - 2 - завязываемοсτь πρи свοбοднοм οπылеяии яа дοсτаτοчнο высοκοм уροвне ( желаτельяο, не менее 7( οτ κοяτροля) . Иχ φиτοτοκсичнοсτь и τοκсичнοсτь для τеπлοκροвныχ дοл-35 to her in a different structure for different classes of chemical compounds. Methods must be called up. I have a great masculine stability in the United States. Responsibility of the eggs I provide . - 2 - tied up with a conveniently high level of dust (if desired, at least 7 (excluded).
5 жны быτь мияимальяыми .5 wives be miyaymalyayami.
Извесτяы сποсοбы сτеρπлизацаπ πыльяиκοв зеρнοвыχ κульτуρ (Л.Дж.Ηиκелл. Ρегуляτορы ροсτа ρасτеяий. Пρи- менение в сельсκοм χοзяйсτве , Μοсκва , "Κοлοс", 1984, с . 8-31) , κοτορые заκлючаюτся в οбρабοτκе ρасτенийThe results of the disturbance of dust from the crops (L.J. Kikell. Regulators of the cultivate. Result in 1984, there is a waste of food).
10 сτеρилизущими агеяτами, τаκими κаκ 2-χлορэτилφοсφοяο- вая κислοτа (эτρел) , гидρазид малеинοвοй κислοτы, ди- (ποлиφτορалκил)-φοсφορяые κислοτы и иχ сοли и дρугие . Οбρабοτκу ρасτеяий сτеρилизующими агеяτами προвοдяτ яа У или УΙ эτаπе ορгаяοгенеза (πο Φ.Μ.Κуπеρмая) .10 sterilizing agents, such as 2-hydroxyethyl acid (etheral), maleic acid hydride, di- (polysulphuric acid). Processing of products with sterilizing agents is carried out by U or U at this stage of genesis (Φ Φ Φ Κ Κ π π π π π).
15 7 эτаπе ορганοгеяеза яачияаюτся προцессы οбρа- зοваяия и диφφеρеяцøацøи цвеτκοв. Β κοнце эτοгο эτаπа вοзяиκаюτ яοвοοбρазοваяия - сποροгенные аρχисπορеаль- яые τκаяи . Ба эτοм эτаπе идеτ заκладκа τычияοκ, πесτи- κа и ποκροвяыχ ορганοв цвеτκа. У эτаπе яа людаеτся15 7 the process of cultivation of flowers and the processes of flowers and differentiation of flowers. At the end of this phase, they develop jaundice, which are spongy aromatic real takayas. By this stage, the idea of laying a handy, end and end of the business of the flower. At this stage, I
20 яачалο дøφφеρенцøацøи τычинοчяοгο бугορκа яа τычияοч- яую яøτь и πесτиκ . УΙ эτаπ χаρаκτеρизуеτся προдессами φορмиροваяия цвеτκа (миκρο- и маκροсποροгеяез) . Ηа эτοм эτаπе οбычяο οбρазуюτся οбοсοблеяяые οдяοядеρяые πаль- πевые зеρяа (Φ.Μ.Κуπеρмая, "Μορφοφизиοлοгия ρасτеяий",20 the first year of the year, the first place is the same as the other side of the world. This stage is characterized by the flow of flowers (micro- and macrogeaea). In this way, the common, free-standing, red-and-white flesh (Μ. Μ.
25 Μοсκва , "Βысшя шκοла", 1973, с.30-36) .25 Russia, "Higher School", 1973, p.30-36).
Извесτен τаκже сποсοб сτеρилизации πыльяиκοв злаκο- выχ ρасτеяий ( αΒ, Α, Ι567Ι53) , κοτορый заκлючаеτся в τοм, чτο προвοдяτ οбρабοτκу злаκοвыχ ρасτеяий сτеρили- зующим агеяτοм в πеρиοд между ποявлеяием 2-го междοуз- 30 лия дο выκοлашиваяия. 3 κачесτве сτеρилизующегο агеяτа исποльзуюτ геτеροцøκлøчесκие сοедøяеяия, главными πρед- сτавиτелями κοτορыχ являюτся 2-κаρбοκси-3, -меτанοπиρρο- лидин илø 2-меτοκсиκаρбοнил-3, 4-меτанοπиρροлøдия. Уκа- заняые сοединения исποльзуюτся в сοчеτании с ρазοавиτе-Izvesτen τaκzhe sποsοb sτeρilizatsii πylyaiκοv zlaκοvyχ ρasτeyay (αΒ, Α, Ι567Ι53), κοτορy zaκlyuchaeτsya in τοm, chτο προvοdyaτ οbρabοτκu zlaκοvyχ ρasτeyay sτeρili- binder ageyaτοm in πeρiοd between ποyavleyaiem 2nd mezhdοuz- 30 lija dο vyκοlashivayaiya. 3 In the case of a sterilizing agent, they use hetero-electrical connectors, the main consumers of the batteries are 2-ports, they are 2-speed, they are 2-speed The indicated compounds are used in conjunction with
35 лями и ποвеρχяοсτнο-аκτивными вещесτвами . Гасκρыτие изοбρеτеяия Β οсяοву изοбρеτеяия ποлοжена задача πуτем ποдбορа сτеρилøзущиχ агеяτοв ρаз^йбοτаτь сποсοб, ποзвοляющий - з - исποльзοваτь егο для сτеρилизацøи πыльяиκοв шиροκοгο κρуга κульτуρ с высοκοй эφйеκτπвяοсτью сτеρилизацøи πρи сοχρаяеяии высοκοй завязываемοсτи семяя πρи свο- бοднοм οπылеяии.35 people and active-active substances. Plugging the Inventory The ossevsky invention posed a task by means of the method of sterilizing the user with the help of the operating system. - h - use it for sterilization of dusts of a wide circle of culture with a high erysionic risk of being connected to a high temperature.
Задача ρешеяа τем, чτο в πρедлагаемοм сποсοбе сτе- ρилизацøи πыльяиκοв ρасτеяий πуτем οбρа бοτκи иχ сτеρи- лизующим агеяτοм в сοчеτаяии с ρазбавиτелем в πеρиοд πяτοгο и/или шесτοгο эτаποв ορгаяοгенеза, сοгласяο изο-The solution to the problem is that, in the proposed way, the dust is discharged by the process, and the solution is disregarded,
10 бρеτеяию, в κачесτве сτеρилизующегο агенτа исποльзуωг προизвοдные 2-τиазοлияа οбщей φορмулы: т^ Й, н„ - σн - с10 brethren, in the quality of a sterilizing agent, a 2-type generalized formula 2 is used: m ^ J, n „- σn - s
Η,Η,
5 Ν5 Ν
\ / с Η Βг, н«\ / s Η Βг, н «
Figure imgf000005_0001
8 ~ С6Η13 ' 3 ~ σ8Η17 ' 3 ~ С9Η19 ' 8 "* '
Figure imgf000005_0001
8 ~ C 6 Η 13 ' 3 ~ σ 8 Η 17' 3 ~ C 9 Η 19 ' 8 "* '
Ε2 = С3Η? ; СΗ2Βг; СΗ^З С (Ш) ΝΗ^ΗΒг; Η ;
Figure imgf000005_0002
Ε 2 = C 3 Η ? ; СΗ 2 Βг; СΗ ^ З С (Ш) ΝΗ ^ ΗΒг; Η;
Figure imgf000005_0002
Сτеρилизувдий агеяτ мοжеτ быτь исποльзοвая в сοчеτаяии с любым язвесτяым πρигοдным ρазсавиτелем. Дэлесοοбρаз- нο исποльзοваτь егο з сοчеτаяии с вοдοй в виде 2^-яοйSterilizing agents may be used in combination with any pestilent discharger. Use it in conjunction with the water in the form of the 2 ^ -th
15 вοдяοй эмульсии . 3 κачесτве ρасτеяий, οбρа σаτываемыχ уκазаяяым сτеρилизующим агенτοм, πρедποчτяτельнο исποль- - - зуюτ злаκοвые ρасгения.15 water emulsions. 3 quality products, processed by a specified sterilizing agent, it is preferable to use - - Caused by malicious ragenia.
Пρедлэгаемый сποсοб ποзвοляеτ ποлучиτь мужсκую сτеρильнοсτь ρэсτений (71,3-100,0 ) и сοχρаниτь высο- κий προценг завязываемοсτи семян (выше 78,8%). С целью οбесπечения высοκοгο уροвня στеρилизации πыльниκοв πρи неблэгοπρияτныχ κлимаτичесκиχ услοвияχ προвοдяτ ποвгορную οбρабοτκу ρасτений сτеρилизующим агенτοм в πеρиοды πяτοгο иили шесτοгο эτаπа ορганοгенеза (ποThe proposed method makes it possible to grow a masculine plant growth (71.3-100.0) and to keep a high rate of seed binding (above 78.8%). In order to ensure a high level of σ-sterilization of dust particles in case of unfavorable climatic conditions, it is beneficial to the use of non-hazardous substances.
10 Κуπеρмэн).10 Kuyperman).
Лучший ваρиэнτ ρасκρыτия изοбρеτенияBEST VARIANT OF THE INVENTION
Заявляемый сποсοб οсущесτвления следующим οбρазοм.The inventive method of implementation as follows.
Ρэсτения, наπρимеρ, τаκие κаκ οзимая и'яροвая πшеница, диπлοидная ροжь οбρэбаτываюτ сгеρилизующим 5 агенτοм, в κзчесτве κοτοροгο исποльзуюτ προизвοдные 2-гиазοлина οбщей φορмулы:Ρesτeniya, naπρimeρ, τaκie κaκ οzimaya and 'yaροvaya πshenitsa, diπlοidnaya ροzh οbρebaτyvayuτ sgeρilizuyuschim 5 agenτοm in κzchesτve κοτοροgο isποlzuyuτ προizvοdnye 2-giazοlina οbschey φορmuly:
Figure imgf000006_0001
з я σ ΗΒг, где
Figure imgf000006_0001
s i σ ΗΒ g, where
Figure imgf000006_0002
κ2 = σ3нγ; σн2вг; σн2зσ(ιш)Бϊн2.нвг; Η;
Figure imgf000006_0002
κ 2 = σ 3 n γ ; σn 2 vg; σн 2 зσ (ιш) Бϊн 2 .нвг; Η;
Κ3 + Κ4 = 0; Ε3 = Κ^ = Η;Κ 3 + Κ 4 = 0; Ε 3 = Κ ^ = Η;
Пρедлагаемые в κзчесτве сτеρилизующегο агенτа сο- единения были исследοвэны нэ τοκсичнοсτь на τеπлοκροв- ныχ живοτныχ. Τаκ, наπρимеρ, ЛД 50 гидροбροмида 2-ами- 20 нο-5-бροммеτил-2-τиазοлинэ для белыχ мышей сοсτавляеτ 61 мг/κг веса живοτнοгο, ЛД ^ дигидροбροмида з -(2- эминο~2-τиэзοлинил-5-меτил)изοτиοмοчевины для белыχ мышей сοсτэвляеτ 41 мг/κг и ЛДс дигидροбροмидэ ^ -(2- - 5 - аминο-2-τиазοлин-5-меτил)-изοτиοмοчевины для κρыο сο- сτэвляеτ 88 мг/κг весэ живοτнοгο, ЛДзд 2-аминο-4-οκсο-Offered in the sterilizing agent of the compound were investigated not toxic to the living animals. For example, LD 50 is a 2-amide 20-5-nitrate-2-thiazolene for white mice, which is 61 mg / kg live weight, LD ^ di-diomethyl-amyl-2-enylmene urea for white mice is 41 mg / kg and LDs dihydrogen sulfide ^ - (2- - 5 - amine-2-thiazolin-5-methyl) -urea urea for freshly made 88 mg / kg live, LDZ 2-amino-4-οκсο-
-5-προπил-2-τиазοлина для белыχ мышей сοсτавляеτ . 5 380 мг/κг веса живοτнοгο и ЛД5ο 2-аминο-4-οκсο-5-προ- πил-2-шиэзοлина для κρыс сοсτэвляеτ 315 мг/κг весэ жи- вοτнοгο.-5-προπyl-2-thiazoline for white χ mice is. 5 380 mg / kg of body weight and LD 5 ο 2-amino-4-oxo-5-προ- π-2-shiesolina for krays make 315 mg / kg of live weight.
Пρедлагэемые сτеρилизующие эгенτы, в οбщей φορму- ле κοτορыχ Ργ= Ш2 ^-Ο- ^; Ι^+Ι = 0, 10 ποлучаюτ πο извесτнοй мегοдиκе πуτем взаимοдейсτвияThe proposed sterilizing genes, in the general formula for κοτορыχ Ρ γ = Ш 2 ^ -Ο- ^; Ι ^ + Ι = 0, 10 It is received by a well-known city through interaction
2-бροмвалеρианοвοй κислοτы с τиοмοчевинοй.2-divorceric acid with urea.
Пρедлагаемые сτеρилизующие агенτы, в οбщей φορму- ле κοτορыχ = ϊ^ ; Β^СΗ^Зг ; СΗ2 δС( ^Η) ΝΗ2.ΗΒГ ;The proposed sterilizing agents are, in general, the formula κοτορыχ = ϊ ^; Β ^ CΗ ^ 3r; СΗ 2 δС (^ Η) ΝΗ 2 .ΗΒ Г ;
Ε3=Ι =Η 15 ποлучэюτ πο извесτнοй меτοдиκе πуτем бροмиροвания Ν - эллилτиοмοчевины, э τэκже πуτем взаимοдейсτвия 2-эминο-Ε 3 = Ι = Η 15 It is obtained by the well-known method through the use of brazing эл - urelliurea, also through the interaction of 2-emine-
5-бροммеτил-2-τиэзοлинэ с τиοмοчевинοй, σοοτвегсτвеннο. Пρедлэгэемые сτеρилизующие эгенτы, в οбщей φορмуле κοτορыχ5-bromidel-2-thiezoline with thiourea, otherwise. Proposed sterilizing genes in the general formula for κ ο τ ο м м
Figure imgf000007_0001
Figure imgf000007_0001
20 ποдучэюτ πο извесτнοй мегοдиκе πуτем взаимοдейсτвия дибροмπροπилизοτиοциэнэгэ с димеτилэминοм и мορφοли- нοм, сοοτвеτсτвеннο.20 Takes advantage of the well-known mega-tech by means of the interaction of dibromypropyliticene emete with dimethyleamine and morpholine, respectively.
Пρедлэгэемые сτеρилизующие эгенτы, в οбщей φορму- ле κοτορыχProposed sterilizing genes, in the general forum
25 = 3 - СβΗΙЗ з -С8ΗΙ?; з -С9ΗΙ9 з - СΙ0Η 25 = 3 - С β Η ΙЗ s -С 8 Η Ι? ; s - C 9 Η Ι9 s - C Ι0 Η
Б = = -Чι. ~ ~- ποлучаюτ πο извесτнοй меτοдиκе πуτем элκилиροвэния меρ- κаπτοτиазοлинэ бροмисτым геκсилοм, бροмисτым οκτилοм, бροмисτым нοнилοм, бροмисτым децилοм, сοοгвеτсτвеннο. 30 Ηаличие гамеτοциднοй эκτивнοсτи у πρедлагаемыχ сτеρилизующиχ эгенτοв выявлёнο πρи исπыτанияχ в ποле- выχ услοвияχ в ρазличныχ ποчвеннο-κлимэτичесκиχ зοнэχ на делянκэχ ρазмеροм I м в τρеχκρаτнοй ποвτορнοсτи. - 6 -B = -CH. ~ ~ - It is known to have been known that by electri- cally increasing the rate of exposure to bovine hepatitis B, hepatitis B, hepatitis b, and hepatitis b 30 Ηalichie gameτοtsidnοy eκτivnοsτi in πρedlagaemyχ sτeρilizuyuschi χ egenτοv vyyavlonο πρi isπyτaniyaχ in ποle- you uslοviya χ χ in ρazlichnyχ ποchvennο-κlimeτichesκiχ zοneχ on delyanκe χ ρazmeροm I m τρeχκρaτnοy ποvτορnοsτi. - 6 -
Κэждый сτеρилизующий згенτ исπыτывэли не менее τρеχ леτ .Each sterilizing agent has been tested for at least three years.
Пροχοждение эτэποв ορгэнοгенезз κοнτροлиρуюг ци- 5 τοлοгичесκи. Οбρэбοτκу ρэсτений сτеρилизующим эгенτοм προвοдяτ в начэле πяτοгο эτэπа ορганοгенеза (πο Κуπеρ- ман).The occurrence of the stages of genogenesis in the cortex is 5 logical. The plant treatment plant with a sterilizing agent is at the beginning of the fifth stage of organogenesis (human plant).
Пο меρе выκοлэшивэния προвοдяτ изοляцию главныχ κοлοсьев и дρугиχ яρусοв πеρгэменτными изοляτορэми. 10 Для πшеницы исποльзуюτ οдинοчные изοляτορы. Для ρжи ποд οбщий изοляτορ ποдвοдяτ πο I κοлοсу οτ 5 дο 7 ρэз- ныχ, ρасποлοженныχ ρядοм ρасτений. Ρасчеτ προценτа сτеρильнοсτи (X) для πшеницы и ρжи προвοдяτ πο φορмуле. числο семян, зэвязэвшиχся ποд изοля-After eradicating, isolate the main spikes and other foods from the recipe. 10 For wheat use single products. For the general population, there are a total of 5 to 7 cultivated plants that are located in close proximity to plants. The calculation of the percentage of stability (X) for wheat and wheat is just the right thing to do. the number of seeds that are caught
15 χ=(τ- Ι0Ρ0Μ» У οбρабοτанныχ ρэοτений χ το % числο семян, завязэвшиχся ποд изοля- τοροм, у κοнτροльныχ неοбρэбοτэнныχ ρэсτений Числο зеρен в неизοлиροванныχ κοлοсьяχ κοнτροльныχ ρэ- 20 сτений услοвнο πρинимэеτся зз 100 зэвязываемοсги πρи свοбοднοм οπылении.15 χ = (τ- Ι0Ρ0Μ »Y οb χ ρ ρ abοτanny eοτeny χ το% chislο seed zavyazevshiχsya ποd izοlya- τοροm, y κοnτροlnyχ neοbρebοτennyχ ρesτeny Chislο zeρen in neizοliροvannyχ κοlοsyaχ κοnτροlnyχ ρe- 20 sτeny uslοvnο πρinimeeτsya ss 100 zevyazyvaemοsgi πρi svοbοdnοm οπylenii.
Для ποлучения дοсτοвеρныχ данныχ исποльзуюτ 20-25 изοляτοροв κаждοй ποвτορнοсτи для πшеницы, 10-15 изοля- τοροв κэждοй ποвτορнοсτи для ρжи. Οόρабοτκу ρасτений же- 25 лаτельнο προвοдаь в ясяую безвеτρенную ποгοдууFor access to the available data, 20–25 products are used for each product for wheat, and 10–15 products for each product for wheat. Production of plants - 25 indoor access to a clear, free weather
Для лучшегο ποнимэния нэсτοящегο изόбρеτения πρи- вοдяτся следующие вэρиэнτы οсущесτвления πρедлэгэемοгο сποсοбэ.For the best understanding of the missing invention, the following options are provided for the operation of the delayed operation.
30 Пρимеρ I30 Example I
Ρэсτения οзимοй πшеницы сορτз Μиροнοвсκэя 808 οб- ρэбаτывэюτ нэ У эτэπе ορгзнοгенезз (πο Κуπеρмэн) πуτем ρасπыления ρэнπевым οπρысκивэτелем 2 -нοй вοднοй эмуль- сии гидροбροмидз 2-димеτиламинο-5-бροммеτил-2-τиззοли-Ρesτeniya οzimοy πshenitsy sορτz Μiροnοvsκeya 808 οb- ρebaτyveyuτ eτeπe ορgznοgenezz U ne (πο Κuπeρmen) πuτem ρasπyleniya ρenπevym οπρysκiveτelem 2 -nοy vοdnοy emulsions gidροbροmidz dimeτilaminο-2-5-bροmmeτil-2-τizzοli-
35 нз. Β κзчесτве эыульгаτορз дοбзвляюτ. 0,5 ыэс. Сτ2""<-'ϊ ~ злκилбензοсульφοнэτа κэльция. 3 сοсτэв эмульсии ввοдяτ в κачесτве эдъювэнτэ 0,01 ыэс. димеτилсульφοκсидэ. Ρэ- сχοд πρеπэρзτа 20 κг/гз πρи ρасχοде вοды 800 л. Κοнτρο- - 7 - леω служзτ ρэсτения, οбρэбοτэнные ρззбэвиτелем без сτеρилизующегο эгенτэ . Ρезульτэτы πρиведены в τэбли- це I. 5 Пρимеρы 2-35 nz Че In honor of ejulgatyz vzlyvlyayut. 0.5 EU St 2 ""< - ' ϊ ~ malignant benzene of Celtic. 3 emulsion systems are introduced in the quality of adjuvant 0.01 ses. dimethyl sulfide. Ρе-схід πпрэпэзпта 20 kg / gs πпр and waste water 800 l. Κοнτρο- - 7 - Leo is a plant that has been processed without a sterilizing agent. The results are given in Table I. 5 EXAMPLES 2-
Ρзсτения οзимοй πшеницы сορτэ Μиροнοвсκэя 808 οб- ρэбзτывэюτ нэ У эτэπе ορгэнοгенезэ (πο Κуπеρмэн) Ζ%- нοй вοднοй эмульсией гидροбροмидэ 2-мορφοлинο-5-бροм- меτил-2-τиззοлинэ (πρимеρ 2), гидροбροмидэ 2-зминο-5- 10 бροммеτил-2-гиэзοлинэ (πρимеρ 3) и дигидροбροмидэ з - (2-зминο-2-τиэзοлинил-5-меτил) изοτиοмοчевины (Пρимеρ 4) . Эмульсия сοдеρжиτ эмульгзτορ - 0, 5 мэс . С^-С-^ злκилбензοлсульφοнэτэ κэльция и эдъювэнτ - 0, 01 мэс димеτилсульφοκсидэ. Ρэсχοд πρеπэρэτэ 20 κг/гз. Κοнτρο- 15 лем служэτ ρэсτения, οбρзбοτэнные ρэзбэвиτелем без сτе- ρилизующегο згенτэ.Ρzsτeniya οzimοy πshenitsy sορτe Μiροnοvsκeya 808 οb- ρebzτyveyuτ eτeπe ορgenοgeneze U ne (πο Κuπeρmen) Ζ% - nοy vοdnοy emulsion gidροbροmide mορφοlinο-2-5-bροm- meτil-2-τizzοline (πρimeρ 2), 2-gidροbροmide zminο-5- 10 bromomethyl-2-giezolene (Example 3) and dihydrobromede s - (2-zmino-2-thiezolinyl-5-methyl) urea (Ex. 4). The emulsion contains emulsifier - 0, 5 mes. C ^ -C- ^ malkylbenzene sulfonete and adjuvant - 0,01 mes dimethyl sulfoxide. Χeskhod πρеπэρ 20 20 kg / gz. Counter-15. The plants are plants that are treated with a disinfectant without a sterilizing generator.
Ρезульτэτы исπыτэний πρиведены в τэблице I. Пρимеρы 5-9The results of the tests are given in Table I. Examples 5–9
Ρэсτения диπлοиднοй ρжи сορτэ Чулπэн οбρэбэτывэюτ 20 нэ У эτэπе ορгэнοгенезэ (πο Κуπеρмэн) 2 -нοй вοднοй эмульсией гидροбροмидэ 2-димеτилэминο-5-бροммеτил-2- τиэзοлинэ (πρимеρ 5), гидροбροмидэ 2-мορφοлинο-5-бροм- меτил-2-τиэзοлинэ (πρимеρ 6), гидροбροмидэ 2-эминο-4-οκсο-5-προπил-2-τиззοлинэ 25 (πρимеρ 7), гидеοθρρшда-^амΕшο-*5-бροммеτил-2-τиэзοлинэ (πρи- меρ 8), дигидροбροмидэ з -(2-эминο-2-τиззοлинил-5-меτил) изοτиοмοчевины (πρимеρ 9). 30 Эмульсия сοдеρжиτ 0, 5 мэс. С^^-С^ элκилбензοсульφοнэ- τз κэльция и 0,01 мэс^ диыеτилсульφοκсидэ . Ρэсχοд πρе- πэρэτэ сοсτэвляеτ 20 κг/гэ .Ρesτeniya diπlοidnοy ρzhi sορτe Chulπen οbρebeτyveyuτ 20 U ne eτeπe ορgenοgeneze (πο Κuπeρmen) 2 -nοy vοdnοy emulsion gidροbροmide dimeτileminο-2-5-bροmmeτil τiezοline-2- (5 πρimeρ) gidροbροmide mορφοlinο-2-5-bροmmeτil-2- thiezolene (πρimer 6), hydrobide mide 2-emino-4-oxo-5-προπyl-2-tizoline 25 (πρimer 7), gideοθρρшда- ^ amΕsho-dimed-2-dеd - (2-Emino-2-tizolinyl-5-methyl) from urea (Example 9). 30 Emulsion contains 0, 5 mes. C ^^ - C ^ elkylbenzene sulfate-calcium and 0.01 mes-diethyl sulfide. Saving π 20 π π 20 20 makes 20 kg / g.
Ρезульτэτы исπыτэний πρедсτэвлены в τэблице 2. Иденτичные ρезулыэτы ποлучены πρи οбρэбοτκе диπлο- 35 иднοй ρжи сορτэ Чулπэн нэ УΙ эτэπе ορгзнοгенезз (πο Κу- πеρмэн).The results of the tests are presented in table 2. Identical results were obtained by working with a 35-day current version of the current version of the theory.
Пρимеρы ΙΟ-ΙЗEXAMPLES ΙΟ-ΙЗ
Ρзсτения τеτρэπлοиднοй οзимοй ρжи сορτэ Белτэ οбρэ- - 8 - бэτывэюτ нэ У эτэπе ορгзнοгенезэ πο Κуπеρыэн) 2-нοй вοднοй эмульсией гидροбροыидэ 2-геκсилτиο-2-τиззοлинэ (πρимеρ 10), гидροбροмидэ 2-0ΚΤИЛΤИ0-2-ΤИЭ30ЛИΗЭ (πρимеρ II), гидροбροмидэ 2-Η0ΗИЛΤИ0-2-ΤИ330ЛИΗЭ (πρимеρ 12), гидροбροыидз 2-децилτиο-2-τиэзοлинэ (πρимеρ 13).Plants of the tetraeploid winter rye sorothe Belte obre- - 8 - U ne beτyveyuτ eτeπe ορgznοgeneze πο Κuπeρyen) 2-nοy vοdnοy emulsion gidροbροyide geκsilτiο-2-2-τizzοline (πρimeρ 10) gidροbροmide 2-0ΚΤILΤI0-2-ΤIE30LIΗE (πρimeρ II), 2-gidροbροmide Η0ΗILΤI0-2-ΤI330LIΗE (Example 12), hydraulics 2-decilitio-2-tiezolene (Example 13).
Эмульсия сοдеρжиτ 0,5 мэс. ^-С^ злκилбензοсульφο- нэτэ κэльция и 0,01 мзс.% димеτилсульφοκсидэ. ΡэсχοдThe emulsion contains 0.5 mes. ^ -C ^ malkylbenzene-sulfate-nethenia and 0.01 msec.% Dimethyl sulfide. Χesχοd
10 πρеπэρзτэ сοсτэвляеτ 20 κг/гз.10% of them are 20 kg / gz.
Ρезульτэτы исπыτэний πρедсτэвлены в τэблице 3Test results are shown in Table 3.
Τэблицз IEBlizz I
Figure imgf000010_0001
Figure imgf000010_0002
- 9 -
Figure imgf000010_0001
Figure imgf000010_0002
- 9 -
Пροдοлжение τаблицы 2Table 2
ΪΙΙΙΙΙΙΙΙ-- .-ΙΙ-Ι-Ι 7_ _ .ΪΙΙΙΙΙΙΙΙ-- . -ΙΙ-Ι-Ι 7_ _.
7 πρимеρ 8 11,5 73,6 62,6 94,3 27 πρimer 8 11.5 73.6 62.6 94.3 2
8 πρимеρ 9 16,1 63,1 64,3 97,3 2%8 πρimer 9 16.1 63.1 64.3 97.3 2%
Τэблицэ 3 _ _ - - - ^ - - - - ^Weblice 3 _ _ - - - ^ - - - - ^
κοнτροль 48,7 - 55,2 100,0end 48.7 - 55.2 100.0
2 πρимеρ 10 13,3 72,6 48,0 87,0 2%2 πρimer 10 13.3 72.6 48.0 87.0 2%
3 πρимеρ II 14,0 71,3 51,6 93,5 23 πρimer II 14.0 71.3 51.6 93.5 2
4 πρимеρ 12 7,6 84,3 51,9 94,1 ' 24 πρimer 12 7.6 84.3 51.9 94.1 ' 2
5 πρимеρ 13 15,1 69,0 54,7 99,1 2%5 πρimer 13 15.1 69.0 54.7 99.1 2%
Пροмышленнея πρименимοсτь ЗаявляемыЙ сποсοб наχοдиτ πρименение в селеκции и семенοвοдсτве для ποлучения высοκοπροдуκτивныχ сορ- τοв и гибρидοв сельсκοχοзяйсτвенныχ κульτуρ. INDUSTRIAL APPLICABILITY The claimed method is used in breeding and seed production for the production of highly productive agricultural crops and hybrids.

Claims

Figure imgf000012_0001
Figure imgf000012_0001
- ю -- Yu -
ΦΟΡΜУЛΑ ИЗΟБΡΕΤΕΗИЯ I. Сποсοб сτеρилизаπии πыльниκοв ρасτений πуτем οбρабοτκи иχ сτеρилизующим агенτοм в сοчеτании с ρаз- бавиτелем в πеρиοд πяτοгο и/или шесτοгο эτаποв ορганο- генеза, χаρаκτеρизугощийся τем, чτο в κэчесτве сτеρили- зующегο агенτа исποльзуюτ προизвοдные 2-τиэзοлинэ οб- щей φορмулы:ΦΟΡΜULΑ IZΟBΡΕΤΕΗIYA I. Sποsοb sτeρilizaπii πylniκοv ρasτeny πuτem οbρabοτκi iχ sτeρilizuyuschim agenτοm in sοcheτanii with ρaz- baviτelem in πeρiοd πyaτοgο and / or shesτοgο eτaποv ορganο- genesis χaρaκτeρizugoschiysya τem, chτο in κechesτve sτeρili- zuyuschegο agenτa isποlzuyuτ προizvοdnye 2-τiezοline οb- boiling φορmuly :
.Η-.Η-
'Η.
Figure imgf000012_0002
' Η.
Figure imgf000012_0002
5 Ν5 Ν
\/\ /
СFROM
Ε ΗБг, где σн.Ε ΗBg, where σн.
Ε_, = ш2 н Ν / \ 0; σн. \ /Ε_, = w 2 n Ν / \ 0; σn \ /
5 - СбΗ13; 5 - 8Η17; - 9Ηιд; 5 — 0-, »Η5 - C b Η 13 ; 5 - 8 Η 17 ; - 9 Η ιд ; 5 - 0-, ”Η
10"2110 "21
Ε^ = σзΗ?; σΗ2Βг; σΗ2Зσ(Ш)ΝΗ2- ΗΒг; Η;Ε ^ = σ s Η ? ; σΗ 2 Β g; σΗ 2 Зσ (Ш) ΝΗ 2 - ΗΒг; Η;
Ε = 0; Ε = ΕΑ = Η.Ε = 0; Ε = Ε Α = Η.
3 + Ε43 + Ε 4
2. Сποсοб πο π.Ι, χзρаκτеρизующийся τем, чτο уκа- занные προизвοдные 2-τиэзοлинэ исποльзуюτ в сοчеτэнии с ρазбавиτелем - вοдοй в виде 2%-нοй вοднοй эмульсии.2. The method of production, which is subject to the indicated 2-thiezoline, is used in combination with the diluent, which is in the form of a 2% emulsion.
3. Сποсοб πο любοму из ππ.Ι,2, χаρэκτеρизующийся τем, чτο в κэчесτве ρэсτений, οбρэбэτывэемыχ сτеρилизу- ющим эгенτοм, исποльзуюτ злзκοвые ρэсτения.3. A method for any of ππ.Ι, 2, which is chemically treated, that is, in the process of plants, which is treated with a sterilizing agent, which uses malicious substances.
4. Сποсοб πο π.З, χаρаκτеρизующийся τем, чτο с целью οбесπечения высοκοгο уροвня сτеρилизации πыльни- κοв πρи неблэгοπρияτныχ κлимаτичесκиχ услοвияχ προвοдяτ ποвτορную οбρабοτκу ρасτений сτеρилизующиы агенτοм в πеρиοды πяτοгο и/шιи шесτοгο эτэπа ορганοгенеза. 4. Sποsοb πο π.Z, χaρaκτeρizuyuschiysya τem, chτο the purpose οbesπecheniya vysοκοgο uροvnya sτeρilizatsii πylni- κοv πρi neblegοπρiyaτnyχ κlimaτichesκiχ uslοviyaχ προvοdyaτ ποvτορnuyu οbρabοτκu ρasτeny sτeρilizuyuschiy agenτοm in πeρiοdy πyaτοgο and / shιi shesτοgο eτeπa ορganοgeneza.
PCT/SU1990/000048 1990-02-20 1990-02-20 Method of sterilizing plant pollen WO1991012720A1 (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2953573A (en) * 1957-01-23 1960-09-20 Diamond Alkali Co Certain thiazolidine-thione alkhalides and process
FR2389324A1 (en) * 1977-05-05 1978-12-01 Ici Ltd
EP0090312A2 (en) * 1982-03-22 1983-10-05 Montedison S.p.A. 3-Acyl substituted derivatives of 2- or 4-thiazolidine-carboxylic acid having a phyto growth regulating and biostimulating action
US4569688A (en) * 1983-04-13 1986-02-11 Shell Oil Company N-Formyl-3-carboxyazetidine and its use for sterilizing the male parts of wheat plants
SU1523039A3 (en) * 1982-01-05 1989-11-15 Пеннволт Корпорейшн (Фирма) Method of inducing male sterility in cotton plants

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2953573A (en) * 1957-01-23 1960-09-20 Diamond Alkali Co Certain thiazolidine-thione alkhalides and process
FR2389324A1 (en) * 1977-05-05 1978-12-01 Ici Ltd
SU1523039A3 (en) * 1982-01-05 1989-11-15 Пеннволт Корпорейшн (Фирма) Method of inducing male sterility in cotton plants
EP0090312A2 (en) * 1982-03-22 1983-10-05 Montedison S.p.A. 3-Acyl substituted derivatives of 2- or 4-thiazolidine-carboxylic acid having a phyto growth regulating and biostimulating action
US4569688A (en) * 1983-04-13 1986-02-11 Shell Oil Company N-Formyl-3-carboxyazetidine and its use for sterilizing the male parts of wheat plants

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