WO1991011513A3 - Catechol-o-methyltransferase, polypeptide sequences and dna molecule coding therefor - Google Patents

Catechol-o-methyltransferase, polypeptide sequences and dna molecule coding therefor Download PDF

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Publication number
WO1991011513A3
WO1991011513A3 PCT/FI1991/000025 FI9100025W WO9111513A3 WO 1991011513 A3 WO1991011513 A3 WO 1991011513A3 FI 9100025 W FI9100025 W FI 9100025W WO 9111513 A3 WO9111513 A3 WO 9111513A3
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WO
WIPO (PCT)
Prior art keywords
comt
methyltransferase
catechol
dna molecule
polypeptide sequences
Prior art date
Application number
PCT/FI1991/000025
Other languages
French (fr)
Other versions
WO1991011513A2 (en
Inventor
Ismo Ulmanen
Marjo Salminen
Kenneth Lundstroem
Nisse Kalkkinen
Carola Tilgmann
Anu Jalanko
Hans Soederlund
Original Assignee
Orion Yhtymae Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Orion Yhtymae Oy filed Critical Orion Yhtymae Oy
Publication of WO1991011513A2 publication Critical patent/WO1991011513A2/en
Publication of WO1991011513A3 publication Critical patent/WO1991011513A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C12N9/1011—Catechol O-methyltransferase (2.1.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

A novel isolation procedure was developed that allowed the purification of rat liver and human placenta catechol-O-methyltransferase (EC 2.1.1.6, COMT) enzyme from rat and human sources to a degree sufficient to allow the amino acid sequencing of the enzyme. Recombinant clones directed against the rat liver and human placenta COMT are identified and sequenced. The isolation of pure and recombinant COMT and antibodies thereto, according to the invention, is useful as a medical and pharmacological tool for the development of COMT inhibitors and as a clinical tool for the assay of COMT expression in various disease states.
PCT/FI1991/000025 1990-01-24 1991-01-23 Catechol-o-methyltransferase, polypeptide sequences and dna molecule coding therefor WO1991011513A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US468,720 1983-02-22
US46872090A 1990-01-24 1990-01-24

Publications (2)

Publication Number Publication Date
WO1991011513A2 WO1991011513A2 (en) 1991-08-08
WO1991011513A3 true WO1991011513A3 (en) 1991-10-17

Family

ID=23860969

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FI1991/000025 WO1991011513A2 (en) 1990-01-24 1991-01-23 Catechol-o-methyltransferase, polypeptide sequences and dna molecule coding therefor

Country Status (1)

Country Link
WO (1) WO1991011513A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1252317B1 (en) * 2000-02-04 2007-07-04 Lexicon Genetics Incorporated Human enzymes and polynucleotides encoding the same
US7807392B1 (en) 2003-09-15 2010-10-05 Celera Corporation Lung disease targets and uses thereof
JP2006000023A (en) * 2004-06-16 2006-01-05 Kissei Pharmaceut Co Ltd Human catechol o-methyltransferase crystal
US8927225B2 (en) * 2008-09-26 2015-01-06 The University Of Hong Kong Human catechol-O-methyltransferase (COMT) assay
CN114317482A (en) * 2022-02-21 2022-04-12 郑州伊美诺生物技术有限公司 Method for extracting catechol-O-methyltransferase from fresh pork liver

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
Biochemical and Biophysical Research Communications, vol. 158, no. 3, 15 February 1989, Academic Press, Inc., M.H. Grossman et al.: "Isolation of the mRNA encoding rat liver catechol-O-methyltransferase", pages 776-782 see abstract cited in the application *
Biochimica et Biophysica Acta, vol. 220, 1970, (Amsterdam, NL), R. Gugler et al.: "Reinigung und Charakterisierung einer S-adenosylmethionin: catechol-O-methyltransferase der menschlichen Placenta", pages 10-21 see the abstract cited in the application *
Biomedical Chromatography, vol. 3, no. 3, 3 May 1989, Heyden & Son Ltd, (London, GB) T. Korkolainen et al.: "Purification of rat liver soluble catechol-O-methyltransferase by high performance liquid chromatography", pages 127-130 see the whole article *
Chemical Abstracts, vol. 110, no. 19, 8 May 1989, (Columbus, Ohio, US), R. Backstrom et al.: "Synthesis of some novel potent and selective cathechol. O-methyltransferase inhibitors", see page 736, abstract 172835g, & J. Med. Chem. 1989, 32(4), 841-6 *
Eur. J. Biochem., vol. 21, 1971, (Berlin, DE), P. Ball et al.: "Purification and properties of a catechol-O-methyltransferase of human liver", pages 517-525 see abstract; page 523, left-hand column, last paragraph - right-hand column, paragraph 1; methods *
FEBS, vol. 264, no. 1, May 1990, Elsevier Science Publishers B.V., C. Tilgmann et al.: "Purification and partial characterization of rat liver soluble catechol-O-methyltransferase", pages 95-99 see the whole article cited in the application *
Gene, vol. 72, 1988, Elsevier Science Publishers B.V., A.R. van der Krol et al.: "Antisense genes in plants: an overview", pages 45-50 see introduction *
Gene, vol. 93, September 1990, Elsevier Science Publishers B.V., M. Salminen et al.: "Molecular cloning and characterization of rat liver catechol-O-methyltransferase", pages 241-247 see the whole article *
Nature, vol. 333. 30 June 1988, C. Lichtenstein: "Anti-sense RNA as a tool to study plant gene expression", pages 801-802 see the whole article cited in the application *
Soc. Neurosci. Abstr., vol. 14, no. 2, 1988, M.H. Grossman et al.: "Cloning of rat catechol-O-methyltransferase (COMT)", see abstract 501.4 & 18th Annual Meeting of the Society for Neuroscience, Toronto, Ontario, Canada, November 13-18, 1988, page 1249 see abstract *

Also Published As

Publication number Publication date
WO1991011513A2 (en) 1991-08-08

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