WO1991009527A1 - Insect pathogenic method - Google Patents

Insect pathogenic method Download PDF

Info

Publication number
WO1991009527A1
WO1991009527A1 PCT/FI1989/000240 FI8900240W WO9109527A1 WO 1991009527 A1 WO1991009527 A1 WO 1991009527A1 FI 8900240 W FI8900240 W FI 8900240W WO 9109527 A1 WO9109527 A1 WO 9109527A1
Authority
WO
WIPO (PCT)
Prior art keywords
dsm
fungi
cabbage
larvae
fly
Prior art date
Application number
PCT/FI1989/000240
Other languages
English (en)
French (fr)
Inventor
Heikki Hokkanen
Original Assignee
Kemira Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kemira Oy filed Critical Kemira Oy
Publication of WO1991009527A1 publication Critical patent/WO1991009527A1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungal isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi

Definitions

  • the present invention relates to a method for control of larvae of the large cabbage fly (Delia floralis) and the cabbage-root fly (Delia radicum) in the culture substrate.
  • the pesticides protect the plants only during early summer, from the first gener ⁇ ation of cabbage-root fly larvae.
  • the species of fungi employed in the method according to the invention Beauveria bassiana and Metarhizium anisop ⁇ liae, have been isolated from different types of soil from several different localities. Fungi of the Beauveria bassiana and Metarhizium anisopliae species have earlier been used for insect pathogenic con ⁇ trol. In a prior-known method of biological control" Meta ⁇ rhizium anisopliae fungi are added to the culture substrat to control the common cockchafer.
  • Australian Patent Application 54766/86 also describes a bi logical method and preparation for control of insects.
  • Scarabeid beetle larvae are controlled with the aid of the mixture of Metarhizium anisopliae and Beauveria bassiana by the addition of an effective quantity of the fungi species to the culture substrate.
  • These fungi specie however, have not previously been observed to be effective against cabbage fly larvae, which are difficult to control
  • Beauveria bassiana (Deuteromycetes) belongs to the Fungi I perfecti group, i.e. it is not known to have a sexual repr ductive development stage.
  • the fungus forms white or cream-coloured colonies, occasio ally tinged with pink, which assume a powdery appearance o development of the spores.
  • the spores (conidia) are round and smooth-surfaced, with a diameter of 2-2.5 urn.
  • Beauveria bassiana is a entomopathogenic fungus which occurs commonly in nature and which is capable of infectin representatives of several different groups of instects. I experiments it has been effective against Acridiidae, Hemi ptera, Aphididae, Hymenoptera, Coleoptera, epidoptera, Acarina and Brachycera. In order to act effectively the fungus requires a suitable humidity and temperature, ultra violet radiation has a harmful effect on the spores. For these reasons the soil is the best operating environment for B. bassiana.
  • B. bassiana The minimum temperature which B. bassiana requires in orde to be capable of infecting an insect is 3-8°C.
  • the spore On coming i to contact with the surface of the insect the spore begins to grow a germination tube which, at first mechanically and then with the aid .of enzymatic reactions, penetrates throug the cuticle to the organism of the insect. The insect dies as its vital functions become choked by the growth of the fungal mycelium.
  • B. Bassiana also secretes toxins (beauver- icin) which are lethal to insects. Following the death of the insect the fungus grows in its body saprophytically and upon the mycelium growing out of the insect, forms spores again. Based on the capacity of B. bassiana for saprophytic growth it can be mass cultured on either a solid or liquid artificial nutrient medium.
  • Metarhizium anisopliae belongs, like Beauveria bassiana, to the Deuteromycetes and the Fungi Imperfecti group. The species is readily identified from its spores, which grow in dense, conical clusters and which are dark green in colour and in size 5-8 urn long. The hyphal network is in ⁇ itially white or yellowish, but is ultimately covered en ⁇ tirely under the green spores.
  • M. anisopliae also is an entemopathogenic fungus which occurs commonly in nature and which can be isolated both from insects with green muscardiosis disease and also from soil.
  • the optimum temperatures required by M. anisopliae are slightly higher than for B. bassiana. In order to be able to infect an insect M. anisopliae requires a tempera ⁇ ture of approximately +10°C.
  • M. anisopliae is capable of infecting and killing repre ⁇ sentatives of many different insect groups. It infects and kills insects in the same manner as B. bassiana. M. anis ⁇ opliae produces destruxin toxins and additionally proteases which are also toxic to insects. Different strains and iso ⁇ lates of M. anisopliae, and likewise also of B. bassiana, may differ appreciably in respect of their temperature re ⁇ quirements, pathogenicity and virulence. Beauveria and Metarhizium fungi isolated from the soil are cultured in a fermenter on an ordinary culture medium (PDB medium) at a temperature of 25-35°C and with a pH of 5-7.
  • PDB medium ordinary culture medium
  • the fungi cultured in this way are added to the culture su strate of the plants, for example by treating the root bal of seedlings being transplanted with an aqueous suspension of powder-formulated fungi containing at least 1x10 fungu spores/ml.
  • At least 15 ml/plant of suspension is applied in order to obtain effective control.
  • the unballed roots of seedlings to be transplanted can be immersed in the aqueous suspension.
  • the micro-organisms employed in the invention have been de posited at the Deutsche Sammlung von Mikroorganismen (DSM) Deutschen fur Biotechnologische Anlagen mbH, Grisebac str. 8, G ⁇ ttingen, German Federal Republic, on 1 June 1988
  • the deposit numbers of the Beauveria bassiana strains are DSM 4646, DSM 4647 and DSM 4648, and the deposit numbers o the Metarhizium anisopliae strains are DSM 4649, DSM 4650 and DSM 4651.
  • a field polluted with cabbage fly was planted with cabbage seedlings, the root balls of which had been treated with 15 ml/plant of Beauveria bassiana or Metarhizium anisopli fungus suspensions. The strength of the suspension was 8x10 fungus spores/ml. The other test members were plant treated with 0.1 % Oftanol (effective agent isofenphos) a untreated plants. In autumn after harvesting the crop the numbers of cabbage-root fly larvae and pupae in the cabba roots were counted. The results are given in Table 1. Table 1
  • the pest to be controlled in the experiment was the larvae of the cabbage-root fly (Delia radicum) .
  • Cabbage-root fly eggs were placed in jars containing sterile, damp sand in the centre of which was a whole radish. There were 10 eggs per jar. The eggs were arranged around the radish so that each egg was an approximately equal distance (approx. 3 cm) from the radish.
  • 10 ml of spore suspension was pipetted around and on top of the radish. The concentration of the suspension was 1x10 spores/ml. The concentration was the same also in the jars in which spores of Beauveria (Bb) and Metarhizium (Ma) were used together. In this case half the spores were Beauveria and half were Metarhizium.
  • the fungi were cultured on PDA agar.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Organic Chemistry (AREA)
  • Mycology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Medicinal Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Biomedical Technology (AREA)
  • Botany (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Catching Or Destruction (AREA)
PCT/FI1989/000240 1988-06-28 1989-12-27 Insect pathogenic method WO1991009527A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FI883094A FI80189C (fi) 1988-06-28 1988-06-28 Insektpatogeniskt foerfarande.

Publications (1)

Publication Number Publication Date
WO1991009527A1 true WO1991009527A1 (en) 1991-07-11

Family

ID=8526730

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FI1989/000240 WO1991009527A1 (en) 1988-06-28 1989-12-27 Insect pathogenic method

Country Status (2)

Country Link
FI (1) FI80189C (enrdf_load_stackoverflow)
WO (1) WO1991009527A1 (enrdf_load_stackoverflow)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993009672A1 (en) * 1991-11-22 1993-05-27 Commonwealth Scientific And Industrial Research Organisation Insect pest control
WO1994016566A1 (en) * 1993-01-22 1994-08-04 Shkoda, Vladimir Sergeevich Process for obtaining microbiological preparations for agricultural use, a production line for carrying this out and removable unit for deep culture of the microorganisms
US6660290B1 (en) 2000-10-04 2003-12-09 Myco Pesticides Llc Mycopesticides
CN105901022A (zh) * 2016-04-29 2016-08-31 山东胜伟园林科技有限公司 一种适用于白菜病虫害的白僵菌杀虫剂及其制备方法

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105432669B (zh) * 2014-08-22 2018-11-13 徽县绿源科技开发有限责任公司 一种绿僵菌与生物碱结合防治鳞翅目幼虫的方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU561555B2 (en) * 1982-05-18 1987-05-14 University Of Adelaide, The Fungal composition for control of cockchafers
EP0268177A2 (de) * 1986-11-20 1988-05-25 Bayer Ag Schädlingsbekämpfungs- und Pflanzenbehandlungsmittel
US4925663A (en) * 1987-12-31 1990-05-15 University Of Florida Biological control of imported fire ants with a fungal pathogen

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU561555B2 (en) * 1982-05-18 1987-05-14 University Of Adelaide, The Fungal composition for control of cockchafers
EP0268177A2 (de) * 1986-11-20 1988-05-25 Bayer Ag Schädlingsbekämpfungs- und Pflanzenbehandlungsmittel
US4925663A (en) * 1987-12-31 1990-05-15 University Of Florida Biological control of imported fire ants with a fungal pathogen

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
DERWENT'S ABSTRACT, No. 122 60 C/07; & SU,A,664 995, Publ. week 8007. *
DERWENT'S ABSTRACT, No. 83-793 910/42; & SU,A,982 630, Publ. week 8342. *
DERWENT'S ABSTRACT, No. 874 80 E/41; & SU,A,888 898, Publ. week 8241. *
DIALOG INFORMATION SERVICES, File 125, CLAIMS/US PATENT ABSTRACTS WEEKLY - 03 Apr. 1990 to 12 Jun. 1990, Dialog Accession No. 2045688, STIMAC J.L.: "Biological Control of Imported Fire Ants with a Fungal Pathogen"; & US,A,4 925 663, 15-05-1990. *
DIALOG INFORMATION SERVICES, File 351, World Patent Index 81-90, Dialog Accession No. 4288448, (LUMINUS PTY LTD), "Controlling Soil Inhibiting Pests Esp. Scarabeid Beetle Larvae by Applying Select Strain of Fungus of the Species Metarhizium Anisopliae and/or Beauveria Bassimia to the Soil"; & AU,A,8 654 766, 18-09-1986, 8645, *
J. INSECT PHYSIOL., Vol. 35, No. 2, 1989, I.M. HUXHAM et al.: "Inhibitory Effects of Cyclodepsipeptides, Destruxins, from the Fungus Metarhizium Anisopliae, on Cellular Immunity in Insects", see page 97 - page 105. *
JOURNAL OF INVERTEBRATE PATHOLOGY, Vol. 53, No. 2, 1989, I.M. HUXHAM, "In Vivo and in Vitro Assays for Pathogenicity of Wild-Type and Mutant Strains of Metarhizium Anisopliae for Three Insect Species", see page 143 - page 151. *
STN INTERNATIONAL, File BIOSIS, STN Accession No. 85187353, T.J. POPRAWSKI et al.: "Susceptibility of the Onion Maggot Delia-Antiqua Diptera Anthomyiidae to the Mycotoxin Destruxin E"; & Can Entomol 117 (6), 1985, 801-802. *
STN INTERNATIONAL, File BIOSIS, STN Accession No. 86102182, T.J. POPRAWSKI et al.: "Susceptibility of Delia Antiqua Diptera Anthomyiidae to Eleven Isolates of Entomopathogenic Hyphomycetes"; & Environ Entomol, 14 (5), 1985, 557-561. *
STN INTERNATIONAL, File CA, CHEMICAL ABSTRACTS, Volume 112, No. 11, 3 December 1990, (Columbus, Ohio, US), SANDEEP GUPTA et al.: "Insecticidal Cyclodepsipeptides from Metarhizium Anisopliae", Abstract No. 95143s; & J. CHEM. SOC., PERKIN TRANS., 1, (12), 2347-57, 1989. *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993009672A1 (en) * 1991-11-22 1993-05-27 Commonwealth Scientific And Industrial Research Organisation Insect pest control
WO1994016566A1 (en) * 1993-01-22 1994-08-04 Shkoda, Vladimir Sergeevich Process for obtaining microbiological preparations for agricultural use, a production line for carrying this out and removable unit for deep culture of the microorganisms
US6660290B1 (en) 2000-10-04 2003-12-09 Myco Pesticides Llc Mycopesticides
CN105901022A (zh) * 2016-04-29 2016-08-31 山东胜伟园林科技有限公司 一种适用于白菜病虫害的白僵菌杀虫剂及其制备方法

Also Published As

Publication number Publication date
FI883094A0 (fi) 1988-06-28
FI883094A7 (enrdf_load_stackoverflow) 1989-12-29
FI80189B (fi) 1990-01-31
FI80189C (fi) 1990-05-10

Similar Documents

Publication Publication Date Title
CA2059057C (en) A process and method for production and use of pathogenic fungal preparation for pest control
JP3612071B2 (ja) 植物病原体を制御するためのＳｔｒｅｐｔｏｍｙｃｅｓＷＹＥＣ１０８の使用
Bruck Ecology of Metarhizium anisopliae in soilless potting media and the rhizosphere: implications for pest management
Lewis et al. A formulation of Trichoderma and Gliocladium to reduce damping-off caused by Rhizoctonia solani and saprophytic growth of the pathogen in soilless mix
Zoberi Metarhizium anisopliae, a fungal pathogen of Reticulitermes flavipes (Isoptera: Rhinotermitidae)
Booth et al. Potential of a dried rice/mycelium formulation of entomopathogenic fungi to suppress subterranean pests in small fruits
CZ219096A3 (en) Biologically pure culture of fungal strain nectria pityrodes, biological fungicide, biofungicidal agent, inhibition process of fungal infection of plants, the use of the nectria pityrodes strain and method of detecting a fungicidal micro-organism
US4942030A (en) Biological control of whiteflies and other pests with a fungal pathogen
WO1991009527A1 (en) Insect pathogenic method
KR100379020B1 (ko) 땅콩뿌리혹선충 방제용 천적세균 파스테리아 페네트랜스98-35 세균 및 이의 분리법
EP0923294A1 (en) Insecticidal bacteria
CA1285475C (en) Method of controlling plant feeding mites with the fungus neozygites floridana
Duponnois et al. Effect of different West African species and strains of Arthrobotrys nematophagous fungi on Meloidogyne species
KR102207732B1 (ko) 메타리지움 아니소플리애 ft319 균주 또는 이를 함유하는 배추좀나방 유충 방제용 미생물제제
CA1251725A (en) Method of protecting plants from nematodes
Stanghellini et al. Efficacy of Beauveria bassiana on colonized millet seed as a biopesticide for the control of shore flies
EP0459975B1 (en) Biological control of whiteflies and other pests with a fungal pathogen
KR100379019B1 (ko) 당근뿌리혹선충 방제용 천적세균 파스테리아 페네트랜스99-13 세균 및 이의 분리법
Rahma et al. Potential of endophytic fungi from chilli as bioinsecticides against Tenebrio molitor Lin. and Spodoptera litura Fab. larvae
Siddaraju et al. Exploration of Entomopathogenic Fungi in the Western Ghats
Trizelia Martinius. 2017. Potential of endophytic fungi from chilli as bioinsecticides against Tenebrio molitor Linnaeus, and Spodoptera litura Fabricius, larvae
Joyarani Pegu et al. Metarhizium anisopliae (Metchnikoff) Sorokin as an alternative to chemical pesticides against Odontotermes obesus (Rambur) in the era of organic agriculture.
van den Bosch et al. Microbial control of insects, weeds, and plant pathogens
Van Driesche et al. Methods for biological control of plant pathogens
RANA et al. Biological control of white root rot of poplar with microbial antagonist

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): HU SU US

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): AT BE CH DE ES FR GB IT LU NL SE