WO1991002961A1 - Method of preparing a sample for analysis - Google Patents

Method of preparing a sample for analysis Download PDF

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Publication number
WO1991002961A1
WO1991002961A1 PCT/GB1990/000974 GB9000974W WO9102961A1 WO 1991002961 A1 WO1991002961 A1 WO 1991002961A1 GB 9000974 W GB9000974 W GB 9000974W WO 9102961 A1 WO9102961 A1 WO 9102961A1
Authority
WO
WIPO (PCT)
Prior art keywords
sample
target
substrate material
matrix material
substrate
Prior art date
Application number
PCT/GB1990/000974
Other languages
French (fr)
Inventor
John Stanley Cottrell
Kuldip Kaur Mock
Original Assignee
Finnigan Mat Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Finnigan Mat Ltd. filed Critical Finnigan Mat Ltd.
Priority to US07/835,970 priority Critical patent/US5260571A/en
Priority to EP90909770A priority patent/EP0489021B1/en
Priority to DE69026263T priority patent/DE69026263T2/en
Publication of WO1991002961A1 publication Critical patent/WO1991002961A1/en

Links

Classifications

    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
    • H01J49/0409—Sample holders or containers
    • H01J49/0418—Sample holders or containers for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q

Definitions

  • This invention relates to a method of preparing a sample for analysis, and particularly a sample for analysis by Laser Desorption Mass Spectrometry (LDMS) in which ions are sputtered from a condensed phase sample surface by photon bombardment and are then subjected to mass analysis.
  • LDMS Laser Desorption Mass Spectrometry
  • the matrix material can serve one or more of a plurality of functions. For example it may act as a mediator in transferring energy from the photon bombardment to the sample material molecules; it may provide a physical and chemical environment which, enhances the probability of desorption in the desired state of charge and aggregation; and it may remove excess energy from the desorbed species through evaporation of matrix material molecules from a desorbed cluster of sample material and matrix material ions.
  • the first is to dissolve the sample material together with a 10:1 excess of an inorganic salt in a solvent, place a drop of the solution on the target surface, and evaporate to dryness as described by D.V. Davis et. al. in Analytical Chemistry, 55. 1302 (1983).
  • the sample material deposit is then irradiated with infra-red photons from a pulsed Neodymium YAG laser.
  • the second is to mix equimolar amounts of sample material and an inorganic salt in a droplet of glycerol placed on the target surface as described by L.G. Wright et. al. in Biomedical Mass Spectrometry, 12 159 (1985).
  • the sample mixture is then irradiated with infra-red photons from a continuous wave carbon dioxide laser.
  • Japanese Patent Specification JP62-43562 discloses a sample preparation technique in which a solution of the sample material is mixed with a slurry of glycerol and fine cobalt powder. A droplet of the mixture is then irradiated with ultraviolet photons from a pulsed nitrogen laser.
  • M. Karas et. al. (Int. J. Mass Spectrom. Ion Processes, 28.53 (1987)) describe using a large molar excess of a matrix material which has a strong absorption at the wavelength of the incident radiation.
  • the sample material is dissolved in a solution containing a thousand-fold molar excess of Nicotinic Acid.
  • a drop of the solution is placed on the target surface, evaporated to dryness, and irradiated with 266nm ultraviolet photons from a frequency quadrupled pulsed Neodynium YAG laser.
  • the use of a matrix material which has a strong absorption for the incident photons represents an important distinction between this approach and the first three described because it allows the use of low power densities which increases the probability of desorbing intact molecular ions.
  • a method of preparing a sample for analysis by laser desorption mass spectrometry comprising applying a substrate material to a target for the mass spectrometer to be used; dissolving the sample material in a solvent and applying the solution to the substrate material on the target to be absorbed thereby; dissolving a matrix material in a solvent; and applying the matrix material solution to the substrate material on the target, the solvent used to dissolve the matrix material being such as to release the sample material from the substrate material thereby to provide on the substrate material on the target, after evaporation of the matrix material solvent, an intimately mixed deposit of sample material and matrix material.
  • the matrix material has a strong absorption for the photon bombardment used for mass spectrometry.
  • the substrate material is applied to the target by a technique such as electrospraying which provides a deposit with a large surface area.
  • a substrate material for example Nitrocellulose
  • Nitrocellulose is electrosprayed in known manner onto the central region of a rotated target stage 2 of a mass spectrometer.
  • a mask may be used to ensure that the matrix material 1 is restricted to a well defined area of known diameter.
  • sample material solution 3 for example a 10 molar solution of the peptide in 0.1% aqueous Trifluoroacetic Acid is placed onto the target 2 so as to cover the substrate material deposit.
  • sample material solution 3 is allowed to remain in contact with the substrate material for a period of several seconds so that the peptide molecules will bind to the
  • a microscope slide cover slip can be placed on top of the droplet. so as to encourage it to spread out over the surface.
  • the droplet can be blown off with compressed gas, spun off, or rinsed off by either a stream of solvent or immersion in bulk solvent. Alternatively, the droplet could simply be allowed to dry out, but this would mean that any contaminants would also be left on the target surface.
  • the absorbed sample material solution can then be derivatised using a suitable chemical reagent or enzyme.
  • acetone solvent used for the matrix material will dissolve surface layers of the nitrocellulose substrate material together with the attached peptide molecules, so that sample material and matrix material become intimately mixed in the droplet.
  • the droplet is then allowed to dry naturally by evaporation of the solvent (acetone) or can be assisted to dry by the application of heat or forced air.
  • the loaded target 2 can then be introduced into the source region of a mass spectrometer for analysis of the sample material by bombardment with 266nm photons from a frequency quadrupled Neodymium YAG laser, in known manner.
  • Substrate materials exhibiting highly specific binding properties such as immunoadsorbants can be used.
  • sample material may be transferred directly from an electrophoretic or chromatographic support onto a prepared target.
  • electrophoretic separations and staining are well known to those skilled in the art and descriptions may be found in "Electrophoresis, Theory, Techniques, and Biochemical and Clinical Applications” by A.T. Andrews, Clarendon Press, Oxford, 1986.
  • the techniques for blotting are also well known and are described in "Protein Blotting, Methodology, Research and Diagnostic Applications” edited by B.A. Baldo and E.R. Tovey, Karger, Basel, 1989.
  • a mixture of proteins is denatured using sodium dodecyl sulphate and separated electrophoretically in a slab 21 of polyacrylamide gel.
  • the gel is subsequently stained to reveal the positions of the separated components.
  • the gel 21 is placed in a semi-dry blotting tank 22 on a filter paper 23 and a bottom electrode 24 and one or more targets 25 precoated with a substrate material are placed face down on the upper surface of the gel 21 at the locations of the components or interest.
  • the filter paper 23 and the upper surface of the gel 21 are wetted with a solvent of 25mM Tris, 192 mM glycine, and 20% (v/v) methanol in water.
  • proteins are induced to migrate from the gel 21 towards the targets 25 where they are bound by the substrate material.
  • the targets 25 may then be removed, rinsed, the matrix solution added as described previously, dried, and introduced into the source region of a mass spectrometer for analysis by bombardment with 266 nm photons from a frequency quadrupled Neodymium YAG laser.

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  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Optics & Photonics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Paints Or Removers (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

A sample for analysis by Laser Desorption Mass Spectrometry is prepared by applying a substrate material to a target for the mass spectrometer, dissolving the sample material in a solvent and applying the solution to a substrate material on the target to be absorbed thereby. A matrix material is also dissolved in a solvent and this matrix material in solution is applied to the substrate material on the target. The solvent used to dissolve the matrix material is chosen such as to release the sample material from the substrate so that, after evaporation of the matrix material, there is provided on the substrate material an intimately mixed deposit of sample material and matrix material.

Description

METHOD OF PREPARING A SAMPLE FOR ANALYSIS
This invention relates to a method of preparing a sample for analysis, and particularly a sample for analysis by Laser Desorption Mass Spectrometry (LDMS) in which ions are sputtered from a condensed phase sample surface by photon bombardment and are then subjected to mass analysis.
Many methods of LDMS are known, and a feature common to many is the use of a matrix material in which the analyte (the sample material to be analysed) is dispersed. The matrix material can serve one or more of a plurality of functions. For example it may act as a mediator in transferring energy from the photon bombardment to the sample material molecules; it may provide a physical and chemical environment which, enhances the probability of desorption in the desired state of charge and aggregation; and it may remove excess energy from the desorbed species through evaporation of matrix material molecules from a desorbed cluster of sample material and matrix material ions.
Four techniques for using a matrix material to enhance LDMS have been described as set out below.
The first is to dissolve the sample material together with a 10:1 excess of an inorganic salt in a solvent, place a drop of the solution on the target surface, and evaporate to dryness as described by D.V. Davis et. al. in Analytical Chemistry, 55. 1302 (1983). The sample material deposit is then irradiated with infra-red photons from a pulsed Neodymium YAG laser.
The second is to mix equimolar amounts of sample material and an inorganic salt in a droplet of glycerol placed on the target surface as described by L.G. Wright et. al. in Biomedical Mass Spectrometry, 12 159 (1985). The sample mixture is then irradiated with infra-red photons from a continuous wave carbon dioxide laser.
Thirdly, Japanese Patent Specification JP62-43562 discloses a sample preparation technique in which a solution of the sample material is mixed with a slurry of glycerol and fine cobalt powder. A droplet of the mixture is then irradiated with ultraviolet photons from a pulsed nitrogen laser.
Fourthly, M. Karas et. al. (Int. J. Mass Spectrom. Ion Processes, 28.53 (1987)) describe using a large molar excess of a matrix material which has a strong absorption at the wavelength of the incident radiation. For example, the sample material is dissolved in a solution containing a thousand-fold molar excess of Nicotinic Acid. A drop of the solution is placed on the target surface, evaporated to dryness, and irradiated with 266nm ultraviolet photons from a frequency quadrupled pulsed Neodynium YAG laser. The use of a matrix material which has a strong absorption for the incident photons represents an important distinction between this approach and the first three described because it allows the use of low power densities which increases the probability of desorbing intact molecular ions.
According to this invention there is provided a method of preparing a sample for analysis by laser desorption mass spectrometry, comprising applying a substrate material to a target for the mass spectrometer to be used; dissolving the sample material in a solvent and applying the solution to the substrate material on the target to be absorbed thereby; dissolving a matrix material in a solvent; and applying the matrix material solution to the substrate material on the target, the solvent used to dissolve the matrix material being such as to release the sample material from the substrate material thereby to provide on the substrate material on the target, after evaporation of the matrix material solvent, an intimately mixed deposit of sample material and matrix material.
Preferably the matrix material has a strong absorption for the photon bombardment used for mass spectrometry.
Preferably the substrate material is applied to the target by a technique such as electrospraying which provides a deposit with a large surface area.
The invention will now be described by way of example with reference to the drawing, in which the two figures illustrate two methods of sample preparation in accordance with the invention. Referring to Figure 1, a substrate material 1, for example Nitrocellulose, is electrosprayed in known manner onto the central region of a rotated target stage 2 of a mass spectrometer. A mask may be used to ensure that the matrix material 1 is restricted to a well defined area of known diameter. The electrospray technique is described fully by
C.J. McNeal et. al. in Analytical Chemistry, 5 2036 (1979). A
_5 drop of sample material solution 3, for example a 10 molar solution of the peptide in 0.1% aqueous Trifluoroacetic Acid is placed onto the target 2 so as to cover the substrate material deposit. The sample material solution 3 is allowed to remain in contact with the substrate material for a period of several seconds so that the peptide molecules will bind to the
Nitrocellulose through hydrophobic interaction. If the solution fails to wet the substrate material surface, a microscope slide cover slip can be placed on top of the droplet. so as to encourage it to spread out over the surface. Once the peptide has been immobilised onto the substrate material surface, the droplet can be blown off with compressed gas, spun off, or rinsed off by either a stream of solvent or immersion in bulk solvent. Alternatively, the droplet could simply be allowed to dry out, but this would mean that any contaminants would also be left on the target surface.
The absorbed sample material solution can then be derivatised using a suitable chemical reagent or enzyme.
A droplet of matrix material solution consisting of _2
3 x 10 molar Nicotinic Acid in acetone is then applied to the target to cover the sample material deposit. The acetone solvent used for the matrix material will dissolve surface layers of the nitrocellulose substrate material together with the attached peptide molecules, so that sample material and matrix material become intimately mixed in the droplet. The droplet is then allowed to dry naturally by evaporation of the solvent (acetone) or can be assisted to dry by the application of heat or forced air.
The loaded target 2 can then be introduced into the source region of a mass spectrometer for analysis of the sample material by bombardment with 266nm photons from a frequency quadrupled Neodymium YAG laser, in known manner.
Substrate materials exhibiting highly specific binding properties such as immunoadsorbants can be used.
Referring now to Figure 2, an alternative method of loading sample material onto a target with the substrate material thereon is blotting. By this means, sample material may be transferred directly from an electrophoretic or chromatographic support onto a prepared target. The techniques for performing electrophoretic separations and staining are well known to those skilled in the art and descriptions may be found in "Electrophoresis, Theory, Techniques, and Biochemical and Clinical Applications" by A.T. Andrews, Clarendon Press, Oxford, 1986. The techniques for blotting are also well known and are described in "Protein Blotting, Methodology, Research and Diagnostic Applications" edited by B.A. Baldo and E.R. Tovey, Karger, Basel, 1989.
In one embodiment, a mixture of proteins is denatured using sodium dodecyl sulphate and separated electrophoretically in a slab 21 of polyacrylamide gel. The gel is subsequently stained to reveal the positions of the separated components. The gel 21 is placed in a semi-dry blotting tank 22 on a filter paper 23 and a bottom electrode 24 and one or more targets 25 precoated with a substrate material are placed face down on the upper surface of the gel 21 at the locations of the components or interest. The filter paper 23 and the upper surface of the gel 21 are wetted with a solvent of 25mM Tris, 192 mM glycine, and 20% (v/v) methanol in water. By applying a potential difference of a few tens of volts from a source 26 between the bottom electrode 24 and the conductive targets 25, proteins are induced to migrate from the gel 21 towards the targets 25 where they are bound by the substrate material. The targets 25 may then be removed, rinsed, the matrix solution added as described previously, dried, and introduced into the source region of a mass spectrometer for analysis by bombardment with 266 nm photons from a frequency quadrupled Neodymium YAG laser.

Claims

1. A method of preparing a sample for analysis by laser desorption mass spectrometry, comprising applying a substrate material to a target for the mass spectrometer to be used; dissolving the sample material in a solvent and applying the solution to the substrate material on the target to be absorbed thereby; dissolving a matrix material in a solvent; and applying the matrix material solution to the substrate material on the target, the solvent used to dissolve the matrix material being such as to release the sample material from the substrate material thereby to provide on the substrate material on the target, after evaporation of the matrix material solvent, an intimately mixed deposit of sample material and matrix material.
2. A method as claimed in Claim 1, in which the matrix material has a strong absorption for the photon bombardment used for mass spectrometry.
3. A method as claimed in Claim 1 or Claim 2, in which the substrate material is applied to the target by electrospraying.
4. A method as claimed in any preceding claim, in which the sample material is applied to the substrate material by blotting.
5. A method as claimed in any preceding claim, in which the substrate material is Nitrocellulose.
6. A method as claimed in any one of Claims 1 to 4, in which the substrate material is an immunoadsorbant.
7. A method as claimed in any preceding claim, in which the sample material is derivatised after application to the substrate material and before application of the matrix material solution.
8. A method of preparing a sample for analysis by laser desorption mass spectrometry, substantially as hereinbefore described with reference to Figure 1 or Figure 2 of the drawing.
PCT/GB1990/000974 1989-06-23 1990-06-25 Method of preparing a sample for analysis WO1991002961A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US07/835,970 US5260571A (en) 1989-06-23 1990-06-25 Method of preparing a sample for analysis
EP90909770A EP0489021B1 (en) 1989-08-23 1990-06-25 Method of preparing a sample for analysis
DE69026263T DE69026263T2 (en) 1989-08-23 1990-06-25 METHOD FOR PROCESSING A SAMPLE TO BE ANALYZED

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB8919193.6 1989-08-23
GB8919193A GB2235529B (en) 1989-08-23 1989-08-23 Method of preparing samples for laser spectrometry analysis

Publications (1)

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WO1991002961A1 true WO1991002961A1 (en) 1991-03-07

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Country Status (6)

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US (1) US5260571A (en)
EP (1) EP0489021B1 (en)
JP (1) JPH05503349A (en)
DE (1) DE69026263T2 (en)
GB (1) GB2235529B (en)
WO (1) WO1991002961A1 (en)

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WO1995015001A2 (en) * 1993-11-12 1995-06-01 Waters Corporation Enhanced resolution maldi tof-ms sample surface
GB2334337A (en) * 1998-02-12 1999-08-18 Bruker Saxonia Analytik Gmbh Method for detection of substances by ion mobility spectrometry
US6528320B2 (en) 1993-05-28 2003-03-04 Baylor College Of Medicine Method and apparatus for desorption and ionization of analytes
US6558744B2 (en) 1993-11-12 2003-05-06 Waters Investments Limited Enhanced resolution matrix-laser desorption and ionization TOF-MS sample surface
US6579719B1 (en) 1997-06-20 2003-06-17 Ciphergen Biosystems, Inc. Retentate chromatography and protein chip arrays with applications in biology and medicine
US7074619B2 (en) 1993-05-28 2006-07-11 Baylor College Of Medicine Method and apparatus for desorption and ionization of analytes

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US6020208A (en) * 1994-05-27 2000-02-01 Baylor College Of Medicine Systems for surface-enhanced affinity capture for desorption and detection of analytes
DE4408034C1 (en) * 1994-03-10 1995-07-13 Bruker Franzen Analytik Gmbh Mass spectrometric analysis of proteins sepd. by gel electrophoresis
DE19618032C2 (en) * 1996-05-04 2000-04-13 Bruker Daltonik Gmbh Prepared Maldi sample carriers that can be stored
CA2294449A1 (en) * 1997-06-20 1998-12-30 New York University Electrospraying solutions of substances for mass fabrication of chips and libraries
US6265715B1 (en) 1998-02-02 2001-07-24 Helene Perreault Non-porous membrane for MALDI-TOFMS
US6849847B1 (en) 1998-06-12 2005-02-01 Agilent Technologies, Inc. Ambient pressure matrix-assisted laser desorption ionization (MALDI) apparatus and method of analysis
US6869572B1 (en) * 1999-09-13 2005-03-22 Millipore Corporation High density cast-in-place sample preparation card
US7122790B2 (en) * 2000-05-30 2006-10-17 The Penn State Research Foundation Matrix-free desorption ionization mass spectrometry using tailored morphology layer devices
EP1395824A1 (en) * 2001-05-14 2004-03-10 The Penn State Research Foundation Matrix-free desorption ionization mass spectrometry using tailored morphology layer devices
AU2002305710A1 (en) * 2001-05-25 2002-12-09 Waters Investments Limited Sample concentration maldi plates for maldi mass spectrometry
US7053366B2 (en) 2001-05-25 2006-05-30 Waters Investments Limited Desalting plate for MALDI mass spectrometry
US20040050787A1 (en) * 2002-09-13 2004-03-18 Elena Chernokalskaya Apparatus and method for sample preparation and direct spotting eluants onto a MALDI-TOF target
DK1624933T3 (en) * 2003-05-13 2007-11-05 Ion Beam Applic Sa Method and arrangement for automatic beam assignment in a multi-compartment particle beam treatment plant
DE102004019043B4 (en) * 2004-04-16 2008-08-21 Justus-Liebig-Universität Giessen Preparation method for the micro-area analysis of the composition of substance mixtures
CA2578359A1 (en) * 2004-09-17 2006-11-09 Nanosys, Inc. Nanostructured thin films and their uses
DE102004058555A1 (en) * 2004-12-03 2006-06-08 Qiagen Gmbh Method of concentrating biomolecules near the surface of a crystalline structure
WO2013103417A2 (en) * 2011-11-22 2013-07-11 Purdue Research Foundation Sample deposition chamber for laser-induced acoustic desorption (liad) foils
WO2014135864A1 (en) * 2013-03-05 2014-09-12 Micromass Uk Limited Charging plate for enhancing multiply charged ions by laser desorption

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US7294515B2 (en) 1993-05-28 2007-11-13 Baylor College Of Medicine Method and apparatus for desorption and ionization of analytes
US7413909B2 (en) * 1993-05-28 2008-08-19 Baylor College Of Medicine Method and apparatus for desorption and ionization of analytes
US8748193B2 (en) 1993-05-28 2014-06-10 Baylor College Of Medicine Apparatus for desorption and ionization of analytes
US7074619B2 (en) 1993-05-28 2006-07-11 Baylor College Of Medicine Method and apparatus for desorption and ionization of analytes
US7071003B2 (en) 1993-05-28 2006-07-04 Baylor College Of Medicine Surface-enhanced laser desorption/Ionization for desorption and detection of analytes
US6528320B2 (en) 1993-05-28 2003-03-04 Baylor College Of Medicine Method and apparatus for desorption and ionization of analytes
US7491549B2 (en) 1993-05-28 2009-02-17 Baylor College Of Medicine Apparatus for desorption and ionization of analytes
US7449150B2 (en) 1993-05-28 2008-11-11 Baylor College Of Medicine Probe and apparatus for desorption and ionization of analytes
US6734022B2 (en) 1993-05-28 2004-05-11 Baylor College Of Medicine Method and apparatus for desorption and ionization of analytes
US6558744B2 (en) 1993-11-12 2003-05-06 Waters Investments Limited Enhanced resolution matrix-laser desorption and ionization TOF-MS sample surface
WO1995015001A2 (en) * 1993-11-12 1995-06-01 Waters Corporation Enhanced resolution maldi tof-ms sample surface
WO1995015001A3 (en) * 1993-11-12 1995-09-21 Waters Corp Enhanced resolution maldi tof-ms sample surface
US7105339B2 (en) 1997-06-20 2006-09-12 Ciphergen Biosystems, Inc. Retentate chromatography and protein chip arrays with applications in biology and medicine
US7112453B2 (en) 1997-06-20 2006-09-26 Ciphergen Biosystems, Inc. Retentate chromatography and protein chip arrays with applications in biology and medicine
US6811969B1 (en) 1997-06-20 2004-11-02 Ciphergen Biosystems, Inc. Retentate chromatography—profiling with biospecific interaction adsorbents
US6579719B1 (en) 1997-06-20 2003-06-17 Ciphergen Biosystems, Inc. Retentate chromatography and protein chip arrays with applications in biology and medicine
US7575935B2 (en) 1997-06-20 2009-08-18 Bio-Rad Laboratories, Inc. Retentate chromatography and protein chip arrays with applications in biology and medicine
US6881586B2 (en) 1997-06-20 2005-04-19 Ciphergen Biosystems, Inc. Retentate chromatography and protein chip arrays with applications in biology and medicine
GB2334337A (en) * 1998-02-12 1999-08-18 Bruker Saxonia Analytik Gmbh Method for detection of substances by ion mobility spectrometry
GB2334337B (en) * 1998-02-12 2002-12-24 Bruker Saxonia Analytik Gmbh Method for detection of substances by ion mobility spectrometry
US6144029A (en) * 1998-02-12 2000-11-07 Bruker-Saxonia Analytik Gmbh Method for trace detection by solvent-assisted introduction of substances into an ion mobility spectrometer

Also Published As

Publication number Publication date
GB8919193D0 (en) 1989-10-04
EP0489021A1 (en) 1992-06-10
GB2235529A (en) 1991-03-06
EP0489021B1 (en) 1996-03-27
DE69026263D1 (en) 1996-05-02
JPH05503349A (en) 1993-06-03
US5260571A (en) 1993-11-09
DE69026263T2 (en) 1996-09-19
GB2235529B (en) 1993-07-28

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