WO1989003043A1 - Assay for determining the percentage of free substances in biological fluids - Google Patents

Assay for determining the percentage of free substances in biological fluids Download PDF

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Publication number
WO1989003043A1
WO1989003043A1 PCT/SE1988/000500 SE8800500W WO8903043A1 WO 1989003043 A1 WO1989003043 A1 WO 1989003043A1 SE 8800500 W SE8800500 W SE 8800500W WO 8903043 A1 WO8903043 A1 WO 8903043A1
Authority
WO
WIPO (PCT)
Prior art keywords
ligand
free
oil
percentage
determining
Prior art date
Application number
PCT/SE1988/000500
Other languages
English (en)
French (fr)
Inventor
Erik Ragnar SÖDERGÅRD
Original Assignee
Soedergaard Erik Ragnar
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Soedergaard Erik Ragnar filed Critical Soedergaard Erik Ragnar
Publication of WO1989003043A1 publication Critical patent/WO1989003043A1/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G01N33/5375—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody by changing the physical or chemical properties of the medium or immunochemicals, e.g. temperature, density, pH, partitioning
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors

Definitions

  • the sample is equilibrated with an animal or vegetable oil, e.g. corn oil, together with a labeled version of the ligand.
  • the proportion of the ligand in the oil phase of the biphasic system is determined and the partition coefficient of the free ligand is determined from systems where a buffer is substituted for the sample.
  • the percentage of free ligand in the sample is calculated from the partition coefficient of the free ligand and the proporti ⁇ n ⁇ f the ligand in the ⁇ il phase of the system containing the sample.
  • PBS phosphate-buffered saline
  • % free x T - 2.5 x K x Y P P
  • Y DPM in 100 ul sample from the oil phase and T is to ⁇ tal DPM added to the system.
  • K is the partition coefficient for testosterone which is DPM per volume in the oil phase divided with DPM per volume in PBS phase of blank systems. The partition coefficient for testosterone was found to be 81.5.
  • Spearman's rank correlation coefficient was 0.90 (p ⁇ 0.00001) .

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Endocrinology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
PCT/SE1988/000500 1987-09-28 1988-09-28 Assay for determining the percentage of free substances in biological fluids WO1989003043A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE8703733-9 1987-09-28
SE8703733A SE8703733L (sv) 1987-09-28 1987-09-28 Analysmetod foer bestaemning av procent fri substans i biologiska vaetskor genom jaemviktsfoerdelning i olja/vatten system

Publications (1)

Publication Number Publication Date
WO1989003043A1 true WO1989003043A1 (en) 1989-04-06

Family

ID=20369691

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/SE1988/000500 WO1989003043A1 (en) 1987-09-28 1988-09-28 Assay for determining the percentage of free substances in biological fluids

Country Status (3)

Country Link
AU (1) AU2532188A (sv)
SE (1) SE8703733L (sv)
WO (1) WO1989003043A1 (sv)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2078370A (en) * 1980-06-13 1982-01-06 Nat Res Dev Binding assays
GB2103790A (en) * 1979-03-11 1983-02-23 Technion Res & Dev Foundation Immunoassay method
WO1987001206A1 (en) * 1985-08-21 1987-02-26 William Rudolph Hargreaves Methods and devices for separating, mixing, and detecting components in specific binding assays

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2103790A (en) * 1979-03-11 1983-02-23 Technion Res & Dev Foundation Immunoassay method
GB2078370A (en) * 1980-06-13 1982-01-06 Nat Res Dev Binding assays
WO1987001206A1 (en) * 1985-08-21 1987-02-26 William Rudolph Hargreaves Methods and devices for separating, mixing, and detecting components in specific binding assays

Also Published As

Publication number Publication date
SE8703733L (sv) 1989-03-29
AU2532188A (en) 1989-04-18
SE8703733D0 (sv) 1987-09-28

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