WO1987006851A1 - Dispositif et procede de formation de pores dans des vesicules, ainsi que de chargement et de fusion desdites vesicules - Google Patents

Dispositif et procede de formation de pores dans des vesicules, ainsi que de chargement et de fusion desdites vesicules Download PDF

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Publication number
WO1987006851A1
WO1987006851A1 PCT/US1987/001052 US8701052W WO8706851A1 WO 1987006851 A1 WO1987006851 A1 WO 1987006851A1 US 8701052 W US8701052 W US 8701052W WO 8706851 A1 WO8706851 A1 WO 8706851A1
Authority
WO
WIPO (PCT)
Prior art keywords
chamber
vesicles
electric field
liquid suspension
charge
Prior art date
Application number
PCT/US1987/001052
Other languages
English (en)
Inventor
John Marshall, Iii
Original Assignee
Electropore, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Electropore, Inc. filed Critical Electropore, Inc.
Publication of WO1987006851A1 publication Critical patent/WO1987006851A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
    • B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion

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  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Electromagnetism (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Physics & Mathematics (AREA)
  • Electrochemistry (AREA)
  • Environmental & Geological Engineering (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Water Treatment By Electricity Or Magnetism (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Abstract

Dispositif à haute vitesse et à haute tension utilisant des champs électriques homogènes et uniformes pour traiter des vésicules dans une suspension. Dans une forme d'exécution, des électrodes parallèles (14 et 16) sont utilisées pour effectuer le groupement diélectrophorétique, le préalignement rotatif et l'électrofusion des vésicules ainsi que la création de pores dans lesdites vésicules. Dans une autre forme d'exécution, un dispositif magnétique sans électrode est utilisé pour effectuer le traitement. Les deux formes d'exécution sont alimentées par un système d'alimentation électronique à haute vitesse et haute tension utilisant un système (41) de distribution de rupture à ionisation déclenchée.
PCT/US1987/001052 1986-05-09 1987-05-08 Dispositif et procede de formation de pores dans des vesicules, ainsi que de chargement et de fusion desdites vesicules WO1987006851A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US86153486A 1986-05-09 1986-05-09
US861,534 1986-05-09

Publications (1)

Publication Number Publication Date
WO1987006851A1 true WO1987006851A1 (fr) 1987-11-19

Family

ID=25336075

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1987/001052 WO1987006851A1 (fr) 1986-05-09 1987-05-08 Dispositif et procede de formation de pores dans des vesicules, ainsi que de chargement et de fusion desdites vesicules

Country Status (3)

Country Link
EP (1) EP0266418A4 (fr)
AU (1) AU7433787A (fr)
WO (1) WO1987006851A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4959321A (en) * 1988-03-26 1990-09-25 Preece Alan W Cell fusion apparatus
EP0866123A1 (fr) * 1997-03-21 1998-09-23 Eppendorf-Netheler-Hinz Gmbh Procédé et dispositif de circuit pour l'electropermeation ou l'electroporation de cellules vivantes

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4081340A (en) * 1974-02-02 1978-03-28 Kernforschungsanlage Julich Gesellschaft Mit Beschrankter Haftung Method of and device for increasing the permeability of the skin of cells of living beings
US4289756A (en) * 1976-12-15 1981-09-15 Kernforschungsanlage Julich Gmbh Physiological preparation containing loaded cells of expanded volume in suspension, for preferential accumulation in spleen and liver
US4326934A (en) * 1979-12-31 1982-04-27 Pohl Herbert A Continuous dielectrophoretic cell classification method
US4441972A (en) * 1983-04-08 1984-04-10 D.E.P. Systems, Inc. Apparatus for electrofusion of biological particles
DD213360A1 (de) * 1983-02-02 1984-09-12 Adw Ddr Vorrichtung zur dielektrophoretischen sammlung suspendierter teilchen
US4476004A (en) * 1983-04-08 1984-10-09 D.E.P. Systems, Inc. Apparatus for electrofusion of biological particles
US4578168A (en) * 1984-07-27 1986-03-25 Biotronics Apparatus for fusing live cells with electric fields
US4578167A (en) * 1982-09-28 1986-03-25 Biofusion, Inc. Cell fusion

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4081340A (en) * 1974-02-02 1978-03-28 Kernforschungsanlage Julich Gesellschaft Mit Beschrankter Haftung Method of and device for increasing the permeability of the skin of cells of living beings
US4289756A (en) * 1976-12-15 1981-09-15 Kernforschungsanlage Julich Gmbh Physiological preparation containing loaded cells of expanded volume in suspension, for preferential accumulation in spleen and liver
US4326934A (en) * 1979-12-31 1982-04-27 Pohl Herbert A Continuous dielectrophoretic cell classification method
US4578167A (en) * 1982-09-28 1986-03-25 Biofusion, Inc. Cell fusion
DD213360A1 (de) * 1983-02-02 1984-09-12 Adw Ddr Vorrichtung zur dielektrophoretischen sammlung suspendierter teilchen
US4441972A (en) * 1983-04-08 1984-04-10 D.E.P. Systems, Inc. Apparatus for electrofusion of biological particles
US4476004A (en) * 1983-04-08 1984-10-09 D.E.P. Systems, Inc. Apparatus for electrofusion of biological particles
US4578168A (en) * 1984-07-27 1986-03-25 Biotronics Apparatus for fusing live cells with electric fields

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP0266418A4 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4959321A (en) * 1988-03-26 1990-09-25 Preece Alan W Cell fusion apparatus
EP0866123A1 (fr) * 1997-03-21 1998-09-23 Eppendorf-Netheler-Hinz Gmbh Procédé et dispositif de circuit pour l'electropermeation ou l'electroporation de cellules vivantes

Also Published As

Publication number Publication date
EP0266418A1 (fr) 1988-05-11
AU7433787A (en) 1987-12-01
EP0266418A4 (fr) 1989-02-13

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