WO1986007288A1 - Analytical container - Google Patents

Analytical container

Info

Publication number
WO1986007288A1
WO1986007288A1 PCT/US1986/001241 US8601241W WO1986007288A1 WO 1986007288 A1 WO1986007288 A1 WO 1986007288A1 US 8601241 W US8601241 W US 8601241W WO 1986007288 A1 WO1986007288 A1 WO 1986007288A1
Authority
WO
Grant status
Application
Patent type
Prior art keywords
test
device
member
chambers
base
Prior art date
Application number
PCT/US1986/001241
Other languages
French (fr)
Inventor
Francis K. Mundon
Sean P. O'neill
Daniel H. Zimmerman
Michael J. Fisher
Souza Didicus I. De
Original Assignee
Electro-Nucleonics, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

Links

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates

Abstract

A device (10) for conducting chemical and/or biological reactions includes a base member (12) and a cover member (14) joined together and together defining sealed test chambers. One of the members (12) is of flexible material which is releasably and sealingly joined to the other member (14) to provide access to the test chambers. Lens elements (30) are included on one of the members (14) to image the test chambers. The walls of the test chambers may be made of flexible material so that they may be deformed to permit different portions of the test chambers to be imaged by the lens elements (30). A plurality of conically shaped cavities (20) are formed in the base member (12) so that reaction products accumulate in V-shaped lower portions (20a) of the cavities (20) for better visualization.

Description

ANALYTICAL CONTAINER

BACKGROUND AND BRIEF DESCRIPTION OF THE INVENTION

This invention relates to test devices, and more particularly to a device for conducting a chemical and/or biological reaction. The invention has particular application to biological agglutination reactions.

There is a need for a test device in which a chemical and/or biological reaction may be carried out under the total control of the user, so that reagents or test samples may be introduced into the device and a reaction allowed to proceed to completion, permitting immediate evaluation of the test, preferably by inspection. For example, in immunodiagnostic testing for the presence of an antigen or antibody in a test sample, it would be of great benefit to the physician or technician administering the test if a patient's serum could be easily and simply introduced into a test device for immediate reaction with reagents either already in the device or introduced therein at the time of the test, with ,the test results being immediately visualized for the purpose of diagnosis. The present invention provides such a device, and in apresently preferred embodiment constitutes base and cover members that are joined together and which together define a plurality of sealed test chambers wherein reactions may be conducted. Advantageously, one of the members is of flexible material which is releasably and sealingly joined to the other member to provide access to the test chambers for introduction of a patient's serum into those chambers.

Lens elements may be included on one of the members for imaging the test chambers. Further, the test chambers may be defined by generally conical cavities which enhance the sensitivity by providing wells for the collection of precipitates that are formed. By making the walls of the test chambers of flexible material, those test chambers may be deformed so that different portions thereof are imaged by the lens elements. Further, variations in focal length of the lens elements may be compensated for by selective deformation of the walls of the test chambers, as necessary.

One of the base and cover members advantageously is of thermoplastic rubber material which is inert to substances used in tests. Preferably that material is colored white, which shows up the various colors of reaction products. That member could be clear for light transmission, as desired, or a color could be selected for good reflection of light, typically provided by white coloration. The thermoplastic rubber has an excellent gripping property, which is utilized for sealing purposes. Because of its flexibility, it may be easily removed to provide access to the test chambers. Those test chambers advantageously may be conically shaped, as noted above, so that a precipitated reaction product tends to collect at the bottom of the chamber so that it may be readilyvisualized. To aid in visualization lens elements provided in the device image the bottoms of the conical wells and provide magnification, as desired.

Although it is presently preferred to provide a sealed test chamber which is openable, it is of course possible to utilize a permanently sealed test chamber, incorporating a self-sealing element which may be penetrated for the introduction of serum and reagents, as desired, therein.

Although the invention has been described generally above, it will be more completely understood by reference to the following detailed description of a presently preferred embodiment thereof.

IN THE DRAWINGS

Fig. 1 is a perspective view of a test device embodying the present invention in which a flexible base member is shown partially peeled away from an associated cover member to provide access to test chambers.

Fig. 2 is alongitudinal sectional view through a device of the type in Fig. 1.

Fig. 3 is a plan view of a base member forming a part of the device of Figs. 1 and 2.

Fig. 4 is a sectional view of the base member of Fig. 3, taken along the Section 4-4 in Fig. 3. -4- Fig. 5 is a side view of the base member of Fig. 3.

Fig. 6 is a plan view of a cover member forming a part of the device of Figs. 1 and 2.

Fig. 7 is a longitudinal sectional view of the cover member of Fig. 6, taken along the Section 7-7 in Fig. 6.

DETAILED DESCRIPTION

Referring to Fig. 1, a testing device 10 is shown defined by a base member 12 and a cover member 14. The assembly of Fig. 1 is shown in longitudinal section; in assembled form, in Fig. 2.

Preferably, the base member 12 is of flexible material (it is shown partially "peeled" away from the cover member 10 in Fig. 1) and is of thermoplastic rubber grade, advantageously either pure or oil-modified block copolymer rubbers, such as KRATON thermoplastic rubber grade produced by Shell Chemical Company. Such material is flexible and yet has excellent gripping properties so that it may form an effective seal in the completed device, which may be released as desired for the purpose of adding serum and reagents to test chambers, as will be described below.

Figs. 3 to 5 show the details of the base member 12. It may be of generally white-colored material, so that individual colors of precipitated components may be readily visualized. The base member 12 includes a base section 16 from which a peripheral lip 18 depends from one side thereof' around the periphery of the base member. A plurality of cavities 20 are generally concial in configuration. Although reaction products may tend to accumulate along the sides of the conical cavity, they do tend to concentrate in the V-shaped lower portion 20a of the cavity for better visualization. The cavities 20 terminate at the level of the base section 16 in a ledge 22 bounded by a cylindrical wall 24, which is beveled, as at

24a.

Figs. 6 and 7 show the cover member 14. Preferably, it is formed of transparent rigid plastic material, such as acrylic plastic, formed with a rim 26 extending about the periphery thereof on both sides of an intermediate web section 28. Lens elements 30 depend from one side of the web section 28, while upstanding cylindrical wall members 32 extend from the other side of the base section 28..

The base member 12 and the cover member 14 are interfitted together as shown in Fig. 2. The flexible rubber material of the base member releasably and- sealingly engages the cylindrical walls 32 of the cover member, as shown, so that sealed test chambers 34 are provided.

In use, the device is typically positioned on a surface, such as the top of a table, with the orientation as in Fig. 2, so that rim edge 26a is positioned on the table top. The flexible base member 12 is peeled from the cover member 14, as shown in Fig. 1, so that reagents and serum samples, for example, may be inserted into the now- open test chambers 34. The device is sealed by repositioning the flexible base member 12 to the configuration shown in Fig. 2, and then the device is aggitated and preferably inverted so that it rests on the table top, this time with the edgel 8 positioned on the table top, with the lens elements 30 being upwardly exposed. Reactions proceed within the test chambers 34, and reaction products that precipitate out are collected in the conical cavities 20. Precipitated reaction products may accumulate along the walls of the cavities, but accumulations will occur in the cavity bottom portions 20a, where they may be easily visualized, particularly with the aid of the lens elements 30.

In this regard, the lens elements 30 are normally fabricated so that they image the lower portions 20a of the conical cavities. Any variations in focal length which may occur because of manufacturing tolerances may be compensated for by flexing the cavity walls 20, by hand. Furthermore, by sufficiently manipulating the flexible cavity walls, any desired portion of a cavity wall may be imaged by the corresponding lens element 30.

As noted above, the complete unit may be sealed, if preferred, so that it is pre-packaged with reagents, if desired, or provision may be made for the insertion of reagents and sample serum into the various test chambers by penetration of the flexible base material 12 by syringe. In this case, the base material 12 should be of self- sealing characteristic, so that the syringe penetration does not result in leakage from the test chambers.

As noted above, the opitcal characteristics of the various parts of the device may vary, depending upon the usage desired. Thus the entire device may be transparent, so that it may be positioned within a spectrophotometer, for example, for optical automated testing. In many cases, however, the device will find applicaiton for visualization of the test results by a physician or a technician, who simply views the cavities through the lens elements following completion of a test procedure.

A device for conducting a chemical and/or biological reaction has been disclosed in its presently preferred form. The embodiment described above is susceptible of modification by those skilled in the art. Accordingly, the invention should be taken to be defined by the following claims.

Claims

1. A device for conducting a chemical and/or biological reaction comprising a housing formed at least in part from a base member and a cover member joined together to define a sealed test chamber wherein said reaction may be conducted, at least one of said members being of flexible material releasably and sealingly joined to the other member to provide access to said test chamber.
2. A device for conducting a chemcial and/or biological reaction comprising a housing defining a sealed test chamber wherein said reaction may be conducted, said housing including a lens element formed therein for imaging a portion of said test chamber.
3. A device for conducting a chemical and/or biological reaction comprising a housing defining a sealed test chamber wherein said reaction may be conducted, said test chamber being fomed at least in part of a cavity which is generally conical in shape so that precipitated reaction products tend to accumulate in said cavity.
4. A device according to any one of claims 1 to 3, in which said test chamber is formed at least in part of self-sealing material permitting reaction material to be introduced into said test chamber by syringe.
5. A device according to any one of claims 1 to 3, in which said test chamber is formed at least in part of transparent material for the passage of light therethrough.
6. A device according to any one of claims 1 to 3, in which said housing defines a plurality of test chambers.
7. A device according to any one of claims 1 or 3, in which said housing includes a lens element formed therein for imaging a portion of said test chamber.
8. A device according to claim 7, in which said housing is formed at least in part of flexible material which may be deformed so that different portions of said test chamber are imaged by said lens element.
9. A device according to claim 2, in which said housing is formed with a cavity which is imaged by said lens element.
10. A device according to claim 9 , in which said cavity is generally concial in shape so that precipitated reaction products tend to accumulate in said cavity.
11. A device according to claim 10, in which said housing is formed at least in part of flexible material which may be deformed so that different portions of said cavity are imaged by said lens element.
12. A device according to claim 11, in which said cavity includes a wall structure made of said flexible material.
PCT/US1986/001241 1985-06-07 1986-06-04 Analytical container WO1986007288A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US74234685 true 1985-06-07 1985-06-07
US742,346 1985-06-07

Publications (1)

Publication Number Publication Date
WO1986007288A1 true true WO1986007288A1 (en) 1986-12-18

Family

ID=24984464

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1986/001241 WO1986007288A1 (en) 1985-06-07 1986-06-04 Analytical container

Country Status (2)

Country Link
EP (1) EP0225922A1 (en)
WO (1) WO1986007288A1 (en)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4832422A (en) * 1987-09-08 1989-05-23 Carter-Hoffman Corporation One-piece drawer with integral guide structure
US5214277A (en) * 1992-06-15 1993-05-25 Drennen Iii James K Near-infrared reflectance spectrometer system and related sample cell and sample support
WO1995001559A2 (en) * 1993-07-02 1995-01-12 Evotec Biosystems Gmbh Sample holder and its use
WO2002026377A2 (en) * 2000-09-28 2002-04-04 Beckman Coulter, Inc. Method and apparatus for processing biomolecule arrays
US7169602B2 (en) 2002-12-04 2007-01-30 Applera Corporation Sample substrate for use in biological testing and method for filling a sample substrate
US7332348B2 (en) 2003-02-28 2008-02-19 Applera Corporation Sample substrate having a divided sample chamber and method of loading thereof
US8591836B2 (en) 2003-06-23 2013-11-26 Applied Biosystems, Llc Caps for sample wells and microcards for biological materials

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1196374A (en) * 1968-04-24 1970-06-24 Watson Marlow Instr Ltd Improvements in or relating to the Measurement of the Properties of Fluids
US4251159A (en) * 1979-01-15 1981-02-17 American Hospital Supply Corporation Disposable multi-chamber cuvette
US4391780A (en) * 1981-07-06 1983-07-05 Beckman Instruments, Inc. Container for sample testing
US4431307A (en) * 1981-11-19 1984-02-14 Labsystems Oy Set of cuvettes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1196374A (en) * 1968-04-24 1970-06-24 Watson Marlow Instr Ltd Improvements in or relating to the Measurement of the Properties of Fluids
US4251159A (en) * 1979-01-15 1981-02-17 American Hospital Supply Corporation Disposable multi-chamber cuvette
US4391780A (en) * 1981-07-06 1983-07-05 Beckman Instruments, Inc. Container for sample testing
US4431307A (en) * 1981-11-19 1984-02-14 Labsystems Oy Set of cuvettes

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4832422A (en) * 1987-09-08 1989-05-23 Carter-Hoffman Corporation One-piece drawer with integral guide structure
US5214277A (en) * 1992-06-15 1993-05-25 Drennen Iii James K Near-infrared reflectance spectrometer system and related sample cell and sample support
WO1995001559A2 (en) * 1993-07-02 1995-01-12 Evotec Biosystems Gmbh Sample holder and its use
WO1995001559A3 (en) * 1993-07-02 1995-02-16 Michael Doering Sample holder and its use
WO2002026377A2 (en) * 2000-09-28 2002-04-04 Beckman Coulter, Inc. Method and apparatus for processing biomolecule arrays
WO2002026377A3 (en) * 2000-09-28 2002-09-06 Beckman Coulter Inc Method and apparatus for processing biomolecule arrays
US7070740B1 (en) 2000-09-28 2006-07-04 Beckman Coulter, Inc. Method and apparatus for processing biomolecule arrays
US7169602B2 (en) 2002-12-04 2007-01-30 Applera Corporation Sample substrate for use in biological testing and method for filling a sample substrate
US7332348B2 (en) 2003-02-28 2008-02-19 Applera Corporation Sample substrate having a divided sample chamber and method of loading thereof
US8628730B2 (en) 2003-02-28 2014-01-14 Applied Biosystems, Llc Sample substrate having a divided sample chamber and method of loading thereof
US8591836B2 (en) 2003-06-23 2013-11-26 Applied Biosystems, Llc Caps for sample wells and microcards for biological materials
US9175333B2 (en) 2003-06-23 2015-11-03 Applied Biosystems, Llc Caps for sample wells and microcards for biological materials
US9914123B2 (en) 2003-06-23 2018-03-13 Applied Biosystems, Llc Caps for sample wells and microcards for biological materials

Also Published As

Publication number Publication date Type
EP0225922A1 (en) 1987-06-24 application

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