WO1981000575A1 - Magnetically immobilized microorganism and the use thereof - Google Patents
Magnetically immobilized microorganism and the use thereof Download PDFInfo
- Publication number
- WO1981000575A1 WO1981000575A1 PCT/SE1980/000215 SE8000215W WO8100575A1 WO 1981000575 A1 WO1981000575 A1 WO 1981000575A1 SE 8000215 W SE8000215 W SE 8000215W WO 8100575 A1 WO8100575 A1 WO 8100575A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- fermentation
- reactor
- catalyst
- microbial
- immobilized
- Prior art date
Links
- 244000005700 microbiome Species 0.000 title claims abstract description 36
- 238000000855 fermentation Methods 0.000 claims abstract description 44
- 230000004151 fermentation Effects 0.000 claims abstract description 44
- 239000003054 catalyst Substances 0.000 claims abstract description 30
- 230000000813 microbial effect Effects 0.000 claims abstract description 27
- 239000000758 substrate Substances 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 17
- 239000000126 substance Substances 0.000 claims description 14
- 239000012876 carrier material Substances 0.000 claims description 9
- 229910000859 α-Fe Inorganic materials 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 6
- 239000000648 calcium alginate Substances 0.000 claims description 4
- 235000010410 calcium alginate Nutrition 0.000 claims description 4
- 229960002681 calcium alginate Drugs 0.000 claims description 4
- OKHHGHGGPDJQHR-YMOPUZKJSA-L calcium;(2s,3s,4s,5s,6r)-6-[(2r,3s,4r,5s,6r)-2-carboxy-6-[(2r,3s,4r,5s,6r)-2-carboxylato-4,5,6-trihydroxyoxan-3-yl]oxy-4,5-dihydroxyoxan-3-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylate Chemical compound [Ca+2].O[C@@H]1[C@H](O)[C@H](O)O[C@@H](C([O-])=O)[C@H]1O[C@H]1[C@@H](O)[C@@H](O)[C@H](O[C@H]2[C@H]([C@@H](O)[C@H](O)[C@H](O2)C([O-])=O)O)[C@H](C(O)=O)O1 OKHHGHGGPDJQHR-YMOPUZKJSA-L 0.000 claims description 4
- 239000000696 magnetic material Substances 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 229920002401 polyacrylamide Polymers 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 2
- 239000003960 organic solvent Substances 0.000 claims description 2
- 229920000193 polymethacrylate Polymers 0.000 claims description 2
- 238000005292 vacuum distillation Methods 0.000 claims 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 24
- 239000000047 product Substances 0.000 description 11
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 6
- LQZZUXJYWNFBMV-UHFFFAOYSA-N dodecan-1-ol Chemical compound CCCCCCCCCCCCO LQZZUXJYWNFBMV-UHFFFAOYSA-N 0.000 description 6
- 239000002245 particle Substances 0.000 description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 5
- 239000008103 glucose Substances 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- MWKFXSUHUHTGQN-UHFFFAOYSA-N decan-1-ol Chemical compound CCCCCCCCCCO MWKFXSUHUHTGQN-UHFFFAOYSA-N 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 3
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 3
- 241000588902 Zymomonas mobilis Species 0.000 description 3
- 239000001569 carbon dioxide Substances 0.000 description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
- 229920002678 cellulose Polymers 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- KBPLFHHGFOOTCA-UHFFFAOYSA-N 1-Octanol Chemical compound CCCCCCCCO KBPLFHHGFOOTCA-UHFFFAOYSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N Iron oxide Chemical compound [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- DOIRQSBPFJWKBE-UHFFFAOYSA-N dibutyl phthalate Chemical compound CCCCOC(=O)C1=CC=CC=C1C(=O)OCCCC DOIRQSBPFJWKBE-UHFFFAOYSA-N 0.000 description 2
- 239000000413 hydrolysate Substances 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 238000007885 magnetic separation Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000011368 organic material Substances 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- SCYULBFZEHDVBN-UHFFFAOYSA-N 1,1-Dichloroethane Chemical compound CC(Cl)Cl SCYULBFZEHDVBN-UHFFFAOYSA-N 0.000 description 1
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 1
- NAMYKGVDVNBCFQ-UHFFFAOYSA-N 2-bromopropane Chemical compound CC(C)Br NAMYKGVDVNBCFQ-UHFFFAOYSA-N 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 229960000583 acetic acid Drugs 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 150000001338 aliphatic hydrocarbons Chemical class 0.000 description 1
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 150000004945 aromatic hydrocarbons Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- RDHPKYGYEGBMSE-UHFFFAOYSA-N bromoethane Chemical compound CCBr RDHPKYGYEGBMSE-UHFFFAOYSA-N 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 150000008280 chlorinated hydrocarbons Chemical class 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 229960004756 ethanol Drugs 0.000 description 1
- 239000011554 ferrofluid Substances 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- SZVJSHCCFOBDDC-UHFFFAOYSA-N iron(II,III) oxide Inorganic materials O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 229960000448 lactic acid Drugs 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 239000000661 sodium alginate Substances 0.000 description 1
- 235000010413 sodium alginate Nutrition 0.000 description 1
- 229940005550 sodium alginate Drugs 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- STCOOQWBFONSKY-UHFFFAOYSA-N tributyl phosphate Chemical compound CCCCOP(=O)(OCCCC)OCCCC STCOOQWBFONSKY-UHFFFAOYSA-N 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Definitions
- the present invention relates to a process in the microbial fermentation of an organic medium and to a microbial catalyst suitable for use in said process.
- Immobilized microorganisms have been used earlier for the preparation of i.a. ethanol.
- Cf. The immobilization of microbial cells, subcellular organelles and enzymes in calcium alginate gels" by M. Kierstan and C. Bucke, Bio- technol. Bioeng. 19, 387 (1977).
- Immobilization of a microorganism means that it is associated with a carrier material, e.g. polymeric materials, such as polyacrylamide or calcium alginate.
- the invention relates to a process in microbial fermentation of organic substrate, the fermentation being carried out with a microorganism that is immobilized in a carrier material together with a magnetically influenced substance so that the ratio between the weight of the micro organism and the weight of the magnetically influenced substance is within the range 5-500, preferably 10-200, an the microorganism and the fermented organic substrate being separated upon completion of the fermentation by means of a magnetic field.
- the separation can e.g. be effected by keeping the microbial catalyst (being understood to comprise the micro- organism immobilized in the carrier material together with a magnetically influenced substance) in the reactor by magnetic forces while the reactor is emptied.
- Another way of effecting separation is to catch the microbial catalyst outside the reactor and then return it to the reactor for use in the fermentation of the next substrate batch.
- Still another variant is to keep the microbial catalyst in the reactor by means of a magnetic field while the organic substrate continuously is allowed to flow through the reactor at an adjustable rate.
- One way is to extract continuously the reactor contents during the course of the fermentation with an organic solvent removing the fermentation product formed.
- the solvent must of course be adapted to the fermentation product.
- frequently occuring solvents are alcohols such as ethanol, octanol, decanol and dodecanol; esters such as dibutyl phthalate and tributyl phosphate; aliphatic and/or aromatic hydrocarbons; chlorinated hydrocarbons such as bromoethane, 2-bromopropane and dichloroethane.
- the very combination of immobilized microorganisms and solvent extraction is essential since immobilization protects the microorganisms from being harmfully influenced by the solvent used.
- the microbial catalyst is magnetically influenced the catalyst and the organic substrate can be separated magnetically.
- a magnetic field can keep the microbial catalyst in the reactor while the organic substrate containing the fermentation product is pumped to an extraction unit and thereupon is returned to the reactor.
- the principle of magnetic separation allows a rapid and safe circulation of the organic substrate for a long. time. If instead a filter disc is used as separator, the pores of the filter disc are easily clogged by the suspended particles present leading to operating interruptions
- Another way of keeping the concentration of the fermentation product low in the reactor is to boil off the same continuously from the organic medium in vacuum.
- the method of boiling off the fermentation product in vacuum from the organic substrate has been disclosed earlier for non-immobilized microorganisms (G.R. Cysewski and C.R. Wilke, Bio technol, Bioeng. 19 , 583 (1977)) but said technique has not been considered to be economically justifiable owing to the large amounts of energy required in order to pump away the carbon dioxide formed.
- the microbial catalyst according to the invention is charac- terized in that the microorganism is immobilized in a carrier material together with a magnetically influenced substance, the ratio between the weight of the microorganism and the weight of the magnetic substance being 5-500, pre- ferably 10-200.
- a magnetically influenced substance the ratio between the weight of the microorganism and the weight of the magnetic substance being 5-500, pre- ferably 10-200.
- the carrier material is suitably a polymer, such as calcium alginate, polyacrylamide, polymethacrylate and carragenate whereas the magnetically influenced substance (being understood to be a material attracted by a magnetic field) can be iron filings, magnetite, ferrite, etc., preferably ferrite powder.
- the microorganisms to be used can be of widely different types. Thus, it has been shown that fungi and bacteria well maintain their catalytic activity when applying the present invention but use can also be made of other kinds of microorganisms, such as virus, algae and protozoa.
- a microbial catalyst was prepared in accordance with Example 1, however with the exception that the ferrite powder used in Example 1 and coated with organic material was replaced by 2 grams of ferrite powder.
- a microbial catalyst prepared in accordance with Example 2 in an amount of 2 grams was mixed with 10 ml of 5% glucose solution containing 10 mM calcium chloride. The mixture was stirred in a vessel provided with a cover at 30o and the formation of ethanol was followed by means of gas chromatography.
- the corresponding non-immobilized microorganism as well as microorganism immobilized according to Example 2 but in the absence of ferrite were used.
- the magnetic catalyst is as effective as the non-magnetic one and almost as effective as free microorganism.
- the magnetic microbial catalyst was prepared by means of a strong permanent magnet, was washed with water while still attached to the magnet and then transferred to the next fermentation cycle. This separation could also be carried out in the presence of great amounts of solid particles. As solid particles use were made of sand, glas and wood chips, in the order mentioned, in amounts of 20% by volume.
- a 5% sugar solution in an amount of 10 ml was fermented with a microbial catalyst according to claim 1 (5 grams) in the presence of (test A) and in the absence of (test B) 50 ml of dodecanol.
- a microbial catalyst according to claim 1 5 grams
- the two fermen- tation processes proceeded equally fast but during the later stage of the fermentations the fermentation in test A proceeded about 20% fasterthan the fermentation in test B dependent on the beneficial effect of the removal of the ethanol from the aqueous phase.
- Example 5 A fermentation process similar to that of Example 5 was carried out but using decanol instead of dodecanol while tests A and B were carried out using a starting concentration of 7% by weight of ethanol. During the continued fer- mentation of glucose test A proceeded more than twice as fast as test B. This illustrates the importance of extracting ethanol at high ethanol concentrations.
- a microbial catalyst according to Example 2 was kept magnetically in a reactor of the volume 100 ml through which there was continuously pumped a 10% glucose solution or a 10% glucose solution containing nutrients (0.25% yeast extract, 0.025% NH 4 H 2 PO 4 , 0.0025% MgSO 4 ). The temperature was 30oC.
- a 10% glucose solution or a 10% glucose solution containing nutrients 0.25% yeast extract, 0.025% NH 4 H 2 PO 4 , 0.0025% MgSO 4 .
- the temperature was 30oC.
- the ethanol- yielding capacity as a function of time. In the presence of nutrients the catalyst according to the invention has a good ethanol-yielding capacity during a long time.
- a microbial catalyst according to Example 8 immobilized Zymomonas mobilis but without magnetic substance and free Zymomonas mobilis cells were compared with respect to the ethanol-yielding capacity according to Example 3.
- the capacity of the immobilized bacterium was not influenced nega tively by the incorporation of magnetic material in the carrier material and was about 75% of the capacity of the free bacterium.
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Dispersion Chemistry (AREA)
- Biomedical Technology (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| BR8009026A BR8009026A (pt) | 1979-08-24 | 1980-08-22 | Microrganismo magneticamente imobilizado e sua utilizacao |
| DK182781A DK182781A (da) | 1979-08-24 | 1981-04-23 | Fremgangsmaade ved mikrobiel fermentering af organisk substrat samt mikrobiel katalysator til udoevelse af fremgangsmaaden |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE7907075 | 1979-08-24 | ||
| SE7907075 | 1979-08-24 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO1981000575A1 true WO1981000575A1 (en) | 1981-03-05 |
Family
ID=20338686
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/SE1980/000215 WO1981000575A1 (en) | 1979-08-24 | 1980-08-22 | Magnetically immobilized microorganism and the use thereof |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0041065A1 (enrdf_load_stackoverflow) |
| JP (1) | JPS56501191A (enrdf_load_stackoverflow) |
| BR (1) | BR8009026A (enrdf_load_stackoverflow) |
| DK (1) | DK182781A (enrdf_load_stackoverflow) |
| FI (1) | FI802671A7 (enrdf_load_stackoverflow) |
| WO (1) | WO1981000575A1 (enrdf_load_stackoverflow) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1983002405A1 (en) * | 1982-01-14 | 1983-07-21 | Reed, Thomas, A. | Method and apparatus for separating organic substances from a suspension or a solution |
| WO1983003426A1 (en) * | 1982-03-29 | 1983-10-13 | SCHRÖDER, Ulf | Magnetic carbohydrate particles as carriers for affinity separation purposes, e.g. cell separation |
| US4751397A (en) * | 1982-04-23 | 1988-06-14 | Tokyo Shibaura Denki Kabushiki Kaisha | Power source circuit for base drive circuits for a transistor inverter |
| EP0304143A1 (en) * | 1987-08-19 | 1989-02-22 | Kyoritsu Yuki Co. Ltd. | Water-absorptive resin for microbial carrier |
| EP0313008A3 (en) * | 1987-10-20 | 1989-09-20 | Hoechst Aktiengesellschaft | Magnetic membrane capsules and their use |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010131005A (ja) * | 2008-10-30 | 2010-06-17 | Contig I:Kk | 固定化生体触媒及びその製造方法 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4001197A (en) * | 1975-06-12 | 1977-01-04 | Sala Magnetics, Inc. | Magnetic separation method |
| US4152210A (en) * | 1971-08-27 | 1979-05-01 | Beecham Group Limited | Biologically active materials attached to a ferromagnetic support |
| GB1545545A (en) * | 1975-08-06 | 1979-05-10 | Agronomique Inst Nat Rech | Microbiological reactions |
-
1980
- 1980-08-22 JP JP50193880A patent/JPS56501191A/ja active Pending
- 1980-08-22 EP EP80901624A patent/EP0041065A1/en not_active Ceased
- 1980-08-22 WO PCT/SE1980/000215 patent/WO1981000575A1/en not_active Application Discontinuation
- 1980-08-22 BR BR8009026A patent/BR8009026A/pt unknown
- 1980-08-25 FI FI802671A patent/FI802671A7/fi not_active Application Discontinuation
-
1981
- 1981-04-23 DK DK182781A patent/DK182781A/da unknown
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4152210A (en) * | 1971-08-27 | 1979-05-01 | Beecham Group Limited | Biologically active materials attached to a ferromagnetic support |
| US4001197A (en) * | 1975-06-12 | 1977-01-04 | Sala Magnetics, Inc. | Magnetic separation method |
| GB1545545A (en) * | 1975-08-06 | 1979-05-10 | Agronomique Inst Nat Rech | Microbiological reactions |
Non-Patent Citations (3)
| Title |
|---|
| Biotechnology and Bioengineering, Vol XVIII, issued 1976, Horisberger M, Immobilization of Protein and Polysaccharide on Magnetic Particles: Selective Binding of Microorganisms by Concanavalin A-Magnetite, see p 1647-1651 * |
| Biotechnology Letters 1, issued December 1979, Larsson P O and Mosbach K, Alcohol production by magnetic immobilized yeast, see pages 501-06 * |
| Methods in Enzymology, Vol XLIV, Immobilized Enzymes, issued 1976, edited by Mosbach K, Immobilization on Magnetic Supports, see p 324-325 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1983002405A1 (en) * | 1982-01-14 | 1983-07-21 | Reed, Thomas, A. | Method and apparatus for separating organic substances from a suspension or a solution |
| WO1983003426A1 (en) * | 1982-03-29 | 1983-10-13 | SCHRÖDER, Ulf | Magnetic carbohydrate particles as carriers for affinity separation purposes, e.g. cell separation |
| US4751397A (en) * | 1982-04-23 | 1988-06-14 | Tokyo Shibaura Denki Kabushiki Kaisha | Power source circuit for base drive circuits for a transistor inverter |
| EP0304143A1 (en) * | 1987-08-19 | 1989-02-22 | Kyoritsu Yuki Co. Ltd. | Water-absorptive resin for microbial carrier |
| EP0313008A3 (en) * | 1987-10-20 | 1989-09-20 | Hoechst Aktiengesellschaft | Magnetic membrane capsules and their use |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS56501191A (enrdf_load_stackoverflow) | 1981-08-27 |
| EP0041065A1 (en) | 1981-12-09 |
| DK182781A (da) | 1981-04-23 |
| BR8009026A (pt) | 1981-11-17 |
| FI802671A7 (fi) | 1981-01-01 |
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