WO1980001719A1

WO1980001719A1 - Test paper for simultaneously identifying human and animal pregnancy and sexuality by urine

Info

Publication number: WO1980001719A1
Application number: PCT/JP1979/000036
Authority: WO
Inventors: H Mesaki
Original assignee: H Mesaki
Priority date: 1979-02-16
Filing date: 1979-02-16
Publication date: 1980-08-21

Abstract

The technical field of this invention heretofore belonged to obstetrics in the medicine, and to a proliferation in the veterinary and zootechny, and there exists no technique to simultaneously identify both the presence of pregnancy and the sexuality of a fetal. The conventional method adopted chiefly the serum, blood or urinary hormone of pregnant female and horse, and diagnosed them according to the variation presented at an ovary, i.e., a type of negative reaction by injecting the urine into a domestic rabbit or rat. This, accordingly, required long time in the diagnosis, and was, in addition very complicated technically. The present invention has technically developed remarkably progressively the above described prior art by the application of the catalytic action of benzol derivatives in the hormone similarly excreted into the urine. This invention is based on the mode of life of sexual hormone in which, although the hormone essentially contains only follicle hormone (estrone) in the absence of pregnancy, it will alter strongly to progesterone and specially estradiol and male hormone (androsterone), will gradually change the strongest, as the pregnancy is advances, in the eighth month, and will vanish simultaneously upon delivery as the mode of living sexual hormone, and improves the identifying technique. Fortunately, the sexual hormone is generally colorless and transparent, and presents coloring reaction very sensitively particularly against the benzol derivatives. The present invention is finally based on the fact that the pregnancy and the sexuality are determined by the amount of CH3 group in the chemical formula forming the hormone excreted into the urine. This invention is available for the birth control on the background of population increase and foodstuff resources in human future and of increase of female domestic animal, and also available for the rationalization of stockbreeding through the promotion of productivity and for the study of the protection of wild animals, male domestic animal production increase immediately upon responding of a rule by strengthening the productivity and for the study of the protection of wild animals.

Description

Honorable

Simultaneous pregnancy sex urine test paper

Technical field related to the difference of invention Human doctor. Veterinary. Livestock science

In the past, this type of testing technology was mainly for pregnancy testing only, and no gender-related technology has yet been developed.

Main pregnancy tests in humans

1. Zondeck Mr. Ashheim Urine Injection

The anterior cerebral body weight hormone, which is excreted in urine in the early stages of pregnancy, is injected into immature female mice and the first reaction (mature follicle formation and uterine hyperemia, etc.) and Two reactions (one to several bleeds on the ovary surface) and a third reaction (the appearance of a closed luteal body).

2. Mr. Fried-Man's Urine Injection Ovulation Test

First and second injections into the veins of female rabbits.) Inject a large amount of urine, perform laparotomy 1-2 days later, and develop 1 to several neoluteal bodies and major bleeding in the ovaries. Law.

δ. Abdelhanoleden serodiagnosis method-A method to prove defense enzymes produced in maternal serum. * Serum is added to peptone solution prepared from fresh placenta, and rotation is sometimes measured with a polarization optical rotation meter. The method is to check the change in polarization when pregnant and contraceptive, despite the narrow rotation.

4. Kuho ° 2-Biochemical diagnostic method

A method in which pregnancy urine is treated with benzoate and strong sulfuric acid, and if it shows a fluorescent color at that time, it indicates the presence of follicular hormone and is judged to be pregnant. ―

5. Hoge method

In 1954, he added saturated bromine water to pregnant woman's urine to a certain degree and boiled it.

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i ^p . Two ^ Capelle Nole and Mr. Adore Nole

A large amount of histidine in urine is treated with bromine acetate, and ammonium carbonate and a mixture of ammonia are further added. The mixture is boiled and cooled. Law.

Mr. Bishop Nore and Baumann

In 1954, they stated that 1 drop of 1% hydrogen peroxide solution, 1 drop of 1% hydrogen peroxide solution, 1% of phenylene, hydrazine, hydrochloride and concentrated Add 5 drops of hydrochloric acid, boil for 25 minutes and heat during shaking. When positive, urine does not produce reddish-brown cloud-like precipitates.

The Gutmann method

In 1944, he gained an early high rate in just three hours with a predanandio color reaction excreted in pregnancy urine. The main pregnancy test method in livestock

Pregnant horse serum injection reaction method

1992-2010, Ko and Hart injected the serum of pregnant horses 57-42 days into rats according to the zo and 'a' method. The serum appeared most prominently in this case.

Futechison Blood Holmon Certification Method

In the mare's blood.]? It proved to be a mature egg yolk confection, and it first appeared on pregnancy on the 40th day and existed on its own for 140 days.

Mr. Maken Serum Injection

In 1931, he showed a positive reaction to the injection of pregnant horse serum into rats and rodents, especially between 43 days and about 5 power months of pregnancy. It is said that Roman also promoted the development of male reproductive organs.

Matthen Son Injection Method

In 1954, he injected mares' sera 7 to 1ϋ weeks after breeding into mares.

WIP ,,,

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Talent and Guinolesang law

In 1992, they injected 1 to 10 cubic centimeters of acid-treated urine (120 to 160 liters) into castrated large black rats for 2 days. If estrus occurs at the time, the test is positive, and the hit rate is 50% in the first month of pregnancy, 78.6% in the second month, 91.8% in the third month, and after 4th month. Was 100%.

In 19 δ5, he had previously announced humans, but the amount of honolemon in the horse urine was 550 to 400 liters per liter of horse urine. Fertility was judged to be inferior to the following millimeters, and horses were worthwhile but cattle were unsuitable.

Vaginal contents smear pregnancy test

In 1929, Ryosuke Kurosawa tried vaginal smear histological observations for 150 mares before mating 150 days after mating, and could determine whether or not they were pregnant 7 days after mating. Published a study.

The vaginal oil in the first stage (up to the 10th day) of the father's tail is lightly stained with Giemsa's solution at the time of pregnancy, and its villous epithelial cells contain many neutral polynuclear leukocytes. Contrary to this, during pregnancy, there are many other cells that infect the same fluid, and during the second stage (up to 20 days), many mucous cells appear during pregnancy, and furthermore, during the pregnancy, more mucous cells appear. During period 3 (until 30th :), the uterus is already visually congested and dried or dense mucus adheres and the uterus is blocked.

Atderha lden.E. 1 14--1922.

Die Atider aldeniSclae Reaktion; 1950.

OMPI

V / IFO 3. A der ha 1 den. E. u Bandg e; 1930.

4. Zondek—Die Hormone d e S ovarium a d d. Θ S

H o hyd θ n v or r d er 1 a pp en. 951

5. Lenard—Proe. Soc-θxp, Biol, Med. 1952 · 0. AS chlie im, A "bch, Gjnako 1.144, 1950 ·

Z Ma inine—Ba 11 er. C. W-Nature. 1954.

8. Moor and Barr .—Smear form t h. Θ oral mucoSe in the detection of * .chromoSoiQal 1955. Sex. (Lane θ J g. 9

9. Science—go. Mareh. 195ό ·

10. Time— Boy and irl? 25 j nu. 1950.

11. Chiba Medical Association Journal Vol.15 "I. No.2 3. 19 3 7, Masanobu Kaneko and Kazukuri Yokoyama.

12.Clinical Obstetrics and Gynecology 12 Vol.5 No.4 0, 5, 1955 7 Takashi Ishikawa.

15. Journal of Japanese Society of Obstetrics and Gynecology Vol.2 No.5 860,1957 Jinichi Iwabuchi Vol.44 No.3 89, δ, 1949. Vol.5 No.3,1955.Kawabe Shogo.Tanaka Toru.Vol.2 No.3 No.72,5,1955.Vol.20 No.1 No.5,3,1955.Kikuchi Kazue.

14. Journal of the Kyushu Medical Society 58 8 times 4 3 9, 9, 19 5 7.

15. Alumni Association Bulletin of the University of Tokyo, Obstetrics and Gynecology 20 No. 1, 1 1 9 3 8.

10. Kyudai Medical Journal Vol.20 No.1 No.5, δ, 1955 Kihara Yukio-17. Japanese Veterinary Journal.Medical Journal Vol.14 No.1,1952 Kawase Takesaburo. ~ No. 1 95 1 1 Yukio Sato Takashi Matsumura. 3 70, 1952 2. Tadashi Tsukuba. Ryoichi Yamauchi.

18. Veterinary and Livestock Shimpo 12, 1950. 1 9. Practical veterinary pharmacology 8> 1 9 10 · Gishiro Iijima.

20. New Pharmaceutical Review 1, 195 1. Sumio Umezawa c Technical Sim for Invention Disclosure

It is very important for humans, livestock, and even wildlife to preserve and preserve the species from which they live.

Therefore, scientists around the world have tried a number of judging techniques with the hope of knowing the process, pregnancy and gender, early in conception. The technical process has been in place since the early nineteenth century and has continued into the modern era, but all of them can only be performed by specialist scientists, and all of them are pregnancy tests only. It was all about success in the development of gender identification technical issues.

In other words, the types of pregnancy identification techniques that have been carried out up to today are as follows: (1) Inject the urine and serum of a pregnant animal into a test animal and examine the ovary for abnormalities (swelling and bleeding). [9, (2) stained the uterus and vaginal mucus of the mother's developing fetus to see the abnormalities] ?,

6) is a method based on a quantitative test for luteinized honolemon excreted in urine (for example, “plecnandione-nore”). ■ is the simplest method. The benzoyl derivative was allowed to act on urine in a test tube, and the color reaction was used to make a judgment.

However, not only are these high-grade technologies that can only be performed by specialists, but the technologies are extremely cumbersome, require many days, and have considerable costs. Therefore, the present method was invented under the concept of a simpler method that would allow anyone to judge a housewife or livestock farmer at any time.

That is, the first reagent ό 0% hydrochloric acid (55 ¾ water and then the second Sodium salicylate is adsorbed, and a drop of fresh transparent urine of the human animal is dropped on the adsorbed water. Roasting (coloring reaction that occurs by roasting) enables two birds per stone to detect unexplored pregnancy and gender, which had not been previously developed, in a matter of minutes.

It is advantageous in that it is far superior to the conventional technology.

Effect-As shown in the examples, the hit rate is high as shown below for both pregnancy and gender. Ie

Premature pregnancy rate: 98.15% for humans 90.87% for livestock. Sex Prevalence 90.58% for humans 95.37% for livestock. Especially in the area of appraisal, in the case of pregnancy, not only is the effect of appraisal when the status of pregnancy is unknown at all, but also the gender can be appraised at the same time as pregnancy. The idea is O

It is of great value to clarify the fact that little has been done about gender appraisal in the past 1). (Ii) brief description of the drawings;

As shown in the first drawing, a white paper with a length of 20 cm and a width of 20 cm is used, and as thick as possible blotter paper, three diameter ^ 7 cm It has a circular frame with an entrainment hole, in which the first and second reagents described above are adsorbed, and then a drop of urine from the subject is dropped in the center of the circle and heated. The color reaction of males and females (¾..) Appears around the urine droplet, and the color is used to judge the color reaction.

The dotted line in the circle in this figure indicates the part where the reagents necessary for the appearance of the color reaction have been adsorbed in advance.

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Vv IPO The second drawing illustrates the appraisal method by way of illustration.

In other words, when male (male) sex (δ) is reached, the outer ring of urine dripped has a circular saw-tooth shape as shown on the left end of the figure. A dark broad purple or brown color reaction appears, In the case of (female) sex (s), the outer ring of the urine similarly exhibits a pale narrow narrow purple or pale yellow color reaction as shown in the middle figure.

On the other hand, in the case of pregnancy or non-pregnancy, no ring color appears at all, as shown in the right-most figure, and the entire area within the circle appears only as a slightly yellow or rare red (pink). An appraisal can be made immediately. E example

(1) Livestock (cow :) Early pregnancy test

This example is 1951.] In the 22nd year of 9 197 2 years, there were 102 cases of input and 131 cases of livestock. . However, the case of singleton conception is assumed.

In addition, during the early appraisal period, both subjects were tested with a target of 5 months of pregnancy, and the hit rate was 98.15% for humans and livestock for

90.87%.

(2) Examples of livestock (cow) gender identification

This example is 1951.] In the 22nd year of 91972, 409 human cases and 31 livestock cases were carried out for a total of 750 cases. However, the case of singleton conception is assumed.

The hit rate was 96.58% for humans and 95.37% for livestock.

OMPI

WIPO, Livestock Early Pregnancy Prevalence Report Table People Livestock (Cow)

Numbers in parentheses indicate inaccurate numbers

ί ',''· PI Monthly Gender Score Report

Livestock (Cow :) Monthly Gender Prediction Report

Numbers in parentheses indicate non-hit numbers.

WiPO To Industrial Use

We recognize that the following three methods and benefits can be gained.

(1) The prospect of rationalizing dairy and cattle management can be confirmed.

To confirm early fertility and gender after 1-2 months of artificial insemination in the breeding season of spring and autumn in dairy cows, it is necessary to make a prospect of streamlining management for the year. _Q to help you get

In addition, dairy cows have a low fertilization ratio of about 10% to 20% in all countries, but these cows are also discarded if they are not pregnant at the early stage after artificial insemination. Processing has the benefit of saving enormous feed costs and labor during the breeding season o

(2) Contribute to the conservation of zoo and wildlife reserve species.

In other words, in zoos and protected areas around the world, it is very important to check for early fertility in order to save the threat of the threat of precious animal species to the extinction. No. In this regard, this method is very useful because it is simple and has a high accuracy. For example, it was studied at the Ueno Zoo in Japan and in the creation of a radiopon at the Kobe Zoo, but it was all over.

6) It also helps coordinating nuclear families to solve population problems.

Production method

There are also two methods: selection of appraisal paper and preparation and production of reagents. The blotting paper used is a commercially available blotting paper that has a particularly strong water-absorbing power. A circular frame is provided at each of the three places as shown in the figure to determine the location of urine drops.

Make a suitable glass infusion device for a given drug.]? Restrict the dose

It must be noted that sodium salicylate, which is the second reagent, is long in nature due to the nature of the benzoyl group.

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/ \ Vv: IPPOO ~ When kept in an aqueous solution, the color often changes to a pale brown color and confuses the color reaction. Therefore, always try to use fresh and transparent, and scaly. Crystalline sodium salicylate should always be stored dry.

how to use

1) When using a test paper, drop a drop of urine from the subject's animal in a Komagome pit or a glass container of the same diameter in the center of the circle shown in the figure. When the size of the drop reaches almost the size of a Japanese coin lending coin (2.2 centimeters) due to the surface tension, both ends of the appraisal paper are removed. After holding and heating on a coal fire, electric heater or oil stove, the contact ring of the first and second reagents adsorbed first after about 2 to 5 minutes. Since a color reaction appears, the color is used to determine whether or not a child is pregnant and the sex of a male or female.

In this case, the heating is always limited to the extent that the white part inside and outside the circle of the appraisal paper is burnt to a fox color. However, care should be taken when heating too much, as it will turn black and misjudgment the identification.

These two reagents are harmless to humans and animals.

Claims

Scope of the request Conventional human and livestock pregnancy tests simply looked at humans. Livestock was limited to livestock, and there was no gender test at all. In general, it is about one to two months of pregnancy, and it is already possible to determine the gender status and gender at the same time. In addition, conventional testing techniques require complicated procedures such as injection of pregnancy urine and the staining of genital mucus, which require quantification of luteinized honolemon. The method is to apply a total of three drops, one drop each of the specified first and second reagents and the urine to be tested, on a specific white blotting paper, and to use the color of the chemical color reaction.]? Was set as the claims.

(1) Reagent properties This reagent consists of two types. The first reagent is

Use 60% aqueous hydrochloric acid (35%) in water, and use 1% sodium benzoate as sodium reagent for the second reagent.

In particular, the second reagent has never been used before. The natural rare purple color combines with sex homone, depending on gender.]) Contrary to the appearance of favorable shades, simple salicy-noreic acid is insoluble (1; 500 and There is a disadvantage that it does not melt at high temperatures.

(2) The chemical formula is as follows: The male (tt) honolemon with two CH ₃ hydrocarbons appears in the most intense purple or brown color, followed by a female (female) :) Sex honolemon has a rare purple or yellow color, and an estrus with one at the time of non-fertility has only about 1 potency of estradionore. It does not show any perceptive colors.

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CbH4000Na + 2HOl = N cl) t + O "H4O0OH + H £ O

(OHs) f. OH. C ₆ H4000 (0E _S ) ί + 0Η2 + 0 (An tt Sun) β GH3OH2 O _e H40000H3 + OH3

Η ΟΗι .OH.O 0eH40000H ₈ + 0Hx + 0