UY32210A - IMPROVED ACTIVATION PROCESS FOR THE PREPARATION OF SOLUBLE-PROTEIN POLYACARIUM CONJUGATES AND THEIR PURIFICATION BY HYDROPHOBIC INTERACTION - Google Patents
IMPROVED ACTIVATION PROCESS FOR THE PREPARATION OF SOLUBLE-PROTEIN POLYACARIUM CONJUGATES AND THEIR PURIFICATION BY HYDROPHOBIC INTERACTIONInfo
- Publication number
- UY32210A UY32210A UY0001032210A UY32210A UY32210A UY 32210 A UY32210 A UY 32210A UY 0001032210 A UY0001032210 A UY 0001032210A UY 32210 A UY32210 A UY 32210A UY 32210 A UY32210 A UY 32210A
- Authority
- UY
- Uruguay
- Prior art keywords
- polysaccharide
- protein
- hydrophobic interaction
- purification
- polyacarium
- Prior art date
Links
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
Abstract
Los presentes inventores, describen una mejora en la técnica de activación del antígeno polisacarídico activado con el reactivo tetragluoroborato de 1-ciano-4-(dimetilamino) piridinio (CDAPBF4) previamente reportada (Lees et al., 1996) y un proceso de purificación del conjugado polisacárido-proteína obtenido por éste método directamente a partir de la mezcla de reacción obtenida por el mencionado proceso, en un solo paso mediante cromatografía de interacción hidrofóbica sobre geles de matrices hidroxipropiladas o hidroxialquiladas, que permite la obtención del conjugado polisacárido-proteína libre tanto de polisacárido como de proteína sin conjugar, simplificando el proceso general de obtención del producto y brindando un producto con mejores características para ser utilizado como inmunógenoThe present inventors describe an improvement in the technique of activation of the activated polysaccharide antigen with the 1-cyano-4- (dimethylamino) pyridinium tetragluoroborate reagent (CDAPBF4) previously reported (Lees et al., 1996) and a purification process of the polysaccharide-protein conjugate obtained by this method directly from the reaction mixture obtained by said process, in a single step by hydrophobic interaction chromatography on hydroxypropylated or hydroxyalkylated matrix gels, which allows obtaining the polysaccharide-free protein conjugate both of polysaccharide as an unconjugated protein, simplifying the general process of obtaining the product and providing a product with better characteristics to be used as an immunogen
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| UY0001032210A UY32210A (en) | 2009-10-29 | 2009-10-29 | IMPROVED ACTIVATION PROCESS FOR THE PREPARATION OF SOLUBLE-PROTEIN POLYACARIUM CONJUGATES AND THEIR PURIFICATION BY HYDROPHOBIC INTERACTION |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| UY0001032210A UY32210A (en) | 2009-10-29 | 2009-10-29 | IMPROVED ACTIVATION PROCESS FOR THE PREPARATION OF SOLUBLE-PROTEIN POLYACARIUM CONJUGATES AND THEIR PURIFICATION BY HYDROPHOBIC INTERACTION |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| UY32210A true UY32210A (en) | 2010-01-29 |
Family
ID=43447871
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| UY0001032210A UY32210A (en) | 2009-10-29 | 2009-10-29 | IMPROVED ACTIVATION PROCESS FOR THE PREPARATION OF SOLUBLE-PROTEIN POLYACARIUM CONJUGATES AND THEIR PURIFICATION BY HYDROPHOBIC INTERACTION |
Country Status (1)
| Country | Link |
|---|---|
| UY (1) | UY32210A (en) |
-
2009
- 2009-10-29 UY UY0001032210A patent/UY32210A/en unknown
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