USRE34414E - Physiologically active substance P-23924, its production and use - Google Patents
Physiologically active substance P-23924, its production and use Download PDFInfo
- Publication number
- USRE34414E USRE34414E US07/693,107 US69310791A USRE34414E US RE34414 E USRE34414 E US RE34414E US 69310791 A US69310791 A US 69310791A US RE34414 E USRE34414 E US RE34414E
- Authority
- US
- United States
- Prior art keywords
- methoxy
- compound
- sub
- hydrogen
- methanol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C323/00—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups
- C07C323/50—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and carboxyl groups bound to the same carbon skeleton
- C07C323/51—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and carboxyl groups bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton
- C07C323/57—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and carboxyl groups bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton the carbon skeleton being further substituted by nitrogen atoms, not being part of nitro or nitroso groups
- C07C323/58—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and carboxyl groups bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton the carbon skeleton being further substituted by nitrogen atoms, not being part of nitro or nitroso groups with amino groups bound to the carbon skeleton
- C07C323/59—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and carboxyl groups bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton the carbon skeleton being further substituted by nitrogen atoms, not being part of nitro or nitroso groups with amino groups bound to the carbon skeleton with acylated amino groups bound to the carbon skeleton
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C50/00—Quinones
- C07C50/26—Quinones containing groups having oxygen atoms singly bound to carbon atoms
- C07C50/32—Quinones containing groups having oxygen atoms singly bound to carbon atoms the quinoid structure being part of a condensed ring system having two rings
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
- C12P7/26—Ketones
Definitions
- the present invention relates to Physiologically Active Substance P-23924, its production and use.
- Protocollagen prolyl hydroxylase is an enzyme which specifically hydroxylates the proline moiety of the protocollagen synthesized by ribosome in animal cells and is an important and rate-limiting factor in the biosynthesis of collagen.
- iron chelators e.g. ⁇ , ⁇ '-dipyridyl etc.
- SH enzyme inhibitors e.g. p-chloromercury benzoate etc.
- certain heavy metals e.g. Cu ++ , Zn ++ , etc.
- a substance could be used for the prevention and treatment of organ fibrosis accompanied by an excessive accumulation of collagen such as arteriosclerosis, liver cirrhosis, scleroderma, keloid, rheumatic arthritis, pulmonary fibrosis, etc.
- the present invention relates to (1) a compound represented by the formula: ##STR3## wherein R 1 is hydrogen, methyl or hydroxymethyl, R 2 is hydrogen or methoxy, R 3 is hydroxy or methoxy and R 4 is hydrogen or a group ##STR4##
- a method of producing Physiologically Active Substance P-23924 A, B, C, D, E and/or F which comprises cultivating a microorganism belonging to the genus Streptomyces and capable of elaborating Physiologically Active Substance P-23924 A, B, C, D, E and/or F in a medium to cause said microorganism to elaborate and accumulate Physiologically Active Substance P-234924 A, B, C, D, E and/or F and recovering the same substance from the resulting culture broth.
- the microorganism employed in accordance with the present invention may be any strain of microorganism that belongs to the genus Streptomyces and is able to elaborate Physilogically Active Substance P-23924 A, B, C, D, E and/or F (which will sometimes be referred to briefly as a P-23924 producing strain.
- Streptomyces sp. No. 23924 (hereinafter referred to briefly as Strain No. 23924) which was isolated from a soil sample collected in Ishigakijima, Okinawa Prefecture, Japan.
- Monopodially extending from vegetative mycelia are short sporulated filaments which are spiral or sometimes incompletely spiral, open-loop or hook-shaped, without whorl formation.
- Mature spore chains generally show 5 to 10 spores in chains.
- the spores are either cylindrical or elipsoidal and range in size from 0.4 to 0.7 by 0.7 to 1.2 microns, with smooth surfaces.
- G degree of growth
- AM aerial mycelium
- SP production and color of soluble pigments
- Microorganisms of the genus Streptomyces are generally liable to change in characteristics and can be easily caused to undergo mutation by artificial procedures such as X-ray, ultraviolet ray or radioactive ray irradiation or by a treatment employing a mutagenic agent. Such mutants, only if they are able to produce P-23924 A, B, C, D, E and/or F, can all be employed for the purposes of the present invention.
- the medium for cultivation of P-23924 producing strains may be a liquid medium or a solid medium and it is generally advantageous to conduct shake culture or aerobic submerged culture using a liquid medium.
- the medium may be of any kind only if it is suitable for growth of Actinomyces and for the production thereby of P-23924A, B, C, D, E and/or F.
- sources of carbon there may be employed glucose, lactose, glycerin, starch, sucrose, dextrin, molasses, organic acids (e.g. acetic acid, tartaric acid, etc.) and so on.
- sources of nitrogen there may be employed peptone, Casamino acid (Difco, U.S.A.), N-Z Amine A (Sheffield, U.S.A.) and other protein hydrolysates, yeast extract, malt extract, soybean meal, corn steep liquor, amino acids (e.g. aspartic acid, glutamic acid, etc.), various ammonium salts (e.g. ammonium sulfate, ammonium chloride, etc.) and so on. It is also possible to add inorganic salts such as various phosphates (e.g. sodium dihydrogen phosphate, potassium monohydrogen phosphate, etc.), metal salts (e.g.
- the output of P-23924A, B, C, D, E and/or F may at times be increased by adding a defoaming agent such as silicone, polypropylene glycol ether derivative [e.g. Actocol (Takeda Chemical Industries, Ltd., Japan), etc.], soybean oil, etc. to the culture medium.
- a defoaming agent such as silicone, polypropylene glycol ether derivative [e.g. Actocol (Takeda Chemical Industries, Ltd., Japan), etc.], soybean oil, etc.
- the cultivation temperature and time, the pH of the medium, and other cultural conditions depend on the particular strain and the composition of the medium, they should be so selected and controlled that the output of P-23924 A, B, C, D, E and/or F will be maximal. It is in many cases desirable to conduct aerobic culture at about 20° to 40° C. for 24 to 240 hours while the pH of the medium is maintained at about 4 to 9. Recovery of Physiologically Active Substance P-23924 A, B, C, D, F and/or F from the culture broth can be easily accomplished by utilizing various procedures in a combination suited to the properties of the same substance.
- P-23924A, B, D and F are easily soluble in dimethyl sulfoxide, dimethylformamide, pyridine and 5% aqueous sodium hydrogen carbonate; soluble in methanol, dioxane and acetic acid; slightly soluble or insoluble in water, acetone, chloroform, n-hexane and petroleum ether.
- P-23924C is easily soluble in dimethyl sulfoxide, dimethylformamide, pyridine and 5% aqueous sodium hydrogen carbonate, soluble in methanol, dioxane, acetone and acetic acid; and slightly soluble or insoluble in water, chloroform, n-hexane and petroleum ether.
- P-23924E is easily soluble in dimethyl sulfoxide, dimethylformamide, pyridine, 5% aqueous sodium hydrogen carbonate and methanol, soluble in dioxane and acetic acid, and slightly soluble or insoluble in water, chloroform; n-hexane and petroleum ether.
- FIGS. 1 through 6 The wavelengths (nm) and E 1 cm 1% values giving absorption peaks immediately after dissolution in the respective solvents are presented in Table 1.
- FIGS. 1 through 6 ---represents the values measured in methanol,------the values in 0.1N HCl-90% aqueous methanol,------the values measured in 0.1N NaOH-90% aqueous methanol.
- P-23924 A, B, C, D, E and F are acidic and fat-soluble substances occurring as crystals, crystaline powders or powders which are yellow to yellow-orange or orange-yellow in color.
- P-23924 A, B, C, D, E and F are considered to be a novel substances.
- compound .[.(II).]. .Iadd.(III) is capable of forming salts.
- the conventional method can be used for producing such salts.
- the salts include, for example, salts with calcium, magnesium, barium sodium, potassium, manganese, iron, copper, zinc, etc.
- the reduction is conducted by the manner per se known, such as catalytic reduction, electrolytic reduction and reduction using a reducing agent.
- the reduction is practiced by catalytic reduction, i.e. by reduction in the presence of a catalyst
- a routine solvent e.g. alcohol (e.g. methanol, ethanol, isopropanol, etc.), dioxane, tetrahydrofuran, dimethylformamide or a mixture of such solvents[ and hydrogen gas is bubbled into the solution in the presence of a catalyst [e.g. Raney nickel, palladium on carbon, palladium barium carbonate, platinum oxide, rhodium complex, Raney type catalyst of cobalt, Raney type catalyst of iron, Raney type catalyst of copper].
- a catalyst e.g. Raney nickel, palladium on carbon, palladium barium carbonate, platinum oxide, rhodium complex, Raney type catalyst of cobalt, Raney type catalyst of iron, Raney type catalyst of copper.
- This reaction is carried out at a temperature of 20° to 100° C., desirably at 60° to 80° C. for 10 minutes to 10 hours. While the reaction proceeds readily at atmospheric pressure, one may conduct it at elevated pressures between 5 to 200 kg/cm 2 .
- the compound (III) is treated with a reducing agent [for example, a metal hydride (e.g. lithium aluminum hydride, sodium borohydride, tributyltin hydride, etc.), alkali metal (e.g. lithium, sodium, etc.), a metal salt (e.g. divalent chromium salts such as chromous chloride, chromous acetate, etc.) or zinc or amalgamated zinc] in a suitable solvent [e.g.
- a metal hydride e.g. lithium aluminum hydride, sodium borohydride, tributyltin hydride, etc.
- alkali metal e.g. lithium, sodium, etc.
- a metal salt e.g. divalent chromium salts such as chromous chloride, chromous acetate, etc.
- zinc or amalgamated zinc e.g.
- the method is practiced at a temperature of from -50° C. to 150° C. and, for better results, at room temperature to 80° C. for 0.5 to 10 hours.
- the starting compound is dissolved in a suitable solvent and, then, the routine procedure is applied.
- a suitable solvent e.g. alcohol (e.g. methanol, ethanol, etc.), ammonia, dimethylformamide, etc.)] and the reduction is carried out using a low overvoltage electrode (e.g. platinum, wolfram, etc.) or a high overvoltage electrode (e.g. lead, zinc, mercury, etc.).
- a solvent e.g. alcohol (e.g. methanol, ethanol, etc.), ammonia, dimethylformamide, etc.)
- a low overvoltage electrode e.g. platinum, wolfram, etc.
- a high overvoltage electrode e.g. lead, zinc, mercury, etc.
- the reaction is carried out for one hour to 5 hours, at a temperature between about 20° and 100° C. preferably between about 60° and 80° C.
- P-23924 AR is referred to briefly as AR
- P-23924 BR as BR
- P-23924 CR as CR
- P-23924 DR as DR
- Solubility AR, BR, CR and DR are readily or easily soluble in dimethylsulfoxide, pyridine, ethyl acetate, chloroform, .[.dioxan.]. .Iadd.dioxon.Iaddend.; slightly soluble or insoluble in water, n-hexane, petroleum ether.
- FIGS. 19 through 22 The wavelengths (nm) and E 1 cm 1% values giving absorption peaks immediately after dissolution in the respective solvents are presented in Table 3.
- FIGS. 19 through 22 ---represents the values measured in methanol,------the values in 0.1N HCl-90% aqueous methanol,------the values measured in 0.1N NaOH-90% aqueous methanol.
- the IR spectra by the potassium bromide disk method are shown in FIGS. 23 to 26.
- the main wave numbers giving absorption maxima are as follows.
- 3400-3500 2960, 1670, 1630, 1600, 1490, 1455, 1420, 1380, 1330, 1290, 1230, 1220, 1150, 1110, 1080, 1020, 980, 920, 870, 820, 790, 705 See FIG. 24.
- Protocollagen prolyl hydroxylase inhibiting activity This inhibitory activity was assayed by the method of R. E. Rhoads [Methods in Enzymology XVII B, 306 (1971)] using a partially purified enzyme preparation derived from chick embryo in accordance with the methods of K. I. Kivirriko et al and J. Halme et al.
- P-23924A 100 to 200 mg/kg
- P-23924B 50 to 100 mg/kg
- P-23924C 100 to 200 mg/kg
- P-23924D more than 400 mg/kg
- P-23924E Ca. 50 mg/kg
- P-23924F 50 to 100 mg/kg.
- P-23924 has protocollagen prolyl hydroxylase inhibiting and/or selective collagen biosynthesis suppressant activities and is low toxicity, and is therefore of use as inhibitors of animal tissue fibrosis, biochemical reagents, and so forth.
- P-23924 is of use as a prophylactic/therapeutic agent against organ fibrosis in mammals (e.g. rabbit, rat, mouse, dog, cat, man, etc.) and can be administered to such animals for the prevention and treatment of pulmonary fibrosis, liver cirrhosis, nephrosclerosis, arteriosclerosis scleroderma, myelofibrosis, chronic arthritis, etc.
- mammals e.g. rabbit, rat, mouse, dog, cat, man, etc.
- the dose of P-23924 varies with the disease, condition, subject, route of administration, etc.
- about 0.2 to 20 mg/kg preferably about 2 to 20 mg/kg per day per the mammal is administered in a single dose to 3 divided doses.
- P-23924 can be orally or parenterally administered as such or in combination with a pharmaceutically acceptable carrier, excipient or diluent in such dosage forms as powders, granules, tablets, capsules, injections, etc.
- binders e.g. hydroxypropyl-cellulose, hydroxypropylmethyl-cellulose, macrogol, etc.
- disintegrators e.g. starch, carboxymethyl-cellulose calcium, etc.
- excipients e.g. lactose, starch, etc.
- lubricants e.g. magnesium stearate, talc, etc.
- isotonicating agents e.g. glucose, D-sorbitol, D-mannitorl, sodium chloride, etc.
- preservatives e.g. benzyl alcohol, chlorobutanol, methyl p-hydroxybenzoate, propyl p-phydroxybenzoate, etc.
- buffers e.g. phosphate buffer, sodium acetate buffer, etc.
- P-23924 can also be used as biochemical reagents, for example as an inhibitor of collagen biosynthesis in cultured animal cells or as a specific inhibitor of protocollagen prolyl hydroxylase activity.
- FIGS. 1 to 6 and 19 to 22 show ultraviolet and visible absorption spectra of Physiologically Active Substances P-23924 A, B, C, D, E, F, AR, BR, CR and DR respectively; FIGS. 7 to 12 and 23 to 26, infrared absorption spectra of said substances, respectively; and FIGS. 13 to 18 and 27 to 30, nuclear magnetic resonance spectra of said substances, respectively.
- a culture of Streptomyces sp. No. 23924 (IFO 14205, FERM BP-338) was used for inoculation of 400 ml of a liquid medium containing 2.0% glucose, 1.0% glycerin, 0.5% raw soybean meal, 0.5% corn steep liquor, 0.3% Polypepton (Daigo Nutritive Chemicals, Japan), 0.3% sodium chloride and 0.5% calcium carbonate in each of five 2-liter Sakaguchi flasks. Cultivation at 28° C. on a reciprocal shaker for 2 days gave about 2 liters of a culture broth. This culture broth was transferred to a 200-liter tank containing 100 liters of the same medium as above and cultivated at 28° C.
- the filtrate and washings were combined, adjusted to pH 3.0 with sulfuric acid and subjected to counter-current extraction in a Podbielniak Extractor Model 6150 (Podbielniak, U.S.A.) using 1/2 volume of ethyl acetate.
- the resulting extract about 430 liters, was reextracted with 1/2 volume of 1 % aqueous sodium hydrogen carbonate, whereby 215 liters of an extract was obtained.
- This extract was adjusted to pH 3.0 with sulfuric acid, extracted again with 1/2 volume of ethyl acetate, washed twice with 1/2 volume of water and concentrated under reduced pressure.
- the concentrate 400 ml, was passed through a silica gel (Merck, West Germany) column (4.6 ⁇ 65 cm), and after passage of 500 ml of ethyl acetate, elution was carried out with 3 liters of 1% oxalic acid-ethyl acetate.
- the active fractions about 3 liters, were pooled, washed with two 500-ml portions of water and concentrated to dryness under reduced pressure.
- the solid residue was dissolved in about 300 ml of dimethyl sulfoxide.
- the solution was diluted with 30 ml of water and introduced into a preparative liquid chromatograph (Waters Associates, U.S.A.), the Prep LC/System 500A fitted with a Prep PAK-500/C 18 column. Elution was carried out with 3 liters of methanol-water (1:1), 5 liters of methanol-water (3:2) and 2 liters of methanol in the order mentioned. The eluate was collected in 500-ml fractions. There were obtained fractions rich in P-23924A, C, D and E (Fractions 2-8, 3.5 liters) and fractions rich in P-23924B (Fractions 9-13; 2.5 liters).
- the P-23924 B-containing fractions yielded orange-yellow crystals (about 2.3 g) of P-23924B. These crystals were separated by filtration and the mother liquor was concentrated under reduced pressure to about 1 liter. The concentrate was extracted with three 1/3-volume portions of ethyl acetate and the combined extract was dried over 50 g of anhydrous sodium sulfate and concentrated to about 30 ml. To the concentrate was added 200 ml of n-hexane, whereupon a crude orange-yellow crystalline powder (about 7.1 g) of P-23924B was obtained. The crude powder was recrystallized from methanol to give about 3 g of crystals of P-23924B.
- fractions rich in P-23924A, C, D and E were concentrated to about 100 ml, chromatographed on a silica gel column (4.0 ⁇ 42 cm) and eluted with 2 liters of 1% oxalic acid-ethyl acetate. The eluate was collected in 100-ml fractions, whereby fractions rich in P-23924A and D (Fractions 2-7; 600 ml) and fractions rich in P-23924C and E (Fractions 8-10 700 ml) were recovered. The former fractions (600 ml) were washed with water and concentrated to dryness. The solid residue was dissolved in 50 ml of methanol.
- the solution was diluted with 50 ml of water and introduced into the same preparative liquid chromatograph as above. Elution was carried out with 5.5 liters of methanol-water (1:1). The eluate was collected in 500-ml fractions, and 3 fractions (1.5 liters) from Fractions 13 to 15 were combined and concentrated to about 700 ml under reduced pressure.
- the concentrate was extracted with three 200-ml portions of ethyl acetate and the combined extract was dehydrated over 30 g of anhydrous sodium sulfate and concentrated to about 30 ml under reduced pressure. To concentrate was added 200 ml of n-hexane, whereupon a crude orange-yellow powder (1.7 g) containing P-23924A and D was obtained.
- the crude powder was dissolved in 100 ml of chloroform-acetic acid (4:1) and chromatographed on a silica gel column (3.5 ⁇ 52 cm). Elution was carried out with 400 ml of chloroform-acetic acid (4:1) and 1 liter of chloroform-acetic acid (7:3). The eluate was collected in 10-ml fractions, whereby fractions rich in P-23924A (Fractions 25-29) and fractions rich in P-23924D (Fractions 36-136) were obtained. The former fractions (50 ml) were concentrated to dryness under reduced pressure and dissolved in 200 ml of methanol.
- the solution was diluted with 200 ml of water and again subjected to chromatography using the same preparative liquid chromatograph as above. Fractions rich in P-23924A were collected and concentrated under reduced pressure, extracted with ethyl acetate and, after dehydration, concentrated to give a crude powder (about 250 mg) of P-23924A. The crude powder was recrystalized from methanol to give orange-yellow crystals (about 100 mg) of P-23924A. The fractions (1 liter) rich in P-23924D were concentrated to dryness under reduced pressure and the solid residue was dissolved in about 500 ml of ethyl acetate. The solution was washed with three 200-ml portions of water.
- fractions rich in P-23924C and E (Fractions 8-14; 700 ml) collected by the above silica gel chromatography were washed with water and dehydrated, and the ethyl acetate was distilled off. The residue was dissolved in 50 ml of methanol. The solution was diluted 2 times with water and introduced into a preparative liquid chromatograph (the same column as above). Elution was carried out with methanol-water (1:1). The eluate was collected in 200-ml fractions. There were obtained fractions rich in P-23924E (Fractions 10-13; 800 ml) and fractions rich in P-23924C (Fractions 14-18; 1 liter).
- the fractions rich in P-23924E were concentrated under reduced pressure, extracted with three 1/3-volume portions of ethyl acetate and, after dehydration, concentrated to about 20 ml.
- To the concentrate was added 200 ml of n-hexane, whereupon a crude orange-yellow powder (about 120 mg) of P-23924E was obtained.
- the crude powder was dissolved in 30 ml of chloroform-methanol (3:2) and chromatographed on a silica gel column (3 ⁇ 45 cm). Elution was carried out with chloroform-methanol (3:2). The thus-obtained fractions rich in P-23924E were concentrated to 10 ml.
- the crude powder was dissolved in 70 ml of ethyl acetate, and the solution was washed with three 20-ml portions of water and dehydrated over 2.5 g of anhydrous sodium sulate.
- the ethyl acetate solution was concentrated to about 10 ml under reduced pressure.
- To the concentrate was added 25 ml of n-hexane, whereupon orange-yellow crystals (about 85 mg) of P-23924C were obtained.
- the concentrate was passed through a silica gel (Merck, West Germany) column (4.6 ⁇ 65 cm), and elution was carried out with 2 liters of ethyl acetate and then with 7 liters of ethyl acetate containing 1% oxalic acid.
- the active fractions, 2 liters, containing mainly P-23924F were collected, washed twice with 300 ml of water and then concentrated under reduced pressure to give solid residue.
- the solid residue was dissolved in 80 ml of chloroform-acetic acid (9:1).
- the solution was passed through a silica gel column (3.4 ⁇ 54 cm), and elution was carried out with 1.2 liter of chloroformacetic acid (9:1), 1.2 liters of chloroform-acetic acid (8:2) and 1.2 liters of chloroform-acetic acid (7:3) in that order.
- the active fractions, about 1.5 liters, containing mainly P-23924F were collected, concentrated under reduced pressure to give solid residue.
- the solid residue was dissolved in 400 ml of methanol-water (1:1) and then thus obtained solution was introduced into a preparative liquid chromatography, the Prep LC/System 500A fitted with a Prep PAK-500/C 18 column. Elution was carried out with 5 liters of methanol-water (3:2).
- Example 2 Sixty (60) liters of a seed culture obtained in the manner of Example 1 was transferred into a 2000-liter fermentation tank containing 1200 liters of a liquid medium composed of 4% soluble starch, 1% dextrin, 2% defatted soybean meal, 0.1% sodium thiosulfate, 0.05% ferrous sulfate heptahydrate, 0.05% dipotassium hydrogen phosphate and 0.03% potassium chloride (pH 6.0) and cultivated at 24° C. for 6 days under aeration and agitation.
- the culture broth (about 1100 liters) was filtered, extracted with ethyl acetate, reextracted with sodium hydrogen carbonate and again extracted with ethyl acetate in the manner of Example 1.
- This extract was concentrated to 700 ml.
- the concentrate was chromatographed on a silica gel column (5.4 ⁇ 65 cm). After passage of 3 liters of ethyl acetate, elution was carried out with 10 liters of 1% oxalic acid-ethyl acetate. Eluate fractions rich in P-23924A, B and D (2.6 liters) and fractions rich in P-23924C, E (4.2 liters) and F (3.0 liters) were recovered. The former fractions were washed with water and, after dehydration, the ethyl acetate was distilled off. The residue was dissolved in 200 ml of methanol.
- the concentrate was passed through a silica gel column (4.5 ⁇ 70 cm), elution being carried out with 1.5 liters of dichloromethane-acetic acid (9:1), 5.0 liters of dichloromethane-acetic acid (8:2) and 2 liters of dichloromethane-acetic acid (7:3) in that order.
- dichloromethane-acetic acid 9:1
- 5.0 liters of dichloromethane-acetic acid 8:2
- 2 liters of dichloromethane-acetic acid (7:3) in that order.
- the former fractions were washed with three 300-ml portions of water and the dichloromethane layer was concentrated to dryness.
- the solid residue was dissolved in 200 ml of methanol and diluted with 200 ml of water.
- the fractions rich in P-23924D (1.4 liters) recovered were concentrated to dryness under reduced pressure.
- the solid residue was dissolved in 200 ml of methanol and diluted with 200 ml of water.
- the dilution was subjected to preparative liquid chromatography under the same conditions as above and fractions rich in P-23924D (2.7 liters) were recovered.
- the methanol was distilled off under reduced pressure.
- the concentrate (1.5 liters) was extracted with three 1/3-volume portions of ethyl acetate. The extract was dehydrated and concentrated to about 25 ml. On standing, the concentrate yielded crystals of P-23924D (about 1 g).
- the P-23924 B-containing fractions (3.1 liters), on standing at room temperature, yielded crystals of P-23924B. These crystals were recovered and recrystallized from methanol to give crystals of P-2392B (about 3 g).
- the aforementioned P-23924C- and E-containing fractions (4.2 liters) was washed three times with 1 liter of water and dehydrated over 30 g of anydrous sodium sulfate, and the ethyl acetate was distilled off. The residue was dissolved in 200 ml of methanol and diluted with 200 ml of water.
- the dilution was subjected to liquid chromatography under the same conditions as above, and fractions rich in P-23924E (1.8 liters) and fractions rich in P-23924C (2.4 liters) were recovered.
- the P-23924E-containing fractions (1.8 liters) were concentrated to about 50 ml under reduced pressure.
- the concentrate was chromatographed on a Sephadex LH-20 (Pharmacia, Sweden) column (3.5 ⁇ 52 cm) (packed as a suspension in methanol) and eluted with methanol. Fractions containing P-23924E alone (200 ml) were combined and concentrated to 20 ml under reduced pressure.
- the active fractions, 1.8 liters, containing mainly P-23924F were collected and concentrated to dryness.
- the dried results was dissolved in 400 ml of methanol-water (1:1).
- methanol-water 1:1
- the active fractions, 2.4 liters, containing mainly P-23924F were collected, and concentrated, whereupon crude crystals of P-23924F were precipitated. Recrystallization of the crude crystals with methanol-water (3:2) gave 2.5 g of crystals of P-23924F.
- a mixture of the above ingredients is tableted by the conventional method (wet method).
- the above ingredients (1), (2), (3) and (4) (half the above amount) are mixed and granulated by the conventional method. To the granules is added the remaining amount of the ingredient (4). The mixture is packed into a gelatin capsule No. 1 (according to the Pharmacopoeia of Japan, Tenth Edition).
- the above ingredients (1), (2), (3) and (4) (half the above amount) are mixed and granulated by the conventional method. To the granules is added the remaining amount of the ingredient (4). The mixture is packed into a gelatin capsule No. 00 (according to the Pharmacopoeia of Japan, Tenth Edition).
- a mixture of the above ingredients is tableted by the conventional method (wet method).
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The compound of the formula: ##STR1## wherein R1 is hydrogen, methyl or hydroxymethyl, R2 is hydrogen or methoxy, R3 is hydroxy or methoxy, and R4 is hydrogen or a group ##STR2## is novel, and the compound is of use as inhibitors of animal tissue fibrosis, or biochemical reagents.
Description
The present invention relates to Physiologically Active Substance P-23924, its production and use.
Protocollagen prolyl hydroxylase is an enzyme which specifically hydroxylates the proline moiety of the protocollagen synthesized by ribosome in animal cells and is an important and rate-limiting factor in the biosynthesis of collagen. Among the compounds known to inhibit their enzyme activity are iron chelators (e.g. α,α'-dipyridyl etc.). SH enzyme inhibitors (e.g. p-chloromercury benzoate etc.) and certain heavy metals (e.g. Cu++, Zn++, etc.). However, because these substances invariably inhibit collagen biosynthesis non-specifically, they cause serious side effects and cannot be used as drugs. If a substance was found that would not inhibit biosynthesis of non-collagenous proteins but would specifically inhibit collagen biosynthesis, such substance could be used for the prevention and treatment of organ fibrosis accompanied by an excessive accumulation of collagen such as arteriosclerosis, liver cirrhosis, scleroderma, keloid, rheumatic arthritis, pulmonary fibrosis, etc.
In their search for a substance capable of inhibiting protocollagen prolyl hydroxylase activity among microbial metabolites, the present inventors discovered that an actinomycete strain produces Physiologically Active Substance P-23924A, B, C, D, E and F which are novel inhibitors of collagen biosynthesis and that these compounds are convertible to their reduced forms. This finding was followed by further research which has resulted in the perfection of the present invention.
The present invention relates to (1) a compound represented by the formula: ##STR3## wherein R1 is hydrogen, methyl or hydroxymethyl, R2 is hydrogen or methoxy, R3 is hydroxy or methoxy and R4 is hydrogen or a group ##STR4##
(2) a method of producing Physiologically Active Substance P-23924 A, B, C, D, E and/or F which comprises cultivating a microorganism belonging to the genus Streptomyces and capable of elaborating Physiologically Active Substance P-23924 A, B, C, D, E and/or F in a medium to cause said microorganism to elaborate and accumulate Physiologically Active Substance P-234924 A, B, C, D, E and/or F and recovering the same substance from the resulting culture broth.
(3) a method of producing a compound represented by the formula ##STR5## wherein R1 is hydrogen, methyl or hydroxymethyl, R2 is hydrogen or methoxy, and R3 is hydroxy or methoxy, which comprises subjecting to reduction a compound of the formula: ##STR6## wherein R1, R2 and R3 have the same meanings ad defined above, and
(4) an antifibriotic preparation containing Physiologically Active Substance P-23924.
The compounds of the compound (I) are named as follows:
__________________________________________________________________________ Name Abbreviation R.sub.1 R.sub.2 R.sub.3 R.sub.4 __________________________________________________________________________ Physiologically Active Substance P-23924A P-23924A CH.sub.3 H OCH.sub.3 ##STR7## Physiologically Active Substance P-23924B P-23924B CH.sub.3 OCH.sub.3 OCH.sub.3 ##STR8## Physiologically Active Substance P-23924C P-23924C H OCH.sub.3 OCH.sub.3 ##STR9## Physiologically Active Substance P-23924D P-23924D CH.sub.3 OCH.sub.3 OH ##STR10## Physiologically Active Substance P-23924E P-23924E CH.sub.2 OH H OCH.sub.3 ##STR11## Physiologically Active Substance P-23924F P-23924F CH.sub.2 OH OCH.sub.3 OCH.sub.3 ##STR12## Physiologically P-23924AR CH.sub.3 H OCH.sub.3 H Active Substance P-23924AR Physiologically P-23924BR CH.sub.3 OCH.sub.3 OCH.sub.3 H Active Substance P-23924BR Physiologically P-23924CR H OCH.sub.3 OCH.sub.3 H Active Substance P-23924CR Physiologically P-23924DR CH.sub.3 OCH.sub.3 OH H Active Substance P-23924DR __________________________________________________________________________
Moreover, the compounds of the formula (I) will sometimes be referred to generically or individually as Physiologically Active Substances P-23924 or briefly as P-23924.
The microorganism employed in accordance with the present invention may be any strain of microorganism that belongs to the genus Streptomyces and is able to elaborate Physilogically Active Substance P-23924 A, B, C, D, E and/or F (which will sometimes be referred to briefly as a P-23924 producing strain.
As an Example of the P-23924 producing strain, there may be mentioned Streptomyces sp. No. 23924 (hereinafter referred to briefly as Strain No. 23924) which was isolated from a soil sample collected in Ishigakijima, Okinawa Prefecture, Japan.
The characteristics of this Strain No. 23924 as determined in accordance with the methods described in International Journal of Systematic Bacteriology 16, No. 3, pp. 313-340 (1966) are as follows. Unless otherwise indicated, cultural characteristics were those found after 14 days incubation at 28° C.
Monopodially extending from vegetative mycelia are short sporulated filaments which are spiral or sometimes incompletely spiral, open-loop or hook-shaped, without whorl formation. Mature spore chains generally show 5 to 10 spores in chains. The spores are either cylindrical or elipsoidal and range in size from 0.4 to 0.7 by 0.7 to 1.2 microns, with smooth surfaces.
The degree of growth (G), growth and color of aerial mycelium (AM) and the production and color of soluble pigments (SP), among others, on various media are listed below. As to descriptions of colors, the standard color codes given in the Color Harmony Manual, Fourth Edition (1958) from Container Corporation of America were employed.
______________________________________ (a) Sucrose nitrate agar (G): Poor (AM): Sparse, light grayish brown (3 ge) (SP): None (b) Glucose asparagine agar (G): Poor (AM): None (SP): None (c) Glycerin asparagine agar (G): None (d) Starch inorganic salt agar (G): Good (AM): Abundant, powdery, (3 ih) (SP): Yellowish brown (4 gc) (e) Tyrosine agar (G): Poor (AM): Sparse, gray (3 ba) (SP): Light yellowish red (4 ea) (f) Nutrient agar (G): Moderate (AM): None (SP): None (g) Yeast malt agar (G): Moderate (AM): Sparse, gray (3 fe) (SP): Light yellowish brown (4 ne) (h) Oatmeal agar (G): Moderate (AM): Sparse, light grayish brown (3 ge) (SP): None (III) Physiological characteristics (a) Temperature range for growth: 15-35° C. (b) Liquefaction of gelatin (glucose peptone gelatin, 24° C., 3 weeks): positive (weak) (c) Hydrolysis of starch: positive (d) Coagulation and peptonization of skim milk: both negative (e) Reduction of nitrate: negative (f) Production of melanoid pigment: Tyrosine agar: suspected positive Peptone yeast iron agar: negative (IV) Assimilation of carbon sources (Pridham-Gottlieb agar) L-arabinose ± Inositol - D-xylose ++ L-rhamnose - D-glucose ++ Raffinose - D-fructose ± D-mannit - Sucrose ± Control - ______________________________________ (Note) ++: Good growth ±: Slight growth -: No growth
It is apparent from the above descriptions of characteristics that this particular strain belongs to the genus Streptomyces.
The above Streptomyces sp. No. 23924 strain has been deposited at Institute for Fermentation, Osaka (IFO), Japan under the accession number of IFO 14205 since Sept. 20, 1982, and this microorganism, which was deposited on Oct. 1, 1982 at Fermentation Research Institute Agency of Industrial science and Technology, Ministry of International Trade and Industry (FRI), Japan under the accession number of FERM P-6739, the deposit being converted to a deposit under the Budapest Treaty, has been stored at FRI under the accession number of FERM BP-338.
Microorganisms of the genus Streptomyces are generally liable to change in characteristics and can be easily caused to undergo mutation by artificial procedures such as X-ray, ultraviolet ray or radioactive ray irradiation or by a treatment employing a mutagenic agent. Such mutants, only if they are able to produce P-23924 A, B, C, D, E and/or F, can all be employed for the purposes of the present invention.
The medium for cultivation of P-23924 producing strains may be a liquid medium or a solid medium and it is generally advantageous to conduct shake culture or aerobic submerged culture using a liquid medium. The medium may be of any kind only if it is suitable for growth of Actinomyces and for the production thereby of P-23924A, B, C, D, E and/or F. Thus, as sources of carbon, there may be employed glucose, lactose, glycerin, starch, sucrose, dextrin, molasses, organic acids (e.g. acetic acid, tartaric acid, etc.) and so on. As sources of nitrogen, there may be employed peptone, Casamino acid (Difco, U.S.A.), N-Z Amine A (Sheffield, U.S.A.) and other protein hydrolysates, yeast extract, malt extract, soybean meal, corn steep liquor, amino acids (e.g. aspartic acid, glutamic acid, etc.), various ammonium salts (e.g. ammonium sulfate, ammonium chloride, etc.) and so on. It is also possible to add inorganic salts such as various phosphates (e.g. sodium dihydrogen phosphate, potassium monohydrogen phosphate, etc.), metal salts (e.g. magnesium sulfate, sodium chloride, ferrous sulfate, etc.), heavy metal salts (e.g. manganese sulfate, zinc sulfate, etc.) and so on. For the purpose of promoting growth of the strain, vitamins (e.g. vitamin B1, calcium pantothenate, etc.) nucleic acid related products (e.g. adenine, uracil, etc.), etc. may be added. Moreover, according to the cultural method and conditions used, the output of P-23924A, B, C, D, E and/or F may at times be increased by adding a defoaming agent such as silicone, polypropylene glycol ether derivative [e.g. Actocol (Takeda Chemical Industries, Ltd., Japan), etc.], soybean oil, etc. to the culture medium.
When the cultivation temperature and time, the pH of the medium, and other cultural conditions depend on the particular strain and the composition of the medium, they should be so selected and controlled that the output of P-23924 A, B, C, D, E and/or F will be maximal. It is in many cases desirable to conduct aerobic culture at about 20° to 40° C. for 24 to 240 hours while the pH of the medium is maintained at about 4 to 9. Recovery of Physiologically Active Substance P-23924 A, B, C, D, F and/or F from the culture broth can be easily accomplished by utilizing various procedures in a combination suited to the properties of the same substance. Among such procedure are extraction with an organic solvent which is neutral or weakly acidic and immiscible with water, such as ethyl acetate, butyl acetate, chloroform, butanol, benzene, toluene, diethyl ether, methylene chloride, methyl isobutyl ketone, etc., adsorption chromatography using activated carbon, silica gel, alumina, etc., gel filtration on a Sephadex column, ion exchange chromatography using ion exchange resins, and so on. By applying such procedures in a suitable combination, P-23924 A, B, C, D, E and/or F can be isolated as crystals or crystalline powders.
Various properties of Physiologically Active Substance P-23924 A, B, C, D, E and/or F as obtained in Example 2 which appears hereinafter are as follows.
(1) Physicochemical properties
(a) Melting point: A 186° to 188° C.; B 200° to 202° C.; C 187° to 190° C.; D 205° to 207° C.; E 174° to 176° C.; F 191° to 195° C.
(b) Elemental analysis (Found):
______________________________________ P-23924 C H N S ______________________________________ A 54.75 ± 1.0 4.92 ± 0.5 3.77 ± 0.5 8.09 ± 0.5 B 53.74 ± 1.0 4.99 ± 0.5 3.26 ± 0.5 7.29 ± 0.5 C 52.43 ± 1.0 4.72 ± 0.5 3.50 ± 0.5 7.82 ± 0.5 D 52.58 ± 1.0 4.61 ± 0.5 3.55 ± 0.5 7.84 ± 0.5 E 52.18 ± 1.0 4.58 ± 0.5 3.19 ± 0.5 7.55 ± 0.5 F 51.67 ± 1.0 4.75 ± 0.5 3.45 ± 0.5 7.30 ± 0.5 ______________________________________
(c) Molecular weight (based on mass spectrum) and molecular formula:
______________________________________ P-23924 Mol. wt. Mol. formula ______________________________________ A 393 C.sub.18 H.sub.19 NO.sub.7 S B 423 C.sub.19 H.sub.21 NO.sub.8 S C 409 C.sub.18 H.sub.19 NO.sub.8 S D 409 C.sub.18 H.sub.19 NO.sub.8 S E 409 C.sub.18 H.sub.19 NO.sub.8 S F 439 C.sub.19 H.sub.21 NO.sub.9 S ______________________________________
(d) Specific rotation:
______________________________________ P-23924 [α].sub.D .sup.23 (in methanol) C (%) ______________________________________ A -91 ± 10° 0.50 B -90 ± 10° 0.51 C -93 ± 10° 0.51 D -62 ± 10° 0.51 E -86 ± 10° 0.50 F -91 ± 10° 0.51 ______________________________________
(e) Solubility:
P-23924A, B, D and F are easily soluble in dimethyl sulfoxide, dimethylformamide, pyridine and 5% aqueous sodium hydrogen carbonate; soluble in methanol, dioxane and acetic acid; slightly soluble or insoluble in water, acetone, chloroform, n-hexane and petroleum ether. P-23924C is easily soluble in dimethyl sulfoxide, dimethylformamide, pyridine and 5% aqueous sodium hydrogen carbonate, soluble in methanol, dioxane, acetone and acetic acid; and slightly soluble or insoluble in water, chloroform, n-hexane and petroleum ether.
P-23924E is easily soluble in dimethyl sulfoxide, dimethylformamide, pyridine, 5% aqueous sodium hydrogen carbonate and methanol, soluble in dioxane and acetic acid, and slightly soluble or insoluble in water, chloroform; n-hexane and petroleum ether.
(f) Ultraviolet and visible absorption spectra:
See FIGS. 1 through 6. The wavelengths (nm) and E1 cm1% values giving absorption peaks immediately after dissolution in the respective solvents are presented in Table 1. In FIGS. 1 through 6,------represents the values measured in methanol,------the values in 0.1N HCl-90% aqueous methanol,------the values measured in 0.1N NaOH-90% aqueous methanol.
TABLE 1 __________________________________________________________________________ P-23924 Solvent Wave length (E.sub.lcm .sup.1%) Wave length (E.sub.lcm .sup.1%) Wave length (E.sub.lcm .sup.1%) __________________________________________________________________________ A B C Methanol 220 ± 2 (886 ± 90) 220 ± 2 (793 ± 80) .sup. 221 ± 2 (877 ± 90) 263 ± 2(sh) (421 ± 45) 262 ± 2(sh) (392 ± 40) .sup. 260 ± 2(sh) (398 ± 40) 270 ± 2 (458 ± 50) 268 ± 2 (428 ± 40) .sup. 265 ± 2 (415 ± 40) 420 ± 10 (129 ± 10) 308 ± 2 (214 ± 20) .sup. 306 ± 2 (256 ± 25) 420 ± 10 .sup. 420 ± 10 (108 ± 10) (124 ± 10) 0.1N HCl- 221 ± 2 (914 ± 90) 220 ± 2 (789 ± 80) .sup. 221 ± 2 (866 ± 90) 90% aq. 264 ± 2(sh) (442 ± 45) 261 ± 2(sh) (390 ± 40) .sup. 260 ± 2(sh) (403 ± 40) methanol 270 ± 2 (476 ± 50) 268 ± 2 (431 ± 45) .sup. 266 ± 2 (414 ± 40) 420 ± 10 (133 ± 15) 308 ± 2 (210 ± 20) .sup. 306 ± 2 (250 ± 25) 420 ± 10 (107 ± 10) .sup. 420 ± 10 (119 ± 10) 0.1N NaOH- 216 ± 2 (505 ± 50) 216 ± 2 (482 ± 50) .sup. 216 ± 2 (500 ± 50) 90% aq. 238 ± 2 (681 ± 70) 237 ± 2 (748 ± 75) .sup. 236 ± 2 (791 ± 80) methanol 277 ± 2 (248 ± 25) 284 ± 2 (215 ± 20) .sup. 286 ± 2 (213 ± 20) 540 ± 10 (131 ± 15) 540 ± 10 (128 ± 15) .sup. 540 ± 10 (142 ± 15) See FIG. 1 See FIG. 2 See FIG. 3 D E F Methanol 220 ± 2 (764 ± 80) 221 ± 2 (851 ± 90) 222 ± 2 (866 ± 90) 271 ± 2 (450 ± 45) 263 ± 2(sh) (393 ± 40) 262 ± 2(sh) (379 ± 40) 306 ± 2 (209 ± 20) 271 ± 2 (435 ± 45) 268 ± 2 (381 ± 40) 420 ± 10 (102 ± 10) 420 ± 10 (128 ± 10) 308 ± 2 (205 ± 20) 420 ± 15 (91 ± 10) 0.1N NCl- 220 ± 2 (762 ± 80) 221 ± 2 (869 ± 90) 221 ± 2 (852 ± 90) 90% aq. 271 ± 2 (456 ± 45) 264 ± 2(sh) (411 ± 40) 262 ± 2(sh) (374 ± 40) methanol 306 ± 2 (207 ± 20) 271 ± 2 (445 ± 50) 267 ± 2 (383 ± 40) 420 ± 10 (99 ± 10) 420 ± 10 (127 ± 15) 307 ± 2 (200 ± 20) 415 ± 15 (107 ± 10) 0.1N NaOH- 216 ± 2 (438 ± 45) 216 ± 2 (478 ± 50) 222 ± 2(sh) (548 ± 60) 90% aq. 234 ± 2 (576 ± 60) 238 ± 2 (633 ± 65) 236 ± 2 (730 ± 70) methanol 299 ± 2 (531 ± 55) 287 ± 2 (231 ± 25) 270 ± 2 (257 ± 30) 540 ± 20 (89 ± 10) 540 ± 10 (122 ± 15) 542 ± 20 (115 ± 10) See FIG. 4 See FIG. 5 See FIG. 6 __________________________________________________________________________ Sh = shoulder
______________________________________ (i) P-3300, 2930, 1710, 1665, 1640, 1620, 1570, 1540, 1500, 1460, 1420, 1380, 1335, 1280, 1240, 1200, 1180, 1110, 1040, 1000, 970, 900, 860, 800 See FIG. 7. (ii) P-23924B 3300, 2950, 1725, 1705, 1660, 1630, 1590, 1540, 1460, 1420, 1370, 1330, 1300, 1270, 1210, 1190, 1170, 1130, 1100, 1060, 1020, 980, 950, 890, 860, 790, 760, 700 See FIG. 8. (iii) P- 23924A 3400, 3070, 2940, 1720, 1680, 1635, 1600, 1540, 1490, 1460, 1420, 1380, 1350, 1310, 1250, 1220, 1180, 1120, 1100, 1040, 990, 930, 880, 820, 800, 705 See FIG. 9. (iv) P- 23924C 3400, 3300, 2960, 1720, 1670, 1635, 1610, 1540, 1440, 1380, 1340, 1300, 1260, 1220, 1205, 1130, 1100, 1060, 1000, 900, 870, 820, 800, 780, 690 See FIG. 10. (v) P- 23924D 23924E 3550, 3300, 3080, 2950, 1725, 1660, 1640, 1610, 1570, 1540, 1490, 1455, 1420, 1380, 1325, 1300, 1240, 1210, 1180, 1120, 1080, 1040, 1010, 940, 920, 860, 820, 800, 770, 700 See FIG. 11. (vi) P-3300, 1720, 1680, 1660, 1630, 1600, 1530, 1490, 1460, 1420, 1380, 1335, 1310, 1260, 1220, 1190, 1130, 1100, 1030, 1000, 980, 955, 870, 700 See FIG. 12. ______________________________________ 23924F
(h) Color reactions:
All species of P-23924 A, B, C, D, E and F give positive ferric chloride, Rydon-Smith, alcoholic magnesium acetate reactions, and negative ninhydrin and Ehrlich reactions.
(i) Description:
All species of P-23924 A, B, C, D, E and F are acidic and fat-soluble substances occurring as crystals, crystaline powders or powders which are yellow to yellow-orange or orange-yellow in color.
(j) Nuclear magnetic resonance spectra:
The NMR spectra determined in dimethyl-d6 sulfoxide at 400 MHz are shown in FIGS. 13 through 18. Characteristics signals are given below.
(i) P-23924A
δDMSO-d6 ppm: 1.86(3H, s), 2.09(3H, d, J=1.6 Hz). 2.71(1H, dd, J=8.8, 13.6 Hz), 2.91(1H, dd, J=5.0, 13.6 Hz), 3.66(1H, d, J=12.9 Hz), 3.78(1H, d, J=12.9 Hz), 3.96(3H, s), 4.47(1H, dt like, J=5.0, 8.3, 8.8 Hz), 6.89(1H, q, J=1.6 Hz), 7.09(1H, s), 8.18(1H, d, J=8.3 Hz), 12.40(1H, s) See FIG. 13.
(ii) P-23924B
δDMSO-d6 ppm: 1.86(3H, s), 1.95(3H, s), 2.70(1H, dd, J=8.8, 13.7 Hz), 2.89(1H, dd, J=4.9, 13.7 Hz), 3.65(1H, d, J=13.1 Hz), 3.77(1H, d, J=13.1 Hz), 3.95(3H, s), 4.03(3H, s), 4.47(1H, dt like, J=4.9, 8.1, 8.8 Hz), 7.11(1H, s), 8.18(1H, d, J=8.1 Hz), 12.59(1H, s) See FIG. 14.
(iii) P-23924C
δDMSO-d6 ppm: 1.85(3H, s), 2.71(1H, dd, J=8.8, 13.7 Hz), 2.91(1H, dd, J=4.9, 13.7 Hz), 3.66 (1H, d, J=12.9 Hz), 3.77(1H, d, J=12.9 Hz), 3.88 (3H, s), 3.95(3H, s), 4.47(1H, dt like, J=4.9, 8.1, 8.8 Hz), 6.26(1H, s), 7.15(1H, s), 8.18(1H, d, J=8.1 Hz), 12.73(1H, s) See FIG. 15.
(iv) P-23924D
δDMSO-d6 ppm: 1.85(3H, s), 1.94(3H, s), 2.73(1H, dd, J=8.8, 13.5 Hz), 2.92(1H, dd, J=4.8, 13.5 Hz), 3.64(1H, d, J=12.8 Hz), 3.76(1H, d, J=12.8 Hz), 4.00(3H, s), 4.48(1H, dt like, J=4.8, 8.1, 8.8 Hz), 7.03(1H, s), 8.17(1H, d, J=8.1 Hz), 12.79 (1H, s) See FIG. 16.
(v) P-23924E
δDMSO-d6 ppm: 1.85(3H, s), 2.71(1H, dd, J=8.5, 13.7 Hz), 2.90(1H, dd, J=4.9, 13.7 Hz), 3.67 (1H, d, J=13.1 Hz), 3.79(1H, d, J=13.1 Hz), 3.98 (3H, s), 4.47(2H, d, J=2.2 Hz), 4.47(1H, dt like, J=4.9, 8.1, 8.5 Hz), 5.47(1H, s), 6.82(1H, t, J=2.2 Hz), 7.15(1H, s), 8.18(1H, d, J=8.1 Hz), 12.29 (1H, s) See FIG. 17.
(vi) P-23924F
δDMSO-d6 ppm: 1.85(3H, s), 2.70(1H, dd, J=8.8, 13.7 Hz), 2.9(1H, dd, J=4.9, 13.7 Hz), 3.67(1H, H, d, J=13.1Hz), 3.80(1H, d, J=13.1 Hz), 3.97(3H, s), 4.10 (3H, s), 4.36(2H, d, J=3.7 Hz), 4.47(1H, dt like, J=4.9, 8.3, 8.8 Hz), 4.93(1H, br t), 7.16(1H, s), 8.20(1H, d, J=8.3 Hz), 12.70(1H, s) See FIG. 18.
(k) Rf values on thin layer chromatograms:
The Rf values on silica gel plates (Merck, Article No. 5729, West Germany) and reverse-phase HPTLC plates (RP-8, Merck, Article No. 13725, West Germany) are as follows.
______________________________________ P-23924 Solvent A B C D E F ______________________________________ (1) Silica gel plate, 0.39 0.42 0.30 0.19 0.12 0.22 chloroform-acetic acid (8:2) i-Propanol-acetic 0.45 0.48 0.29 0.57 0.45 0.39 acid (96:4) (2) Reverse-phase 0.61 0.55 0.69 0.60 0.75 0.72 plate, methanol- water (7:3) ______________________________________
Since there is not known any other compound having the above properties, P-23924 A, B, C, D, E and F are considered to be a novel substances.
Based on the above physicochemical properties, the following structural formula is proposed for P-23924 A, B, C, D, E and F.
______________________________________ ##STR13## Compound R.sub.1 R.sub.2 R.sub.3 ______________________________________ P-23924A CH.sub.3 H OCH.sub.3 P-23924B CH.sub.3 OCH.sub.3 OCH.sub.3 P-23924C H OCH.sub.3 OCH.sub.3 P-23924D CH.sub.3 OCH.sub.3 OH P-23924E CH.sub.2 OH H OCH.sub.3 P-23924F CH.sub.2 OH OCH.sub.3 OCH.sub.3 ______________________________________
Having a free carboxyl group, compound .[.(II).]. .Iadd.(III) is capable of forming salts. The conventional method can be used for producing such salts. The salts include, for example, salts with calcium, magnesium, barium sodium, potassium, manganese, iron, copper, zinc, etc.
The compound of the formula (I) wherein R4 is hydrogen [Compound (II)] is produced by means of reduction of the compound of the formula (I) wherein R4 is a group ##STR14##
[compound (III)].
The reduction is conducted by the manner per se known, such as catalytic reduction, electrolytic reduction and reduction using a reducing agent.
When the reduction is practiced by catalytic reduction, i.e. by reduction in the presence of a catalyst, the following procedure may be followed. Thus, the compound (III) is first dissolved in a routine solvent [e.g. alcohol (e.g. methanol, ethanol, isopropanol, etc.), dioxane, tetrahydrofuran, dimethylformamide or a mixture of such solvents[ and hydrogen gas is bubbled into the solution in the presence of a catalyst [e.g. Raney nickel, palladium on carbon, palladium barium carbonate, platinum oxide, rhodium complex, Raney type catalyst of cobalt, Raney type catalyst of iron, Raney type catalyst of copper]. This reaction is carried out at a temperature of 20° to 100° C., desirably at 60° to 80° C. for 10 minutes to 10 hours. While the reaction proceeds readily at atmospheric pressure, one may conduct it at elevated pressures between 5 to 200 kg/cm2.
When the reduction is to be practiced employing a reducing agent, the compound (III) is treated with a reducing agent [for example, a metal hydride (e.g. lithium aluminum hydride, sodium borohydride, tributyltin hydride, etc.), alkali metal (e.g. lithium, sodium, etc.), a metal salt (e.g. divalent chromium salts such as chromous chloride, chromous acetate, etc.) or zinc or amalgamated zinc] in a suitable solvent [e.g. methanol, ethanol, t-butanol, amylalcohol, dimethylformamide, dioxane, tetrahydrofuran, dimethylsulfoxide, ethylene glycol, ethylenediamine, diethylenetriamine, etc.]. The method is practiced at a temperature of from -50° C. to 150° C. and, for better results, at room temperature to 80° C. for 0.5 to 10 hours.
When electrolytic reduction is used, the starting compound is dissolved in a suitable solvent and, then, the routine procedure is applied. For example, the compound (III) is dissolved in a solvent [e.g. alcohol (e.g. methanol, ethanol, etc.), ammonia, dimethylformamide, etc.)] and the reduction is carried out using a low overvoltage electrode (e.g. platinum, wolfram, etc.) or a high overvoltage electrode (e.g. lead, zinc, mercury, etc.). Better results are sometimes obtained when the pH of the solution is brought to acidic, for example to pH 3 to 5.
The reaction is carried out for one hour to 5 hours, at a temperature between about 20° and 100° C. preferably between about 60° and 80° C.
The recovery of thus produced compound (II) can be carried out by the manner described as above.
The following is the physico-chemical properties of P-23924 AR, BR, CR and DR obtained in the Examples 3 to 6. In the following, P-23924 AR is referred to briefly as AR, P-23924 BR as BR, P-23924 CR as CR and P-23924 DR as DR.
(a) Melting point: AR 145° to 149° C.; BR 156° to 159° C.; CR 210° to 212° C.; DR 263° to 266° C.
(b) Elemental analysis (Found):
______________________________________ C H ______________________________________ AR 66.99 ± 1.0 5.27 ± 0.5 BR 63.93 ± 1.0 5.52 ± 0.5 CR 62.96 ± 1.0 5.10 ± 0.5 DR 63.03 ± 1.0 4.99 ± 0.5 ______________________________________
(c) Molecular weight (based on mass spectrum) and molecular formula:
______________________________________ Mol. wt. Mol. formula ______________________________________ AR 232 C.sub.13 H.sub.12 O.sub.4 BR 262 C.sub.14 H.sub.14 O.sub.5 CR 248 C.sub.13 H.sub.12 O.sub.5 DR 248 C.sub.13 H.sub.12 O.sub.5 ______________________________________
(d) Specific rotation:
______________________________________ [α].sub.D .sup.23 in dimethylsulfoxide C (%) ______________________________________ AR [0] 0° 0.20 BR [0] 0° 0.20 CR [0] 0° 0.20 DR [0] 0° 0.20 ______________________________________
(e) Solubility: AR, BR, CR and DR are readily or easily soluble in dimethylsulfoxide, pyridine, ethyl acetate, chloroform, .[.dioxan.]. .Iadd.dioxon.Iaddend.; slightly soluble or insoluble in water, n-hexane, petroleum ether.
(f) Ultraviolet and visible absorption spectra:
See FIGS. 19 through 22. The wavelengths (nm) and E1 cm 1% values giving absorption peaks immediately after dissolution in the respective solvents are presented in Table 3. In FIGS. 19 through 22,------represents the values measured in methanol,------the values in 0.1N HCl-90% aqueous methanol,------the values measured in 0.1N NaOH-90% aqueous methanol.
TABLE 3 __________________________________________________________________________ Wave length (E.sub.lcm .sup.1%) Solvent AR BR CR DR __________________________________________________________________________ Methanol 219 ± 2 (1374 ± 140) 218 ± 2 (1310 ± 130) 218 ± 2 (1417 ± 140) 218 ± 2 (1288 ± 130) 260 ± 2* (688 ± 70) 260 ± 2* (677 ± 70) 256 ± 2* (708 ± 70) 267 ± 2 (761 ± 80) 265 ± 2 (705 ± 70) 264 ± 2 (713 ± 70) 262 ± 2 (739 ± 70) 303 ± 2 (407 ± 40) 283 ± 2* (314 ± 30) 308 ± 2 (371 ± 40) 305 ± 2 (441 ± 40) 425 ± 10 (170 ± 20) 420 ± 10 (183 ± 20) 420 ± 10 (162 ± 20) 425 ± 10 (188 ± 20) 0.1N HCl- 219 ± 2 (1396 ± 140) 218 ± 2 (1294 ± 130) 218 ± 2 (1417 ± 140) 218 ± 2 (1314 ± 130) 90% aqueous 260 ± 2* (709 ± 70) 259 ± 2* (674 ± 70) 256 ± 2* (736 ± 70) 267 ± 2 (791 ± 80) methanol 266 ± 2 (728 ± 70) 264 ± 2 (722 ± 70) 263 ± 2 (771 ± 80) 306 ± 2 (391 ± 40) 283 ± 2* (333 ± 30) 308 ± 2 (365 ± 40) 307 ± 2 (448 ± 50) 425 ± 10 (174 ± 20) 420 ± 10 (188 ± 20) 425 ± 10 (165 ± 20) 425 ± 10 (195 ± 20) 0.1N NaOH- 234 ± 2 (1036 ± 100) 234 ± 2 (1111 ± 110) 233 ± 2 (1224 ± 120) 230 ± 2 (1038 ± 100) 90% aqueous 259 ± 10 (447 ± 50) 266 ± 2 (416 ± 40) 260 ± 2 (409 ± 40) 292 ± 2 (970 ± 100) methanol 283 ± 2* (391 ± 40) 295 ± 15* (294 ± 60) 285 ± 2 (343 ± 30) 440 ± 20 (149 ± 15) 425 ± 20 (99 ± 10) 420 ± 20 (79 ± 10) 305 ± 2* (329 ± 30) 535 ± 20 (143 ± 20) 535 ± 20 (181 ± 20) 530 ± 20 (166 ± 20) 535 ± 20 (203 ± 20) See FIG. 19 See FIG. 20 See FIG. 21 See FIG. 22 __________________________________________________________________________ *Shoulder
(g) Infrared absorption spectra:
The IR spectra by the potassium bromide disk method are shown in FIGS. 23 to 26. The main wave numbers giving absorption maxima are as follows.
(1) AR
3400-3450, 2900-3000, 1660, 1630, 1605, 1570, 1490, 1420, 1370, 1330, 1310, 1275, 1240, 1200, 1165, 1140, 1060, 1000, 910, 870, 810, 800, 700, 620 See FIG. 23.
(2) BR
3400-3500, 2960, 1670, 1630, 1600, 1490, 1455, 1420, 1380, 1330, 1290, 1230, 1220, 1150, 1110, 1080, 1020, 980, 920, 870, 820, 790, 705 See FIG. 24.
(3) CR
3400-3450, 2930, 1680, 1640, 1600, 1485, 1420, 1380, 1350, 1310, 1260, 1250, 1220, 1210, 1140, 1120, 1015, 980, 960, 920, 870, 850, 790, 710 See FIG. 25.
(4) DR
3430, 2950, 1655, 1620, 1595, 1490, 1450, 1430, 1360, 1310, 1290, 1220, 1130, 1100, 1080, 1020, 940, 890, 865, 830, 785, 760, 710, 685 See FIG. 26.
(h) Color reactions: AR, BR, CR and DR give positive ferric chloride, reduction with hydrosulfite; and negative ninhydrin and p-anisaldehyde-sulfuric acid reaction.
(i) Nuclear magnetic resonance spactra:
The NMR spectra determined in dimethyl-d6 sulfoxide at 400 MHz are shown in FIGS. 27 to 30. Characteristic signals are given below.
(1) AR
δDMSO-d6 ppm: 2.085(3H,d,J=1.6 Hz,3-CH3), 6.867 (1H,q,J=1.6 Hz,3-H), 7.057(1H,S,5-H), 3.952(3H,S,6-OCH3), 2.049(3H,S,7-CH3), 12.314(1H,S,disappeared on D2 O,8-OH) See FIG. 27
(2) BR
δDMSO-d6 ppm: 1.949(3H, S, 2-CH3), 4.026(3H, S, 3-OCH3), 7.100(1H, S, 5-H), 3.949(3H,S, 6-OCH3), 2.053(3H, S, 7-CH3), 12.510(1H, S, disappeared on D2 O, 8-OH) See FIG. 28.
(3) CR
δDMSO-d6 ppm: 6.242(1H, S, 2-H, S, 2-H), 3.870(3H, S, 3-OCH3), 7.127(1H, S, 5-H), 3.949(3H, S, 6-OCH3), 2.063(3H, S, 7-CH3), 12.631 (1H, S, disappeared on D2 O, 8-OH). See FIG. 29
(4) DR
δDMSO-d6 ppm: 1.918(3H, S, 2 or 7-CH3), 3.993(3H, S, 3-OCH3), 6.989(1H, S, 5-H), 10.940(1H, S, disappeared on D2 O, 6-OH), 2.005)3H, S 2 or 7 -CH3), 12.637(1H, S, disappeared on D2 O, 8-OH) See FIG. 30
(k) Rf values on thin layer chromatograms:
The Rf values on silica gel plates (Merck, Article No. 5729, West Germany) and reverse-phase HPTLC plates (RP-18, Merck, Article No. 13724) are as follows.
______________________________________ Solvent AR BR CR DR ______________________________________ (1) Silica gel plate, 0.65 0.72 0.29 0.35 ethyl acetate-n- hexane (1:5) Chloroform 0.82 0.83 0.50 0.15 (2) Reverse-phase plate, 0.54 0.50 0.61 0.69 methanol ______________________________________
Biological properties of P-23924 are as follows.
(a) Protocollagen prolyl hydroxylase inhibiting activity: This inhibitory activity was assayed by the method of R. E. Rhoads [Methods in Enzymology XVII B, 306 (1971)] using a partially purified enzyme preparation derived from chick embryo in accordance with the methods of K. I. Kivirriko et al and J. Halme et al. [Journal of Biological Chemistry 242, 4007 (1967) and Biochimica et Biophysica Acta 198, 460 (1967) and, as the substrate, (Pro-Pro-Gly)5,4H2 O [manufactured by Tanpakushitsu Kenkyu Shoreikai (Protein Research Foundation Society), Osaka, Japan]. The concentrations of P-23924 required for a 50% inhibition of 100 μg (as protein) of said partially purified enzyme preparation were as follows.
P-23924A: 6.7×10-5 M
P-23924B: 9.5×10-5 M
P-23924C: 5.7×10-5 M
P-23924D: 2.7×10-4 M
P-23924E: 2.1×10-5 M
P-23924F: 2.1×10-5 M
On the other hand, by a similar procedure as above, the protocollagen prolyl hydroxylase inhibiting activities on the compounds of P-23924 AR, BR, CR and DR are measured.
The results are shown in the following table.
______________________________________ Amount of Concentration the enzyme of the compound used Inhibition Compound (μM) (μg) (%) ______________________________________ P-23924 AR 12.5 100 26 3.13 20 37 P-23924 BR 12.5 100 22 3.13 20 26 P-23924 CR 12.5 100 33 3.13 20 40 P-23924 DR 12.5 100 20 3.13 20 33 ______________________________________
The above results indicate that P-23924 has strong inhibitory activity against protocollagen prolyl hydroxylase activity.
(b) Collagen biosynthesis suppressant activity: In accordance with the method of T, Ishimaru et al [Biochemical Pharmacology 31, 915 (1982)], P-23924 was intraperitoneally administered to SD rats (females, 3 weeks old) once daily for 6 consecutive days and the collagen content and non-collagenous protein content of the uterus were compared with those of control animals. As shown in Table 4, P-23924 caused a selective and significant suppression of collagen biosynthesis.
TABLE 4 __________________________________________________________________________ Total % Inhibition* Body protein Collagen Non- weight of content of content of collagenous Group rats (g) uterus (mg) uterus (mg) Collagen protein** __________________________________________________________________________ (A) Control (1) 75 ± 4 8.93 ± 0.70 1.71 ± 0.09 -- -- 5% ethanol-saline*** (B) Control (2) 72 ± 5 16.74 ± 1.97 2.94 ± 0.45 -- -- Estradiol-17β (5 μg) (C) Treatment Estradiol-17β (5 μg) + 73 ± 3 15.67 ± 1.63 2.53 ± 0.35 33 10 P-23924A (1 mg/kg) (P < 0.05) Estradiol-17β (5 μg) + 70 ± 5 14.92 ± 1.55 2.37 ± 0.43 46 19 P-23924B (1 mg/kg) (P < 0.02) Estradiol-17β (5 μg) + 73 ± 6 14.01 ± 1.51 2.19 ± 0.49 61 30 P-23924C (1 mg/kg) (P < 0.01) Estradiol-17β (5 μg) + 72 ± 3 15.63 ± 1.43 2.55 ± 0.30 32 11 P-23924D (50 mg/kg) (P < 0.05) Estradiol-17β (5 μg) + 72 ± 4 15.73 ± 1.59 2.52 ± 0.36 34 9 P-23924E (1 mg/kg) (P < 0.05) Estradiol-17β (5 μg) + 71 ± 3 15.20 ± 1.53 2.47 ± 0.57 38 15 P-23924F (1 mg/kg) (P < 0.05) __________________________________________________________________________ The marginal notes of the portion asterisked in the Table 4 are shown in below. ##STR15## **Noncollagenous protein = total protein content - collagen content ***Estradiol-17β was dissolved in 5% ethanol/physiological saline. I said tests, rats were used in groups of 10 individuals.
The acute toxicity [LC50 ] values of P-23924 in rats by the intraperitoneal route are given below.
P-23924A: 100 to 200 mg/kg,
P-23924B: 50 to 100 mg/kg,
P-23924C: 100 to 200 mg/kg,
P-23924D: more than 400 mg/kg,
P-23924E: Ca. 50 mg/kg,
P-23924F: 50 to 100 mg/kg.
Therefore, it is assumed that P-23924 is low toxicity.
As mentioned hereinbefore, P-23924 has protocollagen prolyl hydroxylase inhibiting and/or selective collagen biosynthesis suppressant activities and is low toxicity, and is therefore of use as inhibitors of animal tissue fibrosis, biochemical reagents, and so forth.
Thus, P-23924 is of use as a prophylactic/therapeutic agent against organ fibrosis in mammals (e.g. rabbit, rat, mouse, dog, cat, man, etc.) and can be administered to such animals for the prevention and treatment of pulmonary fibrosis, liver cirrhosis, nephrosclerosis, arteriosclerosis scleroderma, myelofibrosis, chronic arthritis, etc.
The dose of P-23924 varies with the disease, condition, subject, route of administration, etc. For the prevention/treatment of organ fibrosis, about 0.2 to 20 mg/kg preferably about 2 to 20 mg/kg per day per the mammal is administered in a single dose to 3 divided doses.
P-23924 can be orally or parenterally administered as such or in combination with a pharmaceutically acceptable carrier, excipient or diluent in such dosage forms as powders, granules, tablets, capsules, injections, etc.
In the production of oral preparations, there may be employed suitable amounts of binders (e.g. hydroxypropyl-cellulose, hydroxypropylmethyl-cellulose, macrogol, etc.), disintegrators (e.g. starch, carboxymethyl-cellulose calcium, etc.), excipients (e.g. lactose, starch, etc.), lubricants (e.g. magnesium stearate, talc, etc.) and so on.
In the production of parenteral or non-oral preparations, e.g. injections, there may be employed isotonicating agents (e.g. glucose, D-sorbitol, D-mannitorl, sodium chloride, etc.), preservatives (e.g. benzyl alcohol, chlorobutanol, methyl p-hydroxybenzoate, propyl p-phydroxybenzoate, etc.), buffers (e.g. phosphate buffer, sodium acetate buffer, etc.) and so on.
P-23924 can also be used as biochemical reagents, for example as an inhibitor of collagen biosynthesis in cultured animal cells or as a specific inhibitor of protocollagen prolyl hydroxylase activity.
FIGS. 1 to 6 and 19 to 22 show ultraviolet and visible absorption spectra of Physiologically Active Substances P-23924 A, B, C, D, E, F, AR, BR, CR and DR respectively; FIGS. 7 to 12 and 23 to 26, infrared absorption spectra of said substances, respectively; and FIGS. 13 to 18 and 27 to 30, nuclear magnetic resonance spectra of said substances, respectively.
The following examples are further illustrative of the present invention. In the description referring to culture media, all percents are weight/volume percents.
A culture of Streptomyces sp. No. 23924 (IFO 14205, FERM BP-338) was used for inoculation of 400 ml of a liquid medium containing 2.0% glucose, 1.0% glycerin, 0.5% raw soybean meal, 0.5% corn steep liquor, 0.3% Polypepton (Daigo Nutritive Chemicals, Japan), 0.3% sodium chloride and 0.5% calcium carbonate in each of five 2-liter Sakaguchi flasks. Cultivation at 28° C. on a reciprocal shaker for 2 days gave about 2 liters of a culture broth. This culture broth was transferred to a 200-liter tank containing 100 liters of the same medium as above and cultivated at 28° C. for 2 days under aeration and agitation. Sixty liters of the culture broth thus obtained was transferred to a 2000-liter fermentation tank containing 1200 liters of a liquid medium containing 4% soluble starch, 2% defatted soybean meal, 0.1% sodium thiosulfate, 0.05% magnesium sulfate, 0.05% dipotassium hydrogen phosphate and 0.03% potassium chloride (pH 6.0) and cultured at 24° C. for 5 days under aeration and agitation. To 1150 liters of the fermentation broth thus obtained was added 15 kg of Topco Perlite #34 (Toko Perlite Kogyo, Japan) and the mixture was filtered with a filter press precoated with 34 kg of Hyflo-Supercel (Johns-Manville Products, U.S.A.) to remove mycelia and solid materials. About 1000 liters of a filtrate was obtained. The mycelia and solid materials were washed with 150 liters of water and filtered again to give about 150 liters of the washings. The filtrate and washings were combined, adjusted to pH 3.0 with sulfuric acid and subjected to counter-current extraction in a Podbielniak Extractor Model 6150 (Podbielniak, U.S.A.) using 1/2 volume of ethyl acetate. The resulting extract, about 430 liters, was reextracted with 1/2 volume of 1 % aqueous sodium hydrogen carbonate, whereby 215 liters of an extract was obtained. This extract was adjusted to pH 3.0 with sulfuric acid, extracted again with 1/2 volume of ethyl acetate, washed twice with 1/2 volume of water and concentrated under reduced pressure. The concentrate, 400 ml, was passed through a silica gel (Merck, West Germany) column (4.6×65 cm), and after passage of 500 ml of ethyl acetate, elution was carried out with 3 liters of 1% oxalic acid-ethyl acetate. The active fractions, about 3 liters, were pooled, washed with two 500-ml portions of water and concentrated to dryness under reduced pressure. The solid residue was dissolved in about 300 ml of dimethyl sulfoxide. The solution was diluted with 30 ml of water and introduced into a preparative liquid chromatograph (Waters Associates, U.S.A.), the Prep LC/System 500A fitted with a Prep PAK-500/C18 column. Elution was carried out with 3 liters of methanol-water (1:1), 5 liters of methanol-water (3:2) and 2 liters of methanol in the order mentioned. The eluate was collected in 500-ml fractions. There were obtained fractions rich in P-23924A, C, D and E (Fractions 2-8, 3.5 liters) and fractions rich in P-23924B (Fractions 9-13; 2.5 liters). On standing at room temperature, the P-23924 B-containing fractions yielded orange-yellow crystals (about 2.3 g) of P-23924B. These crystals were separated by filtration and the mother liquor was concentrated under reduced pressure to about 1 liter. The concentrate was extracted with three 1/3-volume portions of ethyl acetate and the combined extract was dried over 50 g of anhydrous sodium sulfate and concentrated to about 30 ml. To the concentrate was added 200 ml of n-hexane, whereupon a crude orange-yellow crystalline powder (about 7.1 g) of P-23924B was obtained. The crude powder was recrystallized from methanol to give about 3 g of crystals of P-23924B. The fractions rich in P-23924A, C, D and E were concentrated to about 100 ml, chromatographed on a silica gel column (4.0×42 cm) and eluted with 2 liters of 1% oxalic acid-ethyl acetate. The eluate was collected in 100-ml fractions, whereby fractions rich in P-23924A and D (Fractions 2-7; 600 ml) and fractions rich in P-23924C and E (Fractions 8-10 700 ml) were recovered. The former fractions (600 ml) were washed with water and concentrated to dryness. The solid residue was dissolved in 50 ml of methanol. The solution was diluted with 50 ml of water and introduced into the same preparative liquid chromatograph as above. Elution was carried out with 5.5 liters of methanol-water (1:1). The eluate was collected in 500-ml fractions, and 3 fractions (1.5 liters) from Fractions 13 to 15 were combined and concentrated to about 700 ml under reduced pressure. The concentrate was extracted with three 200-ml portions of ethyl acetate and the combined extract was dehydrated over 30 g of anhydrous sodium sulfate and concentrated to about 30 ml under reduced pressure. To concentrate was added 200 ml of n-hexane, whereupon a crude orange-yellow powder (1.7 g) containing P-23924A and D was obtained. The crude powder was dissolved in 100 ml of chloroform-acetic acid (4:1) and chromatographed on a silica gel column (3.5×52 cm). Elution was carried out with 400 ml of chloroform-acetic acid (4:1) and 1 liter of chloroform-acetic acid (7:3). The eluate was collected in 10-ml fractions, whereby fractions rich in P-23924A (Fractions 25-29) and fractions rich in P-23924D (Fractions 36-136) were obtained. The former fractions (50 ml) were concentrated to dryness under reduced pressure and dissolved in 200 ml of methanol. The solution was diluted with 200 ml of water and again subjected to chromatography using the same preparative liquid chromatograph as above. Fractions rich in P-23924A were collected and concentrated under reduced pressure, extracted with ethyl acetate and, after dehydration, concentrated to give a crude powder (about 250 mg) of P-23924A. The crude powder was recrystalized from methanol to give orange-yellow crystals (about 100 mg) of P-23924A. The fractions (1 liter) rich in P-23924D were concentrated to dryness under reduced pressure and the solid residue was dissolved in about 500 ml of ethyl acetate. The solution was washed with three 200-ml portions of water. The ethyl acetate layer was dried over 50 g of anhydrous sodium sulfate and the solvent was distilled of under reduced pressure. To the resulting concentrate (about 30 ml) was added 200 ml of n-hexane, whereupon a crude powder (about 0.7 g) of P-23924D was obtained. The crude powder was dissolved in 25 ml of chloroform-acetic acid (4:1) and chromatographed on a silica gel column (3×45 cm). Elution was carried out with chloroform-acetic acid (4:1). The fractions with in P-23924D thus collected (400 ml) were concentrated to 80 ml under reduced pressure and the concentrate was diluted with 100 ml of water and extracted with three 1/3-volume portions of ethyl acetate. The combined extract was washed with water, dehydrated and deprived of the ethyl acetate. The thus-obtained crude powder (about 500 mg) was recrystallized from methanolacetic acid (1:5) to yield a crystalline orange-yellow powder (about 330 mg) of P-23924D. On the other hand, fractions rich in P-23924C and E (Fractions 8-14; 700 ml) collected by the above silica gel chromatography were washed with water and dehydrated, and the ethyl acetate was distilled off. The residue was dissolved in 50 ml of methanol. The solution was diluted 2 times with water and introduced into a preparative liquid chromatograph (the same column as above). Elution was carried out with methanol-water (1:1). The eluate was collected in 200-ml fractions. There were obtained fractions rich in P-23924E (Fractions 10-13; 800 ml) and fractions rich in P-23924C (Fractions 14-18; 1 liter). The fractions rich in P-23924E were concentrated under reduced pressure, extracted with three 1/3-volume portions of ethyl acetate and, after dehydration, concentrated to about 20 ml. To the concentrate was added 200 ml of n-hexane, whereupon a crude orange-yellow powder (about 120 mg) of P-23924E was obtained. The crude powder was dissolved in 30 ml of chloroform-methanol (3:2) and chromatographed on a silica gel column (3×45 cm). Elution was carried out with chloroform-methanol (3:2). The thus-obtained fractions rich in P-23924E were concentrated to 10 ml. To the concentrate were added 10 ml of ethyl acetate and 50 ml of n-hexane, whereupon a crystalline orange-yellow powder (about 30 mg) of P-23924E was obtained. The fractions (1 liter) rich in P-23924C were concentrated to dryness under reduced pressure and the solid residue was dissolved in 15 ml of chloroform and chromatographed on a silica gel column (3.0×45 cm). Elution was carried out with chloroform-acetic acid (4:1) to recover fractions rich in P-23924C. The fractions were concentrated to dryness, whereupon a crude powder (about 150 mg) of P-23924C was obtained. The crude powder was dissolved in 70 ml of ethyl acetate, and the solution was washed with three 20-ml portions of water and dehydrated over 2.5 g of anhydrous sodium sulate. The ethyl acetate solution was concentrated to about 10 ml under reduced pressure. To the concentrate was added 25 ml of n-hexane, whereupon orange-yellow crystals (about 85 mg) of P-23924C were obtained.
In the manner of the above, i.e. the procedures of extraction of the cultured broth with ethyl acetate, reextraction with 1% aqueous sodium hydrogen carbonate, extraction with ethyl acetate in acidic, washing with water and concentration, there was obtained 200 ml of a concentrate.
The concentrate was passed through a silica gel (Merck, West Germany) column (4.6×65 cm), and elution was carried out with 2 liters of ethyl acetate and then with 7 liters of ethyl acetate containing 1% oxalic acid. The active fractions, 2 liters, containing mainly P-23924F were collected, washed twice with 300 ml of water and then concentrated under reduced pressure to give solid residue. The solid residue was dissolved in 80 ml of chloroform-acetic acid (9:1). The solution was passed through a silica gel column (3.4×54 cm), and elution was carried out with 1.2 liter of chloroformacetic acid (9:1), 1.2 liters of chloroform-acetic acid (8:2) and 1.2 liters of chloroform-acetic acid (7:3) in that order. The active fractions, about 1.5 liters, containing mainly P-23924F were collected, concentrated under reduced pressure to give solid residue. The solid residue was dissolved in 400 ml of methanol-water (1:1) and then thus obtained solution was introduced into a preparative liquid chromatography, the Prep LC/System 500A fitted with a Prep PAK-500/C18 column. Elution was carried out with 5 liters of methanol-water (3:2). The fractions mainly containing P-23924F were collected, and concentrated under reduced pressure to give 1.3 g of crude powder of P-23924F. The crude powder was subjected to recrystallization with methanol-water (8:2), whereby about 500 mg of yellow-orange crystals of P-23924F was obtained.
Sixty (60) liters of a seed culture obtained in the manner of Example 1 was transferred into a 2000-liter fermentation tank containing 1200 liters of a liquid medium composed of 4% soluble starch, 1% dextrin, 2% defatted soybean meal, 0.1% sodium thiosulfate, 0.05% ferrous sulfate heptahydrate, 0.05% dipotassium hydrogen phosphate and 0.03% potassium chloride (pH 6.0) and cultivated at 24° C. for 6 days under aeration and agitation. The culture broth (about 1100 liters) was filtered, extracted with ethyl acetate, reextracted with sodium hydrogen carbonate and again extracted with ethyl acetate in the manner of Example 1. This extract was concentrated to 700 ml. The concentrate was chromatographed on a silica gel column (5.4×65 cm). After passage of 3 liters of ethyl acetate, elution was carried out with 10 liters of 1% oxalic acid-ethyl acetate. Eluate fractions rich in P-23924A, B and D (2.6 liters) and fractions rich in P-23924C, E (4.2 liters) and F (3.0 liters) were recovered. The former fractions were washed with water and, after dehydration, the ethyl acetate was distilled off. The residue was dissolved in 200 ml of methanol. To the resulting solution were added 200 ml of dimethyl sulfoxide and 200 ml of water. This solution was introduced into a preparative liquid chromatograph, the Prep LC/system 500A fitted with a Prep PAK-500/C18 column. Elution was carried out with methanol-water (1:1). Eluate fractions rich in P-23924A and D (2.5 liters) and fractions rich in P-23924B (3.1 liters) were thus recovered. The former fractions were again subjected to chromatography using the same liquid chromatograph as above. The fractions thus obtained were concentrated and extracted with ethyl acetate. This extract was dehydrated and concentrated to 50 ml. The concentrate was passed through a silica gel column (4.5×70 cm), elution being carried out with 1.5 liters of dichloromethane-acetic acid (9:1), 5.0 liters of dichloromethane-acetic acid (8:2) and 2 liters of dichloromethane-acetic acid (7:3) in that order. There were collected eluate fractions (1 liter) rich in P-23924A and fractions (1.2 liters) rich in P-23924D. The former fractions were washed with three 300-ml portions of water and the dichloromethane layer was concentrated to dryness. The solid residue was dissolved in 200 ml of methanol and diluted with 200 ml of water. The dilution was subjected to preparative liquid chromatography under the same conditions as above. Fractions rich in P-23924A were recovered, concentrated to remove the methanol and extracted with three 1/3-volume portions of ethyl acetate. The extracts were combined, dehydrated and concentrated to dryness. The residue was dissolved in methanol. On standing, the solution yielded crystals of P-23924A (300 mg). The P-23924D-containing solution (1.2 liters) was concentrated under reduced pressure. The concentrate, 30 ml, was chromatographed on a silica gel column (3.5×50 cm) and eluted with 1 liter each of dichloromethane-acetic acid mixtures (9:1, 8:2 and 7:3) in that order. The fractions rich in P-23924D (1.4 liters) recovered were concentrated to dryness under reduced pressure. The solid residue was dissolved in 200 ml of methanol and diluted with 200 ml of water. The dilution was subjected to preparative liquid chromatography under the same conditions as above and fractions rich in P-23924D (2.7 liters) were recovered. The methanol was distilled off under reduced pressure. The concentrate (1.5 liters) was extracted with three 1/3-volume portions of ethyl acetate. The extract was dehydrated and concentrated to about 25 ml. On standing, the concentrate yielded crystals of P-23924D (about 1 g). The P-23924 B-containing fractions (3.1 liters), on standing at room temperature, yielded crystals of P-23924B. These crystals were recovered and recrystallized from methanol to give crystals of P-2392B (about 3 g). The aforementioned P-23924C- and E-containing fractions (4.2 liters) was washed three times with 1 liter of water and dehydrated over 30 g of anydrous sodium sulfate, and the ethyl acetate was distilled off. The residue was dissolved in 200 ml of methanol and diluted with 200 ml of water. The dilution was subjected to liquid chromatography under the same conditions as above, and fractions rich in P-23924E (1.8 liters) and fractions rich in P-23924C (2.4 liters) were recovered. The P-23924E-containing fractions (1.8 liters) were concentrated to about 50 ml under reduced pressure. The concentrate was chromatographed on a Sephadex LH-20 (Pharmacia, Sweden) column (3.5×52 cm) (packed as a suspension in methanol) and eluted with methanol. Fractions containing P-23924E alone (200 ml) were combined and concentrated to 20 ml under reduced pressure. To the concentrate were added 20 ml of ethyl acetate and 200 ml of n-hexane, where-upon an orange-yellow crystalline powder of P-23924E (about 700 mg) was obtained. The P-23924C-containing fractions (2.4 liters) were concentrated under reduced pressure. The concentrate (1.2 liters) was extracted with three 1/3-volume portions of ethyl acetate, and the ethyl acetate layer was dehydrated over 20 g of anhydrous sodium sulfate and, then, the ethyl acetate was distilled off. The residue was dissolved in methanol. The resulting solution, on standing, yielded crude crystals of P-23924C. The crude crystals were recrystallized from methanol to give crystals of P-23924C (1.5 g).
The above active fractions, 3.0 liters, containing mainly P-23924F was washed with water and dried over. Ethyl acetate was evaporated, and thus obtained residue was dissolved in 100 ml of chloroform-acetic acid (9:1). The solution was passed through a silica gel column (3.4×54 cm), and elution was carried out with 1 liter of chloroform-acetic acid (9:1), 1 liter of chloroform-acetic acid (8.2) and 1 liter of chloroform-acetic acid (7:3).
The active fractions, 1.8 liters, containing mainly P-23924F were collected and concentrated to dryness. The dried results was dissolved in 400 ml of methanol-water (1:1). Thus obtained solution was introduced into a preparative liquid chlomatograph, the Prep LC/System 500A fitted with a Prep PAK-500C18 column. Elution was carried out with 3.0 liters of methanol-water (3:2). The active fractions, 2.4 liters, containing mainly P-23924F were collected, and concentrated, whereupon crude crystals of P-23924F were precipitated. Recrystallization of the crude crystals with methanol-water (3:2) gave 2.5 g of crystals of P-23924F.
In 300 ml .[.fo.]. .Iadd.of .Iaddend.methanol was dissolved 1 g of P-23924A, and the solution is subjected to catalytic reduction with 5 g of Raney nickel under the initial pressure of 100 Kg/cm2 and at reaction temperature 70° C. for 5 hours. Thus obtained reaction mixture was subjected to filtration to remove the catalyst, and the filtrate was concentrated to 20 ml under reduced pressure. The concentrate was diluted with water to give a 300 ml solution. The solution was extracted three times with 100 ml each portion of ethyl acetate, the extracts were combined and washed twice with 50 ml of water, the ethyl acetate layers were separated, dried and concentrated under reduced prressure to give 119 mg of orange red crystals of P-23924AR.
In 500 ml of methanol was dissolved 2.5 g of P-23924B, and the solution is subjected to catalytic reduction with 15 g of Raney nickel under the initial pressure of 100 Kg/cm2 and at the reaction temperature 70° C. for 5 hours. Thus obtained reaction mixture was subjected to filtration to remove the catalyst, and the filtrate was concentrated to dryness under reduced pressure. The residue was dissolved in 250 ml of ethyl acetate. The solution was washed three times with 100 ml each portion of water, the ethyl acetate layers were separated, dried and concentrated under reduced pressure to give whereby 0.9 g of orange red crystals of P-23924BR.
In 700 ml of methanol was dissolved 3 g of P-23924C, and the solution is subjected to catalytic reduction with 15 g of Raney nickel under the initial pressure of 100 Kg/cm2 and at reaction temperature 70° C. for 5 hours. Thus obtained reaction mixture was subjected to filtration to remove the catalyst, and the filtrate was concentrated under reduced pressure to dryness. The residue was dissolved in 800 ml of ethyl acetate. The solution was washed three times with 300 ml each portion of water, the ethyl acetate layers were separated, dried and concentrated under reduced pressure to give whereby 1.23 g of orange red crystals of P-23924CR.
One gram of P-23924 D was treated with the manner of Example 3, whereupon 341 mg of orange red cyrstals of P-23924 DR was obtained.
______________________________________ Tablet ______________________________________ (1) P-23924A, B, E orF 100 mg (2) Lactose 47 mg (3)Corn starch 40 mg (4) Hydroxypropylcellulose-L 12 mg (5)Magnesium stearate 1mg 200 mg/tablet ______________________________________
A mixture of the above ingredients is tableted by the conventional method (wet method).
______________________________________ Capsule ______________________________________ (1) P-23924C 100 mg (2) Lactose 135 mg (3)Corn starch 60 mg (4)Magnesium stearate 5mg 300 mg/capsule ______________________________________
The above ingredients (1), (2), (3) and (4) (half the above amount) are mixed and granulated by the conventional method. To the granules is added the remaining amount of the ingredient (4). The mixture is packed into a gelatin capsule No. 1 (according to the Pharmacopoeia of Japan, Tenth Edition).
______________________________________ Capsule ______________________________________ (1) P-23924D 300 mg (2) Lactose 135 mg (3)Corn starch 60 mg (4)Magnesium stearate 5mg 500 mg/capsule ______________________________________
The above ingredients (1), (2), (3) and (4) (half the above amount) are mixed and granulated by the conventional method. To the granules is added the remaining amount of the ingredient (4). The mixture is packed into a gelatin capsule No. 00 (according to the Pharmacopoeia of Japan, Tenth Edition).
______________________________________ Tablet ______________________________________ (1) P-23924 AR, BR, CR orDR 100 mg (2) Lactose 47 mg (3)Corn starch 40 mg (4) Hydroxypropylcellulose-L 12 mg (5)Magnesium stearate 1mg 200 mg/tablet ______________________________________
A mixture of the above ingredients is tableted by the conventional method (wet method).
Claims (14)
- .]. .[.2. A compound as claimed in claim 1, wherein R4 is a group ##STR18##
- .]. .[.3. A compound as claimed in claim 1, wherein R4 is hydrogen..]. .[.4. A compound as claimed in claim 1, wherein R1 is methyl, R2 is hydrogen, R3 is methoxy and R4 is a group ##STR19##
- .]. .[.5. A compound as claimed in claim 1, wherein R1 is methyl, R2 is methoxy, R3 is methoxy and R4 is a group ##STR20##
- .]. .[.6. A compound as claimed in claim 1, wherein R1 is hydrogen, R2 is methoxy, R3 is methoxy and R4 is a group ##STR21##
- .]. .[.7. A compound as claimed in claim 1, wherein R1 is methyl, R2 is methoxy, R3 is hydroxy and R4 is a group ##STR22##
- .]. .[.8. A compound as claimed in claim 1, wherein R1 is hydroxymethyl, R2 is hydrogen R3 is methoxy and R4 is a group ##STR23##
- .]. .[.9. A compound as claimed in claim 1, wherein R1 is hydroxymethyl, R2 is methoxy, R3 is methoxy and R4 is a group ##STR24##
- .]. .[.10. A compound as claimed in claim 1, wherein R1 is methyl, R2 is hydrogen, R3 is methoxy and R4 is hydrogen..]. .[.11. A compound as claimed in claim 1, wherein R1 is methyl, R2 is methoxy, R3 is methoxy and R4 is hydrogen..]. .[.12. A compound as claimed in claim 1, wherein R1 is hydrogen, R2 is methoxy, R3 is methoxy and R4 is hydrogen..]. .[.13. A compound as claimed in claim 1, wherein R1 is methyl, R2 is methoxy, R3 is hydroxy and R4 is hydrogen..]. .[.14. An antifibrotic preparation comprising an effective amount of at least one compound of claim 1 and a
- pharmaceutically acceptable carrier, excipient or diluent..]. .Iadd.15. A compound represented by the formula: ##STR25## wherein R1 is hydrogen, methyl or hydroxymethyl, R2 is hydrogen or methoxy, R3 is hydroxy or methoxy and R4 is a group ##STR26##
- .Iaddend. .Iadd.16. A compound as claimed in claim 15, wherein R1 is methyl, R2 is hydrogen and R3 is methoxy. .Iaddend. .Iadd.17. A compound as claimed in claim 15, wherein R1 is methyl, R2 is methoxy and R3 is methoxy. .Iaddend. .Iadd.18. A compound as claimed in claim 15, wherein R1 is hydrogen, R2 is methoxy and R3 is methoxy. .Iaddend. .Iadd.19. A compound as claimed in claim 15, wherein R1 is methyl, R2 is methoxy and R3 is hydroxy. .Iaddend. .Iadd.20. A compound as claimed in claim 15, wherein R1 is hydroxymethyl, R2 is hydrogen and R3 is methoxy. .Iaddend. .Iadd.21. A compound as claimed in claim 15, wherein R1 is hydroxymethyl, R2 is methoxy and R3 is methoxy. .Iaddend. .Iadd.22. A compound represented by the formula: ##STR27##
- .Iaddend. .Iadd.23. A compound represented by the formula: ##STR28##
- .Iaddend. .Iadd.24. A compound represented by the formula: ##STR29##
- .Iaddend. .Iadd.25. A compound represented by the formula: ##STR30##
- .Iaddend. .Iadd.26. An antifibrotic preparation comprising an effective amount of at least one compound of any one of claims 15 or 22-25, and a pharmaceutically acceptable carrier, excipient or diluent. .Iaddend.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/693,107 USRE34414E (en) | 1982-10-20 | 1991-04-22 | Physiologically active substance P-23924, its production and use |
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP18504382A JPS5974992A (en) | 1982-10-20 | 1982-10-20 | Physiologically active substance p-23924, its preparation and pharmaceutical |
JP57-185043 | 1982-10-20 | ||
JP58-142347 | 1983-08-02 | ||
JP58142347A JPS6034185A (en) | 1983-08-02 | 1983-08-02 | Physiologically active substance p-23924, its preparation, and pharmaceuitical preparation |
US06/543,943 US4569943A (en) | 1982-10-20 | 1983-10-19 | Physiologically active substance P-23924, its production and use |
US07/693,107 USRE34414E (en) | 1982-10-20 | 1991-04-22 | Physiologically active substance P-23924, its production and use |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US06/543,943 Reissue US4569943A (en) | 1982-10-20 | 1983-10-19 | Physiologically active substance P-23924, its production and use |
Publications (1)
Publication Number | Publication Date |
---|---|
USRE34414E true USRE34414E (en) | 1993-10-19 |
Family
ID=27472443
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US07/693,107 Expired - Fee Related USRE34414E (en) | 1982-10-20 | 1991-04-22 | Physiologically active substance P-23924, its production and use |
Country Status (1)
Country | Link |
---|---|
US (1) | USRE34414E (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5744623A (en) * | 1994-08-01 | 1998-04-28 | Pharma Mar, S.A. | Terpene-quinones with antitumour activity |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2523127A1 (en) * | 1982-03-11 | 1983-09-16 | Elmu Sa | N, S-DIACETYLCYSTEINE USEFUL AS A MUCOLYTIC AGENT, ITS MANUFACTURING PROCESS AND PHARMACEUTICAL COMPOSITIONS CONTAINING THE SAME |
GB2132643A (en) * | 1982-12-08 | 1984-07-11 | Oreal | Use of 5-hydroxynaphthoquinones for dyeing human keratin fibres |
-
1991
- 1991-04-22 US US07/693,107 patent/USRE34414E/en not_active Expired - Fee Related
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2523127A1 (en) * | 1982-03-11 | 1983-09-16 | Elmu Sa | N, S-DIACETYLCYSTEINE USEFUL AS A MUCOLYTIC AGENT, ITS MANUFACTURING PROCESS AND PHARMACEUTICAL COMPOSITIONS CONTAINING THE SAME |
GB2132643A (en) * | 1982-12-08 | 1984-07-11 | Oreal | Use of 5-hydroxynaphthoquinones for dyeing human keratin fibres |
Non-Patent Citations (6)
Title |
---|
Chemical Abstracts, vol. 101, No. 116,580x, Hocquaux, "5-Hydroxy-1,4-Naphthaquinone Dye for Keratin Fibers, Especially Hair," 1984. |
Chemical Abstracts, vol. 101, No. 116,580x, Hocquaux, 5 Hydroxy 1,4 Naphthaquinone Dye for Keratin Fibers, Especially Hair, 1984. * |
Chemical Abstracts, vol. 85, No. 46, 287g, Kurt, "Die-1-Alde Reaction of Thiophene Oxides Generated in Situ," 1976. |
Chemical Abstracts, vol. 85, No. 46, 287g, Kurt, Die 1 Alde Reaction of Thiophene Oxides Generated in Situ, 1976. * |
Chemical Abstracts, vol. 97, 1982, 127698s Brook et al., and original J. Chem. Res. Synop article cited therein. * |
Takara Shuzo et al., Chemical Abstracts, vol. 98, 1983, 124173b. * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5744623A (en) * | 1994-08-01 | 1998-04-28 | Pharma Mar, S.A. | Terpene-quinones with antitumour activity |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US4530835A (en) | CL-1577 Antibiotic compounds and their production | |
US5106864A (en) | Antitumor substance be-13793c | |
US4569943A (en) | Physiologically active substance P-23924, its production and use | |
EP1216237B1 (en) | Process for the preparation of mycophenolic acid and derivatives thereof | |
EP0318056A2 (en) | Novel antibiotics and process for preparing the same | |
US4487761A (en) | Dopamine β-hydroxylase inhibitors | |
US4954641A (en) | Novel antitumor antibiotic substance and a method for production thereof | |
US5322854A (en) | Reveromycin A, method for preparing the same, and antitumor agent and antifungal agent comprising the same | |
USRE34414E (en) | Physiologically active substance P-23924, its production and use | |
US6297043B1 (en) | Mumbaistatin, a process for it's production and its use as a pharmaceutical | |
US5695984A (en) | Process for the preparation of antibiotics 10381B | |
US4598145A (en) | Albacarcins V and M | |
US5079232A (en) | Compound ks-505 useful for improving cerebral function | |
US4401812A (en) | Anthracycline antibiotics | |
US5334613A (en) | Antibacterial substance BE-24566B | |
US6030951A (en) | Chrysomycin derivative compounds and use as antitumor agents | |
US4461831A (en) | Antitumor agents albacarcins V and M | |
US5217885A (en) | Antitumor substance BE-13793C | |
EP0284358B1 (en) | Novel anti-tumor compounds, method for the preparation thereof, and pharmaceutical preparations containing them | |
US4615975A (en) | Purified culture of Actinomadura verrucaspora subspecies veractimyces | |
US5098935A (en) | Carcinostatic or antitumor antibiotic, conagenin, and production and uses thereof | |
US5217882A (en) | Microbial transformation process for producing antihypertensive N2 -glucuronide products | |
US5061695A (en) | Antitumor substance be-12406 | |
DK167006B1 (en) | 5-HYDROXY-1,4-NAVTOQUINONES, PROCEDURES FOR PREPARING THEREOF AND ANTIFIBROTIC PREPARATION CONTAINING SUCH A COMPOUND | |
JPH0645568B2 (en) | Physiologically active substance P-23924, production method and preparation thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
LAPS | Lapse for failure to pay maintenance fees |