USPP7636P - Mushroom plant named Brown Hybrid X618 - Google Patents

Mushroom plant named Brown Hybrid X618 Download PDF

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Publication number
USPP7636P
USPP7636P US07/363,095 US36309589V US7636P US PP7636 P USPP7636 P US PP7636P US 36309589 V US36309589 V US 36309589V US 7636 P US7636 P US 7636P
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mushroom
verticillium
disease
varieties
bubbles
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US07/363,095
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Mark C. Spear
Rebecca Miller
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SYLVAN SPAWN LABORATORY Inc WEST HILLS INDUSTRIAL PARK KITTANNING PA 16201
Sylvan Spawn Laboratory Inc
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Sylvan Spawn Laboratory Inc
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Assigned to SYLVAN SPAWN LABORATORY, INC., WEST HILLS INDUSTRIAL PARK, KITTANNING, PA. 16201 reassignment SYLVAN SPAWN LABORATORY, INC., WEST HILLS INDUSTRIAL PARK, KITTANNING, PA. 16201 ASSIGNMENT OF ASSIGNORS INTEREST. Assignors: MILLER, REBECCA, SPEAR, MARK C.
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H15/00—Fungi; Lichens

Definitions

  • Verticillium disease also called dry bubble and Verticillium spot
  • fungus Verticillium fungicola Preuss
  • Hassebr. var. fungicola as described by Gams and Zaaryen, Neth. J. Pl. Path. 88:57-58 (1982).
  • Verticillium disease is controlled with hygienic cultural practices and/or fungicides.
  • the most effective commercial fungicide approved and labeled for such control is zinc ethylene bisdithiocarbamate known to the trade as ZinebTM.
  • ZinebTM zinc ethylene bisdithiocarbamate
  • the Environmental Protection Administration has suspended its approval for the registration of this material, and it can no longer be used in the United States. Without it, many mushroom farms using conventional mushroom varieties will most likely not be able to sustain the yield and quality levels which they need to remain economically viable.
  • the present invention is a new and distinct variety of mushroom characterized particularly by its greater resistance to Verticillium disease when compared to commercially available lines 56B and 381 and by its unique electrophoretic isozyme phenotype.
  • This novel and distinct variety of mushroom is identified as Brown Hybrid X618.
  • FIG. 1 illustrates the isozyme patterns used to characterize the claimed variety of mushroom identified as Brown Hybrid X618.
  • FIG. 2 illustrates the disease severity protocol used to evaluate resistance of Brown Hybrid X618 and presently commercially available varieties of mushrooms to Verticillium disease.
  • FIG. 3 illustrates the incidence of bubbles at various Verticillium disease infection levels of Brown Hybrid X618 as compared to commercially available line 56B.
  • the bubbles referred are growth on mushroom beds of hypertrophied mushroom primordia, i.e., "bubbles", where normal mushrooms should have grown.
  • FIG. 4 is a photograph showing two views of the X618 mushroom variety of the subject invention.
  • homokaryons are roughly analogous to sperm and egg. They are derived from parent varieties and must be combined to produce a hybrid.
  • Sylvan 381 is a commercially available, high-yielding white mushroom which is susceptible to Verticillium disease.
  • Somycel 56B is a brown mushroom which is available from The Pennsylvania State University (PSU) Mushroom Culture Collection. It is used commercially, is low-yielding and somewhat resistant to Verticillium disease.
  • the homokaryons (identified as 381-907 and 56B-4186) were typed by isozyme electrophoresis as taught by Royce and May in Mycologia, 74:93-102 (1982), the disclosure of which is incorporated herein by reference.
  • the hybridization of X618 was achieved by dual culture of the homokaryons on agar by well-known methods.
  • the genetic markers for these homokaryons, for the parent strains 381 and 56B and for X618 hybrid are set forth in Table 1. Table 1 indicates that X618 contains genetic material from each of the parent lines and is, therefore, a true hybrid of the parent varieties.
  • FIG. 1 depicts the isozyme patterns of X618 which are referred to in Table 1.
  • the X618 hybrid is genetically unique and its isozyme pattern is identifiable and distinct from that of any commercial or published variety.
  • X618 is a tan to brown mushroom. The coloration is even to scrobiculate. Chroma meter readings of the mature pileus range on the CIE (Commission Internationale de l'Eclairage) scale from Yxy of 43.83, 0.3777, 0.3660, to a Yzy of 34.49, 0.3811, 0.3640, readings which correspond to The Royal Horticultural Society Colour Charts selections near 159A and 172D.
  • the stipe is always bright white. Cap color tends to lighten later in the cropping cycle or when grown in absolute darkness. The tissue does not bruise or discolor upon injury.
  • the cap margin is incurved. If the mushroom is permitted to become over-mature the cap margin becomes straight or flaring and the partial veil remains attached to the stipe.
  • the pileus of X618 is 20-150 mm in diameter, planar to hemispherical and slightly umbillicate.
  • the cuticle is smooth appressed fibrillose and not viscid.
  • Gill trama are irregularly parallel and divergent at the tip. Gills are closely spaced and the basidia are clavate and mostly two-spored.
  • Pleurocystidia are absent.
  • Pileus tramal hyphae are 22-32 ⁇ m long and 11-14 ⁇ m wide.
  • Pileus cutical hyphae are 10-12 ⁇ m in diameter and 23-27 ⁇ m in length.
  • Spores are approximately 9 ⁇ m in length and 6 ⁇ m in diameter. They are ovate in shape and without a germ pore. Spore color is purple-brown to black.
  • the X618 has a richer flavor than current white commercial mushroom varieties and is more firm and crunchy.
  • the yield test results are summarized in Table 2.
  • the yield characteristics of X618 are compared with a group of commercial mushroom varieties identified in Table 2 which were selected as representing the majority of the genetic variability which is presently available in commercial mushroom varieties.
  • the class designation used is that described by Royce and May in Mycologia 74:93-102 (1982), the disclosure of which is incorporated herein by reference. Included in the comparisons are the parent strains of X618.
  • the comparisons were made at three levels of Verticillium pathogen exposure (0, 100 and 1000 Verticillium spores added per pot). As measured in grams of mushrooms produced per test unit over a seven day period, Table 2 indicates that the yields of X618 compare favorably with the other varieties at the 0 exposure level and are better at the 100 and 1000 exposure levels than the preexisting varieties of mushrooms.
  • Table 2 further displays the results of two tests for disease resistance.
  • the tests measure the occurrences of certain characteristic symptoms of Verticillium disease. These symptoms are the (1) appearance of necrotic lesions on the cap and stem of the mushrooms and (2) growth on mushroom beds of "bubbles"--hypertrophied mushroom primordia--where normal mushrooms should be.
  • the comparative measurements for the first test are called “disease severity ratings.” The ratings numbers correspond to proportions of total mushrooms in test crops which exhibit all symptoms.
  • FIG. 2 describes the severity rating scale.
  • the comparative measurement for the second test (indicated as "Bubbles") is the count of occurrences of the hypertrophied mushroom primordia in each production test unit at each pathogen exposure level. Both measures indicate that X618 has significantly higher resistance to Verticillium disease than do the comparison varieties of preexisting commercial varieties of mushrooms.
  • Table 3 also displays the results of two tests for disease resistance. The results are similar to these which are reported in Table 2, except that X618 was compared only to its Somycel 56B parent, and it was compared at additional Verticillium disease exposure levels. As in the Table 2 test, both measures (bubbles and disease severity ratings) indicate that X618 has significantly higher resistance to Verticillium disease than its somewhat disease resistant parent.
  • FIG. 3 displays the results of the bubbles measurement test reported in Table 3 in graphical form.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

A new and unique variety of brown mushroom is described. It exhibits (1) high production yields when compared to common commercial varieties, (2) excellent Verticillium disease resistance and (3) a distinct electrophoretic isozyme pattern.

Description

FIELD OF THE INVENTION
The present invention relates to a new and unique variety of mushroom plant of Agaricus bisporus (Lange) Imbach [=Agaricus brunnescens Peck].
BACKGROUND OF THE INVENTION
Due to the endogenous automictic nuclear behavior of the cultivated mushroom as described by Kerrigan in Evolution and Agaricus bisporus, Ph.D. disseration (1989), University of California (Santa Barbara), few new and unique varieties of cultivated mushroom have become commercially available. Those that have become available and that have been used commercially were developed primarily to achieve high production yields and a bright, white color. As a result, nearly all commercially produced mushrooms are genetically very similar as described by Royce and May in Mycologia 74:93-102 (1982).
A consequence of the genetic similarity of most commercially-produced mushrooms is that they are susceptible to diseases caused by common pathogens. The diseases can have a devastating impact on the yield and quality of a mushroom harvest. Verticillium disease, also called dry bubble and Verticillium spot, is one of the most troublesome of these diseases. It is caused by the fungus Verticillium fungicola (Preuss) Hassebr. var. fungicola as described by Gams and Zaaryen, Neth. J. Pl. Path. 88:57-58 (1982).
Typically, Verticillium disease is controlled with hygienic cultural practices and/or fungicides. Currently, the most effective commercial fungicide approved and labeled for such control is zinc ethylene bisdithiocarbamate known to the trade as Zineb™. However, the Environmental Protection Administration has suspended its approval for the registration of this material, and it can no longer be used in the United States. Without it, many mushroom farms using conventional mushroom varieties will most likely not be able to sustain the yield and quality levels which they need to remain economically viable.
Consequently, the incorporation of disease resistance into the genetic makeup of mushroom varieties represents an efficient, cost-effective and environmentally responsible solution to the problem, and a need for such a disease resistant mushroom exists.
SUMMARY OF THE INVENTION
The present invention is a new and distinct variety of mushroom characterized particularly by its greater resistance to Verticillium disease when compared to commercially available lines 56B and 381 and by its unique electrophoretic isozyme phenotype. This novel and distinct variety of mushroom is identified as Brown Hybrid X618.
BRIEF DESCRIPTION OF THE FIGURES
FIG. 1 illustrates the isozyme patterns used to characterize the claimed variety of mushroom identified as Brown Hybrid X618.
FIG. 2 illustrates the disease severity protocol used to evaluate resistance of Brown Hybrid X618 and presently commercially available varieties of mushrooms to Verticillium disease.
FIG. 3 illustrates the incidence of bubbles at various Verticillium disease infection levels of Brown Hybrid X618 as compared to commercially available line 56B. The bubbles referred are growth on mushroom beds of hypertrophied mushroom primordia, i.e., "bubbles", where normal mushrooms should have grown.
FIG. 4 is a photograph showing two views of the X618 mushroom variety of the subject invention.
DETAILED DESCRIPTION OF THE INVENTION
The present invention is a new and distinct variety of the commonly cultivated white mushroom Agaricus bisporus (Lange) Imbach [=Agaricus brunnescens Peck]. It is referred to by the variety name of X618 and was produced in the breeding program of Sylvan Spawn Laboratory, Inc., Kittanning, Pa.
In mushroom breeding, compatible breeding lines, called homokaryons, are roughly analogous to sperm and egg. They are derived from parent varieties and must be combined to produce a hybrid.
The X618 variety resulted from the hybridization of two compatible homokaryons. The varieties from which these homokaryons were derived are called the parent varieties. These are Sylvan 381 and Somycel 56B. Sylvan 381 is a commercially available, high-yielding white mushroom which is susceptible to Verticillium disease. Somycel 56B is a brown mushroom which is available from The Pennsylvania State University (PSU) Mushroom Culture Collection. It is used commercially, is low-yielding and somewhat resistant to Verticillium disease.
The homokaryons (identified as 381-907 and 56B-4186) were typed by isozyme electrophoresis as taught by Royce and May in Mycologia, 74:93-102 (1982), the disclosure of which is incorporated herein by reference. The hybridization of X618 was achieved by dual culture of the homokaryons on agar by well-known methods. The genetic markers for these homokaryons, for the parent strains 381 and 56B and for X618 hybrid are set forth in Table 1. Table 1 indicates that X618 contains genetic material from each of the parent lines and is, therefore, a true hybrid of the parent varieties. FIG. 1 depicts the isozyme patterns of X618 which are referred to in Table 1. The X618 hybrid is genetically unique and its isozyme pattern is identifiable and distinct from that of any commercial or published variety.
X618 is a tan to brown mushroom. The coloration is even to scrobiculate. Chroma meter readings of the mature pileus range on the CIE (Commission Internationale de l'Eclairage) scale from Yxy of 43.83, 0.3777, 0.3660, to a Yzy of 34.49, 0.3811, 0.3640, readings which correspond to The Royal Horticultural Society Colour Charts selections near 159A and 172D. The stipe is always bright white. Cap color tends to lighten later in the cropping cycle or when grown in absolute darkness. The tissue does not bruise or discolor upon injury. The cap margin is incurved. If the mushroom is permitted to become over-mature the cap margin becomes straight or flaring and the partial veil remains attached to the stipe.
The pileus of X618 is 20-150 mm in diameter, planar to hemispherical and slightly umbillicate. The cuticle is smooth appressed fibrillose and not viscid. Gill trama are irregularly parallel and divergent at the tip. Gills are closely spaced and the basidia are clavate and mostly two-spored. Pleurocystidia are absent. Pileus tramal hyphae are 22-32 μm long and 11-14 μm wide. Pileus cutical hyphae are 10-12 μm in diameter and 23-27 μm in length. Spores are approximately 9 μm in length and 6 μm in diameter. They are ovate in shape and without a germ pore. Spore color is purple-brown to black.
The X618 has a richer flavor than current white commercial mushroom varieties and is more firm and crunchy.
Evaluations of X618 were conducted to assess its production yield potential and its resistance to Verticillium. Small scale production tests were performed in Sylvan Spawn Laboratory, Inc.'s mushroom cultivation facilities at Cabot, Pa. Larger scale tests were performed in the facilities of Moonlight Mushrooms, Inc. at Worthington, Pa.
After the new variety was established, it was reproduced and maintained asexually by standard techniques in the Sylvan Spawn Laboratories facilities at Cabot and Kittanning, Pa. using standard agar and grain media.
The yield test results are summarized in Table 2. The yield characteristics of X618 are compared with a group of commercial mushroom varieties identified in Table 2 which were selected as representing the majority of the genetic variability which is presently available in commercial mushroom varieties. The class designation used is that described by Royce and May in Mycologia 74:93-102 (1982), the disclosure of which is incorporated herein by reference. Included in the comparisons are the parent strains of X618. The comparisons were made at three levels of Verticillium pathogen exposure (0, 100 and 1000 Verticillium spores added per pot). As measured in grams of mushrooms produced per test unit over a seven day period, Table 2 indicates that the yields of X618 compare favorably with the other varieties at the 0 exposure level and are better at the 100 and 1000 exposure levels than the preexisting varieties of mushrooms.
Table 2 further displays the results of two tests for disease resistance. The tests measure the occurrences of certain characteristic symptoms of Verticillium disease. These symptoms are the (1) appearance of necrotic lesions on the cap and stem of the mushrooms and (2) growth on mushroom beds of "bubbles"--hypertrophied mushroom primordia--where normal mushrooms should be. The comparative measurements for the first test (indicated as "Disease") are called "disease severity ratings." The ratings numbers correspond to proportions of total mushrooms in test crops which exhibit all symptoms. FIG. 2 describes the severity rating scale. The comparative measurement for the second test (indicated as "Bubbles") is the count of occurrences of the hypertrophied mushroom primordia in each production test unit at each pathogen exposure level. Both measures indicate that X618 has significantly higher resistance to Verticillium disease than do the comparison varieties of preexisting commercial varieties of mushrooms.
Table 3 also displays the results of two tests for disease resistance. The results are similar to these which are reported in Table 2, except that X618 was compared only to its Somycel 56B parent, and it was compared at additional Verticillium disease exposure levels. As in the Table 2 test, both measures (bubbles and disease severity ratings) indicate that X618 has significantly higher resistance to Verticillium disease than its somewhat disease resistant parent. FIG. 3 displays the results of the bubbles measurement test reported in Table 3 in graphical form.
              TABLE 1                                                     
______________________________________                                    
Phenotypes of Alleles Found In                                            
X618, Its Parent Homokaryons and Its Parent Varieties                     
BIOCHEMICAL LOCI                                                          
Variety                                                                   
       AAT    ADH      EST  GPT    Pep-2  PGM                             
______________________________________                                    
381    100/   100/149  100/ 100/100                                       
                                   100/111                                
                                          100/181                         
       100             100                                                
56B     81/   149/149   82/ 100/133                                       
                                   100/111                                
                                          100/181                         
       100             82                                                 
381-907                                                                   
       100    149      100  100    111    100                             
56B-4186                                                                  
        81    149       82  133    111    181                             
X618    81/   149/149   82/ 100/133                                       
                                   111/111                                
                                          100/181                         
       100             100                                                
______________________________________                                    

              TABLE 2                                                     
______________________________________                                    
Mushroom Yields in Grams, Disease Severity Ratings and                    
Incidence of Bubbles For Various Mushroom Varieties                       
At Various Disease Infection Levels                                       
______________________________________                                    
VARIETIES                                                                 
       X618     S100      S110                                            
Variety                                                                   
       Hybrid   Hybrid    Hybrid  Mc310 Mc266                             
Type   Brown    Off White Off White                                       
                                  White White                             
Class  None     11        11      2     22                                
From   Sylvan   Sylvan    Sylvan  PSU   PSU                               
______________________________________                                    
0 Verticillium spores added per pot                                       
Yield  277      295       163     132   313                               
Severity                                                                  
       0        0         0       0     0                                 
Rating                                                                    
Bubbles                                                                   
       0        1         0       0     0                                 
100 Verticillium spores added per pot                                     
Yield  363      236       158     86    181                               
Severity                                                                  
       1        3         3       3     4                                 
Rating                                                                    
Bubbles                                                                   
       0        3          50+    33    47                                
1000 Verticillium spores added per pot                                    
Yield  336      66        227     54    299                               
Severity                                                                  
       2        5         5       5     5                                 
Rating                                                                    
Bubbles                                                                   
       7         50+       50+    50    98                                
______________________________________                                    
             VARIETIES                                                    
               S8132    S671           S381                               
        Variety                                                           
               Off     Golden   56B    Hybrid                             
        Type   White   White    Brown  White                              
        Class  31      19       27     11                                 
        From   Sylvan  Sylvan   Somycel                                   
                                       Sylvan                             
______________________________________                                    
        0 Verticillium spores adder per pot                               
        Yield  168     227      127    267                                
        Severity                                                          
               0       0        0      0                                  
        Rating                                                            
        Bubbles                                                           
               0       0        0      0                                  
        100 Verticillium spores added per pot                             
        Yield  217     145      136    349                                
        Severity                                                          
               3       3        3      4                                  
        Rating                                                            
        Bubbles                                                           
                77+    65       32     30                                 
        1000 Verticillium spores added per pot                            
        Yield  190     77       136    308                                
        Severity                                                          
               5       5        4      5                                  
        Rating                                                            
        Bubbles                                                           
               132     76       35      50+                               
______________________________________                                    

              TABLE 3                                                     
______________________________________                                    
Incidence of Bubbles For Variety 56B and X618                             
At Various Disease Infection Levels                                       
       VARIETY                                                            
Number of                                                                 
         56B             X618                                             
Verticillium       Severity          Severity                             
Spores   Bubbles   Rating    Bubbles Rating                               
______________________________________                                    
  0       0        0         0       0                                    
 100     37        4         0       0                                    
 500     41        4         0       2                                    
1000     50        4         4       3                                    
4000     96        5         7       3                                    
______________________________________

Claims (1)

What is claimed is:
1. A new and distinct variety of mushroom substantially as shown and described, characterized particularly as to novelty by its greater resistance to Verticillium disease when compared to commercially available lines 56B and 381 and by its unique electrophoretic isozyme phenotype.
US07/363,095 1989-06-08 1989-06-08 Mushroom plant named Brown Hybrid X618 Expired - Lifetime USPP7636P (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5304721A (en) * 1992-06-18 1994-04-19 Sylvan Spawn Laboratory Incorporated Method for the production of high proportions of homokaryons in breeding stock of the mushroom Agaricus bisporus
WO1998021975A1 (en) 1995-02-09 1998-05-28 Amycel, Inc. Mushroom caps with reduced scaling
US20070033679A1 (en) * 2005-08-04 2007-02-08 Amycel, Inc. Brown mushrooms for commercial production

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4608775A (en) * 1983-07-29 1986-09-02 National Research Development Corporation Mushroom mutant strains

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4608775A (en) * 1983-07-29 1986-09-02 National Research Development Corporation Mushroom mutant strains

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5304721A (en) * 1992-06-18 1994-04-19 Sylvan Spawn Laboratory Incorporated Method for the production of high proportions of homokaryons in breeding stock of the mushroom Agaricus bisporus
US5684228A (en) * 1992-06-18 1997-11-04 Sylvan Spawn Laboratory Incorporated Method for the production of high proportions of homokaryons in breeding stock of the mushroom agaricus bisporus
WO1998021975A1 (en) 1995-02-09 1998-05-28 Amycel, Inc. Mushroom caps with reduced scaling
US20070033679A1 (en) * 2005-08-04 2007-02-08 Amycel, Inc. Brown mushrooms for commercial production
WO2007019306A3 (en) * 2005-08-04 2009-04-30 Amycel Inc Brown mushrooms for commercial production
US7608760B2 (en) * 2005-08-04 2009-10-27 Amycel Inc. Brown mushrooms for commercial production
US20100212042A1 (en) * 2005-08-04 2010-08-19 Amycel, Inc. Strain of Brown Mushrooms for Commercial Production
AU2006278465B2 (en) * 2005-08-04 2011-06-02 Amycel, Inc. Brown mushrooms for commercial production
EP1993350B1 (en) 2005-08-04 2016-06-29 Amycel, Inc. Brown mushrooms for commercial production

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Owner name: SYLVAN SPAWN LABORATORY, INC., WEST HILLS INDUSTRI

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNORS:SPEAR, MARK C.;MILLER, REBECCA;REEL/FRAME:005088/0437

Effective date: 19890606