USPP6220P - Geranium plant "Cassandra" - Google Patents

Geranium plant "Cassandra" Download PDF

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USPP6220P
USPP6220P US06/882,119 US88211986V US6220P US PP6220 P USPP6220 P US PP6220P US 88211986 V US88211986 V US 88211986V US 6220 P US6220 P US 6220P
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cultivar
geranium
color
kaempferol
florets
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US06/882,119
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Richard Craig
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RESEARCH Corp A NEW YORK NOT-FOR-PROFIT CORP
Research Corp
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Research Corp
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Assigned to RESEARCH CORPORATION, A NEW YORK NOT-FOR-PROFIT CORP. reassignment RESEARCH CORPORATION, A NEW YORK NOT-FOR-PROFIT CORP. ASSIGNMENT OF ASSIGNORS INTEREST. Assignors: CRAIG, RICHARD
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  • the present invention relates to a new and distinct cultivar of geranium Pelargonium ⁇ hortorum called "Cassandra".
  • the cultivar is particularly well adapted to both commercial greenhouse production and garden performance.
  • the novel characteristics of the cultivar are more fully double florets, lighter floral color, and more compact plant habit when compared to the cultivar geranium "Veronica”.
  • the cultivar is further characterized by unique biochemical fingerprint profiles.
  • the cultivar was developed from an organized, scientifically designed breeding program carried out at the Department of Horticulture, The Pennsylvania State University, University Park, PA 16802 and specifically resulted from selection from the self-pollinated progeny of the geranium cultivar "Honseler's Glorie Lila", which is probably an asexual selection from "Purpurball”. The cultivar was asexually propagated by cuttings and the reproductions ran true.
  • FIG. 1 illustrates in color the cultivar including foliage and flowers.
  • FIG. 2 illustrates the anthocyanin profile obtained from HPLC. Quantitative values are found in the tables. Analyses included a single peak that represented both pelargonidin and petunidin 3,5-diglucosides. Corrections were made in accompanying tables.
  • FIG. 3 illustrates the flavonol profile obtained from HPLC. Quantitative values are found in the tables.
  • test plant was grown in full sun under glass, 60° F. night and 75° F. sunny days. Soilless medium was fertilized constantly with 300 ppm N-K. Color readings were taken under cool white fluorescent lamps at 220 foot-candles and color identification was by reference to The Royal Horticultural Society Colour Charts except where common terms of color definition are employed.
  • Form Semi-dwarf, free basal branching; comparatively compact growth; flowers relatively close to foliage; freer flowering; earlier flowering; significantly improved form in this color.
  • Petioles Fan 3, green group, 143-C (R.H.S.C.C.).
  • Shape Slight crook to petiole just below bud; hemispherical.
  • Blooming habit Continuous, upright, double, non-shattering hemispherical.
  • Peduncle Arises from node; opposed to leaf petiole.
  • Ovaries 1: green, very pubescent; 5- or 6- lobed, 5.0 mm in length.
  • Flavonoid extraction The sample size for flavonoid identification consisted of the petals from six florets just after anthesis. Three different samples were collected from each cultivar and handled separately for analysis. The petals were weighed, ground in 20 ml of 1% HCl-MeOH with a mortar and pestle, filtered through one layer of Whatman #1 filter paper, and washed with 1% HCl-MeOH. The volume was adjusted to 90 ml and 2-15 ml aliquots were removed for the analysis and handled separately. Each aliquot was taken to dryness at 40° C. in vacuo. All traces of HCl were removed by azeotropic distillation with MeOH.
  • One of the dried extracts was reconstituted in 2 ml of 1% HCl-MeOH and was used for anthocyanin analysis.
  • the other was reconstituted in 2 ml of MeOH and was used for flavonol analysis.
  • Each sample was stored at -34° C. until analyzed.
  • the flavonoids were quantified by injecting standards and comparing their peak areas with those from the plant samples. The results are expressed as ⁇ g of flavonoid/g fresh weight of plant material.
  • Kaempferol 3-rhamnoside could not be quantitated for several cultivars and is designated as ND (not determined).
  • the chromatograms showed a small, wide peak in the region of elution for this compound. If a substantial amount of this compound were present, a distinct peak appeared but minute quantities, if present, were masked.

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Abstract

This invention relates to a new distinct cultivar of geranium, substantially as illustrated and described, characterized as being particularly well adapted to both commercial greenhouse production and garden performance, and having more fully double florets, lighter flower color and more compact plant habit when compared to the geranium cultivar "Veronica".

Description

The present invention relates to a new and distinct cultivar of geranium Pelargonium×hortorum called "Cassandra". The cultivar is particularly well adapted to both commercial greenhouse production and garden performance. The novel characteristics of the cultivar are more fully double florets, lighter floral color, and more compact plant habit when compared to the cultivar geranium "Veronica". The cultivar is further characterized by unique biochemical fingerprint profiles.
The cultivar was developed from an organized, scientifically designed breeding program carried out at the Department of Horticulture, The Pennsylvania State University, University Park, PA 16802 and specifically resulted from selection from the self-pollinated progeny of the geranium cultivar "Honseler's Glorie Lila", which is probably an asexual selection from "Purpurball". The cultivar was asexually propagated by cuttings and the reproductions ran true.
DESCRIPTION OF THE FIGURES
FIG. 1 illustrates in color the cultivar including foliage and flowers.
FIG. 2 illustrates the anthocyanin profile obtained from HPLC. Quantitative values are found in the tables. Analyses included a single peak that represented both pelargonidin and petunidin 3,5-diglucosides. Corrections were made in accompanying tables.
Peak No.
1. Delphinidin 3,5-diglucoside
2. Cyanidin 3,5-diglucoside
3. Pelargonidin 3,5-diglucoside
4. Peonidin 3,5-diglucoside
5. Malvidin 3,5-diglucoside
FIG. 3 illustrates the flavonol profile obtained from HPLC. Quantitative values are found in the tables.
Peak No.
1. Quercetin 3-rhamnosylgalactoside
2. Quercetin 3-rutinoside
3. Quercetin 3-galactoside
4. Quercetin 3-glucoside
5. Kaempferol 3-rhamnosylgalactoside
6. Kaempferol 3-galactoside
7. Kaempferol 3-rutinoside
8. Kaempferol 3-glucoside; Kaempferol 7-glucoside; Quercetin 3-rhamnoside
9. Kaempferol 3-xyloside
10. Kaempferol 3-arabinoside
11. Kaempferol 3-rhamnoside
With reference to the detailed description of the cultivar which follows, the test plant was grown in full sun under glass, 60° F. night and 75° F. sunny days. Soilless medium was fertilized constantly with 300 ppm N-K. Color readings were taken under cool white fluorescent lamps at 220 foot-candles and color identification was by reference to The Royal Horticultural Society Colour Charts except where common terms of color definition are employed.
The Plant
Classification:
Botanical.--Pelargonium×hortorum.
Tradename.--#725-(80-208-1)="Cassandra".
Form: Semi-dwarf, free basal branching; comparatively compact growth; flowers relatively close to foliage; freer flowering; earlier flowering; significantly improved form in this color.
Height: 21.0-26.0 cm.
Growth: Faster than standard; leaves smaller than standard; short internodes; free branching from base; leaves zoned.
Strength: Stands upright without artificial support.
Leaves:
Size.--8.0-12.0 cm.
Shape.--Reniform, variously lobed.
Margin.--Crenate.
Texture.--Pubescent, dull; veins prominent and recessed.
Color.--Top: Dark zone band, Fan 3, green group 143-A (R.H.S.C.C.); Rest of leaf, Fan 3, yellow-green group, 144-A (R.H.S.C.C.). Bottom: Fan 3, green group, 143-C (R.H.S.C.C.).
Ribs and veins.--Palmate.
Petioles: Fan 3, green group, 143-C (R.H.S.C.C.).
Stem:
Color.--Fan 3, green group, 143-C (R.H.S.C.C.).
Internodes.--1.0-2.0 cm.
The Bud
Shape: Slight crook to petiole just below bud; hemispherical.
Size: 2.0-3.0 cm diameter.
Inflorescence
Blooming habit: Continuous, upright, double, non-shattering hemispherical.
Size: 10.0-12.0 cm.
Borne: Umbel; florets on pedicel; pedicels on peduncle.
Florets:
Form.--Petals twisted, upright, irregular and variable, double.
Color.--Top: Upper: Fan 2, red-purple group, 66-B (R.H.S.C.C.). Middle: Fan 2, red-purple group, 57-B (R.H.S.C.C.). Base: Fan 1, red group, 41-C (R.H.S.C.C.). Bottom: Fan 1, red group, 55-B (R.H.S.C.C.).
Petals.--11-14, including petaloids.
Size.--4.0 cm.
Texture and appearance.--Irregular-surfaced; dull.
Petaloids:
Quantity.--Not distinguishable from petals.
Shape.--Not distinguishable from petals.
Color.--Not distinguishable from petals.
Pedicel:
Length.--4.0-5.0 cm.
Color.--Fan 3, green group, 143-C (R.H.S.C.C.).
Peduncle: Arises from node; opposed to leaf petiole.
Length.--11.0-15.0 cm.
Color.--Fan 3, yellow-green group, 143-C (R.H.S.C.C.).
Persistence: Non-shattering, persistent
Disease resistance: Not known; favorable in outdoor trials
Lasting quality: Excellent, 3 weeks or longer
Reproductive Organs
Stamens: 4.
Anthers.--Some sterile, some fertile; tan to purple in color.
Filaments.--Flattened, ribbon shape; white at bottom, reddish tinge at top; variable in length, 4.0-8.0 mm.
Pollen.--Gold color.
Pistils:
Number.--1, with 5-parted stigma, reflexed.
Length.--1.0 cm.
Stigma.--5- or 6-parted, reddish in color.
Style.--1:3.0 mm length; light pink.
Ovaries: 1: green, very pubescent; 5- or 6- lobed, 5.0 mm in length.
Fruit: None observed.
Biochemical Profiles
In recent years, biochemical analysis has played an increasing role in plant systematics and taxonomy. In order to further characterize the cultivar, flavonols and anthocyanins were extracted from the florets and subjected to analysis by high pressure liquid chromatography (HPLC). Background information supporting the validity of the HPLC technique can be found in an article by Asen & Griesbach ("High Pressure Liquid Chromatographic Analysis of Flavonoids in Geranium Florets as an Adjunct for Cultivar Identification", S. Asen and R. Griesbach, J. Amer. Soc. Hort. Sci. 108(5):845-850 (1983)), the contents of which are incorporated herein by reference. Briefly, the method for performing the analysis was carried out as follows:
Flavonoid extraction. The sample size for flavonoid identification consisted of the petals from six florets just after anthesis. Three different samples were collected from each cultivar and handled separately for analysis. The petals were weighed, ground in 20 ml of 1% HCl-MeOH with a mortar and pestle, filtered through one layer of Whatman #1 filter paper, and washed with 1% HCl-MeOH. The volume was adjusted to 90 ml and 2-15 ml aliquots were removed for the analysis and handled separately. Each aliquot was taken to dryness at 40° C. in vacuo. All traces of HCl were removed by azeotropic distillation with MeOH. One of the dried extracts was reconstituted in 2 ml of 1% HCl-MeOH and was used for anthocyanin analysis. The other was reconstituted in 2 ml of MeOH and was used for flavonol analysis. Each sample was stored at -34° C. until analyzed.
HPLC. Samples were analyzed on a Waters High Performance Liquid Chromatograph equipped with an automatic injection system (Waters Assoc. Wisp 71OA), dual pumps (Waters Assoc. Model 6000A), solvent programmer (Waters Assoc. Model 600), data module (Waters Assoc.), variable wavelength detector (Waters Assoc. Model 480), and a C18 column (25 cm×0.46 cm and 5 μm particle size, Supelco).
Most of the flavonol compounds were separated by a linear gradient of 8% to 23% pump B over 55 min (pump A=1% triethylamine buffered to pH 3.0 with H3 PO4 (TEAP); pump B=CH3 CN) at a flow rate of 1.2 ml/min and a chart speed of 0.5 cm/min. Detection was at 340 nm.
The anthocyanins were resolved by a linear gradient of 30% to 50% pump B over 40 min (pump A=1.5% H3 PO4 ; pump B=20% HOAc+25% CH3 CN+55% of 1.5% H3 PO4) at a flow rate of 1.0 ml/min and a chart speed of 0.5 cm/min. Detection was at 546 nm utilizing a fixed wavelength detector.
The flavonoids were quantified by injecting standards and comparing their peak areas with those from the plant samples. The results are expressed as μg of flavonoid/g fresh weight of plant material.
Results
Chromatographic profiles for anthocyanins and flavonols are presented in FIGS. 2 and 3, respectively; quantification of these profiles by comparison to standards is presented in Tables 1 and 2, respectively.
The anthocyanins petunidin and pelargonidin 3,5-diglucoside were not resolved by the solvent system used. Past research has shown only negligible amounts of petunidin 3,5-diglucoside to be present in geranium florets compared to pelargonidin 3,5-diglucoside. In light of this, the peak corresponding to petunidin and pelargonidin 3,5-diglucoside was quantified as palargonidin 3,5-diglucoside.
Kaempferol 3-rhamnoside could not be quantitated for several cultivars and is designated as ND (not determined). The chromatograms showed a small, wide peak in the region of elution for this compound. If a substantial amount of this compound were present, a distinct peak appeared but minute quantities, if present, were masked.
Barriers to quantitation of several flavonols existed. Kaempferol 3-glucoside, kaempferol 7-glucoside, and quercetin 3-rhamnoside all had the same retention time under these conditions. If these compounds are needed to distinguish between cultivars, they would have to be separated by other solvents or column types. Quercetin 3-xyloside appeared in several of the comparisons, but standards were not available to quantify this compound.
              TABLE 1                                                     
______________________________________                                    
Anthocyanin concentration in petals of geranium florets                   
μg anthocyanidin 3,5-diglucoside/g fresh wt.                           
       Del-              Pelar-       Mal-                                
Cultivar                                                                  
       phinidin Cyanidin gonidin                                          
                               Peonidin                                   
                                      vidin                               
                                           Total                          
______________________________________                                    
725    19       32       576   509    489  1625                           
______________________________________                                    

              TABLE 2                                                     
______________________________________                                    
Flavonol concentration in petals of geranium florets                      
______________________________________                                    
μg/g fresh wt.                                                         
       Qu3-.sup.z                                                         
               Qu3-   Qu3- Qu3-  Km3-  Km3-  Km3-                         
Cultivar                                                                  
       rhagal  rut    gal  glu   rhagal                                   
                                       gal   rut                          
______________________________________                                    
725    t.sup.y 25     --.sup.w                                            
                           t     147   26    815                          
______________________________________                                    
               μg/g fresh wt.                                          
                 Km3-    Km3-    Km3-                                     
          Cultivar                                                        
                 xyl     arab    rha   Total                              
______________________________________                                    
          725    18      44      122   1207                               
______________________________________                                    
 .sup.z Abbreviations: Km = Kaempferol; Qu = Quercetin; arab = arabinoside
 gal = galactoside; glu = glucoside; rha = rhamnoside; rhagal =           
 rhamnosylgalactoside; rut = rutinoside; xyl = xyloside.                  
 .sup.y t = trace < 10 μg.                                             
 .sup.w -- = not detected.

Claims (1)

What is claimed is:
1. A new distinct cultivar of geranium, substantially as illustrated and described, characterized as being particularly well adapted to both commercial greenhouse production and garden performance, and having more fully double florets, lighter flower color and more compact plant habit when compared to the geranium cultivar "Veronica".
US06/882,119 1986-07-03 1986-07-03 Geranium plant "Cassandra" Expired - Lifetime USPP6220P (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6291746B1 (en) * 1988-09-05 2001-09-18 Syngenta Seeds B.V. Plant breeding

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6291746B1 (en) * 1988-09-05 2001-09-18 Syngenta Seeds B.V. Plant breeding

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Owner name: RESEARCH CORPORATION, 25 BROADWAY, NEW YORK, NEW Y

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNOR:CRAIG, RICHARD;REEL/FRAME:004575/0632

Effective date: 19860625