USPP6219P - Geranium plant "Paris" - Google Patents

Geranium plant "Paris" Download PDF

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Publication number
USPP6219P
USPP6219P US06/882,070 US88207086V US6219P US PP6219 P USPP6219 P US PP6219P US 88207086 V US88207086 V US 88207086V US 6219 P US6219 P US 6219P
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cultivar
geranium
color
kaempferol
florets
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US06/882,070
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Richard Craig
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Research Corp
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Research Corp
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  • the present invention relates to a new and distinct cultivar of geranium Pelargonium ⁇ hortorum called "Paris”.
  • the cultivar is particularly well adapted to both commercial greenhouse production as well as garden performance.
  • the cultivar's novel characteristics include more doubleness in the florets and a distinct flower color when compared to the parent "Honseler's Glorie Lila”.
  • the cultivar is further characterized by unique biochemical fingerprint profiles.
  • the cultivar was developed from an organized, scientifically designed breeding program carried out at the Department of Horticulture, The Pennsylvania State University, University Park, PA 16802 and specifically resulted from selection from the self-pollinated progeny of the geranium cultivar "Honseler's Glorie Lila” (which probably was an asexual selection from "Purpurball”). The cultivar was asexually propagated by cuttings and the reproduction ran true.
  • FIG. 1 illustrates in color the cultivar including foliage and flowers.
  • FIG. 2 illustrates the anthocyanin profile obtained from HPLC. Quantitative values are found in the tables. Analyses included a single peak that represented both pelargonidin and petunidin 3,5-diglucosides. Corrections were made in accompanying tables.
  • FIG. 3 illustrates the flavonol profile obtained from HPLC. Quantitative values were found in the tables.
  • test plant was grown in full sun under glass, 60° F. night and 75° F. sunny days. Soilless medium was fertilized constantly with 300 ppm N-K. Color readings were taken under cool white fluorescent lamps at 220 footcandles and color identification was by reference to The Royal Horticultural Society Colour Charts except where common terms of color definition are employed.
  • Form Semi-dwarf; free basal branching; comparatively compact growth; flowers relatively close to foliage; freer flowering; earlier flowering. Major improvement in form for this color range.
  • Petioles Fan 3, yellow-green group, 146-D (R.H.S.C.C.).
  • Shape Upright, hemispherical cluster.
  • Blooming habit Continuous, upright, full double, non-shattering, hemispherical in shape.
  • Fan 2 red-purple group, 67-B (R.H.S.C.C.).
  • Lens Fan 2, red-purple color group, 66-A(R.H.S.C.C.).
  • Bottom Veins whitish, Fan 4, white group, 155-B; interveins Fan 2, red-purple group, 65-A (R.H.S.C.C.).
  • Petal.--18-20 in number irregular, twisted and upright; full double; reflexed.
  • Peduncle Arises from node; opposed to leaf petiole.
  • Lasting quality Excellent, 3 weeks or longer.
  • Style.--1 style 1.0-2.0 mm long; similar to stigma in color.
  • Ovaries 1 ovary; 5.0-6.0 mm; 4- to 6-lobed; green, very pubescent.
  • Flavonoid extraction The sample size for flavonoid identification consisted of the petals from six florets just after anthesis. Three different samples were collected from each cultivar and handled separately for analysis. The petals were weighed, ground in 20 ml of 1% HCl-MeOH with a mortar and pestle, filtered through one layer of Whatman #1 filter paper, and washed with 1% HCl-MeOH. The volume was adjusted to 90 ml and 2-15 ml aliquots were removed for the analysis and handled separately. Each aliquot was taken to dryness at 40° C. in vacuo. All traces of HCl were removed by azeotropic distillation with MeOH.
  • One of the dried extracts was reconstituted in 2 ml of 1% HCl-MeOH and was used for anthocyanin analysis.
  • the other was reconstituted in 2 ml of MeOH and was used for flavonol analysis.
  • Each sample was stored at -34° C. until analyzed.
  • the flavonoids were quantified by injecting standards and comparing their peak areas with those from the plant samples. The results are expressed as ⁇ g of flavonoid/g fresh weight of plant material.
  • Kaempferol 3-rhamnoside could not be quantitated for several cultivars and is designated as ND (not determined).
  • the chromatograms showed a small, wide peak in the region of elution for this compound. If a substantial amount of this compound were present, a distinct peak appeared but minute quantities, if present, were masked.

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  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

This invention relates to a new distinct cultivar of geranium substantially as illustrated and described, characterized as being particularly well adapted to both commercial greenhouse production and garden performance, and possessing unique flavonol and anthocyanin profiles and more fully double flowers when compared to geranium cultivar "Honseler's Glorie Lila".

Description

The present invention relates to a new and distinct cultivar of geranium Pelargonium×hortorum called "Paris". The cultivar is particularly well adapted to both commercial greenhouse production as well as garden performance. The cultivar's novel characteristics include more doubleness in the florets and a distinct flower color when compared to the parent "Honseler's Glorie Lila". The cultivar is further characterized by unique biochemical fingerprint profiles.
The cultivar was developed from an organized, scientifically designed breeding program carried out at the Department of Horticulture, The Pennsylvania State University, University Park, PA 16802 and specifically resulted from selection from the self-pollinated progeny of the geranium cultivar "Honseler's Glorie Lila" (which probably was an asexual selection from "Purpurball"). The cultivar was asexually propagated by cuttings and the reproduction ran true.
DESCRIPTION OF THE FIGURES
FIG. 1 illustrates in color the cultivar including foliage and flowers.
FIG. 2 illustrates the anthocyanin profile obtained from HPLC. Quantitative values are found in the tables. Analyses included a single peak that represented both pelargonidin and petunidin 3,5-diglucosides. Corrections were made in accompanying tables.
Peak No.
1. Delphinidin 3,5-diglucoside
2. Cyanidin 3,5-diglucoside
3. Pelargonidin 3,5-diglucoside
4. Peonidin 3,5-diglucoside
5. Malvidin 3,5-diglucoside
FIG. 3 illustrates the flavonol profile obtained from HPLC. Quantitative values were found in the tables.
Peak No.
1. Quercetin 3-rhamnosylgalactoside
2. Quercetin 3-rutinoside
3. Quercetin 3-galactoside
4. Quercetin 3-glucoside
5. Kaempferol 3-rhamnosylgalactoside
6. Kaempferol 3-galactoside
7. Kaempferol 3-rutinoside
8. Kaempferol 3-glucoside; Kaempferol 7-glucoside; Quercetin 3-rhamnoside
9. Kaempferol 3-xyloside
10. Kaempferol 3-arabinoside
11. Kaempferol 3-rhamnoside
With reference to the detailed description of the cultivar which follows, the test plant was grown in full sun under glass, 60° F. night and 75° F. sunny days. Soilless medium was fertilized constantly with 300 ppm N-K. Color readings were taken under cool white fluorescent lamps at 220 footcandles and color identification was by reference to The Royal Horticultural Society Colour Charts except where common terms of color definition are employed.
The Plant
Clssification:
Botanical.--Pelargonium×hortorum.
Trade name.--728 (80-208-2)="Paris".
Form: Semi-dwarf; free basal branching; comparatively compact growth; flowers relatively close to foliage; freer flowering; earlier flowering. Major improvement in form for this color range.
Height: 23.0-30.0 cm.
Growth: Faster than standard, leaves smaller than standard; short internodes; free branching from base; no leaf zones.
Strength: Stands upright, without artificial support.
Leaves:
Size.--9.0-15.0 cm.
Shape.--Reniform, variously lobed.
Margin.--Crenate.
Texture.--Pubescent, dull (non-reflective).
Color.--Top: Fan 3, green group, 137-B (R.H.S.C.C.). Bottom: Fan 3, yellow-green group, 146-B (R.H.S.C.C.).
Ribs and veins: Palmate.
Petioles: Fan 3, yellow-green group, 146-D (R.H.S.C.C.).
Stem:
Color.--Same as petioles.
Internodes.--1.5-3.0 cm.
The Bud
Shape: Upright, hemispherical cluster.
Size: 2.0 cm across.
Inflorescence
Blooming habit: Continuous, upright, full double, non-shattering, hemispherical in shape.
Size: 10.0-12.0 cm across.
Borne: Umbel; florets on pedicel; pedicels on peduncle.
Florets:
Form.--Petals irregular, twisted and upright; full double; sepals irregular in number 5-8.
Color.--Top: Fan 2, red-purple group, 67-B (R.H.S.C.C.). Lens: Fan 2, red-purple color group, 66-A(R.H.S.C.C.). Bottom: Veins whitish, Fan 4, white group, 155-B; interveins Fan 2, red-purple group, 65-A (R.H.S.C.C.).
Petal.--18-20 in number (including petaloids) irregular, twisted and upright; full double; reflexed.
Size.--3.0-4.0 cm across.
Texture and appearance.--Irregular surface, reflexed, dull.
Petaloids:
Quantity.--Cannot distinguish from petals.
Shape.--Cannot distinguish from petals.
Color.--Cannot disitnguish from petals.
Pedicel:
Length.--3.0-4.0 cm.
Color.--Fan 3, yellow-green group, 146-D (R.H.S.C.C.).
Peduncle: Arises from node; opposed to leaf petiole.
Length.--16.0-20.0 cm.
Color.--Fan 3, yellow-green group, 146-C (R.H.S.C.C).
Persistence: Persistent, non-shattering.
Disease resistance: Not known.
Lasting quality: Excellent, 3 weeks or longer.
Reproductive Organs
Stamens: 3-4.
Anthers.--Highly sterile; most anthers undeveloped, usually one fertile anther per floret; tan in color.
Filaments.--Twisted, ribbon-like, resembling petaloids about 0.5 cm in length; color nondescript.
Pistils:
Number.--1 pistil, with 4-6 stigmatic lobes.
Length.--1.0 cm.
Stigma.--4- to 6-lobed; purplish, similar to upper petal color.
Style.--1 style; 1.0-2.0 mm long; similar to stigma in color.
Ovaries: 1 ovary; 5.0-6.0 mm; 4- to 6-lobed; green, very pubescent.
Fruit: None observed.
Biochemical Profiles
In recent years, biochemical analysis has played an increasing role in plant systematics and taxonomy. In order to further characterize the cultivar, flavonols and anthocyanins were extracted from the florets and subjected to analysis by high pressure liquid chomatography (HPLC). Background information supporting the validity of the HPLC technique can be found in an article by Asen & Griesbach ("High Pressure Liquid Chromatographic Analysis of Flavonoids in Geranium Florets as an Adjunct for Cultivar Identification", S. Asen and R. Griesbach, J. Amer. Soc. Hort. Sci. 108(5):845-850 (1983)), the contents of which are incorporated herein by reference. Briefly, the method for performing the analysis was carried out as follows:
Flavonoid extraction. The sample size for flavonoid identification consisted of the petals from six florets just after anthesis. Three different samples were collected from each cultivar and handled separately for analysis. The petals were weighed, ground in 20 ml of 1% HCl-MeOH with a mortar and pestle, filtered through one layer of Whatman #1 filter paper, and washed with 1% HCl-MeOH. The volume was adjusted to 90 ml and 2-15 ml aliquots were removed for the analysis and handled separately. Each aliquot was taken to dryness at 40° C. in vacuo. All traces of HCl were removed by azeotropic distillation with MeOH. One of the dried extracts was reconstituted in 2 ml of 1% HCl-MeOH and was used for anthocyanin analysis. The other was reconstituted in 2 ml of MeOH and was used for flavonol analysis. Each sample was stored at -34° C. until analyzed.
HPLC. Samples were analyzed on a Waters High Performance Liquid Chromatograph equipped with an automatic injection system (Waters Assoc. Wisp 710A), dual pumps (Waters Assoc. Model 6000A), solvent programmer (Waters Assoc. Model 600), data module (Waters Assoc.), variable wavelength detector (Waters Assoc. Model 480), and a C18 column (25 cm×0.46 cm and 5 μm particle size, Supelco).
Most of the flavonol compounds were separated by a linear gradient of 8% to 23% pump B over 55 min (pump A=1% triethylamine buffered to pH 3.0 with H3 PO4 (TEAP); pump B=CH3 CN) at a flow rate of 1.2 ml/min and a chart speed of 0.5 cm/min. Detection was at 340 nm.
The anthocyanins were resolved by a linear gradient of 30% to 50% pump B over 40 min (pump A=1.5% H3 PO4 ; pump B=20% HOAc+25% CH3 CN+55% of 1.5% H3 PO4) at a flow rate of 1.0 ml/min and a chart speed of 0.5 cm/min. Detection was at 546 nm utilizing a fixed wavelength detector.
The flavonoids were quantified by injecting standards and comparing their peak areas with those from the plant samples. The results are expressed as μg of flavonoid/g fresh weight of plant material.
Results
Chromatographic profiles for anthocyanins and flavonols are presented in FIGS. 2 and 3, respectively; quantification of these profiles by comparison to standards is presented in Tables 1 and 2, respectively.
The anthocyanins petunidin and pelargonidin 3,5-diglucoside were not resolved by the solvent system used. Past research has shown only negligible amounts of petunidin 3,5-diglucside to be present in geranium florets compared to pelargonidin 3,5-diglucoside. In light of this, the peak corresponding to petunidin and pelargonidin 3,5-diglucoside was quantified as pelargonidin 3,5-diglucoside.
Kaempferol 3-rhamnoside could not be quantitated for several cultivars and is designated as ND (not determined). The chromatograms showed a small, wide peak in the region of elution for this compound. If a substantial amount of this compound were present, a distinct peak appeared but minute quantities, if present, were masked.
Other barriers to quantitation of several flavonols existed. Kaempferol 3-glucoside, kaempferol 7-glucoside, and quercetin 3-rhamnoside all had the same retention time under these conditions. If these compounds are needed to distinguish between cultivars, they would have to be separated by other solvents or column types. Quercetin 3-xyloside appeared in several of the comparisons, but standards were not available to quantify this compound.
              TABLE 1                                                     
______________________________________                                    
Anthocyanin concentration in petals of geranium florets                   
μg anthocyanidin 3,5-diglucoside/g fresh wt.                           
       Del-              Pelar-       Mal-                                
Cultivar                                                                  
       phinidin Cyanidin gonidin                                          
                               Peonidin                                   
                                      vidin                               
                                           Total                          
______________________________________                                    
728    14       23       103   398    591  1129                           
______________________________________                                    

              TABLE 2                                                     
______________________________________                                    
Flavonol concentration in petals of geranium florets                      
______________________________________                                    
μg/g fresh wt.                                                         
       Qu3-.sup.z                                                         
               Qu3-   Qu3- Qu3-  Km3-  Km3-  Km3-                         
Cultivar                                                                  
       rhagal  rut    gal  glu   rhagal                                   
                                       gal   rut                          
______________________________________                                    
728    21      43     t.sup.y                                             
                           12    194   29    745                          
______________________________________                                    
               μg/g fresh wt.                                          
                 Km3-    Km3-    Km3-                                     
          Cultivar                                                        
                 xyl     arab    rha   Total                              
______________________________________                                    
          728    13      26      54    1142                               
______________________________________                                    
 .sup.z Abbreviations: Km = Kaempferol; Qu = Quercetin; arab = arabinoside
 gal = galactoside; glu = glucoside; rha = rhamnoside; rhagal =           
 rhamnosylgalactoside; rut = rutinoside; xyl = xyloside.                  
 .sup.y t = trace < 10 μg.

Claims (1)

What is claimed is:
1. A new distinct cultivar of geranium, substantially as illustrated and described, characterized as being particularly well adapted to both commercial greenhouse production and garden performance, and possessing unique flavonol and anthocyanin profiles, more fully double florets and distinct flower color when compared to geranium cultivar "Honseler's Glorie Lila".
US06/882,070 1986-07-03 1986-07-03 Geranium plant "Paris" Expired - Lifetime USPP6219P (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110041198A1 (en) * 2007-11-15 2011-02-17 Syngenta Participations Ag Flower pigmentation in pelargonium hortorum

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110041198A1 (en) * 2007-11-15 2011-02-17 Syngenta Participations Ag Flower pigmentation in pelargonium hortorum
US11134634B2 (en) 2007-11-15 2021-10-05 Syngenta Participations Ag Flower pigmentation in pelargonium hortorum

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Owner name: RESEARCH CORPORATION, 25 BROADWAY, NEW YORK, NEW Y

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNOR:CRAIG, RICHARD;REEL/FRAME:004586/0001

Effective date: 19860625

Owner name: RESEARCH CORPORATION,NEW YORK

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