USPP32842P3 - Shiitake mushroom named ‘HOKSY 10gokin’ - Google Patents

Shiitake mushroom named ‘HOKSY 10gokin’ Download PDF

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USPP32842P3
USPP32842P3 US15/932,645 US201815932645V USPP32842P3 US PP32842 P3 USPP32842 P3 US PP32842P3 US 201815932645 V US201815932645 V US 201815932645V US PP32842 P3 USPP32842 P3 US PP32842P3
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hoksy
10gokin
mushroom
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hyphae
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Genki SAKUTA
Momoko Aizawa
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Hokuto Corp
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H15/00—Fungi; Lichens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor

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  • This invention relates to a new and distinct variety of Shiitake mushroom, Lentinula edodes (Berk.) Pegler.
  • This new variety named ‘HOKSY 10gokin’, cultivated by repeated breeding of Shiitake mushrooms having dominant traits, has a white stem of high quality and enhanced cultivation stability, and ensures presentable stability, reproducibility, and uniformity.
  • Shiitake mushroom is an edible mushroom having the highest production amount in Japan, which accounts for 69.1 billion yen in fresh shiitake mushrooms and 9.2 billion yen in dried shiitake mushrooms according to statistics in 2014.
  • the supply of shiitake mushrooms is influenced by weather and the like, therefore it is difficult to stably supply shiitake mushrooms.
  • our company has developed a ‘HOKSY 8gokin’ mushroom with a resistance to lamella breakage, and which has few malformations thus far. Nevertheless, the ‘HOKSY 8gokin’ mushroom had problems in terms of quality, such as coloring of the stem.
  • a ‘HOKSY 10gokin’ mushroom was developed with a white stem of high quality, as compared to the ‘HOKSY 8gokin’ mushroom, and had higher cultivation stability than the ‘HOKSY 8gokin’ mushroom.
  • the present invention is a new and distinct variety of mushroom characterized particularly by its good qualitative character and appearance, a white stem of high quality, and enhanced cultivation, which can be cultivated by gathering and repeated breeding of fungal strains having dominant traits and isaki in stability, reproducibility and uniformity when being produced.
  • This novel and distinct variety of mushroom is identified as ‘HOKSY 10gokin’.
  • FIG. 1 shows a phylogenetic tree illustrating the antecedents of ‘HOKSY 10gokin’, ‘HOKSY 3gokin’ and ‘JMS 5K-16’ strains.
  • FIGS. 2A and 2B respectively show front and back images of a dual-culture of ‘HOKSY 10gokin’ colony.
  • FIGS. 3A and 3B respectively show front and back images of a dual-culture of ‘HOKSY 10gokin’ and ‘JMS 5K-16’ strains.
  • FIGS. 4A and 4B respectively show front and back images of a dual-culture of ‘HOKSY 10gokin’ and ‘HOKSY 3gokin’ strains.
  • FIG. 5 shows an image of the surface of fungal flora of ‘HOKSY 10gokin’.
  • FIG. 6 shows an image of the surface of fungal flora of ‘JMS 5K-16’.
  • FIG. 7 shows an image of the surface of fungal flora of ‘HOKSY 3gokin’.
  • FIG. 8 shows an image of fruit bodies of ‘HOKSY 10gokin’.
  • FIG. 9 shows an image of a fruit body of ‘JMS 5K-16’.
  • FIG. 10 shows an image of a fruit body of ‘HOKSY 3gokin’.
  • FIGS. 11A, 11B, and 11C respectively show top, underside, and cross-sectional images of a fruit body of ‘HOKSY 10gokin’.
  • FIGS. 12A, 12B, and 12C respectively show top, underside, and cross-sectional images of a fruit body of ‘JMS 5K-16’.
  • FIGS. 13A, 13B, and 13C respectively show top, underside, and cross-sectional images of a fruit body of ‘HOKSY 3gokin’.
  • ‘HOKSY 10gokin’ mushroom was discovered in Nagano-shi, Nagano, Japan where the temperature, humidity and carbon dioxide concentration are controlled. ‘HOKSY 10gokin’ was asexually reproduced by inoculating on a potato dextrose agar with hyphae and incubating in a culture room set at 25° C. The history of the ‘HOKSY 10gokin’ mushroom in terms of improvement period and the like are set forth in the following chronological list of each stage of variety improvement:
  • the amount of aerial hyphae development when growing hyphae on an agar medium is smaller than that of the parent varieties ‘HOKSY 8gokin’, and the Density of hyphae is lower than that of the parent varieties ‘HOKSY 3gokin’ and ‘HOKSY 8gokin’.
  • the shape of the gill is different from that of ‘HOKSY 3gokin’.
  • the stipe length is shorter than that of ‘HOKSY 8gokin’.
  • the ‘HOKSY 10gokin’ mushroom has the following characteristics: a white stem of high quality, and enhanced cultivation.
  • Dual culture was performed for the ‘HOKSY 10gokin’ mushroom and a similar variety so as to examine whether or not a zone line is formed.
  • a potato dextrose agar medium was used, and the ‘HOKSY 10gokin’ mushroom and the similar variety were inoculated thereon face to face at an interval of 3 cm, and then culture was performed at 25° C. for 28 days to examine whether or not a zone line was formed.
  • JMS 5K-16 Variety similar to the present variety
  • preculture was performed at 25° C. for 4 days so as to make the regeneration of hyphae equal (about 10 mm in diameter), and then culture was performed for 7 days at intervals of 5° C. between 5° C. and 30° C.
  • An average daily hyphae growth rate was calculated based on a hyphae growth rate for seven days of the culture.
  • Mushroom bed bag A mushroom bed bag made of polyethylene (117 mm ⁇ 230 mm, 35 ⁇ ) was used.
  • Culture medium Sawdust, rice bran and wheat bran were mixed at the dry weight ratio of 8:1:1, and the water content was adjusted to 63%. The amount of a medium filled was 1.5 kg per bag, and high-pressure sterilization was performed.
  • Starter culture About 20 mL of sawdust starter cultures per bottle was inoculated.
  • Culture Culture was performed at 20° C. for 85 days at 60-70% moisture.
  • Table 2 shows the characteristics of the ‘HOKSY 10gokin’ and specific difference in characteristics as compared with the similar variety when culture was performed under the abovementioned conditions.

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Abstract

The present variety of mushroom named ‘HOKSY 10gokin’ was cultivated by the gathering and repeated breeding of Shiitake mushrooms having dominant traits, which has good qualitative character and appearance, a white stem of high quality, and enhanced cultivation. This edible mushroom is exquisite in stability, reproducibility and uniformity when being produced.

Description

BACKGROUND OF THE INVENTION
This invention relates to a new and distinct variety of Shiitake mushroom, Lentinula edodes (Berk.) Pegler. This new variety named ‘HOKSY 10gokin’, cultivated by repeated breeding of Shiitake mushrooms having dominant traits, has a white stem of high quality and enhanced cultivation stability, and ensures presentable stability, reproducibility, and uniformity.
Shiitake mushroom is an edible mushroom having the highest production amount in Japan, which accounts for 69.1 billion yen in fresh shiitake mushrooms and 9.2 billion yen in dried shiitake mushrooms according to statistics in 2014. However, since those who engage in the production of shiitake mushrooms are mainly subsistence farmers, the supply of shiitake mushrooms is influenced by weather and the like, therefore it is difficult to stably supply shiitake mushrooms. Accordingly, there is a strong demand in the market for a stable supply of shiitake mushrooms by our company, and therefore our company has been conducting a wide variety of studies for the stable production of shiitake mushrooms. Our company has developed a ‘HOKSY 8gokin’ mushroom with a resistance to lamella breakage, and which has few malformations thus far. Nevertheless, the ‘HOKSY 8gokin’ mushroom had problems in terms of quality, such as coloring of the stem.
As a result of continuing breed improvement by cross breeding so as to improve the above problem and further enhance cultivation stability, a ‘HOKSY 10gokin’ mushroom was developed with a white stem of high quality, as compared to the ‘HOKSY 8gokin’ mushroom, and had higher cultivation stability than the ‘HOKSY 8gokin’ mushroom.
SUMMARY OF THE INVENTION
The present invention is a new and distinct variety of mushroom characterized particularly by its good qualitative character and appearance, a white stem of high quality, and enhanced cultivation, which can be cultivated by gathering and repeated breeding of fungal strains having dominant traits and is exquisite in stability, reproducibility and uniformity when being produced. This novel and distinct variety of mushroom is identified as ‘HOKSY 10gokin’.
BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 shows a phylogenetic tree illustrating the antecedents of ‘HOKSY 10gokin’, ‘HOKSY 3gokin’ and ‘JMS 5K-16’ strains.
FIGS. 2A and 2B respectively show front and back images of a dual-culture of ‘HOKSY 10gokin’ colony.
FIGS. 3A and 3B respectively show front and back images of a dual-culture of ‘HOKSY 10gokin’ and ‘JMS 5K-16’ strains.
FIGS. 4A and 4B respectively show front and back images of a dual-culture of ‘HOKSY 10gokin’ and ‘HOKSY 3gokin’ strains.
FIG. 5 shows an image of the surface of fungal flora of ‘HOKSY 10gokin’.
FIG. 6 shows an image of the surface of fungal flora of ‘JMS 5K-16’.
FIG. 7 shows an image of the surface of fungal flora of ‘HOKSY 3gokin’.
FIG. 8 shows an image of fruit bodies of ‘HOKSY 10gokin’.
FIG. 9 shows an image of a fruit body of ‘JMS 5K-16’.
FIG. 10 shows an image of a fruit body of ‘HOKSY 3gokin’.
FIGS. 11A, 11B, and 11C respectively show top, underside, and cross-sectional images of a fruit body of ‘HOKSY 10gokin’.
FIGS. 12A, 12B, and 12C respectively show top, underside, and cross-sectional images of a fruit body of ‘JMS 5K-16’.
FIGS. 13A, 13B, and 13C respectively show top, underside, and cross-sectional images of a fruit body of ‘HOKSY 3gokin’.
 DETAILED DESCRIPTION OF THE INVENTION
‘HOKSY 10gokin’ mushroom was discovered in Nagano-shi, Nagano, Japan where the temperature, humidity and carbon dioxide concentration are controlled. ‘HOKSY 10gokin’ was asexually reproduced by inoculating on a potato dextrose agar with hyphae and incubating in a culture room set at 25° C. The history of the ‘HOKSY 10gokin’ mushroom in terms of improvement period and the like are set forth in the following chronological list of each stage of variety improvement:
March 2009: Cultivation of ‘MH009092’ strain.
March 2014: Cultivation of ‘MH009106’ strain.
June 2017: ‘MH009092’ and ‘MH009106’ strains were crossed and an excellent strain ‘MH009107’ was selected.
January 2018: Growing test was repeatedly conducted on ‘MH009107’ and since distinguishability, stability and uniformity were confirmed, the strain was named ‘HOKSY 10gokin’ and cultivation was completed. Applied for registration of a new variety to the Ministry of Agriculture, Forestry and Fisheries of Japan. Registered the Application No. 32805 on Jan. 24, 2018.
In physiological characteristics, the amount of aerial hyphae development when growing hyphae on an agar medium is smaller than that of the parent varieties ‘HOKSY 8gokin’, and the Density of hyphae is lower than that of the parent varieties ‘HOKSY 3gokin’ and ‘HOKSY 8gokin’. In morphological characteristics, the shape of the gill is different from that of ‘HOKSY 3gokin’. Also, the stipe length is shorter than that of ‘HOKSY 8gokin’.
The above crossing is summarized in the phylogenetic tree illustrated in FIG. 1.
The ‘HOKSY 10gokin’ mushroom has the following characteristics: a white stem of high quality, and enhanced cultivation.
(1) Comparison with Existing Variety by Dual Culture
Dual culture was performed for the ‘HOKSY 10gokin’ mushroom and a similar variety so as to examine whether or not a zone line is formed.
Study Method:
As an examination method, a potato dextrose agar medium was used, and the ‘HOKSY 10gokin’ mushroom and the similar variety were inoculated thereon face to face at an interval of 3 cm, and then culture was performed at 25° C. for 28 days to examine whether or not a zone line was formed.
Strain Used:
‘HOKSY 10gokin’: Present variety
‘JMS 5K-16’: Variety similar to the present variety
‘HOKSY 3gokin’: Variety similar to the present variety
Results:
No zone line was formed in the dual culture of ‘HOKSY 10gokin’ strains (Table 1), while a zone line was formed between the ‘HOKSY 10gokin’, and ‘JMS 5K-16’ and ‘HOKSY 3gokin’ strains (Table 1). This clearly shows that the present mushroom is a new variety.
TABLE 1
Results of dual culture
Similar variety Present variety
Similar variety ‘HOKSY ‘HOKSY
‘JMS 5K-16’ 3gokin’ 10gokin’
‘HOKSY 10 gokin’ + + −
+ is present and − is absent.
(2) Growth Characteristics of ‘HOKSY 10gokin’ (2)-1 Temperature Adaptation of Hyphae
Study Method:
After inoculating an agar piece of the ‘HOKSY 10gokin’ having a diameter of 5 mm and an agar piece of the similar variety having a diameter of 5 mm on a potato dextrose agar medium, preculture was performed at 25° C. for 4 days so as to make the regeneration of hyphae equal (about 10 mm in diameter), and then culture was performed for 7 days at intervals of 5° C. between 5° C. and 30° C. An average daily hyphae growth rate was calculated based on a hyphae growth rate for seven days of the culture.
Results:
There was no significant difference in hyphae elongation rate between the ‘HOKSY 10gokin’ mushroom and that of the similar varieties at each temperature zone (Table 2).
(2)-2 Comparison in the Formation of Hyphae Tunic, Aerial Hyphae, Hyphae Density and Tinting of the Surface of Fungal Flora
Study Method:
After inoculating an agar piece of the ‘HOKSY 10gokin’ having a diameter of 5 mm and an agar piece of the similar variety having a diameter of 5 mm on a potato dextrose agar medium, culture was performed at 25° C. for 14 days. For these two strains, comparative observation was performed with regard to hyphae tunic, aerial hyphae and tinting of the surface of fungal flora.
Results:
The formation of hyphae tunic was confirmed in the ‘HOKSY 10gokin’ mushroom, while it was not confirmed in the similar variety ‘JMS 5K-16’ (Table 2). With regard to aerial hyphae, there was no difference between the present variety and the similar varieties (Table 2). Regarding hyphae density, while for the ‘HOKSY 10gokin’ mushroom it is medium, it was dense in similar variety ‘HOKSY 3gokin’ (Table 2). Tinting of the surface of fungal flora was observed in the ‘HOKSY 10gokin’ mushroom, while it was not confirmed in the similar variety ‘JMS 5K-16’ (Table 2).
(3) Morphological Characteristics of the ‘HOKSY 10gokin’ Mushroom in a Cultivation Example
Cultivation Method:
Mushroom bed bag: A mushroom bed bag made of polyethylene (117 mm×230 mm, 35φ) was used.
Culture medium: Sawdust, rice bran and wheat bran were mixed at the dry weight ratio of 8:1:1, and the water content was adjusted to 63%. The amount of a medium filled was 1.5 kg per bag, and high-pressure sterilization was performed.
Starter culture: About 20 mL of sawdust starter cultures per bottle was inoculated.
Culture: Culture was performed at 20° C. for 85 days at 60-70% moisture.
Growth: After completing the culture, the mushroom bed was taken out of the bag and washed with water, and then mushrooms were grown at 13-14° C., at 90% moisture or higher at a CO2 concentration of 1,000 ppm or lower. An immersion method was used as the generation method on the second time and thereafter.
Cultivation Results:
Table 2 shows the characteristics of the ‘HOKSY 10gokin’ and specific difference in characteristics as compared with the similar variety when culture was performed under the abovementioned conditions.
Also, the whole, top, underside, and cross-sectional images of the respective fruit bodies have also been attached. (Refer to FIGS. 8 to 13).
TABLE 2
Fungus characteristics Table of Lentinula edodes (Berk.)
Pegler of Recording and Registration
Present Similar Similar
variety variety variety
‘HOKSY ‘JMS ‘HOKSY
10gokin’ 5K-16’ 3gokin’
Genetic property
Formation of zone line + + +
Physiological property
Formation of hyphae tunic + − +
Aerial hyphae medium medium medium
development
Density of hyphae medium medium dense
Tinting of surface of + − +
fungal flora
Temperature adaptability
Tolerance for High or
Low Temperature
Optimal temperature for 25 25 25
hyphal growth (° C.)
Hyphal growth rate at
each temperature
(mm/day)
5° C. 0.36 0.47 0.45
10° C. 1.47 1.42 1.40
15° C. 2.36 2.59 2.63
20° C. 4.31 4.42 4.48
25° C. 5.13 5.19 5.34
30° C. 2.96 3.45 3.10
Morphological property
Cap
Shape of top view round round round
Shape of vertical flat flat flat
cross section
Fleshy type quarter circle quarter circle quarter circle
shape shape shape shape
Diameter (mm) 46.31 38.83 40.01
Main color of apex brown brown brown
[RHS:200C] [RHS:200C] [RHS:200C]
Thickness (mm) 11.80 11.70 12.56
Hardness medium medium medium
Distribution of scales periphery periphery periphery
Size of scales medium medium medium
Coloring of scales + + +
[RHS: 158B] [RHS: 159B] [RHS: 158B]
Gill
Shape wedge shape rectangular rectangular
triangle triangle
shape shape
Arrangement ripple or straight ripple or
crinkle crinkle
Width (mm) 2.45 1.04 2.08
Density medium medium medium
Color cream cream cream
[RHS: 158B] [RHS: 159B] [RHS: 158B]
Stipe
Shape head portion head portion head portion
Length (mm) 30.05 41.86 34.17
Ratio of cap diameter/ 1.55 0.95 1.19
stipe length
Thickness (mm) 10.12 13.60 10.18
Ratio of cap diameter/ 4.63 2.92 3.99
stipe thickness
Tinting of surface + + +
[RHS: 159B] [RHS: 159B] [RHS: 159B]
Presence of fluff + + +
Tinting of fluff + + +
[RHS: 159B] [RHS: 159B] [RHS: 159B]
Hardness hard hard hard
Cultural property
Period from inoculation 70 70 70
to fruit induction (day)
Period from fruit in- 10.0 10.0 10.0
duction to harvest (day)
Type of fruiting concentrated concentrated concentrated
Soaking treatment suitable suitable suitable
Temperature of soaking 14 14 14
yield (° C.)
Method of secondary use jointly use jointly use jointly
fruiting
Fruiting temperature 14 14 14
(° C.)
Adaptability of culture broad leaved broad leaved broad leaved
medium
Ratio of dry weight 10.4 10.3 10.4
fruit body (%)
Average of 1 dry weight 1.9 2.0 1.9
fruit body (g/piece)
Yield
Yield of sawdust medium 2.72 3.08 3.09
100 kg (kg)
Ratio of every month
yield (%)
1st month 60.9 55.5 57.1
2nd month 14.4 10.5 13.8
3rd month 15.1 23.8 14.6
4th month 9.6 12.5 12.2
* The employed color chart is R.H.S Colour Chart, 2007, Fifth edition, prescribed by Royal Horticultural Society, England.

Claims (1)

What is claimed is:
1. A new, distinct variety of Shiitake mushroom as substantially illustrated and described in the specification.
US15/932,645 2018-01-24 2018-03-30 Shiitake mushroom named ‘HOKSY 10gokin’ Active 2039-02-07 USPP32842P3 (en)

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JP32805 1986-02-19
JPPBR32805 2018-01-24
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Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
PLUTO UPOVROM Plant Variety Database Citation for ‘HOKSY 10gokin’ as per JP PBR 32805; Jan. 25, 2018; 1 page. *
PLUTO UPOVROM Plant Variety Database Citation for 'HOKSY 10gokin' as per JP PBR 32805; Jan. 25, 2018; 1 page. *

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