USPP32830P3 - Camellia sinensis (L.) Kuntze plant named ‘Kiyoka’ - Google Patents

Camellia sinensis (L.) Kuntze plant named ‘Kiyoka’ Download PDF

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USPP32830P3
USPP32830P3 US16/602,869 US201916602869V USPP32830P3 US PP32830 P3 USPP32830 P3 US PP32830P3 US 201916602869 V US201916602869 V US 201916602869V US PP32830 P3 USPP32830 P3 US PP32830P3
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kiyoka
medium
yabukita
tea
saemidori
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US20200196503P1 (en
Inventor
Katsuyuki Yoshida
Akiko Ogino
Atsushi Nesumi
Tetsuji Saba
Junichi Tanaka
Fumiya Taniguchi
Shuya Yamashita
Hiroshi Yorozuya
Akiko Matsunaga
Yoshiyuki Takeda
Tsuyoshi Okamoto
Hitoshi Yoshitomi
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National Agriculture and Food Research Organization
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Assigned to NATIONAL AGRICULTURE AND FOOD RESEARCH ORGANIZATION reassignment NATIONAL AGRICULTURE AND FOOD RESEARCH ORGANIZATION ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: TANAKA, JUNICHI, OKAMOTO, TSUYOSHI, TAKEDA, YOSHIYUKI, Yoshitomi, Hitoshi, MATSUNAGA, AKIKO, Nesumi, Atsushi, OGINO, AKIKO, Saba, Tetsuji, Taniguchi, Fumiya, Yamashita, Shuya, Yorozuya, Hiroshi, YOSHIDA, KATSUYUKI
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12—Leaves

Definitions

  • Camellia sinensis (L.) Kuntze The claimed plant is a hybridization of different varieties.
  • Camellia sinensis (L.) Kuntze commonly called tea plant or tea shrub, is an evergreen shrub or small tree whose leaves and leaf buds are used to produce tea.
  • Camellia sinensis is mainly cultivated in tropical and subtropical climates.
  • Camellia sinensis is native to East Asia, the Indian Subcontinent, and Southeast Asia, however it is currently cultivated across the world in tropical and subtropical regions.
  • ‘Kiyoka’ is a new variety of Camellia sinensis (L.) Kuntze bred by crossbreeding FYZ-41 (‘Yabukita’ x ‘Shizuinzatsu 131’), a hybrid, as the female parent and ‘Saemidori’ as the male parent.
  • ‘Kiyoka’ is propagated by grafts or cuttings. The plant may be used, e.g., for cultivation of tea leaves and leaf buds for consumption.
  • ‘Kiyoka’ has been asexually reproduced in Kagoshima prefecture, Japan.
  • ‘Kiyoka’ is propagated by cutting. Cutting is a method in which a young shoot is adjusted to a cutting having one leaf or two leaves, and the cutting is then inserted into soil, or the like, so as to take root.
  • ‘Kiyoka’ is a variety obtained by selecting individuals from progeny of a crossbreed between (i) FYZ-41 (seed parent), which is an Assam variant crossbreed progeny having a floral fragrance, and (ii) ‘Saemidori’, which is a high-quality variety having strong umami. ‘Kiyoka’ is characterized in that (i) it has a clean and distinctive floral fragrance like that of an oriental orchid, (ii) its color of liquor is greenish, and (iii) it has a flavor exhibiting umami. ‘Kiyoka’ is early budding and thus limited in terms of cultivation region, and its yield is less than that of ‘Saemidori’. However, ‘Kiyoka’ presents the possibility of development of new products which utilize its new characteristic aroma and flavor.
  • FIG. 1 is a breeding pedigree chart for ‘Kiyoka’.
  • FIG. 2 is a photograph of ‘Kiyoka’ first flush, young shoots.
  • FIG. 3 is a photograph of the state of ‘Kiyoka’ plants at time of first flush.
  • FIG. 4 is a photograph of the state of ‘Kiyoka’ plants in the cultivation field at the time of plucking. Ruler shown is 1 meter.
  • FIG. 5 is a photograph of the state of parent variety ‘Saemidori’ plants in the cultivation field at the time of plucking. Ruler shown is 1 meter.
  • FIG. 6 is a photograph of the state of grandparent variety ‘Yabukita’ plants in the cultivation field at the time of plucking. Ruler shown is 1 meter.
  • FIG. 7 is a photograph of the shape of young shoots at plucking (first flush) of ‘Kiyoka’ (left), ‘Saemidori’ (center), and ‘Yabukita’ (right).
  • FIG. 8 is a photograph of the shape of young leaves from water sprout at plucking (first flush) for ‘Kiyoka’ (left), ‘Yabukita’ (center), and ‘Saemidori’ (right).
  • Common names of the claimed plant's species include: tea plant or tea shrub.
  • ‘FYZ-41’ was used as a female parent because it had the target characteristic scent of flowers, however specific characteristic data concerning ‘FYZ-41’ is not available.
  • ‘Kiyoka’ was selected from this F1 seedling group in an individual selection test in 2005 (see FIG. 1 ). Individual selection tests were performed from 2001 to 2005. Thereafter, ‘Kiyoka’ was submitted under the name ‘Makurazaki 54-20’ to clonal line comparison test group 54, and was found to have an excellent distinctive aroma. In 2005, propagation by cutting (nursery selection) was performed. Clonal line comparison testing took place from 2006 to 2012. Local adaptability testing, as well as testing for specific characteristics, was performed from 2011 to 2018.
  • ‘Kiyoka’ was submitted under the name ‘Yachaken 04 Gou’ to local adaptability test group No. 13. Furthermore, in 2014, ‘Kiyoka’ was subjected to cultivation and processing testing as a candidate variety having excellent color and flavor/aroma in a “science and technology research promotion program for agriculture, forestry, fisheries and food industry” (plant variety producing project), specifically a program for “breeding of tea variety suited for commercial user needs, and development of techniques for cultivation, processing, and use thereof”.
  • Plant growth habit is semi upright to spreading. Plant vigor is medium to strong. Plant is of “gajugata” (bud weight) type. Cutting propagation rate is favorable. Data from 2017 local adaptability tests shows plant spread to be somewhat larger than ‘Yabukita’, and somewhat smaller than ‘Saemidori’, and therefore nearly the equivalent to that of parent strains ‘Yabukita’ and ‘Saemidori’.
  • the fruit and seed of ‘Kiyoka’ take a form similar to that taken by the fruit and seed of a common tea tree. Specifically, the fruit is capsular, and most of the fruit is occupied by seed(s). The fruit includes several seeds (ordinarily 1 seed to 3 seeds) that are enclosed by the pericarp and each of which is a grain including the embryo and albumen that are enclosed by a seed coat.
  • the average for fresh leaf yield of ‘Kiyoka’ was, for first flush, less than that of ‘Yabukita’ and ‘Saemidori’, and for the second flush, was equivalent to that of ‘Yabukita’ and less than that of ‘Saemidori’.
  • yield across all tea-plucking seasons was less than that of ‘Saemidori’, but greater than that of ‘Yabukita’.
  • ‘Kiyoka’ also exhibits excellent color, with dark green fresh leaves. SPAD value (an index of chlorophyll content) was higher than in ‘Yabukita’ and equivalent to or higher than in ‘Saemidori’ in all tea-plucking seasons.
  • ‘Kiyoka’ was found to be early budding, with the time of first flush sprouting in Japan being 13 days earlier than for ‘Yabukita’, and time of plucking in Japan being 8 days earlier than for ‘Yabukita’.
  • average data year-on-year cumulative average
  • ‘Kiyoka’ was examined for natural occurrence of anthracnose in the local adaptability tests. It was found that, although the score for degree of occurrence differed between regions, the average score was 2.7, with a maximum of 5.0. This score represented a lower degree of occurrence than in ‘Yabukita’ (3.4), but higher than in ‘Saemidori’ (2.5).
  • an inoculation test and in-field natural occurrence test for anthracnose were carried out. Comprehensively considering the data from both the local adaptability tests and from the breeding site, resistance against anthracnose is deemed to be “weak to medium”.
  • ‘Kiyoka’ was examined for natural occurrence of gray blight in the older version of the local adaptability tests. It was found that the average score for occurrence degree was 1.2, which was less than the average for ‘Yabukita’ (1.7). Furthermore, in an inoculation test at the breeding site, ‘Kiyoka’ was determined to have medium resistance against gray blight. As such, ‘Kiyoka’ is deemed to have practical resistance against gray blight, and fungicide spraying is therefore unnecessary.
  • ‘Kiyoka’ scores for cold injury in winter leaves had a maximum value of 5.0 in Japan, indicating greater damage than in ‘Yabukita’ (4.0).
  • ‘Kiyoka’ was determined to have weak to medium resistance, which is equivalent to the rating for ‘Saemidori’, but lower than Yabukita's resistance (2017 research data by the National Agriculture and Food Research Organization).
  • a cold drought damage of winter leaves is wilting in which leaves wilt due to lack of moisture; leaves maintain a green color but lose gloss.
  • cold drought damage was observed only in Saitama Prefecture in Japan.
  • the score for degree of occurrence of cold drought damage was 5.0 for ‘Kiyoka’, which was equivalent to the score for ‘Yabukita’. As such, there is the risk that cold drought damage will occur in colder regions having a yearly average temperature of 9 to 12 degrees Celsius.
  • ‘Kiyoka’ is an early budding variety for use as sencha (a type of Japanese green tea which is prepared by infusing the processed whole tea leaves in hot water).
  • Suitable cultivation sites for ‘Kiyoka’ are warm regions having a yearly average temperature of 15 to 18 degrees Celsius. When permanently planting ‘Kiyoka’ in moderately warm regions having a yearly average temperature of 12 to 15 degrees Celsius, it is necessary to carefully determine the weather conditions of the tea field, including choosing a location with sufficient sunlight and little frost.
  • ‘Kiyoka’ is an early budding variety, it is necessary to implement anti-frost measures in regions where late frost damage can occur. Anthracnose occurrence is less prevalent than in ‘Yabukita’, but it is preferable to implement fungicide control measures. Pesticide spraying against bacterial shoot blight are necessary in regions where occurrence of bacterial shoot blight is observed.
  • ‘Kiyoka’ had superior processed tea quality at both first flush and second flush, with an aroma having a strong, distinctive floral fragrance, and a superior flavor as well.
  • ‘Kiyoka’ had a higher total nitrogen and free amino acid content than Sofu, and an equivalent tannin content and caffeine content. It is presumed that the higher free amino acid content is reflected in the difference in flavor. Thus, these results indicate that ‘Kiyoka’ is superior to Sofu in terms of both yield and processed tea quality.
  • ‘Kiyoka’ was grown in open culture. In Japan, planting occurs in early February to early April. In Japan cutting time is early June to late June. In Japan, flowering occurs in early October to mid-November, and harvesting time is early April to mid-April.
  • the average size of the plant and tea quality may vary with location, season, nutrition, irrigation, etc.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Plants (AREA)

Abstract

‘Kiyoka’ is a new variety of tea plant bred by crossbreeding FYZ-41 (‘Yabukita’ x ‘Shizuinzatsu 131’), a hybrid, as the female parent and ‘Saemidori’ as the male parent. The steamed leaves and leaf buds of ‘Kiyoka’ have a distinctive floral aroma, and excellent taste.

Description

Plant name and variety denomination:
Latin name of the genus and species of the plant claimed: Camellia sinensis (L.) Kuntze. The claimed plant is a hybridization of different varieties.
Common names of the claimed plant's species: Tea plant.
Variety denomination: ‘Kiyoka’.
BACKGROUND
Camellia sinensis (L.) Kuntze, commonly called tea plant or tea shrub, is an evergreen shrub or small tree whose leaves and leaf buds are used to produce tea. Camellia sinensis is mainly cultivated in tropical and subtropical climates. Camellia sinensis is native to East Asia, the Indian Subcontinent, and Southeast Asia, however it is currently cultivated across the world in tropical and subtropical regions.
BRIEF SUMMARY
‘Kiyoka’ is a new variety of Camellia sinensis (L.) Kuntze bred by crossbreeding FYZ-41 (‘Yabukita’ x ‘Shizuinzatsu 131’), a hybrid, as the female parent and ‘Saemidori’ as the male parent. ‘Kiyoka’ is propagated by grafts or cuttings. The plant may be used, e.g., for cultivation of tea leaves and leaf buds for consumption. ‘Kiyoka’ has been asexually reproduced in Kagoshima prefecture, Japan. ‘Kiyoka’ is propagated by cutting. Cutting is a method in which a young shoot is adjusted to a cutting having one leaf or two leaves, and the cutting is then inserted into soil, or the like, so as to take root.
Approximately 75% of Japan's tea fields are used for cultivating ‘Yabukita’. An adverse effect of such singular cultivation is that Japanese tea lacks diversity of aroma and flavor. Currently, the needs of commercial users and consumers of tea are diversifying, and there is a need for new tea varieties which have their own distinctive aroma and flavor. To date, the tea varieties ‘Sofu’ and ‘Fuji Kaori’ (which are crossbreeds between ‘Yabukita’ and ‘Assam’ variant progeny lines) have been bred as varieties having a distinctive aroma. However, when such teas are used as sencha (a type of Japanese green tea), while they do have an excellent oriental-orchid-like floral fragrance, they also often have an astringent flavor. There has been development of new techniques for withering plucked fresh leaves in a manner that enhances aroma, by utilizing low-temperature moisture-removal withering or new artificial withering devices. Unfortunately, such techniques result in a tea whose color of liquor differs from that of sencha and is closer to that of semi-fermented tea.
‘Kiyoka’ is a variety obtained by selecting individuals from progeny of a crossbreed between (i) FYZ-41 (seed parent), which is an Assam variant crossbreed progeny having a floral fragrance, and (ii) ‘Saemidori’, which is a high-quality variety having strong umami. ‘Kiyoka’ is characterized in that (i) it has a clean and distinctive floral fragrance like that of an oriental orchid, (ii) its color of liquor is greenish, and (iii) it has a flavor exhibiting umami. ‘Kiyoka’ is early budding and thus limited in terms of cultivation region, and its yield is less than that of ‘Saemidori’. However, ‘Kiyoka’ presents the possibility of development of new products which utilize its new characteristic aroma and flavor.
BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 is a breeding pedigree chart for ‘Kiyoka’.
FIG. 2 is a photograph of ‘Kiyoka’ first flush, young shoots.
FIG. 3 is a photograph of the state of ‘Kiyoka’ plants at time of first flush.
FIG. 4 is a photograph of the state of ‘Kiyoka’ plants in the cultivation field at the time of plucking. Ruler shown is 1 meter.
FIG. 5 is a photograph of the state of parent variety ‘Saemidori’ plants in the cultivation field at the time of plucking. Ruler shown is 1 meter.
FIG. 6 is a photograph of the state of grandparent variety ‘Yabukita’ plants in the cultivation field at the time of plucking. Ruler shown is 1 meter.
FIG. 7 is a photograph of the shape of young shoots at plucking (first flush) of ‘Kiyoka’ (left), ‘Saemidori’ (center), and ‘Yabukita’ (right).
FIG. 8 is a photograph of the shape of young leaves from water sprout at plucking (first flush) for ‘Kiyoka’ (left), ‘Yabukita’ (center), and ‘Saemidori’ (right).
 DETAILED BOTANICAL DESCRIPTION OF THE PLANT
Variety: ‘Kiyoka’.
Species of the plant claimed: Camellia sinensis (L.) Kuntze.
Common names of the claimed plant's species include: tea plant or tea shrub.
Characteristics of ‘Kiyoka’ provided herein were observed when the plant was cultivated in Japan.
Properties and characteristics of ‘Kiyoka’, compared to the parent varieties, are described in
Tables 1 and 2 below.
TABLE 1
Properties and characteristics of ‘Kiyoka’ clearly distinguishable
from similar varieties.
Denomination State of State of
of Similar Description of Expression in Expression in
Variety Characteristics Similar Variety ‘Kiyoka’
Yabukita Plant: vigor Weak to medium Medium to
strong
Saemidori Plant: vigor Weak to medium Medium to
strong
Yabukita Plant: growth habit Upright to semi Semi upright to
upright spreading
Yabukita Time of sprouting Medium Early
Yabukita Young shoot: time of
beginning of “one Medium Early
and a bud” stage
Yabukita Time of plucking Medium Early
Yabukita Leaf blade: intensity Medium Light
of green color
Saemidori Leaf blade: intensity Medium Light
of green color
Yabukita Fermentation ability Weak Medium
Saemidori Fermentation ability Weak Medium
TABLE 2
Table of Characteristics: Kiyoka (data from two years; in cases where
characteristic is identical in second year, per-year results are not shown).
Charac- State/score of
teris- expression in
tics Characteristics candidate variety
No. Characteristic note state score data
1 Vigor Plant Medium 6
to
strong
2 Type Plant Shrub 1
3 Growth Plant Semi 4
habit upright
to
spreading
4 Density of Plant Medium 5
branches
5 Branch Plant Absent 1
zigzagging
6 Time of 70% Early 3 Mar. 12,
sprouting of the 2016
plants Mar. 23,
show 2017
sprouts
7 Time of One Early 3 Mar. 23,
beginning and 2016
two Apr. 3,
bud 2017
stage
of
young
shoot
8 Color of the Three Medium 4 140A
third leaf and a green
bud
stage
of
young
shoot
9 Pubescence Young present 9
of bud shoot
10 Density Young Dense 7
of shoot
pubescence
of bud
11 Anthocyanin Young Absent 1
coloration shoot
at base of
the patiole
12 Length of Young Medium 5 5.30 cm
“three and shoot (2016)
a bud” 4.94 cm
(2017)
13 Time of Early 3 Apr. 13,
plucking 2016
Apr. 20,
2017
14 Number of Young Medium 5 938
buds at shoot buds/m2
plucking (2016)
time 1206
buds/m2
(2017)
15 Thickness Medium 5 3.00 mm
of shoot (2016)
3.29 mm
(2017)
16 Leaf Mature Outwards 3
attitude leaf
17 Length of Mature Medium 5 9.23 cm
leaf blade leaf (2016)
9.68 cm
(2017)
18 Width of Mature Medium 5 3.49 cm
leaf blade leaf (2016)
3.66 cm
(2017)
19 Shape of Mature Medium 3
leaf blade leaf elliptic
20 Intensity of Mature Light 3 134A
green color leaf
of leaf blade
21 Shape of Mature Flat 2
cross leaf
section of
leaf blade
22 Texture of Mature Moderately 2
upper leaf rugose
surface of
leaf blade
23 Shape of Mature Acute 2
apex of leaf
leaf blade
24 Undulation Mature Absent or 1
of margin of leaf weak
leaf blade
25 Serration of Mature Medium 5
margin of leaf
leaf blade
26 Shape of Mature Actue 1
base of leaf
leaf blade
27 Time of Flower Medium 5 Oct. 20,
full 2016
flowering Oct. 28,
2017
28 Length of Flower Medium 5 10.2 mm
pedicel (2016)
10.3 mm
(2017)
29 Pubescence Flower Absent 1
on outer
side of sepal
30 Anthocyanin Flower Absent 1
coloration on
sepal on
outer side
31 Diameter Flower Medium 5 3.43 cm
(2016)
3.44 cm
(2017)
32 Color of Flower White 2 There is
innter (Upper & no
lower appro-
surface) priate
color on
the RHS
color
chart.
33 Pubescence Flower Present 9
of ovary
34 Density of Flower Dense 7
pubscence
of ovary
35 Length of Flower Medium 5 9.89 mm
style (2016)
10.29
mm
(2017)
36 Position of Flower Medium 5
style
splitting
37 Position of Flower Same level 3
stigma
38 Fermentation Medium 5
ability
39 Caffeine Medium 3 4.1%
content DW
(2017)
4.5%
DW
(2018)
Additional
Characteristics
Petiole 4.13 mm
length (2020)
Color of 141C
Petiole
Diameter of 7.51 mm
Flower Bud
Shape of spherical
Flower bud shape
Color of white There is
flower bud no
appro-
priate
color on
the RHS
color
chart.
Length of 4.25 mm
Sepal
Shape of triangle
Septal with
rounded
edges
Color of 134B
Sepal
Charac-
teris- State/Score of reference variety
tics Yabukita Saemidori
No. state score data state score data
1 Weak 4 Weak 4
to to
medium medium
2 Shrub 1 Shrub 1
3 upright 2 Semi 3
to semi upright
upright
4 Medium 5 Dense 7
5 Absent 1 Absent 1
6 Medium 5 Mar. 27, Early 3 Mar. 19,
2016 2016
Apr. 5, Mar. 27,
2017 2017
7 Medium 5 Apr. 2, Early 3 Mar. 26,
2016 2016
Apr. 10, Apr. 5,
2017 2017
8 Medium 4 140A Medium 4 140A
green green
9 present 9 present 9
10 Medium 5 Medium 5
11 Absent 1 Absent 1
12 Medium 5 5.43 cm Medium 5 5.71 cm
(2016) (2016)
5.66 cm 4.49 cm
(2017) (2017)
13 Medium 5 Apr. 22, Early 3 Apr. 14,
2016 2016
Apr. 27, Apr. 20,
2017 2017
14 Medium 5 837 Many 7 1425
buds/m2 buds/m2
(2016) (2016)
1238 1662
buds/m2 buds/m2
(2017) (2017)
15 Medium 5 2.72 mm Medium 5 2.78 mm
(2016) (2016)
2.93 mm 3.21 mm
(2017) (2017)
16 Outwards 3 Upwards 2
to
outwards
17 Medium 5 9.54 cm Medium 5 8.99 cm
(2016) (2016)
9.18 cm 8.40 cm
(2017) (2017)
18 Medium 5 3.43 cm Medium 5 3.15 cm
(2016) (2016)
3.53 cm 3.24 cm
(2017) (2017)
19 Narrow 2 Narrow 2
elliptic elliptic
20 Dark 5 135B Dark 5 135B
21 Flat 2 Flat 2
22 Moderately 2 Moderately 2
rugose rugose
23 Acute 2 Acute 2
24 Absent or 1 Absent or 1
weak weak
25 Medium 5 Medium 5
26 Acute 1 Acute 1
27 Medium 5 Oct. 20, Medium 5 Oct. 18,
2016 2016
Oct. 28, Oct. 26,
2017 2017
28 Medium 5 11.4 mm Medium 5 10.7 mm
(2016) (2016)
11.2 mm 10.7 mm
(2017) (2017)
29 Absent 1 Absent 1
30 Absent 1 Absent 1
31 Medium 5 3.60 cm Medium 5 3.49 cm
(2016) (2016)
3.42 cm 3.60 cm
(2017) (2017)
32 White 2 There is White 2 There is
(Upper & no (Upper & no
lower appro- lower appro-
surface) priate surface) priate
color on color on
the RHS the RHS
color color
chart. chart.
33 Present 9 Present 9
34 Dense 7 Dense 7
35 Low to 4 9.33 mm Medium 5 10.33
medium (2016) mm
9.46 mm (2016)
(2017) 10.00
mm
(2017)
36 Medium 5 Medium 5
37 Same level 3 Same level 3
38 weak 3 weak 3
39 Medium 3 3.5% Medium 3 4.0%
DW DW
(2017) (2017)
3.5% 3.9%
DW DW
(2018) (2018)
4.24 mm 4.76 mm
(2020) (2020)
141C 141C
7.06 mm 6.36 mm
spherical spherical
shape shape
white There is white There is
no no
appro- appro-
priate priate
color on color on
the RHS the RHS
color color
chart. chart.
4.33 mm 3.73 mm
triangle triangle
with with
rounded rounded
edges edges
134B 134B
Data of Additional Characteristics were observed in Aug. 17, 2020.
Color scores are according to the Fifth Edition (2007) of ″The Royal Horticultural Society (RHS) Color chart.
Currently, most green tea varieties that are prevalent in Japan are either the ‘Yabukita’ variety (accounting for approximately 75% of tea cultivation land area) or varieties whose aroma resembles ‘Yabukita’. There is a lack of varieties having a strong unique aroma. Typically, for sencha, each tea company produces its own original sencha product by having a tea expert blend a number of varieties, with ‘Yabukita’ serving as the base. Tea varieties having a strong unique aroma of their own tend to ruin the balance between aroma and flavor when blended with ‘Yabukita’. As such, there had previously been little demand for such varieties from commercial users. Currently, however, the needs of commercial users and consumers of tea are diversifying, and there is therefore a demand for new tea varieties which have their own strong, distinctive aroma. In recent times there has been an increase in development of tea products having new flavor/aroma by using existing varieties but modifying processing techniques to enhance the aroma. However, production methods for sencha normally use few varieties having their own strong, distinctive aroma. Furthermore, varieties that do have a characteristic aroma often have an astringent flavor. As such, the aim of the present tea variety is to breed a green tea variety which differs from conventional tea varieties by having a strong, distinctive aroma and excellent flavor.
Cross-breeding of ‘Kiyoka’ was carried out in 1998 at Makurazaki-shi in Kagoshima, Japan. Seed collection, sowing, and cultivation was performed in 1999. In 1998, an F1 seedling group was obtained by crossbreeding (i) FYZ-41 (seed parent), which has a strong floral fragrance, and (ii) ‘Saemidori’ (pollen parent), which is early maturing and provides excellent processed tea quality. ‘FYZ-41’ is a line that has not been established as a variety. In breeding tea, such a line may be used as a hybrid parent in cases where specific characteristic data concerning the line is not completely available but the line only has target characteristics. ‘FYZ-41’ was used as a female parent because it had the target characteristic scent of flowers, however specific characteristic data concerning ‘FYZ-41’ is not available. ‘Kiyoka’ was selected from this F1 seedling group in an individual selection test in 2005 (see FIG. 1). Individual selection tests were performed from 2001 to 2005. Thereafter, ‘Kiyoka’ was submitted under the name ‘Makurazaki 54-20’ to clonal line comparison test group 54, and was found to have an excellent distinctive aroma. In 2005, propagation by cutting (nursery selection) was performed. Clonal line comparison testing took place from 2006 to 2012. Local adaptability testing, as well as testing for specific characteristics, was performed from 2011 to 2018. In 2011, ‘Kiyoka’ was submitted under the name ‘Yachaken 04 Gou’ to local adaptability test group No. 13. Furthermore, in 2014, ‘Kiyoka’ was subjected to cultivation and processing testing as a candidate variety having excellent color and flavor/aroma in a “science and technology research promotion program for agriculture, forestry, fisheries and food industry” (plant variety producing project), specifically a program for “breeding of tea variety suited for commercial user needs, and development of techniques for cultivation, processing, and use thereof”.
Morphological Characteristic
Plant growth habit is semi upright to spreading. Plant vigor is medium to strong. Plant is of “gajugata” (bud weight) type. Cutting propagation rate is favorable. Data from 2017 local adaptability tests shows plant spread to be somewhat larger than ‘Yabukita’, and somewhat smaller than ‘Saemidori’, and therefore nearly the equivalent to that of parent strains ‘Yabukita’ and ‘Saemidori’. The fruit and seed of ‘Kiyoka’ take a form similar to that taken by the fruit and seed of a common tea tree. Specifically, the fruit is capsular, and most of the fruit is occupied by seed(s). The fruit includes several seeds (ordinarily 1 seed to 3 seeds) that are enclosed by the pericarp and each of which is a grain including the embryo and albumen that are enclosed by a seed coat.
In local adaptability tests, the average for fresh leaf yield of ‘Kiyoka’ was, for first flush, less than that of ‘Yabukita’ and ‘Saemidori’, and for the second flush, was equivalent to that of ‘Yabukita’ and less than that of ‘Saemidori’. At the breeding site, yield across all tea-plucking seasons (first flush to third flush) was less than that of ‘Saemidori’, but greater than that of ‘Yabukita’. ‘Kiyoka’ also exhibits excellent color, with dark green fresh leaves. SPAD value (an index of chlorophyll content) was higher than in ‘Yabukita’ and equivalent to or higher than in ‘Saemidori’ in all tea-plucking seasons.
Additional properties and characteristics of ‘Kiyoka’, which may serve as useful reference in variety examination, are described in Table 3.
TABLE 3
Additional properties and characteristics of ‘Kiyoka’.
Denomination State of State of
of Similar Description of Expression in Expression in
Variety Characteristics Similar Variety ‘Kiyoka’
Yabukita Blister blight Weak to Medium to
resistance medium strong
Yabukita Gray blight resistance Weak Medium
Saemidori Gray blight resistance Weak Medium
Saemidori Anthracnose resistance Medium Weak to
medium
Ecological Characteristics
‘Kiyoka’ was found to be early budding, with the time of first flush sprouting in Japan being 13 days earlier than for ‘Yabukita’, and time of plucking in Japan being 8 days earlier than for ‘Yabukita’. In the local adaptability tests, average data (year-on-year cumulative average) showed ‘Kiyoka’ time of sprouting being 4 days earlier than for ‘Yabukita’ and time of plucking being 2 days earlier than for Yabukita.
The field resistance of ‘Kiyoka’ to natural occurrence of anthracnose in autumn was deemed to be “weak to medium”. In inoculation tests, resistance to anthracnose was similarly found to be “weak to medium”. As such, ‘Kiyoka’ was deemed to have anthracnose resistance which is, overall, “weak to medium”. Gray blight (Discula theae-sinensis) resistance was “medium”. Resistance against both anthracnose and gray blight was slightly stronger than in ‘Yabukita’. Resistance to bacterial shoot blight in field inoculation tests was found to be “weak”. Blister blight resistance was determined to be “medium to strong” in tests for specific characteristics. Resistance to white peach scale (Pseudaulacaspis pentagona) was determined to be “weak”, with degree of occurrence being equivalent to that of Yabukita.
‘Kiyoka’ was examined for natural occurrence of anthracnose in the local adaptability tests. It was found that, although the score for degree of occurrence differed between regions, the average score was 2.7, with a maximum of 5.0. This score represented a lower degree of occurrence than in ‘Yabukita’ (3.4), but higher than in ‘Saemidori’ (2.5). At the breeding site, an inoculation test and in-field natural occurrence test for anthracnose were carried out. Comprehensively considering the data from both the local adaptability tests and from the breeding site, resistance against anthracnose is deemed to be “weak to medium”.
‘Kiyoka’ was examined for natural occurrence of gray blight in the older version of the local adaptability tests. It was found that the average score for occurrence degree was 1.2, which was less than the average for ‘Yabukita’ (1.7). Furthermore, in an inoculation test at the breeding site, ‘Kiyoka’ was determined to have medium resistance against gray blight. As such, ‘Kiyoka’ is deemed to have practical resistance against gray blight, and fungicide spraying is therefore unnecessary.
In the local adaptability tests, occurrence of bacterial shoot blight (Pseudomonas syringae pv. theae) in ‘Kiyoka’ was observed only in Shizuoka Prefecture in Japan. At Shizuoka, the degree of occurrence was higher than in both ‘Yabukita’ and ‘Saemidori’. An in-field inoculation test for bacterial shoot blight was carried out at the breeding site, and it was found that for ‘Kiyoka’, the number of symptomatic leaves was less compared to ‘Yabukita’, but somewhat more compared to ‘Saemidori’. In 2015 research data by the NARO Institute of Vegetable and Tea Science, ‘Yabukita’ and ‘Saemidori’ were determined to have weak resistance against bacterial shoot blight. In view of this data, ‘Kiyoka’ was also determined to have weak resistance against bacterial shoot blight.
A field resistant test for blister blight (Exobasidium vexans) was carried out in Shizuoka Prefecture in Japan. It was found that in ‘Kiyoka’, the degree of blister blight occurrence was consistently lower than that of the comparative variety ‘Okuhikari’, and thus ‘Kiyoka’ was deemed to have medium to strong resistance.
In the older version of the local adaptability tests, the average score for occurrence of Pseudaulacaspis pentagona was 3.4, with a maximum score of 5.0. The degree of occurrence in ‘Kiyoka’ was equivalent to that of ‘Yabukita’ and ‘Saemidori’, which are both considered to have weak resistance. ‘Kiyoka’ resistance to Pseudaulacaspis pentagona was therefore determined to be weak.
With regard to the resistance of ‘Kiyoka’ to cold, resistance to cold injury in winter leaves (wilting in which leaves turn reddish-brown due to cold temperatures) was found to be “medium to weak”, which is lower than that of ‘Yabukita’ but equivalent to that of ‘Saemidori’. Resistance to bark splitting frost damage was “medium,” which is equivalent to that of Yabukita.
In the older version of the local adaptability tests, the ‘Kiyoka’ scores for cold injury in winter leaves had a maximum value of 5.0 in Japan, indicating greater damage than in ‘Yabukita’ (4.0). In a cold injury resistance test at the breeding site, ‘Kiyoka’ was determined to have weak to medium resistance, which is equivalent to the rating for ‘Saemidori’, but lower than Yabukita's resistance (2017 research data by the National Agriculture and Food Research Organization).
A cold drought damage of winter leaves is wilting in which leaves wilt due to lack of moisture; leaves maintain a green color but lose gloss. In local adaptability tests, cold drought damage was observed only in Saitama Prefecture in Japan. The score for degree of occurrence of cold drought damage was 5.0 for ‘Kiyoka’, which was equivalent to the score for ‘Yabukita’. As such, there is the risk that cold drought damage will occur in colder regions having a yearly average temperature of 9 to 12 degrees Celsius.
A test for specific characteristics regarding resistance to bark splitting frost damage was carried out in Kagoshima Prefecture in Japan. As a result, ‘Kiyoka’ was found to have medium resistance, which was equivalent to the rating for ‘Yabukita’.
Quality Characteristics and Processability
In standard cultivation, ‘Kiyoka’ is an early budding variety for use as sencha (a type of Japanese green tea which is prepared by infusing the processed whole tea leaves in hot water). Suitable cultivation sites for ‘Kiyoka’ are warm regions having a yearly average temperature of 15 to 18 degrees Celsius. When permanently planting ‘Kiyoka’ in moderately warm regions having a yearly average temperature of 12 to 15 degrees Celsius, it is necessary to carefully determine the weather conditions of the tea field, including choosing a location with sufficient sunlight and little frost.
Because ‘Kiyoka’ is an early budding variety, it is necessary to implement anti-frost measures in regions where late frost damage can occur. Anthracnose occurrence is less prevalent than in ‘Yabukita’, but it is preferable to implement fungicide control measures. Pesticide spraying against bacterial shoot blight are necessary in regions where occurrence of bacterial shoot blight is observed.
In local adaptability tests, the average for first flush processed tea quality of ‘Kiyoka’ was equivalent to that of ‘Yabukita’, and lower than that of ‘Saemidori’. The average for second flush processed tea quality was somewhat better than that of both ‘Yabukita’ and ‘Saemidori’. This is presumably because the aroma and flavor of ‘Kiyoka’ have a floral fragrance unique to the variety, and thus when ‘Kiyoka’ was tested as sencha, depending on the test location, it was deemed to have flavor and aroma which were of a different nature to sencha. Note that at the breeding site, ‘Kiyoka’ was found to have processed tea quality which exceeded that of both ‘Saemidori’ and ‘Yabukita’ in all tea-plucking seasons, and was found to have a distinctive floral fragrance.
Plant Growth
Cultivation characteristics, processed tea quality, and chemical composition were examined for ‘Kiyoka’ and ‘Sofu’, which is an existing early budding variety that has an oriental-orchid-like floral fragrance. The two varieties are similar in terms of plant growth habit, plant vigor, time of sprouting, and date of plucking, but ‘Kiyoka’ has a higher fresh leaf yield and higher number of buds per square meter. Upon evaluation of SPAD value (which indicates chlorophyll content), it was found that Sofu had a higher SPAD value at first flush, whereas ‘Kiyoka’ had a higher SPAD value at second flush. However, regarding processed tea quality, it was found that ‘Kiyoka’ had superior color of processed tea at both first flush and second flush. ‘Kiyoka’ had superior processed tea quality at both first flush and second flush, with an aroma having a strong, distinctive floral fragrance, and a superior flavor as well. In a comparison of chemical composition, it was found that at both first flush and second flush, ‘Kiyoka’ had a higher total nitrogen and free amino acid content than Sofu, and an equivalent tannin content and caffeine content. It is presumed that the higher free amino acid content is reflected in the difference in flavor. Thus, these results indicate that ‘Kiyoka’ is superior to Sofu in terms of both yield and processed tea quality.
In the local adaptability tests, regarding the chemical composition of ‘Kiyoka’ across all tea-plucking seasons, total nitrogen content and free amino acid content was higher than in ‘Yabukita’ in some test sites, but somewhat lower than ‘Saemidori’. Tannin content was equivalent to or slightly lower than in ‘Yabukita’, and slightly higher than in ‘Saemidori’. At the breeding site, across all tea-plucking seasons, total nitrogen content and free amino acid content in ‘Kiyoka’ was higher than in ‘Yabukita’, and nearly equivalent to that of ‘Saemidori’.
Other Characteristics
At the breeding site, during all tea-plucking seasons (first flush through third flush), yield of ‘Kiyoka’ was greater than that of ‘Yabukita’ and somewhat less than that of ‘Saemidori’. In all tea-plucking seasons (first flush through third flush), processed tea quality was superior to that of ‘Saemidori’, which is a high-quality variety. The aroma of ‘Kiyoka’ was found to have a distinctive floral fragrance. In the local adaptability tests, average values for both first flush yield and second flush yield of ‘Kiyoka’ were less than those of ‘Saemidori’. First flush yield was less than that of ‘Yabukita’, but second flush yield was greater than that of ‘Yabukita’. The average values for processed tea quality of both first flush and second flush of ‘Kiyoka’ were equivalent to those for ‘Yabukita’, and slightly lower than those for ‘Saemidori’.
‘Kiyoka’ was grown in open culture. In Japan, planting occurs in early February to early April. In Japan cutting time is early June to late June. In Japan, flowering occurs in early October to mid-November, and harvesting time is early April to mid-April.
It will be understood that the average size of the plant and tea quality may vary with location, season, nutrition, irrigation, etc.

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1. A new and distinct Camellia sinensis (L.) Kuntze plant named ‘Kiyoka’ as illustrated and described herein.
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