USPP19793P3 - Loblolly pine tree named ‘CF Q7766’ - Google Patents
Loblolly pine tree named ‘CF Q7766’ Download PDFInfo
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- USPP19793P3 USPP19793P3 US11/635,199 US63519906V USPP19793P3 US PP19793 P3 USPP19793 P3 US PP19793P3 US 63519906 V US63519906 V US 63519906V US PP19793 P3 USPP19793 P3 US PP19793P3
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- loblolly pine
- pttx
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- tree
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- 241000218679 Pinus taeda Species 0.000 title claims abstract description 16
- 235000008566 Pinus taeda Nutrition 0.000 title claims abstract description 15
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 abstract description 9
- 108700028369 Alleles Proteins 0.000 description 11
- 238000005259 measurement Methods 0.000 description 8
- 208000015181 infectious disease Diseases 0.000 description 7
- 108091092878 Microsatellite Proteins 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 230000002068 genetic effect Effects 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- 230000030118 somatic embryogenesis Effects 0.000 description 4
- 108020004414 DNA Proteins 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- BHELIUBJHYAEDK-OAIUPTLZSA-N Aspoxicillin Chemical compound C1([C@H](C(=O)N[C@@H]2C(N3[C@H](C(C)(C)S[C@@H]32)C(O)=O)=O)NC(=O)[C@H](N)CC(=O)NC)=CC=C(O)C=C1 BHELIUBJHYAEDK-OAIUPTLZSA-N 0.000 description 2
- 241000218631 Coniferophyta Species 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 210000000481 breast Anatomy 0.000 description 2
- 210000002257 embryonic structure Anatomy 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 230000000392 somatic effect Effects 0.000 description 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- 241000864211 Cronartium quercuum Species 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- 238000007399 DNA isolation Methods 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000230267 Fusarium circinatum Species 0.000 description 1
- 108091036333 Rapid DNA Proteins 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000011681 asexual reproduction Effects 0.000 description 1
- 238000013465 asexual reproduction Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000010154 cross-pollination Effects 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000408 embryogenic effect Effects 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 235000017807 phytochemicals Nutrition 0.000 description 1
- 229930000223 plant secondary metabolite Natural products 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 102000054765 polymorphisms of proteins Human genes 0.000 description 1
- 230000002250 progressing effect Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 238000013138 pruning Methods 0.000 description 1
- 239000001044 red dye Substances 0.000 description 1
- 238000005204 segregation Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H7/00—Gymnosperms, e.g. conifers
Definitions
- This new variety is a progeny of a second generation selection pollinated by a first generation selection.
- Female parent is an open pollinated progeny of Georgetown County, S.C. first generation selection.
- Male parent is a first generation selection made in Barnwell County S.C.
- a new and distinct cultivar of loblolly pine ( Pinus taeda ) is distinctly characterized by great resistance to fusiform rust and pitch canker, exceptionally high growth rate, excellent stem straightness, distinctive very long internodes, narrow crown, and which is mature for commercial harvesting sooner than conventionally grown trees under the ecological conditions prevailing in the Piedmont, Atlantic and Gulf Coastal Plains, and Mid-Continent regions of the United States.
- Somatic embryogenesis uses a complex process which relies on the splitting of one embryo into many identical embryos. Somatic embryos can then be grown into plants which are all identical genetically. The asexual propagation occurs at an earlier stage in the plant's life cycle than most other micropropagated plants.
- the detailed methods for somatic embryogenesis used for asexually propagating conifers in general are described in U.S. Pat. No. 6,372,496 and for loblolly pine in particular in U.S. patent application 2004/0203150.
- the drawings are color photographs showing the new variety of loblolly pine including the long internode length.
- FIG. 1 is a photograph showing ‘CF Q7766’ ramet # 7 planted in Holt, Ga. The picture was taken after five field growing seasons. The picture shows distinctive long stem sections of clearwood between the whorls (long internodes), small to medium branch size (relative to the stem size), flat to medium angle between the branches and the stem and great stem straightness.
- FIG. 2 is a photograph showing ‘CF Q7766’ ramet # 4 planted in Winokur, Ga. The picture was taken after five field growing seasons. The picture shows superiority of growth, narrow tree crown relative to the size of this tree and the size of surrounding neighbors, and distinctive long internodes seen particularly well in the upper crown.
- ‘CF Q7766’ is characterized by exceptionally high growth rate, great resistance to fusiform rust (caused by Cronartium quercuum (Berk.) Miyabe ex Shirai f. sp. fusiforme (Cumm.) Burds. et Snow), great resistance to pitch canker (caused by Fusarium circinatum Nirenburg et O'Donnell), distinctive very long internodes, excellent stem straightness, narrow crown, small to medium branch diameter, flat to medium branch angle and low incidence of forking.
- Microsatellite markers were used to generate a unique DNA fingerprint for the variety. Vegetative buds and/or foliar material from eight individuals each produced by controlled crossing among parents for DNA fingerprinting. The DNA extraction protocol of Doyle and Doyle (1987) was used after slight modifications. DNA fingerprinting of parents and their offspring was initially conducted using a set of nine microsatellite markers (Auckland et. al. 2002) and a final set of five primer pairs were selected for the two lines mentioned above (see Table 1, for sequences and conditions of SSR primers). Primer selection was based on their ability to produce unique alleles and the presence of a high level of polymorphism.
- Microsatellite products were detected by M13 tailed primer (Oetting et al., 1995) or infra-red dye (IRD)-labeled primer.
- the amplification products were electrophoresed on 5.5% Long Ranger polyacrylamide gels using a LiCor 4200 automated sequencer (LiCor Inc., Lincoln, Nebr.). For each family, the female and male parents, as well as eight offspring were genotyped.
- offspring genotypes segregated following expected simple Mendelian segregation (see Table 3, for offspring multi-locus genotypes).
Abstract
Description
- Parentage: Female parent: (Unknown) open pollinated progeny of a first generation selection of Georgetown County, S.C. Male parent: (Unknown) first generation selection of Barnwell County, S.C.
- Disease resistance:
-
- Percent stem fusiform rust infection at age 5.—0.
- Percent branch fusiform rust infection at age 5.—5.
- Percent branch and stem fusiform rust infection at age 5.—0.
- Percent dead ramets due to fusiform rust infection at age 5.—0.
- Percent stem fusiform rust infection in the USDA Resistance Screening Center (Asheville, N.C.) tests after artificial inoculation with rust spores.—5% (compared to 78% infection in unimproved seedlings).
- Percent of seedlings with over 50% of tissue infected by pitch canker in the USDA Resistance Screening Center (Asheville, N.C.) tests after artificial inoculation with pitch canker spores.—1% (compared to 99% infection in unimproved seedlings).
-
- Botanical description:
-
- Shape.—A medium to large tree may reach well over 100 feet tall, self-prunes well and develops a fairly straight trunk and an oval, somewhat open crown.
- Average height.—28 ft after 5 field growing seasons.
- Maximum height.—34 ft after 5 field growing seasons.
- Average trunk diameter at breast height (4.5 feet above the soil level).—4.7 inches after 5 field growing seasons, 6.7 inches to 7.1 inches after seven field growing seasons.
- Maximum trunk diameter at breast height (4.5 feet above the soil level).—6.4 inches after 5 field growing seasons.
- Percent stem forking at age 4.—2.
- Percent stem straightness gain over unimproved trees.—15.
- Bark.—Initially red- to gray-brown and scaly; older trees are ridged and furrowed, with somewhat rounded scaly plates. The RHS color is 200C in shade and 187D on exposure to sun.
- Branch.—Orange-brown in color, fine to moderately stout; buds are narrowly ovoid, light reddish brown.
- Percent ramets with ramicorn branches at age 4.—2.
- Sampling of branch characteristics.—In order to sample branches from a consistent position from one tree to the next the following methodology was utilized. From a point nine feet from the base of the tree, the first complete whorl of limbs below was labeled “Whorl One” and the first complete whorl of limbs above labeled “Whorl Two”. This sampling point was chosen because it is the midpoint of the basal sixteen foot log of each tree. A complete whorl was defined as one with at least three branches. An average whorl contained 2-7 branches. All measurements were taken commencing on the South side of the tree and progressing anticlockwise around the stem. When more than three branches were available for measurement on the whorl the largest three branches, by basal diameter, were used for sampling. The following branch characteristics were measured after seven field growing seasons.
- Branch diameter.—Diameter of each measured branch was taken at its base. Using a caliper the diameter of the branch, over bark, was measured to the closest 100th of an inch. ‘CF Q7766’ has an average branch diameter of 1.00 inches at the base of the branch. Zygotic seedlings of the same genetic origin have an average branch diameter of 1.24 inches at the base of the branch.
- Branch Angle.—Utilizing a large protractor, the angle of each branch was measured as its deviation from horizontal. Branch angles were recorded for the portion of the branch emerging from the stem of the tree with data rounded to the closest 10 degrees. ‘CF Q7766’ has an average branch angle of 35.0 degrees from horizontal. Zygotic seedlings of the same genetic origin have an average branch angle of 43.3 degrees from horizontal.
- Branch length.—The length of each sampled branch was measured directly with a graduated measurement pole. Branch lengths were recorded to the closest 0.5 feet. ‘CF Q7766’ has an average branch length of 6.48 feet. Zygotic seedlings of the same genetic origin have an average branch length of 9.17 feet.
- Crown diameter.—The width of the crown, at the point where branch measurements were taken, was directly measured with the use of a graduated measurement pole. A radial measurement was taken on the East and West side of each tree. Crown radius was measured to the closest 0.5 feet. Crown width data is presented as diameter of the crown. ‘CF Q7766’ has an average crown diameter of 7.8 feet. Zygotic seedlings of the same genetic origin have an average crown diameter of 11.5 feet.
- Internode length.—In proximity to the area of the stem utilized for branch measurements the mean internode length was determined for each tree. Internode distances for the calculation of the mean were directly measured from the stem of the tree using a graduated measurement pole. ‘CF Q7766’ has an average internode length of 3.12 feet. Zygotic seedlings of the same genetic origin have an average internode length of 2.25 feet.
- Leaf.—Evergreen needles, 6 to 9 inches long, with (usually) three yellow-green needles per fascicle. The color of the foliage was measured at age 1 year and age 7 years and was found not to vary significantly with age. The color of the foliage was RHS 137A (40%) and 137C (60%). Diameter of the fascicle was 6/100 of an inch and the average sheath length is 6.75 mm.
- Flower.—Monoecious; males long cylindrical, red to yellow, in clusters at branch tips; females yellow to purple.
- Fruit.—Ovoid to cylindrical, 3 to 6 inch red-brown cones; umbo is armed with a short spine, maturing in early fall. Cones are sporadic in 5-7 year old plants.
- Propagation.—Propagated by somatic embryogenesis.
- Seeds.—none produced yet at 5-7 years of age, plants are not yet mature. Expected seed production by 12-15 years.
- Use.—High yield industrial plantations
-
TABLE 1 |
Sequences and conditions of SSR primers |
currently used in loblolly pine. |
LABEL | |||||
TAIL | |||||
(F/R); | |||||
Primer | E (end | MgCl2 | Tm | Size | |
ID | SEQUENCE ( 5′-3′) | labeled) | (mM) | (° C.) | (bp) |
PtTX | F: CACGACGTTGTAAAAC | F | 2.5 | 59 | 200 |
2146 | GACCTGGGGATTTGGATT | ||||
GGGTATTTG; SEQ ID | |||||
NO: 1 | |||||
R: ATATTTTCCTTGCCCC | |||||
TTCCAGACA; SEQ ID | |||||
NO: 2 | |||||
PtTX | F: CACGACGTTGTAAAAC | F | 1.5 | 55 | 225 |
3034 | GACTCAAAATGCAAAAG | ||||
ACG; SEQ ID NO: 3 | |||||
R: ATTAGGACTGGGGATG | |||||
AT; SEQ ID NO: 4 | |||||
PtTX | F: GAAGTGATAATGGCAT | R | 3 | 55 | 330 |
3049 | AGCAAAAT; SEQ ID NO: 5 | ||||
R: GCAGACCCGTGAAAGT | |||||
AATAAACAT; | |||||
SEQ ID NO: 6 | |||||
PtTX | F: TGTCGGTGGAGTTGGC | E | 2 | 59 | 280 |
3105 | AGTAGACT; SEQ ID NO: 7 | ||||
R: GCCCAGCGTTTCCTG; | |||||
SEQ ID NO: 8 | |||||
PtTX | F: CACGACGTTGTAAAAC | F | 2.5 | 59 | 165 |
3116 | GACCTCCCAAAGCCTAAA | ||||
GAAT; SEQ ID NO: 9 | |||||
R: CATACAAGGCCTTATC | |||||
TTACAGAA; | |||||
SEQ ID NO: 10 | |||||
TABLE 2 |
Parental genotypes and their expected offspring's genotypes |
at five different SSR loci of each family. |
Genotype |
Primer | Female | Male | Expected offspring genotype |
PtTX | 181/199 | 193/208 | 181/193 | 181/208 | 193/199 | 199/208 |
2146 | ||||||
PtTX | 166/184 | 169/172 | 166/169 | 166/172 | 169/184 | 172/184 |
3105 | ||||||
PtTX | 226/228 | 216/226 | 216/226 | 226/226 | 226/228 | |
3034 | ||||||
PtTX | 301/303 | 301/311 | 301/301 | 301/303 | 301/311 | |
3049 | ||||||
PtTX | 151/163 | 151/160 | 151/151 | 151/160 | 160/163 | |
3116 | ||||||
TABLE 3 |
Parents and offspring genotypes at 5 different SSR loci |
for two loblolly pine full-sib families. |
Sample | PtTX 3034a | PtTX 3049a | PtTX 3116 |
ID | ID | Allele 1 | Allele 2 | Allele 1 | Allele 2 | Allele 1 |
Offspring | Q 7766 | 226 | 226 | 301 | 303 | 151 |
PtTX 3116 | PtTX 2146a | PtTX 3105 |
ID | Allele 2 | Allele 1 | Allele 2 | Allele 1 | Allele 2 | ||
Offspring | 151 | 181 | 193 | 169 | 184 | ||
aAllelic sizes have LiCor primer tails. |
- References:
Auckland, L., T. Bui, Y. Zhou, M. Shepherd and C. Williams. 2002. Conifer Microsatellite Handbook Corporate Press, Raleigh, N.C., USA.
Doyle, J. J. and J. L. Doyle. 1987. A rapid DNA isolation procedure for small quantities of fresh tissue. Phytochemical bulletin 19:11-15.
Oetting, W. S., H. K. Lee, D. J. Flanders, G. L. Wiesner, T. A. Sellers and R. A. King. 1995. Linkage analysis with multiplexed short tandem repeat polymorphisms using infrared florescence and M13 tailed primers. Genomics 30:450-458.
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US11/635,199 USPP19793P3 (en) | 2006-12-07 | 2006-12-07 | Loblolly pine tree named ‘CF Q7766’ |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US11/635,199 USPP19793P3 (en) | 2006-12-07 | 2006-12-07 | Loblolly pine tree named ‘CF Q7766’ |
Publications (2)
Publication Number | Publication Date |
---|---|
US20080141406P1 US20080141406P1 (en) | 2008-06-12 |
USPP19793P3 true USPP19793P3 (en) | 2009-03-03 |
Family
ID=39499939
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US11/635,199 Active USPP19793P3 (en) | 2006-12-07 | 2006-12-07 | Loblolly pine tree named ‘CF Q7766’ |
Country Status (1)
Country | Link |
---|---|
US (1) | USPP19793P3 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20100325765P1 (en) * | 2009-06-23 | 2010-12-23 | John Pait | Loblolly pine tree named cf lp1-7696 |
-
2006
- 2006-12-07 US US11/635,199 patent/USPP19793P3/en active Active
Non-Patent Citations (4)
Title |
---|
http://web.archive.org/web/20051130221944.http://www.chesapeakebay.net/info/liblolly.cfm (2 pages). * |
http://www.cnr.vt.edu/DENDRO/dendrology/syllabus/factsheet.cfm (1 page). * |
U.S. Appl. No. 11/635,703, Pait et al., filed Dec. 7, 2006. |
United States Patent Office Action for U.S. Appl. No. 11/635,703 dated Jan. 18, 2008. |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20100325765P1 (en) * | 2009-06-23 | 2010-12-23 | John Pait | Loblolly pine tree named cf lp1-7696 |
USPP21973P3 (en) | 2009-06-23 | 2011-06-14 | Cellfor Inc. | Loblolly pine tree named ‘CF LP1-7696’ |
Also Published As
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US20080141406P1 (en) | 2008-06-12 |
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