USPP11466P - Zoysia grass plant named `SS-500` - Google Patents

Zoysia grass plant named `SS-500` Download PDF

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USPP11466P
USPP11466P US09/031,492 US3149298V US11466P US PP11466 P USPP11466 P US PP11466P US 3149298 V US3149298 V US 3149298V US 11466 P US11466 P US 11466P
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zoysia
grass
toro
color
dna
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Minoru Ito
Roberto Guerra Amaral Gurgel
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Sod Solutions Inc
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12—Leaves
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/469—Zoysia

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  • the present invention relates to a new and distinct variety of a perennial Zoysia grass, discovered on a Brazilian turf farm in Sao Paolo during a routine field inspection.
  • the grass was found in a production field of a common Brazilian grass known as ⁇ Wild Zoysia ⁇ (unpatented).
  • the new grass is believed to be a sport of ⁇ Wild Zoysia ⁇ due to its color and its larger leaf size and longer node length compared to ⁇ Wild Zoysia ⁇ .
  • the novel grass, termed ⁇ SS-500 ⁇ was propagated asexually in Sao Paolo, Brazil.
  • ⁇ SS-500 ⁇ is a distinct, asexually propagated variety of Zoysia grass.
  • ⁇ SS-500 ⁇ is the varietal designation of this new grass.
  • the name ⁇ SS-500 ⁇ may also designate this plant in commerce.
  • ⁇ SS-500 ⁇ is a attractive green Zoysia grass, with an open growth habit. It is characterized by the combination of its color, open growth habit, and large leaf blade length and width.
  • FIG. 1 compares stolons of ⁇ SS-500 ⁇ and ⁇ SS-300 ⁇ .
  • ⁇ SS-300 ⁇ is another new variety of Zoysia grass, and is the subject of co-pending U.S. Plant Patent application Ser. No. 09/028,031.
  • a metric ruler is provided for scale.
  • FIG. 2 compares stolons of ⁇ SS-500 ⁇ and Zoysia cultivars ⁇ Meyer ⁇ , ⁇ El Toro ⁇ , and ⁇ SS-300 ⁇ , next to a metric scale.
  • FIG. 3 shows a runner of ⁇ SS-500 ⁇ that grew to over 30 inches in less than a month.
  • FIG. 4 shows a plug of ⁇ SS-500 ⁇ .
  • FIG. 5 shows ⁇ SS-500 ⁇ in the field at three months. Compare this to FIG. 6.
  • FIG. 6 shows ⁇ El Toro ⁇ in the field at three months, grown under the same conditions as the ⁇ SS-500 ⁇ depicted in FIG. 5.
  • FIG. 7 shows the seedhead of ⁇ SS-500 ⁇ .
  • FIG. 8 shows one view of the root structure of ⁇ SS-500 ⁇ .
  • FIG. 9 shows one view of the root structure of ⁇ SS-500 ⁇ .
  • FIG. 10 provides a comparison of the ⁇ SS-500 ⁇ (top) and ⁇ SS-300 ⁇ (bottom) cultivars.
  • FIG. 11 shows a close-up of stem color of ⁇ SS-500 ⁇ .
  • FIG. 12 is a gel showing DNA fingerprint analysis of ⁇ SS-500 ⁇ in comparison to other varieties of zoyziagrass using primer 8.6i.
  • M molecular size markers in base pairs. Areas of distinct DNA amplification differences are indicated by small arrows.
  • FIG. 13 is a gel showing DNA fingerprint analysis of ⁇ SS-500 ⁇ in comparison to other varieties of zoyziagrass using primer 10.6e.
  • M molecular size markers in base pairs. Areas of distinct DNA amplification differences are indicated by small arrows.
  • ⁇ SS-500 ⁇ is a perennial, vegetatively propagated Zoysia grass, believed to be a variety of Zoysia japonica.
  • the parent of ⁇ SS-500 ⁇ is believed to be a native Brazilian variety of Zoysia japonica, commonly known as ⁇ Wild Zoysia ⁇ .
  • ⁇ SS-500 ⁇ is a wide-bladed grass with an open growth habit.
  • Applicants have asexually propagated ⁇ SS-500 ⁇ by means of stolons and rhizomes. Applicants have discovered the novelty and distinctness of ⁇ SS-500 ⁇ compared to other varieties of Zoysia.
  • ⁇ SS-500 ⁇ has an open growth habit, large stolons and leaf blades, and a massive root system.
  • Side-by-side comparisons of ⁇ SS-500 ⁇ to variety ⁇ El Toro ⁇ demonstrated that ⁇ SS-500 ⁇ exhibits rapid growth; complete coverage can occur within five to six months after planting, compared to ⁇ El Toro ⁇ which takes nine to ten months under similar conditions.
  • Each variety was planted in 8' ⁇ 8' plots using 4-inch prerooted grass plugs planted 12-inches on center to determine coverage rates.
  • ⁇ SS-500 ⁇ is characterized by its large leaf blade width and length, open growth habit and large stolon size, and other characteristics described herein. ⁇ SS-500 ⁇ has a rapid establishment rate, high tolerance to drought, good fall color retention rates, and good tolerance to herbicides MSMA and 2-4-D.
  • ⁇ SS-500 ⁇ was discovered on a Brazilian turf farm in a production field of a common Brazilian grass known as ⁇ Wild Zoysia ⁇ .
  • the new grass was initially believed to be an ⁇ off-type ⁇ , or sport, of ⁇ Wild Zoysia ⁇ due to its color and its larger leaf size (compared to ⁇ Wild Zoysia ⁇ ).
  • ⁇ SS-500 ⁇ exhibits an open growth habit and large leaf width and length, compared to other Zoysia grasses.
  • ⁇ SS-500 ⁇ is low growing during the initial stage of coverage. After the ground is covered the grass becomes upright and open in habit. The grass spreads by stolons and rhizomes that form an open uniform groundcover.
  • the root structure of ⁇ SS-500 ⁇ is massive and deep, with large individual roots.
  • ⁇ SS-500 ⁇ The leaf blade width and length of ⁇ SS-500 ⁇ are larger than ⁇ El Toro ⁇ or other comparable types of Zoysia.
  • ⁇ SS-500 ⁇ has a more open growth habit, fewer seedheads, and longer as well as wider leaf blades than ⁇ Wild Zoysia ⁇ , ⁇ El Toro ⁇ Zoysia (U.S. Plant Pat. No. 5,845), or ⁇ Meyer ⁇ (unpatented) Zoysia.
  • Further comparison testing to variety ⁇ El Toro ⁇ a standard for rapid growth of Zoysia, demonstrates that ⁇ SS-500 ⁇ can exhibit 100% coverage within five to six months of planting as compared to ⁇ El Toro ⁇ , which takes nine to ten months under similar conditions.
  • ⁇ SS-500 ⁇ has been measured at 30 inches in length after a three-week growth period, as shown in FIG. 3.
  • the growth and morphology of ⁇ SS-500 ⁇ is further characterized in Table I, which presents data regarding blade width, blade length, internode length, stolon width, and spike length for ⁇ SS-500 ⁇ in comparison with other varieties of Zoysia.
  • ⁇ SS-500 ⁇ unlike many Zoysia grasses, can be easily mowed with a rotary mower due to its large blade size and its open growth habitat. This trait is important as it reduces general maintenance and allows the use of less expensive mowing equipment.
  • ⁇ SS-500 ⁇ spreads by stolons and rhizomes and forms an open uniform groundcover.
  • the stolons are grey-purple in color (183B).
  • Stolons are about 4 to 5 millimeters in width with nodes about 3 to 3.5 centimeters apart.
  • the nodes of ⁇ SS-500 ⁇ root adventitiously.
  • New leaves are rolled into bud shoots.
  • Mature leaves are about 5 to 5.5 millimeters in width and can reach 14 centimeters in length.
  • Mature leaves have a dark green color on both upper and lower surfaces corresponding to 137A.
  • the leaves have hairs along the edges, which are more concentrated along the end of the leaf.
  • the leaves taper to a point.
  • the culm has the same dark green color (137A) as the leaf blade.
  • the collar is large and continuous.
  • the inflorescence is a single spike at the top of the stem.
  • the anther is white (155D) and the stigmas are green-white (157C) in color.
  • the seed pods are greyed purple at the top portion (i.e., tip) and fade to a green-white color at the bottom portion of the seed pod (183C and 157C, respectively).
  • the root structure is massive and deep, with large individual roots.
  • ⁇ SS-500 ⁇ demonstrates an extended season as compared with other Zoysia grasses, The ⁇ SS-500 ⁇ variety exhibits early greening in the Spring and late Fall color retention.
  • ⁇ SS-500 ⁇ was removed from the production field and transplanted into plug trays for further trials and testing. After several series of cuttings and transplantings were made, ⁇ SS-500 ⁇ retained the color, size, and node length characteristics that were originally noted. ⁇ SS-500 ⁇ was taken transplanted to field plots for examination.
  • Roots of ⁇ SS-500 ⁇ are generally larger in diameter than those of ⁇ Wild Zoysia ⁇ .
  • Sprigs of ⁇ SS-500 ⁇ establish roots quickly, within three to six days, and the root system is massive and deep. Other advantages both physically and morphologically are apparent from the figures provided herein.
  • ⁇ SS-500 ⁇ unlike many Zoysia grasses, can be easily mowed with a rotary mower due to its large blade size and its open growth habit. This trait reduces general maintenance and allow the use of less expensive mowing equipment.
  • ⁇ SS-500 ⁇ can be propagated from sod, plugs, sprigs, stolons, tillers or rhizome pieces. Because ⁇ SS-500 ⁇ regrows from rhizomes, ribbons or strips of grass are not required to be left in the field for successful re-growth after sod harvesting. Seed stability is undetermined at this time, but indications suggest that there is little to no viability.
  • ⁇ SS-500 ⁇ is similar to other Zoysia japonica varieties in that it requires full sun to slight shade. Its hardiness zone is unknown; cold hardiness studies are currently underway. ⁇ SS-500 ⁇ has been noted to turn brown after heavy frost, but has excellent fall color retention.
  • ⁇ SS-500 ⁇ has good drought tolerance, most likely due to the massive root system.
  • ⁇ SS-500 ⁇ has resistance to leaf spot disease and only limited susceptibility to armyworms as compared with other grasses.
  • DNA fingerprint analysis was carried out on ⁇ SS-500 ⁇ at the University of Tennessee using the methods described in U.S. Pat. No. 5,413,909 and by Caetano-Anolles et al., (1991) Bio/Technology 9:553. Cultivar ⁇ SS-300 ⁇ and standards ⁇ Meyer ⁇ , ⁇ El Toro ⁇ , and ⁇ Emerald ⁇ (unpatented) were also analyzed. Genomic DNA was isolated from freshly grown material. No evidence of diseased tissue or weeds was detected. Several young blades of grass were ground in liquid nitrogen and DNA extracted. DNA was extracted in duplicate from the supplied samples.
  • the DNA was quantified by fluorimetry, then was diluted and amplified using the standard high primer-low template DNA ratio using DNA amplification fingerprinting (DAF) as described in U.S. Pat. No. 5,413,909 and Caetano-Anolles et al., (1991) Bio/Technology 9:553.
  • DAF DNA amplification fingerprinting
  • Primers 8.6i and 10.6e were used.
  • Amplification occurred in an MJR PT200 thermocycler, using the optimized DAF amplification program DAF15. This program involves fast ramping, high annealing temperature (55° C.), and an extension step at 72° C.
  • the DNA was extracted in duplicate from the samples.
  • the DNA was diluted and amplified as described above. Duplicate amplification products were separated by polyacrylamide gel electrophoresis.
  • FIG. 12 and FIG. 13 Two gels showing the result of DNA analysis are shown in FIG. 12 and FIG. 13, using primer 8.6i or 10.6e, respectively. About 15 major amplification products, seen as strong bands, and about 30 minor ones are commonly seen. The lanes on the sides represent molecular size markers (1000, 700, 500 (doublet), 400, 300, 200 and 100 bp) used to determine the size of the amplification products.
  • molecular size markers 1000, 700, 500 (doublet), 400, 300, 200 and 100 bp
  • primer 8.6i (FIG. 12)
  • several polymorphic bands were seen that distinguish ⁇ SS-500 ⁇ from the other Zoysia cultivars.
  • the primer 8.6i clearly distinguishes the ⁇ Meyer ⁇ , ⁇ El Toro ⁇ and ⁇ Emerald ⁇ standards, e.g., in the 400-500 bp region.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physiology (AREA)
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  • Natural Medicines & Medicinal Plants (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

A new and distinct variety of Zoysia japonica turfgrass, called `SS-500`, is characterized by its color, long and wide leaf blades, large stolons, an open growth habit, and rapid establishment rate.

Description

BACKGROUND OF THE INVENTION
The present invention relates to a new and distinct variety of a perennial Zoysia grass, discovered on a Brazilian turf farm in Sao Paolo during a routine field inspection. The grass was found in a production field of a common Brazilian grass known as `Wild Zoysia` (unpatented). The new grass is believed to be a sport of `Wild Zoysia` due to its color and its larger leaf size and longer node length compared to `Wild Zoysia`. The novel grass, termed `SS-500`, was propagated asexually in Sao Paolo, Brazil. `SS-500` is a distinct, asexually propagated variety of Zoysia grass. `SS-500` is the varietal designation of this new grass. The name `SS-500` may also designate this plant in commerce.
SUMMARY OF THE INVENTION
`SS-500` is a attractive green Zoysia grass, with an open growth habit. It is characterized by the combination of its color, open growth habit, and large leaf blade length and width.
BRIEF DESCRIPTION OF THE FIGURES
FIG. 1 compares stolons of `SS-500` and `SS-300`. `SS-300` is another new variety of Zoysia grass, and is the subject of co-pending U.S. Plant Patent application Ser. No. 09/028,031. A metric ruler is provided for scale.
FIG. 2 compares stolons of `SS-500` and Zoysia cultivars `Meyer`, `El Toro`, and `SS-300`, next to a metric scale.
FIG. 3 shows a runner of `SS-500` that grew to over 30 inches in less than a month.
FIG. 4 shows a plug of `SS-500`.
FIG. 5 shows `SS-500` in the field at three months. Compare this to FIG. 6.
FIG. 6 shows `El Toro` in the field at three months, grown under the same conditions as the `SS-500` depicted in FIG. 5.
FIG. 7 shows the seedhead of `SS-500`.
FIG. 8 shows one view of the root structure of `SS-500`.
FIG. 9 shows one view of the root structure of `SS-500`.
FIG. 10 provides a comparison of the `SS-500` (top) and `SS-300` (bottom) cultivars.
FIG. 11 shows a close-up of stem color of `SS-500`.
FIG. 12 is a gel showing DNA fingerprint analysis of `SS-500` in comparison to other varieties of zoyziagrass using primer 8.6i. M=molecular size markers in base pairs. Areas of distinct DNA amplification differences are indicated by small arrows.
FIG. 13 is a gel showing DNA fingerprint analysis of `SS-500` in comparison to other varieties of zoyziagrass using primer 10.6e. M=molecular size markers in base pairs. Areas of distinct DNA amplification differences are indicated by small arrows.
DETAILED DESCRIPTION OF THE VARIETY
The following is a detailed description of the new Zoysia grass variety known as `SS-500`, based upon observations of the plant grown in plug trays and field plots. Color notations are based on The Royal Horticultural Society Colour Chart, The Royal Horticultural Society, London.
`SS-500` is a perennial, vegetatively propagated Zoysia grass, believed to be a variety of Zoysia japonica. The parent of `SS-500` is believed to be a native Brazilian variety of Zoysia japonica, commonly known as `Wild Zoysia`. `SS-500` is a wide-bladed grass with an open growth habit. Applicants have asexually propagated `SS-500` by means of stolons and rhizomes. Applicants have discovered the novelty and distinctness of `SS-500` compared to other varieties of Zoysia.
`SS-500` has an open growth habit, large stolons and leaf blades, and a massive root system. Side-by-side comparisons of `SS-500` to variety `El Toro` (a standard for rapid growth of Zoysia) demonstrated that `SS-500` exhibits rapid growth; complete coverage can occur within five to six months after planting, compared to `El Toro` which takes nine to ten months under similar conditions. Each variety was planted in 8'×8' plots using 4-inch prerooted grass plugs planted 12-inches on center to determine coverage rates.
`SS-500` is characterized by its large leaf blade width and length, open growth habit and large stolon size, and other characteristics described herein. `SS-500` has a rapid establishment rate, high tolerance to drought, good fall color retention rates, and good tolerance to herbicides MSMA and 2-4-D.
DIMENSIONS AND GROWTH HABIT
`SS-500` was discovered on a Brazilian turf farm in a production field of a common Brazilian grass known as `Wild Zoysia`. The new grass was initially believed to be an `off-type`, or sport, of `Wild Zoysia` due to its color and its larger leaf size (compared to `Wild Zoysia`). `SS-500` exhibits an open growth habit and large leaf width and length, compared to other Zoysia grasses. `SS-500` is low growing during the initial stage of coverage. After the ground is covered the grass becomes upright and open in habit. The grass spreads by stolons and rhizomes that form an open uniform groundcover. The root structure of `SS-500` is massive and deep, with large individual roots.
The leaf blade width and length of `SS-500` are larger than `El Toro` or other comparable types of Zoysia. `SS-500` has a more open growth habit, fewer seedheads, and longer as well as wider leaf blades than `Wild Zoysia`, `El Toro` Zoysia (U.S. Plant Pat. No. 5,845), or `Meyer` (unpatented) Zoysia. Further comparison testing to variety `El Toro`, a standard for rapid growth of Zoysia, demonstrates that `SS-500` can exhibit 100% coverage within five to six months of planting as compared to `El Toro`, which takes nine to ten months under similar conditions. This characteristic may be beneficial to the turf industry, as providing a grass suitable for erosion control. Runners of `SS-500` have been measured at 30 inches in length after a three-week growth period, as shown in FIG. 3. The growth and morphology of `SS-500` is further characterized in Table I, which presents data regarding blade width, blade length, internode length, stolon width, and spike length for `SS-500` in comparison with other varieties of Zoysia.
              TABLE I                                                     
______________________________________                                    
Length and Width Data                                                     
Blade        Blade    Internode                                           
                               Stolon Spike                               
Width                                           Length                    
(mm)                                            (mm)                      
______________________________________                                    
`SS-300`                                                                  
        1.9-2.3  30-40    18-25  1.0-1.5                                  
                                        16-18                             
`SS-500`                                                                  
                                                  30-40                   
`El Torro`                                                                
                                                  27-30                   
`Meyer`                                           24-28                   
`Emerald`*                                                                
                                                  12-14                   
______________________________________                                    
 *unpatented                                                              
 Reference: Test plots located at Elsberry Greenhouse in Ruskin, FL.      
 Reference: Test plots located at Bethel Farms in Arcadia, FL.            
 8' × 8' plots                                                      
 Plugged by Elsberry Greenhouse in Ruskin, FL on 2/03/98.                 
 Plugged by Bethel Farms in Arcadia, FL on 2/18/99.                       
 Material attained by special permit USDA Quarantine Lab, Beltsville, MD.
`SS-500`, unlike many Zoysia grasses, can be easily mowed with a rotary mower due to its large blade size and its open growth habitat. This trait is important as it reduces general maintenance and allows the use of less expensive mowing equipment.
`SS-500` spreads by stolons and rhizomes and forms an open uniform groundcover. The stolons are grey-purple in color (183B). Stolons are about 4 to 5 millimeters in width with nodes about 3 to 3.5 centimeters apart. The nodes of `SS-500` root adventitiously. New leaves are rolled into bud shoots. Mature leaves are about 5 to 5.5 millimeters in width and can reach 14 centimeters in length. Mature leaves have a dark green color on both upper and lower surfaces corresponding to 137A. The leaves have hairs along the edges, which are more concentrated along the end of the leaf. The leaves taper to a point. The culm has the same dark green color (137A) as the leaf blade. The collar is large and continuous. The inflorescence is a single spike at the top of the stem. The anther is white (155D) and the stigmas are green-white (157C) in color. The seed pods are greyed purple at the top portion (i.e., tip) and fade to a green-white color at the bottom portion of the seed pod (183C and 157C, respectively). The root structure is massive and deep, with large individual roots.
`SS-500` demonstrates an extended season as compared with other Zoysia grasses, The `SS-500` variety exhibits early greening in the Spring and late Fall color retention.
These and other features and characteristics of the `SS-500` cultivar are apparent from the figures provided herein.
REPRODUCTION
After its initial discovery, `SS-500` was removed from the production field and transplanted into plug trays for further trials and testing. After several series of cuttings and transplantings were made, `SS-500` retained the color, size, and node length characteristics that were originally noted. `SS-500` was taken transplanted to field plots for examination.
During examination of `SS-500` transplanted to field plots, it was noted that `SS-500` spread at a rate faster than `Wild Zoysia` (30-40% faster). Further testing and evaluation determined that the root system of `SS-500` is more aggressive than that of `Wild Zoysia`, and equally dense to that of `Wild Zoysia`. Roots of `SS-500` are generally larger in diameter than those of `Wild Zoysia`. Further comparison testing to variety `El Toro`, a standard for rapid growth of Zoysia, demonstrates that `SS-500` can exhibit 100% coverage within five to six months of planting, compared to `El Toro` which takes nine to ten months under similar conditions. More extensive data regarding coverage rates of `SS-500` in comparison with other Zoysias are presented in Table 2. Other turf characteristics are provided in Table 3 and Table 4.
              TABLE 2                                                     
______________________________________                                    
Percent Coverage Data (%)                                                 
             30      60      90    120   150                              
Plant #                                             Days                  
______________________________________                                    
`SS-300`                                                                  
        20       30      40    55    80    95                             
`SS-500`                                                                  
                 35 20   45    60    85    100                            
`El Torro`                                                                
          20     25      35    50    65    80                             
`Meyer`          250     30    45    55    70                             
`Emerald`*                                                                
                 20    20                                                 
                         25    35    45    55                             
______________________________________                                    
 *unpatented                                                              
 Reference: Test plots located at Elsberry Greenhouse in Ruskin, FL.      
 Reference: Test plots located at Bethel Farms in Arcadia, FL.            
 8' × 8' plots                                                      
 Plugged by Elsberry Greenhouse in Ruskin, FL on 2/03/98.                 
 Plugged by Bethel Farms in Arcadia, FL on 2/18/99.                       
 Material attained by special permit USDA Quarantine Lab, Beltsville, MD. 

              TABLE 3                                                     
______________________________________                                    
Zoysia Variety Evaluation                                                 
Turf         Seedhead Turf     Turf   Disease                             
Density      Presence Color    Quality                                    
                                      Presence                            
______________________________________                                    
`El Toro`                                                                 
        78.8     17.5     6.25   7.63   22.5                              
`SS-500`                                                                  
        92.5     18.8     6.25 8.00                                       
                                 15.0                                     
`Meyer` 36.3     0.0      8.13   6.25   58.5                              
`SS-300`                                                                  
        93.8     0.0      6.38   7.38   53.8                              
`Emerald`                                                                 
        17.5     0.0      7.38   6.63   58.8                              
Rating  0-100%   0-100%   1-9    1-9    1-100%                            
Scale                     1 =  light                                      
                                 1 = poor                                 
                          9 = dark                                        
                                 9 = good                                 
Rating  7/24/99  7/24/99  7/24/99                                         
                                 7/24/99                                  
                                        7/24/99                           
Date                                                                      
______________________________________                                    
 Evaluations performed at Sod Solutions Coastal Research Center,          
 Charleston, South Carolina.                                              

              TABLE 4                                                     
______________________________________                                    
Zoysia Variety Evaluation                                                 
        Turf      Stolon     Plug    Seedhead                             
        Quality   Regrowth   Mortality                                    
                                     Presence                             
______________________________________                                    
`El Toro`                                                                 
         7.88     29.3       0.0     30.0                                 
`SS-500`                                                                  
                 8.00                                                     
                  47.0       0.0     85.0                                 
`Meyer`          8.38                                                     
                  4.3        0.0     11.3                                 
`SS-300`                                                                  
        8.63      150.0      0.0     1.3                                  
`Emerald`                                                                 
                7.00                                                      
                  26.5       0.3     10.0                                 
Rating  1-9       Number     Percent 1-100%                               
Scale            1 = poor                                                 
                 9 = good                                                 
Rating  5/7/99    5/7/99     5/7/99             5/7/99                    
Date                                                                      
______________________________________                                    
        Internode Blade      Turf    Plug                                 
        Length    Width      Density Width                                
______________________________________                                    
`El Toro`                                                                 
              26.0                                                        
                  3.20       7.5     15.47                                
`SS-500`                                                                  
        35.0      5.23       10.0    15.90                                
`Meyer`          34.0                                                     
                  3.10       0.0     12.60                                
`SS-300`                                                                  
                  2.280      11.3    15.50                                
`Emerald`                                                                 
        12.0      1.20       1.3     14.22                                
Rating  mm        mm         1-100%  cm                                   
Scale                                                                     
Rating          5/22/99                                                   
                  5/22/99    5/22/99 5/22/99                              
Date                                                                      
______________________________________                                    
 Evaluations performed at Sod Solutions Coastal Research Center,          
 Charleston, South Carolina.
Sprigs of `SS-500` establish roots quickly, within three to six days, and the root system is massive and deep. Other advantages both physically and morphologically are apparent from the figures provided herein.
`SS-500`, unlike many Zoysia grasses, can be easily mowed with a rotary mower due to its large blade size and its open growth habit. This trait reduces general maintenance and allow the use of less expensive mowing equipment.
`SS-500` can be propagated from sod, plugs, sprigs, stolons, tillers or rhizome pieces. Because `SS-500` regrows from rhizomes, ribbons or strips of grass are not required to be left in the field for successful re-growth after sod harvesting. Seed stability is undetermined at this time, but indications suggest that there is little to no viability.
Asexual propagation of `SS-500` was carried out in the state of Sao Paolo, Brazil. Asexual reproduction of `SS-500` by rhizomes and tillers has established that the characteristics and combination of characteristics noted in `SS-500` are transmitted during succeeding propagations.
ENVIRONMENTAL TOLERANCES
`SS-500` is similar to other Zoysia japonica varieties in that it requires full sun to slight shade. Its hardiness zone is unknown; cold hardiness studies are currently underway. `SS-500` has been noted to turn brown after heavy frost, but has excellent fall color retention.
`SS-500` has good drought tolerance, most likely due to the massive root system.
DISEASE RESISTANCE AND SUSCEPTIBILITY
`SS-500` has resistance to leaf spot disease and only limited susceptibility to armyworms as compared with other grasses.
DNA FINGERPRINT ANALYSIS
DNA fingerprint analysis was carried out on `SS-500` at the University of Tennessee using the methods described in U.S. Pat. No. 5,413,909 and by Caetano-Anolles et al., (1991) Bio/Technology 9:553. Cultivar `SS-300` and standards `Meyer`, `El Toro`, and `Emerald` (unpatented) were also analyzed. Genomic DNA was isolated from freshly grown material. No evidence of diseased tissue or weeds was detected. Several young blades of grass were ground in liquid nitrogen and DNA extracted. DNA was extracted in duplicate from the supplied samples. The DNA was quantified by fluorimetry, then was diluted and amplified using the standard high primer-low template DNA ratio using DNA amplification fingerprinting (DAF) as described in U.S. Pat. No. 5,413,909 and Caetano-Anolles et al., (1991) Bio/Technology 9:553. Stoffel fragment of Taq™ DNA polymerase (Perkin Elmer Corp.) was used to amplify genomic DNA. Primers 8.6i and 10.6e were used. Amplification occurred in an MJR PT200 thermocycler, using the optimized DAF amplification program DAF15. This program involves fast ramping, high annealing temperature (55° C.), and an extension step at 72° C. Resulting amplification products were resolved using SUPERGEL™ polyacrylamide gel electrophoresis (Bassam and Bentley, (1995) Biotechniques 19:568) and visualized by silver staining as described in U.S. Pat. No. 5,643,479 and Bassam et al., (1991) Anal. Biochemistry 196:80.
The DNA was extracted in duplicate from the samples. The DNA was diluted and amplified as described above. Duplicate amplification products were separated by polyacrylamide gel electrophoresis.
Two gels showing the result of DNA analysis are shown in FIG. 12 and FIG. 13, using primer 8.6i or 10.6e, respectively. About 15 major amplification products, seen as strong bands, and about 30 minor ones are commonly seen. The lanes on the sides represent molecular size markers (1000, 700, 500 (doublet), 400, 300, 200 and 100 bp) used to determine the size of the amplification products.
Using primer 8.6i (FIG. 12), several polymorphic bands were seen that distinguish `SS-500` from the other Zoysia cultivars. The primer 8.6i clearly distinguishes the `Meyer`, `El Toro` and `Emerald` standards, e.g., in the 400-500 bp region.
The `SS-300` and `SS-500` samples are similar to each other, but clearly different. The differences between these two cultivars are even more pronounced with the 10.6e primer (FIG. 13).
The results of the fingerprinting analysis indicated that both `SS-500` and `SS-300` are most closely related to `El Toro`. The `SS-500` and `SS-300` varieties appear to be related, but are clearly distinct from each other. Primers 8.6i and 10.6e showed clear polymorphisms for several major bands. Areas of distinct DNA amplification differences are indicated by small arrows in FIG. 12 and FIG. 13.

Claims (1)

That which is claimed is:
1. A new and distinct variety of Zoysia japonica grass plant named `SS-500`, substantially as described and illustrated, which has an open growth habit, rapid coverage rate, and large leaf blades.
US09/031,492 1998-02-26 1998-02-26 Zoysia grass plant named `SS-500` Expired - Lifetime USPP11466P (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USPP34352P3 (en) 2020-01-27 2022-06-14 Florida Foundation Seed Producers, Inc Zoysiagrass plant named ‘FAES1307’
USPP36415P2 (en) 2024-01-11 2025-01-28 Florida Foundation Seed Producers, Inc. Zoysiagrass plant named ‘FAES1319’

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USPP34352P3 (en) 2020-01-27 2022-06-14 Florida Foundation Seed Producers, Inc Zoysiagrass plant named ‘FAES1307’
USPP36415P2 (en) 2024-01-11 2025-01-28 Florida Foundation Seed Producers, Inc. Zoysiagrass plant named ‘FAES1319’

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