US9532523B2 - Edible bean line <11258> (vibrant) - Google Patents

Edible bean line <11258> (vibrant) Download PDF

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US9532523B2
US9532523B2 US14/252,926 US201414252926A US9532523B2 US 9532523 B2 US9532523 B2 US 9532523B2 US 201414252926 A US201414252926 A US 201414252926A US 9532523 B2 US9532523 B2 US 9532523B2
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edible bean
seed
plant
edible
vibrant
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Ron Shellenberger
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ADM Edible Bean Specialties Inc
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ADM Edible Bean Specialties Inc
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10Seeds
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/54Leguminosae or Fabaceae, e.g. soybean, alfalfa or peanut
    • A01H6/545Phaseolus, e.g. kidney beans, scarlet runners or spotted beans
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04Plant cells or tissues

Definitions

  • the present invention relates generally to the field of plant breeding, and more specifically to edible bean line ⁇ 11258> (Vibrant).
  • Legumes are packed with fiber and protein, as well as being a good source of numerous vitamins and minerals. Legumes also have a low glycemic index and may help play a role in maintaining normal blood sugar levels; increasing digestive health; and even possibly improving heart health. Yet, legumes are an underutilized food in the United States diet.
  • pinto bean One type of edible bean that is sold and consumed is the pinto bean.
  • the seed coat of pinto beans may darken which may be viewed by consumers as a product flaw.
  • the present invention helps fulfill these needs and discloses a pinto bean variety for use in breeding new bean varieties, as well as a foodstuff.
  • an edible bean seed designated as Vibrant a sample of the edible bean seed deposited under accession no. PI 673082, is disclosed.
  • methods of using the edible bean seed Vibrant for breeding new varieties of bean seed are disclosed. Seeds of the edible bean seed designated as Vibrant are also disclosed.
  • FIG. 1 shows the breeding history of edible bean line ⁇ 11258> (Vibrant) and its parents.
  • an edible bean line designated as ⁇ 11258>, or (Vibrant) is disclosed.
  • Vibrant will be used to refer to edible bean variety ⁇ 11258>.
  • bean plants having the physiological and morphological characteristics of the edible bean line designated as Vibrant are further disclosed.
  • seed of the edible bean line designated as Vibrant is disclosed.
  • Such seed may be an essentially homogenous population of the edible bean line designated as Vibrant, wherein such seed of the edible bean line designated as Vibrant is essentially free of other seed.
  • the seed of the present invention comprises at least 95% or more of seed of the edible bean line designated as Vibrant.
  • tissue culture of regenerable cells of the edible bean line designated as Vibrant are disclosed.
  • tissue culture may be capable of expressing all of the physiological and morphological characteristics of the edible bean line designated as Vibrant, and be capable of regenerating plants having the same genotype of plants of the edible bean line designated as Vibrant.
  • the tissue culture may be obtained from embryos, meristems, cotyledons, pollen, leaves, anthers, roots, root tips, pistil, flower, seed and/or stalks.
  • breeding methods for producing edible bean seeds, plants and/or pods using the edible bean line designated as Vibrant of the present invention as a parent are disclosed. Such breeding methods may be used to prepare hybrid edible bean seed or plants or inbred edible bean seed or plants, where seed ultimately produced using the edible bean line designated as Vibrant as at least one of the parents are included within the scope of this invention.
  • methods of crossing the edible bean line designated as Vibrant with itself of a second plant and the seeds and plants produced by such methods are disclosed. Such methods may be used for propagating edible bean line designated as Vibrant, or can be used to produce hybrid dry bean seeds and the plants grown therefrom.
  • the present invention discloses a process for producing beans comprising obtaining a plant of the edible bean line designated as Vibrant, cultivating the plant to maturity, and collecting and/or harvesting beans from the mature plant.
  • Such beans may be used ultimately as a foodstuff, as seed or as parent in a breeding program.
  • the present invention further discloses methods and compositions relating to plants, seeds and derivatives of edible bean line designated as Vibrant.
  • This line has an erect growth habit, is indeterminate, has guides that are medium to long, and has no ability to climb.
  • Vibrant is a pinto bean variety, exhibiting traits including, but not limited to, early season maturity and slow darkening. Observations and seed increase show that edible bean line designated as Vibrant shows uniformity and stability, without variants.
  • the edible bean line designated as Vibrant exhibits improved traits including, without limitation, resistance to darkening of the seed coat conferred by presence of the recessive sd gene.
  • the development of the edible bean line designated as Vibrant can be summarized as follows. A cross was made between ProVita's slow darkening pinto breeding line ‘SDP 1533’ as the female parent and the Ameriseed variety ‘Sinaloa’ as the male parent. The cross was made during the summer in Year 1 in the field. The edible bean line designated as Vibrant was derived from this cross.
  • the breeding history of edible bean line designated as Vibrant is shown in FIG. 1 .
  • Table 2 depicts the physiological and morphological characteristics of edible bean line designated as Vibrant.
  • Vibrant possess a faint red stripe on a maturing pod, which may not be visible when the pod is dry.
  • the edible bean line designated as Vibrant of the present invention carries a recessive sd gene that confers resistance to darkening due to age or environmental conditions. Such slow darkening is of particular benefit to pinto bean crops grown in environments that darken traditional pinto beans. Vibrant will retain improved bright white color through adverse weather conditions or upon storage, whereas the parent of Vibrant, Sinaloa, will darken significantly under such conditions.
  • Vibrant has faint red striping on the mature pods, whereas Sinaloa expresses more prominent red striping on the mature pods.
  • Sinaloa also has expressed pigment in the stems, petioles, and peduncles, while Vibrant does not have pigment in the stems, petioles, or peduncles.
  • a deposit of the edible bean line designated as Vibrant of the present invention will be made with the National Center for Genetic Resources Preservation, Fort Collins, Colo. The date of deposit was Oct. 3, 2014. Upon issuance of a patent, all restrictions upon the deposit will be removed, and the deposit is intended to meet all of the requirements of 37 C.F.R. ⁇ 1.801-1.809.
  • the accession number for the deposited seeds of edible bean line designated as Vibrant is PI 673082. The deposit will be maintained in the depository for a period of 30 years, or 5 years after the last request, or for the enforceable life of the patent, whichever is longer, and will be replaced if necessary during such period.

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  • Developmental Biology & Embryology (AREA)
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Abstract

An edible bean seed designated as <11258> or Vibrant, a sample of the edible bean deposited under accession no. PI 673082 is disclosed. Methods of using the edible bean seed designated as <11258> or Vibrant for breeding new varieties of bean seed are also disclosed, as well as seeds of the edible bean seed designated as <11258> or Vibrant.

Description

TECHNICAL FIELD
The present invention relates generally to the field of plant breeding, and more specifically to edible bean line <11258> (Vibrant).
BACKGROUND OF THE INVENTION
In the United States, there is increasing concern over the consumption of high sugar and high fat foods by the population. An alarming increase in obesity among the citizens in the United States exists since many people in the United States are overweight due to a lack of exercise and poor eating habits.
Beans and peas are listed under both the Vegetables, and the Meats and Beans categories of the Dietary Guidelines Pyramid, thus, emphasizing the healthy nature of legumes in the diet. In addition to the emphasis on lower fat and lower sugar diets, beneficial effects of legumes are becoming apparent. Legumes are packed with fiber and protein, as well as being a good source of numerous vitamins and minerals. Legumes also have a low glycemic index and may help play a role in maintaining normal blood sugar levels; increasing digestive health; and even possibly improving heart health. Yet, legumes are an underutilized food in the United States diet.
One type of edible bean that is sold and consumed is the pinto bean. During storage, the seed coat of pinto beans may darken which may be viewed by consumers as a product flaw. Thus, needs exist for varieties of pinto beans that either do not darken or take longer to darken such that they are consumed before the seed coat darkens.
SUMMARY OF THE INVENTION
In each of its various embodiments, the present invention helps fulfill these needs and discloses a pinto bean variety for use in breeding new bean varieties, as well as a foodstuff.
In one embodiment, an edible bean seed designated as Vibrant, a sample of the edible bean seed deposited under accession no. PI 673082, is disclosed.
In another embodiment, methods of using the edible bean seed Vibrant for breeding new varieties of bean seed are disclosed. Seeds of the edible bean seed designated as Vibrant are also disclosed.
BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 shows the breeding history of edible bean line <11258> (Vibrant) and its parents.
DETAILED DESCRIPTION OF THE INVENTION
In one embodiment, an edible bean line designated as <11258>, or (Vibrant), is disclosed. As used herein, the term Vibrant will be used to refer to edible bean variety <11258>. Also disclosed are bean plants having the physiological and morphological characteristics of the edible bean line designated as Vibrant. Parts of the bean plant including, without pollen, ovules, pods and cells, as well as uses of such parts in breeding are further disclosed.
In another embodiment, seed of the edible bean line designated as Vibrant is disclosed. Such seed may be an essentially homogenous population of the edible bean line designated as Vibrant, wherein such seed of the edible bean line designated as Vibrant is essentially free of other seed. Accordingly, the seed of the present invention comprises at least 95% or more of seed of the edible bean line designated as Vibrant.
In yet a further embodiment, tissue culture of regenerable cells of the edible bean line designated as Vibrant are disclosed. Such tissue culture may be capable of expressing all of the physiological and morphological characteristics of the edible bean line designated as Vibrant, and be capable of regenerating plants having the same genotype of plants of the edible bean line designated as Vibrant. The tissue culture may be obtained from embryos, meristems, cotyledons, pollen, leaves, anthers, roots, root tips, pistil, flower, seed and/or stalks.
In an additional embodiment, breeding methods for producing edible bean seeds, plants and/or pods using the edible bean line designated as Vibrant of the present invention as a parent are disclosed. Such breeding methods may be used to prepare hybrid edible bean seed or plants or inbred edible bean seed or plants, where seed ultimately produced using the edible bean line designated as Vibrant as at least one of the parents are included within the scope of this invention.
In one embodiment, methods of crossing the edible bean line designated as Vibrant with itself of a second plant and the seeds and plants produced by such methods are disclosed. Such methods may be used for propagating edible bean line designated as Vibrant, or can be used to produce hybrid dry bean seeds and the plants grown therefrom.
In a further embodiment, the present invention discloses a process for producing beans comprising obtaining a plant of the edible bean line designated as Vibrant, cultivating the plant to maturity, and collecting and/or harvesting beans from the mature plant. Such beans may be used ultimately as a foodstuff, as seed or as parent in a breeding program.
The present invention further discloses methods and compositions relating to plants, seeds and derivatives of edible bean line designated as Vibrant. This line has an erect growth habit, is indeterminate, has guides that are medium to long, and has no ability to climb. Vibrant is a pinto bean variety, exhibiting traits including, but not limited to, early season maturity and slow darkening. Observations and seed increase show that edible bean line designated as Vibrant shows uniformity and stability, without variants.
Origin and Breeding History.
The edible bean line designated as Vibrant exhibits improved traits including, without limitation, resistance to darkening of the seed coat conferred by presence of the recessive sd gene. The development of the edible bean line designated as Vibrant can be summarized as follows. A cross was made between ProVita's slow darkening pinto breeding line ‘SDP 1533’ as the female parent and the Ameriseed variety ‘Sinaloa’ as the male parent. The cross was made during the summer in Year 1 in the field. The edible bean line designated as Vibrant was derived from this cross.
The breeding history of edible bean line designated as Vibrant is shown in FIG. 1.
TABLE 1
The following Table indicates the selections used to
produce the edible bean line designated as Vibrant.
Bulk Single Plant
Year Location Generation (lbs) Selections
Summer Twin Falls, ID-field F1 <2 lb
Year 1
Summer Twin Falls, ID-field F2 3 lbs
Year 2
Summer Twin Falls, ID-field F3 5 single plant
Year 3 selections
Summer Twin Falls, ID-field F4 2 single plant
Year 4 selections
Summer Twin Falls, ID-field F5 1.5 lbs 2 single plant
Year 5 selections
Spring Kuna, ID- F6
Year 6 greenhouse
Summer Twin Falls, ID-field F7 65.5 lbs
Year 6
Summer Twin Falls, ID-field F8 1800 lbs
Year 7
Summer Twin Falls, ID-field F9 Commercial seed
Year 8 production
Selections were done in the F3, F4 and F5 generations as follows. In the F3 generation, there was segregation for architecture, maturity, seed size, and the slow darkening trait. Single plant selections were made for upright architecture, high pod set, yield, early maturity, and the slow darkening trait. In the F4 generation, single plant selections were made for upright architecture, high pod set, yield, early maturity, and the slow darkening trait. In the F5 generation, single plant selections were made for upright architecture, high pod set, yield, early maturity, and the slow darkening trait. Such selections resulted in the new edible bean line designated as Vibrant, which was observed to be uniform, stable and substantially free of any variants within commercially acceptable limits since the F9 generation. It is known that a small percentage of off-types may occur for a characteristic of edible bean line designated as Vibrant during multiplication.
Physiological and Morphological Characteristics of Edible Bean Line Designated as Vibrant.
Table 2 depicts the physiological and morphological characteristics of edible bean line designated as Vibrant.
TABLE 2
Characteristic Edible bean line designated as Vibrant
Market Class Pinto
Maturity Early (80-90 days)
Days from planting to 90
harvest maturity
Plant Habit
Type Erect growth habit-indeterminate, guides
medium to long with no ability to climb
Average height of Not measured
mature plant
Pod position High (lower pods not touching soil surface)
Adaptability to machine Adapted
harvest
Lodging resistance Good
Leaflet morphology
Leaflet Smooth and semiglossy
Shape Ovate
Apex of leaflet Acuminate
Base of leaflet Obtuse
Flower color and days
to bloom
Color of standard White
Color of wings White
Color of keel White
Days to 50% bloom 53
Pod Morphology
Green color pattern Solid
Mature color pattern Striped
Green primary color Green
Mature primary color Yellow
Green color modifier Light medium
Mature color modifier Light
Green secondary color None
Mature secondary color Red, faint red stripe when maturing
Green cross section shape Pear
Mature cross section shape Pear
Green pod curvature Straight
Mature pod curvature Straight
Green pod beak orientation Curved downward
Mature pod beak Curved downward
orientation
Green constrictions Slight
Mature constrictions Slight
Average number of seeds/ 6.0
pod
Seed Color Semishiny and polychrome
Primary color White
Secondary color Brown
Color pattern Mottled
Hilar ring Present
Hilar ring color Yellow
Seed Shape and Weight
Shape of seed taken from Cuboid
middle of pod
Dry seed weight in g/100 g 36
Seeds (adjusted io 12%
moisture)
Anthocyanin Pigmentation*
Flowers Absent
Leaves Absent
Stems Absent
Petioles Absent
Pods Present, faint red stripe when maturing
Peduncles Absent
Seeds Present
Nodes Absent
Known Disease Reaction None
Vibrant possess a faint red stripe on a maturing pod, which may not be visible when the pod is dry.
Slow Darkening of Edible Bean Line Designated as Vibrant.
The edible bean line designated as Vibrant of the present invention carries a recessive sd gene that confers resistance to darkening due to age or environmental conditions. Such slow darkening is of particular benefit to pinto bean crops grown in environments that darken traditional pinto beans. Vibrant will retain improved bright white color through adverse weather conditions or upon storage, whereas the parent of Vibrant, Sinaloa, will darken significantly under such conditions.
Vibrant has faint red striping on the mature pods, whereas Sinaloa expresses more prominent red striping on the mature pods. Sinaloa also has expressed pigment in the stems, petioles, and peduncles, while Vibrant does not have pigment in the stems, petioles, or peduncles.
Deposit Information.
A deposit of the edible bean line designated as Vibrant of the present invention will be made with the National Center for Genetic Resources Preservation, Fort Collins, Colo. The date of deposit was Oct. 3, 2014. Upon issuance of a patent, all restrictions upon the deposit will be removed, and the deposit is intended to meet all of the requirements of 37 C.F.R. §1.801-1.809. The accession number for the deposited seeds of edible bean line designated as Vibrant is PI 673082. The deposit will be maintained in the depository for a period of 30 years, or 5 years after the last request, or for the enforceable life of the patent, whichever is longer, and will be replaced if necessary during such period.
The present invention has been described with reference to certain exemplary embodiments, legume products, compositions and uses thereof. However, it will be recognized by those of ordinary skill in the art that various substitutions, modifications or combinations of any of the exemplary embodiments may be made without departing from the spirit and scope of the invention. Thus, the invention is not limited by the description of the exemplary embodiment, but rather by the appended claims as originally filed.

Claims (15)

What is claimed is:
1. An edible bean seed designated as Vibrant, wherein a representative sample of the edible bean seed was deposited under the National Center for Genetic Resources Preservation accession no PI 673082.
2. A plant, or plant parts thereof, produced by growing the edible bean seed of claim 1.
3. Pollen of the plant of claim 2.
4. An edible bean plant, or plant parts thereof, having all of the physiological and morphological characteristics of the edible bean plant of claim 2.
5. A tissue culture of regenerable cells of the edible bean plant of claim 2.
6. The tissue culture of claim 5, wherein the cells are obtained from a plant part selected from the group consisting of leaf, pollen, embryo, meristematic cell, root, root tip, anther, stomatal cell, flower, seed, stem, pod and combinations of any thereof.
7. An edible bean plant regenerated from the tissue culture of claim 6, having all of the morphological and physiological characteristics of an edible bean plant grown from an edible bean seed designated as Vibrant, wherein a representative sample of the edible bean seed was deposited under the National Center for Genetic Resources Preservation accession no PI 673082.
8. A method for producing an edible bean seed comprising crossing two edible bean plants and harvesting the resulting edible bean seed, wherein at least one of the two edible bean plants is the edible bean plant of claim 2.
9. A method for producing a hybrid edible bean seed comprising crossing the edible bean plant of claim 2 with a second edible bean plant and harvesting the resulting hybrid edible bean seed.
10. A plurality of the edible bean seeds of claim 1.
11. A method of planting a field, comprising planting the plurality of the edible bean seeds of claim 10 in the field.
12. A process for producing edible beans for consumption, comprising processing the plurality of the edible bean seeds of claim 10 such that the plurality of the edible bean seeds are suitable for consumption.
13. A method for producing an edible bean seed designated as a Vibrant-derived edible bean seed, comprising:
crossing a plant of edible bean seed designated as Vibrant, wherein a representative sample of the seed was deposited under the National Center for Genetic Resources Preservation accession number PI 673082, with a second edible bean plant to yield progeny edible bean seed; and
growing the progeny edible bean seed to yield Vibrant-derived edible bean plants and allowing seed of Vibrant-derived edible beans plants to form.
14. The method of claim 13, wherein the second edible bean plant is transgenic.
15. An F1 edible bean designated as Vibrant-derived edible bean plant produced by the method of claim 13.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20220279746A1 (en) * 2021-03-05 2022-09-08 Adm Edible Bean Specialties, Inc. Edible bean line <17454> (mystic)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9775312B2 (en) * 2016-02-05 2017-10-03 Adm Edible Bean Specialties, Inc. Edible bean line <12324-1> (Radiant)

Citations (2)

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Publication number Priority date Publication date Assignee Title
US7700850B2 (en) * 2008-04-14 2010-04-20 Seminis Vegetable Seeds, Inc. Dry bean line 08560863
US8558077B2 (en) 2010-08-27 2013-10-15 Ameriseed Llc Edible bean line <06203> (sinaloa)

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7700850B2 (en) * 2008-04-14 2010-04-20 Seminis Vegetable Seeds, Inc. Dry bean line 08560863
US8558077B2 (en) 2010-08-27 2013-10-15 Ameriseed Llc Edible bean line <06203> (sinaloa)

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Felicetti et al., Simple Sequence Repeats Linked with Slow Darkening Trait in Pinto Bean Discovered by Single Nucleotide Polymorphism Assay and Whole Genome Sequencing, Crpo Science, vol. 52, Jul.-Aug. 2012, pp. 1600-1608, USA.
Plant Variety Protection Office, PVP Certificate 201100007, Plant Variety Protection Office, pp. 1-23, Aug. 18, 2011, USA.

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20220279746A1 (en) * 2021-03-05 2022-09-08 Adm Edible Bean Specialties, Inc. Edible bean line <17454> (mystic)

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