US9453046B2 - Activated carbon filtration for purification of benzodiazepine ADCs - Google Patents

Activated carbon filtration for purification of benzodiazepine ADCs Download PDF

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US9453046B2
US9453046B2 US14/774,348 US201414774348A US9453046B2 US 9453046 B2 US9453046 B2 US 9453046B2 US 201414774348 A US201414774348 A US 201414774348A US 9453046 B2 US9453046 B2 US 9453046B2
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benzodiazepine
drug
filtration
antibody
mixture
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US20160039870A1 (en
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Damon Meyer
Michael Sun
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Seagen Inc
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Seattle Genetics Inc
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/55—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having seven-membered rings, e.g. azelastine, pentylenetetrazole
    • A61K31/551—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having seven-membered rings, e.g. azelastine, pentylenetetrazole having two nitrogen atoms, e.g. dilazep
    • A61K31/5513—1,4-Benzodiazepines, e.g. diazepam or clozapine
    • A61K31/5517—1,4-Benzodiazepines, e.g. diazepam or clozapine condensed with five-membered rings having nitrogen as a ring hetero atom, e.g. imidazobenzodiazepines, triazolam
    • A61K47/48384—
    • A61K47/48561—
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
    • A61K47/6801—Drug-antibody or immunoglobulin conjugates defined by the pharmacologically or therapeutically active agent
    • A61K47/6803—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
    • A61K47/6835—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment the modifying agent being an antibody or an immunoglobulin bearing at least one antigen-binding site
    • A61K47/6849—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment the modifying agent being an antibody or an immunoglobulin bearing at least one antigen-binding site the antibody targeting a receptor, a cell surface antigen or a cell surface determinant
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
    • C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
    • C07D487/04—Ortho-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D519/00—Heterocyclic compounds containing more than one system of two or more relevant hetero rings condensed among themselves or condensed with a common carbocyclic ring system not provided for in groups C07D453/00 or C07D455/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/51—Complete heavy chain or Fd fragment, i.e. VH + CH1
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C07K2317/53—Hinge

Definitions

  • ADCs Antibody-drug conjugates
  • ADCs can provide an effective means of delivering a drug to a targeted site in a tissue or organism. Recognition of a target such as a tumor by the antibody minimizes exposure of non-target tissues to toxic chemotherapeutic agents and limits adverse effects associated with the toxicity of “free” drugs (i.e., unbound to a carrier such as an antibody).
  • ADCs can be prepared by a number of techniques. Prior to administration to a human or other subject, the conjugate is purified to remove free drugs and other impurities.
  • FIG. 1 provides a graph showing the concentration of quenched PBD drug-linker in the quenched conjugation reaction mixture following filtration through a Grade 5 3M Purification activated charcoal filter. The data demonstrates an approximately 60 fold reduction in quenched drug-linker level, with the maximum capacity of the filter not being reached. Filled diamonds represent the quenched drug-linker and the unfilled squares represent the ADC recovery.
  • FIG. 2 provides a graph showing the concentration of quenched PBD drug-linker in the QCR mixture following filtration through one (filled squares) or two (filled triangles) Grade 3 3M Purification activated charcoal filters. Passage through a second filter provides additional clearance. Open square indicates the ADC concentration following the first filtration and open triangles indicates the ADC concentration following the second filtration. A fraction of the ADC is lost with each filtration step.
  • FIG. 3 illustrates the ADC loss during recirculation through the carbon filter. Essentially all ADC loss occurred during initial contact of the QCR mixture with the activated charcoal filter. Data are reported as mg ADC removed/cm 2 filter surface area.
  • FIG. 4 provides a graph showing the clearance of quenched PBD drug-linker during re-circulation over an activated charcoal filter at different fluxes. The data indicate that for a given number of passages, lower flux provides better clearance.
  • FIG. 5 provides a graph showing the clearance of quenched PBD drug-linker from the quenched conjugation reaction mixture using different filters and protein loading concentrations.
  • FIG. 6 provides an exemplary purification scheme for the benzodiazepine ADCs
  • FIG. 7 provides an exemplary purification scheme for the benzodiazepine ADCs
  • the present invention is based, in part, on the discovery that many of the commonly employed methods for separating drug-related impurities from antibody-drug conjugate mixtures (e.g., tangential flow filtration) are unable to remove benzodiazepine drug-related impurities from mixtures comprising benzodiazepine ADCs and benzodiazepine drug-related impurities to an acceptable low level. It was surprisingly found that the benzodiazepine drug-related impurities could be effectively removed by filtration with activated charcoal. It was further discovered that re-circulation over an activated charcoal filter or multiple discrete filtrations using the same filter could further reduce the benzodiazepine drug-related impurities without having a negative impact on benzodiazepine ADC recovery.
  • Activated charcoal filtration can be performed by passing a mixture comprising benzodiazepine ADCs and benzodiazepine drug-related impurities through an activated charcoal filter.
  • the present invention provides a method for removing benzodiazepine drug-related impurities from mixtures comprising benzodiazepine ADCs and benzodiazepine drug-related impurities comprising the step of passing the mixture comprising through an activated charcoal filter (e.g., a filter in which activated charcoal is impregnated on a cellulose solid support or other type of solid support).
  • an activated charcoal filter e.g., a filter in which activated charcoal is impregnated on a cellulose solid support or other type of solid support.
  • Carbon filtration can also be performed, for example, by adding bulk activated charcoal, either as a powder, or as a suspension, to the mixture to be purified, mixing and or incubating the mixture, then removing the activated charcoal to which the impurity is adsorbed.
  • the present invention provides a method for removing benzodiazepine drug-related impurities from mixtures comprising benzodiazepine ADCs and benzodiazepine drug-related impurities comprising the step of contacting the mixture comprising benzodiazepine ADCs and benzodiazepine drug-related impurities with bulk activated carbon and passing the mixture through a filter.
  • the bulk activated carbon is in powder form.
  • the bulk activated charcoal is a suspension.
  • filtration is via multiple discrete filtrations through a single filter
  • 2 or more discrete filtrations can be performed.
  • 2 discrete filtrations can be performed, 3 discrete filtrations can be performed, or 3 or more discrete filtrations can be performed.
  • the recirculation can be continuous.
  • the mixture comprising the ADCs and impurities is recirculated from 3 to about 20 times through the filter.
  • the mixture can be recirculated through the filter.
  • the multiple passes through the filter can be multiple discrete passages through the filter.
  • the mixture can be recirculated through the filter.
  • the multiple passes through the filter can be multiple discrete passages through the filter.
  • the purified solution of benzodiazepine ADCs will have a concentration of benzodiazepine drug-related impurities of about 1 ⁇ M or less, preferably 0.2 ⁇ M, or even 0.1 ⁇ M or less. In preferred embodiments, the purified solution of benzodiazepine ADCs will have a concentration of quenched drug-linkers of about 1 ⁇ M or less, preferably 0.2 ⁇ M, or even 0.1 ⁇ M or less.
  • the benzodiazepine drug-related impurities that are removed from the mixture are quenched drug-linkers.
  • heterocycle refers to a monocyclic, bicyclic, or polycyclic ring system having from 3 to 14 ring atoms (also referred to as ring members) wherein at least one ring atom in at least one ring is a heteroatom selected from N, O, P, or S (and all combinations and subcombinations of ranges and specific numbers of carbon atoms and heteroatoms therein).
  • the heterocycle can have from 1 to 4 ring heteroatoms independently selected from N, O, P, or S.
  • One or more N, C, or S atoms in a heterocycle can be oxidized.
  • a monocyclic heterocycle preferably has 3 to 7 ring members (e.g., 2 to 6 carbon atoms and 1 to 3 heteroatoms independently selected from N, O, P, or S), and a bicyclic heterocycle preferably has 5 to 10 ring members (e.g., 4 to 9 carbon atoms and 1 to 3 heteroatoms independently selected from N, O, P, or S).
  • the ring that includes the heteroatom can be aromatic or non-aromatic.
  • Carbocycle refers to a saturated or unsaturated non-aromatic monocyclic, bicyclic, or polycyclic ring system having from 3 to 14 ring atoms (and all combinations and subcombinations of ranges and specific numbers of carbon atoms therein) wherein all of the ring atoms are carbon atoms.
  • Monocyclic carbocycles preferably have 3 to 6 ring atoms, still more preferably 5 or 6 ring atoms.
  • Carbocycles preferably have 3 to 8 carbon ring atoms.
  • phrases “pharmaceutically acceptable salt,” as used herein, refers to pharmaceutically acceptable organic or inorganic salts of a compound.
  • the compound can contain at least one amino group, and accordingly acid addition salts can be formed with the amino group.
  • Exemplary salts include, but are not limited to, sulfate, trifluoroacetate, citrate, acetate, oxalate, chloride, bromide, iodide, nitrate, bisulfate, phosphate, acid phosphate, isonicotinate, lactate, salicylate, acid citrate, tartrate, oleate, tannate, pantothenate, bitartrate, ascorbate, succinate, maleate, gentisinate, fumarate, gluconate, glucuronate, saccharate, formate, benzoate, glutamate, methanesulfonate, ethanesulfonate, benzenesulfonate, p-toluene
  • a pharmaceutically acceptable salt may involve the inclusion of another molecule such as an acetate ion, a succinate ion or other counterion.
  • the counterion may be any organic or inorganic moiety that stabilizes the charge on the parent compound.
  • a pharmaceutically acceptable salt may have more than one charged atom in its structure. Instances where multiple charged atoms are part of the pharmaceutically acceptable salt can have multiple counter ions. Hence, a pharmaceutically acceptable salt can have one or more charged atoms and/or one or more counterion.
  • antibody is used herein to denote immunoglobulin proteins produced by the body in response to the presence of an antigen and that bind to the antigen, as well as antigen-binding fragments and engineered variants thereof.
  • antibody includes, for example, intact monoclonal antibodies comprising full-length immunoglobulin heavy and light chains (e.g., antibodies produced using hybridoma technology) and antigen-binding antibody fragments, such as F(ab′) 2 and Fab fragments.
  • antibody is used expansively to include any protein that comprises an antigen-binding site of an antibody and is capable of specifically binding to its antigen.
  • variable engineered antibodies or “engineered antibody” means antibodies wherein the amino acid sequence has been varied from that of a native antibody. Because of the relevance of recombinant DNA techniques in the generation of antibodies, one need not be confined to the sequences of amino acids found in natural antibodies; antibodies can be redesigned to obtain desired characteristics. The possible variations are many and range from the changing of just one or a few amino acids to the complete redesign of, for example, the variable or constant region. Changes in the constant region will, in general, be made in order to improve or alter characteristics such as, e.g., complement fixation, interaction with cells, and other effector functions. Typically, changes in the variable region will be made in order to improve the antigen-binding characteristics, improve variable region stability, or reduce the risk of immunogenicity.
  • an “antigen-binding site of an antibody” is that portion of an antibody that is sufficient to bind to its antigen.
  • the minimum such region is typically a variable domain or a genetically engineered variant thereof.
  • Single-domain binding sites can be generated from camelid antibodies (see Muyldermans and Lauwereys, J. Mol. Recog. 12:131-140, 1999; Nguyen et al., EMBO J. 19:921-930, 2000) or from VH domains of other species to produce single-domain antibodies (“dAbs”; see Ward et al., Nature 341:544-546, 1989; U.S. Pat. No. 6,248,516 to Winter et al.).
  • an antigen-binding site is a polypeptide region having only 2 complementarity determining regions (CDRs) of a naturally or non-naturally (e.g., mutagenized) occurring heavy chain variable domain or light chain variable domain, or combination thereof (see, e.g., Pessi et al., Nature 362:367-369, 1993; Qiu et al., Nature Biotechnol. 25:921-929, 2007). More commonly, an antigen-binding site of an antibody comprises both a heavy chain variable (VH) domain and a light chain variable (VL) domain that bind to a common epitope.
  • VH heavy chain variable
  • VL light chain variable
  • an antibody may include one or more components in addition to an antigen-binding site, such as, for example, a second antigen-binding site of an antibody (which may bind to the same or a different epitope or to the same or a different antigen), a peptide linker, an immunoglobulin constant region, an immunoglobulin hinge, an amphipathic helix (see Pack and Pluckthun, Biochem.
  • a non-peptide linker an oligonucleotide (see Chaudri et al., FEBS Letters 450:23-26, 1999), a cytostatic or cytotoxic drug, and the like, and may be a monomeric or multimeric protein.
  • molecules comprising an antigen-binding site of an antibody include, for example, Fv, single-chain Fv (scFv), Fab, Fab′, F(ab′) 2 , F(ab)c, diabodies, dAbs, minibodies, nanobodies, Fab-scFv fusions, bispecific (scFv) 4 -IgG, and bispecific (scFv) 2 -Fab.
  • immunoglobulin refers to a protein consisting of one or more polypeptides substantially encoded by immunoglobulin gene(s).
  • One form of immunoglobulin constitutes the basic structural unit of native (i.e., natural) antibodies in vertebrates. This form is a tetramer and consists of two identical pairs of immunoglobulin chains, each pair having one light chain and one heavy chain. In each pair, the light and heavy chain variable regions (VL and VH) are together primarily responsible for binding to an antigen, and the constant regions are primarily responsible for the antibody effector functions.
  • Five classes of immunoglobulin protein (IgG, IgA, IgM, IgD, and IgE) have been identified in higher vertebrates.
  • IgG comprises the major class; it normally exists as the second most abundant protein found in plasma. In humans, IgG consists of four subclasses, designated IgG1, IgG2, IgG3, and IgG4.
  • the heavy chain constant regions of the IgG class are identified with the Greek symbol ⁇ .
  • immunoglobulins of the IgG1 subclass contain a ⁇ 1 heavy chain constant region. Each immunoglobulin heavy chain possesses a constant region that consists of constant region protein domains (CH1, hinge, CH2, and CH3; IgG3 also contains a CH4 domain) that are essentially invariant for a given subclass in a species.
  • DNA sequences encoding human and non-human immunoglobulin chains are known in the art.
  • immunoglobulin is used herein for its common meaning, denoting an intact antibody, its component chains, or fragments of chains, depending on the context.
  • Full-length immunoglobulin “light chains” (about 25 Kd or 214 amino acids) are encoded by a variable region gene at the amino-terminus (encoding about 110 amino acids) and a by a kappa or lambda constant region gene at the carboxyl-terminus.
  • Full-length immunoglobulin “heavy chains” (about 50 Kd or 446 amino acids) are encoded by a variable region gene (encoding about 116 amino acids) and a gamma, mu, alpha, delta, or epsilon constant region gene (encoding about 330 amino acids), the latter defining the antibody's isotype as IgG, IgM, IgA, IgD, or IgE, respectively.
  • variable and constant regions are joined by a “J” region of about 12 or more amino acids, with the heavy chain also including a “D” region of about 10 more amino acids.
  • An immunoglobulin light or heavy chain variable region (also referred to herein as a “light chain variable domain” (“VL domain”) or “heavy chain variable domain” (“VH domain”), respectively) consists of a “framework” region interrupted by three hypervariable regions, also called “complementarity determining regions” or “CDRs.”
  • the framework regions serve to align the CDRs for specific binding to an epitope of an antigen.
  • the term “hypervariable region” or “CDR” refers to the amino acid residues of an antibody that are primarily responsible for antigen binding.
  • both VL and VH domains comprise the following framework (FR) and CDR regions: FR1, CDR1, FR2, CDR2, FR3, CDR3, FR4.
  • FR framework
  • CDR regions FR1, CDR1, FR2, CDR2, FR3, CDR3, FR4.
  • the assignment of amino acids to each domain is in accordance with the definitions of Kabat, Sequences of Proteins of Immunological Interest (National Institutes of Health, Bethesda, Md., 1987 and 1991), or Chothia & Lesk, J. Mol. Biol. 196:901-917, 1987; Chothia et al., Nature 342:878-883, 1989.
  • Kabat also provides a widely used numbering convention (Kabat numbering) in which corresponding residues between different heavy chains or between different light chains are assigned the same number.
  • CDRs 1, 2, and 3 of a VL domain are also referred to herein, respectively, as CDR-L1, CDR-L2, and CDR-L3;
  • CDRs 1, 2, and 3 of a VH domain are also referred to herein, respectively, as CDR-H1, CDR-H2, and CDR-H3.
  • the term “monoclonal antibody” as used herein is not limited to antibodies produced through hybridoma technology.
  • the term “monoclonal antibody” refers to an antibody that is derived from a single clone, including any eukaryotic, prokaryotic, or phage clone, and not the method by which it is produced.
  • chimeric antibody refers to an antibody having variable domains derived from a first species and constant regions derived from a second species.
  • Chimeric immunoglobulins or antibodies can be constructed, for example by genetic engineering, from immunoglobulin gene segments belonging to different species.
  • the term “humanized antibody,” as defined infra, is not intended to encompass chimeric antibodies. Although humanized antibodies are chimeric in their construction (i.e., comprise regions from more than one species of protein), they include additional features (i.e., variable regions comprising donor CDR residues and acceptor framework residues) not found in chimeric immunoglobulins or antibodies, as defined herein.
  • humanized VH domain refers to an immunoglobulin VH or VL domain comprising some or all CDRs entirely or substantially from a non-human donor immunoglobulin (e.g., a mouse or rat) and variable region framework sequences entirely or substantially from human immunoglobulin sequences.
  • the non-human immunoglobulin providing the CDRs is called the “donor” and the human immunoglobulin providing the framework is called the “acceptor.”
  • humanized antibodies may retain non-human residues within the human variable domain framework regions to enhance proper binding characteristics (e.g., mutations in the frameworks may be required to preserve binding affinity when an antibody is humanized).
  • a “humanized antibody” is an antibody comprising one or both of a humanized VH domain and a humanized VL domain. Immunoglobulin constant region(s) need not be present, but if they are, they are entirely or substantially from human immunoglobulin constant regions.
  • a CDR in a humanized antibody is “substantially from” a corresponding CDR in a non-human antibody when at least 60%, at least 85%, at least 90%, at least 95% or 100% of corresponding residues (as defined by Kabat) are identical between the respective CDRs.
  • the CDRs of the humanized VH or VL domain have no more than six (e.g., no more than five, no more than four, no more than three, no more than two, or nor more than one) amino acid substitutions across all three CDRs relative to the corresponding non-human VH or VL CDRs.
  • variable region framework sequences of an antibody VH or VL domain or, if present, a sequence of an immunoglobulin constant region are “substantially from” a human VH or VL framework sequence or human constant region, respectively, when at least 85%, at least 90%, at least 95%, or 100% of corresponding residues defined by Kabat are identical.
  • all parts of a humanized antibody, except possibly the CDRs are entirely or substantially from corresponding parts of natural human immunoglobulin sequences.
  • Specific binding of an antibody to its target antigen means an affinity of at least 10 6 , 10 7 , 10 8 , 10 9 , or 10 10 M ⁇ 1 . Specific binding is detectably higher in magnitude and distinguishable from non-specific binding occurring to at least one unrelated target. Specific binding can be the result of formation of bonds between particular functional groups or particular spatial fit (e.g., lock and key type) whereas nonspecific binding is usually the result of van der Waals forces. Specific binding does not, however, necessarily imply that a monoclonal antibody binds one and only one target.
  • amino acid residues corresponding to those specified by SEQ ID NO includes post-translational modifications of such residues.
  • An antibody-drug conjugate is an antibody conjugated to a cytotoxic drug typically via a linker.
  • the linker may comprise a cleavable unit or may be non-cleavable.
  • Cleavable units include, for example, disulfide containing linkers that are cleavable through disulfide exchange, acid-labile linkers that are cleavable at acidic pH, and linkers that are cleavable by hydrolases, esterases, peptidases, and glucoronidases (e.g., peptide linkers and glucoronide linkers).
  • Non-cleavable linkers are believed to release drug via a proteolytic antibody degradation mechanism.
  • liquid refers to a solution suitable for altering or achieving an exemplary or appropriate concentration or concentrations as described herein.
  • container refers to something into which an object or liquid can be placed or contained, e.g., for storage (for example, a holder, receptacle, vessel, or the like).
  • administration route includes art-recognized administration routes for delivering a therapeutic protein such as, for example, parenterally, intravenously, intramuscularly, or subcutaneously.
  • administration into the systemic circulation by intravenous or subcutaneous administration may be desired.
  • administration can be localized directly into the tumor.
  • Two amino acid sequences have “100% amino acid sequence identity” if the amino acid residues of the two amino acid sequences are the same when aligned for maximal correspondence. Sequence comparisons can be performed using standard software programs such as those included in the LASERGENE bioinformatics computing suite, which is produced by DNASTAR (Madison, Wis.). Other methods for comparing two nucleotide or amino acid sequences by determining optimal alignment are well-known to those of skill in the art. (See, e.g., Peruski and Peruski, The Internet and the New Biology: Tools for Genomic and Molecular Research (ASM Press, Inc. 1997); Wu et al.
  • Two amino acid sequences are considered to have “substantial sequence identity” if the two sequences have at least 80%, at least 85%, at least 90%, or at least 95% sequence identity relative to each other.
  • Percentage sequence identities are determined with antibody sequences maximally aligned by the Kabat numbering convention. After alignment, if a subject antibody region (e.g., the entire variable domain of a heavy or light chain) is being compared with the same region of a reference antibody, the percentage sequence identity between the subject and reference antibody regions is the number of positions occupied by the same amino acid in both the subject and reference antibody region divided by the total number of aligned positions of the two regions, with gaps not counted, multiplied by 100 to convert to percentage.
  • a subject antibody region e.g., the entire variable domain of a heavy or light chain
  • compositions or methods “comprising” one or more recited elements may include other elements not specifically recited.
  • Reference to a numerical range herein includes the endpoints defining the range and all values falling within the range.
  • a benzodiazepine antibody-drug conjugate refers to an antibody conjugated to a benzodiazepine dimer typically, although not necessarily, via a linker.
  • a benzodiazepine drug-linker refers to a benzodiazepine dimer attached to a linker.
  • a benzodiazepine compound has at its core a benzene ring fused to a diazepine ring. Exemplary ring structures for the benzene ring fused to a diazepine ring are as follows:
  • Benzodiazepine compounds differ in the number, type and position of substituents on both rings and in the degree of saturation of the diazepine ring. They also differ in the number of additional rings fused to the benzene and/or diazepine ring. Included within the definition of benzodiazepine compounds are those in which the benzene or diazepine ring is fused to one or more aromatic or non-aromatic carbocyclic or heterocyclic rings.
  • a benzodiazepine dimer is a compound that has been formed by joining two benzodiazepine units together, via a tether.
  • the antibody component of the benzodiazepine antibody-drug conjugate can be conjugated to one or more benzodiazepine drug-linkers e.g., 1 to 20 drug-linkers.
  • the antibody component of the benzodiazepine antibody-drug conjugate will be conjugated to 1, 2, 3, or 4 drug-linkers. Conjugation can be via different positions on the antibody. In some aspects, conjugation will be via a sulfur atom of a cysteine residue. In some aspects the cysteine residue is a cysteine residue of the interchain disulfides of the antibody. In other aspects, the cysteine residue is engineered into the antibody.
  • the cysteine residue is engineered into the antibody at position 239 (human IgG1) as determined by the EU index (Kabat, Sequences of Proteins of Immunological Interest (National Institutes of Health, Bethesda, Md., 1987 and 1991)).
  • EU index Kabat, Sequences of Proteins of Immunological Interest (National Institutes of Health, Bethesda, Md., 1987 and 1991)
  • the benzodiazepine dimer is a pyrrolobenzodiazepine (PBD) dimer.
  • PBDs are of the general structure:
  • PBDs differ in the number, type and position of substituents, in both their aromatic A rings and pyrrolo C rings, and in the degree of saturation of the C ring.
  • the B-ring there is either an imine (N ⁇ C), a carbinolamine (NH—CH(OH)), or a carbinolamine ether (NH—CH(OR)) at the N10-C11 position, which is the electrophilic centre responsible for alkylating DNA.
  • All of the known natural products have an (S)-configuration at the chiral C11a position which provides them with a right-handed twist when viewed from the C ring towards the A ring.
  • PBDs to form an adduct in the minor groove enables them to interfere with DNA processing, hence their use as antitumour agents.
  • the biological activity of these molecules can be potentiated by joining two PBD units together, (e.g., through C8/C′-hydroxyl functionalities via a flexible alkylene linker).
  • the PBD dimers are thought to form sequence-selective DNA lesions such as the palindromic 5′-Pu-GATC-Py-3′ interstrand cross-link which is thought to be mainly responsible for their biological activity.
  • PBD dimers to be used as conjugates are as follows:
  • the PBD dimer can be linked to the antibody at any position suitable for conjugation to a linker.
  • the PBD dimer will have a substituent at the C2 position (e.g., a primary or secondary amine) that provides an anchor for linking the compound to the antibody.
  • the C2 position is marked by an arrow in the exemplary structures shown above.
  • the N10 position of the PBD dimer will provide the anchor for linking the compound to the antibody.
  • the benzodiazepinedimer drug is an indolinobenzodiazepine dimer or an oxazolidinobenzodiazepine dimer.
  • IBDs Indolinobenzodiazepines
  • OBDs oxazolidinobenzodiazepines
  • Indolinobenzodiazepines and oxazolidinobenzodiazepines differ in the number, type and position of substituents in their rings.
  • two indolinobenzodiazepines or two oxazolidinobenzodiazepines units can be joined together to form dimers, e.g., through ether functionalities between the A rings of two monomeric units.
  • an indolinobenzodiazepine dimer or oxazolidinobenzodiazepine dimer can be linked to an antibody at any position suitable for conjugation to a linker.
  • a benzodiazepine ADC that comprises a PBD dimer as the drug component can also be referred to as a PBD ADC.
  • a benzodiazepine ADC that comprises an indolinobenzodiazepine dimer as the drug component can be referred to as an IBD ADC and an ADC that comprises an oxazolidinobenzodiazepine dimer as the drug component can be referred as an OBD ADC.
  • benzodiazepine ADCs including PBD ADCs, IBD ADCs and OBD ADCs, comprise a linker between the benzodiazepine drug and the antibody.
  • the linker may comprise a cleavable unit (e.g., an amino acid or a contiguous sequence of amino acids that is a target substrate for an enzyme) or a non-cleavable linker (e.g., linker released by degradation of the antibody).
  • the linker may further comprise a maleimide group for linkage to the antibody, e.g., maleimidocaproyl.
  • PBD dimers, IBD dimers, OBD dimers, linkers, and conjugates thereof are known in the art. See for example, WO 2010/091150, WO 2012/112708, WO 2012/128868, WO 2011/023883, and WO 2009/016516.
  • An exemplary linker for use with the benzodiazepine drugs is as follows wherein the wavy line indicates the site of attachment to the drug and the antibody is linked via the maleimide group.
  • Exemplary PBD-based antibody-drug conjugates include antibody-drug conjugates as shown below:
  • Ab is an antibody and drug-loading is represented by p, the number of drug-linker molecules per antibody.
  • p can represent the average number of drug-linker molecules per antibody, also referred to the average drug loading.
  • P ranges from 1 to 20 and is preferably from 1 to 8.
  • when p represents the average drug loading p ranges from about 2 to about 5.
  • p is about 2, about 3, about 4, or about 5.
  • the antibody is conjugated to the drug linker via a sulfur atom of a cysteine residue.
  • the cysteine residue is a cysteine residue of the interchain disulfides of the antibody.
  • cysteine residue is engineered into the antibody.
  • the cysteine residue is engineered into the antibody at position 239 (IgG1) as determined by the EU index (Kabat, Sequences of Proteins of Immunological Interest (National Institutes of Health, Bethesda, Md., 1987 and 1991)).
  • EU index Kabat, Sequences of Proteins of Immunological Interest (National Institutes of Health, Bethesda, Md., 1987 and 1991)).
  • Methods of making such ADCs are known in the art (see, for example, International Publication No. WO2011/130613).
  • the present invention is directed, inter alia, to methods for the removal of benzodiazepine drug-related impurities from a mixture comprising benzodiazepine ADCs and benzodiazepine-related impurities.
  • a benzodiazepine drug-related impurity is any drug-related impurity arising from the conjugation reaction (including quenching step) of an antibody to a benzodiazepine drug or drug-linker.
  • Benzodiazepine drug-related impurities include, for example, benzodiazepine dimer free drugs (including quenched drug), benzodiazepine drug-linkers, quenched benzodiazepine drug-linkers, or benzodiazepine drug-linker degradation products.
  • Benzodiazepine drug-related impurities do not include benzodiazepine drugs or drug-linkers conjugated to antibodies (including antibody containing fragments thereof).
  • an antibody naked or conjugated to a linker
  • a benzodiazepine drug-linker under conditions sufficient to form a conjugation reaction mixture comprising benzodiazepine ADCs and benzodiazepine drug-related impurities.
  • an antibody e.g., antibody-linker
  • a benzodiazepine free drug under conditions sufficient to form a conjugation reaction mixture comprising benzodiazepine ADCs and benzodiazepine drug-related impurities.
  • conjugation will be to the antibody's lysine residues.
  • conjugation will be to a native or engineered cysteine present on the antibody (e.g., cysteine of an inter-chain disulfide or cysteine residue introduced into the heavy or light chain of the antibody).
  • the engineered cysteine antibody will be reduced prior to contact with the benzodiazepine drug-linker, the antibody will be partially re-oxidized (i.e., re-oxidized as to the inter-chain disulfides but not as to the introduced cysteine) and the benzodiazepine drug-linker will be conjugated to the engineered cysteine on the partially re-oxidized antibody.
  • the engineered cysteine will be at position 239 (IgG1, EU index numbering as set forth in Kabat).
  • conjugation reactions are conducted at a temperature of from about 0° C. to about 40° C. In some embodiments, the conjugation reaction is conducted at about 4° C. In some embodiments, the conjugation reaction is conducted at about 25° C. In some embodiments, the conjugation reaction is conducted at about 37° C.
  • the conjugation reactions can be conducted for any suitable length of time. In general, the conjugation reaction mixtures are incubated under suitable conditions for anywhere between a few minutes and several hours.
  • the reactions can be conducted, for example, for about 1 minute, or about 5 minutes, or about 30 minutes, or about 11 ⁇ 2 hours, or about 4 hours, or about 12 hours, or about 24 hours.
  • conjugation reaction mixtures are formed with a pH ranging from about 6 to about 9.
  • the reaction mixture is formed with a pH of about 7 to about 8.
  • Various buffering agents can be used to maintain a particular pH.
  • buffering agents include, but are not limited to, 2-(N-morpholino)ethanesulfonic acid (MES), 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (HEPES), 3-morpholinopropane-1-sulfonic acid (MOPS), 2-amino-2-hydroxymethyl-propane-1,3-diol (TRIS), sodium citrate, sodium acetate, and sodium borate.
  • MES 2-(N-morpholino)ethanesulfonic acid
  • HPES 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
  • MOPS 3-morpholinopropane-1-sulfonic acid
  • TMS 2-amino-2-hydroxymethyl-propane-1,3-diol
  • Cosolvents e.g., dimethyl acetamide, propylene glycol, dimethylsulfoxide, dimethylformamide, ethanol, methanol, tetrahydrofuran, acetone, and acetic acid
  • salts e.g., NaCl, KCl, CaCl 2 , and salts of Mn 2+ and Mg 2+
  • chelators e.g., ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA), 2-( ⁇ 2-[Bis(carboxymethyl)amino]ethyl ⁇ (carboxymethyl)amino)acetic acid (EDTA), and 1,2-bis(o-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid (BAPTA)) can also be included as necessary.
  • EGTA ethylene glycol-bis(2-aminoethylether)-
  • Buffers, cosolvents, salts, and chelators can be used at any suitable concentration, which can be readily determined by one of skill in the art.
  • buffers, cosolvents, salts, and chelators can be included in reaction mixtures at concentrations ranging from about 1 ⁇ M to about 1M or even higher (e.g., 0-50% v/v depending on the co-solvent). Any suitable amount of benzodiazepine drug or drug-linker can be used for conjugation.
  • a quenching agent will be used to quench any excess drug or drug-linker (e.g., unconjugated drug or drug-linker).
  • a quenching agent is a reagent, other than an antibody, that is capable of abolishing the reactivity of a reactive moiety by covalently binding to the reactive moiety.
  • the quenching agent will be chosen based on the nature of the drug or linker. For example, a thiol such as ⁇ -mercapto ethanol or N-acetylcysteine can be used to quench excess drug-linker compounds containing a maleimido group.
  • An amine such as glycine can be used to quench excess linker-drug compounds containing an N-hydroxysuccinimidyl ester.
  • the quenching agent is used in excess with respect to the antibody and the drug-linker.
  • excess benzodiazepine drug-linker is present in the reaction mixture and will be quenched.
  • the benzodiazepine drug-related impurity will be a quenched benzodiazepine drug-linker.
  • a quenched conjugation reaction mixture refers to a reaction mixture following conjugation of the antibody to the drug-linker and introduction of the quenching agent.
  • WO 2011/130613 describes a method of synthesizing a PBD drug-linker followed by conjugating the PBD drug-linker to an antibody. Briefly, antibodies in PBS containing 50 mM sodium borate at pH 7.4 are reduced with tris(carboxyethyl)phosphine hydrochloride (TCEP) at 37° C. Antibody inter-chain disulfides are reformed by oxidation with dehydroascorbic acid, leaving the engineered cysteines in the thiol form available for alkylation with drug linker.
  • TCEP tris(carboxyethyl)phosphine hydrochloride
  • the reduced antibody is then alkylated with ⁇ 1.5 equivalents of maleimide drug-linker per antibody thiol, in the presence of sufficient co-solvent to solubilize the drug-linker. After about 90 min, the reaction is quenched by the addition of ⁇ 3 equivalents of N-acetyl cysteine relative to drug-linker.
  • benzodiazepine drug-related impurities will be present in the mixture. Generally, benzodiazepine drug-related impurities will be present in the mixture at levels of about 10 to 100 ⁇ M. Higher or lower concentrations of benzodiazepine drug-related impurities are contemplated.
  • the benzodiazepine process impurity will be a quenched or un-quenched drug-linker, e.g., N-acetyl cysteine quenched drug-linker.
  • the benzodiazepine process impurity will be a free benzodiazepine drug (i.e., benzodiazepine dimer not attached to a linker or antibody).
  • the benzodiazepine drug-related impurity will be a benzodiazepine drug-linker degradation product, such as, for example, an oxidized or hydrolyzed derivative of the drug-linker.
  • the mixture comprising benzodiazepine ADCs and benzodiazepine drug-related impurities will be filtered in order to reduce turbidity present in the process intermediate reaction mixture.
  • the filtration can be via dead-end filtration (e.g., using filters with a pore size of 1 micron or smaller) or other filtration methods, including, for example, depth filtration.
  • activated charcoal filtration will immediately follow conjugation, e.g., charcoal filtration will be the first purification step following conjugation and optional quenching.
  • Activated charcoal also known as activated carbon, is charcoal that has been chemically or thermally activated. Activated charcoal has a very high surface area to mass ratio which enables physical adsorption of solute. Activated charcoal can come in bulk powder form or attached to a solid support. In some preferred embodiments, the activated charcoal used in the present invention is attached to a solid support.
  • a solid support refers to an insoluble, functionalized material to which activated carbon can attach. Examples of solid supports include, for example, functionalized polymeric materials such as cellulose (e.g., cellulose fibers).
  • activated charcoal filtration The process of filtering a conjugation reaction mixture through an activated charcoal filter is referred to herein as activated charcoal filtration.
  • charcoal filters examples include charcoal-impregnated filters.
  • Exemplary filters include the activated carbon cartridges and capsules sold by 3M (e.g., Zeta PlusTM filters), Pall, and Millipore. Passage of the mixture comprising the benzodiazepine drug-related impurities through the activated charcoal filter is conducted to clear the benzodiapine drug-related impurities from the ADC preparation.
  • the activated charcoal used in the present invention is in bulk powder form and is added, as a powder, or suspension, to the mixture to be purified. Passage of the mixture comprising the benzodiazepine ADCs, benzodiazepine drug-related impurities and activated charcoal through a filter clears the benzodiapine drug-related impurities from the ADC preparation.
  • activated charcoal filtration is preferably by single pass filtration through a single filter, multiple discrete filtrations through a single filter, or recirculation through a single filter.
  • activated charcoal filtration is preferably by multiple discrete filtrations through a single filter or recirculation through a single filter. Accordingly in preferred embodiments of the present invention, filtration through two or more separate filters is not performed.
  • recirculation refers to passing a mixture through a filter by channeling the outlet stream from the filter directly to the inlet of the same filter, or to a reservoir, in which the reaction mixture is contained until the filtration process is complete. The stream can be channeled via an appropriate apparatus, such as a tube or pipe.
  • benzodiazepine ADC protein
  • benzodiazepine drug-related impurities e.g., about 1, 2, 3, 4, 5, 6, 7, 8, or 9 g benzodiazepine ADC in the ADC mixture
  • recirculation through a single filter is preferred as compared to single pass filtration.
  • single pass filtration is preferred.
  • benzodiazepine ADC when there is greater than 10 g benzodiazepine ADC, or greater than 20 g benzodiazepine ADC in the mixture comprising benzodiazepine ADCs and benzodiazepine drug-related impurities, multiple discrete filtration or recirculation is preferred as compared to single pass filtration.
  • Select parameters can be varied to alter the level of benzodiazepine drug-related impurities that can be removed from a mixture comprising them. It is known that the adsorption of solutes by carbon filtration is generally dependent on contact time, so that extraction efficiency depends on flux. Flow rate of the mixture through the activated charcoal filter can be increased or decreased as necessary to achieve a desired level of purity. In some aspects, the flow rate though the activated charcoal filter will be a flux of about 10 L/min/m 2 or lower, about 8 L/min/m 2 or lower, or about 6 L/min/m 2 .
  • the flux will be between about 3 L/min/m 2 , or about 6 L/min/m 2 to about 10 L/min/m 2 or about 20 L/min/m 2 . In some aspects, the flux will be between about 6 L/min/m 2 to about 10 L/min/m 2 . In some aspects, the flux will be between about 6 L/min/m 2 to about 8 L/min/m 2 .
  • the increase in flux can be compensated for by increasing the number of recirculation cycles.
  • a flow rate of 12 L/min/m 2 might require 6 or more complete passages through a single filter.
  • the reaction mixture will be recirculated from 2 to about 20 times over the activated charcoal filter, preferably from 5 to about 15 times over the activated charcoal filter.
  • the filtration process will take from about 1 hour to about 5 hours, preferably from about 2 hours to about 4 hours to reduce the benzodiazepine process intermediates to an acceptable level. In some aspects, the filtration process will be completed when the concentration of benzodiazepine drug-related impurities has reached a target level. In some aspects, a target clearance level is about 1 ⁇ M or less, preferably 0.2 ⁇ M, or even 0.1 ⁇ M or less.
  • activated charcoal filtration will reduce the levels of benzodiazepine drug-related impurities (e.g., quenched drug-linkers) in the final reaction mixture to about 1 ⁇ M or less, preferably 0.2 ⁇ M, or even 0.1 ⁇ M or less.
  • benzodiazepine drug-related impurities e.g., quenched drug-linkers
  • the present invention provides benzodiazepine ADC formulations comprising 0.2 ⁇ M or less, 0.1 ⁇ M or less or 0.05 ⁇ M or less benzodiazepine drug-related impurities.
  • the present methods can be used for both small scale manufacturing efforts and large-scale efforts.
  • the present methods are applicable to a range of scales from milligrams to kilograms.
  • additional purification methods including tangential flow filtration can be used to remove additional process-related impurities, including the non drug-related process impurities, e.g., chelators, solvents, quenching agents, and the like.
  • FIGS. 1, 2, and 3 were conducted using Grades 3 or 5 3M Purification Zeta Plus 13.5 cm 2 activated charcoal discs mounted in a stainless steel holder.
  • Table 1 and FIG. 4 were conducted using 3M Purification Zeta Plus BC25 Grade 5 Activated Charcoal Capsule Filters.
  • Experiments in FIG. 5 were conducted using Zeta Plus Activated Carbon Capsule Filters, BC25 or BC1000 (650 cm 2 ) as indicated in the graph legend. Protein loading is in the range of 700 g/m 2 or less, and flux was ⁇ 6 LMM or as indicated.
  • the carbon filters were used according to the vendor's instructions. Carbon filters were flushed prior to use to clear unsequestered carbon powder, then equilibrated with the QCR buffer medium (50 mM Tris/5 mM EDTA, pH 7.5). Flow of the QCR was maintained by a peristaltic pump. Effluent was sampled from the flow immediately after passing through the filter.
  • the QCR buffer medium 50 mM Tris/5 mM EDTA, pH 7.5.
  • Benzodiazepine drug-related impurities were quantified by RP-HPLC. Drug-related peaks were detected and quantified by UV.
  • PBD dimers 1-4 and the synthesis thereof are described in WO2011/130613.
  • PBD dimers 5-10 and 16 can be synthesized using the methods described in WO2011/130613 A1. Briefly, PBDs dimers 9 and 16 are accessible through the C3-tethered bis-triflate intermediate 8a in WO2011/130613 A1. The desired C2 aryl groups as boronic acids or pinacol boronates are introduced in sequential Suzuki couplings, followed by SEM dilactam reduction to reveal the imine functional groups.
  • PBD dimers 5-8 and 10 are prepared in the same manner from C5-tethered bis-triflate intermediate 8b in WO2011/130613 A1.
  • PBD dimers 11-15 containing esters or carboxylic acids in the C2 aryl groups can be accessed using the methods described in WO2011/130613 A1 with minor modifications.
  • PBD dimer 13 can be prepared from the C3-tethered bis-triflate intermediate 8a in WO2011/130613 A1.
  • the bis triflate is desymmetrized via Suzuki coupling with an appropriately functionalized boronic acid or pinacol boronate to install the C2 aryl group bearing the amino functional group.
  • the resulting monotriflate is then reduced with lithium triethylborohydride to the SEM carbinol, which is then carried forward to the second Suzuki coupling to install the C2 aryl group containing the methyl ester.
  • SEM carbinols are converted to imines via stirring on silica gel for 3 days, as described in WO2011/130613 A1.
  • PBD dimers 12, and 14 can be prepared in the same way starting with C5-tethered bis triflate 8b in WO2011/130613 A1. Conversion of the PBD esters to the free carboxylic acids (11 and 15) could be achieved via saponification.
  • the quenched drug-linker referred to in the following examples has the following structure:
  • the 2H12 antibody is an engineered IgG1 antibody having a cysteine residue substitution at position 239 of the heavy chain.
  • the sequence of the 2H12 heavy and light chain variable regions and constant regions are provided in SEQ ID NOs:1-4.
  • the quenched conjugation reaction mixture (QCR) used in the following filtration experiments was prepared as follows: Anti-CD33 antibody, h2H12 IgG1, having an introduced cysteine at position 239 (EU index numbering) was reduced, partially re-oxidized (i.e., re-oxidized as to inter-chain disulfides), and conjugated to the PBD drug-linker using methods described in WO 2011/130613 to form an ADC. The PBD drug-linker was conjugated to the partially re-oxidized antibody via the introduced cysteine residues (average of 2 drug-linkers per antibody). After conjugation, the reaction mixture was quenched by the addition of N-acetyl cysteine and filtered using dead-end filtration.
  • the quenched drug-linker was purified from the QCR using constant volume diafiltration.
  • the quenched conjugation reaction mixture was introduced into the tangential flow filtration device.
  • the quenched conjugation reaction mixture comprises of Tris, NaCl and 50% propylene glycol.
  • the tangential flow filtration buffer also comprises Tris, NaCl and 50% propylene glycol.
  • Activated charcoal filters 25 cm 2 3M Purification Zeta Plus BC25 Grade 5 activated Charcoal Capsule filter
  • the concentration of quenched drug-linker remained constant throughout the filtration ( FIG. 1 ).
  • the QCR contained 35.9 ⁇ M quenched drug-linker demonstrating that carbon filtration reduced the level of quenched drug-linker by about 98%. Determination of protein concentration at the beginning and end of this filtration indicated a loss of approximately 7% of the ADC.
  • recirculation through a single filter helped clear quenched drug-linker to levels less than 0.1 ⁇ M when using a BC25 filter (protein loading concentrations of less than 10 g) or a BC1000 filter (protein loading concentration of greater than 10 g).
  • a BC25 filter protein loading concentrations of less than 10 g
  • a BC1000 filter protein loading concentration of greater than 10 g
  • one pass was sufficient to reduce quenched drug-linker levels to less than 0.1 ⁇ M.

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