US5576187A - Standards for phosphorothioate insecticide immunoassays - Google Patents
Standards for phosphorothioate insecticide immunoassays Download PDFInfo
- Publication number
- US5576187A US5576187A US08/303,343 US30334394A US5576187A US 5576187 A US5576187 A US 5576187A US 30334394 A US30334394 A US 30334394A US 5576187 A US5576187 A US 5576187A
- Authority
- US
- United States
- Prior art keywords
- compound
- standardizing
- alkyl
- integer
- och
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/967—Standards, controls, materials, e.g. validation studies, buffer systems
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/815—Test for named compound or class of compounds
Definitions
- the invention relates to improvements in immunoassays for phosphorothioate insecticides, such as chlorpyrifos.
- Chlorpyrifos and structurally related compounds have insecticidal activity against a variety of arthropods. Because of their extensive use in agriculture, it is important to be able to monitor their levels in water and food that could be ingested by humans. To that end, immunoassays utilizing antibodies against such phosphorothioate compounds as chlorpyrifos, chlorpyrifos-methyl, fenitrothion, and pirimiphos-methyl have been developed (J.H. Skerrit et al, Journal of AOAC International, Vol 75, 519 (1992); J. H. Skerrit et al; abstracts of The 106th AOAC Annual International Meeting & Exposition, Cincinnati, Ohio, 1992. p.162; PCT application PCT/AU90/00278 of J. Skerrit et al).
- the invention relates to immunoassays involving antibodies that react with chlorpyrifos or compounds structurally related to chlorpyrifos, the inventive improvement being the use of stable compounds as antibody-reactive standards in such assays.
- stable compounds are diethylchlorothiophosphate, diethyl 4-methylbenzylphosphonate, and N-methyl-3,5,6-trichloro-2-pyridinone, or similar compounds.
- kits comprising the stable standards.
- FIG. 1 Immunoassay standardization curves.
- FIG. 2 Reactivity of N-methyl-3,5,6-trichloro-2-pyridinone in the immunoassay as a function of time and temperature of storage of that compound.
- FIG. 3 Reactivity of diethyl 4-methylbenzylphosphonate in the immunoassay as a function of time and temperature of storage of that compound.
- FIG. 4 Reactivity of the compound, diethylchlorothiophosphate, in the immunoassay as a function of time and temperature of storage of that compound.
- FIG. 5 Reactivity of the compound, chlorpyrifos, in the immunoassay as a function of time and temperature of storage of that compound.
- the invention is an immunoassay process comprising the steps of:
- a sample preferably a solution, and more preferably an aqueous solution
- an antibody reacting a sample (preferably a solution, and more preferably an aqueous solution) with an antibody, said sample comprising an amount of analyte, said antibody reactive against said analyte,
- step (2) 2) reacting a known amount of a standardizing compound (preferably in solution, and more preferably in an aqueous solution) with an antibody of the same specificity as that used in step (1),
- step 5) utilizing the amounts quantitated in steps (3) and (4) and the known amount in step (2) to calculate the amount of analyte present in the sample used in step (1), wherein the analyte is ##STR1## wherein the standardizing compound is either ##STR2## wherein X 1 x is C or N,
- X 2 is C if X l is C
- X 2 is C or N if X l is N,
- R is C 1 -C 4 alkyl
- R 2 is H, C 1 -C 4 alkyl, NO 2 , a dialkylamino that is di(C 1 -C 4 alkyl)amino, Cl, Br, I, (C 1 -C 4 alkyl)--S--, C 1 -C 4 alkyl-(O ⁇ S)--, --SO 2 NH 2 , or --S--R 5 , provided that R 2 is --S--R 5 only if X 1 and X 2 are both C and R 2 is in the para position (4-position on the benzene ring),
- R 3 is H, Cl, Br, or I
- R 4 is H, Cl, Br, or I
- R 5 is ##STR3## wherein R 6 is C 1 -C 4 alkyl
- R 7 is Cl, Br, I, OH, (CH 2 ), m CH 3 , benzyl or benzyl substituted with I, Cl, Br, CH 3 , OCH 3 , O(CH2) m CH 3 , and m is an integer in the range 1 through 10 (i.e., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10)
- R 8 is O, NH, or S
- R 9 is H, or C 1 -C 4 alkyl
- R 10 is Cl, Br, I, CH 3 , (CH 2 ) q CH 3 , OCH 3 , or O(CH 2 ) q CH 3 , and q is an integer in the range 1 through 10,
- R 11 is (CH 2 ) y , and y is an integer in the range 1 through 10
- R 12 is O, NH, or S
- R 13 is CH 3 , (CH 2 ) z CH 3 ,OCH 3 , or O(CH 2 ) z CH 3 and wherein z is an integer in the range 1 through 10,
- R 14 is Cl, I or Br,
- R 15 is H, Cl, Br, or I
- R 16 is H, Cl, Br, or I
- R 2 , R 3 , and R 4 are not joined to X 1 if it is N or to X 2 if it is N. Otherwise, the foregoing text and diagrams in this Description section of the application are not intended to limit the positions of the three substituents, R 2 , R 3 , and R 4 , to any particular position or positions on the 6-atom aromatic ring to which they are linked, except that not more than one of those three substituents can be at any given atom on the ring on a single compound, and of course none of those substituents can be at the same position occupied by the --O--PS(OR 1 ) 2 group.
- the invention is a process of calibrating a standardizing compound relative to a test compound of interest in an immunoassay of interest, said process comprising the steps of:
- step (2) reacting under the immunoassay conditions of step (1) a known amount of a standardizing compound with an antibody of the same specificity as that used in step (1) (i.e., same immunoassay conditions as step (1) except that the test compound has been replaced with the standardizing compound),
- test compound is ##STR4## wherein the standardizing compound is either ##STR5## wherein X 1 is C or N,
- X 2 is C if X 1 is C
- X 2 is C or N if X 1 is N
- R 1 is C 1 -C 4 alkyl
- R 2 is H, C 1 -C 4 alkyl, NO 2 , a dialkylamino that is di(C 1 -C 4 alkyl)amino, Cl, Br, I, (C 1 -C 4 alkyl)--S--, C 1 -C 4 alkyl-(O ⁇ S)--, --SO 2 NH 2 , or --S--R 5 , provided that R 2 is --S--R 5 only if X 1 and X 2 are both C and R 2 is in the para position (4-position on the benzene ring)
- R 3 is H, Cl, Br, or I
- R 4 is H, Cl, Br, or I
- R 5 is ##STR6## wherein R 6 is C 1 -C 4 alkyl
- R 7 is Cl, Br, I, OH, (CH 2 ) m CH 3 , benzyl or benzyl substituted with I, Cl, Br, CH 3 , OCH 3 , O(CH2) m CH 3 , and m is an integer in the range 1 through 10 (i.e., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10)
- R 8 is 0, NH, or S
- R 9 is H or C 1 -C 4 alkyl
- R 10 is Cl, Br, I, CH 3 , (CH 2 ) q CH 3 , OCH 3 , or O(CH 2 ) q CH 3 , and q is an integer in the range 1 through 10,
- R 11 is (CH 2 ) y , and y is an integer in the range 1 through 10
- R 12 is O, NH, or S
- R 13 is CH 3 , (CH 2 ) z CH 3 , OCH 3 , or O(CH 2 ) z CH 3 and wherein z is an integer in the range 1 through 10,
- R 14 is Cl, I or Br,
- R 15 is H, Cl, Br, or I
- R 16 is H, Cl, Br, or I
- R 2 , R 3 , and R 4 are not joined to X 1 if it is N or to X 2 if it is N.
- R 2 is dialkylamino then it be used when X 1 and X 2 are both N and that it be at that carbon on the aromatic ring immediately adjacent to both N atoms.
- R 2 is NO 2 , C 1 -C 4 alkyl-S--, C 1 -C 4 alkyl-(O ⁇ S)--, --SO 2 NH 2 , that X 1 and X 2 both be C and that R 2 be at the 4-position on the aromatic ring; in those cases it is even more preferred that R 3 and R 4 both be H.
- Particularly preferred standardizing compounds are N-methyl-3,5,6-trichloro-2-pyridinone, diethyl 4-methylbenzylphosphonate, and diethylchlorothiophosphate.
- N-methyl-3,5,6-trichloro-2-pyridinone is a compound described above with R 13 and R 14 and wherein R 13 is methyl and R 14 is Cl. It was obtained from DowElanco, Indianapolis, Ind.
- Diethyl 4-methylbenzylphosphonate is a compound described above with R 9 , R 10 , R 11 , and R 12 , and wherein R 9 is ethyl, R 10 is methyl, R 11 is CH 2 , R 12 is O and R 15 and R 16 are H. It is commercially available from Lancaster Synthesis, Windham, N.H.
- Diethylchlorothiophosphate is the compound above with R 6 , R 7 , and R 8 , and wherein R 6 is ethyl, R 7 is Cl and R 8 is S. It is commercially available from Aldrich Chemical Company, Milwaukee, Wis.
- C 1 -C 4 alkyl includes methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl, isobutyl, and tert-butyl.
- An example of "(C 1 -C 4 )alkyl S--" is "--SCH 2 CH 3 ".
- C 1 -C 4 alkyl-(O ⁇ S)-- the C 1 -C 4 alkyl group is directly linked to the sulfur atom and the sulfur atom is also directly linked to both the oxygen atom and the aromatic ring of the analyte or test compound.
- R 3 is Cl, Br, or I
- both R 2 and R 4 are either H, Cl, Br, or I. It is more preferred that if R 3 is Cl, Br, or I, then R 2 , R 3 , and R 4 be the same.
- R 15 is Cl, Br, or I
- both R 10 and R 16 are either H, Cl, Br, or I. It is more preferred that if R 15 is Cl, Br, or I, then R 10 , R 15 , and R 16 be the same.
- Preferred analyte and test compounds are: chlorpyrifos, chlorpyrifos-methyl, diazinon, fenitrothion, parathion-methyl, parathion, fensulfothion, fenthion, pyrimiphos-ethyl, pyrimiphos-methyl, dicapthon, cythioate, and temephos.
- Chlorpyrifos is O,O-diethyl O-(3,5,6-trichloro-2-pyridyl)phosphorothioate (CAS: 2921-88-2).
- Chlorpyrifos-methyl is O,O-dimethyl O-(3,5,6-trichloro-2-pyridyl)phosphorothioate. (CAS: 598-13-0).
- Diazinon is O,O-odiethyl O-isopropyl-6-methylpyfimidin-4-yl phosphorothioate (CAS:333-41-5).
- Fenitrothion is O,O-dimethyl O-(3-methyl-4-nitrophenyl)phosphorothioate (CAS: 122-14-5).
- Parathion-methyl is O,O-dimethyl O-4-nitrophenyl phosphorothioate (CAS:298-00-0).
- Parathion is O,O-diethyl O-4-nitrophenyl phosphorothioate (CAS: 56-38-2).
- Fensulfothion is O,O-diethyl O-[p-(methylsulfinyl)phenyl]phosphorothioate (CAS: 115-90-2).
- Fenthion is O,O-dimethyl O-[3-methyl-4(methylthio)phenyl]phosphorothioate (CAS:55-38-9).
- Pyfimiphos-ethyl is O-2-diethylamino-6-methylpyrimidin-4-yl O,O-diethylphosphorothioate (CAS:23505-41-1).
- Pyfimiphos-methyl is O-2-diethylamino-6-methylpyfimidin-4-yl O,O-dimethylphosphorothioate (CAS: 29232-93-7).
- Dicapthon is O(2-chloro-4-nitrophenyl) O,O-dimethyl phosphorothioate (CAS: 2463-84-5).
- Cythioate is O,O-dimethyl O-p-sulfamoylphenyl phosphorothioate (CAS:115-93-5).
- a preferred way to do the assay is to first do the assay with a known amount (A c ) of freshly made chlorpyrifos as standardizing compound and also with a known amount (A sc ) of a stable standardizing compound (e.g., diethylchlorothiophosphate), such that the same signal, S, is generated by chlorpyrifos and the stable standardizing compound (e.g., In Example 1 below, the signal is the absorbance at 450 nm).
- a c a known amount of freshly made chlorpyrifos as standardizing compound
- a sc a stable standardizing compound
- the stable standardizing compound e.g., diethylchlorothiophosphate
- chlorpyrifos were stable under storage, it would not be necessary for purposes of analyzing for chlorpyrifos to also do the assay with a stable standardizing compound other than chlorpyrifos. As shown in Example 1, however, chlorpyrifos is not stable upon storage. Therefore it is better to use a stable standardizing compound (e.g., diethyl 4-methylbenzylphosphonate) as the standard when measuring a sample with unknown amounts of chlorpyrifos.
- a stable standardizing compound e.g., diethyl 4-methylbenzylphosphonate
- a related invention is a kit for an immunoassay in which the analyte is ##STR7## said kit comprising a standardizing compound that is either ##STR8##
- An antibody used in steps (1) and (2) may be a polyclonal or monoclonal antibody. If the antibody is a polyclonal preparation, then in order to use antibodies of the same specificity in both steps (1) and (2), it is preferred that portions of the same aliquot of the preparation are used for steps (1) and (2). If the antibody is a monoclonal antibody generated by a hybridoma, then in order that antibodies of the same specificity be used in steps (1) and (2), it is preferred that antibodies from that hybridoma will be used for steps (1) and step (2).
- the invention and the standardizing compounds can be used in any assay format: competition assays (as in Example 1 below) where the analyte competes with labeled chlorpyrifos for the binding sites provided by the anti-chlorpyrifos antibody; non-competition assays, where the analyte does not compete for such binding sites; sandwich assays, where one anti-analyte antibody acts as a bridge to bind the analyte to a solid phase, and a second anti-analyte antibody with a detectable label is allowed to attach to the solid phase-bound analyte; or any other immunoassay format.
- competition assays as in Example 1 below
- non-competition assays where the analyte does not compete for such binding sites
- sandwich assays where one anti-analyte antibody acts as a bridge to bind the analyte to a solid phase, and a second anti-analyte antibody with a detectable label
- Diluted samples should be kept on ice and assayed as soon as possible. All other reagents, including the standards and control, should be at room temperature prior to use. Sample dilutions should be made using Diluent/Zero Standard and mixed thoroughly prior to assaying.
- Color Reagent is prepared just prior to use by combining 1 volume of Chromogen solution with one volume of Peroxide solution.
- the Color Reagent is prepared in a clean glass container and should remain clear.
- the solution of antibody-coupled particles should not be in glass containers.
- Reagents are added directly to the bottom of a tube while avoiding contact between the reagents in the tube and the pipet tip or droplets attached to the pipet tip. Cross-contamination and carry-over of reagents is avoided by using clean pipers for each sample addition. Foam formation during vortexing is undesirable.
- a magnetic separator (e.g., the RAPID Magnetic Separator) consists of two parts: an upper rack which securely holds the test tubes and a lower part which contains magnets used to attract the antibody-coupled magnetic particles.
- the rack is described and illustrated in J.A. Itak et al, Chemosphere, vol 24, pp 11-21 (1992).
- the rack and the lower part of the separator are combined to pull the magnetic particles to the walls of the tubes. To obtain optimum assay precision, it is important to perform the separation steps carefully and consistently.
- the rack is decanted by inverting it using a smooth turning action so that the liquid flows consistently along one side of the test tube. While still inverted, the rack is placed on an absorbent pad and allowed to drain. Lifting the rack and replacing gently into the pad several times will ensure complete removal of the liquid from the rim of the tube.
- the antibody-coupled magnetic particles should be mixed just prior to pipetting.
- the assay was performed as follows:
- a 1M glycine solution was then used to quench any unreacted sites for 30 minutes.
- the particles were then washed four times with 5 ml of 25 mM Tris, 150 mM NaCl, 0.1% BSA, 15 ppm Kathon (octhilinone, Rohm & Haas), 1 mM EDTA, pH 7.4.
- Particles were diluted so as to achieve an iron concentration of 8-10 mg/ml and stored in 25 mM Tris/150 mM NaCl, 0.1% bovine serum albumin (BSA), 15 ppm Kathon, 1 mM ethylenediaminetetraacetic acid (EDTA), pH 7.4.
- BSA bovine serum albumin
- EDTA ethylenediaminetetraacetic acid
- the magnetic particles were diluted 1:1000 in 150 mM Tris, 150 mM NaCl 0.1% Gel (fish gelatin, Sigma, St. Louis, Mo., Cat #G7765), 15 ppm active Kathon, 1 mM EDTA, pH 7.4.
- a 1:100,000 dilution of a solution of anti-chlorpyrifos monoclonal antibody serum (21.6 mg/ml of antibody) was added and incubated at room temperature (about 25° C.) for at least 16 hours.
- Chlorpyrifos enzyme conjugate was chlorpyrifos conjugated to horse radish peroxidase (HRP) and was diluted to a concentration of approximately 18 ng/ml in 25 mM sodium acetate, 150 mM NaCl, 0.1 mM luminol (3-aminophthalhydrazide), 0.1% gel, 15 ppm Kathon, pH 5.0.
- HRP horse radish peroxidase
- Each standardizing compound was dissolved or diluted in methanol to obtain a concentration of 10 mg/ml and then diluted to the specified concentration in "Diluent/Zero Standard" (25 mM sodium acetate, 150 mM NaCl, 0.1% gel, 0.1 mM EDTA, 15 ppm Kathon, pH 4.0).
- Peroxide solution was a 0.02% solution of hydrogen peroxide in citric buffer, pH 4.5. (Obtained from Kirkegaard & Perry Laboratories, Gaithersburg, Md.)
- Chromogen solution was 0.4 g/ml of 3,3'5,5'tetramethylbenzidine ("TMB") in 26% dimethylformamide. (Obtained from Kirkegaard & Perry Laboratories, Gaithersberg, Md.).
- Washing Buffer was 25 mM Tris, 150 mM NaCl, 0.1% gel, 0.1% Tween, 15 ppm Kathon, pH 7.4.
- Polystyrene test tubes were used.
- Anti-chlorpyrifos antibody was prepared by using a chlorpyrifos-thyroglobulin (porcine) immunogen whose structure is: ##STR9## wherein the amine moiety on the thyroglobulin is contributed by a primary amine of that protein.
- Balb/c mice were immunized by injecting them subcutaneously with 200 ug of the chlorpyrifos-thyroglobulin immunogen in Freund's complete adjuvant. Three 100 ug subcutaneous injections with Freund's incomplete adjuvant followed, 3, 5 and 6 weeks after initial immunization. Three weeks after the last injection, a 100 ug i.p. injection was given in phosphate buffer saline. Mice were sacrificed, spleens were removed and their cells fused with PEG (polyethylene glycol) to P3X myeloma cells. Following screening and recloning, appropriate hybridomas were grown as ascites tumors in mice.
- PEG polyethylene glycol
- step (3) Added 500 uL of magnetic particles coupled to anti-chlorpyrifos antibodies to each tube and vortexed the test tube of step (2).
- B/Bo o is the absorbance at 450 nm observed for chlorpyrifos or a standardizing compound at that concentration divided by the absorbance at 450 nm observed with zero standard (just "Diluent Standard Buffer" buffer).
- FIGS. 2, 3, 4, and 5 show the value of B/Bo as a function of how long the chlorpyrifos was stored prior to testing in the assay.
- the temperature of storage either 50, 37, RT (room temperature), 4, or minus 20° C.
- the temperature of storage is shown at the right end of each line describing the stability.
- the concentrations of the various compounds were:
- Example 1 is performed except that instead of chlorpyrifos, samples with an unknown amount of chlorpyrifos is used.
- the results (B/Bo) are used to calculate the amount of chlorpyrifos in the sample for those samples that have chlorpyrifos in the chlorpyrifos concentration range tested in FIG. 1.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
Claims (39)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/303,343 US5576187A (en) | 1993-09-28 | 1994-09-09 | Standards for phosphorothioate insecticide immunoassays |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US12758393A | 1993-09-28 | 1993-09-28 | |
US08/303,343 US5576187A (en) | 1993-09-28 | 1994-09-09 | Standards for phosphorothioate insecticide immunoassays |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US12758393A Continuation-In-Part | 1993-09-28 | 1993-09-28 |
Publications (1)
Publication Number | Publication Date |
---|---|
US5576187A true US5576187A (en) | 1996-11-19 |
Family
ID=22430847
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US08/303,343 Expired - Fee Related US5576187A (en) | 1993-09-28 | 1994-09-09 | Standards for phosphorothioate insecticide immunoassays |
Country Status (3)
Country | Link |
---|---|
US (1) | US5576187A (en) |
AU (1) | AU7722294A (en) |
WO (1) | WO1995009364A1 (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100423526B1 (en) * | 2001-12-28 | 2004-03-18 | 이용태 | A Process for Preparing Haptens for Immunoassay of Phosphorothioate Pesticides |
KR100644205B1 (en) | 2005-06-30 | 2006-11-10 | 이용태 | Haptens and antibodies for the class-specific determination of organophosphorus pesticides and the immunoassay method using the same |
CN104262486A (en) * | 2014-09-24 | 2015-01-07 | 江苏省农业科学院 | Hybridoma cell strain FQ-2G3, monoclonal antibody produced by hybridoma cell strain FQ-2G3 and capable of preventing chlorpyrifos-methyl and immunochromatographic test strip |
CN107064494A (en) * | 2017-04-17 | 2017-08-18 | 四川精卫食品检测科技有限公司 | A kind of chlopyrifos detects enzyme linked immunological kit |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9212155B2 (en) | 2008-03-19 | 2015-12-15 | Aurimmed Pharma, Inc. | Compounds advantageous in the treatment of central nervous system diseases and disorders |
US10793515B2 (en) | 2008-03-19 | 2020-10-06 | Aurimmed Pharma, Inc. | Compounds advantageous in the treatment of central nervous system diseases and disorders |
US9206143B2 (en) | 2008-03-19 | 2015-12-08 | Aurimmed Pharma, Inc. | Compounds advantageous in the treatment of central nervous system diseases and disorders |
CN104267179B (en) * | 2014-09-25 | 2016-09-07 | 句容市农业技术推广中心 | A kind of isochlorothion colloidal gold fast detecting test paper strip and preparation method thereof |
CN106290830A (en) * | 2016-07-26 | 2017-01-04 | 大连民族大学 | A kind of based on up-conversion fluorescence nanoparticle quickly immune chromatography test paper detecting fenifrothion and preparation method thereof |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1991000294A1 (en) * | 1989-06-30 | 1991-01-10 | Commonwealth Scientific And Industrial Research Organisation | Monoclonal and polyclonal antibodies and test method for determination of fenitrothion and closely related organophosphates |
-
1994
- 1994-09-09 AU AU77222/94A patent/AU7722294A/en not_active Abandoned
- 1994-09-09 WO PCT/US1994/010070 patent/WO1995009364A1/en active Application Filing
- 1994-09-09 US US08/303,343 patent/US5576187A/en not_active Expired - Fee Related
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1991000294A1 (en) * | 1989-06-30 | 1991-01-10 | Commonwealth Scientific And Industrial Research Organisation | Monoclonal and polyclonal antibodies and test method for determination of fenitrothion and closely related organophosphates |
Non-Patent Citations (12)
Title |
---|
A. Hill et al., Issued 1992, Journal of Agricultural and Food Chemistry, "Mono- and Polychlonal Antibodies to the Organophosphate Fenitrothion. 2. Antibody Specificity and Assay Performance," vol. 40, pp. 1471-1474. |
A. Hill et al., Issued 1992, Journal of Agricultural and Food Chemistry, Mono and Polychlonal Antibodies to the Organophosphate Fenitrothion. 2. Antibody Specificity and Assay Performance, vol. 40, pp. 1471 1474. * |
D. McAdam et al., Issued 1992, Journal of Agricultural and Food Chemistry, "Mono- and Polyclonal Antibodies to the Organophosphate Fenitrothion. 1. Approaches to Hapten-Protein Conjugation," vol. 40, pp. 1466-1470. |
D. McAdam et al., Issued 1992, Journal of Agricultural and Food Chemistry, Mono and Polyclonal Antibodies to the Organophosphate Fenitrothion. 1. Approaches to Hapten Protein Conjugation, vol. 40, pp. 1466 1470. * |
H. Dahn et al., Magnetic Resonance in Chemistry, "NMR of Terminal Oxygen 9*--17 O NMR of the P--O `Double Bond`: Phosphine Oxides, Phosphinates, Phosphonates, Acylphosphonates and Related Compounds,", vol. 30, pp. 1089-1096. |
H. Dahn et al., Magnetic Resonance in Chemistry, NMR of Terminal Oxygen 9* 17 O NMR of the P O Double Bond : Phosphine Oxides, Phosphinates, Phosphonates, Acylphosphonates and Related Compounds, , vol. 30, pp. 1089 1096. * |
J. Skerritt et al., Issued 1992, Journal of AOAC International, "Enzyme-Linked Immunosorbent Assay for Quantitation of Organophosphate Pesticides: Fenitrothion, Chlorpyrifos-methyl, and Pirimiphos-methyl in Wheat Grain and Flour-Milling Fractions," vol. 75, pp. 519-528. |
J. Skerritt et al., Issued 1992, Journal of AOAC International, Enzyme Linked Immunosorbent Assay for Quantitation of Organophosphate Pesticides: Fenitrothion, Chlorpyrifos methyl, and Pirimiphos methyl in Wheat Grain and Flour Milling Fractions, vol. 75, pp. 519 528. * |
J. Skerritt et al., Published 1991, American Chemical Society, ACS Symposium Series, "Testing Cereal Products and Samples by Immunoassay," Chapter 11, pp. 124-138. |
J. Skerritt et al., Published 1991, American Chemical Society, ACS Symposium Series, Testing Cereal Products and Samples by Immunoassay, Chapter 11, pp. 124 138. * |
S u di et al., Issued 1988, Kieler Milchwirtschaftliche Forschungsberichte, Studies on the development of an immuno assay for the group specific detection of the diethyl ester of phosphates, thiophosphates, dithiophosphates and phosphonates, vol. 40, pp. 179 203. * |
S udi et al., Issued 1988, Kieler Milchwirtschaftliche Forschungsberichte, "Studies on the development of an immuno assay for the group-specific detection of the diethyl ester of phosphates, thiophosphates, dithiophosphates and phosphonates," vol. 40, pp. 179-203. |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100423526B1 (en) * | 2001-12-28 | 2004-03-18 | 이용태 | A Process for Preparing Haptens for Immunoassay of Phosphorothioate Pesticides |
KR100644205B1 (en) | 2005-06-30 | 2006-11-10 | 이용태 | Haptens and antibodies for the class-specific determination of organophosphorus pesticides and the immunoassay method using the same |
CN104262486A (en) * | 2014-09-24 | 2015-01-07 | 江苏省农业科学院 | Hybridoma cell strain FQ-2G3, monoclonal antibody produced by hybridoma cell strain FQ-2G3 and capable of preventing chlorpyrifos-methyl and immunochromatographic test strip |
CN107064494A (en) * | 2017-04-17 | 2017-08-18 | 四川精卫食品检测科技有限公司 | A kind of chlopyrifos detects enzyme linked immunological kit |
CN107064494B (en) * | 2017-04-17 | 2019-12-24 | 四川精卫食品检测科技有限公司 | Enzyme linked immunosorbent assay kit for chlorpyrifos detection |
Also Published As
Publication number | Publication date |
---|---|
WO1995009364A1 (en) | 1995-04-06 |
AU7722294A (en) | 1995-04-18 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP1019724B1 (en) | Diagnostic tests and kits for clostridium difficile | |
US5576187A (en) | Standards for phosphorothioate insecticide immunoassays | |
EP0139389A1 (en) | Antiidiotypic monoclonal antibody reagents and immunoassays employing antiidiotypic monoclonal antibody reagents | |
WO1998045684A9 (en) | Methods for concentrating ligands using magnetic particles | |
EP0767914A1 (en) | Antibodies to lipoproteins and apolipoproteins and methods of use thereof | |
US5185264A (en) | Diluent buffer and method for diluting an assay component | |
EP0699305B1 (en) | Methods, agents and kits for detection of organophosphorus compounds | |
Brustolin et al. | Immunoturbidimetric method for routine determinations of apolipoproteins AI and B | |
US4692330A (en) | Process for accelerating antigen-antibody reaction | |
AU751938B2 (en) | Immunoassay reagents and immunoassay method | |
EP0479834B1 (en) | Competitive enzyme assay for determination of phosphothioate insecticides | |
EP1233269B1 (en) | Agent for the removal of turbidity in biological samples | |
Barton et al. | Kinetic and immunochemical studies of a receptor-like protein that binds aromatic hydrocarbons. | |
US20040053426A1 (en) | Method of immunity examination with insoluble carrier particle and reagent therefor | |
El Guindi et al. | An immunoassay for human transferrin | |
US9541550B2 (en) | Method for immunologically measuring soluble LR11 | |
US5017472A (en) | Microflotation devices used for immunoassays and cell/molecular fractionation | |
WO2001088547A2 (en) | Standardized oxidized ldl assay | |
US5397699A (en) | Method for stabilizing an alkanol amine buffer used in optical determinations of enzyme activity | |
AU646738B2 (en) | Denatured vehicular proteins to improve enzyme linked immunosorbent assays | |
Eremin et al. | Immunochemical methods for the assays of herbicides of the 1, 3, 5-triazine group | |
JP2001330616A (en) | Method for manufacturing antigen-binding solid phase | |
CN104090097B (en) | Aqueous solution of specificity binding interaction culture medium serving as binding pair | |
US5780250A (en) | Immunoassay standards for polyaromatic hydrocarbon detection | |
Robison | Use of commercially available ELISA kits for detection of foodborne pathogens |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AS | Assignment |
Owner name: DOWELANCO, INDIANA Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:RUBIO, FERNANDO M.;LAWRUK, TIMOTHY S.;REEL/FRAME:007814/0101 Effective date: 19950202 Owner name: OHMICRON TECHNOLOGY, INC., DELAWARE Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:RUBIO, FERNANDO M.;LAWRUK, TIMOTHY S.;REEL/FRAME:007814/0101 Effective date: 19950202 |
|
FEPP | Fee payment procedure |
Free format text: PAYOR NUMBER ASSIGNED (ORIGINAL EVENT CODE: ASPN); ENTITY STATUS OF PATENT OWNER: LARGE ENTITY |
|
FEPP | Fee payment procedure |
Free format text: PAYOR NUMBER ASSIGNED (ORIGINAL EVENT CODE: ASPN); ENTITY STATUS OF PATENT OWNER: LARGE ENTITY Free format text: PAYER NUMBER DE-ASSIGNED (ORIGINAL EVENT CODE: RMPN); ENTITY STATUS OF PATENT OWNER: LARGE ENTITY |
|
FPAY | Fee payment |
Year of fee payment: 4 |
|
AS | Assignment |
Owner name: PNC BANK DELAWARE, DELAWARE Free format text: SECURITY AGREEMENT;ASSIGNOR:STRATEGIC DIANGNOSTICS INC.;REEL/FRAME:010832/0397 Effective date: 20000505 |
|
REMI | Maintenance fee reminder mailed | ||
LAPS | Lapse for failure to pay maintenance fees | ||
STCH | Information on status: patent discontinuation |
Free format text: PATENT EXPIRED DUE TO NONPAYMENT OF MAINTENANCE FEES UNDER 37 CFR 1.362 |
|
FP | Lapsed due to failure to pay maintenance fee |
Effective date: 20041119 |
|
AS | Assignment |
Owner name: STRATEGIC DIAGNOSTICS INC., DELAWARE Free format text: RELEASE BY SECURED PARTY;ASSIGNOR:PNC BANK;REEL/FRAME:027317/0171 Effective date: 20111202 |