US5281393A - Multilayered device containing a polymer-copper complex to control interference of biological substances in colorimetric test systems - Google Patents
Multilayered device containing a polymer-copper complex to control interference of biological substances in colorimetric test systems Download PDFInfo
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- US5281393A US5281393A US08/015,445 US1544593A US5281393A US 5281393 A US5281393 A US 5281393A US 1544593 A US1544593 A US 1544593A US 5281393 A US5281393 A US 5281393A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
- G01N33/728—Bilirubin; including biliverdin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
- G01N33/721—Haemoglobin
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/145555—Hetero-N
- Y10T436/146666—Bile pigment
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25125—Digestion or removing interfering materials
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
- Y10T436/255—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction
Definitions
- red blood cells In the analysis of blood for analytes such as glucose, lactate, uric acid and ethanol it is common practice to first of all reduce the whole blood sample being tested to plasma by precipitating red blood cells or to blood serum by causing the blood to coagulate. In either case, in the absence of extreme care in preparing the sample, a portion of the red blood cells can be lysed thereby releasing biological impurities such as hemoglobin and bilirubin derived from hemoglobin into the blood sample.
- an oxidative reagent system in which an oxidase enzyme selected in accordance with a predetermined analyte such as glucose reacts with the analyte in the presence of oxygen to provide hydrogen peroxide which, in the presence of a peroxidative substance, will oxidize a suitable redox indicator to convert it to its oxidized, colored form and, by visually or instrumentally determining the intensity of the color one can quantitatively determine the concentration of analyte in the blood sample.
- a predetermined analyte such as glucose reacts with the analyte in the presence of oxygen to provide hydrogen peroxide which, in the presence of a peroxidative substance, will oxidize a suitable redox indicator to convert it to its oxidized, colored form and, by visually or instrumentally determining the intensity of the color one can quantitatively determine the concentration of analyte in the blood sample.
- the present invention can be used in combination with reductive system which is represented by the following equations in which glucose is the analyte of interest, ATP represents adenosine triphosphae, ADP represents adenosine diphosphate, NAD represents nicotinamide-adenine dinucleotide, NADH represents the reduced form of NAD, and NBT represents nitroblue tetrazolium: ##STR1##
- the intensity of the formazan color produced by this system is directly proportional to the glucose concentration in the system.
- the present invention is an improvement in the method of analyzing blood serum or plasma by a colorimetric test in which a redox indicator is reduced or oxidized by the use of an appropriate reagent system to provide a colored response to the analyte.
- the improvement involves contacting the blood sample with an insoluble copper complex of a polymeric material bearing multiple pendant carboxyl groups to form an insoluble reaction product with potentially interferring biological substances in the blood sample which can be removed therefrom by solid/liquid separatory techniques.
- the present invention is directed to a method for the removal of colored interferants from blood serum or plasma.
- the method involves the ability of Cu ++ to complex with polymers containing multiple pendant carboxyl groups which complex in turn has the ability to form an insoluble combination with biological materials such as hemoglobin and bilirubin present in the blood sample thereby facilitating their removal therefrom.
- the carboxylic group containing polymers useful in the present invention are generally water soluble in their uncomplexed state, however, when they are converted to their copper complex the resultant is essentially insoluble in water.
- Suitable carboxylic group polymers include those polymers which are capable of forming water insoluble complexes with copper-ions when they are contacted in aqueous solution with a soluble copper salt, e.g. cupric sulfate.
- the carboxyl group density i.e. ratio of carboxyl groups to the number of carbon atoms in the polymer backbone, is not critical and will typically be within the range of from 1:9 to 9:1 and preferably within the range of from 1:3 to 3:1.
- n 2 0 to 3
- n 3 0 to 3, provided that at least 1 of n 1 , n 2 or n 3 is not 0;
- n 4 , n 5 , n 6 , n 7 and n 8 0 to 6;
- R 1 , R 2 , R 3 , R 4 and R 5 are H, OH, alkoxy containing from 1 to 4 carbon atoms, ##STR3## or --NHR 11 where R 11 is lower alkyl of 1 to 6 carbon atoms.
- R 6 , R 7 , R 8 , R 9 and R 10 are H, carboxyl, OH, sulfonic acid, imidazolyl or pyridyl, provided that at least 1 of R 6 , R 7 , R 8 , R 9 and R 10 , and preferably 2 or 3 of these moieties represent carboxyl groups, and X 1 , X 2 , X 3 are a single bond, --O--, --NR 12 , ##STR4## where R 12 is H or lower alkyl of 1 to 6 carbon atoms.
- the degree of polymerization i.e. value of m in the above formula, is not critical provided that it is within a range that forms a polymer copper complex when contacted with copper ions in aqueous solution. Typically, the degree of polymerization will be from about 10 to about 600 with a degree of polymerization of from about 40 to about 130 being preferred.
- the sulfonic acid, imidazolyl and pyridyl groups when present, enhance the solubility characteristics of the polymer in aqueous solution before its complexation with copper. Such enhanced solubility may be necessary when a polymer having a carboxyl group density towards the low end of the density ranges mentioned above is used to prepare the complex.
- carboxylic derivative polymers such as maleic anhydride/styrene copolymers can be used provided that they provide the requisite ratio of carboxylate to carbon atoms in the polymer backbone.
- preferred polymers include: ##STR5##
- the most preferred polycarboxylic polymer is the hydrolyzed form of Gantrez®, poly(methylvinyl ether/maleic acid) which is manufactured by the GAF Corporation, ##STR6##
- the carboxylic acid polymer is complex with copper by contacting it in aqueous solution with a suitable source of copper ion, e.g. copper sulfate. In this procedure the polymer/copper complex precipitates from the aqueous medium and is recovered by filtration or similar means.
- a suitable source of copper ion e.g. copper sulfate.
- the soluble polymer bearing multiple carboxyl groups is rendered essentially insoluble in water upon being complexed with copper which makes it an ideal candidate for scavenging biological materials from blood serum or plasma because the combination of polymer-copper complex/biological material can be readily separated from serum or plasma by standard solid/liquid separatory techniques such as centrifugation with decantation or by filtration.
- the polymer-copper complex is ingrained in a filter means, such as filter paper, and the serum or plasma is passed through the filter paper wherein the biological materials, particularly hemoglobin and bilirubin, adhere to the filter bound complex and are effectively removed from the blood sample.
- a filter means such as filter paper
- the biological materials particularly hemoglobin and bilirubin
- the complex is added directly to the blood sample also works well.
- the insoluble reaction product of the polymer-copper complex and biological material can be separated from the blood sample by centrifugation with decantation or by filtration.
- the polymer-copper complex will be combined with the blood sample in an amount of from about 0.2 to about 20% w/v with the preferred amount being from about 0.5% to about 10%.
- This concentration will vary, of course, depending on the amount of biological material to be scavenged and the concentration of carboxyl groups on the polymer backbone which are capable of being derivatized with copper.
- the preferred copper complex, prepared using Gantrez®, will normally be combined with the blood serum or plasma in an amount of from about 0.7 to about 7% w/v in order to achieve the desired level of purification.
- hemoglobin and bilirubin are bound by interaction of their carboxylic acid and/or amino groups with the previously described polymer/copper complex.
- a Gantrez® acid/Cu ++ complex was prepared as follows:
- Gantrez® S-95 is the hydrolyzed form of Gantrez®, poly(methyl vinyl ether/maleic anhydride).
- cupric sulfate 0.1M was added to the Gantrez® acid solution slowly with vigorous stirring. After mixing for 30 minutes a blue precipitate separated out of the solution. This precipitate was collected by filtration and washed with deionized water thoroughly until the filtrate was colorless in order to remove all of the unbound cupric ion. This solid was dried to provide a blue Gantrez® acid copper complex.
- Serum test tubes containing 0, 10, 22.8, 35.7, 46.5, 60.8, 71.8 and 84 mg Gantrez® acid-copper complex were mixed with 10 ⁇ g of conjugated bilirubin and distilled water up to 1.3 mL. Each tube was shaken for one minute and centrifuged. The supernatant solution was separated and an absorbance measurement was taken at 456 nm. The control sample contained bilirubin without any addition of the complex. The results of this experiment are set out in Table II.
- a Gantraz®-Acid copper complex was applied to an absorbant carrier matrix, i.e. filter paper.
- a piece of filter paper was first impregnated from its 0.25M solution of Gantrez® Acid. After drying at 50° C. for 10 minutes, the matrix was impregnated with a second solution containing 0.125M cupric sulfate and again dried at 50° C. for 10 minutes.
- the complex treated matrix was cut into 0.2 ⁇ 0.2 inch wide ribbons and arranged in a two-layer stacked format to provide a reagent pad.
- the matrix Gantrez®-Acid copper complex was contacted with aqueous hemoglobin at various concentrations by pipetting the hemoglobin solution onto the upper layer of the test pad.
- K/S value as determined from the equation: ##EQU1## to transform the reflectance data (R) to a function (K/S) that is proportional to the chromophore concentration.
- the lower pad contained no reagent so that the reflectance values represent only the amount of hemoglobin which penetrated the first upper pad and entered the lower pad.
- the lower pad would be impregnated with a reagent/enzyme system of the type previously described to provide a color change when contacted with a predetermined analyte in the blood sample.
- Table III demonstrate that the interference of hemoglobin with the colorimetric determination of the analyte is greatly diminished by using the system of the present invention.
- This experiment illustrates how a stacked device for the one step colormetric analysis for various analytes can be prepared.
- a device would comprise a reagent layer having a filter layer of absorbant material impregnated with the polymer-copper complex located above it through which the blood serum or plasma passes.
- This stacked system which may optionally have a filter layer on top of the complex containing layer for removing red blood cells, may be affixed to a transparent support such as a clear polyester strip through which the reaction of the blood with components in the reagent layer can be viewed with reduced interference from hemoglobin or bilirubin which has been effectively removed from the blood sample before it reaches the reagent layer.
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Abstract
A layered device suitable for use in the analysis of blood samples which includes a first layer of a matrix material having dispersed therein a complex of copper and a polymer containing multiple pendant carboxyl groups which selectively combines with hemoglobin and bilirubin in fluid communication with a second layer which contains a reagent system which reacts with one or more predetermined analytes in the blood sample to provide a color change.
Description
This is a division of application Ser. No. 07/816,339, filed on Dec. 30, 1991, and issued as U.S. Pat. No. 5,229,296, on Jul. 20, 1993.
There are available to the clinical analyst various test devices and methods for the rapid determination of certain constituents in blood samples which if found to be outside the range of a predetermined concentration indicate a potential pathological condition.
In the analysis of blood for analytes such as glucose, lactate, uric acid and ethanol it is common practice to first of all reduce the whole blood sample being tested to plasma by precipitating red blood cells or to blood serum by causing the blood to coagulate. In either case, in the absence of extreme care in preparing the sample, a portion of the red blood cells can be lysed thereby releasing biological impurities such as hemoglobin and bilirubin derived from hemoglobin into the blood sample.
In the analysis of the blood sample for a predetermined analyte, there is typically employed an oxidative reagent system in which an oxidase enzyme selected in accordance with a predetermined analyte such as glucose reacts with the analyte in the presence of oxygen to provide hydrogen peroxide which, in the presence of a peroxidative substance, will oxidize a suitable redox indicator to convert it to its oxidized, colored form and, by visually or instrumentally determining the intensity of the color one can quantitatively determine the concentration of analyte in the blood sample.
In addition to the oxidative system described above, the present invention can be used in combination with reductive system which is represented by the following equations in which glucose is the analyte of interest, ATP represents adenosine triphosphae, ADP represents adenosine diphosphate, NAD represents nicotinamide-adenine dinucleotide, NADH represents the reduced form of NAD, and NBT represents nitroblue tetrazolium: ##STR1## The intensity of the formazan color produced by this system is directly proportional to the glucose concentration in the system.
In order for such a test to have the required sensitivity, it is essential that the blood specimen being tested be as nearly colorless as is practical under the test conditions in order to avoid interference with the determination of the intensity of the color formed. Accordingly, lysis of the red blood cells and the consequent release of biological substances such as hemoglobin and bilirubin is problematical in the context of these colorimetric determinations due to their interference with the reading of the specimen's reflectance by spectrophotometric means.
In their article appearing in Cancer Res., 47(14), 3624-6 (1987) Anderson et al report that human serum alpha-fetoprotein and albumin were chromatographed on immobilized iminodiacetic acid charged with either Co+2, Ni+2, Cu+2 or Zn+2. Both proteins were bound to Cu+2 and were partially resolved by affinity elution with imidazole.
Berk et al report in Biomed. Appln. Immobilized Enzymes & Proteins, Vol. 1, Pp. 297-317 that bilirubin can be removed from blood by affinity-competition chromatography over an albumin-agarose gel.
It is an object of the present invention to provide a novel method for the removal of extraneous biological substances such as hemoglobin and/or bilirubin from samples of blood serum or plasma.
It is a further object to provide such a method which renders the blood sample more amenable to the colormetric analysis of various analytes contained therein.
The present invention is an improvement in the method of analyzing blood serum or plasma by a colorimetric test in which a redox indicator is reduced or oxidized by the use of an appropriate reagent system to provide a colored response to the analyte. The improvement involves contacting the blood sample with an insoluble copper complex of a polymeric material bearing multiple pendant carboxyl groups to form an insoluble reaction product with potentially interferring biological substances in the blood sample which can be removed therefrom by solid/liquid separatory techniques.
The present invention is directed to a method for the removal of colored interferants from blood serum or plasma. The method involves the ability of Cu++ to complex with polymers containing multiple pendant carboxyl groups which complex in turn has the ability to form an insoluble combination with biological materials such as hemoglobin and bilirubin present in the blood sample thereby facilitating their removal therefrom.
The carboxylic group containing polymers useful in the present invention are generally water soluble in their uncomplexed state, however, when they are converted to their copper complex the resultant is essentially insoluble in water. Suitable carboxylic group polymers include those polymers which are capable of forming water insoluble complexes with copper-ions when they are contacted in aqueous solution with a soluble copper salt, e.g. cupric sulfate. The carboxyl group density, i.e. ratio of carboxyl groups to the number of carbon atoms in the polymer backbone, is not critical and will typically be within the range of from 1:9 to 9:1 and preferably within the range of from 1:3 to 3:1. Ideally, this ratio will be about 1:1 which provides the best combination of solubility characteristics and affinity for the potentially interferring biological materials upon its conversion to the copper complex. Accordingly, suitable polymers can be selected from polymers of the general formula: ##STR2## where n1 =0 to 3
n2 =0 to 3
n3 =0 to 3, provided that at least 1 of n1, n2 or n3 is not 0;
n4, n5, n6, n7 and n8 =0 to 6;
R1, R2, R3, R4 and R5 are H, OH, alkoxy containing from 1 to 4 carbon atoms, ##STR3## or --NHR11 where R11 is lower alkyl of 1 to 6 carbon atoms. R6, R7, R8, R9 and R10 are H, carboxyl, OH, sulfonic acid, imidazolyl or pyridyl, provided that at least 1 of R6, R7, R8, R9 and R10, and preferably 2 or 3 of these moieties represent carboxyl groups, and X1, X2, X3 are a single bond, --O--, --NR12, ##STR4## where R12 is H or lower alkyl of 1 to 6 carbon atoms.
The degree of polymerization, i.e. value of m in the above formula, is not critical provided that it is within a range that forms a polymer copper complex when contacted with copper ions in aqueous solution. Typically, the degree of polymerization will be from about 10 to about 600 with a degree of polymerization of from about 40 to about 130 being preferred.
The sulfonic acid, imidazolyl and pyridyl groups, when present, enhance the solubility characteristics of the polymer in aqueous solution before its complexation with copper. Such enhanced solubility may be necessary when a polymer having a carboxyl group density towards the low end of the density ranges mentioned above is used to prepare the complex. In addition, carboxylic derivative polymers such as maleic anhydride/styrene copolymers can be used provided that they provide the requisite ratio of carboxylate to carbon atoms in the polymer backbone.
More specifically, preferred polymers include: ##STR5##
The most preferred polycarboxylic polymer is the hydrolyzed form of Gantrez®, poly(methylvinyl ether/maleic acid) which is manufactured by the GAF Corporation, ##STR6##
The carboxylic acid polymer is complex with copper by contacting it in aqueous solution with a suitable source of copper ion, e.g. copper sulfate. In this procedure the polymer/copper complex precipitates from the aqueous medium and is recovered by filtration or similar means.
The soluble polymer bearing multiple carboxyl groups is rendered essentially insoluble in water upon being complexed with copper which makes it an ideal candidate for scavenging biological materials from blood serum or plasma because the combination of polymer-copper complex/biological material can be readily separated from serum or plasma by standard solid/liquid separatory techniques such as centrifugation with decantation or by filtration. In a preferred method of practicing the present invention the polymer-copper complex is ingrained in a filter means, such as filter paper, and the serum or plasma is passed through the filter paper wherein the biological materials, particularly hemoglobin and bilirubin, adhere to the filter bound complex and are effectively removed from the blood sample. An alternative procedure in which the complex is added directly to the blood sample also works well. In this embodiment, the insoluble reaction product of the polymer-copper complex and biological material can be separated from the blood sample by centrifugation with decantation or by filtration.
Typically, the polymer-copper complex will be combined with the blood sample in an amount of from about 0.2 to about 20% w/v with the preferred amount being from about 0.5% to about 10%. This concentration will vary, of course, depending on the amount of biological material to be scavenged and the concentration of carboxyl groups on the polymer backbone which are capable of being derivatized with copper. The preferred copper complex, prepared using Gantrez®, will normally be combined with the blood serum or plasma in an amount of from about 0.7 to about 7% w/v in order to achieve the desired level of purification.
While we do not wish to be bound by any particular theory of how our invention operates, it is believed that hemoglobin and bilirubin are bound by interaction of their carboxylic acid and/or amino groups with the previously described polymer/copper complex.
The method of practicing the present invention is further illustrated by the following examples:
A Gantrez® acid/Cu++ complex was prepared as follows:
About 1.7 g (0.1M) of Gantrez® S-95 was mixed with 100 ml of deionized water until it was completely dissolved. Gantrez® S-95 is the hydrolyzed form of Gantrez®, poly(methyl vinyl ether/maleic anhydride). Next a solution of cupric sulfate (0.1M) was added to the Gantrez® acid solution slowly with vigorous stirring. After mixing for 30 minutes a blue precipitate separated out of the solution. This precipitate was collected by filtration and washed with deionized water thoroughly until the filtrate was colorless in order to remove all of the unbound cupric ion. This solid was dried to provide a blue Gantrez® acid copper complex.
The effectiveness of this complex in a liquid assay and in the solid phase to cause hemoglobin and bilirubin separation was determined by obtaining test results using both a liquid and filter paper format and comparing these test results with those obtained using the same formats without the complex.
Six test tubes containing 0, 10, 20, 30, 50 and 70 mg of the Gantrez® acid-copper complex were mixed with 780 μg hemoglobin and distilled water up to 1.3 mL. Each tube was shaken for one minute and then centrifuged. The supernatant solution was separated and an absorbance measurement was made using a Beckman spectrophotometer at 560 nm (the wavelength of maximum absorbance for hemoglobin). A blank sample containing no complex was used as control. The results of this experiment are set out in Table I.
TABLE I ______________________________________ Complex ABS at 560 nm % Reduction ______________________________________ None 0.2929 -- 10 mg 0.1680 43.00 20 mg 0.1377 53.00 30 mg 0.1282 56.00 50 mg 0.0891 69.59 70 mg 0.0640 78.50 ______________________________________
Serum test tubes containing 0, 10, 22.8, 35.7, 46.5, 60.8, 71.8 and 84 mg Gantrez® acid-copper complex were mixed with 10 μg of conjugated bilirubin and distilled water up to 1.3 mL. Each tube was shaken for one minute and centrifuged. The supernatant solution was separated and an absorbance measurement was taken at 456 nm. The control sample contained bilirubin without any addition of the complex. The results of this experiment are set out in Table II.
TABLE II ______________________________________ Complex ABS at 456 nm % Reduction ______________________________________ Control 0.6198 -- 10 mg 0.3336 46.00 22.8 mg 0.3491 44.00 35.7 mg 0.3502 44.00 46.5 mg 0.3087 50.00 60.8 mg 0.2978 52.00 71.8 mg 0.2967 52.00 84.0 mg 0.2287 55.00 ______________________________________
From Tables I and II it can be determined that the polymer/Cu complex is effective in removing both hemoglobin and bilirubin from aqueous media.
In this experiment a Gantraz®-Acid copper complex was applied to an absorbant carrier matrix, i.e. filter paper. A piece of filter paper was first impregnated from its 0.25M solution of Gantrez® Acid. After drying at 50° C. for 10 minutes, the matrix was impregnated with a second solution containing 0.125M cupric sulfate and again dried at 50° C. for 10 minutes. The complex treated matrix was cut into 0.2×0.2 inch wide ribbons and arranged in a two-layer stacked format to provide a reagent pad. The matrix Gantrez®-Acid copper complex was contacted with aqueous hemoglobin at various concentrations by pipetting the hemoglobin solution onto the upper layer of the test pad.
The results of this experiment are set out in Table III.
TABLE III ______________________________________ Hemoglobin K/S K/S Conc. Hemoglobin Hemoglobin + mg/dL Samples Complex % Reduction ______________________________________ 0 0.0753 0.0753 -- 10 0.1117 0.0812 84.00 250 0.1485 0.0861 85.25 500 0.2529 0.1380 64.70 1000 0.4153 0.2639 44.53 2000 0.7826 0.6546 18.10 ______________________________________
where K/S value as determined from the equation: ##EQU1## to transform the reflectance data (R) to a function (K/S) that is proportional to the chromophore concentration.
For purposes of this example, the lower pad contained no reagent so that the reflectance values represent only the amount of hemoglobin which penetrated the first upper pad and entered the lower pad. In practice, the lower pad would be impregnated with a reagent/enzyme system of the type previously described to provide a color change when contacted with a predetermined analyte in the blood sample. The data of Table III demonstrate that the interference of hemoglobin with the colorimetric determination of the analyte is greatly diminished by using the system of the present invention.
This experiment illustrates how a stacked device for the one step colormetric analysis for various analytes can be prepared. Such a device would comprise a reagent layer having a filter layer of absorbant material impregnated with the polymer-copper complex located above it through which the blood serum or plasma passes. This stacked system, which may optionally have a filter layer on top of the complex containing layer for removing red blood cells, may be affixed to a transparent support such as a clear polyester strip through which the reaction of the blood with components in the reagent layer can be viewed with reduced interference from hemoglobin or bilirubin which has been effectively removed from the blood sample before it reaches the reagent layer.
Claims (14)
1. A device suitable for use in the analysis of blood samples which device comprises:
a) a first upper layer of an absorbent matrix having dispersed therein a water insoluble complex of copper and a polymer containing multiple pendant carboxyl groups, which complex selectively combines with hemoglobin and bilirubin; and
b) a second lower layer, in fluid communication with said first layer, said second layer comprising an absorbent matrix having dispersed therein a reagent system which reacts with predetermined analytes other than hemoglobin and bilirubin contained in a blood sample applied to the device to provide a color change which indicates the presence or amount of said predetermined analytes in the blood sample.
2. The device of claim 1 wherein the polymer is polyacrylic acid, polyaspartic acid, polyglutamic acid or poly(methyl vinyl ether/maleic acid).
3. A method of determining the presence or concentration of one or more analytes in a blood sample which comprises applying a blood sample to the upper layer of the device of claim 1 and allowing the blood sample to penetrate said upper layer and flow into said lower layer to cause the reagent system to undergo a color change which can be detected by visual or instrumental means.
4. The device of claim 1 wherein the polymer is characterized by the formula: ##STR7## where n1 =0 to 3
n2 =0 to 3
n3 =0 to 3, provided that at least 1 of n1, n2 or n3 is not 0;
n4, n5, n6, n7 and n8 independently=0 to 6;
X1, X2 and X3 represent a single bond, --O--, --NR12, ##STR8## where R12 is H or lower alkyl of 1 to 6 carbon atoms; R1, R2, R3, R4 and R5 are independently H, OH, alkoxy of 1 to 4 carbon atoms, ##STR9## or --NHR11 where R11 is lower alkyl of 1 to 6 carbon atoms and R6, R7, R8, R9 and R10 are independently H, carboxyl, OH, sulfonic acid, imidazolyl or pyridyl;
provided that at least 1 of R6, R7, R8, R9 and R10 is a carboxyl group and m is the degree of polymerization.
5. The device of claim 4 wherein two or more of R6, R7, R8, R9 or R10 represent carboxyl.
6. The device of claim 5 wherein the ratio of carboxyl groups to the number of carbon atoms in the polymer backbone is in the range of 1:9 to 9:1.
7. The device of claim 6 wherein the ratio is in the range of 1:3 to 3:1.
8. The device of claim 7 wherein the ratio is about 1:1.
9. The device of claim 8 wherein m can range from about 10 to about 600.
10. The device of claim 9 wherein m can range from about 40 to about 130.
11. The device of claim 1 wherein the polymer is hydrolyzed poly(methylvinyl ether/maleic acid).
12. The device of claim 1 wherein the complex is present in the absorbant matrix of the upper layer in an amount of from about 0.2 to about 20% w/v of a blood sample applied to the device.
13. The device of claim 12 wherein the complex is present in an amount of from about 0.5 to about 10% w/v.
14. The device of claim 1 wherein the polymer is hydrolyzed poly(methylvinyl ether/maleic acid) and its copper complex is present in the absorbent matrix of the upper layer in an amount of from about 0.7 to about 10% w/v of a blood sample applied to the device.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/015,445 US5281393A (en) | 1991-12-30 | 1993-02-09 | Multilayered device containing a polymer-copper complex to control interference of biological substances in colorimetric test systems |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/816,339 US5229296A (en) | 1991-12-30 | 1991-12-30 | Use of polymer-copper complex to control interference of biological substances in colorimetric test systems |
US08/015,445 US5281393A (en) | 1991-12-30 | 1993-02-09 | Multilayered device containing a polymer-copper complex to control interference of biological substances in colorimetric test systems |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
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US07/816,339 Division US5229296A (en) | 1991-12-30 | 1991-12-30 | Use of polymer-copper complex to control interference of biological substances in colorimetric test systems |
Publications (1)
Publication Number | Publication Date |
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US5281393A true US5281393A (en) | 1994-01-25 |
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Application Number | Title | Priority Date | Filing Date |
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US07/816,339 Expired - Lifetime US5229296A (en) | 1991-12-30 | 1991-12-30 | Use of polymer-copper complex to control interference of biological substances in colorimetric test systems |
US08/015,445 Expired - Fee Related US5281393A (en) | 1991-12-30 | 1993-02-09 | Multilayered device containing a polymer-copper complex to control interference of biological substances in colorimetric test systems |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
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US07/816,339 Expired - Lifetime US5229296A (en) | 1991-12-30 | 1991-12-30 | Use of polymer-copper complex to control interference of biological substances in colorimetric test systems |
Country Status (5)
Country | Link |
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US (2) | US5229296A (en) |
EP (1) | EP0549970A1 (en) |
JP (1) | JP3256305B2 (en) |
AU (1) | AU655509B2 (en) |
CA (1) | CA2083445A1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5606116A (en) * | 1994-10-29 | 1997-02-25 | Kabushiki Kaisha Nippon Gene | Chromatogenous testing system for urinalysis |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
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US9759651B2 (en) | 2014-12-23 | 2017-09-12 | Magellan Diagnostics, Inc. | Combination optical hemoglobin and electrochemical lead assay |
US20170192021A1 (en) * | 2015-12-30 | 2017-07-06 | Magellan Diagnostics, Inc. | Optical bilirubin sensor and assay |
Citations (5)
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US4303408A (en) * | 1980-02-05 | 1981-12-01 | Eastman Kodak Company | Removal of interferents in analytical assays in a two phase interferent-removal zone |
US5057435A (en) * | 1990-10-12 | 1991-10-15 | Synermed, Inc. | Reagent and methods for calcium determination |
US5079140A (en) * | 1989-04-13 | 1992-01-07 | Miles Inc. | Agent for reducing ascorbic acid interference in liquid or dry phase assay systems and method relating thereto |
US5087421A (en) * | 1988-05-20 | 1992-02-11 | Konica Corporation | Multilayered assay element |
US5183762A (en) * | 1989-09-21 | 1993-02-02 | Diesse Diagnostica Senese S.R.L. | Copper containing reagent for the detection and determination of bilirubin in the urine |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NL126573C (en) * | 1965-08-02 | |||
US4600689A (en) * | 1983-11-21 | 1986-07-15 | Takara Shuzo Co., Ltd. | Novel bilirubin oxidase, its production and use |
JPS61108400A (en) * | 1984-10-31 | 1986-05-27 | Amano Pharmaceut Co Ltd | Determination of cholesterol |
-
1991
- 1991-12-30 US US07/816,339 patent/US5229296A/en not_active Expired - Lifetime
-
1992
- 1992-11-04 AU AU28144/92A patent/AU655509B2/en not_active Ceased
- 1992-11-20 CA CA002083445A patent/CA2083445A1/en not_active Abandoned
- 1992-12-17 EP EP92121474A patent/EP0549970A1/en not_active Withdrawn
- 1992-12-21 JP JP35538192A patent/JP3256305B2/en not_active Expired - Fee Related
-
1993
- 1993-02-09 US US08/015,445 patent/US5281393A/en not_active Expired - Fee Related
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4303408A (en) * | 1980-02-05 | 1981-12-01 | Eastman Kodak Company | Removal of interferents in analytical assays in a two phase interferent-removal zone |
US5087421A (en) * | 1988-05-20 | 1992-02-11 | Konica Corporation | Multilayered assay element |
US5079140A (en) * | 1989-04-13 | 1992-01-07 | Miles Inc. | Agent for reducing ascorbic acid interference in liquid or dry phase assay systems and method relating thereto |
US5183762A (en) * | 1989-09-21 | 1993-02-02 | Diesse Diagnostica Senese S.R.L. | Copper containing reagent for the detection and determination of bilirubin in the urine |
US5057435A (en) * | 1990-10-12 | 1991-10-15 | Synermed, Inc. | Reagent and methods for calcium determination |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5606116A (en) * | 1994-10-29 | 1997-02-25 | Kabushiki Kaisha Nippon Gene | Chromatogenous testing system for urinalysis |
Also Published As
Publication number | Publication date |
---|---|
EP0549970A1 (en) | 1993-07-07 |
US5229296A (en) | 1993-07-20 |
AU655509B2 (en) | 1994-12-22 |
JPH05249106A (en) | 1993-09-28 |
JP3256305B2 (en) | 2002-02-12 |
CA2083445A1 (en) | 1993-07-01 |
AU2814492A (en) | 1993-07-01 |
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