US3839183A - Electrophoresis sample applicator - Google Patents

Electrophoresis sample applicator Download PDF

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Publication number
US3839183A
US3839183A US00370348A US37034873A US3839183A US 3839183 A US3839183 A US 3839183A US 00370348 A US00370348 A US 00370348A US 37034873 A US37034873 A US 37034873A US 3839183 A US3839183 A US 3839183A
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Prior art keywords
applicator
arm
sample
strips
fingers
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Expired - Lifetime
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US00370348A
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G Klein
R Meyer
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Beckman Coulter Inc
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Beckman Instruments Inc
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Application filed by Beckman Instruments Inc filed Critical Beckman Instruments Inc
Priority to US00370348A priority Critical patent/US3839183A/en
Priority to CA199,521A priority patent/CA1005256A/en
Priority to GB2237474A priority patent/GB1446125A/en
Priority to IT23155/74A priority patent/IT1012799B/en
Priority to DK313674A priority patent/DK313674A/da
Priority to JP1974068010U priority patent/JPS5434635Y2/ja
Priority to SE7407911A priority patent/SE7407911L/xx
Priority to DE2428829A priority patent/DE2428829A1/en
Priority to CH817974A priority patent/CH580275A5/xx
Application granted granted Critical
Publication of US3839183A publication Critical patent/US3839183A/en
Priority to SE7810453A priority patent/SE7810453L/en
Anticipated expiration legal-status Critical
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44704Details; Accessories
    • G01N27/44743Introducing samples

Definitions

  • ABSTRACT This invention provides an apparatus and applicator for applying liquid sample to an electrophoretic membrane.
  • the applicator comprises two parallel strips of flexible plastic film separated by a spacer along one edge. At the other edge the strips form fingers between which are suspended liquid samples.
  • the appli cator is gripped at the laminated edge by the apparatus at the end of an arm pivotally mounted on a base plate.
  • the arm is connected by mechanical linkage to a handle which controls movement of the arm such that the applicator can be lowered to contact a membrane supported by the base plate with the correct amount of force to deposit the liquid samples cleanly and precisely on the membrane.
  • the gripping element of the apparatus can be adjusted to control the spacing between the fingers and thereby control the amount of sample deposited.
  • This invention provides an apparatus and applicator for applying liquid sample, such as blood serum, to a membrane, such as an agarose gel electrophoretic membrane.
  • the applicator is gripped by the apparatus at the end of an arm pivotally mounted on a base plate.
  • the arm is mechanically connected to a handle such that rotation of the handle results in rotation of the arm, whereby, when a membrane is placed on the base plate, rotation of the handle causes the applicator to contact the membrane and release sample contained by the applicator.
  • the means for gripping the applicator includes means for adjusting the horizontal alignment of the applicator and means for adjusting the sample holding capacity of the applicator.
  • the applicator comprises two strips of flexible film separated at one edge by a spacer laminated between the strips, with each strip having a plurality of fingers at the opposite edge which define capillary spaces between the strips for containing liquid sample.
  • FIG. 1 is a side view of the apparatus of this invention showing the arm in a slightly raised position with the applicator gripped at the end of the arm.
  • FIG. 2 is an exploded view of the apparatus of this invention.
  • FIG. 3 is a front view of an applicator used with the apparatus.
  • FIG. 4 is a side view of the applicator.
  • the apparatus comprises an arm pivotally mounted between two support blocks 11 secured to a base plate 12 by screws 13.
  • the arm 10 pivots on bushings 14 about pins 15.
  • Also pivotally mounted between the support blocks 11 is a handle 16, which pivots on bushings 17 about pins 18.
  • the handle 16 is connected to the arm 10 through a link 19.
  • the link 19 is secured to the handle 16 by pin 20 and to the arm 10 by pin 21 at places offset from the axes of rotation such that as the arm 10 approaches a horizontal position, a large angular movement of the handle 16 results in a cone spondingly smaller angular movement of the arm 10.
  • a jaw 23 contacts the face 22 and is held in place by an adjusting knob 24 which threadedly engages a screw 25 passing through the face 22.
  • the knob 24 has a spherical base which engages a spherical depression in the jaw 23.
  • the lower edge of the jaw 23 forms a lip 26.
  • a spring 27 biases the upper portion of the jaw 23 away from the face 22.
  • a fin extending from the jaw 23 engages a slot 28 in the face 22 and thereby limits the rotational freedom of the jaw 23.
  • the jaw 23 is free to rotate in a plane perpendicular to the surface of the face 22 about an axis passing through the knob 24.
  • the applicator comprises two strips 40 of flexible film, such as plastic (e.g., polyester) film, separated at the top by a spacer 41 laminated between the strips.
  • the inner surfaces of the strips are preferably coated with a hydrophilic material, such as gelatin.
  • the outer surfaces are preferably hydrophobic.
  • Each strip 40 has a plurality of fingers 42 which define capillary spaces between the strips for containing the liquid samples, which are released when the fingers contact the gel.
  • the fingers of one strip are preferably slightly longer than the fingers of the other strip so that the fingers cut and enter the gel cleanly. If the fingers were the same length, they would tend to crush the gel rather than pierce it.
  • the shorter fingers preferably have holes 43 in them to facilitate loading of sample.
  • the applicator is positioned between the jaw 23 and the face 22 by pushing the upper portion ofjaw 23 toward the face 22 to move the lip 26 away from the face, and then inserting the applicator such that a notch 44 in the top of the applicator engages a protrusion 29 on the face 22, followed by releasing the upper portion of jaw 23 to secure the applicator between the lip 26 and the face 22.
  • the protrusion 29 fixes the lateral position of the applicator while permitting it to rotate so that it can be aligned horizontally.
  • the applicator is aligned horizontally by lowering the arm 10 until the tips of the fingers 42 touch a slide (not shown) resting on the base plate 12. As the fingers 42 touch the slide, the grip on the applicator is relaxed briefly by squeezing the upper portion of the jaw 23 toward the face 22, which permits the tips of the fingers to align horizontally on the surface of the slide.
  • the spacing between the strips may be adjusted to control the amount of sample contained between the fingers.
  • the spacing is adjusted by rotating the adjusting knob 24.
  • the knob 24 When the knob 24 is rotated completely in one direction, the surface of the jaw 23 is parallel to the surface of the face 22.
  • the strips 40 of the applicator are then parallel and the space between them is equal to the thickness of the spacer 41.
  • the knob 24 When the knob 24 is rotated in the other direction, the jaw 23 becomes angular with respect to the face 22. Since the front fingers rest against the lip 26 of the jaw 23, while the back fingers rest against the surface of the face 22, as the jaw becomes angular the front fingers become bent toward the back fingers.
  • the lower edges of the strips 40 may be made closer or farther apart depending on the setting of the knob 24, with the result that a repeatable amount of sample can be deposited in the gel with a minimum of operator skill.
  • liquid sample is introduced into the space between the fingers of the adjacent strips.
  • a particularly convenient method of introducing sample is to dip the fingers into a trough divided into compartments corresponding to the fingers. Each compartment contains a separate sample for each pair of fingers. The trough is placed on the base plate 12 and the arm is lowered so that each finger enters its respective compartment of the trough. The samples flow into the spaces between the fingers by capillary action. Alternatively, sample may be introduced into the spaces by using a pipette.
  • the samples are deposited into a gel by placing the membrane on a slide resting on the base plate 12, and lowering the arm 10 by rotating the handle 16 until the fingers 42 contact the gel. As the fingers contact the gel, the sample is released.
  • the base plate 12 has two keypins for accurately positioning the slide by engaging holes in the bottom of the slide. As a result, the samples are automatically deposited precisely where desired in the gel. Slides with different keyhole patterns can be used for various application requirements. The keypins can also be used to position the sample trough.
  • the applicator illustrated herein is especially suitable for zone electrophoresis. However, other applicators,
  • Apparatus for applying liquid sample to a membrane comprising a base plate,
  • a handle connected by a mechanical linkage to the arm such that rotation of the handle results in rotation of the arm.
  • the gripping means includes means for adjusting the horizontal alignment of the applicator.
  • the gripping means includes means for adjusting the sample holding capacity of the applicator.
  • a jaw between the face and the knob the jaw being free to rotate in a plane perpendicular to the surface of the face about an axis passing through the adjusting knob.
  • a sample applicator comprising two substantially parallel strips of a flexible film separated at one edge by a spacer laminated between the strips, each strip having a plurality of fingers at the opposite edge which define capillary spaces between the strips for containing liquid sample.
  • each shorter finger has a hole in it to facilitate loading of sample.
  • the applicator of claim 13 having a notch at the laminated edge adapted to engage a protrusion for fixing lateral motion of the applicator.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

This invention provides an apparatus and applicator for applying liquid sample to an electrophoretic membrane. The applicator comprises two parallel strips of flexible plastic film separated by a spacer along one edge. At the other edge the strips form fingers between which are suspended liquid samples. The applicator is gripped at the laminated edge by the apparatus at the end of an arm pivotally mounted on a base plate. The arm is connected by mechanical linkage to a handle which controls movement of the arm such that the applicator can be lowered to contact a membrane supported by the base plate with the correct amount of force to deposit the liquid samples cleanly and precisely on the membrane. The gripping element of the apparatus can be adjusted to control the spacing between the fingers and thereby control the amount of sample deposited.

Description

United States Patent 1 Klein et al.
1 1 Oct. 1, 1974 1 i ELECTROPHORESIS SAMPLE APPLICATOR [75] Inventors: Gerald Lee Klein, Orange; Richard Carl Meyer, La Habra, both of Calif.
[73] Assignee: Beckman Instruments, Inc.,
Fullerton, Calif.
[22 Filed: June 15,1973
[21] Appl. No.: 370,348
[52] U.S. Cl. 204/299, 204/180 0, 204/180 5 [51] Int. Cl B01k 5/00 [58] Field. of Search 204/180 G, 180 S, 299
[56] References Cited UNITED STATES PATENTS 3,317,418 5/1967 Zec 204/299 3,428,547 2/1969 Zec 204/299 3,616,387 10/1971 Siebert et al 204/180 G OTHER PUBLICATIONS Houtsmuller, A Simple Device for Applying Serum to Viscous Media, Clin. Chim. Acta., 10, (1964), pp. 94 & 95. l-Ioutsmuller, "Agarose-Gel-EIectrophoresis of. Lipoproteins, Charles C. Thomas publisher, 1969, pp. 13-15.
Primary Examiner.l0hn H. Mack Assistant ExaminerA. C. Prescott Attorney, Agent, or FirmR. .I. Steinmeyer; J. G. Mesaros 5 7] ABSTRACT This invention provides an apparatus and applicator for applying liquid sample to an electrophoretic membrane. The applicator comprises two parallel strips of flexible plastic film separated by a spacer along one edge. At the other edge the strips form fingers between which are suspended liquid samples. The appli cator is gripped at the laminated edge by the apparatus at the end of an arm pivotally mounted on a base plate. The arm is connected by mechanical linkage to a handle which controls movement of the arm such that the applicator can be lowered to contact a membrane supported by the base plate with the correct amount of force to deposit the liquid samples cleanly and precisely on the membrane. The gripping element of the apparatus can be adjusted to control the spacing between the fingers and thereby control the amount of sample deposited.
14 Claims, 4 Drawing Figures PAIENIED sumaer 4 FIG. 2
1 ELECTROPHORESIS SAMPLE APPLICATOR BACKGROUND OF THE INVENTION Certain analytical procedures, such as electrophoresis, require the depositing of precise amounts of liquid sample, such as serum, at precise places on a gel or membrane resting on a carrier plate or slide. In the case of a gel, the sample is usually deposited manually, such as by pipetting the sample into preformed cavities or by applying the sample with a spatula (as described in US. Pat. No. 3,622,484) or other hand-held device. In either event, the operation is tedious, time consuming, requires manual skill, and is subject to operator error, with the result that frequently either too much or too little sample is deposited, or the sample is smeared or splattered on the gel or membrane. It is an object of this invention to provide an improved method, apparatus and applicator for applying liquid sample to a gel or membrane.
SUMMARY OF THE INVENTION This invention provides an apparatus and applicator for applying liquid sample, such as blood serum, to a membrane, such as an agarose gel electrophoretic membrane. The applicator is gripped by the apparatus at the end of an arm pivotally mounted on a base plate. The arm is mechanically connected to a handle such that rotation of the handle results in rotation of the arm, whereby, when a membrane is placed on the base plate, rotation of the handle causes the applicator to contact the membrane and release sample contained by the applicator. In a preferred embodiment, the means for gripping the applicator includes means for adjusting the horizontal alignment of the applicator and means for adjusting the sample holding capacity of the applicator. The applicator comprises two strips of flexible film separated at one edge by a spacer laminated between the strips, with each strip having a plurality of fingers at the opposite edge which define capillary spaces between the strips for containing liquid sample. The apparatus and applicator of the invention overcome the problems inherent in the prior art methods of applying sample manually.
BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a side view of the apparatus of this invention showing the arm in a slightly raised position with the applicator gripped at the end of the arm.
FIG. 2 is an exploded view of the apparatus of this invention.
FIG. 3 is a front view of an applicator used with the apparatus.
FIG. 4 is a side view of the applicator.
DETAILED DESCRIPTION The apparatus comprises an arm pivotally mounted between two support blocks 11 secured to a base plate 12 by screws 13. The arm 10 pivots on bushings 14 about pins 15. Also pivotally mounted between the support blocks 11 is a handle 16, which pivots on bushings 17 about pins 18. The handle 16 is connected to the arm 10 through a link 19. The link 19 is secured to the handle 16 by pin 20 and to the arm 10 by pin 21 at places offset from the axes of rotation such that as the arm 10 approaches a horizontal position, a large angular movement of the handle 16 results in a cone spondingly smaller angular movement of the arm 10.
This ensures that the applicator enters and leaves the gel in aslow, deliberate motion so that even an untrained operator can make a precise application of sample each time.
One end of the arm 10 terminates in a face 22. A jaw 23 contacts the face 22 and is held in place by an adjusting knob 24 which threadedly engages a screw 25 passing through the face 22. The knob 24 has a spherical base which engages a spherical depression in the jaw 23. The lower edge of the jaw 23 forms a lip 26. A spring 27 biases the upper portion of the jaw 23 away from the face 22. A fin extending from the jaw 23 engages a slot 28 in the face 22 and thereby limits the rotational freedom of the jaw 23. However, the jaw 23 is free to rotate in a plane perpendicular to the surface of the face 22 about an axis passing through the knob 24.
The applicator comprises two strips 40 of flexible film, such as plastic (e.g., polyester) film, separated at the top by a spacer 41 laminated between the strips. The inner surfaces of the strips are preferably coated with a hydrophilic material, such as gelatin. The outer surfaces are preferably hydrophobic. Each strip 40 has a plurality of fingers 42 which define capillary spaces between the strips for containing the liquid samples, which are released when the fingers contact the gel. The fingers of one strip are preferably slightly longer than the fingers of the other strip so that the fingers cut and enter the gel cleanly. If the fingers were the same length, they would tend to crush the gel rather than pierce it. The shorter fingers preferably have holes 43 in them to facilitate loading of sample.
The applicator is positioned between the jaw 23 and the face 22 by pushing the upper portion ofjaw 23 toward the face 22 to move the lip 26 away from the face, and then inserting the applicator such that a notch 44 in the top of the applicator engages a protrusion 29 on the face 22, followed by releasing the upper portion of jaw 23 to secure the applicator between the lip 26 and the face 22. The protrusion 29 fixes the lateral position of the applicator while permitting it to rotate so that it can be aligned horizontally.
The applicator is aligned horizontally by lowering the arm 10 until the tips of the fingers 42 touch a slide (not shown) resting on the base plate 12. As the fingers 42 touch the slide, the grip on the applicator is relaxed briefly by squeezing the upper portion of the jaw 23 toward the face 22, which permits the tips of the fingers to align horizontally on the surface of the slide.
After the fingers 42 have been aligned, the spacing between the strips may be adjusted to control the amount of sample contained between the fingers. The spacing is adjusted by rotating the adjusting knob 24. When the knob 24 is rotated completely in one direction, the surface of the jaw 23 is parallel to the surface of the face 22. The strips 40 of the applicator are then parallel and the space between them is equal to the thickness of the spacer 41. When the knob 24 is rotated in the other direction, the jaw 23 becomes angular with respect to the face 22. Since the front fingers rest against the lip 26 of the jaw 23, while the back fingers rest against the surface of the face 22, as the jaw becomes angular the front fingers become bent toward the back fingers. Thus, the lower edges of the strips 40 may be made closer or farther apart depending on the setting of the knob 24, with the result that a repeatable amount of sample can be deposited in the gel with a minimum of operator skill.
After the fingers 42 have been aligned and the space between them adjusted, liquid sample is introduced into the space between the fingers of the adjacent strips. A particularly convenient method of introducing sample is to dip the fingers into a trough divided into compartments corresponding to the fingers. Each compartment contains a separate sample for each pair of fingers. The trough is placed on the base plate 12 and the arm is lowered so that each finger enters its respective compartment of the trough. The samples flow into the spaces between the fingers by capillary action. Alternatively, sample may be introduced into the spaces by using a pipette.
The samples are deposited into a gel by placing the membrane on a slide resting on the base plate 12, and lowering the arm 10 by rotating the handle 16 until the fingers 42 contact the gel. As the fingers contact the gel, the sample is released.
The base plate 12 has two keypins for accurately positioning the slide by engaging holes in the bottom of the slide. As a result, the samples are automatically deposited precisely where desired in the gel. Slides with different keyhole patterns can be used for various application requirements. The keypins can also be used to position the sample trough.
The applicator illustrated herein is especially suitable for zone electrophoresis. However, other applicators,
- such as for immunoelectrophoresis. can also be employed in accordance with this invention. The jaw and face of the apparatus can, of course, be modified as necessary to accommodate other applicators. Similarly, the apparatus of this invention can be used to apply samples in applications other than electrophoresis. such as paper chromatography.
What is claimed is:
1. Apparatus for applying liquid sample to a membrane. comprising a base plate,
an arm pivotally mounted on one end and supported by the base plate.
means at the other end of the arm for gripping a sample applicator.
a handle connected by a mechanical linkage to the arm such that rotation of the handle results in rotation of the arm. whereby. when a membrane is placed on the base plate. rotation of the handle causes the applicator to contact the membrane and release sample contained by the applicator.
2. The apparatus of claim 1 wherein the gripping means includes means for adjusting the horizontal alignment of the applicator.
3. The apparatus of claim 1 wherein the gripping means includes means for adjusting the sample holding capacity of the applicator.
4. The apparatus of claim 1 wherein the mechanical connection between the handle and the arm is such that a large rotation of the handle results in a correspondingly smaller rotation of the arm as the arm approaches a horizontal position.
I 5. The apparatus of claim 1 wherein the base plate includes keypins for accurately positioning the membrane.
6. The apparatus of claim 1 wherein the gripping means comprises a face at the end of the arm,
an adjusting knob threadedly engaged to the face,
a jaw between the face and the knob, the jaw being free to rotate in a plane perpendicular to the surface of the face about an axis passing through the adjusting knob.
means biasing the upper portion of the jaw away from the face.
7. The apparatus of claim 6 wherein the face includes a protrusion for fixing the lateral position of the applicator.
8. The apparatus of claim 1 in combination with a sample applicator comprising two substantially parallel strips of flexible film separated at one edge by a spacer laminated between the strips, each strip having a plurality of fingers at the opposite edge which define capillary spaces between the strips for containing liquid sample.
9. The combination of claim 8 wherein the. applicator has a notch at the laminated edge for engaging a protrusion in the gripping means for fixing lateral motion of the applicator.
10. A sample applicator comprising two substantially parallel strips of a flexible film separated at one edge by a spacer laminated between the strips, each strip having a plurality of fingers at the opposite edge which define capillary spaces between the strips for containing liquid sample.
11. The applicator of claim 10 wherein the fingers of one strip are slightly longer than the fingers of the other strip.
12. The applicator of claim 11 wherein each shorter finger has a hole in it to facilitate loading of sample.
13. The applicator of claim 12 wherein the inner surfaces of the strips are hydrophilic.
14. The applicator of claim 13 having a notch at the laminated edge adapted to engage a protrusion for fixing lateral motion of the applicator.
l l l

Claims (14)

1. Apparatus for applying liquid sample to a membrane, comprising a base plate, an arm pivotally mounted on one end and supported by the base plate, means at the other end of the arm for gripping a sample applicator, a handle connected by a mechanical linkage to the arm such that rotation of the handle results in rotation of the arm, whereby, when a membrane is placed on the base plate, rotation of the handle causes the applicator to contact the membrane and release sample contained by the applicator.
2. The apparatus of claim 1 wherein the gripping means includes means for adjusting the horizontal alignment of the applicator.
3. The apparatus of claim 1 wherein the gripping means includes means for adjusting the sample holding capacity of the applicator.
4. The apparatus of claim 1 wherein the mechanical connection between the handle and the arm is such that a large rotation of the handle results in a correspondingly smaller rotation of the arm as the arm approaches a horizontal position.
5. The apparatus of claim 1 wherein the base plate includes keypins for accurately positioning the membrane.
6. The apparatus of claim 1 wherein the gripping means comprises a face at the end of the arm, an adjusting knob threadedly engaged to the face, a jaw between the face and the knob, the jaw being free to rotate in a plane perpendicular to the surface of the face about an axis passing through the adjusting knob, means biasing the upper portion of the jaw away from the face.
7. The apparatus of claim 6 wherein the face includes a protrusion for fixing the lateral position of the applicator.
8. The apparatus of claim 1 in combination with a sample applicator comprising two substantially parallel strips of flexible film separated at one edge by a spacer laminated between the strips, each strIp having a plurality of fingers at the opposite edge which define capillary spaces between the strips for containing liquid sample.
9. The combination of claim 8 wherein the applicator has a notch at the laminated edge for engaging a protrusion in the gripping means for fixing lateral motion of the applicator.
10. A sample applicator comprising two substantially parallel strips of a flexible film separated at one edge by a spacer laminated between the strips, each strip having a plurality of fingers at the opposite edge which define capillary spaces between the strips for containing liquid sample.
11. The applicator of claim 10 wherein the fingers of one strip are slightly longer than the fingers of the other strip.
12. The applicator of claim 11 wherein each shorter finger has a hole in it to facilitate loading of sample.
13. The applicator of claim 12 wherein the inner surfaces of the strips are hydrophilic.
14. The applicator of claim 13 having a notch at the laminated edge adapted to engage a protrusion for fixing lateral motion of the applicator.
US00370348A 1973-06-15 1973-06-15 Electrophoresis sample applicator Expired - Lifetime US3839183A (en)

Priority Applications (10)

Application Number Priority Date Filing Date Title
US00370348A US3839183A (en) 1973-06-15 1973-06-15 Electrophoresis sample applicator
CA199,521A CA1005256A (en) 1973-06-15 1974-05-10 Pivoted arm with flexible strip electrophoresis sample applicator
GB2237474A GB1446125A (en) 1973-06-15 1974-05-20 Electrophoresis sample applicator
IT23155/74A IT1012799B (en) 1973-06-15 1974-05-24 LIQUID SAMPLE APPLICATOR ON ELECTROPHORETIC MEMBRANE
DK313674A DK313674A (en) 1973-06-15 1974-06-12
JP1974068010U JPS5434635Y2 (en) 1973-06-15 1974-06-13
SE7407911A SE7407911L (en) 1973-06-15 1974-06-14
DE2428829A DE2428829A1 (en) 1973-06-15 1974-06-14 DEVICE FOR APPLYING A LIQUID SAMPLE AND APPLICATOR FOR USE IN THIS DEVICE
CH817974A CH580275A5 (en) 1973-06-15 1974-06-14
SE7810453A SE7810453L (en) 1973-06-15 1978-10-05 APPLIANCE FOR APPLICATION OF LIQUID SAMPLES

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US00370348A US3839183A (en) 1973-06-15 1973-06-15 Electrophoresis sample applicator

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US3839183A true US3839183A (en) 1974-10-01

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JP (1) JPS5434635Y2 (en)
CA (1) CA1005256A (en)
CH (1) CH580275A5 (en)
DE (1) DE2428829A1 (en)
DK (1) DK313674A (en)
GB (1) GB1446125A (en)
IT (1) IT1012799B (en)
SE (2) SE7407911L (en)

Cited By (20)

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US4199428A (en) * 1978-04-27 1980-04-22 Olympus Optical Co., Ltd. Sample feeder for use in automatic electrophoresis system
US4214973A (en) * 1977-12-15 1980-07-29 Olympus Optical Co., Ltd. Blood serum applicator for use in cataphoretic apparatus
US4257868A (en) * 1979-03-30 1981-03-24 Olympus Optical Co., Ltd. Automatic serum applicator with serum drying-preventive mechanism
US4297199A (en) * 1979-11-14 1981-10-27 Olympus Optical Company Limited Specimen applicator
US5275710A (en) * 1990-05-14 1994-01-04 Labintelligence, Inc. Gel electrophoresis system including optical stage, sample applicator and sample retriever
US5405516A (en) * 1991-01-04 1995-04-11 Sebia Apparatus for the application of biological samples to an electrophoretic slab support
US5464515A (en) * 1991-01-04 1995-11-07 Sebia, A Corp. Of France Apparatus for the application of biological samples to an electrophoretic slab support
US5681437A (en) * 1995-10-31 1997-10-28 Isolab, Inc. Sample deposition device
US5683915A (en) * 1995-10-31 1997-11-04 Isolab, Inc. Sample deposition device and method
US5968331A (en) * 1994-11-11 1999-10-19 Hitachi, Ltd. Sample holding device for electrophoresis apparatus and sample injection method
US5993627A (en) * 1997-06-24 1999-11-30 Large Scale Biology Corporation Automated system for two-dimensional electrophoresis
US6027625A (en) * 1996-05-17 2000-02-22 Purdue Research Foundation Miniaturized disposable gels for DNA analysis
EP1007955A1 (en) 1996-05-06 2000-06-14 Helena Laboratories Corporation Apparatus for the application of samples on a substrate
EP1068526A1 (en) * 1997-12-09 2001-01-17 Amersham Pharmacia Biotech Inc Article for transporting biological samples during analysis
US6554991B1 (en) 1997-06-24 2003-04-29 Large Scale Proteomics Corporation Automated system for two-dimensional electrophoresis
WO2005068992A1 (en) * 2004-01-14 2005-07-28 Interlab S.R.L. Applicator of fluid samples on a substrate for chemical analysis, in particular for electrophoresis analyses
US20060099114A1 (en) * 2004-06-21 2006-05-11 Jeremy Caldwell Gel extraction device
WO2009091320A1 (en) * 2008-01-17 2009-07-23 Ge Healthcare Bio-Sciences Ab Method for sample application
US8163153B2 (en) 2005-12-20 2012-04-24 Caldwell Jeremy S Tool for extracting electrophoretic sample
US20210025848A1 (en) * 2016-07-29 2021-01-28 Vital Biosciences, Inc. Gel electrophoresis diagnostic kit and methods of using the same

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CN105510103A (en) * 2016-02-01 2016-04-20 安徽理工大学 Coal briquette sample preparation mold

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US3428547A (en) * 1965-07-02 1969-02-18 Beckman Instruments Inc Antiserum applicator used in immunoelectrophoresis
US3616387A (en) * 1969-11-13 1971-10-26 Bio Rad Laboratories Method and apparatus for the transfer of fluid samples

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Cited By (39)

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US4214973A (en) * 1977-12-15 1980-07-29 Olympus Optical Co., Ltd. Blood serum applicator for use in cataphoretic apparatus
US4199428A (en) * 1978-04-27 1980-04-22 Olympus Optical Co., Ltd. Sample feeder for use in automatic electrophoresis system
US4257868A (en) * 1979-03-30 1981-03-24 Olympus Optical Co., Ltd. Automatic serum applicator with serum drying-preventive mechanism
US4297199A (en) * 1979-11-14 1981-10-27 Olympus Optical Company Limited Specimen applicator
US5275710A (en) * 1990-05-14 1994-01-04 Labintelligence, Inc. Gel electrophoresis system including optical stage, sample applicator and sample retriever
US5405516A (en) * 1991-01-04 1995-04-11 Sebia Apparatus for the application of biological samples to an electrophoretic slab support
US5464515A (en) * 1991-01-04 1995-11-07 Sebia, A Corp. Of France Apparatus for the application of biological samples to an electrophoretic slab support
US5968331A (en) * 1994-11-11 1999-10-19 Hitachi, Ltd. Sample holding device for electrophoresis apparatus and sample injection method
US5681437A (en) * 1995-10-31 1997-10-28 Isolab, Inc. Sample deposition device
US5683915A (en) * 1995-10-31 1997-11-04 Isolab, Inc. Sample deposition device and method
EP1007955A1 (en) 1996-05-06 2000-06-14 Helena Laboratories Corporation Apparatus for the application of samples on a substrate
US6027625A (en) * 1996-05-17 2000-02-22 Purdue Research Foundation Miniaturized disposable gels for DNA analysis
US6554991B1 (en) 1997-06-24 2003-04-29 Large Scale Proteomics Corporation Automated system for two-dimensional electrophoresis
US6482303B2 (en) * 1997-06-24 2002-11-19 Large Scale Proteomics Corp. Automated system for two-dimensional electrophoresis
US6136173A (en) * 1997-06-24 2000-10-24 Large Scale Biology Corporation Automated system for two-dimensional electrophoresis
US20050126911A1 (en) * 1997-06-24 2005-06-16 Large Scale Biology Corporation Automated system for two-dimensional electrophoresis
US6245206B1 (en) 1997-06-24 2001-06-12 Large Scale Biology Corp. Automated system for two-dimensional electrophoresis
US20010023826A1 (en) * 1997-06-24 2001-09-27 Large Scale Biology Corporation Automated system for two dimensional electrophoresis
US6643391B2 (en) 1997-06-24 2003-11-04 Large Scale Proteomics Corporation Apparatus for computer-assisted isolation and characterization of proteins
US6391650B1 (en) 1997-06-24 2002-05-21 Large Scale Biology Corporation Protein sample preparation for electrophoresis
US6398932B1 (en) 1997-06-24 2002-06-04 Large Scale Proteomics Corp. Automated system for two-dimensional electrophoresis
US6416644B1 (en) 1997-06-24 2002-07-09 Large Scale Proteomics Corp. Automated system for two-dimensional electrophoresis
US6438259B1 (en) 1997-06-24 2002-08-20 Large Scale Biology Corporation Computer-assisted methods and apparatus for identification and characterization of biomolecules
US6451189B2 (en) 1997-06-24 2002-09-17 Large Scale Proteomics Corp. Automated system for two dimensional electrophoresis
US20020157954A1 (en) * 1997-06-24 2002-10-31 Anderson N. Leigh Automated system for two-dimensional electrophoresis
US6123821A (en) * 1997-06-24 2000-09-26 Large Scale Biology Corporation Automated system for two-dimensional electrophoresis
US6507664B1 (en) 1997-06-24 2003-01-14 Large Scale Biology Corporation Two-dimensional gels for separation, identification and characterization of biomolecules
US5993627A (en) * 1997-06-24 1999-11-30 Large Scale Biology Corporation Automated system for two-dimensional electrophoresis
EP1068526A4 (en) * 1997-12-09 2002-05-15 Amersham Pharm Biotech Inc Article for transporting biological samples during analysis
EP1068526A1 (en) * 1997-12-09 2001-01-17 Amersham Pharmacia Biotech Inc Article for transporting biological samples during analysis
WO2005068992A1 (en) * 2004-01-14 2005-07-28 Interlab S.R.L. Applicator of fluid samples on a substrate for chemical analysis, in particular for electrophoresis analyses
US20060099114A1 (en) * 2004-06-21 2006-05-11 Jeremy Caldwell Gel extraction device
US7413908B2 (en) * 2004-06-21 2008-08-19 Jeremy Caldwell Gel extraction device
US8163153B2 (en) 2005-12-20 2012-04-24 Caldwell Jeremy S Tool for extracting electrophoretic sample
WO2009091320A1 (en) * 2008-01-17 2009-07-23 Ge Healthcare Bio-Sciences Ab Method for sample application
US20100282610A1 (en) * 2008-01-17 2010-11-11 Ge Healthcare Bio-Sciences Ab Method for sample application
US8470148B2 (en) 2008-01-17 2013-06-25 Ge Healthcare Bio-Sciences Ab Method for sample application
US20210025848A1 (en) * 2016-07-29 2021-01-28 Vital Biosciences, Inc. Gel electrophoresis diagnostic kit and methods of using the same
US11692967B2 (en) * 2016-07-29 2023-07-04 Vital Biosciences Inc. Gel electrophoresis diagnostic kit and methods of using the same

Also Published As

Publication number Publication date
DE2428829A1 (en) 1975-01-09
GB1446125A (en) 1976-08-18
JPS5058790U (en) 1975-05-31
SE7810453L (en) 1978-10-05
IT1012799B (en) 1977-03-10
SE7407911L (en) 1974-12-16
JPS5434635Y2 (en) 1979-10-23
CA1005256A (en) 1977-02-15
CH580275A5 (en) 1976-09-30
DK313674A (en) 1975-03-17

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