US3766016A - Apparatus for distributing a liquid volume into a number of receptacles - Google Patents

Apparatus for distributing a liquid volume into a number of receptacles Download PDF

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Publication number
US3766016A
US3766016A US00087681A US3766016DA US3766016A US 3766016 A US3766016 A US 3766016A US 00087681 A US00087681 A US 00087681A US 3766016D A US3766016D A US 3766016DA US 3766016 A US3766016 A US 3766016A
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United States
Prior art keywords
tubes
liquid
channel
vessels
portions
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Expired - Lifetime
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US00087681A
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English (en)
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J Guigan
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G01N1/18—Devices for withdrawing samples in the liquid or fluent state with provision for splitting samples into portions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0439—Rotary sample carriers, i.e. carousels
    • G01N2035/0446—Combinations of the above
    • G01N2035/0449—Combinations of the above using centrifugal transport of liquid

Definitions

  • ABSTRACT An analytical device for distributing equal portions of a certain liquid volume into a plurality of vessels, the device having a groove-shaped circular channel with the partially communicating vessels attached to its outer rim. Sufficient liquid is fed to the channel and allowed to overflow into the communicating parts of the vessels from where the individual portions of the liquid are forced into the non-communicating parts of the vessels by rotation of the device.
  • This invention is concerned with an apparatus for the simultaneous distribution of a given volume of a liquid between a plurality of vessels so that each of said vessels receives a substantially equal volume of liquid.
  • the identification of a bacteria is conventionally undertaken by distributing a culture medium, which has undergone a suitable incubation, between a plurality of vessels, each of which contains a different reagent.
  • the vessels used are test-tubes, capillary tubes or hollows formed in a sheet of plastic.
  • An alternative method is to establish the antibiogram of the bacteria by distributing, in the same manner, a solution of the bacteria between vessels containing different antibiotics in a variety of doses.
  • the media have in the past conventionally been seeded one by one but since there are a large number of different media this operation was a long and delicate one.
  • an apparatus for dividing a given volume of liquid into a plurality of substantially equal portions which comprises a plurality of tubes closed at one end and having an opening at the other end, the open ends of the tubes communicating with a common circular channel, the said circular channel being capable of communicating by means of radially directed conduits with a receptacle containing the liquid to be divided, the tubes being disposed substantially radially with respect to the said channel and the said tubes having two portions which form an obtuse angle with respect to each other.
  • FIG. 1 is a cross-sectional elevation of an apparatus according to the present invention
  • FIG. 2 is a plan of the apparatus of FIG. 1 and FIG. 3 is a plan ofa variation of the apparatus shown in FIG. 1.
  • the apparatus is in the general form of a disc and, in use, must be in the horizontal position.
  • the apparatus consists essentially of a circular channel 10, which is interconnected to a plurality of tubes 11, the said tubes being closed at their ends away from the channel 10.
  • the tubes 11 are disposed radially in relation to the channel 10.
  • the tubes have a portion thereof 110 which is inclined upwardly from the horizontal and which leads to a portion thereof 111 which is inclined downwardly.
  • the obtuse angle between the two portions of the tubes may conveniently be from 150 to nearly 180.
  • the tubes are so disposed that the uppermost level of the channel is situated higher than the lowest point of the tube portion 110.
  • the circular channel 10 is connected by a series of radially disposed conduits (e.g., 20) with a central container 21 through which the liquid to be distributed can be introduced.
  • the conduits 20, the channel 10 and the tubes 11 are preferably closed by a plastic covering which renders the assembly hermetically sealed.
  • the central container 21 has, in its lower part, an opening 22 to which may be attached a small bulb 23 which is made of a flexible material such as rubber or a flexible plastic.
  • the entire disc assembly comprising the channel 10, the conduits 20, the central container 21 and the tubes 11 may conveniently be made out of plastic and be formed as a single piece obtained by moulding or casting.
  • the assembly is sterilised and into each of the downwardly inclined portions 111 of the tubes 11 there is introduced a culture medium 112 containing a reagent.
  • the bulb 23 is detached from its place. A suitable quantity of the solution is then sucked into the bulb which is then replaced on the apparatus. The bulb is then squeezed upwardly so that the liquid is forced into the receptacle 21 and passes, via the conduits 20, into the channel 10.
  • the liquid After having filled the channel 10 the liquid then overflows into the upwardly inclined portions of the radially directed tubes 11.
  • the entire assembly consisting of the disc, tubes and bulb is then rotated about the planar axis of the disc. This movement may be limited to a fraction of a turn or may be more than several turns.
  • the rotation may be conveniently effected by placing the assembly of the disc and bulb on a support which rotates in the horizontal plane. Under the effect of the resulting centrifugal force the liquid present in the upwardly inclined portions 110 of the tubes 11 passes over into the downwardly inclined portions 111 which contain the culture media 112 and the reagents associated therewith.
  • the degree of growth of the bacteria is measured by observing the colouration of the reagents contained in each of the tubes 11.
  • a further possibility is to employ a certain proportion of the tubes for the identification of the bacteria and to employ the rest for the determination of the antiboigram.
  • FIG. 3 of the accompanying drawings there is shown a plan view of a portion of a disc assembly which is somehwat modified as compared with that shown in FlGS. 1 and 2, in that the tubes are not disposed perpendicularly with respect to the channel 110 but at an angle other than at a right angle.
  • the tubes may thus conveniently be disposed at an angle of from 90 to 120 with respect to the tangent of the circular channel.
  • This modified configuration facilitates the passage of the liquid from the zone 110 into the zone Ill during the rotational movement, on account of the inertia of the drops of liquid.
  • the tubes 11 are not rectilinear but curved.
  • the apparatus also has the advantage that the identification of a sample is only made once with respect to any part of the disc, as compared with the methods previously used in which it was necessary to identify individually each one of the tubes.
  • An apparatus for dividing a given volume of liquid into a plurality of substantially equal portions which comprises; a common horizontal circular channel, a plurality of tubes closed at one end and having an opening at the other end, the open ends of the tubes communicating with said common horizontal circular channel, radially directed conduits fluid connecting said channel with a receptacle containing the liquid to be divided, said tubes being disposed substantially radially with respct to said channel at circumferentially spaced positions and having a first inner portion extending radially outwards from the outer edge of said circular channel with its inboard end lying below the level of said edge, said first tube portions being inclined upwardly and terminating in second tube portions inclined downwardly and forming with said first portions an obtuse angle whereby a given volume of liquid is captured at the inboard end of each first tube section by liquid overflow of the outer lip of said circular channel permitting the captured given volume of liquid to

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  • Life Sciences & Earth Sciences (AREA)
  • Hydrology & Water Resources (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Sampling And Sample Adjustment (AREA)
US00087681A 1969-11-12 1970-11-09 Apparatus for distributing a liquid volume into a number of receptacles Expired - Lifetime US3766016A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR6938776A FR2067639A5 (enrdf_load_stackoverflow) 1969-11-12 1969-11-12

Publications (1)

Publication Number Publication Date
US3766016A true US3766016A (en) 1973-10-16

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Family Applications (1)

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US00087681A Expired - Lifetime US3766016A (en) 1969-11-12 1970-11-09 Apparatus for distributing a liquid volume into a number of receptacles

Country Status (8)

Country Link
US (1) US3766016A (enrdf_load_stackoverflow)
JP (1) JPS5018798B1 (enrdf_load_stackoverflow)
CA (1) CA919630A (enrdf_load_stackoverflow)
CH (1) CH515500A (enrdf_load_stackoverflow)
FR (1) FR2067639A5 (enrdf_load_stackoverflow)
GB (1) GB1305215A (enrdf_load_stackoverflow)
NL (1) NL149288B (enrdf_load_stackoverflow)
SE (1) SE367486B (enrdf_load_stackoverflow)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3986534A (en) * 1974-07-30 1976-10-19 Intertechnique S.A. Device for measuring and dispensing fractionary volumes of liquid samples
US4743558A (en) * 1984-10-26 1988-05-10 Jean Guigan Method of performing medical analysis on a sample of liquid by means of at least one liquid reagent, and apparatus for performing the method
US20130174672A1 (en) * 2011-11-20 2013-07-11 PIETER VAN WEENEN & CO. GmbH, The House of Innovation Distributor Device for a Rehearse Exposure Arrangement

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH685312A5 (de) * 1993-05-03 1995-05-31 Diag Human Ag Vorrichtung zur Blutuntersuchung.

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3175732A (en) * 1960-07-28 1965-03-30 Unger Hans Peter Olof Device for measuring and dividing of liquids
US3575220A (en) * 1968-08-12 1971-04-20 Scientific Industries Apparatus for dispensing liquid sample

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3175732A (en) * 1960-07-28 1965-03-30 Unger Hans Peter Olof Device for measuring and dividing of liquids
US3575220A (en) * 1968-08-12 1971-04-20 Scientific Industries Apparatus for dispensing liquid sample

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3986534A (en) * 1974-07-30 1976-10-19 Intertechnique S.A. Device for measuring and dispensing fractionary volumes of liquid samples
US4743558A (en) * 1984-10-26 1988-05-10 Jean Guigan Method of performing medical analysis on a sample of liquid by means of at least one liquid reagent, and apparatus for performing the method
US20130174672A1 (en) * 2011-11-20 2013-07-11 PIETER VAN WEENEN & CO. GmbH, The House of Innovation Distributor Device for a Rehearse Exposure Arrangement
US9329110B2 (en) * 2011-11-20 2016-05-03 PIETER VAN WEENEN & CO. GmbH, The House of Innovation Apparatus for distributing fluid to a plurality of sample containers

Also Published As

Publication number Publication date
NL149288B (nl) 1976-04-15
SE367486B (enrdf_load_stackoverflow) 1974-05-27
GB1305215A (enrdf_load_stackoverflow) 1973-01-31
FR2067639A5 (enrdf_load_stackoverflow) 1971-08-20
DE2055304B2 (de) 1975-10-23
DE2055304A1 (de) 1971-05-19
CH515500A (de) 1971-11-15
NL7016295A (enrdf_load_stackoverflow) 1971-05-14
CA919630A (en) 1973-01-23
JPS5018798B1 (enrdf_load_stackoverflow) 1975-07-02

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