US3752146A - Centrifuge tube for ultra-violet analysis - Google Patents
Centrifuge tube for ultra-violet analysis Download PDFInfo
- Publication number
- US3752146A US3752146A US00207192A US20719271A US3752146A US 3752146 A US3752146 A US 3752146A US 00207192 A US00207192 A US 00207192A US 20719271 A US20719271 A US 20719271A US 3752146 A US3752146 A US 3752146A
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- United States
- Prior art keywords
- centrifuge tube
- optical fiber
- fiber element
- tube
- ultra
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- Expired - Lifetime
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- 239000013307 optical fiber Substances 0.000 claims abstract description 19
- 239000012528 membrane Substances 0.000 claims abstract description 11
- 229920003023 plastic Polymers 0.000 claims abstract description 9
- 239000000463 material Substances 0.000 claims abstract description 8
- 239000010453 quartz Substances 0.000 claims abstract description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 5
- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000007787 solid Substances 0.000 claims description 14
- 239000004033 plastic Substances 0.000 claims description 7
- -1 polyethylene Polymers 0.000 claims description 6
- 239000004698 Polyethylene Substances 0.000 claims description 3
- 239000004743 Polypropylene Substances 0.000 claims description 3
- 238000004891 communication Methods 0.000 claims description 3
- 238000000465 moulding Methods 0.000 claims description 3
- 229920000573 polyethylene Polymers 0.000 claims description 3
- 229920001155 polypropylene Polymers 0.000 claims description 3
- 229920002635 polyurethane Polymers 0.000 claims description 3
- 239000004814 polyurethane Substances 0.000 claims description 3
- 230000005540 biological transmission Effects 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 4
- 230000000007 visual effect Effects 0.000 abstract description 2
- 239000000975 dye Substances 0.000 description 5
- 230000005856 abnormality Effects 0.000 description 2
- 230000002380 cytological effect Effects 0.000 description 2
- 238000002405 diagnostic procedure Methods 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 230000001744 histochemical effect Effects 0.000 description 2
- 238000009595 pap smear Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 208000006105 Uterine Cervical Neoplasms Diseases 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 229920002457 flexible plastic Polymers 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 239000002991 molded plastic Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000009827 uniform distribution Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
- A61B10/02—Instruments for taking cell samples or for biopsy
- A61B10/0291—Instruments for taking cell samples or for biopsy for uterus
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5021—Test tubes specially adapted for centrifugation purposes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/07—Centrifugal type cuvettes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S385/00—Optical waveguides
- Y10S385/901—Illuminating or display apparatus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/2575—Volumetric liquid transfer
Definitions
- the optical fiber element may be formed from UNITED STATES PATENTS quartz or from such a material as a methyl methacry- 3,712,295 1/1973 Kline 128/2 F late capable of transmitting ultrawiolet light 3,480,398 11/1969 Hamilton 23/259 X 3,327,!
- This invention relates to new and useful improvements in laboratory equipment and particularly seeks to provide a novel molded centrifuge tube formed from that type of plastic that is transparent upon completion of molding so that the contents of the tube, during use thereof, may be subjected to ultra-violet light analysis without having to be transferred to a separate container or receiving slide.
- the area to be tested generally is irrigated with a pre-or post-dyed saline or other solution which is then withdrawn and centrifuged to separate the solids for subsequent reagent or other analysis.
- a pre-or post-dyed saline or other solution which is then withdrawn and centrifuged to separate the solids for subsequent reagent or other analysis.
- Such an analysis may include subjecting the dyed specimen solids to exposure to ultra-violet light to visually indicate the qualitative presence or absence of cytological and histo-chemical abnormalities in the specimen by color changes as compared to a control.
- the dyes are selected to be effective for the precise type of ultra-violet indication required. That is to say, one dye or a class of dyes may give an ultraviolet response to cancerous matter, for example, while another dye or class of dyes may give an ultra-violet response to another type of detectable matter in the specimen.
- a centrifuge tube constructed in accordance with this invention enables such procedures to be greatly simplified in that the same tube may be used to collect the specimen, centrifuge the specimen and subject the specimen to ultra-violet light analysis without having to transfer any portion of the centrifuged specimen to a slide or other external receiver.
- the effectiveness of the centrifuge tube of this invention stems from the fact that it is transparent and is pro vided at its bottom with a membrane closure that can be penetrated by a sharp ended optical fiber element that is capable of transmitting ultra-violet light from an external source into the bottom portion of the tube upon completion of centrifuging.
- Such an optical fiber element may be formed from quartz or from any other material capable of transmitting ultra-violet light, such as a methyl methacrylate of the type commercially available from Imperial Chemical Industries under the Trademark DlAKON, or its equivalent.
- an object of this invention is to provide a transparent centrifuge tube formed from asmooth, inert, flexible plastic having a memory, and having a syringe tip at its top and a sealed bottom that is penetrable by a sharp ended optical fiber element capable of transmitting ultra-violet light to the interior of said tube.
- Another object of this invention is to provide a centrifuge tube of the character stated in which the body is formed as a bellows-folded helix to direct the flow of solid particles to the bottom when centrifuged.
- Another object of this invention is to provide a centrifuge tube of the character stated in which said optical fiber element is in the form of a double tipped hollow needle to enable said tube to be used as a positive acting syringe to discharge the concentrated solids for subsequent examination following completion of centrifuging and ultra-violet light analysis of the contents.
- a further object of this invention is to provide a centrifuge tube of the character stated in which the sealed bottom is tapered by two diametrically opposed concave hollows that may serve as abutments for the positioning of the tube in a centrifuge and/or as abutments for the mechanical axial partial collapsing of the tube in subsequently used processing apparatus.
- a further object of this invention is to provide a centrifuge tube of the character stated that is simple in design, rugged in construction and economical to manufacture.
- FIG. 1 is a side elevation of a centrifuge tube constructed in accordance with this invention
- FIG. 2 is a fragmentary section of the bottom thereof showing the insertion of a sharp ended optical fiber element through the bottom for ultra-violet light analysis of the centrifuged contents;
- FIG. 3 is a detail elevation, partly in section, showing a modified form of optical fiber element to permit the tube to be used as a syringe for discharge of at least a portion of the contents.
- a transparent centrifuge tube generally indicated 5 formed from an inert molded plastic such as a polyethylene, polypropylene or polyurethane having a memory and having the characteristic ,of transparency when molded into a finished object and includes a hollow body 6 formed as a helix, of say, four to l6 turns from top to bottom.
- an inert molded plastic such as a polyethylene, polypropylene or polyurethane having a memory and having the characteristic ,of transparency when molded into a finished object and includes a hollow body 6 formed as a helix, of say, four to l6 turns from top to bottom.
- the bottom is tapered as at 7, 7 by two diametrically opposed concave hollows that may serve as abutments for positioning the tube in a centrifuge and/or as abutments for the mechanical axial partial collapsing of the tube to discharge a portion of its contents into subsequently used processing apparatus.
- the bottom is closed by a membrane 8 and the top is provided with an axially extending hollow tip or cannula Q that extends into open communication with the interior of the body 6 and is, of course, sufficiently long to reach that portion of a patients body cavity from which a specimen is to be collected.
- the tip 9 may be covered by a friction cap (not shown) or other suitable closure to maintain same sterile until use, it being understood that the interior of the body 6 is already sterile due to its exposure to heat above sterilizing temperatures during formation of the tube.
- the body 6 is filled with an ultra-violet responsive dyed solution by immersing the tip 9 in a supply thereof and axially collapsing and releasing the body 6 to draw in a quantity of the solution by vacuum, after which the tip is closed.
- the tip is uncovered and inserted into the proper body cavity which becomes irrigated as the result of using the body 6 as a positive and negative acting syringe, thus collecting the required specimen for examination.
- the tube and its contents are transferred to a centrifuge where the solids are concentrated adjacent the bottom 8.
- the helices of the body 6 expedite the flow of solids toward the bottom during centrifuging or vortex mixing, thus assuring proper concentration of the solids in a substantially uniform distribution.
- ultra-violet light analysis of the concentrated solids may be readily effected without having to discharge any part thereof onto a slide or other receiver, simply by piercing the bottom membrane 8 with a sharp ended fibergpiic element or rod 10, capable of transmitting ultra-violetli'ght, so that its inner end is contained within the concentrated solids in the bottom of the tube and its outer end is exposed for operable connection to an external source of ultra-violet light which is transmitted to the solids via the rod 10.
- the transparency of the tube makes it readily possible to observe any ultraviolet effect on the contents of the tube and to visually compare that effect with an ultraviolet control or standard to determine, qualitatively, the presence or absence of cytological or histochemical abnormalities in the specimen.
- the solid fiber optic element 10 would be replaced by a hollow double-ended needle element 11 (see FIG. 3) so that the centrifuge tube also could be used as a positive acting syringe to discharge a portion of the specimen onto a slide or other receiver as an incident to such further testing.
- a molded centrifuge tube formed from that type of plastic that is transparent upon completion of mold ing and including; a hollow body shaped as a bellowsfolded helix, said body having a bottom closure provided with a needle-penetrable membrane and having a top provided with an axially extending hollow tip in open communication with the interior of said body, and an optical fiber element passing through said penetrable membrane with its inner end extending into the lower interior portion of said body and its outer end extending outwardly beyond said membrane for operable connection with an external source of light for transmission of said light into the interior of said body.
- centrifuge tube of claim 1 in which said body is formed from a plastic having a memory whereby to return to its normal configuration after having been physically distorted therefrom.
- centrifuge tube of claim 1 in which the bottom of said body is tapered by two diametrically opposed concave hollows that define abutments for properly positioning said tube during centrifuging or other mechanical operations.
- the centrifuge tube of claim 5 in which the material for said optical fiber element is selected from the group consisting of quartz, and a methyl methacrylate capable of transmitting ultra-violet light.
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Pathology (AREA)
- Veterinary Medicine (AREA)
- Analytical Chemistry (AREA)
- Heart & Thoracic Surgery (AREA)
- Medical Informatics (AREA)
- Molecular Biology (AREA)
- Surgery (AREA)
- Animal Behavior & Ethology (AREA)
- Engineering & Computer Science (AREA)
- Public Health (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Reproductive Health (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Gynecology & Obstetrics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Sampling And Sample Adjustment (AREA)
- Centrifugal Separators (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
This invention is directed to a centrifuge tube formed from a transparent plastic and provided at its bottom with a penetratable membrane through which an optical fiber element is passed upon completion of centrifuging of the contents to enable visual observation of the effect of light, particularly ultraviolet light, on the contents, when compared with a standard or control, to detect the presence or absence of a predetermined type of foreign matter in the centrifuged contents of the tube. The optical fiber element may be formed from quartz or from such a material as a methyl methacrylate capable of transmitting ultra-violet light.
Description
Bifi fid srz KR 397525146 SEARCH ROOM United Statw V [111 3,752,146 Kline SUBSTITUTE FOR MISSING XR [45] g- 1973 [54] CENTRIFUGE TUBE FOR ULTRA-VIOLET 3,525,803 8/1970 Smart 128/2 A ANALYSIS 3,570,662 3/1971 Polyak [28/232 [75] Inventor: William M. Kline, Gloversville, N.Y. FOREIGN PATENTS OR APPLICATIONS [73] Assignee, Medical Evaluation Devices & 1,112,715 5/1968 Great Britain 128/2 F Instruments Co G1 11 rp overs 6 Primary Examiner-Kyle L. Howell Attorney-Thomas E. Tate [22] Filed: Dec. 13, 1971 [21] Appl. N0.: 207,192 ABSTRACT This invention is directed to a centrifuge tube formed 521 US. Cl. 128/2 A, 23/259 128/2 F a transparent Plastic and Pmvided at its 128 /2 with a penetratable membrane through which an opti- 511 1nt.Cl A61b 10/00 cal fiber element is Passed wmpletion centri- [58] Field 0: Search 128/2 F, 2 R 2 G fusing 0f the enable visual bservatin 128/2 Ay DIG. 5 275 27 23 259. 3 39 the effect Of light, particularly ultra-violet light, 011 the 51 2 4 contents, when compared with a standard or control, to detect the presence or absence of a predetermined type [56] References Cited of foreign matter in the centrifuged contents of the tube. The optical fiber element may be formed from UNITED STATES PATENTS quartz or from such a material as a methyl methacry- 3,712,295 1/1973 Kline 128/2 F late capable of transmitting ultrawiolet light 3,480,398 11/1969 Hamilton 23/259 X 3,327,! 19 6/1967 Kamentsky 128/2 A 8 Claims, 3 Drawing Figures CENTRIFUGE TUBE FOR ULTRA-VIOLET ANALYSIS THE INVENTION This invention relates to new and useful improvements in laboratory equipment and particularly seeks to provide a novel molded centrifuge tube formed from that type of plastic that is transparent upon completion of molding so that the contents of the tube, during use thereof, may be subjected to ultra-violet light analysis without having to be transferred to a separate container or receiving slide.
This invention is an improvement over that disclosed and claimed in my copending US. Pat. application Ser. No. 60,354, filed Aug. 3, 1970, now US. Pat. No. 3712295, for Centrifuge Tube and also is an improvement over that disclosed and claimed in my continuation-in-part copending US. Pat. application Ser. No. 207447 filed Dec. 13, 1971, for Multipurpose Centrifuge Tube.
In certain diagnostic procedures, such as the PAP test for cancer of the cervix, the area to be tested generally is irrigated with a pre-or post-dyed saline or other solution which is then withdrawn and centrifuged to separate the solids for subsequent reagent or other analysis. Such an analysis may include subjecting the dyed specimen solids to exposure to ultra-violet light to visually indicate the qualitative presence or absence of cytological and histo-chemical abnormalities in the specimen by color changes as compared to a control. Obviously the dyes are selected to be effective for the precise type of ultra-violet indication required. That is to say, one dye or a class of dyes may give an ultraviolet response to cancerous matter, for example, while another dye or class of dyes may give an ultra-violet response to another type of detectable matter in the specimen.
A centrifuge tube constructed in accordance with this invention enables such procedures to be greatly simplified in that the same tube may be used to collect the specimen, centrifuge the specimen and subject the specimen to ultra-violet light analysis without having to transfer any portion of the centrifuged specimen to a slide or other external receiver.
The effectiveness of the centrifuge tube of this invention stems from the fact that it is transparent and is pro vided at its bottom with a membrane closure that can be penetrated by a sharp ended optical fiber element that is capable of transmitting ultra-violet light from an external source into the bottom portion of the tube upon completion of centrifuging.
Such an optical fiber element may be formed from quartz or from any other material capable of transmitting ultra-violet light, such as a methyl methacrylate of the type commercially available from Imperial Chemical Industries under the Trademark DlAKON, or its equivalent.
Therefore, an object of this invention is to provide a transparent centrifuge tube formed from asmooth, inert, flexible plastic having a memory, and having a syringe tip at its top and a sealed bottom that is penetrable by a sharp ended optical fiber element capable of transmitting ultra-violet light to the interior of said tube.
Another object of this invention is to provide a centrifuge tube of the character stated in which the body is formed as a bellows-folded helix to direct the flow of solid particles to the bottom when centrifuged.
Another object of this invention is to provide a centrifuge tube of the character stated in which said optical fiber element is in the form of a double tipped hollow needle to enable said tube to be used as a positive acting syringe to discharge the concentrated solids for subsequent examination following completion of centrifuging and ultra-violet light analysis of the contents.
A further object of this invention is to provide a centrifuge tube of the character stated in which the sealed bottom is tapered by two diametrically opposed concave hollows that may serve as abutments for the positioning of the tube in a centrifuge and/or as abutments for the mechanical axial partial collapsing of the tube in subsequently used processing apparatus.
A further object of this invention is to provide a centrifuge tube of the character stated that is simple in design, rugged in construction and economical to manufacture.
With these and other objects, the nature of which will be apparent, the invention will be more fully understood by reference to the drawings, the accompanying detailed description and the appended claims.
In the drawings:
FIG. 1 is a side elevation of a centrifuge tube constructed in accordance with this invention;
FIG. 2 is a fragmentary section of the bottom thereof showing the insertion of a sharp ended optical fiber element through the bottom for ultra-violet light analysis of the centrifuged contents; and
FIG. 3 is a detail elevation, partly in section, showing a modified form of optical fiber element to permit the tube to be used as a syringe for discharge of at least a portion of the contents.
Referring to the drawings in detail the invention, as illustrated, is embodied in a transparent centrifuge tube generally indicated 5 formed from an inert molded plastic such as a polyethylene, polypropylene or polyurethane having a memory and having the characteristic ,of transparency when molded into a finished object and includes a hollow body 6 formed as a helix, of say, four to l6 turns from top to bottom.
The bottom is tapered as at 7, 7 by two diametrically opposed concave hollows that may serve as abutments for positioning the tube in a centrifuge and/or as abutments for the mechanical axial partial collapsing of the tube to discharge a portion of its contents into subsequently used processing apparatus. The bottom is closed by a membrane 8 and the top is provided with an axially extending hollow tip or cannula Q that extends into open communication with the interior of the body 6 and is, of course, sufficiently long to reach that portion of a patients body cavity from which a specimen is to be collected. The tip 9 may be covered by a friction cap (not shown) or other suitable closure to maintain same sterile until use, it being understood that the interior of the body 6 is already sterile due to its exposure to heat above sterilizing temperatures during formation of the tube.
In preparation for use in diagnostic tests, such as the above mentioned PAP test, the body 6 is filled with an ultra-violet responsive dyed solution by immersing the tip 9 in a supply thereof and axially collapsing and releasing the body 6 to draw in a quantity of the solution by vacuum, after which the tip is closed.
At the point of use, the tip is uncovered and inserted into the proper body cavity which becomes irrigated as the result of using the body 6 as a positive and negative acting syringe, thus collecting the required specimen for examination. After the specimen has been collected the tube and its contents are transferred to a centrifuge where the solids are concentrated adjacent the bottom 8. The helices of the body 6 expedite the flow of solids toward the bottom during centrifuging or vortex mixing, thus assuring proper concentration of the solids in a substantially uniform distribution.
At this stage, ultra-violet light analysis of the concentrated solids may be readily effected without having to discharge any part thereof onto a slide or other receiver, simply by piercing the bottom membrane 8 with a sharp ended fibergpiic element or rod 10, capable of transmitting ultra-violetli'ght, so that its inner end is contained within the concentrated solids in the bottom of the tube and its outer end is exposed for operable connection to an external source of ultra-violet light which is transmitted to the solids via the rod 10. The transparency of the tube makes it readily possible to observe any ultraviolet effect on the contents of the tube and to visually compare that effect with an ultraviolet control or standard to determine, qualitatively, the presence or absence of cytological or histochemical abnormalities in the specimen.
lf further testing of the concentrated solids is required beyond that obtained by ultra-violet light analysis as described above, the solid fiber optic element 10 would be replaced by a hollow double-ended needle element 11 (see FIG. 3) so that the centrifuge tube also could be used as a positive acting syringe to discharge a portion of the specimen onto a slide or other receiver as an incident to such further testing.
It is of course to be understood that variations in arrangements, changes in materials and proportions of parts may be made within the scope of the appended claims.
I claim;
1. A molded centrifuge tube formed from that type of plastic that is transparent upon completion of mold ing and including; a hollow body shaped as a bellowsfolded helix, said body having a bottom closure provided with a needle-penetrable membrane and having a top provided with an axially extending hollow tip in open communication with the interior of said body, and an optical fiber element passing through said penetrable membrane with its inner end extending into the lower interior portion of said body and its outer end extending outwardly beyond said membrane for operable connection with an external source of light for transmission of said light into the interior of said body.
2. The centrifuge tube of claim 1 in which said body is formed from a plastic having a memory whereby to return to its normal configuration after having been physically distorted therefrom.
3. The centrifuge tube of claim 2 in which said plastic is selected from the group consisting of polyethylene, polypropylene and polyurethane.
4. The centrifuge tube of claim 1 in which the bottom of said body is tapered by two diametrically opposed concave hollows that define abutments for properly positioning said tube during centrifuging or other mechanical operations.
5. The centrifuge tube of claim 1 in which said optical fiber element is made from a material capable of transmitting ultra-violet light.
6. The centrifuge tube of claim 5 in which the material for said optical fiber element is selected from the group consisting of quartz, and a methyl methacrylate capable of transmitting ultra-violet light.
7. The centrifuge tube of claim 5 in which said optical fiber element is solid.
8. The centrifuge tube of claim 5 in which said optical fiber element is in the form of a hollow needle.
Claims (8)
1. A molded centrifuge tube formed from that type of plastic that is transparent upon completion of molding and including; a hollow body shaped as a bellows-folded helix, said body having a bottom closure provided with a needle-penetrable membrane and having a top provided with an axially extending hollow tip in open communication with the interior of said body, and an optical fiber element passing through said penetrable membrane with its inner end extending into the lower interior portion of said body and its outer end extending outwardly beyond said membrane for operable connection with an external source of light for transmission of said light into the interior of said body.
2. The centrifuge tube of claim 1 in which said body is formed from a plastic having a memory whereby to return to its normal configuration after having been physically distorted therefrom.
3. The centrifuge tube of claim 2 in which said plastic is selected from the group consisting of polyethylene, polypropylene and polyurethane.
4. The centrifuge tube of claim 1 in which the bottom of said body is tapered by two diametrically opposed concave hollows that define abutments for properly positioning said tube during centrifuging or other mechanical operations.
5. The centrifuge tube of claim 1 in which said optical fiber element is made from a material capable of transmitting ultra-violet light.
6. The centrifuge tube of claim 5 in which the material for said optical fiber element is selected from the group consisting of quartz, and a methyl methacrylate capable of transmitting ultra-violet light.
7. The centrifuge tube of claim 5 in which said optical fiber element is solid.
8. The centrifuge tube of claim 5 in which said optical fiber element is in the form of a hollow needle.
Priority Applications (16)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US00060354A US3712295A (en) | 1970-08-03 | 1970-08-03 | Centrifuge tube |
CH917871A CH519905A (en) | 1970-08-03 | 1971-06-23 | Centrifuge tube |
CA116877A CA931125A (en) | 1970-08-03 | 1971-06-28 | Centrifuge tube |
JP46048886A JPS5019791B1 (en) | 1970-08-03 | 1971-07-05 | |
FR7125940A FR2101739A5 (en) | 1970-08-03 | 1971-07-15 | |
GB3572671A GB1331140A (en) | 1970-08-03 | 1971-07-29 | Centrifuge tube |
DE2138180A DE2138180C3 (en) | 1970-08-03 | 1971-07-30 | Plastic centrifuge tube |
SE7109937A SE376724B (en) | 1970-08-03 | 1971-08-03 | |
US00207192A US3752146A (en) | 1970-08-03 | 1971-12-13 | Centrifuge tube for ultra-violet analysis |
US00207447A US3796542A (en) | 1970-08-03 | 1971-12-13 | Use of multi-purpose centrifuge tube in biological analysis |
CH1626472A CH543740A (en) | 1970-08-03 | 1972-11-08 | Method for analyzing an organic sample by irradiation with light and a centrifuge tube for carrying out the method |
CA156,025A CA965627A (en) | 1970-08-03 | 1972-11-08 | Centrifuge tube for ultra-violet analysis |
DE19722260964 DE2260964B2 (en) | 1970-08-03 | 1972-12-13 | CENTRIFUGATION TUBE MADE OF PLASTIC |
GB5738272A GB1384296A (en) | 1970-08-03 | 1972-12-13 | Centrifuge tube for ultra-violet analysis |
FR7244357A FR2163571B2 (en) | 1970-08-03 | 1972-12-13 | |
US00362906A US3838970A (en) | 1970-08-03 | 1973-05-23 | Centrifuge tube for ultra-violet analysis |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US00060354A US3712295A (en) | 1970-08-03 | 1970-08-03 | Centrifuge tube |
US00207192A US3752146A (en) | 1970-08-03 | 1971-12-13 | Centrifuge tube for ultra-violet analysis |
Publications (1)
Publication Number | Publication Date |
---|---|
US3752146A true US3752146A (en) | 1973-08-14 |
Family
ID=41432893
Family Applications (4)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US00060354A Expired - Lifetime US3712295A (en) | 1970-08-03 | 1970-08-03 | Centrifuge tube |
US00207192A Expired - Lifetime US3752146A (en) | 1970-08-03 | 1971-12-13 | Centrifuge tube for ultra-violet analysis |
US00207447A Expired - Lifetime US3796542A (en) | 1970-08-03 | 1971-12-13 | Use of multi-purpose centrifuge tube in biological analysis |
US00362906A Expired - Lifetime US3838970A (en) | 1970-08-03 | 1973-05-23 | Centrifuge tube for ultra-violet analysis |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US00060354A Expired - Lifetime US3712295A (en) | 1970-08-03 | 1970-08-03 | Centrifuge tube |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US00207447A Expired - Lifetime US3796542A (en) | 1970-08-03 | 1971-12-13 | Use of multi-purpose centrifuge tube in biological analysis |
US00362906A Expired - Lifetime US3838970A (en) | 1970-08-03 | 1973-05-23 | Centrifuge tube for ultra-violet analysis |
Country Status (8)
Country | Link |
---|---|
US (4) | US3712295A (en) |
JP (1) | JPS5019791B1 (en) |
CA (2) | CA931125A (en) |
CH (2) | CH519905A (en) |
DE (2) | DE2138180C3 (en) |
FR (2) | FR2101739A5 (en) |
GB (2) | GB1331140A (en) |
SE (1) | SE376724B (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4317615A (en) * | 1978-07-18 | 1982-03-02 | Espe Fabrik Pharmazeurischer Preparate Gmbh | Protective case for a quartz-rod optical wave guide |
US5342329A (en) * | 1991-10-11 | 1994-08-30 | Inmed Ltda. | Portable disposable device for post-surgical suction |
WO1998053348A1 (en) * | 1997-05-21 | 1998-11-26 | Mcgaffigan Thomas H | Optical light pipes with laser light appearance |
US6031958A (en) * | 1997-05-21 | 2000-02-29 | Mcgaffigan; Thomas H. | Optical light pipes with laser light appearance |
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Publication number | Priority date | Publication date | Assignee | Title |
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US3817239A (en) * | 1972-05-12 | 1974-06-18 | D Kuntz | Urine monitor |
US4022191A (en) * | 1976-06-04 | 1977-05-10 | Khosrow Jamshidi | Biopsy needle guard and guide |
US4187861A (en) * | 1978-02-21 | 1980-02-12 | Heffernan Bart T | Blood sample handling apparatus and method |
GB2021982B (en) * | 1978-06-05 | 1982-12-22 | Beckman Instruments Inc | Integral one piece centrifuge tube |
US4167955A (en) * | 1978-06-05 | 1979-09-18 | Beckman Instruments, Inc. | Layering a liquid sample onto centrifuge tube liquid contents |
US4250893A (en) * | 1979-02-21 | 1981-02-17 | American Hospital Supply Corporation | Sample collection device |
DE3001409A1 (en) * | 1980-01-16 | 1981-08-06 | Rostislav Dr. 7910 Neu-Ulm Caletka | Conical connection between syringe cylinder and outlet - for sepn. of immiscible liquids |
GB2181055A (en) * | 1984-11-20 | 1987-04-15 | David John Colgate | Disposable syringe |
US4610171A (en) * | 1985-04-26 | 1986-09-09 | Nason Frederic L | Urinanalysis vial |
EP0238609A1 (en) * | 1985-10-01 | 1987-09-30 | Axipac Limited | Disposable syringe |
GB2206794A (en) * | 1987-07-14 | 1989-01-18 | Richard Kiteley Power | Syringe |
US4867172A (en) * | 1988-02-23 | 1989-09-19 | Habley Medical Technology Corporation | Collapsible blood collector |
FR2631447B1 (en) * | 1988-05-11 | 1992-12-04 | Shell Int Research | INJECTION SYSTEM FOR ANALYZER |
DE3905380C1 (en) * | 1989-02-22 | 1990-07-05 | Peter Dr.Med. 7140 Ludwigsburg De Schulz | Removal kit for samples |
US5102398A (en) * | 1990-09-18 | 1992-04-07 | Farris Barry L | Plungerless syringe |
DE69117464T2 (en) * | 1990-09-18 | 1996-10-10 | Barry Farris | Pistonless syringe |
US5309924A (en) * | 1992-04-29 | 1994-05-10 | Peabody Alan M | Spill-proof blood collection device |
US5308322A (en) * | 1993-04-19 | 1994-05-03 | Tennican Patrick O | Central venous catheter access system |
US5538506A (en) * | 1993-11-03 | 1996-07-23 | Farris; Barry | Prefilled fluid syringe |
CA2145868A1 (en) * | 1994-04-12 | 1995-10-13 | Osamu Tsukada | Accordion container for chemical |
JPH09141135A (en) * | 1995-11-17 | 1997-06-03 | Blue Jiyuuji:Kk | Sample tube for centrifugal separation |
US5804744A (en) * | 1996-09-30 | 1998-09-08 | Chemtrace | Apparatus for obtaining, storing and transporting liquid samples and methods for making and using same |
USD432647S (en) * | 1999-02-25 | 2000-10-24 | Barry Farris | No reflux syringe |
US6296150B1 (en) | 1999-02-25 | 2001-10-02 | Barry Farris | Medicinal dosing apparatus and method |
USD432646S (en) * | 1999-02-26 | 2000-10-24 | Barry Farris | Syringe with air trap |
DE19922285A1 (en) * | 1999-05-14 | 2000-11-16 | Febit Ferrarius Biotech Gmbh | Sample vessel used for handling liquids, powders and granules has two front ends with a casing between the ends to limit a sample vessel chamber |
US6367659B1 (en) | 2000-08-24 | 2002-04-09 | Concept Workshop Worldwide Llc | Telescopic liquid dispenser |
US6773417B2 (en) | 2001-07-06 | 2004-08-10 | Ispg, Inc. | Epidural space locating device |
WO2003019131A2 (en) * | 2001-08-29 | 2003-03-06 | Hexal Pharma Gmbh | Method and device for preparing a sample of biological origin in order to determine at least one constituent contained therein |
WO2006076819A1 (en) * | 2005-01-21 | 2006-07-27 | Medic Tools Ag | One-way fractionating device |
AU2010235996B2 (en) * | 2009-10-26 | 2014-04-17 | Asp Rights Management Pty Ltd | A "Sharps" Container |
GB201508752D0 (en) * | 2015-05-21 | 2015-07-01 | Mason Christopher And Veraitch Farlan S | Cell culture device, system and methods of use thereof |
CN106475164B (en) * | 2016-10-24 | 2019-01-11 | 深圳市孔雀生物科技有限公司 | A kind of combined centrifugal device |
CN107929825B (en) * | 2017-12-05 | 2020-04-21 | 新乡医学院 | Drainage tube and drainage device |
CN113877650B (en) * | 2021-11-10 | 2022-11-25 | 美东汇成生命科技(昆山)有限公司 | Centrifugal tube |
Family Cites Families (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA773863A (en) * | 1967-12-19 | J. Davis Hugh | Pipette | |
US2353153A (en) * | 1942-08-05 | 1944-07-11 | Clyde B Ferrel | Clinical irrigating device |
US2542456A (en) * | 1947-08-26 | 1951-02-20 | Laval Separator Co De | Standardizing centrifugally separable product |
US2771071A (en) * | 1953-09-12 | 1956-11-20 | Mann Gerhard | Ampulla structure |
US2964993A (en) * | 1958-08-06 | 1960-12-20 | Honeywell Regulator Co | Analyzing apparatus |
FR1248279A (en) * | 1960-02-04 | 1960-10-31 | Ampoule, in particular for pharmaceutical use | |
US3175553A (en) * | 1960-03-07 | 1965-03-30 | Memorial Hospital | Urine test kit |
US3181529A (en) * | 1962-07-30 | 1965-05-04 | Edgar H Wilburn | Valved body-fluid sampling tubes |
GB1095316A (en) * | 1963-08-08 | 1967-12-13 | Arthur Bane | Improvements in ampoules for surgical hypodermic syringes |
FR1426127A (en) * | 1964-05-12 | 1966-01-28 | Dept De La Vienne | Venous blood collection equipment |
GB1112715A (en) * | 1965-07-16 | 1968-05-08 | Lancelot Richard Rowett | Disposable urine specimen tube and cap therefor |
GB1175184A (en) * | 1966-02-08 | 1969-12-23 | Arthur Bane | Improvements in Syringe Ampoules. |
FR1493491A (en) * | 1966-05-13 | 1967-09-01 | Moser & Cie Soc Nouv | Applicator-doser for medical use |
US3532470A (en) * | 1968-01-22 | 1970-10-06 | Beckman Instruments Inc | Sample holder with centrifugation means |
US3727066A (en) * | 1968-02-16 | 1973-04-10 | Baxter Laboratories Inc | Probe photometer with fluid sensing device |
US3557788A (en) * | 1968-03-08 | 1971-01-26 | Betty J Swartz | Disposable syringe |
US3570662A (en) * | 1969-02-07 | 1971-03-16 | Jack Polyak | Disposable squeeze tube with foldable nozzle |
FR2036769A1 (en) * | 1969-03-26 | 1970-12-31 | Dassault Electronique | |
US3582283A (en) * | 1970-03-25 | 1971-06-01 | Xerox Corp | Chemical package |
US3790791A (en) * | 1972-07-20 | 1974-02-05 | Bunker Ramo | Optoelectronic cable assembly |
-
1970
- 1970-08-03 US US00060354A patent/US3712295A/en not_active Expired - Lifetime
-
1971
- 1971-06-23 CH CH917871A patent/CH519905A/en not_active IP Right Cessation
- 1971-06-28 CA CA116877A patent/CA931125A/en not_active Expired
- 1971-07-05 JP JP46048886A patent/JPS5019791B1/ja active Pending
- 1971-07-15 FR FR7125940A patent/FR2101739A5/fr not_active Expired
- 1971-07-29 GB GB3572671A patent/GB1331140A/en not_active Expired
- 1971-07-30 DE DE2138180A patent/DE2138180C3/en not_active Expired
- 1971-08-03 SE SE7109937A patent/SE376724B/xx unknown
- 1971-12-13 US US00207192A patent/US3752146A/en not_active Expired - Lifetime
- 1971-12-13 US US00207447A patent/US3796542A/en not_active Expired - Lifetime
-
1972
- 1972-11-08 CH CH1626472A patent/CH543740A/en not_active IP Right Cessation
- 1972-11-08 CA CA156,025A patent/CA965627A/en not_active Expired
- 1972-12-13 DE DE19722260964 patent/DE2260964B2/en active Granted
- 1972-12-13 GB GB5738272A patent/GB1384296A/en not_active Expired
- 1972-12-13 FR FR7244357A patent/FR2163571B2/fr not_active Expired
-
1973
- 1973-05-23 US US00362906A patent/US3838970A/en not_active Expired - Lifetime
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4317615A (en) * | 1978-07-18 | 1982-03-02 | Espe Fabrik Pharmazeurischer Preparate Gmbh | Protective case for a quartz-rod optical wave guide |
US5342329A (en) * | 1991-10-11 | 1994-08-30 | Inmed Ltda. | Portable disposable device for post-surgical suction |
WO1998053348A1 (en) * | 1997-05-21 | 1998-11-26 | Mcgaffigan Thomas H | Optical light pipes with laser light appearance |
US6031958A (en) * | 1997-05-21 | 2000-02-29 | Mcgaffigan; Thomas H. | Optical light pipes with laser light appearance |
US6160948A (en) * | 1997-05-21 | 2000-12-12 | Mcgaffigan; Thomas H. | Optical light pipes with laser light appearance |
US6337946B1 (en) | 1997-05-21 | 2002-01-08 | Mcgaffigan Thomas H. | Optical light pipes with laser light appearance |
Also Published As
Publication number | Publication date |
---|---|
DE2138180C3 (en) | 1975-01-16 |
DE2138180A1 (en) | 1972-02-10 |
CH519905A (en) | 1972-03-15 |
GB1384296A (en) | 1975-02-19 |
CH543740A (en) | 1973-10-31 |
SE376724B (en) | 1975-06-09 |
FR2101739A5 (en) | 1972-03-31 |
CA931125A (en) | 1973-07-31 |
JPS5019791B1 (en) | 1975-07-09 |
GB1331140A (en) | 1973-09-19 |
DE2260964A1 (en) | 1973-06-28 |
DE2260964B2 (en) | 1976-06-16 |
US3796542A (en) | 1974-03-12 |
CA965627A (en) | 1975-04-08 |
FR2163571A2 (en) | 1973-07-27 |
US3712295A (en) | 1973-01-23 |
DE2138180B2 (en) | 1974-05-30 |
US3838970A (en) | 1974-10-01 |
FR2163571B2 (en) | 1977-04-08 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
AS | Assignment |
Owner name: MEDICAL EVALUATION DEVICES & INSTRUMENTS CORP. 6 D Free format text: RELEASE OF LEVY BY IRS;ASSIGNOR:INTERNAL REVENUE SERVICE;REEL/FRAME:003942/0059 Effective date: 19800729 |