US3747843A - Continuous flow zonal rotor - Google Patents

Continuous flow zonal rotor Download PDF

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Publication number
US3747843A
US3747843A US00132663A US3747843DA US3747843A US 3747843 A US3747843 A US 3747843A US 00132663 A US00132663 A US 00132663A US 3747843D A US3747843D A US 3747843DA US 3747843 A US3747843 A US 3747843A
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United States
Prior art keywords
sample
gradient
zone
rotor
core
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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US00132663A
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English (en)
Inventor
J Joyce
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Electric Corp
Thermo IEC Inc
BNP Paribas New York
Original Assignee
Damon Corp
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Assigned to BANQUE PARIBAS, THE EQUITABLE TOWER 787 SEVENTH AVENUE NEW YORK, NY 100019 reassignment BANQUE PARIBAS, THE EQUITABLE TOWER 787 SEVENTH AVENUE NEW YORK, NY 100019 ASSIGNMENT OF ASSIGNORS INTEREST. Assignors: INTERNATIONAL EQUIPMENT COMPANY
Assigned to INTERNATIONAL EQUIPMENT COMPANY, A CORP OF DE reassignment INTERNATIONAL EQUIPMENT COMPANY, A CORP OF DE ASSIGNMENT OF ASSIGNORS INTEREST. Assignors: DAMON CORPORATION, A CORP OF DE
Assigned to MITSUBISHI DENKI KABUSHIKI KAISHA reassignment MITSUBISHI DENKI KABUSHIKI KAISHA ASSIGNMENT OF ASSIGNORS INTEREST. Assignors: IWATA, TOSHIO, MATSUOKA, ATSUKO
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Expired - Lifetime legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0442—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0442—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
    • B04B2005/045—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation having annular separation channels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0442—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
    • B04B2005/0464—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation with hollow or massive core in centrifuge bowl

Definitions

  • ABSTRACT A method and apparatus for continuous flow centrifugation wherein a sample is introduced through the top surface of a rotor core into a sample zone adjacent a gradient zone so that the sample passes substantially 360 and a sample gradient is formed in said gradient zone.
  • the spent sample is removed through the bottom surface of said rotor core, and the sample gradient is removed after separation by introducing a liquid gradient through thetop surface of the rotor core to the gradient zone to displace the sample gradient radially inward.
  • the sample gradient is passed out of the core while the rotor is spinning through a passage at the core axis at a reduced pressure in the passage. Since only one liquid stream enters or exits at or near the core axis, the possibility of sample product contamination is eliminated.
  • a sample to be separated is pumped continuously into a rotating centrifuge and into a liquid gradient zone.
  • the gradient zone is formed so that the liquid gradient density, which increases with its radial position away from the rotor center, is regulated to separate sample strata therein.
  • sample separation can be effected at any desired radial position. Generally the desired radial position is chosen for ease of sample recovery.
  • the density gradient of the gradient liquid usually is a concentration gradient of a relatively non-diffusing suitable solute such as salt or sucrose.
  • the sample liquid is pumped continuously into a zone radially intermediate the gradient liquid and the rotor and is caused to circulate around all or a portion of the rotor circumference.
  • the gradient functions to entrap particles sedimenting from the sample liquid without further radial movement of the particles at that location where their density equals that of the gradient. The travel of particles of greater or lesser density is varied accordingly.
  • Some zonal rotors have separate sectors and the sample is divided into streams flowing separately through its sectors. Such sectors are of relatively large volume, each sector typically representing 25 percent of the capacity of the rotor.
  • a zonal rotor is available having a particle collecting sector that extends substantially 360 to permit the sample to travel the same distance within the. rotor. The latter centrifuge is preferable since it provides more effective sample separation. This centrifuge is described in a copending application to John E. Joyce entitled “Rotors and Rotor Cores for Continuous Flow Centrifuges", Ser. No. 129,055 filed Mar. 29, 1971.
  • a centrifuge rotor is provided with two circular grooves in its top surface, each groove being adjacent the other and radially separated from each other.
  • First and second passages through the rotor connect the grooves with either a sample zone or a gradient zone located between the peripheral rotor surface and the housing.
  • the first passages have an outlet radially outward from the second passage outlets.
  • a third passage has an inlet at sample zone and an outlet in the bottom surface of the rotor to remove the spent sample.
  • a fourth passage connects the sample zone with an outlet conduit at the rotor axis which can be connected with a means for reducing pressure below atmospheric.
  • the fourth passage has a seal with a rotating section and a stationary section that permits sample removal from the spinning rotor.
  • the reduced pressure employed is defined by the equation:
  • d(lbs/cu. in.) is the density of the fluid being removed
  • N(rpm) is the speed of the rotor
  • r(in) is the radial position of sample drain outlet
  • B is a proportionality constant equal to 2.841 X 10"".
  • FIG. 1 is a top view of a centrifuge rotor.
  • FIG. 2 is an elevation cross-sectional view of the apparatus for'removing separated sample.
  • FIG. 3 is a partial cross-section view taken along line 3 of FIG. 1.
  • FIG. 4 is a cross-sectional view taken along line 4 of FIG. I.
  • FIG. 5 is a cross-sectional view taken along line 5 of FIG. 1.
  • FIG. 6 is a partial cross-sectional view taken along Line 6 of FIG. 1.
  • FIG. 7 is a partial cross-sectional view taken along Line 7 of FIG. 1.
  • the centrifuge generally indicated at 1 comprises a rotor housing 2 and a rotor 3 having a frusto-conical shape.
  • the rotor housing 2 has a lower axial socket 8 to receive a motor drive (not shown) and an upper axial socket 9 to receive a sealed sample recovery system described hereafter with reference to FIG. 2.
  • the rotor 3 is provided with a plurality of fluid passages for the introduction and exit of liquid gradient and fluid sample in a continuous cycle so that the sample travels about 360 around the rotor 3.
  • the large arrows indicate the direction and path of sample flow.
  • the liquid gradient and introduction of sample is accomplished as follows: While the rotor 3 is spinning, liquid gradient is introduced into circular groove 10 by placing the outlet of a tube adjacent to or into the groove 10 for delivery of fluid thereto.
  • the liquid gradient passes from groove 10 through a plurality of passageways 11 that terminate at the ends of fins 12a, 12b, 12c and 22 which are positioned adjacent the rotor housing 2.
  • Introduction of liquid gradient into groove 10 and passageways 11 is continued until the desired size of the gradient zone 13A is effected.
  • the gradient zone 13A extends from the inner surface of rotor housing 2 to the radially outermost point of rotor 3.
  • sample to be separated is introduced into circular groove 15 which is radially inward from groove 10 and is connected with sample delivery passage 16 having an outlet 17 that is positioned radially inward from the gradient zone 13A.
  • the fluid sample is constantly fed to groove 15 and into passageway 16 so that there is established inside the rotor 3, while it is spinning, a sample zone 18 that travels clockwise around the rotor 3 substantially 360 until it reaches drain passageway 19 and outlet 20 through which spent sample is removed from the rotor 3.
  • the sealing arrangement shown in FIG. 2 Prior to introducing the liquid gradient after sample separation and during the introduction of the new liquid gradient, the sealing arrangement shown in FIG. 2 is positioned so that fluid passing from passage 26 through outlet 27 can pass into inlet 28 of a sample removal tube 29.
  • the sealing arrangement shown in FIG. 2 comprises generally two sections; a rotating seal 30 and a stationary seal 31. The rotating seal 30 and stationary seal 31 are maintained in close contact by means of spring 32.
  • the rotating seal housing 33 is held in the main seal housing 34 by means of ball bearings 35 and the seal housing 33 is moved into and out of rotor 3 by means of lever 36 which is pivoted around pin 37 and bears against the top surface 38 of seal housing 34.
  • the lever 36 moves against the action of spring 40.
  • the extension 30a sealed to rotating seal 30 fits into an arbor 44 which in turn, is fit into socket 9 and sealed therein by means of O ring 45.
  • the extension 30a is sealed in arbor 44 by means of O ring 46.
  • arbor 44, extension 30a, seal 30 seal housing 33 hearing 35 also rotate while seal 31, spring 32, housing 34, tube 29, spring 40 and lever 36 do not rotate.
  • the main seal housing 34 When the rotor housing 2 is rotated, arbor 44, extension 30a, seal 30 seal housing 33 hearing 35 also rotate while seal 31, spring 32, housing 34, tube 29, spring 40 and lever 36 do not rotate.
  • the main seal housing 34 is held in the main seal housing 34 by means of ball bearings 35 and the seal housing 33 is moved into and out of
  • sample recovery is effected by means of pump 50 which reduces the pressure in collection chamber 51; tube 29, and passage 26 when valve 52 is open and valves 53 and 54 are closed thereby causing gradual withdrawal of the liquid gradient and the sample strata therein while liquid gradient is being introduced into passage 11, as described above.
  • Sample recovery is effected 'while the rotor housing 2 is spinning at moderate speeds usually about 1000 to 2000 rpm so that the sample strata remain separate in the liquid gradient during recovery.
  • the sample strata are recovered sequentially, with the least dense stratium being recovered first. After the desired sample portion is collected in collection chamber 51, valve 52 is closed and valves 53 and 54 are open so that the sample can be recovered through conduit 55.
  • the drain outlet for spent sample is radially outward from the radially outer most groove to obtain the desired liquid flow.
  • the groove adapted to receive liquid gradient during sample recovery be located radially outward from the groove adapted to receive the sample to attain desired liquid flow.
  • the grooves be inclined or shaped so the lower portion thereof extends radially further outward than the upper portion to reduce spillage from the grooves onto the top surface of the rotor.
  • the outlet for the liquid gradient passage extend to the inner surface of the housing to provide ease in gradient zone formation and separated sample removal. in this respect, the liquid gradient passage need not extend below the openings 21 in fins 12a, 12b and 12c.
  • tube through the openings 21 can be employed to connect liquid gradient passages in the rotor body and the radially outermost portion of the f'ins.
  • the rotor can be segmented by closing the openings 21 in fins 12a, 12b and 120 and additional sample inlet passages and drains are provided for each rotor segment so that the sample travels only about 90 through the rotor.
  • this embodiment is not preferred since sample separation therein is less complete for a given sample flow rate.
  • more or less sample outlet and gradient inlet passages can be employed to attain equivalent sample separation and recovery.
  • any sealing arrangement can be employed so long as it is effective in providing passage for one liquid stream and to retain adequate sealing between mating rotatable and stationary sections.
  • a method for forming a sample gradient which comprises forming a gradient zone in a spinning centrifuge, flowing a sample through the top surface of a rotor core into a sample zone adjacent the gradient zone so that the sample passes substantially 360 and a sample gradient is formed in said gradient zone, removing spent sample through the bottom surface of said rotor core, and removing the sample gradient by introducing liquid gradient through the top surface of the rotor core to the gradient zone to displace said sample gradient radially inward and passing said sample gradient out of said core while the rotor is spinning through a passage at the axis of the core at a subatmospheric pressure in said passage.
  • a continuous flow centrifuge rotor comprising a rotor core having means for introducing two separate streams of liquid through circular grooves in the top surface of the rotor so that one stream can be directed into a sample zone and the other stream can be directed into a gradient zone, a means for removing spent sample from said sample zone through an outlet on the bottom surface of said rotor and means for removing separated sample from said rotor through a passage at the axis of said rotor while the rotor is spinning.
  • the centrifuge of claim 2 having means for flowing a sample stream through said sample zone substantially 360 around said rotor core.
  • a continuous flow centrifuge comprising a rotor housing a rotor core within said housing and spaced from said housing to define a gradient zone adjacent said housing and a sample zone between said rotor and said gradient zone, said rotor core having a top surface, a bottom surface and a peripheral surface, said top surface having'a first circular groove and a second circular groove radially spaced from said first circular groove each groove adapted to receive means for delivering a liquid, at least onepassage connecting said first groove with said gradient zone andat least one passage connecting said second groove with said sample zone at least one drain passage in said core connecting said sample zone and said bottom surface, said drain passage having an outlet radially intermediate the radially outermost groove and the peripheral surface, at least one sample recovery passage in said core connecting the sample zone and an axial passage at the axis of said core and said axial passage having means to recover separated sample at subatmospheric pressure in said axial passage pressure while said rotor is spinning.
  • the centrifuge of claim 4 having means for flowing a sample stream through said sample zone substantially 360 around said rotor core.
  • the centrifuge of claim 5 four passages; equally radially spaced for delivery gradient liquid to said gradient zone and four sample recovery passages equally radially spaced.
  • a plurality of first fins extend from said rotor core into the gradient zone adjacent said housing, each of said first fins having an opening to permit the sample zone to pass therethrough and a solid second fin extending from said rotor core into the gradient zone adjacent the housing a sample stream inlet and a sample stream outlet being positioned adjacent opposing vertical surfaces of said second fin and plate means adhered to the bottom and top surfaces of the second fin extending from the gradient zone to the rotor core to prevent by-pass of said sample zone around said second fin.
  • a plurality of first fins extend from said rotor core into the gradient zone adjacent said housing, each of said first fins having an opening to permit the sample zone to pass therethrough and a solid second fin extending from said rotor core into the gradient zone adjacent the housing, a sample stream inlet and a sample stream outlet being positioned adjacent opposing vertical surfaces of said second fin and plate means adhered to the bottom and top surfaces of the second fin extending from the gradient zone to the rotor core to prevent by-pass of said sample zone around said second fin.
  • a method for forming a sample gradient which comprises forming a gradient zone in a spinning centrifuge, flowing a sample through the top surface of a rotor core into a sample zone adjacent to the gradient zone so that the sample passes substantially 360 and a sample gradient is formed in said gradient zone, removing spent sample through the bottom surface of said rotor core, and removing the sample gradient by introducing liquid gradient through the top surface of the rotor core to the gradient zone to displace said sample gradient radially inward and passing said sample gradient out of said core while the rotor is spinning through a passage at the axis of the core.

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US00132663A 1971-04-09 1971-04-09 Continuous flow zonal rotor Expired - Lifetime US3747843A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US13266371A 1971-04-09 1971-04-09

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US3747843A true US3747843A (en) 1973-07-24

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US (1) US3747843A (de)
JP (1) JPS5620069B1 (de)
DE (1) DE7133593U (de)
FR (1) FR2123308B1 (de)
GB (1) GB1341075A (de)

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3858795A (en) * 1973-02-08 1975-01-07 Int Equipment Co Method for washing blood cells
US3862029A (en) * 1973-10-01 1975-01-21 John E Joyce Density gradient fractionator
US4036426A (en) * 1974-02-15 1977-07-19 Pennwalt Corporation Method of cleaning a centrifuge
US5147280A (en) * 1989-04-07 1992-09-15 Alfa-Lavel Separation Ab Energy transformation device
US6736768B2 (en) * 2000-11-02 2004-05-18 Gambro Inc Fluid separation devices, systems and/or methods using a fluid pressure driven and/or balanced approach
US20080035637A1 (en) * 2006-08-09 2008-02-14 Producers Dairy Foods, Inc. Self-supporting liquid container for boxless storage, shipping and display
US20080087613A1 (en) * 2005-06-22 2008-04-17 Gambro Bct, Inc. Apparatus and Method for Separating Discrete Volumes of A Composite Liquid
US20080096750A1 (en) * 2006-10-20 2008-04-24 Navigant Biotechnologies, Llc Methods for Washing a Red Blood Cell Component and for Removing Prions Therefrom
US20110003675A1 (en) * 2009-07-06 2011-01-06 Caridianbct, Inc. Apparatus and Method for Automatically Loading Washing Solution In A Multi-Unit Blood Processor
US8840535B2 (en) 2010-05-27 2014-09-23 Terumo Bct, Inc. Multi-unit blood processor with temperature sensing
US9028388B2 (en) 2010-06-07 2015-05-12 Terumo Bct, Inc. Multi-unit blood processor with volume prediction
US9733805B2 (en) 2012-06-26 2017-08-15 Terumo Bct, Inc. Generating procedures for entering data prior to separating a liquid into components

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4648863A (en) * 1984-02-07 1987-03-10 Edmund Buhler Apparatus for the pure preparation of particles, biological cell systems and colloids

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1795958A (en) * 1930-02-08 1931-03-10 Jr George W Mcfarlane Separator
US3073517A (en) * 1959-04-07 1963-01-15 Beckman Instruments Inc Continuous flow centrifuge apparatus and rotor therefor
US3168474A (en) * 1963-04-25 1965-02-02 Beckman Instruments Inc Centrifuge apparatus
US3291387A (en) * 1964-01-16 1966-12-13 Technicon Instr Continuous centrifugal separator
US3430849A (en) * 1967-08-01 1969-03-04 Atomic Energy Commission Liquid centrifuge for large-scale virus separation
US3498532A (en) * 1967-06-19 1970-03-03 Mse Holdings Ltd Zonal centrifuge attachment
US3519201A (en) * 1968-05-07 1970-07-07 Us Health Education & Welfare Seal means for blood separator and the like
US3536253A (en) * 1969-02-24 1970-10-27 Atomic Energy Commission Zonal centrifuge

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1795958A (en) * 1930-02-08 1931-03-10 Jr George W Mcfarlane Separator
US3073517A (en) * 1959-04-07 1963-01-15 Beckman Instruments Inc Continuous flow centrifuge apparatus and rotor therefor
US3168474A (en) * 1963-04-25 1965-02-02 Beckman Instruments Inc Centrifuge apparatus
US3291387A (en) * 1964-01-16 1966-12-13 Technicon Instr Continuous centrifugal separator
US3498532A (en) * 1967-06-19 1970-03-03 Mse Holdings Ltd Zonal centrifuge attachment
US3430849A (en) * 1967-08-01 1969-03-04 Atomic Energy Commission Liquid centrifuge for large-scale virus separation
US3519201A (en) * 1968-05-07 1970-07-07 Us Health Education & Welfare Seal means for blood separator and the like
US3536253A (en) * 1969-02-24 1970-10-27 Atomic Energy Commission Zonal centrifuge

Cited By (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3858795A (en) * 1973-02-08 1975-01-07 Int Equipment Co Method for washing blood cells
US3862029A (en) * 1973-10-01 1975-01-21 John E Joyce Density gradient fractionator
US4036426A (en) * 1974-02-15 1977-07-19 Pennwalt Corporation Method of cleaning a centrifuge
US5147280A (en) * 1989-04-07 1992-09-15 Alfa-Lavel Separation Ab Energy transformation device
US6736768B2 (en) * 2000-11-02 2004-05-18 Gambro Inc Fluid separation devices, systems and/or methods using a fluid pressure driven and/or balanced approach
US6773389B2 (en) * 2000-11-02 2004-08-10 Gambro Inc Fluid separation devices, systems and/or methods using a fluid pressure driven and/or balanced configuration
US20040164032A1 (en) * 2000-11-02 2004-08-26 Gambro, Inc. Fluid Separation Methods Using a Fluid Pressure Driven and/or Balanced Approach
US7094196B2 (en) 2000-11-02 2006-08-22 Gambro Inc. Fluid separation methods using a fluid pressure driven and/or balanced approach
US7438679B2 (en) 2005-06-22 2008-10-21 Caridianbct Biotechnologies, Llc Apparatus and method for separating volumes of a composite liquid with a balancing assembly
US8070665B2 (en) 2005-06-22 2011-12-06 CaridianBCT, Inc Method for separating discrete volumes of a composite liquid
US20080096749A1 (en) * 2005-06-22 2008-04-24 Navigant Biotechnologies, Llc Apparatus and Method for Separating Discrete Volumes of A Composite Liquid
US20090317305A1 (en) * 2005-06-22 2009-12-24 Caridianbct, Inc. Bag Set for Separating Discrete Volumes of A Composite Liquid
US7674221B2 (en) 2005-06-22 2010-03-09 Caridianbct, Inc. Apparatus for separating discrete volumes of a composite liquid with balancing elements
US7766809B2 (en) 2005-06-22 2010-08-03 Caridianbct, Inc. Apparatus for separating discrete volumes of a composite liquid
US20100273627A1 (en) * 2005-06-22 2010-10-28 Caridianbct, Inc. Method for Separating Discrete Volumes of A Composite Liquid
US20080087613A1 (en) * 2005-06-22 2008-04-17 Gambro Bct, Inc. Apparatus and Method for Separating Discrete Volumes of A Composite Liquid
US20080035637A1 (en) * 2006-08-09 2008-02-14 Producers Dairy Foods, Inc. Self-supporting liquid container for boxless storage, shipping and display
US20080096750A1 (en) * 2006-10-20 2008-04-24 Navigant Biotechnologies, Llc Methods for Washing a Red Blood Cell Component and for Removing Prions Therefrom
US8016736B2 (en) 2006-10-20 2011-09-13 Caridianbct Biotechnologies, Llc Methods for washing a red blood cell component and for removing prions therefrom
US20110003675A1 (en) * 2009-07-06 2011-01-06 Caridianbct, Inc. Apparatus and Method for Automatically Loading Washing Solution In A Multi-Unit Blood Processor
US8840535B2 (en) 2010-05-27 2014-09-23 Terumo Bct, Inc. Multi-unit blood processor with temperature sensing
US9687598B2 (en) 2010-05-27 2017-06-27 Terumo Bct, Inc. Multi-unit blood processor with temperature sensing
US10226567B2 (en) 2010-05-27 2019-03-12 Terumo Bct, Inc. Multi-unit blood processor with temperature sensing
US9028388B2 (en) 2010-06-07 2015-05-12 Terumo Bct, Inc. Multi-unit blood processor with volume prediction
US9849222B2 (en) 2010-06-07 2017-12-26 Terumo Bct, Inc. Multi-unit blood processor with volume prediction
US9733805B2 (en) 2012-06-26 2017-08-15 Terumo Bct, Inc. Generating procedures for entering data prior to separating a liquid into components

Also Published As

Publication number Publication date
JPS5620069B1 (de) 1981-05-11
GB1341075A (en) 1973-12-19
FR2123308A1 (de) 1972-09-08
DE2144070A1 (de) 1972-10-26
DE2144070B2 (de) 1976-08-05
FR2123308B1 (de) 1977-12-23
DE7133593U (de) 1972-03-23

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Owner name: BANQUE PARIBAS, THE EQUITABLE TOWER 787 SEVENTH AV

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNOR:INTERNATIONAL EQUIPMENT COMPANY;REEL/FRAME:005305/0535

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Owner name: INTERNATIONAL EQUIPMENT COMPANY, A CORP OF DE, MAS

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Effective date: 19901005

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Free format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNORS:MATSUOKA, ATSUKO;IWATA, TOSHIO;REEL/FRAME:005304/0046;SIGNING DATES FROM 19900313 TO 19900319