US3681029A - Sample holder and transferring device for a centrifuge - Google Patents

Sample holder and transferring device for a centrifuge Download PDF

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Publication number
US3681029A
US3681029A US27506A US3681029DA US3681029A US 3681029 A US3681029 A US 3681029A US 27506 A US27506 A US 27506A US 3681029D A US3681029D A US 3681029DA US 3681029 A US3681029 A US 3681029A
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US
United States
Prior art keywords
sample
cuvette
reagent
chamber
disk
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
US27506A
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English (en)
Inventor
Stephen I Shapiro
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Adaltis US Inc
Original Assignee
Union Carbide Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Union Carbide Corp filed Critical Union Carbide Corp
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Publication of US3681029A publication Critical patent/US3681029A/en
Assigned to BAKER INSTRUMENTS CORPORATION, A CORP. OF DE reassignment BAKER INSTRUMENTS CORPORATION, A CORP. OF DE ASSIGNMENT OF ASSIGNORS INTEREST. Assignors: UNION CARBIDE CORPORATION
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/07—Centrifugal type cuvettes

Definitions

  • Known rotary photometric analyzers have a series of cuvettes arranged vertically and concentrically around a horizontally rotatable disk so that when the disk is rotated centrifugal force mixes and transfers reagents and samples to the cuvettes. As each cuvette passes a light source the samples are analyzed photometrically by pro jecting a light beam through the bottom'of the cuvette and measuring the absorbence as the beam emerges from the top of the cuvette.
  • the analyzer is provided with a sample holder and transferring device having reagent, sample and transfer chambers disposed in such a geometric configuration that the entire sample is picked up in the reagent and swept into the cuvette.
  • This invention relates to a novel sample holder and transferring device.
  • this invention relates to an apparatus for transferring reagents and samples.
  • this invention relates to a device for dispensing measured volumes of liquids for microanalytical photometric systems.
  • Reagents are placed in the inner-most cavity and serum samples in the center cavity of the sample loading disk which is then indexed and positioned in the rotor with each reagent and serum sample having its respective cuvette.
  • centrifugal force moves the reagents and sample to the outer-most cavity where they are transferred through a small channel to the cuvette.
  • the reagent and sample mix.
  • the filled cuvettes rapidly spin past the fixed light beam and the transmission of light is measured.
  • the geometery of the cavities in the rotary must be such that the reagents and sample are completely transferred into the cuvette. If transfer is not complete, the cuvette may contain an improper concentration of sample and hence give an erroneous reading. In view of the fact that the instrument is particularly useful for bio chemical clinical testing, it is imperative that accuracy be at its optimum.
  • the invention relates to an improved sample holder and transferring device adaptable for use in rotary photometric analyzers.
  • the device is comprised of, in combination, an annular disk having on its upper surface a pluralityof chambers each of which extends outwardly toward the peripheral edge of the disk.
  • the chamber is comprised of at least one reagent chamber disposed at an angle which is less than a right angle to the direction of the force created by the centrifugal field.
  • a sample chamber communicating with the adjacent to the reaction chamber the depth of which is less than the reagent chamber, and a transfer chamber communicating with and adjacent to the sample chamber and disposed at an angle greater than a right angle to the direction of the force created by the centrifugal field.
  • the transfer chamber communicates to the outer peripheral edge of the disk through an exit port. When the rotor is in place in the analyzer, the exit ports communicate with the cuvettes.
  • sample chamber 16, transfer chamber 18 and exit port 20 As is evident from the drawing, when the disk containing reagent and sample commences to rotate, the increasing centrifugal force drives the reagent from its chamber up to the shelf formed by the reagent and sample chambers and sweeps the sample from its chamber over into the transfer chamber. Mixing of reagent and sample begins immediately as the reagent sweeps the sample into the transfer chamber and eventually into the cuvette.
  • the reagent and sample chambers are inclined towards the outer peripheral edge of the disk and the sample chamber is not as deep as the reagent chamber, i.e., it forms a shelf with the reagent chamber, the reagent and sample are kept separated until the rotor disk revolves.
  • the liquid reagent then rises up its outer wall and passes over the bottom of the sample chamber and sweeps the sample up the sample chamber wall and into the transfer chamber.
  • the sample chamber wall should rise to a point just below the upper surface of the rotor disk.
  • the chambers can be of a variety of configurations. For example, they can be cylindrical, oval, square, or the like, as long as the basic geometric configuration is maintained.
  • the rotor disk itself can be constructed out of a variety of materials such as high density linear polyethylene, tetrafluoroethylene, and the 3 like.
  • the surface of the chambers must, of course, be of such material that neither reagents of samples will adhere strongly to it when the rotor is revolving.
  • the device of this invention is employed in conjunction with the instrument described in the foregoing Analytical Biochemistry Journal.
  • a sample holder and transferring device for use in mixing and transferring liquids in a centrifugal field, said device comprised of, in combination an annular disk having on its upper surface a plurality of chambers each of which extends outwardly toward the peripheral edge of the disk, each of said chambers being comprised of at least one reagent chamber disposed at an angle which is less than a right angle to the direction of the force created by the centrifugal field, a sample chamber disposed at an angle which is less than a right angle to the direction of the force created by the centrifugal field, communicating with and adjacent to said reaction chamber, the depth of which is less than said reagent chamber, and a transfer chamber communicating with and adjacent to said sample chamber and disposed at an angle which is greater than a right angle to the direction of the force created by the centrifugal field, said reagent chamber being closest to the center of the disc, the transfer cham- 4 her being closest to the peripheral edge of the disc, and the sample chamber being locatedbetween the reagent chamber and the transfer chamber said

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Optical Measuring Cells (AREA)
US27506A 1970-04-13 1970-04-13 Sample holder and transferring device for a centrifuge Expired - Lifetime US3681029A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US2750670A 1970-04-13 1970-04-13

Publications (1)

Publication Number Publication Date
US3681029A true US3681029A (en) 1972-08-01

Family

ID=21838111

Family Applications (1)

Application Number Title Priority Date Filing Date
US27506A Expired - Lifetime US3681029A (en) 1970-04-13 1970-04-13 Sample holder and transferring device for a centrifuge

Country Status (12)

Country Link
US (1) US3681029A (enrdf_load_stackoverflow)
JP (1) JPS5611886B1 (enrdf_load_stackoverflow)
BE (1) BE765595A (enrdf_load_stackoverflow)
BR (1) BR7102117D0 (enrdf_load_stackoverflow)
CA (1) CA940100A (enrdf_load_stackoverflow)
CH (1) CH531172A (enrdf_load_stackoverflow)
DE (1) DE2117423B2 (enrdf_load_stackoverflow)
FR (1) FR2089490A5 (enrdf_load_stackoverflow)
GB (1) GB1340358A (enrdf_load_stackoverflow)
IL (1) IL36602A (enrdf_load_stackoverflow)
NL (1) NL7104786A (enrdf_load_stackoverflow)
SE (1) SE376480B (enrdf_load_stackoverflow)

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3817632A (en) * 1972-09-21 1974-06-18 Union Carbide Corp Digital subtraction circuit for a centrifugal chemical analyzer of the rotating spectrophotometer type
US3856470A (en) * 1973-01-10 1974-12-24 Baxter Laboratories Inc Rotor apparatus
US3951608A (en) * 1975-01-22 1976-04-20 Ernest Trod Mixing cuvette and timing turntable for providing reaction mixtures
US4309384A (en) * 1980-04-04 1982-01-05 Ernest Trod Chemical analysis cuvette
US4314968A (en) * 1979-10-26 1982-02-09 Jean Guigan Simultaneous analysis apparatus
US4458812A (en) * 1982-08-09 1984-07-10 Instrumentation Laboratory, Inc. Reagent storage vessel
US4470954A (en) * 1983-06-13 1984-09-11 Chiknas Steven G Rotor or carrier for centrifugal analyzer and bead washer
US4689203A (en) * 1984-01-11 1987-08-25 Fluilogic Systems Oy Centrifuge
US5011663A (en) * 1987-07-22 1991-04-30 S E A C S.R.L. Multitest-tube for clinical chemistry analysis for several simultaneous tests
WO1996029137A1 (en) * 1995-03-21 1996-09-26 Charles Jewell Apparatus and method for blood analyses
US5772967A (en) * 1988-02-16 1998-06-30 Wannlund; Jon C. Luminescence test and exposure apparatus
EP1264636A1 (de) * 2001-05-30 2002-12-11 Holger Behnk Vorrichtung und Verfahren zum Untersuchen von Körperflüssigkeiten
US20050170515A1 (en) * 2004-02-04 2005-08-04 Hach Company Analytical rotor system with an analytical signal path
US20050170514A1 (en) * 2004-02-04 2005-08-04 Hach Company Analytical rotor system for method of standard additions testing
US20050169804A1 (en) * 2004-02-04 2005-08-04 Hach Company User-configurable analytical rotor system
US20050170513A1 (en) * 2004-02-04 2005-08-04 Hach Company Analytical rotor system for titration testing
US20050169805A1 (en) * 2004-02-04 2005-08-04 Hach Company Analytical rotor system with a sample chamber
US20070087429A1 (en) * 2003-12-08 2007-04-19 Kazuhito Tanimoto Reaction disk and separation cell for automatic analyzer

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4857190A (en) * 1984-03-02 1989-08-15 Miles Laboratories, Inc. Container for fine separation of blood and blood components
CH685312A5 (de) * 1993-05-03 1995-05-31 Diag Human Ag Vorrichtung zur Blutuntersuchung.

Cited By (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3817632A (en) * 1972-09-21 1974-06-18 Union Carbide Corp Digital subtraction circuit for a centrifugal chemical analyzer of the rotating spectrophotometer type
US3856470A (en) * 1973-01-10 1974-12-24 Baxter Laboratories Inc Rotor apparatus
US3951608A (en) * 1975-01-22 1976-04-20 Ernest Trod Mixing cuvette and timing turntable for providing reaction mixtures
US4314968A (en) * 1979-10-26 1982-02-09 Jean Guigan Simultaneous analysis apparatus
US4309384A (en) * 1980-04-04 1982-01-05 Ernest Trod Chemical analysis cuvette
US4458812A (en) * 1982-08-09 1984-07-10 Instrumentation Laboratory, Inc. Reagent storage vessel
US4470954A (en) * 1983-06-13 1984-09-11 Chiknas Steven G Rotor or carrier for centrifugal analyzer and bead washer
US4689203A (en) * 1984-01-11 1987-08-25 Fluilogic Systems Oy Centrifuge
US5011663A (en) * 1987-07-22 1991-04-30 S E A C S.R.L. Multitest-tube for clinical chemistry analysis for several simultaneous tests
US5772967A (en) * 1988-02-16 1998-06-30 Wannlund; Jon C. Luminescence test and exposure apparatus
US5631166A (en) * 1995-03-21 1997-05-20 Jewell; Charles R. Specimen disk for blood analyses
WO1996029137A1 (en) * 1995-03-21 1996-09-26 Charles Jewell Apparatus and method for blood analyses
US5885528A (en) * 1995-03-21 1999-03-23 Immutech, Inc. Apparatus for blood analysis
US6024883A (en) * 1995-03-21 2000-02-15 Immutech, Inc. Method for blood analyses
EP1264636A1 (de) * 2001-05-30 2002-12-11 Holger Behnk Vorrichtung und Verfahren zum Untersuchen von Körperflüssigkeiten
CN100419428C (zh) * 2001-05-30 2008-09-17 霍尔格·本克 一种用于分析体液的装置和方法
US20070087429A1 (en) * 2003-12-08 2007-04-19 Kazuhito Tanimoto Reaction disk and separation cell for automatic analyzer
US7722812B2 (en) * 2003-12-08 2010-05-25 Wako Pure Chemical Industries, Ltd. Reaction disk and separation cell for automatic analyzer
US20050170515A1 (en) * 2004-02-04 2005-08-04 Hach Company Analytical rotor system with an analytical signal path
US20050170514A1 (en) * 2004-02-04 2005-08-04 Hach Company Analytical rotor system for method of standard additions testing
US20050169804A1 (en) * 2004-02-04 2005-08-04 Hach Company User-configurable analytical rotor system
US20050170513A1 (en) * 2004-02-04 2005-08-04 Hach Company Analytical rotor system for titration testing
US20050169805A1 (en) * 2004-02-04 2005-08-04 Hach Company Analytical rotor system with a sample chamber

Also Published As

Publication number Publication date
FR2089490A5 (enrdf_load_stackoverflow) 1972-01-07
CA940100A (en) 1974-01-15
IL36602A (en) 1973-07-30
JPS5611886B1 (enrdf_load_stackoverflow) 1981-03-17
SE376480B (enrdf_load_stackoverflow) 1975-05-26
NL7104786A (enrdf_load_stackoverflow) 1971-10-15
BR7102117D0 (pt) 1973-02-22
DE2117423B2 (de) 1976-01-29
DE2117423A1 (de) 1971-10-28
CH531172A (fr) 1972-11-30
IL36602A0 (en) 1971-06-23
GB1340358A (en) 1973-12-12
BE765595A (fr) 1971-10-11

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AS Assignment

Owner name: BAKER INSTRUMENTS CORPORATION, 2196 AVENUE C, P.O.

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNOR:UNION CARBIDE CORPORATION;REEL/FRAME:003865/0890

Effective date: 19810512