US3648927A - Syringe-supporting device for centrifuges - Google Patents

Syringe-supporting device for centrifuges Download PDF

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Publication number
US3648927A
US3648927A US103995A US3648927DA US3648927A US 3648927 A US3648927 A US 3648927A US 103995 A US103995 A US 103995A US 3648927D A US3648927D A US 3648927DA US 3648927 A US3648927 A US 3648927A
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US
United States
Prior art keywords
syringe
sleeve
centrifuge
counterweight
shells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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US103995A
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English (en)
Inventor
Lucien Abbe
Charles Sachs
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Individual
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Individual
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Publication date
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Publication of US3648927A publication Critical patent/US3648927A/en
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
    • B04B5/0421—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes pivotably mounted
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components

Definitions

  • the device comprises a pivotal cylindrical sleeve and two identical half-shells between which the syringe is clamped.
  • Said half-shells are provided on the one hand with a groove for locking the cylinder of the syringe in position and on the other hand with a plurality of grooves for locking the plunger of the syringe in the position corresponding to the quantity of blood taken.
  • Said half-shells are inserted in the sleeve in oppositely facing relation so that the pivotal motion of the latter should direct the plunger towards the exterior and the orifice of the syringe towards the axis of the centrifuge.
  • the operation is carried out under a protective oil layer.
  • the blood is taken into a syringe and then injected into a centrifuge bowl beneath an oil layer.
  • the plasma is then collected by separation of the intermediate layer which is located between the oil and the red corpuscles of the blood.
  • the blood-taking operation is carried out directly in a special vacuum tube and this tube is centrifuged without any transfer operation.
  • it is then necessary either to unseal the tube or to carry out a fairly complex sampling operation with a double needle through a rubber stopper.
  • the aim of this invention is to provide a device which makes it possible to solve these problems.
  • the invention is accordingly directed to a device for supporting a syringe on a centrifuge and comprising a cylindrical sleeve which is open at both ends, means for connecting said sleeve to the centrifuge in order to permit of pivotal motion of said sleeve about an axis at right angles to the axis of said sleeve, two identical half-shells which are inserted in said sleeve in order to retain between said half-shells the syringe employed for taking the blood to be centrifuged, each 'halfshell being provided with two portions having different diameters and at the junction of said two portions with a first internal groove for locking the cylinder of the syringe and at the extremity of the portion of smaller diameter with a number of internal grooves of which one groove locks the plunger of the syringe in the position corresponding to the quantity of blood taken.
  • the syringe can thus be held in position between the two halfshells in order that the plunger should remain in contact with the sampled blood and prevent any penetration of air.
  • the shells which are inserted in the sleeve are capable of oscillating with respect to the centrifuge so that the syringe swings upwards to the horizontal position under the action of centrifugal force, the orifice of said syringe being directed towards the axis.
  • the red corpuscles accumulate against the plunger whilst the plasma is located in the vicinity of said orifice. Emptying of the syringe can thus be carried out simply by displacement of the plunger which discharges the plasma towards the exterior. No transfer or introduction of auxiliary components is necessary.
  • the device comprises a counterweight of elongated shape and having an axis which is parallel to that of the sleeve, said counterweight being rigidly fixed to the sleeve and adapted to support the means for connecting said sleeve to the centrifuge.
  • FIG. 1 is an axial sectional view of a supporting device in the position of rotation of the centrifuge
  • FIG. 2 shows the means for connectingthe device to the centrifuge
  • FIG. 3 is an axial sectional view of an alternative embodiment of the device which is also in the centrifugation position;
  • FIG. 4 is a sectional view taken along line I--I of FIG. 3.
  • the syringe-supporting device is essentially constituted by a cylindrical sleeve 1 which is open at both ends, two half-shells 2, 4 which surround the syringe 3 to be centrifuged being inserted in said sleeve.
  • Each halfshell comprises two successive half-cylinders having different diameters.
  • the large-diameter cylinder 2a, 4a fits exactly within the sleeve 1 and is provided at one end with a peripheral annular abutment flange 6 which is applied against said sleeve.
  • the second cylinder 2b, 4b has a slightly larger diameter than that of the plunger of the syringe and preferably has an extension at the end remote from its junction with the cylinder 2a in the form of a cone frustum 5 in which are formed a number of internal annular grooves 8 for locking the extremity of said plunger 10.
  • the half-shells 2b and 4b are additionally provided with an annular groove 12 which is intended to engage over the end flange 13 of the syringe cylinder 14.
  • said cylinder 14 passes freely through the end of each half-shell and extends beyond these latter and beyond the flange 6.
  • the sleeve 1 is in turn provided with two diametrically opposite external longitudinal slideways 18 which are each engaged over a stud 20 carried by one of the arms 22 of a forked member which forms the extremity of a radial arm 24 of said centrifuge.
  • the two half-shells 2 and 4 are placed around the syringe 3, the groove 12 being intended to fit onto the flange 13 whilst one of the grooves 8 engages over the extremity of the plunger 10. Irrespective of the quantity of blood taken, the plurality of grooves 8 makes it possible for the plunger 10 to remain in contact with the blood sample. The plunger therefore closes the orifice of the syringe and prevents air from penetrating into this latter.
  • Rotary motion of the centrifuge then causes pivotal motion of the sleeve 1 about the studs 20 from the vertical position to the horizontal position.
  • the syringe is centrifuged in the position shown in FIG. 1 in which the plunger 10 is directed towards the exterior whilst the blood-sampling orifice is directed towards the axis of the centrifuge.
  • the red corpuscles of the blood are therefore projected towards the plunger 10 and adhere to this latter.
  • the plasma which is separated from said corpuscles remains located between these latter and the orifice of the syringe.
  • the device When centrifugation has been completed, the device returns automatically to the vertical position.
  • the red corpuscles remain in contact with the plunger 10 and the plasma which is lighter remains at the top of the syringe.
  • said syringe has been withdrawn from the supporting device, it is then an easy matter to produce action on the plunger and to discharge the plasma alone into a suitable vessel.
  • the sleeve 1 is rigidly fixed to a counterweight 16 of elongated shape whose axis is parallel to that of the syringe 3 and of the sleeve I.
  • Said counterweight is provided on each lateral face with a longitudinal slideway 28 which is open at the free end of the counterweight but closed opposite to the sleeve 1.
  • Said slideways provide a means for connecting the supporting assembly to the centrifuge.
  • each slideway 28 cooperates with a stud 20 carried by one of the arms 22 of a forked member which forms the extremity of a radial arm 24 of said centrifuge.
  • the syringe is mounted on the centrifuge in a vertical position, the slideways 28 slide over the studs 20 but the rotary motion of the centrifuge causes the counterweight 16 to swing upwards and places said syringe in the horizontal position, the sampling orifice being continuously directed towards the axis of the centrifuge.
  • the assembly automatically returns to the vertical position as soon as the rotary motion stops; the red corpuscles remain in contact with the plunger and the plasma remains at the top of the syringe.
  • said device is not limited to centrifugation of blood but could also serve for other solutions which are intended to be strictly isolated from the surrounding air and are collected in a syringe.
  • the half-shells can be solid and conform substantially to the shape of the syringe or can alternatively be hollow and come into contact with said syringe only at their extremities and around the annular flanges.
  • the small-diameter cylinder or the cone frustum which may form an extension of this latter can be closed.
  • a device for supporting a hypodermic syringe on a centrifuge comprising a cylindrical sleeve which is open at both ends, means for connecting said sleeve to the centrifuge in order to permit of pivotal motion of said sleeve about an axis at right angles to the axis of said sleeve, two identical halfshells which are inserted in said sleeve in order to retain between said half-shells the syringe employed for sampling the blood to be centrifuged, each half-shell being provided with two portions having different diameters and at the junction of said two portions with a first internal groove for locking the cylinder of the syringe and at the extremity of the portion of smaller diameter with a number of internal grooves of which one groove locks the plunger of the syringe in the position corresponding to the quantity of blood taken.
  • each half-shell is provided with a semi-annular peripheral abutment flange which bears on the sleeve.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Ecology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • External Artificial Organs (AREA)
  • Centrifugal Separators (AREA)
US103995A 1970-01-07 1971-01-05 Syringe-supporting device for centrifuges Expired - Lifetime US3648927A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7000354A FR2076223A5 (fr) 1970-01-07 1970-01-07

Publications (1)

Publication Number Publication Date
US3648927A true US3648927A (en) 1972-03-14

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Family Applications (1)

Application Number Title Priority Date Filing Date
US103995A Expired - Lifetime US3648927A (en) 1970-01-07 1971-01-05 Syringe-supporting device for centrifuges

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US (1) US3648927A (fr)
FR (1) FR2076223A5 (fr)
GB (1) GB1296616A (fr)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4586375A (en) * 1984-08-29 1986-05-06 Rca Corporation Reusable centrifuge fixture and methods of making and using same
US5811061A (en) * 1992-02-10 1998-09-22 Baxter International Inc. Method and device for testing blood units for viral contamination
US6132353A (en) * 1996-10-21 2000-10-17 Winkelman; James W. Apparatus and method for separating plasma or serum from the red cells of a blood sample
US6569118B2 (en) 2000-05-25 2003-05-27 Johnnie M. Johnson Adapter and method of attachment for “LUER LOK” receptacles
US20110130263A1 (en) * 2009-09-24 2011-06-02 Del Vecchio Daniel A Syringe centrifuge systems
WO2012093270A1 (fr) * 2011-01-06 2012-07-12 Semmelweis Egyetem Dispositif pour la centrifugation et le traitement complémentaire d'aspirats tissulaires en système clos
US20160030952A1 (en) * 2013-03-14 2016-02-04 Centricycle, Inc. Centrifuge device

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2627857A (en) * 1949-07-21 1953-02-10 Marcelli Attilio Syringe holder
DE894010C (de) * 1951-03-30 1953-10-22 Paul Funke & Co Zentrifuge fuer Butyrometer
US3339837A (en) * 1964-08-31 1967-09-05 Measuring & Scient Equipment L Load carriers and centrifuges
US3420437A (en) * 1967-02-15 1969-01-07 Sorvall Inc Ivan Cell washing centrifuge

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2018837A (en) * 1933-07-12 1935-10-29 Collatz Ewald Centrifugal device
US3190546A (en) * 1959-03-27 1965-06-22 Raccuglia Giovanni Method and apparatus for separating liquid mixtures
FR1384386A (fr) * 1961-07-07 1965-01-04 Becton Dickinson Co Ensemble collecteur de sang

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2627857A (en) * 1949-07-21 1953-02-10 Marcelli Attilio Syringe holder
DE894010C (de) * 1951-03-30 1953-10-22 Paul Funke & Co Zentrifuge fuer Butyrometer
US3339837A (en) * 1964-08-31 1967-09-05 Measuring & Scient Equipment L Load carriers and centrifuges
US3420437A (en) * 1967-02-15 1969-01-07 Sorvall Inc Ivan Cell washing centrifuge

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4586375A (en) * 1984-08-29 1986-05-06 Rca Corporation Reusable centrifuge fixture and methods of making and using same
US5811061A (en) * 1992-02-10 1998-09-22 Baxter International Inc. Method and device for testing blood units for viral contamination
US6132353A (en) * 1996-10-21 2000-10-17 Winkelman; James W. Apparatus and method for separating plasma or serum from the red cells of a blood sample
US6398705B1 (en) * 1996-10-21 2002-06-04 Manfred Grumberg Apparatus for separating plasma or serum from the red cells with a blood sample
US6569118B2 (en) 2000-05-25 2003-05-27 Johnnie M. Johnson Adapter and method of attachment for “LUER LOK” receptacles
USRE43597E1 (en) * 2000-05-25 2012-08-21 Mary Lucille Pilkington Trust Adapter and method of attachment for “LUER LOK” receptacles
US8986185B2 (en) * 2009-09-24 2015-03-24 Lipovera, Llc Syringe centrifuge systems
US20110130263A1 (en) * 2009-09-24 2011-06-02 Del Vecchio Daniel A Syringe centrifuge systems
WO2012093270A1 (fr) * 2011-01-06 2012-07-12 Semmelweis Egyetem Dispositif pour la centrifugation et le traitement complémentaire d'aspirats tissulaires en système clos
US20160030952A1 (en) * 2013-03-14 2016-02-04 Centricycle, Inc. Centrifuge device
US9839921B2 (en) * 2013-03-14 2017-12-12 Sisu Global Health, Inc. Modular centrifuge devices and methods
US10335803B2 (en) * 2013-03-14 2019-07-02 Sisu Global Health, Inc. Modular centrifuge devices and methods
US20190314829A1 (en) * 2013-03-14 2019-10-17 Sisu Global Health, Inc. Modular centrifuge devices and methods
US10792675B2 (en) * 2013-03-14 2020-10-06 Sisu Global Health, Inc. Modular centrifuge devices and methods

Also Published As

Publication number Publication date
GB1296616A (fr) 1972-11-15
FR2076223A5 (fr) 1971-10-15

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