US20230174910A1 - Cell culture device - Google Patents
Cell culture device Download PDFInfo
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- US20230174910A1 US20230174910A1 US18/000,696 US202118000696A US2023174910A1 US 20230174910 A1 US20230174910 A1 US 20230174910A1 US 202118000696 A US202118000696 A US 202118000696A US 2023174910 A1 US2023174910 A1 US 2023174910A1
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- culture
- cell culture
- cover member
- spaces
- culture device
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/06—Plates; Walls; Drawers; Multilayer plates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/18—Open ponds; Greenhouse type or underground installations
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/02—Membranes; Filters
Definitions
- the present invention relates to a cell culture device.
- Cell culture is a method used to culture or grow cells by removing a piece of tissue slice from a multicellular organism and supplying nutrients to the removed piece of tissue in a vessel.
- Animal cells derived from human or animal tissue may be cultured in a state in which the cells are suspended in a medium or attached to a support. Suspended cells may grow independently in a state in which the cells are suspended in a medium, but adherent cells may grow only in a state in which the cells are attached to a culture surface such as a support.
- a culture surface of the cell culture device is surface-modified by injecting a coating solution into the cell culture device so that the adherent cells can be smoothly attached to a culture surface of the cell culture device.
- a washing operation of injecting a separate washing solution into the cell culture device to remove excess coating solution is performed.
- an operation of culturing the adherent cells using a conventional cell culture device has a drawback in that it is very cumbersome because it involves a seeding operation of attaching the cells to the culture surface and an operation of surface-modifying and washing the culture surface.
- the present invention is designed to solve the problems of the related art, and thus it is an object of the present invention to provide a cell culture device capable of facilitating a seeding operation of adherent cells while maintaining mass productivity.
- the present invention provides a cell culture device which includes a body including a plurality of culture spaces formed therethrough in a height direction; and a plate-like cover member fixed to a bottom surface of the body to cover open bottom portions of the culture spaces in order to form a culture surface on which cells are cultured and surface-modified to facilitate smooth attachment of the cells to the culture surface.
- the cover member may include a motif-coated plate-like nanofiber membrane, and a supporting member attached to one surface of the nanofiber membrane through an adhesive layer to support the nanofiber membrane, and an exposed surface of the nanofiber membrane may form the culture surface.
- the nanofiber membrane may include a plurality of cutout portions cut at positions corresponding to the lower rims of the plurality of culture spaces, respectively, so that one surface of the supporting member can be exposed to the outside.
- the cover member may be a plasma-treated plate-like film member, and one surface of the film member may form the culture surface.
- the body may include at least one welding protrusion protruding outward from a bottom surface to a certain height to surround the lower rims of the culture spaces.
- the cover member may further include a release film laminated on one surface thereof.
- the release film may be removed after the surface modification of the cover member is completed.
- the plurality of culture spaces may be formed on the body to form a gap between two neighboring culture spaces.
- the cover member may be made of a non-toxic material.
- the plurality of culture spaces may be formed in the body so that the plurality of culture spaces are arranged in an m ⁇ n matrix structure in width and longitudinal directions of the body, and a display portion may be provided at an upper edge of the body to identify a location of each of the culture spaces.
- a cell culture device can reduce the unit cost of production by facilitating a seeding operation of adherent cells while maintaining mass productivity, and enhance operation convenience in cell culture.
- FIG. 1 is a diagram showing a cell culture device according to one embodiment of the present invention.
- FIG. 2 is a diagram of the cell culture device shown in FIG. 1 , which is viewed in a different direction in a state in which a cover member is separated from a body.
- FIG. 3 is a cross-sectional diagram taken along the line A-A in FIG. 1 .
- FIG. 4 is a cross-sectional diagram taken along the line B-B in FIG. 1 .
- FIG. 5 is a diagram showing a welding protrusion formed on the body in the cell culture device according to one embodiment of the present invention.
- FIG. 6 is a diagram showing one example of the cover member applicable to the cell culture device according to one embodiment of the present invention.
- FIG. 7 is a diagram showing another example of the cover member applicable to the cell culture device according to one embodiment of the present invention.
- a cell culture device 100 includes a body 110 and a cover member 120 or 220 , as shown in FIGS. 1 to 4 .
- the body 110 may provide a culture space 112 for culturing cells. Such a body 110 may provide a plurality of culture spaces 112 to culture a plurality of cells in one culture process at the same time.
- the body 110 may include a plurality of culture spaces 112 formed therethrough in a height direction, and the plurality of culture spaces 112 may be formed in the body 110 so that the plurality of culture spaces 112 are arranged in an m ⁇ n matrix structure (wherein m and n are natural numbers) in width and longitudinal directions of the body 110 .
- the body 110 may include a plurality of compartment walls 114 extending from a bottom surface to a certain height and formed in a hollow shape, as shown in FIGS. 3 and 4 .
- each of the compartment walls 114 formed in a hollow shape may have open top portion and bottom portion. Accordingly, each of the compartment walls 114 may define a culture space 112 with open top portion and bottom portion.
- the body 110 may form a plurality of culture spaces 112 partitioned by the plurality of compartment walls 114 , and a medium for cell culture and cells to be cultured may be separately disposed in the plurality of culture spaces 112 .
- a display portion 118 may be provided at an upper edge of the body 110 to identify a location of each of the culture spaces 112 .
- an operator may easily identify the location of each of the culture spaces 112 even when the plurality of culture spaces 112 are formed on the body 110 .
- each of the compartment walls 114 defining the culture spaces 112 may be integrally connected to lateral portions of different neighboring compartment walls 114 through a connecting portion 115 .
- the body 110 may have the plurality of compartment walls 114 formed through insert injection molding, and the plurality of culture spaces 112 may be defined by the plurality of compartment walls 114 .
- the cell culture device 100 may secure mass productivity and reduce the unit cost of production because the body 110 may be produced through insert injection molding.
- the plurality of culture spaces 112 may be formed in the body 110 to form a gap between two neighboring culture spaces 112 .
- two compartment walls 114 defining two neighboring culture spaces 112 respectively may have upper rims spaced apart at a predetermined distance from each other.
- the connecting portion 115 configured to connect two neighboring compartment walls 114 to each other may be formed to have a lower height than the compartment walls 114 .
- an inwardly recessed groove 116 may be formed at an upper end of the connecting portion 115 , and two neighboring compartment walls 114 may be spaced apart at a predetermined distance in a state in which the upper rims of two neighboring compartment walls 114 are not connected to each other through the groove 116 .
- the medium flowing out through the open top portions of the culture spaces 112 may be blocked from flowing toward the neighboring culture spaces 112 .
- the cover member 120 or 220 may be fixed to the bottom surface of the body 110 to cover the open bottom portions of the culture spaces 112 .
- the cover member 120 or 220 may be provided as a plate-like member having a predetermined area, and may be fixed to the bottom surface of the body 110 to cover all the open bottom portions of the plurality of culture spaces 112 .
- the cover member 120 or 220 may be bonded to the bottom surface of the body 110 through welding using ultrasonic waves, heat, high-frequency waves, and the like.
- the body 110 may include at least one welding protrusion 117 protruding outward from a bottom surface to a certain height to surround the lower rims of the culture spaces 112 , as shown in FIG. 5 .
- the at least one welding protrusion 117 may be melted when the body 110 and the cover member 120 or 220 are fused, so that the open bottom portions of the culture spaces 112 may be sealed while increasing the bonding force between the body 110 and the cover member 120 or 220 .
- a method of fixing the cover member 120 or 220 to the body 110 is not limited to the welding method.
- the cover member 120 or 220 may be attached to the bottom surface of the body 110 through an adhesive layer.
- a method capable of hermetically sealing the open bottom portions of the culture spaces 112 while fixing the cover member 120 or 220 to the body 110 may be carried out using various methods known in the art.
- the cover member 120 or 220 may cover the open bottom portions of the culture spaces 112 as described above, and one surface of the cover member 120 or 220 covering the open bottom portions of the culture spaces 112 may form a culture surface.
- the cover member 120 or 220 may be surface-modified so that the cells may be smoothly attached to a portion of the cover member 120 or 220 forming the culture surface.
- the cells to be cultured may be smoothly attached to one surface (i.e., a culture surface) of the cover member 120 or 220 during a seeding operation.
- the cells included in the medium may be smoothly attached to one surface of the cover member 120 or 220 forming a culture surface. Accordingly, a series of operations such as coating, drying, and washing for smoothly attaching the cells to the culture surface as known in the related art may be omitted.
- a seeding operation for attaching the cells to the culture surface during the cell culture using the cell culture device 100 according to one embodiment of the present invention may be performed very easily, thereby enhancing the operator's convenience.
- the cover member 120 or 220 may be formed of a non-toxic material to smoothly culture the cells attached through the seeding operation.
- the cover member 120 may include a motif-coated plate-like nanofiber membrane 121 , and a supporting member 123 attached to one surface of the nanofiber membrane 121 through an adhesive layer 122 to support the nanofiber membrane 121 , as shown in FIG. 6 .
- the cover member 120 may be fixed to the bottom surface of the body 110 so that the nanofiber membrane 121 covers the open bottom portions of the culture spaces 112 , and one surface of the nanofiber membrane 121 exposed to each of the culture spaces 112 may form a culture surface to which the cells to be cultured are attached.
- the cells to be cultured may be smoothly attached to one surface of the nanofiber membrane 121 , and the cells attached to one surface of the nanofiber membrane 121 may be cultured with nutrients supplied from the medium filled in the culture spaces 112 .
- the nanofiber membrane 121 may include a plurality of cutout portions 124 formed at positions corresponding to the lower rims of the plurality of culture spaces 112 , respectively, so that one surface of the supporting member 123 can be exposed to the outside.
- the cutout portions 124 may be formed at positions corresponding to the lower rims of the compartment walls 114 , and a nanofiber membrane 121 having a smaller area than a cross-sectional area of each of the culture spaces 112 may be disposed inside each of the cutout portions 124 .
- the supporting member 123 may come into direct contact with the bottom surface of the body 110 through the cutout portions 124 , and the entire area of the nanofiber membrane 121 disposed inside each of the cutout portions 124 may be completely accommodated in each of the culture spaces 112 .
- At least one welding protrusion 117 formed on the bottom surface of the body 110 may come into direct contact with the supporting member 123 through the cutout portions 124 .
- the bonding force between the cover member 120 and the body 110 may be enhanced, and the leakage of the medium filled in the culture spaces 112 to the outside of the culture spaces 112 by moving along the nanofiber membrane 121 may be fundamentally blocked.
- the cover member 220 may be a plasma-treated plate-like film member 221 , as shown in FIG. 7 .
- the cells to be cultured may be smoothly attached to a surface of the film member 221 , and the cells attached to the surface of the film member 221 may be cultured with nutrients supplied from the medium filled in the culture spaces 112 .
- the type of the cover member 120 or 220 is not limited thereto, and various materials used for cell culture as known in the art may be used as long as the materials are implemented in a plate-like shape and the cells are easily attached to the materials.
- cover member 120 or 220 may further include a release film 125 laminated on one surface thereof.
- Such a release film 125 may remain attached to one surface of the cover member 120 or 220 during the surface modification of the cover member 120 or 220 , and may be removed from one surface of the cover member 120 or 220 after the surface modification is completed.
- the release film 125 may be attached to one surface of the supporting member 123 or one surface of the film member 221 as described above.
- Such a release film 125 may cover a surface of the cover member 120 or 220 other than the culture surface to which the cells are attached in a process of surface-modifying the nanofiber membrane 121 or the film member 221 .
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Abstract
A cell culture device is provided. The cell culture device according to an exemplary embodiment of the present invention comprises: a body which includes a plurality of culture spaces formed therethrough in the height direction; and a plate-shaped cover member which is fixed to the bottom surface of the body while covering the open bottom portions of the culture spaces to thus form a culture surface where cells are cultured, and is surface-modified so as to allow the cells to be effectively attached to the culture surface.
Description
- The present invention relates to a cell culture device.
- Cell culture is a method used to culture or grow cells by removing a piece of tissue slice from a multicellular organism and supplying nutrients to the removed piece of tissue in a vessel.
- Animal cells derived from human or animal tissue may be cultured in a state in which the cells are suspended in a medium or attached to a support. Suspended cells may grow independently in a state in which the cells are suspended in a medium, but adherent cells may grow only in a state in which the cells are attached to a culture surface such as a support.
- Accordingly, to culture adherent cells using a cell culture device, a culture surface of the cell culture device is surface-modified by injecting a coating solution into the cell culture device so that the adherent cells can be smoothly attached to a culture surface of the cell culture device. After the culture surface of the cell culture device coated with the coating solution is dried for a predetermined time, a washing operation of injecting a separate washing solution into the cell culture device to remove excess coating solution is performed.
- As a result, an operation of culturing the adherent cells using a conventional cell culture device has a drawback in that it is very cumbersome because it involves a seeding operation of attaching the cells to the culture surface and an operation of surface-modifying and washing the culture surface.
- The present invention is designed to solve the problems of the related art, and thus it is an object of the present invention to provide a cell culture device capable of facilitating a seeding operation of adherent cells while maintaining mass productivity.
- To solve the above problems, the present invention provides a cell culture device which includes a body including a plurality of culture spaces formed therethrough in a height direction; and a plate-like cover member fixed to a bottom surface of the body to cover open bottom portions of the culture spaces in order to form a culture surface on which cells are cultured and surface-modified to facilitate smooth attachment of the cells to the culture surface.
- As one example, the cover member may include a motif-coated plate-like nanofiber membrane, and a supporting member attached to one surface of the nanofiber membrane through an adhesive layer to support the nanofiber membrane, and an exposed surface of the nanofiber membrane may form the culture surface.
- In this case, the nanofiber membrane may include a plurality of cutout portions cut at positions corresponding to the lower rims of the plurality of culture spaces, respectively, so that one surface of the supporting member can be exposed to the outside.
- As another example, the cover member may be a plasma-treated plate-like film member, and one surface of the film member may form the culture surface.
- Also, the body may include at least one welding protrusion protruding outward from a bottom surface to a certain height to surround the lower rims of the culture spaces.
- In addition, the cover member may further include a release film laminated on one surface thereof. In this case, the release film may be removed after the surface modification of the cover member is completed.
- Additionally, the plurality of culture spaces may be formed on the body to form a gap between two neighboring culture spaces.
- Also, the cover member may be made of a non-toxic material.
- Further, the plurality of culture spaces may be formed in the body so that the plurality of culture spaces are arranged in an m×n matrix structure in width and longitudinal directions of the body, and a display portion may be provided at an upper edge of the body to identify a location of each of the culture spaces.
- A cell culture device according to the present invention can reduce the unit cost of production by facilitating a seeding operation of adherent cells while maintaining mass productivity, and enhance operation convenience in cell culture.
-
FIG. 1 is a diagram showing a cell culture device according to one embodiment of the present invention. -
FIG. 2 is a diagram of the cell culture device shown inFIG. 1 , which is viewed in a different direction in a state in which a cover member is separated from a body. -
FIG. 3 is a cross-sectional diagram taken along the line A-A inFIG. 1 . -
FIG. 4 is a cross-sectional diagram taken along the line B-B inFIG. 1 . -
FIG. 5 is a diagram showing a welding protrusion formed on the body in the cell culture device according to one embodiment of the present invention. -
FIG. 6 is a diagram showing one example of the cover member applicable to the cell culture device according to one embodiment of the present invention. -
FIG. 7 is a diagram showing another example of the cover member applicable to the cell culture device according to one embodiment of the present invention. - Hereinafter, the embodiments of the present invention will be described in detail with reference to the accompanying drawings to be easily implemented by those having ordinary skill in the art to which the present invention pertains. However, it should be understood that the present invention may be embodied in various different forms, and is not construed as limited to the embodiments set forth herein. In order to clearly describe the present invention, parts not related to the description are omitted, and like parts are denoted by like reference numerals throughout the specification.
- A
cell culture device 100 according to one embodiment of the present invention includes abody 110 and acover member FIGS. 1 to 4 . - The
body 110 may provide aculture space 112 for culturing cells. Such abody 110 may provide a plurality ofculture spaces 112 to culture a plurality of cells in one culture process at the same time. - That is, the
body 110 may include a plurality ofculture spaces 112 formed therethrough in a height direction, and the plurality ofculture spaces 112 may be formed in thebody 110 so that the plurality ofculture spaces 112 are arranged in an m×n matrix structure (wherein m and n are natural numbers) in width and longitudinal directions of thebody 110. - As one example, the
body 110 may include a plurality ofcompartment walls 114 extending from a bottom surface to a certain height and formed in a hollow shape, as shown inFIGS. 3 and 4 . - Herein, each of the
compartment walls 114 formed in a hollow shape may have open top portion and bottom portion. Accordingly, each of thecompartment walls 114 may define aculture space 112 with open top portion and bottom portion. - In this way, the
body 110 may form a plurality ofculture spaces 112 partitioned by the plurality ofcompartment walls 114, and a medium for cell culture and cells to be cultured may be separately disposed in the plurality ofculture spaces 112. - In this case, as shown in
FIG. 1 , adisplay portion 118 may be provided at an upper edge of thebody 110 to identify a location of each of theculture spaces 112. - Accordingly, an operator may easily identify the location of each of the
culture spaces 112 even when the plurality ofculture spaces 112 are formed on thebody 110. - Meanwhile, each of the
compartment walls 114 defining theculture spaces 112 may be integrally connected to lateral portions of different neighboringcompartment walls 114 through a connectingportion 115. - As one example, the
body 110 may have the plurality ofcompartment walls 114 formed through insert injection molding, and the plurality ofculture spaces 112 may be defined by the plurality ofcompartment walls 114. - As a result, the
cell culture device 100 according to one embodiment of the present invention may secure mass productivity and reduce the unit cost of production because thebody 110 may be produced through insert injection molding. - In this case, the plurality of
culture spaces 112 may be formed in thebody 110 to form a gap between two neighboringculture spaces 112. - That is, two
compartment walls 114 defining two neighboringculture spaces 112 respectively may have upper rims spaced apart at a predetermined distance from each other. - For this purpose, the connecting
portion 115 configured to connect two neighboringcompartment walls 114 to each other may be formed to have a lower height than thecompartment walls 114. - Accordingly, an inwardly
recessed groove 116 may be formed at an upper end of the connectingportion 115, and two neighboringcompartment walls 114 may be spaced apart at a predetermined distance in a state in which the upper rims of two neighboringcompartment walls 114 are not connected to each other through thegroove 116. - As a result, even when the medium containing the cells flows out through the open top portions of the
culture spaces 112 while a medium containing the cells is injected toward each of theculture spaces 112 or thebody 110 is transferred to other sites in a state in which each of theculture spaces 112 is filled with the medium, the medium flowing out through the open top portions of theculture spaces 112 may be blocked from flowing toward the neighboringculture spaces 112. - The
cover member body 110 to cover the open bottom portions of theculture spaces 112. - For this purpose, the
cover member body 110 to cover all the open bottom portions of the plurality ofculture spaces 112. - As one example, the
cover member body 110 through welding using ultrasonic waves, heat, high-frequency waves, and the like. - In this case, the
body 110 may include at least onewelding protrusion 117 protruding outward from a bottom surface to a certain height to surround the lower rims of theculture spaces 112, as shown inFIG. 5 . - That is, the at least one
welding protrusion 117 may be melted when thebody 110 and thecover member culture spaces 112 may be sealed while increasing the bonding force between thebody 110 and thecover member - However, a method of fixing the
cover member body 110 is not limited to the welding method. In this case, thecover member body 110 through an adhesive layer. Also, a method capable of hermetically sealing the open bottom portions of theculture spaces 112 while fixing thecover member body 110 may be carried out using various methods known in the art. - Meanwhile, the
cover member culture spaces 112 as described above, and one surface of thecover member culture spaces 112 may form a culture surface. Thecover member cover member - That is, in the
cell culture device 100 according to one embodiment of the present invention, when thecover member culture spaces 112 to form a culture surface is fixed to the bottom surface of thebody 110 in a state in which thecover member cover member - In this way, when a medium containing cells to be cultured is injected into the
culture spaces 112 during cell culture using thecell culture device 100 according to one embodiment of the present invention, the cells included in the medium may be smoothly attached to one surface of thecover member - As a result, a seeding operation for attaching the cells to the culture surface during the cell culture using the
cell culture device 100 according to one embodiment of the present invention may be performed very easily, thereby enhancing the operator's convenience. - In addition, when a preparative operation for seeding the cells to be cultured is fully completed while attaching a plate-
like cover member body 110 in a state in which the surface modification of the plate-like cover member - Herein, the
cover member - As one example, the
cover member 120 may include a motif-coated plate-like nanofiber membrane 121, and a supportingmember 123 attached to one surface of thenanofiber membrane 121 through anadhesive layer 122 to support thenanofiber membrane 121, as shown inFIG. 6 . - In this case, the
cover member 120 may be fixed to the bottom surface of thebody 110 so that thenanofiber membrane 121 covers the open bottom portions of theculture spaces 112, and one surface of thenanofiber membrane 121 exposed to each of theculture spaces 112 may form a culture surface to which the cells to be cultured are attached. - Accordingly, the cells to be cultured may be smoothly attached to one surface of the
nanofiber membrane 121, and the cells attached to one surface of thenanofiber membrane 121 may be cultured with nutrients supplied from the medium filled in theculture spaces 112. - In this case, the
nanofiber membrane 121 may include a plurality ofcutout portions 124 formed at positions corresponding to the lower rims of the plurality ofculture spaces 112, respectively, so that one surface of the supportingmember 123 can be exposed to the outside. - As one example, the
cutout portions 124 may be formed at positions corresponding to the lower rims of thecompartment walls 114, and ananofiber membrane 121 having a smaller area than a cross-sectional area of each of theculture spaces 112 may be disposed inside each of thecutout portions 124. - In this way, in a case in which the
cover member 120 is attached to the bottom surface of thebody 110, the supportingmember 123 may come into direct contact with the bottom surface of thebody 110 through thecutout portions 124, and the entire area of thenanofiber membrane 121 disposed inside each of thecutout portions 124 may be completely accommodated in each of theculture spaces 112. - That is, at least one
welding protrusion 117 formed on the bottom surface of thebody 110 may come into direct contact with the supportingmember 123 through thecutout portions 124. - As a result, the bonding force between the
cover member 120 and thebody 110 may be enhanced, and the leakage of the medium filled in theculture spaces 112 to the outside of theculture spaces 112 by moving along thenanofiber membrane 121 may be fundamentally blocked. - As another example, the
cover member 220 may be a plasma-treated plate-like film member 221, as shown inFIG. 7 . - Accordingly, the cells to be cultured may be smoothly attached to a surface of the
film member 221, and the cells attached to the surface of thefilm member 221 may be cultured with nutrients supplied from the medium filled in theculture spaces 112. - However, the type of the
cover member - Meanwhile, the
cover member release film 125 laminated on one surface thereof. - Such a
release film 125 may remain attached to one surface of thecover member cover member cover member - That is, the
release film 125 may be attached to one surface of the supportingmember 123 or one surface of thefilm member 221 as described above. - Such a
release film 125 may cover a surface of thecover member nanofiber membrane 121 or thefilm member 221. - As a result, it is possible to prevent surface modification of an unnecessary region in the
cover member member 123 in advance. - While one embodiment of the present invention has been described above, the spirit of the present invention is not limited to the embodiments proposed in this specification. Other embodiments may be easily suggested by adding, changing and removing components by those skilled in the art and will fall within the spirit and scope of the present invention.
Claims (10)
1. A cell culture device comprising:
a body including a plurality of culture spaces formed therethrough in a height direction; and
a plate-like cover member having one surface fixed to a bottom surface of the body to cover open bottom portions of the culture spaces in order to form a culture surface on which cells are cultured and surface-modified to facilitate smooth attachment of the cells to the culture surface.
2. The cell culture device of claim 1 , wherein the cover member includes a motif-coated plate-like nanofiber membrane, and a supporting member attached to one surface of the nanofiber membrane through an adhesive layer to support the nanofiber membrane,
and an exposed surface of the nanofiber membrane forms the culture surface.
3. The cell culture device of claim 2 , wherein the nanofiber membrane includes a plurality of cutout portions cut at positions corresponding to the lower rims of the plurality of culture spaces, respectively, so that one surface of the supporting member can be exposed to the outside.
4. The cell culture device of claim 1 , wherein the cover member is a plasma-treated plate-like film member, and one surface of the film member forms the culture surface.
5. The cell culture device of claim 1 , wherein the body includes at least one welding protrusion protruding outward from a bottom surface to a certain height to surround the lower rims of the culture spaces.
6. The cell culture device of claim 1 , wherein the cover member further includes a release film laminated on one surface thereof.
7. The cell culture device of claim 6 , wherein the release film is removed from the cover member after the surface modification of the cover member is completed.
8. The cell culture device of claim 1 , wherein the plurality of culture spaces are formed in the body to form a gap between two neighboring culture spaces.
9. The cell culture device of claim 1 , wherein the cover member is made of a non-toxic material.
10. The cell culture device of claim 1 , wherein the plurality of culture spaces are formed in the body so that the plurality of culture spaces are arranged in an m×n matrix structure in width and longitudinal directions of the body, and
a display portion is provided at an upper edge of the body to identify a location of each of the culture spaces.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
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PCT/KR2021/006668 WO2021246726A1 (en) | 2020-06-05 | 2021-05-28 | Cell culture device |
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US5232838A (en) * | 1991-12-09 | 1993-08-03 | Minnesota Mining And Manufacturing Company | Culture media device and method of use |
US20070274871A1 (en) * | 2006-05-23 | 2007-11-29 | Genetix Limited | Well plate |
US10563167B2 (en) * | 2010-01-29 | 2020-02-18 | Dai Nippon Printing Co., Ltd. | Microorganism culture sheet |
US9074172B2 (en) * | 2012-08-21 | 2015-07-07 | Jed K. Johnson | Culture plate with fiber-coated bottom surface |
JP6384501B2 (en) * | 2013-09-11 | 2018-09-05 | 大日本印刷株式会社 | Cell culture vessel |
CN109196152B (en) * | 2016-06-13 | 2021-10-22 | 阿莫绿色技术有限公司 | Yarn for cell culture scaffold and fabric comprising same |
KR102504813B1 (en) * | 2016-06-20 | 2023-02-28 | 주식회사 아모라이프사이언스 | Cell culture apparatus |
JPWO2018042532A1 (en) * | 2016-08-31 | 2019-03-22 | 株式会社メニコン | Cell culture vessel |
KR102332822B1 (en) * | 2018-11-14 | 2021-11-30 | 주식회사 아모그린텍 | Cell culture device |
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