US20230041584A1

US20230041584A1 - Novel prodrugs and conjugates of dimethyltryptamine

Info

Publication number: US20230041584A1
Application number: US17/836,984
Authority: US
Inventors: Robert B. Perni; Glenn Short; Srinivas G. Rao
Original assignee: Atai Life Sciences AG; JMD Pharma Creativity LLC
Current assignee: Atai Life Sciences US Inc; Atai Therapeutics Inc; Viridia Life Sciences Inc
Priority date: 2021-06-09
Filing date: 2022-06-09
Publication date: 2023-02-09
Also published as: US20230357146A1; MX2023014620A; US12065405B2; IL309210A; AU2022287974A1; EP4352043A1; US11643391B2; US20230066720A1; CL2023003652A1; WO2022261383A1; JP2024522174A; KR20240019152A; CA3221823A1

Abstract

Provided herein are compounds of Formula (I), or pharmaceutically acceptable salt thereof, wherein R1, R2, R3, R4, R5, R8, R9, X, Y, Z, W, and m are defined herein. Also provided herein are pharmaceutical compositions comprising a compound of Formula (I) or pharmaceutically acceptable salt thereof and a pharmaceutically acceptable excipient.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS

The present application claims priority to U.S. Provisional Patent Application No. 63/208,874 filed Jun. 9, 2021, and U.S. Provisional Application No. 63/229,879 filed Aug. 5, 2021, and which are hereby incorporated by reference in their entirety.

BACKGROUND OF THE INVENTION

N,N-dimethyltryptamine (DMT) is a naturally occurring compound found in numerous plant species and botanical preparations, such as the hallucinogenic infusion known as ayahuasca, and classified as a classic serotonergic psychedelic that induces intense modifications in perception, emotion, and cognition in humans. At higher doses, DMT has a rapid onset, intense psychedelic effects, and a relatively short duration of action with an estimated half-life of less than fifteen minutes. Like other hallucinogens in the tryptamine family, DMT binds to serotonin receptors to produce euphoria and psychedelic effects. Unfortunately, DMT is metabolically unstable and is readily converted by monoamine oxidases (MAO's) to indoleacetic acid and N-oxidation metabolites resulting in poor oral bioavailability.
Serotonergic psychedelics have also demonstrated promising antidepressant, anxiolytic, and anti-addictive properties.
There remains a need for improved prodrugs of tryptamines, such as N,N-dimethyltryptamine (DMT).

SUMMARY OF THE INVENTION

In one aspect, the present disclosure provides a compound of Formula (I):

- or a pharmaceutically acceptable salt thereof;
- wherein,
- R₁and R₂are independently hydrogen, alkyl, cycloalkyl, or R₁and R₂together with the atoms to which they are attached form a heterocycle;
- X, Y and Z are independently hydrogen, halogen, —O—, —S—, —NR_A—, or —(P═O)—OR_A(—OR′), wherein R_Ais hydrogen or alkyl;
- R′, R₃, R₄, R₅and R₆are independently absent, hydrogen, alkyl, —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH)(C═NH)—NH₂, —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or

- R₇and R₈are independently hydrogen or alkyl;
- R₉is hydrogen, alkyl, or halogen, preferably a bromo
- L and M are independently alkyl or aryl;
- W is oxygen, sulfur or NR₆;
- n is an integer from 2-7; and
- m is an integer from 1-4,
- wherein at least one of R′, R₃, R₄, R₅and R₆is —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or

In some embodiments, the compound of Formula (I) is a compound of the Formula (IV):

- wherein R₁₀is —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or

- A is a pharmaceutically acceptable anion and
- wherein R₁, R₂, R₃, R₄, R₅, R₈, R₉, X, Y, Z, W, and m are defined herein.

In some embodiments, the compound of Formula (I) is a compound of the formula:

- wherein R₁, R₁₀, R₃, R₄, R₅, R₈, R₉, X, Y, Z, W, and m are defined herein.

In some embodiments, the present disclosure provides a compound of Formula (III):

- or a pharmaceutically acceptable salt thereof;
- wherein,
- R₁and R₂are independently hydrogen, alkyl, cycloalkyl, or R₁and R₂together with the atoms to which they are attached form a heterocycle;
- X, Y and Z are independently hydrogen, halogen, —O—, —S—, —NR_A—, or —(P═O)—OR_A(—OR′),
- wherein R_Ais hydrogen or alkyl,
- R′, R₃, R₄, and R₅are independently absent, hydrogen, alkyl, —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH)(C═NH)—NH₂, —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or

- R₇and R₈are independently hydrogen or alkyl;
- R₉is hydrogen, alkyl, or halogen;
- L and M are independently alkyl or aryl;
- n is an integer from 2-7; and
- m is an integer from 1-4,
- wherein at least one of R′, R₃, R₄, and R₅is —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or

In some embodiments, the compound of Formula (V) is a compound of the formula:

- wherein R₁₀is —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH)(C═NH)—NH₂, —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or

- A is a pharmaceutically acceptable anion,
- and wherein R₁, R₂, R₃, R₄, R₅, R₈, R₉, X, Y, Z, and m are defined herein.

In some embodiments, the present disclosure provides a pharmaceutical composition comprising a compound of Formula (I) or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable excipient.
In some embodiments, the present disclosure provides a pharmaceutical composition comprising a compound of Formula (II) or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable excipient.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows PK studies of N-phosphonooxymethyl prodrug 6-1-2 (VLS-02-23-0) and metabolite (VLS-02-023-10) following intravenous (1 mg/kg) and oral administration (30 mg/kg) to male CD1 mice (in plasma).

FIG. 2 shows PK studies of N-phosphonooxymethyl prodrug 6-1-2 (VLS-02-23-0) and metabolite (VLS-02-023-10) following intravenous (1 mg/kg) and oral administration (30 mg/kg) to male CD1 mice (in the brain).

DETAILED DESCRIPTION

Throughout this disclosure, various patents, patent applications and publications are referenced. The disclosures of these patents, patent applications and publications in their entireties are incorporated into this disclosure by reference for all purposes in order to more fully describe the state of the art as known to those skilled therein as of the date of this disclosure. This disclosure will govern in the instance that there is any inconsistency between the patents, patent applications and publications cited and this disclosure.

Definitions

For convenience, certain terms employed in the specification, examples and claims are collected here. Unless defined otherwise, all technical and scientific terms used in this disclosure have the same meanings as commonly understood by one of ordinary skill in the art to which this disclosure belongs.
The term “about” when immediately preceding a numerical value means a range (e.g., plus or minus 10% of that value). For example, “about 50” can mean 45 to 55, “about 25,000” can mean 22,500 to 27,500, etc., unless the context of the disclosure indicates otherwise, or is inconsistent with such an interpretation. For example in a list of numerical values such as “about 49, about 50, about 55, . . . ”, “about 50” means a range extending to less than half the interval(s) between the preceding and subsequent values, e.g., more than 49.5 to less than 50.5. Furthermore, the phrases “less than about” a value or “greater than about” a value should be understood in view of the definition of the term “about” provided herein. Similarly, the term “about” when preceding a series of numerical values or a range of values (e.g., “about 10, 20, 30” or “about 10-30”) refers, respectively to all values in the series, or the endpoints of the range.
The terms “administer,” “administering” or “administration” as used herein refer to administering a compound or pharmaceutically acceptable salt of the compound or a composition or formulation comprising the compound or pharmaceutically acceptable salt of the compound to a patient.
The term “pharmaceutically acceptable salts” includes both acid and base addition salts. Pharmaceutically acceptable salts include those obtained by reacting the active compound functioning as a base, with an inorganic or organic acid to form a salt, for example, salts of hydrochloric acid, sulfuric acid, phosphoric acid, methanesulfonic acid, camphorsulfonic acid, oxalic acid, maleic acid, succinic acid, citric acid, formic acid, hydrobromic acid, benzoic acid, tartaric acid, fumaric acid, salicylic acid, mandelic acid, carbonic acid, etc. The acids that may be used to prepare pharmaceutically acceptable acid addition salts of such basic compounds are those that form non-toxic acid addition salts, i.e., salts containing pharmaceutically acceptable anions, including but not limited to malate, oxalate, chloride, bromide, iodide, nitrate, acetate, tartrate, oleate, fumarate, formate, benzoate, glutamate, methanesulfonate, benzenesulfonate, and p-toluenesulfonate salts. Base addition salts include but are not limited to, ethylenediamine, N-methyl-glucamine, lysine, arginine, ornithine, choline, N,N′-dibenzylethylenediamine, chloroprocaine, diethanolamine, procaine, N-benzylphenethylamine, diethylamine, piperazine, tris-(hydroxymethyl)-aminomethane, tetramethylammonium hydroxide, triethylamine, dibenzylamine, ephenamine, dehydroabietylamine, N-ethylpiperidine, benzylamine, tetramethylammonium, tetraethylammonium, methylamine, dimethylamine, trimethylamine, ethylamine, basic amino acids, e.g., lysine and arginine dicyclohexylamine and the like. Examples of metal salts include lithium, sodium, potassium, magnesium, calcium salts and the like. Examples of ammonium and alkylated ammonium salts include ammonium, methylammonium, dimethylammonium, trimethylammonium, ethylammonium, hydroxyethylammonium, diethylammonium, butylammonium, tetramethylammonium salts and the like. Examples of organic bases include lysine, arginine, guanidine, diethanolamine, choline and the like. Those skilled in the art will further recognize that acid addition salts may be prepared by reaction of the compounds with the appropriate inorganic or organic acid via any of a number of known methods.
The term “treating” as used herein with regard to a patient, refers to improving at least one symptom of the patient's disorder. In some embodiments, treating can be improving, or at least partially ameliorating a disorder or one or more symptoms of a disorder.
The term “preventing” as used herein with regard to a patient or subject, refers to preventing the onset of disease development if none had occurred, preventing the disease or disorder from occurring in a subject or a patient that may be predisposed to the disorder or disease but has not yet been diagnosed as having the disorder or disease, and/or preventing further disease/disorder development if already present.
The term “therapeutically effective” applied to dose or amount refers to that quantity of a compound or pharmaceutical formulation that is sufficient to result in a desired clinical benefit after administration to a patient in need thereof.
When a range of values is listed, it is intended to encompass each value and sub-range within the range. For example, “C₁-C₆alkyl” is intended to encompass C₁, C₂, C₃, C₄, C₅, C₆, C_1-6, C_1-5, C_1-4, C_1-3, C_1-2, C_2-6, C_2-5, C_2-4, C_2-3, C_3-6, C_3-5, C_3-4, C_4-6, C_4-5, and C_5-6alkyl.
“Alkyl” or “alkyl group” refers to a fully saturated, straight or branched hydrocarbon chain having from one to twelve carbon atoms, and which is attached to the rest of the molecule by a single bond. Alkyls comprising any number of carbon atoms from 1 to 12 are included. An alkyl comprising up to 12 carbon atoms is a C₁-C₁₂alkyl, an alkyl comprising up to 10 carbon atoms is a C₁-C₁₀alkyl, an alkyl comprising up to 6 carbon atoms is a C₁-C₆alkyl and an alkyl comprising up to 5 carbon atoms is a C₁-C₅alkyl. A C₁-C₅alkyl includes C₅alkyls, C₄alkyls, C₃alkyls, C₂alkyls and C₁alkyl (i.e., methyl). A C₁-C₆alkyl includes all moieties described above for C₁-C₅alkyls but also includes C₆alkyls. A C₁-C₁₀alkyl includes all moieties described above for C₁-C₅alkyls and C₁-C₆alkyls, but also includes C₇, C₈, C₉and C₁₀alkyls. Similarly, a C₁-C₁₂alkyl includes all the foregoing moieties, but also includes C₁₁and C₁₂alkyls. Non-limiting examples of C₁-C₁₂alkyl include methyl, ethyl, n-propyl, i-propyl, sec-propyl, n-butyl, i-butyl, sec-butyl, t-butyl, n-pentyl, t-amyl, n-hexyl, n-heptyl, n-octyl, n-nonyl, n-decyl, n-undecyl, and n-dodecyl. Unless stated otherwise specifically in the specification, an alkyl group can be optionally substituted.
“Aryl” refers to a hydrocarbon ring system comprising hydrogen, 6 to 18 carbon atoms and at least one aromatic ring, and which is attached to the rest of the molecule by a single bond. For purposes of this disclosure, the aryl can be a monocyclic, bicyclic, tricyclic or tetracyclic ring system, which can include fused or bridged ring systems. Aryls include, but are not limited to, aryls derived from aceanthrylene, acenaphthylene, acephenanthrylene, anthracene, azulene, benzene, chrysene, fluoranthene, fluorene, as-indacene, s-indacene, indane, indene, naphthalene, phenalene, phenanthrene, pleiadene, pyrene, and triphenylene. Unless stated otherwise specifically in the specification, the “aryl” can be optionally substituted.
“Cycloalkyl” refers to a stable non-aromatic monocyclic or polycyclic fully saturated hydrocarbon consisting solely of carbon and hydrogen atoms, which can include fused, bridged, or spirocyclic ring systems, having from three to twenty carbon atoms (e.g., having from three to ten carbon atoms) and which is attached to the rest of the molecule by a single bond. Monocyclic cycloalkyls include, for example, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, and cyclooctyl. Polycyclic cycloalkyls include, for example, adamantyl, norbornyl, decalinyl, 7,7-dimethyl-bicyclo[2.2.1]heptanyl, and the like. Unless otherwise stated specifically in the specification, a cycloalkyl group can be optionally substituted.
“Heterocyclyl,” “heterocyclic ring” or “heterocycle” refers to a stable saturated, unsaturated, or aromatic 3- to 20-membered ring which consists of two to nineteen carbon atoms and from one to six heteroatoms selected from the group consisting of nitrogen, oxygen and sulfur, and which is attached to the rest of the molecule by a single bond. Heterocyclyl or heterocyclic rings include heteroaryls, heterocyclylalkyls, heterocyclylalkenyls, and hetercyclylalkynyls. Unless stated otherwise specifically in the specification, the heterocyclyl can be a monocyclic, bicyclic, tricyclic or tetracyclic ring system, which can include fused, bridged, or spirocyclic ring systems; and the nitrogen, carbon or sulfur atoms in the heterocyclyl can be optionally oxidized; the nitrogen atom can be optionally quaternized; and the heterocyclyl can be partially or fully saturated. Examples of such heterocyclyl include, but are not limited to, dioxolanyl, thienyl[1,3]dithianyl, decahydroisoquinolyl, imidazolinyl, imidazolidinyl, isothiazolidinyl, isoxazolidinyl, morpholinyl, octahydroindolyl, octahydroisoindolyl, 2-oxopiperazinyl, 2-oxopiperidinyl, 2-oxopyrrolidinyl, oxazolidinyl, piperidinyl, piperazinyl, 4-piperidonyl, pyrrolidinyl, pyrazolidinyl, quinuclidinyl, thiazolidinyl, tetrahydrofuryl, trithianyl, tetrahydropyranyl, thiomorpholinyl, thiamorpholinyl, 1-oxo-thiomorpholinyl, and 1,1-dioxo-thiomorpholinyl. Unless stated otherwise specifically in the specification, a heterocyclyl group can be optionally substituted.
“Heteroaryl” refers to a 5- to 20-membered ring system comprising hydrogen atoms, one to nineteen carbon atoms, one to six heteroatoms selected from the group consisting of nitrogen, oxygen and sulfur, at least one aromatic ring, and which is attached to the rest of the molecule by a single bond. For purposes of this disclosure, the heteroaryl can be a monocyclic, bicyclic, tricyclic or tetracyclic ring system, which can include fused or bridged ring systems; and the nitrogen, carbon or sulfur atoms in the heteroaryl can be optionally oxidized; the nitrogen atom can be optionally quaternized. Examples include, but are not limited to, azepinyl, acridinyl, benzimidazolyl, benzothiazolyl, benzindolyl, benzodioxolyl, benzofuranyl, benzooxazolyl, benzothiazolyl, benzothiadiazolyl, benzo[b][1,4]dioxepinyl, 1,4-benzodioxanyl, benzonaphthofuranyl, benzoxazolyl, benzodioxolyl, benzodioxinyl, benzopyranyl, benzopyranonyl, benzofuranyl, benzofuranonyl, benzothienyl (benzothiophenyl), benzotriazolyl, benzo[4,6]imidazo[1,2-a]pyridinyl, carbazolyl, cinnolinyl, dibenzofuranyl, dibenzothiophenyl, furanyl, furanonyl, isothiazolyl, imidazolyl, indazolyl, indolyl, indazolyl, isoindolyl, indolinyl, isoindolinyl, isoquinolyl, indolizinyl, isoxazolyl, naphthyridinyl, oxadiazolyl, 2-oxoazepinyl, oxazolyl, oxiranyl, 1-oxidopyridinyl, 1-oxidopyrimidinyl, 1-oxidopyrazinyl, 1-oxidopyridazinyl, 1-phenyl-1H-pyrrolyl, phenazinyl, phenothiazinyl, phenoxazinyl, phthalazinyl, pteridinyl, purinyl, pyrrolyl, pyrazolyl, pyridinyl, pyrazinyl, pyrimidinyl, pyridazinyl, quinazolinyl, quinoxalinyl, quinolinyl, quinuclidinyl, isoquinolinyl, tetrahydroquinolinyl, thiazolyl, thiadiazolyl, triazolyl, tetrazolyl, triazinyl, and thiophenyl (i.e. thienyl). Unless stated otherwise specifically in the specification, a heteroaryl group can be optionally substituted.
The term “substituted” used herein means any of the groups described herein (e.g., alkyl, alkenyl, alkynyl, alkoxy, aryl, cycloalkyl, cycloalkenyl, haloalkyl, heterocyclyl, and/or heteroaryl) wherein at least one hydrogen atom is replaced by a bond to a non-hydrogen atoms such as, but not limited to: a halogen atom such as F, Cl, Br, and I; an oxygen atom in groups such as hydroxyl groups, alkoxy groups, and ester groups; a sulfur atom in groups such as thiol groups, thioalkyl groups, sulfone groups, sulfonyl groups, and sulfoxide groups; a nitrogen atom in groups such as amines, amides, alkylamines, dialkylamines, arylamines, alkylarylamines, diarylamines, N-oxides, imides, and enamines; a silicon atom in groups such as trialkylsilyl groups, dialkylarylsilyl groups, alkyldiarylsilyl groups, and triarylsilyl groups; and other heteroatoms in various other groups. “Substituted” also means any of the above groups in which one or more hydrogen atoms are replaced by a higher-order bond (e.g., a double- or triple-bond) to a heteroatom such as oxygen in oxo, carbonyl, carboxyl, and ester groups; and nitrogen in groups such as imines, oximes, hydrazones, and nitriles. For example, “substituted” includes any of the above groups in which one or more hydrogen atoms are replaced with —NR_gR_h, —NR_gC(═O)R_h, —NR_gC(═O)NR_gR_h, —NR_gC(O)OR_h, —NR_gSO₂R_h, —OC(═O)NR_gR_h, —OR_g, —SR_g, —SOR_g, —SO₂R_g, —OSO₂R_g, —SO₂OR_g, ═NSO₂R_g, and —SO₂NR_gR_h. “Substituted” also means any of the above groups in which one or more hydrogen atoms are replaced with —C(═O)R_g, —C(═O)OR_g, —C(═O)NR_gR_h, —CH₂SO₂R_g, —CH₂SO₂NR_gR_h. In the foregoing, R_gand R_hare the same or different and independently hydrogen, alkyl, alkenyl, alkynyl, alkoxy, alkylamino, thioalkyl, aryl, aralkyl, cycloalkyl, cycloalkenyl, cycloalkylalkyl, haloalkyl, haloalkenyl, haloalkynyl, heterocyclyl, N-heterocyclyl, heterocyclylalkyl, heteroaryl, N-heteroaryl and/or heteroarylalkyl. “Substituted” further means any of the above groups in which one or more hydrogen atoms are replaced by a bond to an amino, cyano, hydroxyl, imino, nitro, oxo, thioxo, halo, alkyl, alkenyl, alkynyl, alkoxy, alkylamino, thioalkyl, aryl, aralkyl, cycloalkyl, cycloalkenyl, cycloalkylalkyl, haloalkyl, haloalkenyl, haloalkynyl, heterocyclyl, N-heterocyclyl, heterocyclylalkyl, heteroaryl, N-heteroaryl and/or heteroarylalkyl group. In addition, each of the foregoing substituents can also be optionally substituted with one or more of the above substituents.

Compounds

The present disclosure provides compounds that are prodrugs of tryptamines and relates scaffolds, for example N,N-dimethyltryptamine (DMT) as well as pharmaceutical compositions thereof.
DMT is metabolically unstable and is readily converted by monoamine oxidases (MAO's) to indoleacetic acid and N-oxidation metabolites resulting in poor oral bioavailability. In some embodiments, the compounds of the present disclosure allow for the controlled release of DMT. Advantages of compounds of the present disclosure may include increased metabolic stability, increased absorption, decreased maximal plasma concentrations of parent drug DMT over time, and less frequent dosing. In some embodiments, compounds of the present disclosure prevent or inhibit N-oxidation to promote oral bioavailability and increased exposure.
In some embodiments, compounds of the present disclosure comprise an enacarbil moiety. Without being bound by theory such compounds may for example, increase bioavailability by virtue of active transport of the prodrug in the small intestine by high-capacity nutrient transporters, including mono-carboxylate transporter-1 (MCT-1). DMT-enacarbil prodrugs may also reduce abuse potential by preventing absorption by snorting or insufflation.
In some embodiments, the present disclosure provides a compound of Formula (I):

- R₇and R₈are independently hydrogen or alkyl;
- R₉is hydrogen, alkyl, or halogen;
- L and M are independently alkyl or aryl;
- W is oxygen, sulfur or NR₆;
- n is an integer from 2-7; and
- m is an integer from 1-4,
- wherein at least one of R′, R₃, R₄, R₅and R₆is —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or

In some embodiments, provided herein is a compound of Formula (II)

- or a pharmaceutically acceptable salt thereof;
- wherein,
- R₁and R₂are independently hydrogen, alkyl, cycloalkyl, or R₁and R₂together with the atoms to which they are attached form a heterocycle;
- X, Y and Z are independently hydrogen, halogen, —O—, —S—, —NR_A—, or —(P═O)—OR_A(—OR′), wherein R_Ais hydrogen or alkyl;
- R′, R₃, R₄, R₅and R₆are independently absent, hydrogen, alkyl, —(C═O)-alkyl, —(C═O)CHR₇, —(C═O)(CH₂)_n(NH₂), —(C)CH(NH₂)(CH₃), —(C═O)CH(NH₂), (CH₂)_n(NH)(C═NH), or —(C═O)(CH₂)_n(NH)(C═NH)—NH₂;
- R₇and R₈are independently hydrogen or alkyl;
- R₉is hydrogen, alkyl, or halogen;
- L and M are independently alkyl or aryl;
- W is oxygen, sulfur or NR₆;
- n is an integer from 2-7; and
- m is an integer from 1-4,
- wherein at least one of R′, R₃, R₄, and R₅and R₆is —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or

In some embodiments, R₉is a halogen. In some embodiments, R₉is bromo.
In some embodiments, provided herein is a compound of Formula (III):

- or a pharmaceutically acceptable salt thereof;
- wherein,
- R₁and R₂are independently hydrogen, alkyl, cycloalkyl, or R₁and R₂together with the atoms to which they are attached form a heterocycle;
- X, Y and Z are independently hydrogen, halogen, —O—, —S—, —NR_A—, —O(P═O)—OR_A(—OR′), or —(P═O)—OR_A(—OR′), wherein R_Ais hydrogen or alkyl,
- R′, R₃, R₄, and R₅are independently absent, hydrogen, alkyl, —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH)(C═NH)—NH₂, —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or

In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), R₁and R₂are independently hydrogen, alkyl, or cycloalkyl, or R₁and R₂together with the atoms to which they are attached form a heterocycle.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), R₁and R₂are independently hydrogen, alkyl, or cycloalkyl.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), R₁and R₂together with the atoms to which they are attached form a heterocycle.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), R₁and R₂are independently hydrogen or C₁-C₆alkyl.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), R₁and R₂are independently C₁-C₆alkyl.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), R₁and R₂are independently methyl, ethyl, propyl, and isopropyl.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), R₁and R₂are methyl.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), X, Y and Z are independently hydrogen, halogen, —O—, —S—, —NR_A—, —O(P═O)—OR_A(—OR′), or —(P═O)—OR_A(—OR′).
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), X, Y and Z are H.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), two of X, Y and Z are hydrogen.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), two of X, Y and Z are hydrogen and the non-hydrogen X, Y or Z is —O—, —S—, —NR_A—, —O(P═O)—OR_A(—OR′), or —(P═O)—OR_A(—OR′), wherein R_Ais hydrogen or alkyl.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), X and Y are hydrogen; and Z is —O—, —S—, —NR_A—, —O(P═O)—OR_A(—OR′), or —(P═O)—OR_A(—OR′), wherein R_Ais hydrogen or alkyl.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), X and Z are hydrogen; and Y is —O—, —S—, —NR_A—, —O(P═O)—OR_A(—OR′), or —(P═O)—OR_A(—OR′), wherein R_Ais hydrogen or alkyl.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), Y and Z are hydrogen; and X is —O—, —S—, —NR_A—, —O(P═O)—OR_A(—OR′), or —(P═O)—OR_A(—OR′), wherein R_Ais hydrogen or alkyl.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), at least one of X, Y, or Z is —O(P═O)—OR_A(—OR′).
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), X is —O(P═O)—OR_A(—OR′) and R₃is absent.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), Y is —O(P═O)—OR_A(—OR′) and R₄is absent.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), Z is —O(P═O)—OR_A(—OR′) and R₅is absent.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), R′ is hydrogen or alkyl.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), R_Ais hydrogen or alkyl.
In some embodiments of the compounds of Formula (I) or Formula (II), R′, R₃, R₄, R₅and R₆are independently absent, hydrogen, alkyl, —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH)(C═NH)—NH₂, —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or
wherein at least one of R′, R₃, R₄, R₅and R₆is —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or
In some embodiments of the compounds of Formula (III), R′, R₃, R₄, and R₅are independently absent, hydrogen, alkyl, —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH)(C═NH)—NH₂, —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or
wherein at least one of R′, R₃, R₄, and R₅is —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), R′ is absent, hydrogen, alkyl, —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH)(C═NH)—NH₂, —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), R′ is hydrogen or alkyl.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), R₃is:
—(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), R₄is:
—(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), R₅is:
—(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or
In some embodiments of the compounds of Formula (I), R₆is: —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or
In some embodiments of the compounds of Formula (I), X, Y and Z are H; W is NR₆; and R₆is —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or
In some embodiments of the compounds of Formula (I), at least one of R′, R₃, R₄, R₅and R₆is —(C═O)(CH₂)_n(NH₂) and n is 3.
In some embodiments of the compounds of Formula (I), at least one of R′, R₃, R₄, R₅and R₆is —(C═O)CH(NH₂)(CH₂)_n(NH₂), and n is 4.
In some embodiments of the compounds of Formula (I), at least one of R′, R₃, R₄, R₅and R₆is —(C═O)(CH₂)_n(NH)(C═NH)—NH₂and n is 3.
In some embodiments of the compounds of Formula (I), at least one of R′, R₃, R₄, R₅and R₆is —(C═O)CH(NH)(CH₂)_n(C═NH)—NH₂and n is 2.
In some embodiments of the compounds of Formula (I), at least one of R′, R₃, R₄, R₅and R₆is
In some embodiments of the compounds of Formula (III), at least one of R′, R₃, R₄, and R₅is —(C═O)(CH₂)_n(NH₂) and n is 3.
In some embodiments of the compounds of Formula (III), at least one of R′, R₃, R₄, and R₅is —(C═O)CH(NH₂)(CH₂)_n(NH₂) and n is 4.
In some embodiments of the compounds of Formula (III), at least one of R′, R₃, R₄, and R₅is —(C═O)(CH₂)_n(NH)(C═NH)—NH₂and n is 3.
In some embodiments of Formula (III), at least one of R′, R₃, R₄, and R₅is —(C═O)CH(NH₂)(CH₂)_n(NH)(C═NH)—NH₂and n is 2.
In some embodiments of Formula (III), at least one of R′, R₃, R₄, and R₅is
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), L and M are independently alkyl or aryl.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), L is alkyl and M is alkyl or aryl.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), L is methyl or isopropyl and M is alkyl or aryl.
In some embodiments of the compounds of Formula (I) or Formula (II), W is oxygen, sulfur or NR₆;
In some embodiments of the compounds of Formula (I) or Formula (II), W is NR₆and R₆is hydrogen or alkyl.
In some embodiments of the compounds of Formula (I) or Formula (II), R₇, R₈, and R₉are independently hydrogen or alkyl.
In some embodiments of the compounds of Formula (III), R₈, and R₉are independently hydrogen or alkyl.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), R₈is hydrogen.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), R₈is alkyl.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), R₈is C₁-C₆alkyl.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), R₉is hydrogen, halogen, or alkyl.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), R₉is hydrogen.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), R₉is alkyl.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), R₉is C₁-C₆alkyl.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), R₉is halogen. In some embodiments, R₉is —F, —Cl, —Br, or —I. In some embodiments, R₉is —Cl, —Br, or —I. In some embodiments, R₉is —Cl or —Br. In some embodiments, R₉is —F. In some embodiments, R₉is —Cl. In some embodiments, R₉is —Br. In some embodiments, R₉is —I.
In some embodiments of the compounds of Formula (I) or Formula (II), R₇and R₈are independently hydrogen or C₁-C₈alkyl optionally substituted with 1-3 groups independently selected from hydroxyl, thiol and halogen.
In some embodiments of the compounds of Formula (III), R₈is hydrogen or C₁-C₈alkyl optionally substituted with 1-3 groups independently selected from hydroxyl, thiol and halogen.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), R₉is hydrogen, halogen, or C₁-C₈alkyl optionally substituted with 1-3 groups independently selected from hydroxyl, thiol and halogen.
In some embodiments of the compounds of Formula (I) or Formula (II), R₇, R₈and R₉are independently hydrogen or C₁-C₈alkyl optionally substituted with 1-3 groups independently selected from hydroxyl, thiol and halogen.
In some embodiments of the compounds of Formula (III), R₈and R₉are independently hydrogen or C₁-C₈alkyl optionally substituted with 1-3 groups independently selected from hydroxyl, thiol and halogen.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), n is an integer from 2-7 (i.e. 2, 3, 4, 5, 6, or 7). In some embodiments n is 2. In some embodiments, n is 3. In some embodiments, n is 4. In some embodiments, n is 5. In some embodiments, n is 6. In some embodiments, n is 7.
In some embodiments of the compounds of Formula (I), Formula (II), or Formula (III), m is an integer from 1-4 (i.e. 1, 2, 3, or 4). In some embodiments, m is 1. In some embodiments, m is 2. In some embodiments, m is 3. In some embodiments, m is 4.
In some embodiments of the compounds of Formula (I), the compound is of Formula (IV):
wherein R₁₀is —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or
A is a pharmaceutically acceptable anion,
and wherein R₁, R₂, R₃, R₄, R₅, R₈, R₉, X, Y, Z, W, and m are defined herein.
In some embodiments, the compound of Formula (I) is a compound of Formula (V):
wherein R₁₀is —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH)(C═NH)—NH₂, —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or
A is a pharmaceutically acceptable anion,
and wherein R₁, R₂, R₃, R₄, R₅, R₈, R₉, X, Y, Z, and m are defined herein.
In some embodiments, the present disclosure provides compounds of Formula (VI):
wherein R₁, R₂, R₃, R₄, and R₅are as defined herein,
X is carbon or nitrogen;
W is carbon, oxygen, or nitrogen; and
Z is hydrogen, deuterium, or oxygen.
In some embodiments, the compound of Formula (VII) is:
In some embodiments, the compound of Formula (VI) or (VII) is:
In some embodiments, the present disclosure provides compounds of Formula (VIII):
wherein R₁, R₂, R₃, R₄, R₅, and R₉, are as defined herein,
R₁₁and R₁₂are independently a pharmaceutically acceptable salt, hydrogen, deuterium, alkyl, substituted alkyl, cycloalkyl, or R₁₁and R₁₂together with the atoms to which they are attached form an alkyl, cycloalkyl, heterocycles and are ether, hemiaminal ketal and amino acid.
In some embodiments of the compounds of Formula (VIII), R₁₁and R₁₂together with the atoms to which they are attached form a heterocycle.
In some embodiments of the compounds of Formula (VIII), the pharmaceutically acceptable salt is Na⁺, K⁺, or NH₄ ⁺.
In some embodiments, provided herein is a compound of Formula (IX):
or a pharmaceutically acceptable salt thereof;
wherein,
R₁and R₂are each independently hydrogen, alkyl, —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH)(C═NH)—NH₂, —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or
X, Y and Z are each independently hydrogen, halogen, —O—, —S—, —NR_A—, or —O(P═O)—OR_A(—OR′), wherein R′ and R_Ais hydrogen or alkyl;
R₃, R₄, R₅and R₆are each independently absent, hydrogen, or alkyl;
R₇and R₈are each independently hydrogen or alkyl;
R₉is hydrogen, alkyl, or halogen;
L and M are each independently alkyl or aryl;
W is oxygen, sulfur or NR₆;
n is an integer from 2-7; and
m is an integer from 1-4,
wherein at least one of R₁and R₂is —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH)(C═NH)—NH₂, —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or
In some embodiments of the compounds of Formula (IX), X, Y and Z are each independently hydrogen, halogen, —O—, —S—, —NR_A—, or —O(P═O)—OR_A(—OR′).
In some embodiments of the compounds of Formula (IX), X, Y and Z are hydrogen.
In some embodiments of the compounds of Formula (IX), two of X, Y and Z are hydrogen.
In some embodiments of the compounds of Formula (IX), two of X, Y and Z are hydrogen and the non-hydrogen X, Y or Z is —O—, —S—, —NR_A—, or —O(P═O)—OR_A(—OR′), wherein R_Ais hydrogen or alkyl.
In some embodiments of the compounds of Formula (IX), X and Y are hydrogen; and Z is —O—, —S—, —NR_A—, or —O(P═O)—OR_A(—OR′), wherein R_Ais hydrogen or alkyl.
In some embodiments of the compounds of Formula (IX), X and Z are hydrogen; and Y is —O—, —S—, —NR_A—, or —O(P═O)—OR_A(—OR′), wherein R_Ais hydrogen or alkyl.
In some embodiments of the compounds of Formula (IX), Y and Z are hydrogen; and X is —O—, —S—, —NR_A—, or —O(P═O)—OR_A(—OR′), wherein R_Ais hydrogen or alkyl.
In some embodiments of the compounds of Formula (IX), at least one of X, Y, or Z is —O(P═O)—OR_A(—OR′).
In some embodiments of the compounds of Formula (IX), X is —O(P═O)—OR_A(—OR′) and R₃is absent.
In some embodiments of the compounds of Formula (IX), Y is —O(P═O)—OR_A(—OR′) and R₄is absent.
In some embodiments of the compounds of Formula (IX), Z is —O(P═O)—OR_A(—OR′) and R₅is absent.
In some embodiments of the compounds of Formula (IX), R′ is hydrogen or alkyl.
In some embodiments of the compounds of Formula (IX), R_Ais hydrogen or alkyl.
In some embodiments of the compounds of Formula (IX), R₁and R₂are each independently absent, hydrogen, alkyl, —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH)(C═NH)—NH₂, —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or
wherein at least one of R₁and R₂is —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or
In some embodiments of the compounds of Formula (IX), R₁is absent, hydrogen, alkyl, —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH)(C═NH)—NH₂, —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or
In some embodiments of the compounds of Formula (IX), R¹is hydrogen or alkyl.
In some embodiments of the compounds of Formula (IX), R₂is absent, hydrogen, alkyl, —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH)(C═NH)—NH₂, —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or
In some embodiments of the compounds of Formula (IX), R₂is hydrogen or alkyl.
In some embodiments of the compounds of Formula (IX), at least one of R₁and R₂is —(C═O)(CH₂)_n(NH₂) and n is 3.
In some embodiments of the compounds of Formula (IX), at least one of R₁and R₂is —(C═O)CH(NH₂)(CH₂)_n(NH₂) and n is 4.
In some embodiments of the compounds of Formula (IX), at least one of R₁and R₂is —(C═O)(CH₂)_n(NH)(C═NH)—NH₂and n is 3.
In some embodiments of the compounds of Formula (IX), at least one of R₁and R₂is —(C═O)CH(NH)(CH₂)_n(C═NH)—NH₂and n is 2.
In some embodiments of the compounds of Formula (IX), at least one of R₁and R₂is —(C═O)-alkyl.
In some embodiments of the compounds of Formula (IX), at least one of R₁and R₂is
In some embodiments of the compounds of Formula (IX), at least one of R₁and R₂is —(C═O)CH(NH₂)(CH₂)_n(NH)(C═NH)—NH₂and n is 4.
In some embodiments of the compounds of Formula (IX), at least one of R₁and R₂is —(C═O)CH(NH₂)(CH₂)_n(NH)(C═NH)—NH₂and n is 3.
In some embodiments of the compounds of Formula (IX), L and M are each independently alkyl or aryl.
In some embodiments of the compounds of Formula (IX), L is alkyl and M is alkyl or aryl.
In some embodiments of the compounds of Formula (IX), L is methyl or isopropyl and M is alkyl or aryl.
In some embodiments, R₃is hydrogen.
In some embodiments, R₃is alkyl.
In some embodiments, R₃is C₁-C₆alkyl.
In some embodiments, R₃is absent.
In some embodiments, R₄is hydrogen.
In some embodiments, R₄is alkyl.
In some embodiments, R₄is C₁-C₆alkyl.
In some embodiments, R₄is absent.
In some embodiments, R₅is hydrogen.
In some embodiments, R₅is alkyl.
In some embodiments, R₅is C₁-C₆alkyl.
In some embodiments, R₅is absent.
In some embodiments of the compounds of Formula (IX), W is oxygen, sulfur or NR₆.
In some embodiments of the compounds of Formula (IX), W is NR₆and R₆is hydrogen or alkyl.
In some embodiments of the compounds of Formula (IX), R₇, R₈, and R₉are each independently hydrogen or alkyl.
In some embodiments of the compounds of Formula (IX), R₈is hydrogen.
In some embodiments of the compounds of Formula (IX), R₈is alkyl.
In some embodiments of the compounds of Formula (IX), R₈is C₁-C₆alkyl.
In some embodiments of the compounds of Formula (IX), R₉is hydrogen, halogen, or alkyl.
In some embodiments of the compounds of Formula (IX), R₉is hydrogen.
In some embodiments of the compounds of Formula (IX), R₉is alkyl.
In some embodiments of the compounds of Formula (IX), R₉is C₁-C₆alkyl.
In some embodiments of the compounds of Formula (IX), R₉is halogen. In some embodiments, R₉is —F, —Cl, —Br, or —I. In some embodiments, R₉is —Cl, —Br, or —I. In some embodiments, R₉is —Cl or —Br. In some embodiments, R₉is —F. In some embodiments, R₉is —Cl. In some embodiments, R₉is —Br. In some embodiments, R₉is —I.
In some embodiments of the compounds of Formula (IX), R₇and R₈are each independently hydrogen or C₁-C₈alkyl optionally substituted with 1-3 groups independently selected from hydroxyl, thiol and halogen.
In some embodiments of the compounds of Formula (IX), R₉is hydrogen, halogen, or C₁-C₈alkyl optionally substituted with 1-3 groups independently selected from hydroxyl, thiol and halogen.
In some embodiments of the compounds of Formula (IX), R₇, R₈and R₉are each independently hydrogen or C₁-C₈alkyl optionally substituted with 1-3 groups independently selected from hydroxyl, thiol and halogen.
In some embodiments of the compounds of Formula (IX), n is an integer from 2-7 (i.e. 2, 3, 4, 5, 6, or 7). In some embodiments n is 2. In some embodiments, n is 3. In some embodiments, n is 4. In some embodiments, n is 5. In some embodiments, n is 6. In some embodiments, n is 7.
In some embodiments of the compounds of Formula (IX), m is an integer from 1-4 (i.e. 1, 2, 3, or 4). In some embodiments, m is 1. In some embodiments, m is 2. In some embodiments, m is 3. In some embodiments, m is 4.
In some embodiments, provided herein is a compound of Formula (X):
wherein
R₁and R₂together with the atoms to which they are attached form a heterocycle;
X and Y are each independently hydrogen, halogen, —O—, —S—, —NR_A—, or —O(P═O)—OR_A(—OR′), wherein R_Ais hydrogen or alkyl; and
R′, R₃and R₄are each independently absent, hydrogen, alkyl, —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)CH(NH₂) (CH₂)_n(NH)(C═NH)—NH₂, —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or
wherein at least one of R′, R₃, and R₄is —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or
In some embodiments, provided herein is one or more compounds selected from Table 1.
In some embodiments, provided herein is one or more pharmaceutically acceptable salts of a compound selected from Table 1.

TABLE 1

Compounds

No.	Structure

1-1

1-2

1-3

1-4

1-5

1-6

1-7

1-8

1-9

1-10

1-11

1-12

1-13

In some embodiments, provided herein is one or more compounds selected from Table 2.
In some embodiments, provided herein is one or more pharmaceutically acceptable salts of a compound selected from Table 2.

TABLE 2

Compounds

No.	Structure

2-1

2-2

2-3

2-4

2-5

2-6

2-7

2-8

2-9

2-10

2-11

2-12

2-13

In some embodiments of the compounds of Table 2, X and Y are hydrogen and R₃and R₄are absent.
In some embodiments of the compounds of Table 2, X is hydrogen, R₃is absent, Y is O, and R₄is hydrogen.
In some embodiments of the compounds of Table 2, X is hydrogen, R₃is absent, Y is O, and R₄is Me.
In some embodiments of the compounds of Table 2, X is hydrogen, R₃is absent, Y is O, and R₄is Ac.
In some embodiments of the compounds of Table 2, X is O, R₃is hydrogen, Y is hydrogen, and R₄is absent.
In some embodiments of the compounds of Table 2, X is O, R₃is Me, Y is hydrogen, and R₄is absent.
In some embodiments of the compounds of Table 2, X is O, R₃is Ac, Y is hydrogen, and R₄is absent. In some embodiments, provided herein is one or more compounds selected from Table 3.
In some embodiments, provided herein is one or more pharmaceutically acceptable salts of a compound selected from Table 3.

TABLE 3

Compounds

No.	Structure

3-1

3-2

3-3

3-4

wherein X, Y, R₃and R₄have the definitions provided herein.

In some embodiments of the compounds of Table 3, R₃is absent, X is hydrogen, R₄is absent, and Y is hydrogen.
In some embodiments of the compounds of Table 3, R₃is absent, X is hydrogen, R₄is hydrogen, and Y is —O—.
In some embodiments of the compounds of Table 3, R₃is absent, X is hydrogen, R₄is alkyl, and Y is —O—.
In some embodiments of the compounds of Table 3, R₃is hydrogen, X is —O—, R₄is absent hydrogen, and Y is hydrogen.
In some embodiments of the compounds of Table 3, R₃is alkyl, X is —O—, R₄is absent hydrogen, and Y is hydrogen.
In some embodiments of the compounds of Table 3, R₃is absent, X is hydrogen, R₄is absent, Y is —O(P═O)(OR_A)(OR′), R_Ais hydrogen or alkyl, and R′ is hydrogen or alkyl.
In some embodiments of the compounds of Table 3, R₃is absent, X is hydrogen, R₄is absent, and Y is —O(P═O)(OH)(OH).
In some embodiments of the compounds of Table 3, R₃is absent, X is —O(P═O)(OR_A)(OR′), R_Ais hydrogen or alkyl, R′ is hydrogen or alkyl, R₄is absent, and Y is hydrogen.
In some embodiments of the compounds of Table 3, R₃is absent, X is —O(P═O)(OH)(OH), R₄is absent, and Y is hydrogen.
In some embodiments, provided herein is one or more compounds selected from Table 4.
In some embodiments, provided herein is one or more pharmaceutically acceptable salts of a compound selected from Table 4.

TABLE 4

Compounds

	1-2-1

		1-2-1

	1-2-2

		1-2-2

	1-2-3

		1-2-3

	1-2-4

		1-2-4

	1-2-5

		1-2-5

	1-2-6

		1-2-6

	1-2-7

		1-2-7

	1-2-8

		1-2-8

	1-2-9

		1-2-9

	1-2-10

		1-2-10

	1-2-11

		1-2-11

	1-2-12

		1-2-12

	1-2-13

		1-2-13

	1-2-14

		1-2-14

	2-1-2

		2-1-2

	3-1-2

		3-1-2

	3-1-3

		3-1-3

	3-1-4

		3-1-4

	4-1-1

		4-1-1

	4-1-2

		4-1-2

	4-1-3

		4-1-3

	4-1-4

		4-1-4

	5-1-1

		5-1-1

	5-1-2

		5-1-2

	5-1-3

		5-1-3

	5-1-4

		5-1-4

	6-1-1

		6-1-1

	6-1-2

		6-1-2

	6-1-3

		6-1-3

Compositions

In some embodiments of the present disclosure, a pharmaceutical composition comprises a therapeutically effective amounts of one or more compounds of the present disclosure (e.g., a compound of Formulas (I)-(X) or Compounds of Tables 1-4) or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable excipient.
The pharmaceutically acceptable excipients and adjuvants are added to the composition or formulation for a variety of purposes. In some embodiments, a pharmaceutical composition comprising one or more compounds disclosed herein, or a pharmaceutically acceptable salt thereof, further comprise a pharmaceutically acceptable carrier. In some embodiments, a pharmaceutically acceptable carrier includes a pharmaceutically acceptable excipient, binder, and/or diluent. In some embodiments, suitable pharmaceutically acceptable carriers include, but are not limited to, inert solid fillers or diluents and sterile aqueous or organic solutions. In some embodiments, suitable pharmaceutically acceptable excipients include, but are not limited to, water, salt solutions, alcohol, polyethylene glycols, gelatin, lactose, amylase, magnesium stearate, talc, silicic acid, viscous paraffin, and the like.
For the purposes of this disclosure, the compounds of the present disclosure can be formulated for administration by a variety of means including orally, parenterally, by inhalation spray, topically, or rectally in formulations containing pharmaceutically acceptable carriers, adjuvants and vehicles. The term parenteral as used here includes subcutaneous, intravenous, intramuscular, and intraarterial injections with a variety of infusion techniques. Intraarterial and intravenous injection as used herein includes administration through catheters.

Methods

In one aspect, the present disclosure provides methods of treating or preventing neurological disorders in a subject in need thereof, the methods comprising administering a therapeutically effective amount of a compound disclosed herein (e.g., a compound of Formulas (I)-(X) or Compounds of Tables 1-4), a pharmaceutically acceptable salt thereof, or a pharmaceutical composition thereof to the subject.
In some embodiments, the neurological disorder is a mood disorder. In some embodiments, the mood disorder is clinical depression, postnatal depression or postpartum depression, perinatal depression, atypical depression, melancholic depression, psychotic major depression, cationic depression, seasonal affective disorder, dysthymia, double depression, depressive personality disorder, recurrent brief depression, major depressive disorder, minor depressive disorder, bipolar disorder or manic depressive disorder, depression caused by chronic medical conditions, treatment-resistant depression, refractory depression, suicidality, suicidal ideation, or suicidal behavior. In some embodiments, the method described herein provides therapeutic effect to a subject suffering from depression (e.g., moderate or severe depression). In some embodiments, the mood disorder is associated with neuroendocrine diseases and disorders, neurodegenerative diseases and disorders (e.g., epilepsy), movement disorders, tremor (e.g., Parkinson's Disease), or women's health disorders or conditions. In some embodiments, the mood disorder is depression. In some embodiments, the mood disorder is treatment-resistant depression or major depressive disorder. In some embodiments, the mood disorder is major depressive disorder. In some embodiments, the mood disorder is treatment-resistant depression.
In some embodiments, the present disclosure provides methods of treating or preventing PTSD, mood disorders, general anxiety disorder, addictive disorders, and/or drug dependence in a subject in need thereof, the methods comprising administering a therapeutically effective amount of a compound disclosed herein (e.g., a compound of Formulas (I)-(X) or Compounds of Tables 1-4), a pharmaceutically acceptable salt thereof, or a pharmaceutical composition thereof to the subject.
In some embodiments, the present disclosure provides methods of treating or preventing PTSD in a subject in need thereof, the methods comprising administering a therapeutically effective amount of a compound disclosed herein (e.g., a compound of Formulas (I)-(X) or Compounds of Tables 1-4), a pharmaceutically acceptable salt thereof, or a pharmaceutical composition thereof to the subject.
In some embodiments, the methods include treating PTSD through induction and maintenance therapy by administering a compound disclosed herein, a pharmaceutically acceptable salt thereof, or a pharmaceutical composition thereof. In some embodiments, the compounds of the present disclosure are used for induction and maintenance therapy to treat PTSD with an improved safety profile when compared to treatment with the entactogenic, oneirophrenic or psychedelic compound (e.g., dimethyltryptamine or related compound, psilocybin, or MDMA) alone.
In some embodiments, the present disclosure provides methods of treating or preventing behavioral or mood disorders in a subject in need thereof, the methods comprising administering a therapeutically effective amount of a compound disclosed herein (e.g., a compound of Formulas (I)-(X) or Compounds of Tables 1-4), a pharmaceutically acceptable salt thereof, or a pharmaceutical composition thereof to the subject. In some embodiments, the behavioral or mood disorder includes anxiety, such as social anxiety in autistic subjects (e.g., autistic adults) and anxiety related to life-threatening illnesses. In some embodiments, the behavioral or mood disorder includes stress (where moderation thereof is measured, e.g., by effects on amygdala responses). In some embodiments, the anxiety disorder is panic disorder, obsessive-compulsive disorder, and/or general anxiety disorder. In some embodiments, the subject suffers from a lack of motivation, attention, lack of accuracy in memory recall, speed of response, perseveration, and/or cognitive engagement. Further examples include depression (e.g., MDD or TRD), attention disorders, disorders of executive function and/or cognitive engagement, obsessive compulsive disorder, bipolar disorder, panic disorder, phobia, schizophrenia, psychopathy, antisocial personality disorder and/or neurocognitive disorders.
In some embodiments, the present disclosure provides methods for treating an addictive disorder in a subject in need thereof, the methods comprising administering a therapeutically effective amount of a compound disclosed herein (e.g., a compound of Formulas (I)-(X) or Compounds of Tables 1-4), a pharmaceutically acceptable salt thereof, or a pharmaceutical composition thereof to the subject. In some embodiments, the addictive disorder is alcohol abuse, substance abuse, smoking, obesity, or mixtures thereof. In some embodiments, the disorder is an eating disorder (e.g., anorexia nervosa, bulimia, nervosa, binge eating disorder, etc.) or an auditory disorder.
In some embodiments, the present disclosure provides methods for treating an impulsive disorder in a subject in need thereof, the methods comprising administering a therapeutically effective amount of a compound disclosed herein (e.g., a compound of Formulas (I)-(X) or Compounds of Tables 1-4), a pharmaceutically acceptable salt thereof, or a pharmaceutical composition thereof to the subject. In some embodiments, the impulsive disorder is attention deficit hyperactivity disorder (ADHD), attention deficit disorder (ADD), Tourette's syndrome, autism, or combinations thereof.
In some embodiments, the present disclosure provides methods for treating a compulsive disorder in a subject in need thereof, the methods comprising administering a therapeutically effective amount of a compound disclosed herein (e.g., a compound of Formulas (I)-(X) or Compounds of Tables 1-4), a pharmaceutically acceptable salt thereof, or a pharmaceutical composition thereof to the subject. In some embodiments, the compulsive disorder is obsessive compulsive disorder (OCD), gambling, aberrant sexual behavior, or combinations thereof.
In some embodiments, the present disclosure provides methods for treating a personality disorder in a subject in need thereof, the methods comprising administering a therapeutically effective amount of a compound disclosed herein (e.g., a compound of Formulas (I)-(X) or Compounds of Tables 1-4), a pharmaceutically acceptable salt thereof, or a pharmaceutical composition thereof. In some embodiments, the personality disorder is conduct disorder, antisocial personality, aggressive behavior, or combinations thereof to the subject.

Examples

Compounds of the present disclosure can be synthesized using the following exemplary methods or other methods that are known to those skilled in the art.
General reaction conditions are provided, and reaction products can be purified by known methods including silica gel chromatography using various organic solvents such as hexane, dichloromethane, ethyl acetate, methanol and the like or preparative reverse phase high pressure liquid chromatography.
Preparation of compounds can involve the protection and deprotection of various chemical groups. The need for protection and deprotection, and the selection of appropriate protecting groups can be readily determined by one skilled in the art. The chemistry of protecting groups can be found, for example, in Greene and Wuts, Protective Groups in Organic Synthesis, 44th. Ed., Wiley & Sons, 2006, as well as in Jerry March, Advanced Organic Chemistry, 4^thedition, John Wiley & Sons, publisher, New York, 1992 which are incorporated herein by reference in their entirety.
As shown in Scheme 1, 2-(1H-indol-3-yl)-N,N-dimethylethan-1-amine can be coupled with a carboxylic acid of Formula (A) where PG₁is a protecting group (such as Boc) under amide coupling conditions (e.g., in the presence of 1,1′-carbonyldiimidazole (CDI), dimethylaminopyridine (DMAP) and a strong base, such as sodium hydride), followed by deprotection of the protecting group PG₁(e.g., using TFA) to provide compounds of Formula (I-a).
As shown in Scheme 2, 2-(1H-indol-3-yl)-N-methylethan-1-amine can be reacted with a carboxylic acid of Formula (A) under amide coupling conditions (e.g., in the presence of CDI, DMAP and a base, such as a tertiary amine base like triethylamine) where PG₁is a protecting group (such as Boc) followed by deprotection of the protecting group PG₁(e.g., using TFA) to provide compounds of Formula (I-b).
As shown in Scheme 3, 3-(2-(dimethylamino)ethyl)-1H-indol-4-ol can be converted into 3-(2-(dimethylamino)ethyl)-1H-indol-4-yl dihydrogen phosphate in the presence of POCl₃. 3-(2-(dimethylamino)ethyl)-1H-indol-4-yl dihydrogen phosphate can be reacted with a carboxylic acid of Formula (B) in the presence of CDI and a tertiary amine such as triethylamine to form a compound of Formula (I-c). Compounds of Formula (I-c) can be deprotected e.g., with TFA to provide compounds of Formula (I-d).
1. Synthesis of 1-2
To a solution of tryptophol (6 g, 37.27 mmol, 1.0 equiv) in dimethyl sulfoxide (60 mL) was added 2-Iodoxybenzoic acid (12.5 g, 44.72 mmol, 1.2 equiv) at 40° C. under nitrogen atmosphere. The mixture solution was stirred for 2 h at 40° C. The reacting solution was diluted with 200 mL of dichloromethane, washed with 2×200 mL saturated sodium bicarbonate solution and 2×200 mL saturated aqueous sodium chloride respectively. The organic layer was dried over anhydrous sodium sulfate, filtered and concentrated under vacuum. The residue was applied onto a silica gel column with petroleum ether/ethyl acetate (3:1). 4 g (67%) of 1-2 was obtained as a yellow oil.
2. Synthesis of 1-2-1
To a solution of 1-2 (100 mg, 0.63 mmol, 1.00 equiv) and 1-Isopropylpiperazine (241.5 mg, 1.89 mmol, 3.00 equiv) in dichloromethane (1 mL) were added triethylamine (190.9 mg, 1.89 mmol, 3.00 equiv) and the solution of SnCl₄in dichloromethane (1N, 0.32 mL, 0.5 equiv) at 25° C. The resulting mixture was stirred for 1 h at 25° C. under nitrogen atmosphere. Then sodium cyanoborohydride (58.6 mg, 0.95 mmol, 1.50 equiv) was added in portions. The mixture was stirred for 2 h at 25° C. The reaction was diluted with 20 mL of dichloromethane. The organic layer was washed with 2×20 mL saturated aqueous sodium chloride solution, dried over anhydrous sodium sulfate, filtered and concentrated under vacuum. The crude product was purified by Prep-HPLC for 2 times with the following condition: Column: XBridge Prep OBD C18 Column, 30*150 mm, 5 m; Mobile Phase A: Water (10 mmol/L NH₄HCO₃+0.1% NH₃.H₂O), Mobile Phase B: ACN; Flow rate: 60 mL/min; Gradient: 22% B to 50% B in 9 min, 50% B; Wave Length: 254 nm; 4.6 mg (3%) of 1-2-1 was obtained as a brown solid. MS m/z [M+H]⁺ (ESI): 272.25. ¹H NMR (300 MHz, DMSO-d6) δ 10.76 (s, 1H), 7.51-7.49 (m, 1H), 7.34-7.32 (m, 1H), 7.14-6.97 (m, 3H), 2.83 (s, 2H), 2.58-2.46 (m, 11H), 0.97 (s, 6H).
3. Synthesis of 1-2-2
In a similar manner described for 1-2-1, compound 1-2-2 was prepared from 100 mg of 1-2 and 189 mg of 1-methylpiperazine. 5.1 mg (3%) of 1-2-2 was obtained as a brown oil. MS m/z [M+H]⁺ (ESI): 244.10. ¹H NMR (300 MHz, DMSO-d6) δ 10.75 (s, 1H), 7.49 (d, J=7.5 Hz, 1H), 7.32 (d, J=8.1 Hz, 1H), 7.13 (s, 1H), 7.05 (t, J=7.5 Hz, 1H), 6.95 (t, J=7.4 Hz, 1H), 2.85-2.79 (m, 2H), 2.58-2.53 (m, 2H), 2.47-2.33 (m, 8H), 2.15 (s, 3H).
4. Synthesis of 1-2-3
In a similar manner described for 1-2-1, compound 1-2-3 was prepared from 100 mg of 1-2 and 164.4 mg of morpholine. 25.5 mg (17%) of 1-2-3 was obtained as a brown solid. MS m/z [M+H]⁺ (ESI): 231.05. ¹H NMR (300 MHz, DMSO-d6) δ 10.77 (s, 1H), 7.50 (d, J=7.8 Hz, 1H), 7.32 (d, J=7.8 Hz, 1H), 7.15 (s, 1H), 7.07-6.94 (m, 2H), 3.60 (t, J=4.2 Hz, 4H), 2.84 (t, J=7.8 Hz, 2H), 2.54 (t, J=7.8 Hz, 2H), 2.47-2.45 (m, 4H).
Synthesis of 1-2-4
In a similar manner described for 1-2-1, compound 1-2-4 was prepared from 100 mg of 1-2 and 168.2 mg of piperidine. 3.4 mg (2%) of 1-2-4 was obtained as a brown solid. MS m/z [M+H]⁺ (ESI): 229.20. ¹H NMR (300 MHz, DMSO-d6) δ 10.75 (s, 1H), 7.49 (d, J=5.1 Hz, 1H), 7.32 (d, J=6.0 Hz, 1H), 7.12 (s, 1H), 7.04-6.95 (m, 2H), 2.82 (s, 2H), 2.55 (s, 2H), 2.42 (s, 4H), 1.51-1.40 (m, 6H).
Synthesis of 1-2-5
In a similar manner described for 1-2-1, compound 1-2-5 was prepared from 100 mg of 1-2 and 190.9 mg of 4-Hydroxypiperidine. 18.9 mg (12%) of 1-2-5 was obtained as a brown solid. MS m/z [M+H]⁺ (ESI): 245.10. ¹H NMR (300 MHz, DMSO-d6) δ 10.75 (s, 1H), 7.49 (d, J=7.8 Hz, 1H), 7.32 (d, J=7.8 Hz, 1H), 7.13-6.93 (m, 3H), 4.53 (s, 1H), 3.49-3.38 (m, 1H), 2.87-2.73 (m, 4H), 2.59-2.52 (m, 2H), 2.12-2.01 (m, 2H), 1.75-1.70 (m, 2H), 1.45-1.33 (m, 2H).
7. Synthesis of 1-2-6
To a solution of methyl 4-piperidineacetate (296.7 mg, 1.89 mmol, 3.00 equiv) and acetic acid (0.1 mL) in methanol (1 mL) were added sodium cyanoborohydride (58.6 mg, 0.95 mmol, 1.50 equiv) at 0° C. Then 1-2 (100 mg, 0.63 mmol, 1.00 equiv) was added to the mixture dropwise at 0° C. The mixture was stirred at 25° C. for 2 h. The reaction was diluted with 20 mL of dichloromethane. The organic layer was washed with 2×20 mL saturated aqueous sodium chloride solution, dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The crude product was purified by Prep-HPLC with the following condition: Column: XBridge Prep OBD C18 Column, 30*150 mm, 5 m; Mobile Phase A: Water (10 mmol/L NH₄HCO₃+0.1% NH₃.H₂O), Mobile Phase B: ACN; Flow rate: 60 mL/min; Gradient: 30% B to 58% B in 9 min, 58% B; Wavelength: 254 nm. 27.1 mg (14%) of 1-2-6 was obtained as a brown solid. MS m/z [M+H]⁺ (ESI): 301.05. ¹H NMR (300 MHz, DMSO-d6) δ 10.75 (s, 1H), 7.49 (d, J=7.8 Hz, 1H), 7.32 (d, J=8.1 Hz, 1H), 7.12 (d, J=1.8 Hz, 1H), 7.07-7.02 (m, 1H), 6.97-6.93 (m, 1H), 3.59 (s, 3H), 2.96-2.92 (m, 2H), 2.84-2.79 (m, 2H), 2.57-2.51 (m, 2H), 2.24 (d, J=6.6 Hz, 2H), 1.98-1.89 (m, 2H), 1.72-1.60 (m, 3H), 1.29-1.13 (m, 2H).
8. Synthesis of 1-2-7
In a similar manner described for 1-2-1, compound 1-2-7 was prepared from 100 mg of 1-2 and 270.2 mg of piperidin-4-ylacetic acid. 7.8 mg (4%) of 1-2-7 was obtained as a white solid. MS m/z [M+H]⁺ (ESI): 287.10. ¹H NMR (300 MHz, DMSO-d6) δ 10.76 (s, 1H), 7.50 (d, J=7.5 Hz, 1H), 7.33 (d, J=7.4 Hz, 1H), 7.13-6.95 (m, 3H), 3.02-2.89 (m, 2H), 2.88-2.74 (m, 2H), 2.57-2.53 (m, 2H), 2.11 (s, 2H), 1.99-1.90 (m, 2H), 1.67-1.63 (m, 3H), 1.26-1.12 (m, 2H).
9. Synthesis of 1-2-8
In a similar manner described for 1-2-7, compound 1-2-8 was prepared from 100 mg of 1-2 and 270.2 mg of methyl isonipecotate. 26.1 mg (14%) of 1-2-8 was obtained as a brown solid. MS m/z [M+H]⁺ (ESI): 287.05. ¹H NMR (300 MHz, DMSO-d6) δ10.76 (s, 1H), 7.49 (d, J=7.8 Hz, 1H), 7.34-7.29 (m, 1H), 7.13 (d, J=2.1 Hz, 1H), 7.07-7.02 (m, 1H), 6.98-6.93 (m, 1H), 3.60 (s, 3H), 2.94-2.89 (m, 2H), 2.85-2.80 (m, 2H), 2.58-2.53 (m, 2H), 2.38-2.26 (m, 1H), 2.09-1.99 (m, 2H), 1.86-1.77 (m, 2H), 1.64-1.51 (m, 2H).
10. Synthesis of 1-2-9
In a similar manner described for 1-2-1, compound 1-2-9 was prepared from 100 mg of 1-2 and 243.8 mg of isonipecotic acid. 4.3 mg (2%) of 1-2-9 was obtained as a white solid. MS m/z [M+H]⁺ (ESI): 273.10. ¹H NMR (300 MHz, DMSO-d6) δ 10.75 (s, 1H), 7.49 (d, J=7.2 Hz, 1H), 7.31 (d, J=7.5 Hz, 1H), 7.13 (s, 1H), 7.09-6.90 (m, 2H), 2.95-2.78 (m, 4H), 2.58-2.53 (m, 2H), 2.22-1.96 (m, 3H), 1.82-1.77 (m, 2H), 1.61-1.48 (m, 2H).
11. Synthesis of 1-2-10
In a similar manner described for 1-2-1, compound 1-2-10 was prepared from 100 mg of 1-2 and 134.2 mg of pyrrolidine. 8.0 mg (6%) of 1-2-10 was obtained as a brown solid. MS m/z [M+H]⁺ (ESI): 215.10. ¹H NMR (300 MHz, DMSO-d6) δ 10.76 (s, 1H), 7.49 (d, J=7.8 Hz, 1H), 7.32 (d, J=8.1 Hz, 1H), 7.13 (d, J=2.4 Hz, 1H), 7.07-7.02 (m, 1H), 6.98-6.93 (m, 1H), 2.87-2.82 (m, 2H), 2.69-2.64 (m, 2H), 2.59-2.50 (m, 4H), 1.75-1.64 (m, 4H).
12. Synthesis of 1-2-11
In a similar manner described for 1-2-1, compound 1-2-11 was prepared from 100 mg of 1-2 and 164.4 mg of 3-Pyrrolidinol. 5.5 mg (4%) of 1-2-11 (racemate) was obtained as a white solid. MS m/z [M+H]⁺ (ESI): 231.05. ¹H NMR (300 MHz, DMSO-d6) δ 10.75 (s, 1H), 7.49 (d, J=7.8 Hz, 1H), 7.32 (d, J=7.8 Hz, 1H), 7.13 (s, 1H), 7.07-7.02 (m, 1H), 6.98-6.93 (m, 1H), 4.67 (s, 1H), 4.19 (s, 1H), 2.84-2.73 (m, 3H), 2.67-2.58 (m, 3H), 2.45-2.34 (m, 2H), 2.01-1.95 (m, 1H), 1.56-1.51 (m, 1H).
13. Synthesis of 1-2-12
In a similar manner described for 1-2-6, compound 1-2-12 was prepared from 100 mg of 1-2 and 243.8 mg of methyl 3-pyrrolidinecarboxylate. 34.1 mg (20%) of 1-2-12 was obtained as a brown solid. MS m/z [M+H]⁺ (ESI): 273.05. ¹H NMR (300 MHz, DMSO-d6) δ 10.77 (s, 1H), 7.50 (d, J=7.8 Hz, 1H), 7.32 (d, J=8.1 Hz, 1H), 7.14 (d, J=2.1 Hz, 1H), 7.07-7.02 (m, 1H), 6.98-6.93 (m, 1H), 3.62 (s, 3H), 3.08-2.98 (m, 1H), 2.86-2.79 (m, 3H), 2.72-2.53 (m, 5H), 2.04-1.91 (m, 2H).
14 Synthesis of 1-2-13
In a similar manner described for 1-2-1, compound 1-2-13 was prepared from 100 mg of 1-2 and 217.3 mg of methyl 3-Pyrrolidinecarboxylic acid. 24.0 mg (15%) of 1-2-13 was obtained as a white solid. MS m/z [M+H]⁺ (ESI): 259.05. ¹H NMR (300 MHz, DMSO-d6) δ 10.80 (s, 1H), 7.50 (d, J=6.9 Hz, 1H), 7.32 (d, J=7.5 Hz, 1H), 7.14 (s, 1H), 7.08-6.93 (m, 2H), 2.84-2.55 (m, 9H), 1.95 (s, 2H).
15. Synthesis of 1-2-14
In a similar manner described for 1-2-1, compound 1-2-14 was prepared from 100 mg of 1-2 and 228.7 mg of N-Ethylaniline. 6.3 mg (4%) of 1-2-14 was obtained as a white solid. MS m/z [M+H]⁺ (ESI): 265.10. ¹H NMR (300 MHz, DMSO-d6) δ 10.84 (s, 1H), 7.55 (d, J=6.6 Hz, 1H), 7.35 (d, J=7.2 Hz, 1H), 7.22-7.15 (m, 3H), 7.11-6.96 (m, 2H), 6.72 (d, J=6.9 Hz, 2H), 6.60-6.54 (m, 1H), 3.55-3.49 (m, 2H), 3.41-3.35 (m, 2H), 2.95-2.90 (m, 2H), 1.11-1.06 (m, 3H).
16. Synthesis of 2-1-2
To a solution of tryptamine (200 mg, 1.25 mmol, 1.0 equiv) in 3 mL of methanol was added sodium cyanoborohydride (196.9 mg, 3.13 mmol, 2.50 equiv), acetic acid (0.15 mL) and formaldehyde (253.8 mg, 3.13 mol, 2.5 equiv, 37% in aq. soln) in methanol (1.5 mL) at 0° C. The mixture solution was stirred for overnight at 25° C. and then concentrated under vacuum. The resulting solution was diluted with 20 mL of saturated sodium bicarbonate and extracted with 3×20 mL of ethyl acetate. The combined organic layer was washed with 2×20 mL of brine, dried over anhydrous sodium sulfate and concentrated under vacuum. The resulting mixture was dissolved in 2 mL of acetonitrile, then di-tert-butyl (chloromethyl) phosphate (273.5 mg, 1.06 mmol, 2.0 equiv), Me₅-piperidine (164.5 mg, 1.06 mmol, 2.0 equiv) and sodium iodide (8.0 mg, 0.05 mmol, 0.1 equiv) was added at 25° C. under nitrogen atmosphere. The mixture was stirred for 24 h at 40° C. The resulting solution was diluted with 20 mL of ethyl acetate and washed with 2×10 mL of saturated sodium bicarbonate solution and 10 mL of saturated sodium chloride solution respectively. The combined organic layer was washed with 2×20 mL of brine, dried over anhydrous sodium sulfate and concentrated under vacuum. The crude product was used in the next step directly without further purification. 120 mg of 2-1-1 an intermediate phosphate was obtained as a white solid, which was used directly for the next reaction without further characterization.
To a solution of 2-1-1 (120 mg, 0.29 mmol, 1.0 equiv) in dichloromethane (1.2 mL) with an inert atmosphere of argon was added trifluoroacetic acid (0.6 mL) slowly at room temperature. The resulting solution was stirred for 1 h at room temperature. The resulting solution was concentrated under reduced pressure. The crude product was purified by Flash with the following conditions: Column, C18 silica gel; mobile layer, water and methanol (20% methanol up to 100% in 15 min and hold 100% for 5 min); Detector, 220 nm. The fractions were concentrated under reduced pressure. 28.3 mg (27.6% yield) of 2-1-2 was obtained as a white solid. MS m/z [M+H]+(ESI): 299.05. ¹H NMR (300 MHz, DMSO-d6) δ 11.11 (s, 1H), 7.65 (d, J=7.8 Hz, 1H), 7.37 (d, J=8.1 Hz, 1H), 7.26 (s, 1H), 7.11-6.98 (m, 2H), 4.89 (d, J=8.7 Hz, 2H), 3.51-3.45 (m, 2H), 3.18-3.13 (m, 2H), 3.07 (s, 6H). ³¹P NMR (121 MHz, DMSO-d6) δ −1.88.
19. Synthesis of 3-1-4
In a 250 mL flask in the ice bath, 12.83 g (0.11 mol, 1.1 equiv) of thionylchloride was added and then 8.91 g (0.10 mol, 1 equiv) of N,N-dimethyl ethanolamine was added dropwise over 0.5 h under stirring. The resulting mixture was stirred for 1 h at 50° C. Then 150 mL of ethanol was added and heated to reflux for 0.5 h, After cooling to room temperature, 3-1-1 (6 g, 42%) was collected by filtration as a light-yellow solid.
21. Synthesis of 3-1-2
A solution of 3-1-1 (6 g, 41.9 mmol, 1 equiv) in N,N-dimethylformamide (60 mL) was treated with KOH (11.75 g, 209.5 mmol, 5 equiv) for 1 h at 25° C. 7-methoxy-1H-indole (6.17 g, 41.9 mmol, 1 equiv) was added to the mixture at 25° C. The resulting mixture was stirred for 16 h at 50° C. The reaction was diluted with dichloromethane (200 mL), washed with water (3×200 mL). The organic phase was dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The crude product was purified by Flash with the following condition: Column, C18 silica gel; mobile layer, water (0.05% NH₄HCO₃) and acetonitrile (10% acetonitrile up to 100% in 20 min and hold 100% for 5 min); Detector, 220 nm. The fractions were concentrated under reduced pressure to afford 3-1-2 (5 g, 54%) as a white solid. MS m/z [M+H]⁺ (ESI): 219.14.
22. Synthesis of 3-1-3
To a solution of 3-1-2 (600 mg, 2.75 mmol, 1 equiv) in dichloromethane (20 mL) was added aluminium chloride (1.83 g, 13.75 mmol, 5 equiv) in portions at 0° C. The mixture was stirred overnight at 25° C. The reaction was quenched with methanol (20 mL), filtered and concentrated under reduced pressure. The crude product was purified by Flash with the following condition: Column, C18 silica gel; mobile layer, water (0.1% NH₃.H₂O) and methanol (10% methanol up to 100% in 20 min and hold 100% for 5 min); Detector, 220 nm. The fractions were concentrated under reduced pressure. This resulted in 3-1-3 (415 mg, 73%) as a brown oil. MS m/z [M+H]⁺ (ESI): 205.13.
23. Synthesis of 3-1-4
To a solution of 3-1-3 (100 mg, 0.49 mmol, 1 equiv) in dichloromethane (1 mL) was added pentanoic acid (75 mg, 0.74 mmol, 1.5 equiv), 4-dimethylaminopyridine (17.9 mg, 0.15 mmol, 0.3 equiv), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (141.2 mg, 0.74 mmol, 1.5 equiv) at 25° C. The mixture was stirred for 2 h at 25° C. then diluted with 20 mL of dichloromethane, washed with 2×10 mL of saturated sodium bicarbonate solution and 2×10 mL of saturated aqueous sodium chloride respectively. The organic layer was dried over anhydrous sodium sulfate, filtered and concentrated under vacuum. The crude product was purified by Flash with the following condition: Column, C18 silica gel; mobile layer, water (0.05% NH₄HCO₃) and acetonitrile (20% acetonitrile up to 100% in 20 min and hold 100% for 5 min); Detector, 220 nm. The fractions were concentrated under reduced pressure. This resulted in 3-1-4 (22.6 mg, 16%) as a brown oil. MS m/z [M+H]⁺ (ESI): 289.25. ¹H NMR (300 MHz, Methanol-d4) δ 7.42-7.38 (m, 1H), 7.16 (s, 1H), 7.01-6.94 (m, 1H), 6.82-6.78 (m, 1H), 6.48-6.44 (m, 1H), 4.33-4.26 (m, 2H), 2.75-2.60 (m, 4H), 2.23 (s, 6H), 1.81-1.69 (m, 2H), 1.53-1.43 (m, 2H), 1.03-0.96 (m, 3H).
24. Synthesis of 4-1-2, 4-1-3, and 4-1-4
To a solution of tryptamine (400 mg, 2.5 mmol, 1.0 equiv) in 3 mL of methanol was added sodium cyanoborohydride (393.8 mg, 6.26 mmol, 2.50 equiv), acetic acid (0.3 mL) and formaldehyde (507.6 mg, 6.26 mol, 2.5 equiv, 37% in aq. soln) in methanol (4 mL) at 0° C. The mixture solution was stirred for overnight at 25° C. and then concentrated under vacuum. The resulting solution was diluted with 20 mL of saturated sodium bicarbonate and extracted with 3×20 mL of ethyl acetate. The combined organic layer was washed with 2×20 mL of brine, dried over anhydrous sodium sulfate and concentrated under vacuum. The resulting mixture dissolved in 3 mL of dichloromethane, then 483.5 mg of triethylamine was added, 1-chloroethyl carbonochloridate (340 mg, 2.39 mmol, 1.5 equiv) dropwise at 0° C. The mixture was stirred at 25° C. for 2 h. The reaction was diluted with dichloromethane (20 mL), washed with saturated aqueous sodium chloride (2×20 mL). The organic layer was dried over anhydrous sodium sulfate, filtered and concentrated under vacuum. 300 mg (crude) of 4-1-1 (intermediate) was obtained as a yellow solid. It was used for next step directly without further purification.
25. Synthesis of 4-1-2 (racemate)
To a solution of 4-1-1 (300 mg, 1.02 mmol, 1.0 equiv) and triethylamine (2.06 g, 20.40 mmol, 20 equiv) in dimethylformamide (6 mL) was added isobutyric acid (449 mg, 5.10 mmol, 5 equiv) at 25° C. The mixture was stirred for 16 h at 60° C. The reaction was diluted with 50 mL of dichloromethane, washed with 1×50 mL saturated sodium chloride aqueous solution. The organic layer was dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The crude product was purified by Prep-HPLC for 2 times with the following condition: Column: X Select CSH Fluoro Phenyl, 30*150 mm, 5 μm; Mobile Phase A: Water (0.05% HCl), Mobile Phase B: ACN; Flow rate: 60 mL/min; Gradient: 28% B to 58% B in 8 min, 58% B; Wavelength: 254 nm; 9.0 mg of 4-1-2 was obtained as a light brown solid. Combined the other batches, 26 mg of 4-1-2 was obtained. MS m/z [M+H]⁺ (ESI): 347.25. ¹H NMR (300 MHz, DMSO-d6) δ 8.06 (d, J=8.1 Hz, 1H), 7.75-7.67 (m, 2H), 7.44-7.32 (m, 2H), 7.02-6.97 (m, 1H), 3.40-3.35 (m, 2H), 3.12-3.07 (m, 2H), 2.84 (s, 6H), 2.66-2.56 (m, 1H), 1.65 (d, J=5.4 Hz, 3H), 1.11 (d, J=7.2 Hz, 6H).
26. Synthesis of 4-1-3 (Racemate)
In a similar manner described for 4-1-2, compound 4-1-3 was prepared from 300 mg of 4-1-1 and 306 mg of acetic acid. 5.0 mg of 4-1-3 was obtained as a light brown solid. Combined the other batches, 40 mg of 4-1-3 was obtained. MS m/z [M+H]⁺ (ESI): 319.10. ¹H NMR (400 MHz, DMSO-d6) δ10.16 (s, 1H), 8.08 (d, J=8.0 Hz, 1H), 7.75 (d, J=7.6 Hz, 1H), 7.65 (s, 1H), 7.43-7.32 (m, 2H), 7.01-6.97 (m, 1H), 3.39-3.33 (m, 2H), 3.15-3.11 (m, 2H), 2.83 (s, 6H), 2.10 (s, 3H), 1.63 (d, J=5.6 Hz, 3H).
27. Synthesis of 4-1-4 (racemate) In a similar manner described for 4-1-2, compound 4-1-4 was prepared from 300 mg of 4-1-1 and 520 mg of pivalic acid. The crude product (300 mg) was purified by Prep-HPLC with the following conditions (Column: XBridge Shield RP18 OBD Column, 30*150 mm, 5 m; Mobile Phase A: Water (10 mmol/L NH₄HCO_3+0.1% NH₃.H₂O), Mobile Phase B: ACN; Flow rate: 60 mL/min; Gradient: 48% B to 78% B in 9 min, 78% B; Wavelength: 254 nm. 5 mg of 4-1-4 was obtained as oil. MS m/z [M+H]⁺ (ESI): 361.25. ¹H NMR (300 MHz, DMSO-d6) δ 8.03 (d, J=8.1 Hz, 1H), 7.65-7.62 (m, 1H), 7.51 (s, 1H), 7.39-7.26 (m, 2H), 6.98-6.93 (m, 1H), 2.83-2.78 (m, 2H), 2.57-2.54 (m, 2H), 2.20 (s, 6H), 1.64 (d, J=5.4 Hz, 3H), 1.17 (s, 9H).
28. Synthesis of 5-1-4
To a stirred solution of 4-methoxy-1H-indole (1.0 g, 6.80 mmol, 1.0 equiv) in diethyl ether (20.0 mL) was added oxalyl chloride (0.9 g, 6.80 mmol, 1.0 equiv) dropwise at 0° C. under nitrogen atmosphere. The resulting mixture was stirred for additional 3 h at room temperature. The resulting mixture 5-1-1 was used in the next step directly without further purification.
To a stirred solution of dimethylamine hydrochloride (1.7 g, 21.04 mmol, 5.0 equiv) and triethylamine (3.4 g, 33.66 mmol, 8.0 equiv) in tetrahydrofuran (10.0 mL) was added 5-1-1 (crude, obtained at last step) dropwise at 0° C. under nitrogen atmosphere. The mixture was stirred for additional 0.5 h at room temperature. The reaction was quenched by the addition of water (50 mL) at 0° C. The resulting mixture was extracted with ethyl acetate (3×50 mL). The organic layer was dried over anhydrous sodium sulfate, filtered and concentrated under vacuum. The crude was purified by Flash with the following condition: Column, C18 silica gel; mobile layer, water (0.05% NH₄HCO₃) and acetonitrile (10% acetonitrile up to 100% in 15 min and hold 100% for 5 min); Detector, 254 nm. 600 mg (58% yield) of 5-1-2 was obtained as a yellow solid. MS m/z [M+H]⁺ (ESI): 247.10
To a stirred solution of 5-1-2 (600.0 mg, 2.44 mmol, 1.0 equiv) in tetrahydrofuran (10.0 mL) was added Lithium Aluminum Hydride (184.9 mg, 4.87 mmol, 2.0 equiv) in portions at 0° C. The resulting mixture was stirred for 1.5 days at 70° C. The reaction was quenched by the addition of water (20.0 mL) at 0° C. The aqueous layer was extracted with ethyl acetate (3×30 mL). The organic layer was dried over anhydrous sodium sulfate, filtered and concentrated under vacuum. The crude was purified by Flash with the following condition: Column, C18 silica gel; mobile layer, water (0.1% NH₃.H₂O+10 mmol/L NH₄HCO₃) and acetonitrile (10% acetonitrile up to 100% in 15 min and hold 100% for 5 min); Detector, 254 nm. 350 mg (66% yield) of 5-1-3 was obtained as a yellow solid. MS m/z [M+H]⁺ (ESI): 219.15.
To a stirred solution of 5-1-3 (80.0 mg, 0.37 mmol, 1.0 equiv) in dichloromethane (1.0 mL) was added tribromoborane (459.0 mg, 1.83 mmol, 5.0 equiv) dropwise at −78° C. under nitrogen atmosphere. The resulting mixture was stirred for 9 h at room temperature. The mixture was concentrated under vacuum. The residue was dissolved in dichloromethane (30.0 mL), added 50 mg of KHCO₃and 15.0 mL of methanol. After stirring for 30 min, the resulting mixture was concentrated under reduced pressure. The resulting mixture was dissolved in N,N-dimethylformamide (1.0 mL), added entanoic acid (30.0 mg, 0.15 mmol, 1.0 equiv), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (56.3 mg, 0.29 mmol, 2.0 equiv) and 4-dimethylaminopyridine (9.0 mg, 0.07 mmol, 0.5 equiv) at room temperature under nitrogen atmosphere. The resulting mixture was stirred for 16 h at room temperature. The reaction was diluted with ethyl acetate (3×10 mL), washed with water (3×10 mL). The organic phase was dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The crude product was purified by Flash with the following condition: Column, C18 silica gel; mobile layer, water (0.05% NH₄HCO₃) and acetonitrile (10% acetonitrile up to 100% in 20 min and hold 100% for 5 min); Detector, 254 nm. This resulted in 5-1-5 (20.0 mg, 47%) as a colorless oil. MS m/z [M+H]⁺ (ESI): 289.25. ¹H NMR (300 MHz, DMSO-d6) δ 11.03 (s, 1H), 7.23 (d, J=8.1 Hz, 1H), 7.15 (d, J=2.4 Hz, 1H), 7.06-7.01 (m, 1H), 6.66-6.63 (m, 1H), 2.78-2.65 (m, 4H), 2.47-2.43 (m, 2H), 2.20 (s, 6H), 1.73-1.63 (m, 2H), 1.48-1.35 (m, 2H), 0.94 (t, J=7.3 Hz, 3H).
Improved Prodrugs of Tryptamines, Such as N,N-dimethyltryptamine (DMT).
Prodrug 3-1-4, 4-1-2 and 4-1-4 was supposed to be stable in formulation and should release the parent compound in vivo.
Preliminary studies demonstrated the stability of prodrug (Tables 5-9). While solution stability has been shown for prodrug, the plasma stability data clearly showed release of parent drug in mouse plasma instantly. The following prodrugs were tested.
Prodrugs 3-1-4, 4-1-2 and 4-1-4 Stability Results in PBS pH 6.5 with and without Pepsin

TABLE 5

Detection of VLS-03-1 Parent in PBS pH 6.5, SGF with and without pepsin

Peak Area of MRM: 205.0/159.8

			VLS-03-1
Compound	Analyte	Incubation	standard	0 min	30 min	60 min	120 min	240 min

VLS-03-1	VLS-03-1 Parent	PBS (pH 6.5)	4.49E+04	5.04E+04	4.71E+04	4.71E+04	4.86E+04	4.75E+04
VLS-03-1	VLS-03-1 Parent	SGF with pepsin	4.22E+04	5.72E+04	5.37E+04	5.01E+04	5.48E+04	5.27E+04
VLS-03-1	VLS-03-1 Parent	SGF without	3.75E+04	4.53E+04	4.75E+04	4.51E+04	4.66E+04	4.57E+04
		pepsin

TABLE 6

Detection of VLS-03-10 Parent in PBS pH 6.5, SGF with and without pepsin

Peak Area of MRM: 188.7/144.0

			VLS-03-10
Compound	Analyte	Incubation	standard	0 min	30 min	60 min	120 min	240 min

VLS-03-10	VLS-03-10 Parent	PBS (pH 6.5)	4.34E+03	5.32E+03	4.68E+03	5.00E+03	4.72E+03	4.95E+03
VLS-03-10	VLS-03-10 Parent	SGF with pepsin	4.05E+03	5.13E+03	5.10E+03	5.25E+03	5.32E+03	5.28E+03
VLS-03-10	VLS-03-10 Parent	SGF without pepsin	3.46E+03	5.29E+03	4.98E+03	4.66E+03	4.93E+03	4.61E+03

TABLE 7

Detection of VLS-03-15 Parent in PBS pH 6.5, SGF with and without pepsin

Peak Area of MRM: 205.0/159.8

			VLS-03-15
Compound	Analyte	Incubation	standard	0 min	30 min	60 min	120 min	240 min

VLS-03-15	VLS-03-15 Parent	PBS (pH 6.5)	6.74E+05	6.56E+05	6.55E+05	6.28E+05	5.86E+05	5.51E+05
VLS-03-15	VLS-03-15 Parent	SGF with pepsin	6.26E+05	6.75E+05	7.21E+05	7.26E+05	7.14E+05	7.15E+05
VLS-03-15	VLS-03-15 Parent	SGF without pepsin	6.10E+05	7.05E+05	6.94E+05	6.55E+05	7.09E+05	6.96E+05

Data Summary

TABLE 8

Stability results of test compounds in PBS pH 6.5, SGF with and without pepsin

Remaining Percentage (%)

Compound	Incubation	T½ (min)	0 min	30 min	60 min	120 min	240 min

Chlorambucil	PBS (pH 6.5)	47.25	100.00	64.51	42.26	16.83	2.99
Erythromycin	SGF with pepsin	43.62	100.00	56.55	37.33	14.06	2.14
Erythromycin	SGF without pepsin	39.47	100.00	52.67	28.46	8.27	1.47
VLS-03-1	PBS (pH 6.5)	9999.00	100.00	96.32	99.71	100.03	101.35
VLS-03-1	SGF with pepsin	8950.90	100.00	101.74	90.49	102.16	96.47
VLS-03-1	SIG F without pepsin	9999.00	100.00	106.25	99.72	104.22	103.22
VLS-03-10	PBS (pH 6.5)	19876.96	100.00	92.62	100.03	92.63	97.66
VLS-03-10	SGF with pepsin	1604.11	100.00	96.82	93.09	91.42	89.32
VLS-03-10	SGF without pepsin	7008.36	100.00	99.86	104.95	98.41	98.86
VLS-03-15	PBS (pH 6.5)	922.46	100.00	96.93	89.82	89.44	82.54
VLS-03-15	SGF with pepsin	9999.00	100.00	97.29	107.53	103.18	102.22
VLS-03-15	SGF without pepsin	7839.52	100.00	101.12	103.35	105.28	97.98

If calculated T½ < 0, then T½ was reported as 9999 min.

Prodrugs 3-1-4, 4-1-2 and 4-1-4 Stability Results in Mouse Plasma

TABLE 9

Stability Results of Test Compounds in Mouse Plasma

Remaining Percentage (%)

Compound	Species	T½ (min)	0 min	15 min	30 min	60 min	120 min

Propantheline	Mouse	32.60	100.00	85.78	70.74	29.46	8.74
VLS-03-1		N.A.	100.00	0.00	0.00	0.00	0.00
VLS-03-10		N.A.	100.00	0.00	0.00	0.00	0.00
VLS-03-15		N.A.	100.00	0.00	0.00	0.00	0.00

N-Phosphonooxymethyl Prodrug 6-1-2 (PH-VLS-02-23-0)
Using methods described in Gunda I. George (Journal of Medicinal Chemistry, 1999, Vol. 42, 3095) N-Phosphonooxymethyl Prodrug compounds were prepared.
Illustration of the Prodrug Strategy:
6-1-2 was synthesized, as a novel prodrug strategy for improving the water solubility of tertiary amine-containing drugs. And tertiary amine DMT was converted in two step reaction to a very polar, N-Phosphonooxymethyl Prodrug 6-1-3.
The prodrug 6-1-2 releases the parent DMT in vivo through a two-step bio-reversion process. The rate determining first step is via an enzymatic process, where prodrug bio-reversion involves a phosphatase-catalyzed dephosphorylation to give the resultant hydroxymethyl quaternary ammonium intermediate and inorganic phosphate. The second step involves conversion of hydroxymethyl quaternary ammonium intermediate chemically to DMT and formaldehyde at physiological pH.
6-1-2 was synthesized, from commercially available in 3 simple steps.
Chemical stability of prodrug 6-1-2 (VLS-02-23) was evaluated and the results enumerated in Tables 10-11. PK studies of prodrug 6-1-2 and metabolite (VLS-02-23-10) in mice were also conducted and both plasma exposure and brain exposure were analyzed as shown FIGS. 1-2 , respectively.
Chemical Stability of N-Phosphonooxymethyl Prodrug 6-1-2 (VLS-02-23)

Data Summary

TABLE 10

Stability results of test compounds in PBS pH 6.5, SGF with and without pepsin

Remaininq Percentaqe (%)

Compound	Incubation	T½ (min)	0 min	30 min	60 min	120 min	240 min

Chlorambucil	PBS (pH 6.5)	43.17	100.00	61.12	43.17	15.12	2.15
Erythromycin	SGF with pepsin	40.26	100.00	56.55	34.88	11.22	1.60
Erythromycin	SGF without pepsin	37.36	100.00	59.45	33.60	10.27	1.20
VLS-02-23	PBS (pH 6.5)	9999.00	100.00	104.71	106.37	107.29	117.35
VLS-02-23	SGF with pepsin	6227.35	100.00	96.70	106.43	102.36	96.82
VLS-02-23	SIG F without pepsin	9999.00	100.00	98.76	107.08	101.90	108.80

Plasma Stability of N-Phosphonooxymethyl Prodrug 6-1-2 (VLS-02-23)

Data Summary

TABLE 11

Stability Results of Test Compounds in Mouse Plasma

Remaininq Percentage (%)

Compound	Species	T½ (min)	0 min	15 min	30 min	60 min	120 min

Propantheline	Mouse	34.09	100.00	80.81	64.04	34.86	9.05
VLS-02-03		568.34	100.00	114.24	110.21	109.95	91.16

Claims

What is claimed:

1. A compound of Formula (I):

or a pharmaceutically acceptable salt thereof;

wherein,

R₁and R₂are independently hydrogen, alkyl, cycloalkyl, or R₁and R₂together with the atoms to which they are attached form a heterocycle;

X, Y and Z are independently hydrogen, halogen, —O—, —S—, —NR_A—, or —(P═O)—OR_A(—OR′), wherein R_Ais hydrogen or alkyl;

R′, R₃, R₄, R₅and R₆are independently absent, hydrogen, alkyl, —(C═O)-alkyl, —(C═O)CHR₇, —(C═O)(CH₂)_n(NH₂), —(C)CH(NH₂)(CH₃), —(C═O)CH(NH₂), (CH₂)_n(NH)(C═NH), or —(C═O)(CH₂)_n(NH)(C═NH)—NH₂;

R₇and R₈are independently hydrogen or alkyl;

R₉is hydrogen, alkyl, or halogen;

L and M are independently alkyl or aryl;

W is oxygen, sulfur or NR₆;

n is an integer from 2-7; and

m is an integer from 1-4,

wherein at least one of R′, R₃, R₄, and R₅and R₆is —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or

2. The compound of claim 1, wherein R₁and R₂are independently C₁-C₆alkyl.

3. The compound of claim 1 or 2, wherein R₁and R₂are independently methyl, ethyl, propyl, and isopropyl.

4. The compound of any one of claims 1-3, wherein R₁and R₂are methyl.

5. The compound of any one of claims 1-4, wherein X, Y and Z are H.

6. The compound of any one of claims 1-5, wherein R₈is hydrogen.

7. The compound of any one of claims 1-6, wherein R₈is alkyl.

8. The compound of any one of claims 1-7, wherein R₈is C₁-C₆alkyl.

9. The compound of any one of claims 1-8, wherein R₉is a halogen.

10. The compound of any one of claims 1-9, wherein R₉is Br.

11. The compound of any one of claims 1-8, wherein R₉is alkyl.

12. The compound of any one of claims 1-8 or 11, wherein R₉is C₁-C₆alkyl.

13. The compound of any one of claims 1-8, wherein R₇and R₈are independently hydrogen or C₁-C₈alkyl and R₉is hydrogen, halogen, or C₁-C₈alkyl.

14. The compound of claim 13, wherein the halogen is bromine.

15. The compound of claim 13, wherein said C₁-C₈alkyl is substituted with at least one of hydroxyl, thiol and halogen.

16. The compound of any one of claims 1-15, wherein m is 1.

17. The compound of any one of claims 1-15, wherein m is 2.

18. The compound of claim 1, wherein X, Y and Z are hydrogen; W is NR₆; and R₆is —(C═O)— alkyl or —(C═O)(CH₂)_n(NH₂).

19. The compound of claim 1, wherein two of X, Y and Z are hydrogen.

20. The compound of claim 19, wherein the non-hydrogen X, Y or Z is —O—, —S—, —NR_A—, or —(P═O)—OR_A(—OR′), wherein R_Ais hydrogen or alkyl.

21. The compound of claim 1, wherein X and Y are hydrogen; Z is —O—, —S—, —NR_A—, or —(P═O)—OR_A(—OR′), wherein R_Ais hydrogen or alkyl.

22. The compound of claim 1, wherein X and Z are hydrogen; Y is —O—, —S—, —NR_A—, or —(P═O)—OR_A(—OR′), wherein R_Ais hydrogen or alkyl.

23. The compound of claim 1, wherein Y and Z are hydrogen; X is —O—, —S—, —NR_A—, or —(P═O)—OR_A(—OR′), wherein R_Ais hydrogen or alkyl.

24. The compound of any one of claims 1-23, wherein R₅is —(C═O)-alkyl or —(C═O)(CH₂)_n(NH₂).

25. The compound of any one of claims 1-24, wherein R₄is —(C═O)-alkyl or —(C═O)(CH₂)_n(NH₂).

26. The compound of any one of claims 1-25, wherein R₃is —(C═O)-alkyl or —(C═O)(CH₂)_n(NH₂).

27. The compound of any one of claims 1-26, wherein R₆is —(C═O)-alkyl or —(C═O)(CH₂)_n(NH₂).

28. The compound of any one of claims 1-23, wherein at least one of R′, R₃, R₄, R₅and R₆is —(C═O)(CH₂)_n(NH₂) and n is 3.

29. The compound of any one of claims 1-23, wherein at least one of R′, R₃, R₄, R₅and R₆is —(C═O)CH(NH₂)(CH₂)_n(NH₂), and n is 4.

30. The compound of any one of claims 1-23, wherein at least one of R′, R₃, R₄, R₅and R₆is —(C═O)(CH₂)_n(NH)(C═NH)—NH₂and n is 3.

31. The compound of any one of claims 1-23, wherein at least one of R′, R₃, R₄, R₅and R₆is —(C═O)CH(NH)(CH₂)_n(C═NH)—NH₂and n is 2.

32. The compound of any one of claims 1-31, wherein L is methyl or isopropyl and M is alkyl or aryl.

33. The compound of any one of claims 1-32, wherein W is NR₆and R₆is hydrogen or alkyl.

34. A compound of claim 1, which is a salt of the Formula (IV):

wherein R₁₀is —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or

A is a pharmaceutically acceptable anion.

35. The compound of any one of claims 1-34, wherein the compound is a compound shown in Table 1, Table 2, Table 3, or Table 4.

36. A compound of Formula (III):

or a pharmaceutically acceptable salt thereof;

wherein,

X, Y and Z are independently hydrogen, halogen, —O—, —S—, —NR_A—, or —(P═O)—OR_A(—OR′), wherein R_Ais hydrogen or alkyl,

R′, R₃, R₄, and R₅are independently absent, hydrogen, alkyl, —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)CH(NH₂) (CH₂)_n(NH)(C═NH)—NH₂, —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or

R₇and R₈are independently hydrogen or alkyl;

R₉is hydrogen, alkyl or halogen;

L and M are independently alkyl or aryl;

n is an integer from 2-7; and

m is an integer from 1-4,

wherein at least one of R′, R₃, R₄, and R₅is —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or

37. The compound of claim 36, wherein R₁and R₂are independently C₁-C₆alkyl.

38. The compound of claim 36 or 37, wherein R₁and R₂are independently methyl, ethyl, propyl, and isopropyl.

39. The compound of any one of claims 36-38, wherein R₁and R₂are methyl.

40. The compound of any one of claims 36-39, wherein X, Y and Z are H.

41. The compound of any one of claims 36-40, wherein R₈is hydrogen.

42. The compound of any one of claims 36-41, wherein R₈is alkyl.

43. The compound of any one of claims 36-42, wherein R₈is C₁-C₆alkyl.

44. The compound of any one of claims 36-43, wherein R₉is hydrogen.

45. The compound of any one of claims 36-43, wherein R₉is alkyl.

46. The compound of claim 45, R₉is C₁-C₆alkyl.

47. The compound of any one of claims 36-46, wherein R₇and R₈are independently hydrogen or C₁-C₈alkyl and R₉is hydrogen, halogen or C₁-C₈alkyl.

48. The compound of claim 47, wherein the C₁-C₈alkyl is substituted with at 1-3 groups including hydroxyl, thiol or halogen.

49. The compound of any one of claims 36-48, wherein m is 1.

50. The compound of any one of claims 36-48, wherein m is 2.

51. The compound of any one of claims 1-39 or 41-50, wherein two of X, Y and Z are hydrogen.

52. The compound of claim 51, wherein the non-hydrogen X, Y or Z is —O—, —S—, —NR_A—, or —(P═O)—OR_A(—OR′), wherein R_Ais hydrogen or alkyl.

53. The compound of claim 36, wherein X and Y are hydrogen; Z is —O—, —S—, —NR_A—, or —(P═O)—OR_A(—OR′), wherein R_Ais hydrogen or alkyl.

54. The compound of claim 36, wherein X and Z are hydrogen; Y is —O—, —S—, —NR_A—, or —(P═O)—OR_A(—OR′), wherein R_Ais hydrogen or alkyl.

55. The compound of claim 36, wherein Y and Z are hydrogen; X is —O—, —S—, —NR_A—, or —(P═O)—OR_A(—OR′), wherein R_Ais hydrogen or alkyl.

56. The compound of any one of claims 36-55, wherein R₄is —(C═O)-alkyl, or —(C═O)(CH₂)_n(NH₂).

57. The compound of any one of claims 36-56, wherein R₅is —(C═O)-alkyl or —(C═O)(CH₂)_n(NH₂).

58. The compound of any one of claims 36-57, wherein R₃is —(C═O)-alkyl, or —(C═O)(CH₂)_n(NH₂).

59. The compound of any one of claims 36-55, wherein at least one of R′, R₃, R₄, and R₅is —(C═O)(CH₂)_n(NH₂) and n is 3.

60. The compound of any one of claims 36-55, wherein at least one of R′, R₃, R₄, and R₅is —(C═O)CH(NH₂)(CH₂)_n(NH₂) and n is 4.

61. The compound of any one of claims 36-55, wherein at least one of R′, R₃, R₄, and R₅is —(C═O)(CH₂)_n(NH)(C═NH)—NH₂and n is 3.

62. The compound of any one of claims 36-55, wherein at least one of R′, R₃, R₄, and R₅is —(C═O)CH(NH₂)(CH₂)_n(NH)(C═NH)—NH₂and n is 2.

63. The compound of any one of claims 36-55, wherein at least one of R′, R₃, R₄, and R₅is

64. The compound of any one of claims 36-63, in salt form, of the Formula (V):

wherein R₁₀is —(C═O)-alkyl, —(C═O)CHR₇(NH₂), —(C═O)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH₂), —(C═O)CH(NH₂)(CH₂)_n(NH)(C═NH)—NH₂, —(C═O)(CH₂)_n(NH)(C═NH)—NH₂or

and

wherein A is a pharmaceutically acceptable anion.

65. The compound claim 64, wherein L is methyl or isopropyl and M is alkyl or aryl.

66. A pharmaceutical composition comprising the compound of any one of claims 1-65 and a pharmaceutically acceptable excipient.

67. A compound as shown in Table 2, wherein X and Y are hydrogen and R₃and R₄are absent.

68. A compound as shown in Table 2, wherein X is hydrogen, R₃is absent, Y is O, and R₄is hydrogen.

69. A compound as shown in Table 2, wherein X is hydrogen, R₃is absent, Y is O, and R₄is Me.

70. A compound as shown in Table 2, wherein X is hydrogen, R₃is absent, Y is O, and R₄is Ac.

71. A compound as shown in Table 2, wherein X is O, R₃is hydrogen, Y is hydrogen, and R₄is absent.

72. A compound as shown in Table 2, wherein X is O, R₃is Me, Y is hydrogen, and R₄is absent.

73. A compound as shown in Table 2, wherein X is O, R₃is Ac, Y is hydrogen, and R₄is absent.

74. A compound of the formula:

75. A compound of the formula:

76. A compound of the formula

77. A compound of the formula:

78. A compound of Formula (VI):

R₃, R₄, R₅and R₆are independently absent, hydrogen, alkyl, —(C═O)-alkyl, —(C═O)CHR₇, —(C═O)(CH₂)_n(NH₂), —(C)CH(NH₂)(CH₃), —(C═O)CH(NH₂), (CH₂)_n(NH)(C═NH), or —(C═O)(CH₂)_n(NH)(C═NH)—NH₂;

X is carbon or nitrogen;

W is carbon, oxygen, or nitrogen; and

Z is hydrogen, deuterium, or oxygen.

79. The compound of claim 78, having the following chemical formula:

80. A compound selected from the formulae:

81. A phosphonooxymethyl prodrug according to claim 1 of the Formula (VIII):

wherein

Ru and R₁₂are pharmaceutically acceptable salt and are independently H, D, Na⁺, K⁺, NH₄ ⁺; or

R₁and R₁₂are independently a pharmaceutically acceptable salt, hydrogen, deuterium, alkyl, substituted alkyl, cycloalkyl, or R₁₁and R₁₂together with the atoms to which they are attached form an alkyl, cycloalkyl, heterocycles and are ether, hemiaminal ketal and amino acid, wherein the pharmaceutically acceptable salt is Na⁺, K⁺, NH₄ ⁺.

82. A pharmaceutical composition comprising a compound of any one of claims 1-65 or 67-81 and a pharmaceutically acceptable excipient.

83. An oral dosage form comprising the pharmaceutical composition of claim 66 or 82.

84. A method of treating post-traumatic stress disorder (PTSD) in a subject in need thereof, comprising administering a therapeutically effective amount of a compound of any one of claims 1-65 or 67-81 or a pharmaceutical composition of claims 66, 82 or 83 to the subject.

85. A method of treating depression in a subject in need thereof, comprising administering a therapeutically effective amount of a compound of any one of claims 1-65 or 67-81 or a pharmaceutical composition of claims 66, 82 or 83 to the subject.

86. The method of claim 85, wherein the depression is major depressive disorder (MDD) or treatment-resistant depression (TRD).

87. A method of treating an anxiety disorder in a subject in need thereof, comprising administering a therapeutically effective amount of a compound of any one of claims 1-65 or 67-81 or a pharmaceutical composition of claims 66, 82 or 83 to the subject.