US20220409058A1 - Handheld probe and system for imaging human tissue - Google Patents
Handheld probe and system for imaging human tissue Download PDFInfo
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- US20220409058A1 US20220409058A1 US17/872,299 US202217872299A US2022409058A1 US 20220409058 A1 US20220409058 A1 US 20220409058A1 US 202217872299 A US202217872299 A US 202217872299A US 2022409058 A1 US2022409058 A1 US 2022409058A1
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Definitions
- This invention relates to diffusion optical spectroscopy and, in particular, to a handheld probe and system using the handheld probe for cross-sectional imaging of human tissue.
- breast cancer is the most common cancer in women and it is ranked as the second cause of death, in North America.
- Breast cancer affects approximately one in eight North America women during their lifetime. Treatment entails a number of steps from the time of diagnoses to a consistent follow-up after the treatment stage. These steps have to be taken seriously if there is any suspicions lesion.
- Introducing new technology or improving one or more of the assessment parameters including: accuracy, sensitivity, specificity or positive predictive value on current techniques may help physicians to detect breast cancer in its early stage, which is crucial in women's health.
- the screening methods of clinical breast exam (CBE) and X-ray mammography are questionable for their lower sensitivity.
- X-ray mammography is a preliminary screening methodology, it is painful and uses harmful ionizing X-ray radiation.
- Ultrasound, x-ray mammography, and magnetic resonance imaging (MRI) are commonly used for breast cancer detection where X-ray mammography is the primary screening technique for breast cancer.
- X-ray mammography is the principle modality for breast cancer screening, it is recommended for women over the age of 50, because it has low sensitivity (67.8%) for younger women or women with dense breasts. In addition, it has a potential health risk as an effect of ionizing radiations. Therefore, because of X-ray mammography's limitations, researchers have been encouraged to use different breast cancer detection modalities, such as diffuse optical tomography, impedance spectroscopy and elasto-graphy.
- NIR optical imaging in association with optoelectronic devices and fiber optics, also have been developed very rapidly and currently NIR optical imaging approaches have been advanced for breast cancer diagnosis. This technique is known as a noninvasive and promising method for compositional and functional imaging of extremely scattering mediums, such as brain and breast.
- the optical properties of the breast tissue have been widely studied. For example, it is known that the absorption of the four main constituents of breast tissue, namely water, fat, deoxyhemoglobin (Hb) and oxyhemoglobin (HbO 2 ), is low in the wavelength spectrum from 650 nm to 1100 nm, which allows light to travel deeply into the breast tissue.
- Hb deoxyhemoglobin
- HbO 2 oxyhemoglobin
- a number of handheld diffuse optical probes have been implemented and tested for breast cancer detection and screening. Almost all of the probes are designed in reflectance geometry to measure optical properties of the breast tissue underneath of the probes. Laser light sources or fiber coupled laser light sources in near infrared range are used to determine the concentration of main chromophores in the breast tissue related to sensing anomalies.
- laser and fiber-optic based illumination techniques are complex, large in size and costly.
- An object of the invention is to address the above shortcomings.
- a diffuse-optical-spectroscopy system for scanning human tissue.
- the system includes: (a) a handheld probe operable to emit electromagnetic radiation at one or more wavelengths corresponding to absorption associated with one or more human-tissue constituents, respectively, the handheld probe being operable to detect received electromagnetic radiation at each of the one or more wavelengths; and (b) a processor operable to produce, in response to the received electromagnetic radiation, one or more cross-sectional images of the human tissue respectively associated with the one or more wavelengths.
- the handheld probe may include one or more sensors for detecting the received electromagnetic radiation, the one or more sensors being aligned along a first axis and facing in an outward direction.
- the handheld probe may include first and second sources for emitting the electromagnetic radiation, the first and second sources being aligned along the first axis, facing in the outward direction, and disposed on either side of the one or more sensors.
- the one or more wavelengths may include at least four wavelengths corresponding to absorption associated with at least four human-tissue constituents. Each of the at least four wavelengths may be in at least one of a visible region, a near-infrared region, and an infrared region of the electromagnetic spectrum.
- the at least four human-tissue constituents may include deoxyhemoglobin, oxyhemoglobin, water and fat.
- Each of the first and second sources may include first, second, third and fourth light sources operable to emit the electromagnetic radiation at first, second, third and fourth wavelengths, respectively, each of the first, second, third and fourth light sources being selected from the group consisting of a light-emitting diode, an encapsulated light-emitting diode, and a laser.
- the handheld probe may include a linear array detector (charge-coupled device or linear photodiode) that includes the one or more sensors:
- the electromagnetic radiation on may be continuous wave electromagnetic radiation.
- a method of scanning human tissue by diffuse-optical-spectroscopy involves: (a) emitting electromagnetic radiation at one or more wavelengths corresponding to absorption associated with one or more human-tissue constituents, respectively, by a handheld probe placed in proximity to the human tissue: (b) detecting by the handheld probe received electromagnetic radiation at each of the one or more wavelengths; and (c) in response to the received electromagnetic radiation, producing by a processor one or more cross-sectional images of the human tissue respectively associated with the one or more wavelengths.
- Step (b) of the method may involve detecting the received electromagnetic radiation by one or more sensors of the handheld probe when the one or more sensors are aligned along a first axis and facing in an outward direction.
- Step (a) of the method may involve emitting the electromagnetic radiation by first and second sources of the handheld probe when the first and second sources are aligned along the first axis, facing in the outward direction, and disposed on either side of the one or more sensors.
- Step (a) of the method may involve emitting the electromagnetic radiation at the one or more wavelengths that may include at least four wavelengths corresponding to absorption associated with at least four human-tissue constituents, each of the at least four wavelengths being in at least one of a visible region, a near-infrared region, and an infrared region of the electromagnetic spectrum, the at least four human-tissue constituents comprising deoxyhemoglobin, oxyhemoglobin, water and fat.
- Step (a) of the method may involve emitting the electromagnetic radiation by first, second, third and fourth light sources at first, second, third and fourth wavelengths, respectively when each of the first, second., third and fourth light sources are selected from the group consisting of a light-emitting diode, an encapsulated light-emitting diode, and a laser.
- Step (b) of the method may involve detecting the received electromagnetic radiation by at least one of a charge-coupled device and an array photodiode of the handheld probe when the at least one of a charge-coupled device and an array photodiode comprises the one or more sensors.
- Step (a) of the method may involve emitting continuous wave electromagnetic radiation.
- a system for scanning human tissue by diffuse-optical-spectroscopy includes: (a) source means for emitting electromagnetic radiation at one or more wavelengths corresponding to absorption associated with one or more human-tissue constituents, respectively; (b) receiving means for detecting received electromagnetic radiation at each of the one or more wavelengths; and (c) processing means for producing, in response to the received electromagnetic radiation, one or more cross-sectional images of the human tissue respectively associated with the one or more wavelengths.
- DOS Diffuse optical spectroscopy
- DCI diffuse optical Imaging
- the DOB-Scan probe is used to measure optical properties of breast tissue and create functional and compositional cross-sectional images of the breast.
- a linear charge coupled device (CCD) measures the intensity of the scattered photons at different radial destinations from the illumination source on the surface of the breast tissue.
- the proposed method replaces fiber optic based illumination techniques, which increases the complexity, size and cost of a potential probe; by multi-wavelengths eLED which acts as a pencil beam source in such a scattering media like the breast tissue.
- the DOB-Scan probe and associated algorithm can create cross-sectional images of the breast tissue underneath of the probe by using high resolution linear CCD.
- the introduced technique miniaturizes the probe, while maintaining reliability and accuracy of this technique in breast imaging.
- Multi-wavelength light sources are used to increase the capability of the probe by creating separate images for four primary chromophores of the breast tissue including deoxyhemoglobin, oxyhemoglobin, fat and water.
- the average scattering coefficient of the medium and localized concentration variations in oxyhemoglobin and deoxyhemoglobin can be measured utilizing the probe.
- DOB-Scan probe In order to evaluate the performance of DOB-Scan probe, a series of tissue-like materials (phantoms), containing of Intralipid®, Black ink, Defrin®, and PierceTM have been used to produce the experimental results indicated below. The test results provide evidence that the DOB-Scan probe can detect any inhomogeneity inside the phantoms.
- FIG. 1 is a schematic representation of a handheld probe according to a first embodiment of the invention, showing sources and a detector;
- FIG. 2 is a photograph of the prototypical handheld probe shown in FIG. 1 , showing locations of first and second eLEDs and a linear CCD;
- FIG. 3 is a photograph of a prototypical system according to the first embodiment, showing the prototypical handheld probe connected to a host computer;
- FIG. 4 is a schematic representation of the system shown in FIG. 3 , showing a wireless communication unit
- FIG. 5 is a schematic representation of the handheld probe shown in FIG. 1 , showing a highly scattering medium
- FIG. 6 is a photograph of a breast phantom for use in testing the prototypical system shown in FIG. 3 , showing a hole in the breast phantom for containing liquid providing a phantom representation of a cancerous tumor:
- FIG. 7 a is a photograph of the breast phantom of FIG. 6 and the prototypical system of FIG. 3 , showing the prototypical handheld probe's middle placed along the center of the hole of the breast phantom containing India black ink;
- FIG. 7 b is a photograph of the breast phantom of FIG. 6 and the prototypical system of FIG. 3 , showing the prototypical handheld probe's middle placed to the left of the hole of the breast phantom containing India black ink;
- FIG. 7 c is a photograph of the breast phantom of FIG. 6 and the prototypical system of FIG. 3 , showing the prototypical handheld probe's middle placed to the right of the hole of the breast phantom containing India black ink;
- FIG. 8 a is a cross-sectional image produced by the prototypical system shown in FIG. 3 when the probe is emitting electromagnetic radiation at 690 nm and is located along the center of the breast phantom's hole containing India black ink;
- FIG. 8 b is a cross-sectional image produced by the prototypical system shown in FIG. 3 when the probe is emitting electromagnetic radiation at 690 nm and is located to the left of the breast phantom's hole containing India black ink;
- FIG. 8 c is a cross-sectional image produced by the prototypical system shown in FIG. 3 when the probe is emitting electromagnetic radiation at 690 nm and is located to the right of the breast phantom's hole containing India black ink;
- FIG. 9 a is a cross-sectional image produced by the prototypical system shown in FIG. 3 when the probe is emitting electromagnetic radiation at 690 nm and is located along the center of the breast phantom's hole containing a protein assay reagent;
- FIG. 9 b is a cross-sectional image produced by the prototypical system shown in FIG. 3 when the probe is emitting electromagnetic radiation at 690 nm and is located to the left of the breast phantom's hole containing the protein assay reagent;
- FIG. 9 c is a cross-sectional image produced by the prototypical system shown in FIG. 3 when the probe is emitting electromagnetic radiation at 690 nm and is located to the right of the breast phantom's hole containing the protein assay reagent;
- FIG. 10 a is a cross-sectional image produced by the prototypical system shown in FIG. 3 when the probe is emitting electromagnetic radiation at 800 nm and is located along the center of the breast phantom's hole containing the protein assay reagent;
- FIG. 10 b is a cross-sectional image produced by the prototypical system shown in FIG. 3 when the probe is emitting electromagnetic radiation at 800 nm and is located to the left of the breast phantom's hole containing the protein assay reagent:
- FIG. 10 c is a cross-sectional image produced by the prototypical system shown in FIG. 3 when the probe is emitting electromagnetic radiation at 800 nm and is located to the right of the breast phantom's hole containing the protein assay reagent;
- FIG. 11 a is a cross-sectional image produced by the prototypical system shown in FIG. 3 , showing reddish areas or lack thereof corresponding to the location of the breast phantom's hole containing a reference solution with India black ink in the concentration of 0.125 ml/l when the prototypical handheld probe is emitting electromagnetic radiation at 690 nm;
- FIG. 11 b is a cross-sectional image produced by the prototypical system shown in FIG. 3 , showing reddish areas or lack thereof corresponding to the location of the breast phantom's hole containing the reference solution with India black ink in the concentration of 0.25 ml/l when the prototypical handheld probe is emitting electromagnetic radiation at 690 nm;
- FIG. 11 c is a cross-sectional image produced by the prototypical system shown in FIG. 3 , showing reddish areas or lack thereof corresponding to the location of the breast phantom's hole containing the reference solution with India black ink in the concentration of 0.375 ml/l when the prototypical handheld probe is emitting electromagnetic radiation at 690 nm;
- FIG. 11 d is a cross-sectional image produced by the prototypical system shown in FIG. 3 , showing reddish areas or lack thereof corresponding to the location of the breast phantom's hole containing the reference solution with India black ink in the concentration of 0.5 ml/l when the prototypical handheld probe is emitting electromagnetic radiation at 690 nm;
- FIG. 11 e is a cross-sectional image produced by the prototypical system shown in FIG. 3 , showing reddish areas or lack thereof corresponding to the location of the breast phantom's hole containing the reference solution with India black ink in the concentration of 0.625 ml/I when the prototypical handheld probe is emitting electromagnetic radiation at 690 nm;
- FIG. 11 f is a cross-sectional image produced by the prototypical system shown in FIG. 3 , showing reddish areas or lack thereof corresponding to the location of the breast phantom's hole containing the reference solution with India black ink in the concentration of 0.75 ml/l when the prototypical handheld probe is emitting electromagnetic radiation at 690 nm;
- FIG. 12 a is a sectional image associated with patient case 1, a wavelength of 690 nm, and the left breast of patient 1 ;
- FIG. 12 b is a sectional image associated with patient case 1, a wavelength of 690 nm, and the right breast of patient 1 ;
- FIG. 12 c is a sectional image associated with patient case 1, a wavelength of 750 nm, and the left breast of patient 1 ;
- FIG. 12 d is a sectional image associated with patient case 1, a wavelength of 750 nm, and the right breast of patient 1 ;
- FIG. 12 e is a sectional image associated with patient case 1, a wavelength of 800 nm, and the left breast of patient 1 ;
- FIG. 12 f is a sectional image associated with patient case 1, a wavelength of 800 nm, and the right breast of patient 1 ;
- FIG. 12 g is a sectional image associated with patient case 1, a wavelength of 850 nm, and the left breast of patient 1 ;
- FIG. 12 h is a sectional image associated with patient case 1, a wavelength of 850 nm, and the right breast of patient 1 ;
- FIG. 13 a is a sectional image associated with patient case 2, a wavelength of 690 nm, and the left breast of patient 2 ;
- FIG. 13 b is a sectional image associated with patient case 2, a wavelength of 690 nm, and the right breast of patient 2 ;
- FIG. 13 c is a sectional image associated with patient case 2, a wavelength of 750 nm, and the left breast of patient 2 ;
- FIG. 13 d is a sectional image associated with patient case 2, a wavelength of 750 nm, and the right breast of patient 2 ;
- FIG. 13 e is a sectional image associated with patient case 2, a wavelength of 800 nm, and the left breast of patient 2 ;
- FIG. 13 f is a sectional image associated with patient case 2, a wavelength of 800 nm, and the right breast of patient 2 ;
- FIG. 13 g is a sectional image associated with patient case 2, a wavelength of 850 nm, and the left breast of patient 2 ;
- FIG. 13 h is a sectional image associated with patient case 2, a wavelength of 850 nm, and the right breast of patient 2 ;
- FIG. 14 a is a sectional image associated with patient case 3, a wavelength of 690 nm, and the left breast of patient 3 ;
- FIG. 14 b is a sectional image associated with patient case 3, a wavelength of 690 nm, and the right breast of patient 3 ;
- FIG. 14 c is a sectional image associated with patient case 3, a wavelength of 750 nm, and the left breast of patient 3 ;
- FIG. 14 d is a sectional image associated with patient case 3, a wavelength of 750 nm, and the right breast of patient 3 ;
- FIG. 14 e is a sectional image associated with patient case 3, a wavelength of 800 nm, and the left breast of patient 3 ;
- FIG. 14 f is a sectional image associated with patient case 3, a wavelength of 800 nm, and the right breast of patient 3 ;
- FIG. 14 g is a sectional image associated with patient case 3, a wavelength of 850 nm, and the left breast of patient 3 ;
- FIG. 14 h is a sectional image associated with patient case 3, a wavelength of 850 nm, and the right breast of patient 3 ;
- FIG. 15 a is a sectional image associated with patient case 4, a wavelength of 690 nm, and the left breast of patient 4 ;
- FIG. 15 b is a sectional image associated with patient case 4, a wavelength of 690 nm, and the right breast of patient 4 ;
- FIG. 15 c is a sectional image associated with patient case 4, a wavelength of 750 nm, and the left breast of patient 4 ;
- FIG. 15 d is a sectional image associated with patient case 4, a wavelength of 750 nm, and the right breast of patient 4 ;
- FIG. 15 e is a sectional image associated with patient case 4, a wavelength of 800 nm, and the left breast of patient 4 ;
- FIG. 15 f is a sectional image associated with patient case 4, a wavelength of 800 nm, and the right breast of patient 4 ;
- FIG. 15 g is a sectional image associated with patient case 4, a wavelength of 850 nm, and the left breast of patient 4 ;
- FIG. 15 h is a sectional image associated with patient case 4, a wavelength of 850 nm, and the right breast of patient 4 ;
- Figure a is a sectional image associated with patient case 5, a wavelength of 690 nm, and the left breast of patient 5 ;
- FIG. 6 b is a sectional image associated with patient case 5, a wavelength of 690 nm, and the right breast of patient 5 ;
- FIG. 16 c is a sectional image associated with patient case 5, a wavelength of 750 nm, and the left breast of patient 5 ;
- FIG. 16 d is a sectional image associated with patient case 5, a wavelength of 750 nm, and the right breast of patient 5 ;
- FIG. 16 e is a sectional image associated with patient case 5, a wavelength of 800 nm, and the left breast of patient 5 ;
- FIG. 16 f is a sectional image associated with patient case 5, a wavelength of 800 nm, and the right breast of patient 5 ;
- FIG. 16 g is a sectional image associated with patient case 5, a wavelength of 850 nm, and the left breast of patient 5 ;
- FIG. 16 h is a sectional image associated with patient case 5, a wavelength of 850 nm, and the right breast of patient 5 ;
- FIG. 17 a is a sectional image associated with patient case 6, a wavelength of 690 nm, and the left breast of patient 6 ;
- FIG. 17 b is a sectional image associated with patient case 6, a wavelength of 690 nm, and the right breast of patient 6 ;
- FIG. 17 c is a sectional image associated with patient case 6, a wavelength of 750 nm, and the left breast of patient 6 ;
- FIG. 17 d is a sectional image associated with patient case 6, a wavelength of 750 nm, and the right breast of patient 6 ;
- FIG. 17 e is a sectional image associated with patient case 6, a wavelength of 800 nm, and the left breast of patient 6 ;
- FIG. 17 f is a sectional image associated with patient case 6, a wavelength of 800 nm, and the right breast of patient 6 ;
- FIG. 17 g is a sectional image associated with patient case 6, a wavelength of 850 nm, and the left breast of patient 6 ;
- FIG. 17 h is a sectional image associated with patient case 6, a wavelength of 850 nm, and the right breast of patient 6 ;
- Figure a is a sectional image associated with patient case 7, a wavelength of 690 nm, and the left breast of patient 7 ;
- FIG. 8 b is a sectional image associated with patient case 7, a wavelength of 690 nm, and the right breast of patient 7 ;
- FIG. 18 c is a sectional image associated with patient case 7, a wavelength of 750 nm, and the left breast of patient 7 ;
- FIG. 18 d is a sectional image associated with patient case 7, a wavelength of 750 nm, and the right breast of patient 7 ;
- FIG. 18 e is a sectional image associated with patient case 7, a wavelength of 800 nm, and the left breast of patient 7 ;
- FIG. 18 f is a sectional image associated with patient case 7, a wavelength of 800 nm, and the right breast of patient 7 ;
- FIG. 18 g is a sectional image associated with patient case 7, a wavelength of 850 nm, and the left breast of patient 7 ;
- FIG. 18 h is a sectional image associated with patient case 7, a wavelength of 850 nm, and the right breast of patient 7 ;
- FIG. 19 a is a sectional image associated with patient case 9, a wavelength of 690 nm, and the left breast of patient 9 ;
- FIG. 19 b is a sectional image associated with patient case 9, a wavelength of 690 nm, and the right breast of patient 9 ;
- FIG. 19 c is a sectional image associated with patient case 9, a wavelength of 750 nm, and the left breast of patient 9 ;
- FIG. 19 d is a sectional image associated with patient case 9, a wavelength of 750 nm, and the right breast of patient 9 ;
- FIG. 19 e is a sectional image associated with patient case 9, a wavelength of 800 nm, and the left breast of patient 9 ;
- FIG. 19 f is a sectional image associated with patient case 9, a wavelength of 800 nm, and the right breast of patient 9 ;
- FIG. 19 g is a sectional image associated with patient case 9, a wavelength of 850 nm, and the left breast of patient 9 ;
- FIG. 19 h is a sectional image associated with patient case 9, a wavelength of 850 nm, and the right breast of patient 9 ;
- FIG. 20 a is a sectional image associated with patient case 10, a wavelength of 690 nm, and the left breast of patient 10 ;
- FIG. 20 b is a sectional image associated with patient case 10, a wavelength of 690 nm, and the right breast of patient 10 ;
- FIG. 20 c is a sectional image associated with patient case 10, a wavelength of 750 nm, and the left breast of patient 10 ;
- FIG. 20 d is a sectional image associated with patient case 10, a wavelength of 750 nm, and the right breast of patient 1
- FIG. 20 e is a sectional image associated with patient case 10, a wavelength of 800 nm, and the left breast of patient 10 ;
- FIG. 20 f is a sectional image associated with patient case 10, a wavelength of 800 nm, and the right breast of patient 10 ;
- FIG. 20 g is a sectional image associated with patient case 10, a wavelength of 850 nm, and the left breast of patient 10 ;
- FIG. 20 h is a sectional image associated with patient case 10, a wavelength of 850 nm, and the right breast of patient 10 .
- a system for scanning human tissue by diffuse-optical-spectroscopy includes: (a) source means for emitting electromagnetic radiation at one or more wavelengths corresponding to absorption associated with one or more human-tissue constituents, respectively; (b) receiving means for detecting received electromagnetic radiation at each of the one or more wavelengths; and (c) processing means for producing, in response to the received electromagnetic radiation, one or more cross-sectional images of the human tissue respectively associated with the one or more wavelengths.
- a handheld diffuse optical breast scanning probe an exemplary prototype of which is variously referred to herein as the probe and/or the DOB-Scan probe 10 , is operable for functional and compositional cross-sectional imaging of breast tissue 12 ( FIG. 5 ).
- the DOB-Scan probe 10 is tested on breast tissue-mimicking phantoms 14 ( FIGS. 6 , 7 a , 7 b and 7 c ) which have similar optical properties of breast tissue 12 and, thereafter, tested on human subjects.
- the DOB-Scan probe uses single-wavelength or multi-wavelengths light emitting diode(s) (LED), encapsulated light emitting diode(s) (eLED) 16 , and/or laser(s) as an illumination source containing one or more visible, near infrared, and/or infrared wavelengths such as four near infrared wavelengths, 690 nm, 750 nm, 800 nm, and 850 nm, in one embodiment, LEDs of each eLED 16 are set 0.5 mm apart from each other (within the eLED 16 ) and perform as a multi-wavelengths pointed illumination source in such a scattering medium, A linear charge coupled device (CCD) 18 , linear array photodiode(s), and/or a photodiode-array detector of the DOB-Scan probe 10 is used to measure the intensity of the scattered photons, in a line with 2048 points, on the surface of the breast 12 (or phantom 14 )
- the DOB-Scan probe 10 is used to create two-dimensional cross-sectional images of breast tissue 12 in-vivo.
- an array detector e.g. CCD 18
- the breast tissue 12 is illuminated with two eLEDs 16 which in an exemplary embodiment are located 60 mm away from each other (15 mm away from either side of the CCD 18 ) and are used to have symmetrical light illumination sources.
- FIGS. 1 and 2 present a sketch and photograph of the DOB-Scan probe 10 , respectively.
- the linear CCD 18 and light illumination sources e.g. eLEDs 16
- the diffusion of light in the selected wavelengths are maximum due to minimum absorption of breast fat.
- An exemplary embodiment of the DOB-Scan probe 10 for functional and compositional cross-sectional imaging of the breast tissue 12 employs multi-wavelength diffuse optical spectroscopy technique with four wavelengths encapsulated in an eLED 16 light source in the near infrared spectrum range.
- the device and associated system hardware provide four two-dimensional images of the breast tissue's function and composition.
- Each eLED 16 contains four wavelengths (e.g. 690 nm, 750 nm, 800 nm and 850 nm), which are illuminated into the breast tissue 12 once a time and the linear CCD 18 collects scattered light on the skin of the breast tissue 12 . Then, the system processes the data and transfers them to a host computer 20 for further analysis and image reconstruction.
- the system is able to create one or more cross-sectional optical absorption images for concentration of one or more different chromophores in the breast tissue such as Hb and HbO 2 which are markers for cancerous tissue.
- the prototypical probe and method accurately create cross-sectional optical absorption images such that there is no need to use laser diodes or fiber coupled laser diodes as an illumination source, although such illumination sources may be employed in variations of embodiments of the present invention.
- Real time data acquisition system components add functional imaging advantages to the probe.
- the image reconstruction for each wavelength is performed at the rate of 24 Hz which is sufficient for functional imaging of biological activity.
- FIG. 3 A photograph of the DOB-Scan probe 10 prototype with its system hardware housed within the DOB-Scan probe 10 is shown in FIG. 3 .
- the host computer 20 includes a processor and memory in a manner known in the art. While FIG. 3 shows a wired connection 22 between the DOB-Scan probe 10 and the host computer 20 , in some embodiments the DOB-Scan probe 10 is battery-powered and operable to communicate with the host computer 20 by any suitable wireless communications technology (e.g. BluetoothTM or Wi-Fi).
- any suitable wireless communications technology e.g. BluetoothTM or Wi-Fi
- FIG. 4 A Schematic diagram of the instrument is shown in FIG. 4 , including showing a power management unit and battery charger 24 , a battery 26 , and a wireless communication unit 28 .
- the wireless communication unit 28 may implement BluetoothTM, Wi-Fi and/or other wireless communications technology for example.
- Each wavelength is illuminated in the breast tissue 12 once a time and the linear CCD 18 collects scattered light on the skin of the breast tissue and converts it to analogue voltage. Then, analog signals are converted to digital signals and passed to the main processor.
- the microcontroller unit (MCU) 30 is responsible to create control signals for illumination sources (e.g. eLEDs 16 ), analog to digital convertor (ADC) 32 and CCD 18 .
- the MCU 30 reads digital data from ADC 32 and transfers data to host computer 20 for further analysis, as well.
- Two eLEDs 16 are used in the probe 10 , which are located 15 mm away from each end-side of the detector (e.g. CCD 18 ) as shown in FIG. 1 .
- Each BLED 16 includes four wavelengths: 690 nm, 750 nm, 800 nm and 850 nm.
- the probe has eight LEDs which can turn on and off individually or sequentially by the time period of 200 milliseconds. Intensity of each LED can be calibrated by trimmer potentiometer on the instrument (e.g. DOB-Scan probe 10 ).
- the light detector plays an important role in the instrument. In order to have high resolution images, it should have large number of light sensors with small size and patches.
- the light sources are located at two ends of the detector and the illuminated photons are travelling in different path lengths to reach to the photodetectors.
- the photodetectors which are located close to the active light source will be exposed to a higher light intensity and the ones that are placed far from active light source, will receive lower light intensity. Therefore, the photodetectors must have a large dynamic range to allow measurement for both small and large source-photodetector separations.
- the photodetector circuit of the prototype is specially designed to address these challenges.
- the light detector (ILX511, SONYTM) used for the probe 10 is a linear charge coupled device 18 , which has a fixed capacitor associated with each photodetector (pixel).
- the photodetector chip of the prototype has 2086 pixels in total which is called one frame. 3.2 pixels at the beginning of the frame and 6 pixels at the end of the frame are dummy pixels. Therefore, the photodetector of the prototype has 2048 effective pixels with 14 ⁇ m pixel pitch; consequently the effective imaging area of the prototype is 28.672 mm (2048 ⁇ 14 ⁇ m).
- NIR photons migration in biological tissue and optical properties of the breast tissue are compatible with diffusion optical spectroscopy.
- Four main absorbers in the breast tissue in NIR range (650 nm to 1100 nm) are deoxyhemoglobin (Hb), oxyhemoglobin (HbO 2 ), water and fat.
- Hb deoxyhemoglobin
- HbO 2 oxyhemoglobin
- the optical properties of the breast tissue are widely studied and published in academic journal articles. These studies demonstrate that absorption of water and fat are low in NIR range, which allows photons to travel deeply into the breast tissue.
- the propagation of the light in a highly scattering medium like the breast tissue 12 can be precisely modeled utilising the diffusion equation. As illustrated in FIG. 1 , source-detector separations in the DOB-Scan probe 10 vary from 15 mm to 43.672 rm. Therefore, illuminated light into the breast tissue pass different depth and path in the breast tissue to reach to the detectors. The measured photons travel longer path and deeper in the breast tissue 12 while source-detector separation is wider.
- ⁇ a absorption coefficient ( ⁇ a ) of the paths photons travel in the breast tissue 12 .
- a closed form solution for the diffusion equation in a semi-infinite homogeneous medium may be employed while light source and detector are located in spatially reflectance geometry.
- the extracted absorption coefficients for photon paths are used to create two-dimensional cross-sectional image for each NIR wavelengths.
- the radiative transfer equation can be simplified by the diffusion equation as follows:
- ⁇ d represents the intensity at the detector located r mm away from the illumination source(S(r)).
- c m and ⁇ a are the photons velocity in the medium and absorption coefficient, respectively.
- D represents diffusion coefficient.
- the photons' migration in the biological tissue is in the scattering region and diffusion equation can be used to describe photons propagation.
- the following expression (2) presents analytical solution for the diffusion equation in a homogeneous media while the illumination source and detector setting in reflectance geometry.
- the diffusion equation is effective while r is greater than 10 ⁇ ( ⁇ a + ⁇ ′ s ) ⁇ 1 and ⁇ a « ⁇ ′ s .
- Oxy-hemoglobin (HbO 2 ), deoxy-hemoglobin (Hb), water (H 2 O), and fat are the four primary chromophores in the breast tissue which contribute to the attenuation at wavelength ⁇ and concentration of HbO 2 and Hb vary by the time.
- the direct-approach-method equation (3) can be used to create cross-sectional images for the four chromophores concentration and concentration variations in Hb ( ⁇ cHb) and HbO 2 ( ⁇ cHbO 2 ) shown in the cross-sectional images.
- ⁇ n ⁇ is the molar absorption extinction coefficient for the chromophore type n (n should be Hb, HbO 2 , H 2 O and Fat) at illuminated wavelength of ⁇ (690 nm, 750 nm, 800 nm, 850 nm) and C, is the total concentration of the chromophore in the photons path lengths. While the exemplary wavelengths of 690, 750, 800 and 850 nm are mentioned herein in association with the chromophores of Hb, HbO 2 , H 2 O and Fat, it is understood that each of the wavelengths may vary in any range that is suitably associated with a corresponding chromophore or other human-tissue constituent or type.
- the obtained raw data from the exemplary prototype having 2048 pixels, 2 sources and 4 wavelengths are measurement points collected from surface of the tissue.
- a reconstruction algorithm is useful for converting the surface measured light intensity into two-dimensional cross-sectional images of the chromophores.
- the absorption coefficient ( ⁇ a ) of each light paths are calculated by utilizing equation (2) while the tissue is illuminated by each wavelength.
- Table 1 presents a brief specification of the exemplary DOB-Scan probe in prototype form.
- the software interface communicates with the DOB-Scan probe 10 via USB2.0 (or other serial communications technology) virtual comport and contains a graphical user interface (GUI) 34 , as shown in FIG. 3 , for operator's ease of use.
- GUI 34 is comprised of a series of tabs and collected data graphs, which are used for various operations including: Manual and periodic light source selection, CCD 18 integration time setting and data collection.
- the user Upon launching the software interface the user must select illuminated wavelengths manually or set it periodically in LEDs control panel.
- 24 frames per second are collected from the image sensor, which it is sufficient for functional imaging of biological tissue.
- periodic mode each wavelength is illuminated for 200 milliseconds and the data are collected with the same period. In this mode, eight frames are collected sequentially while different wavelengths are illuminated into the biological tissue, and in order to measure ambient light effect on the image sensor, one more frame is collected while all the LEDs are turned off.
- phantom #1 14 in which the hole 36 was filled with tumor-like liquid phantom.
- the liquid-phantom is a solution consisting of 1 L water, 50 mL Intralipid® and 1.5 mL India black ink.
- the probe 10 in three different locations on the phantom 14 in which the middle line of the probe 10 (middle of the CCD 18 ) is aligned: a) along the center of the hole 36 , b) 7 mm to the left of the hole 36 center, and c) 7 mm to the right of the hole 36 center, as shown in FIGS. 7 a , 7 b , and 7 c , respectively.
- FIGS. 8 a , 8 b , and 8 c show the reconstructed cross-sectional images 38 of the phantom for the wavelength of 690 nm while the breast phantom's hole 36 contains the India black ink solution and while the probe 10 is located: a) along the center of the hole 36 , b) 7 mm to the left of the hole 36 center, and c) 7 mm to the right of the hole 36 center.
- the reddish area 40 on the images shows the location of the hole 36 on the phantom, in contrast to differently colored areas such as the non-reddish area(s) 42 .
- FIGS. 9 a , 9 b , and 9 c show cross-sectional images 44 while the probe 10 is placed on different locations of the phantom while the 690 nm light source is illuminated.
- FIGS. 9 a , 9 b , and 9 c show reddish area(s) 40 corresponding to the locations (centrally aligned, to the left, and to the right) of the breast phantom's hole 36 containing the PAR, respectively, in contrast to non-reddish area(s) 42 that can be seen in FIGS. 9 a , 9 b , and 9 c .
- the probe 10 did not capture the inhomogeneity due to similar absorption coefficient of RBSA with surrounded material as demonstrated by the images 46 shown in FIGS.
- FIGS. 10 a , 10 b , and 10 c for the wavelength of 800 nm in which a reddish area 40 is not visible.
- the three cross-sectional images of FIGS. 10 a , 10 b , and 10 c produced by use of the DOB-Scan probe 10 show an absence of reddish areas 40 corresponding to the location of the breast phantom's hole containing the PAR when the prototypical handheld probe 10 is emitting electromagnetic radiation at 800 nm and is located: a) along the center of the hole 36 , b) to the left of the hole 36 center, and c) to the right of the hole 36 center.
- only non-reddish areas 42 are predominantly visible in FIGS. 10 a , 10 b , 10 c.
- FIGS. 11 a to 11 f are six cross-sectional images produced by the probe 10 showing reddish areas 40 corresponding to the location of the breast phantom's hole containing varying concentrations of India black ink when the handheld probe 10 is emitting electromagnetic radiation at 690 nm.
- the performance of an exemplary prototype of the probe 10 was evaluated by testing it on breast tissue-like phantom 14 .
- the test results of the exemplary probe being used on breast tissue-like phantom show that the probe 10 can create functional images and chromophores composition images of the phantom 14 .
- Extended data collected for cancerous tissue further demonstrates the probe's performance in its prototypical form, A technique to identify malignant breast tumors using contralateral healthy body tissue as a control is used to produce preliminary results for ten cases presented below.
- the reconstructed preliminary images show that the DOB-San probe can distinguish the cancerous region from the surrounding healthy tissue.
- the cancerous region is observable in the images (red area) due to a higher level of absorption caused by higher levels of vascularization.
- the red area on the images reflexes location of anomaly tissue in the breast.
- Table 2 represents brief clinical reports for the patients who enrolled in the further clinical study.
- FIGS. 12 a to 12 h , 13 a to 13 h , 14 a to 14 h , 15 a to 15 h , 16 a to 16 h , 17 a to 17 h , 18 a to 18 h , 19 a to 19 h , and 20 a to 20 h represent reconstructed images 50 for four different wavelengths while the probe 10 positioned on the location where diagnosed having a cancerous lesion and its contralateral position for 9 of the 10 patients tabulated above.
- the reddish area 40 on the images reflect the location of anomalous tissue, in contrast to the non-reddish areas 42 .
- each breast has four images for four different wavelengths.
- Table 3 indicates the parameters associated with the Figures identified as “a” to “h” for each the Figure sets enumerated by the numbers 12 to 20 ,
- FIG. 12 a , FIG. 13 a , and so on to FIG. 20 a are each associated with the wavelength 690 nm and the left breast
- FIG. 12 b , FIG. 13 b , and so on to FIG. 20 h are each associated with 690 nm and the right breast.
- Case 1 There was two cancerous lesions in the left breast and the probe 10 located on the one located at 10 o'clock of the left breast.
- FIGS. 12 a to 12 h present that the probe 10 captured the tumor correctly.
- There are some reddish areas 40 on the healthy breast FIGS. 12 b , 12 d , 12 f , and 12 h ) which caused by saturation of the detector.
- Case 2 There was two cancerous lesions in the left breast and the probe 10 located on the one located at 4 o'clock of the left breast.
- FIGS. 13 a to 13 h present that the probe 10 captured the tumor correctly and it is in line with mammography images.
- There are some reddish areas 40 on the healthy breast FIGS. 13 b , 13 d , 13 f , and 13 h ) which caused by saturation of the detector.
- FIGS. 14 a to 14 h presents that the probe 10 captured the tumor correctly and it is in line with mammography images. There are some reddish areas 40 on the healthy breast ( FIGS. 14 b , 14 d , 14 f , and 14 h ) which caused by saturation of the detector.
- Case 4 There was a cancerous lesions in the right breast at 9 o'clock. This patient had large breasts with a cancerous lesion positioned near to the chest wall. In these cases, the probe 10 captures the cancerous lesion with difficulty due to the depth of the tumor in the breast ( FIGS. 15 b , 15 d , 15 f , and 15 h ). There are some reddish areas 40 on the healthy breast ( FIGS. 15 a , 15 c , 15 e , and 15 g ) which caused by saturation of the detector.
- Case 5 There was a cancerous lesions in the left breast at 2 o'clock.
- Reconstructed images for the left breast of patient #5 ( FIGS. 16 a , 16 c , 16 e , and 16 g ) show that the absorption coefficient of the whole breast is unusually higher than for the malignant tissue.
- Medical advice from the patient's oncologists indicates there was a lobulated irregular large shadowing mass which had spread all over the left breast. For this reason, the probe 10 shows higher absorption coefficient whole over the breast.
- There are some reddish areas 40 on the healthy breast FIGS. 16 b , 16 d , 16 f , and 16 h ) which caused by saturation of the detector.
- FIGS. 17 a to 17 h present that the probe 10 captured the tumor correctly and it is in line with mammography images. There are some reddish areas 40 on the healthy breast which shows a mass in healthy tissue that has a higher absorption coefficient at 800 nm ( FIG. 17 e ) and 850 nm ( FIG. 17 g ). According to FIGS. 17 a to 17 h , water and fat have higher absorption coefficients than the other two absorbers in breast tissue. Medical advice from the patients oncologists indicates there was a benign mass which is filled with liquid at the location.
- Case 7 There was a cancerous lesions in the right breast between 10 and 11 o'clock.
- FIGS. 18 a to 18 h present that the probe 10 captured the tumor correctly and it is in line with mammography images.
- the DOB-Scan probe's images also show a mass in healthy tissue ( FIGS. 18 a . 18 c , 18 e , and 18 g ) that has a higher absorption coefficient.
- Medical advice from the patient's oncologists indicates there was a benign mass which filled with liquid at the location.
- Case 9 There was a cancerous lesions in the left breast at 1 o'clock.
- FIGS. 19 a to 19 h present that the probe 10 captured the tumor correctly and it is in line with mammography images.
- FIGS. 20 a to 20 h present that the probe 10 captured the tumor correctly and it is in line with mammography images.
- the DOB-Scan probe 10 can be used to capture cross-sectional images of the female breast's interior using NIR light sources associated with a linear CCD.
- the reconstructed preliminary images show that DOB-San probe can identify the cancerous region from the surrounding healthy tissue.
- the cancerous region is observed in the images due to the higher level of absorption caused by higher levels of vascularization in the cancerous region.
- a diffuse-optical-spectroscopy system for scanning human tissue.
- the system includes: (a) a handheld probe operable to emit electromagnetic radiation at one or more wavelengths corresponding to absorption associated with one or more human-tissue constituents, respectively, the handheld probe being operable to detect received electromagnetic radiation at each of the one or more wavelengths: and (b) a processor operable to produce, in response to the received electromagnetic radiation, one or more cross-sectional images of the human tissue respectively associated with the one or more wavelengths.
Abstract
A diffuse-optical-spectroscopy system and method for scanning human tissue is provided. The system includes: (a) a handheld probe operable to emit electromagnetic radiation at one or more wavelengths corresponding to absorption associated with one or more human-tissue constituents, respectively, the handheld probe being operable to detect received electromagnetic radiation at each of the wavelengths; and (b) a processor operable to produce, in response to the received electromagnetic radiation, one or more cross-sectional images of the human tissue respectively associated with the wavelengths.
The handheld probe includes first and second sources for emitting the electromagnetic radiation and one or more sensors for detecting the received electromagnetic radiation. The sensors are aligned along a first axis and face in an outward direction. The first and second sources are aligned along the first axis, face in the outward direction, and are disposed on either side of the sensors.
Description
- This invention relates to diffusion optical spectroscopy and, in particular, to a handheld probe and system using the handheld probe for cross-sectional imaging of human tissue.
- According to the Canadian and American cancer societies' reports, breast cancer is the most common cancer in women and it is ranked as the second cause of death, in North America. Breast cancer affects approximately one in eight North America women during their lifetime. Treatment entails a number of steps from the time of diagnoses to a consistent follow-up after the treatment stage. These steps have to be taken seriously if there is any suspicions lesion. Introducing new technology or improving one or more of the assessment parameters including: accuracy, sensitivity, specificity or positive predictive value on current techniques, may help physicians to detect breast cancer in its early stage, which is crucial in women's health. The screening methods of clinical breast exam (CBE) and X-ray mammography are questionable for their lower sensitivity. X-ray mammography is a preliminary screening methodology, it is painful and uses harmful ionizing X-ray radiation.
- Ultrasound, x-ray mammography, and magnetic resonance imaging (MRI) are commonly used for breast cancer detection where X-ray mammography is the primary screening technique for breast cancer. Although X-ray mammography is the principle modality for breast cancer screening, it is recommended for women over the age of 50, because it has low sensitivity (67.8%) for younger women or women with dense breasts. In addition, it has a potential health risk as an effect of ionizing radiations. Therefore, because of X-ray mammography's limitations, researchers have been encouraged to use different breast cancer detection modalities, such as diffuse optical tomography, impedance spectroscopy and elasto-graphy.
- Over the past three decades great progress has occurred in optoelectronics components and fiber optics. In parallel with these developments, near-infrared (NIR) optical imaging, in association with optoelectronic devices and fiber optics, also have been developed very rapidly and currently NIR optical imaging approaches have been advanced for breast cancer diagnosis. This technique is known as a noninvasive and promising method for compositional and functional imaging of extremely scattering mediums, such as brain and breast.
- The optical properties of the breast tissue have been widely studied. For example, it is known that the absorption of the four main constituents of breast tissue, namely water, fat, deoxyhemoglobin (Hb) and oxyhemoglobin (HbO2), is low in the wavelength spectrum from 650 nm to 1100 nm, which allows light to travel deeply into the breast tissue.
- A number of handheld diffuse optical probes have been implemented and tested for breast cancer detection and screening. Almost all of the probes are designed in reflectance geometry to measure optical properties of the breast tissue underneath of the probes. Laser light sources or fiber coupled laser light sources in near infrared range are used to determine the concentration of main chromophores in the breast tissue related to sensing anomalies. However, laser and fiber-optic based illumination techniques are complex, large in size and costly.
- An object of the invention is to address the above shortcomings.
- The above shortcomings may be addressed by providing, in accordance with one aspect of the invention, a diffuse-optical-spectroscopy system for scanning human tissue. The system includes: (a) a handheld probe operable to emit electromagnetic radiation at one or more wavelengths corresponding to absorption associated with one or more human-tissue constituents, respectively, the handheld probe being operable to detect received electromagnetic radiation at each of the one or more wavelengths; and (b) a processor operable to produce, in response to the received electromagnetic radiation, one or more cross-sectional images of the human tissue respectively associated with the one or more wavelengths.
- The handheld probe may include one or more sensors for detecting the received electromagnetic radiation, the one or more sensors being aligned along a first axis and facing in an outward direction. The handheld probe may include first and second sources for emitting the electromagnetic radiation, the first and second sources being aligned along the first axis, facing in the outward direction, and disposed on either side of the one or more sensors. The one or more wavelengths may include at least four wavelengths corresponding to absorption associated with at least four human-tissue constituents. Each of the at least four wavelengths may be in at least one of a visible region, a near-infrared region, and an infrared region of the electromagnetic spectrum. The at least four human-tissue constituents may include deoxyhemoglobin, oxyhemoglobin, water and fat. Each of the first and second sources may include first, second, third and fourth light sources operable to emit the electromagnetic radiation at first, second, third and fourth wavelengths, respectively, each of the first, second, third and fourth light sources being selected from the group consisting of a light-emitting diode, an encapsulated light-emitting diode, and a laser. The handheld probe may include a linear array detector (charge-coupled device or linear photodiode) that includes the one or more sensors: The electromagnetic radiation on may be continuous wave electromagnetic radiation.
- In accordance with another aspect of the invention, there is provided a method of scanning human tissue by diffuse-optical-spectroscopy. The method involves: (a) emitting electromagnetic radiation at one or more wavelengths corresponding to absorption associated with one or more human-tissue constituents, respectively, by a handheld probe placed in proximity to the human tissue: (b) detecting by the handheld probe received electromagnetic radiation at each of the one or more wavelengths; and (c) in response to the received electromagnetic radiation, producing by a processor one or more cross-sectional images of the human tissue respectively associated with the one or more wavelengths.
- Step (b) of the method may involve detecting the received electromagnetic radiation by one or more sensors of the handheld probe when the one or more sensors are aligned along a first axis and facing in an outward direction. Step (a) of the method may involve emitting the electromagnetic radiation by first and second sources of the handheld probe when the first and second sources are aligned along the first axis, facing in the outward direction, and disposed on either side of the one or more sensors. Step (a) of the method may involve emitting the electromagnetic radiation at the one or more wavelengths that may include at least four wavelengths corresponding to absorption associated with at least four human-tissue constituents, each of the at least four wavelengths being in at least one of a visible region, a near-infrared region, and an infrared region of the electromagnetic spectrum, the at least four human-tissue constituents comprising deoxyhemoglobin, oxyhemoglobin, water and fat. Step (a) of the method may involve emitting the electromagnetic radiation by first, second, third and fourth light sources at first, second, third and fourth wavelengths, respectively when each of the first, second., third and fourth light sources are selected from the group consisting of a light-emitting diode, an encapsulated light-emitting diode, and a laser. Step (b) of the method may involve detecting the received electromagnetic radiation by at least one of a charge-coupled device and an array photodiode of the handheld probe when the at least one of a charge-coupled device and an array photodiode comprises the one or more sensors. Step (a) of the method may involve emitting continuous wave electromagnetic radiation.
- In accordance with another aspect of the invention, there is provided a system for scanning human tissue by diffuse-optical-spectroscopy. The system includes: (a) source means for emitting electromagnetic radiation at one or more wavelengths corresponding to absorption associated with one or more human-tissue constituents, respectively; (b) receiving means for detecting received electromagnetic radiation at each of the one or more wavelengths; and (c) processing means for producing, in response to the received electromagnetic radiation, one or more cross-sectional images of the human tissue respectively associated with the one or more wavelengths.
- Diffuse optical spectroscopy (DOS) and diffuse optical Imaging (DCI) are methods for breast cancer diagnosis which are noninvasive and nonionizing techniques. The DOB-Scan probe is used to measure optical properties of breast tissue and create functional and compositional cross-sectional images of the breast. Four wavelengths light emitting diodes (LED), encapsulated in a package (eLED), are used to illuminate the breast tissue. A linear charge coupled device (CCD) measures the intensity of the scattered photons at different radial destinations from the illumination source on the surface of the breast tissue. The proposed method replaces fiber optic based illumination techniques, which increases the complexity, size and cost of a potential probe; by multi-wavelengths eLED which acts as a pencil beam source in such a scattering media like the breast tissue. In addition, the DOB-Scan probe and associated algorithm can create cross-sectional images of the breast tissue underneath of the probe by using high resolution linear CCD. The introduced technique miniaturizes the probe, while maintaining reliability and accuracy of this technique in breast imaging. Multi-wavelength light sources are used to increase the capability of the probe by creating separate images for four primary chromophores of the breast tissue including deoxyhemoglobin, oxyhemoglobin, fat and water. The average scattering coefficient of the medium and localized concentration variations in oxyhemoglobin and deoxyhemoglobin can be measured utilizing the probe. In order to evaluate the performance of DOB-Scan probe, a series of tissue-like materials (phantoms), containing of Intralipid®, Black ink, Defrin®, and Pierce™ have been used to produce the experimental results indicated below. The test results provide evidence that the DOB-Scan probe can detect any inhomogeneity inside the phantoms.
- The foregoing summary is illustrative only and is not intended to be in any way limiting. Other aspects and features of the present invention will become apparent to those of ordinary skill in the art upon review of the following description of embodiments of the invention in conjunction with the accompanying figures and claims.
- In drawings which illustrate by way of example only embodiments of the invention:
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FIG. 1 is a schematic representation of a handheld probe according to a first embodiment of the invention, showing sources and a detector; -
FIG. 2 is a photograph of the prototypical handheld probe shown inFIG. 1 , showing locations of first and second eLEDs and a linear CCD; -
FIG. 3 is a photograph of a prototypical system according to the first embodiment, showing the prototypical handheld probe connected to a host computer; -
FIG. 4 is a schematic representation of the system shown inFIG. 3 , showing a wireless communication unit; -
FIG. 5 is a schematic representation of the handheld probe shown inFIG. 1 , showing a highly scattering medium; -
FIG. 6 is a photograph of a breast phantom for use in testing the prototypical system shown inFIG. 3 , showing a hole in the breast phantom for containing liquid providing a phantom representation of a cancerous tumor: -
FIG. 7 a is a photograph of the breast phantom ofFIG. 6 and the prototypical system ofFIG. 3 , showing the prototypical handheld probe's middle placed along the center of the hole of the breast phantom containing India black ink; -
FIG. 7 b is a photograph of the breast phantom ofFIG. 6 and the prototypical system ofFIG. 3 , showing the prototypical handheld probe's middle placed to the left of the hole of the breast phantom containing India black ink; -
FIG. 7 c is a photograph of the breast phantom ofFIG. 6 and the prototypical system ofFIG. 3 , showing the prototypical handheld probe's middle placed to the right of the hole of the breast phantom containing India black ink; -
FIG. 8 a is a cross-sectional image produced by the prototypical system shown inFIG. 3 when the probe is emitting electromagnetic radiation at 690 nm and is located along the center of the breast phantom's hole containing India black ink; -
FIG. 8 b is a cross-sectional image produced by the prototypical system shown inFIG. 3 when the probe is emitting electromagnetic radiation at 690 nm and is located to the left of the breast phantom's hole containing India black ink; -
FIG. 8 c is a cross-sectional image produced by the prototypical system shown inFIG. 3 when the probe is emitting electromagnetic radiation at 690 nm and is located to the right of the breast phantom's hole containing India black ink; -
FIG. 9 a is a cross-sectional image produced by the prototypical system shown inFIG. 3 when the probe is emitting electromagnetic radiation at 690 nm and is located along the center of the breast phantom's hole containing a protein assay reagent; -
FIG. 9 b is a cross-sectional image produced by the prototypical system shown inFIG. 3 when the probe is emitting electromagnetic radiation at 690 nm and is located to the left of the breast phantom's hole containing the protein assay reagent; -
FIG. 9 c is a cross-sectional image produced by the prototypical system shown inFIG. 3 when the probe is emitting electromagnetic radiation at 690 nm and is located to the right of the breast phantom's hole containing the protein assay reagent; -
FIG. 10 a is a cross-sectional image produced by the prototypical system shown inFIG. 3 when the probe is emitting electromagnetic radiation at 800 nm and is located along the center of the breast phantom's hole containing the protein assay reagent; -
FIG. 10 b is a cross-sectional image produced by the prototypical system shown inFIG. 3 when the probe is emitting electromagnetic radiation at 800 nm and is located to the left of the breast phantom's hole containing the protein assay reagent: -
FIG. 10 c is a cross-sectional image produced by the prototypical system shown inFIG. 3 when the probe is emitting electromagnetic radiation at 800 nm and is located to the right of the breast phantom's hole containing the protein assay reagent; -
FIG. 11 a is a cross-sectional image produced by the prototypical system shown inFIG. 3 , showing reddish areas or lack thereof corresponding to the location of the breast phantom's hole containing a reference solution with India black ink in the concentration of 0.125 ml/l when the prototypical handheld probe is emitting electromagnetic radiation at 690 nm; -
FIG. 11 b is a cross-sectional image produced by the prototypical system shown inFIG. 3 , showing reddish areas or lack thereof corresponding to the location of the breast phantom's hole containing the reference solution with India black ink in the concentration of 0.25 ml/l when the prototypical handheld probe is emitting electromagnetic radiation at 690 nm; -
FIG. 11 c is a cross-sectional image produced by the prototypical system shown inFIG. 3 , showing reddish areas or lack thereof corresponding to the location of the breast phantom's hole containing the reference solution with India black ink in the concentration of 0.375 ml/l when the prototypical handheld probe is emitting electromagnetic radiation at 690 nm; -
FIG. 11 d is a cross-sectional image produced by the prototypical system shown inFIG. 3 , showing reddish areas or lack thereof corresponding to the location of the breast phantom's hole containing the reference solution with India black ink in the concentration of 0.5 ml/l when the prototypical handheld probe is emitting electromagnetic radiation at 690 nm; -
FIG. 11 e is a cross-sectional image produced by the prototypical system shown inFIG. 3 , showing reddish areas or lack thereof corresponding to the location of the breast phantom's hole containing the reference solution with India black ink in the concentration of 0.625 ml/I when the prototypical handheld probe is emitting electromagnetic radiation at 690 nm; -
FIG. 11 f is a cross-sectional image produced by the prototypical system shown inFIG. 3 , showing reddish areas or lack thereof corresponding to the location of the breast phantom's hole containing the reference solution with India black ink in the concentration of 0.75 ml/l when the prototypical handheld probe is emitting electromagnetic radiation at 690 nm; -
FIG. 12 a is a sectional image associated withpatient case 1, a wavelength of 690 nm, and the left breast ofpatient 1; -
FIG. 12 b is a sectional image associated withpatient case 1, a wavelength of 690 nm, and the right breast ofpatient 1; -
FIG. 12 c is a sectional image associated withpatient case 1, a wavelength of 750 nm, and the left breast ofpatient 1; -
FIG. 12 d is a sectional image associated withpatient case 1, a wavelength of 750 nm, and the right breast ofpatient 1; -
FIG. 12 e is a sectional image associated withpatient case 1, a wavelength of 800 nm, and the left breast ofpatient 1; -
FIG. 12 f is a sectional image associated withpatient case 1, a wavelength of 800 nm, and the right breast ofpatient 1; -
FIG. 12 g is a sectional image associated withpatient case 1, a wavelength of 850 nm, and the left breast ofpatient 1; -
FIG. 12 h is a sectional image associated withpatient case 1, a wavelength of 850 nm, and the right breast ofpatient 1; -
FIG. 13 a is a sectional image associated withpatient case 2, a wavelength of 690 nm, and the left breast ofpatient 2; -
FIG. 13 b is a sectional image associated withpatient case 2, a wavelength of 690 nm, and the right breast ofpatient 2; -
FIG. 13 c is a sectional image associated withpatient case 2, a wavelength of 750 nm, and the left breast ofpatient 2; -
FIG. 13 d is a sectional image associated withpatient case 2, a wavelength of 750 nm, and the right breast ofpatient 2; -
FIG. 13 e is a sectional image associated withpatient case 2, a wavelength of 800 nm, and the left breast ofpatient 2; -
FIG. 13 f is a sectional image associated withpatient case 2, a wavelength of 800 nm, and the right breast ofpatient 2; -
FIG. 13 g is a sectional image associated withpatient case 2, a wavelength of 850 nm, and the left breast ofpatient 2; -
FIG. 13 h is a sectional image associated withpatient case 2, a wavelength of 850 nm, and the right breast ofpatient 2; -
FIG. 14 a is a sectional image associated withpatient case 3, a wavelength of 690 nm, and the left breast ofpatient 3; -
FIG. 14 b is a sectional image associated withpatient case 3, a wavelength of 690 nm, and the right breast ofpatient 3; -
FIG. 14 c is a sectional image associated withpatient case 3, a wavelength of 750 nm, and the left breast ofpatient 3; -
FIG. 14 d is a sectional image associated withpatient case 3, a wavelength of 750 nm, and the right breast ofpatient 3; -
FIG. 14 e is a sectional image associated withpatient case 3, a wavelength of 800 nm, and the left breast ofpatient 3; -
FIG. 14 f is a sectional image associated withpatient case 3, a wavelength of 800 nm, and the right breast ofpatient 3; -
FIG. 14 g is a sectional image associated withpatient case 3, a wavelength of 850 nm, and the left breast ofpatient 3; -
FIG. 14 h is a sectional image associated withpatient case 3, a wavelength of 850 nm, and the right breast ofpatient 3; -
FIG. 15 a is a sectional image associated withpatient case 4, a wavelength of 690 nm, and the left breast ofpatient 4; -
FIG. 15 b is a sectional image associated withpatient case 4, a wavelength of 690 nm, and the right breast ofpatient 4; -
FIG. 15 c is a sectional image associated withpatient case 4, a wavelength of 750 nm, and the left breast ofpatient 4; -
FIG. 15 d is a sectional image associated withpatient case 4, a wavelength of 750 nm, and the right breast ofpatient 4; -
FIG. 15 e is a sectional image associated withpatient case 4, a wavelength of 800 nm, and the left breast ofpatient 4; -
FIG. 15 f is a sectional image associated withpatient case 4, a wavelength of 800 nm, and the right breast ofpatient 4; -
FIG. 15 g is a sectional image associated withpatient case 4, a wavelength of 850 nm, and the left breast ofpatient 4; -
FIG. 15 h is a sectional image associated withpatient case 4, a wavelength of 850 nm, and the right breast ofpatient 4; Figure a is a sectional image associated withpatient case 5, a wavelength of 690 nm, and the left breast ofpatient 5; -
FIG. 6 b is a sectional image associated withpatient case 5, a wavelength of 690 nm, and the right breast ofpatient 5; -
FIG. 16 c is a sectional image associated withpatient case 5, a wavelength of 750 nm, and the left breast ofpatient 5; -
FIG. 16 d is a sectional image associated withpatient case 5, a wavelength of 750 nm, and the right breast ofpatient 5; -
FIG. 16 e is a sectional image associated withpatient case 5, a wavelength of 800 nm, and the left breast ofpatient 5; -
FIG. 16 f is a sectional image associated withpatient case 5, a wavelength of 800 nm, and the right breast ofpatient 5; -
FIG. 16 g is a sectional image associated withpatient case 5, a wavelength of 850 nm, and the left breast ofpatient 5; -
FIG. 16 h is a sectional image associated withpatient case 5, a wavelength of 850 nm, and the right breast ofpatient 5; -
FIG. 17 a is a sectional image associated withpatient case 6, a wavelength of 690 nm, and the left breast ofpatient 6; -
FIG. 17 b is a sectional image associated withpatient case 6, a wavelength of 690 nm, and the right breast ofpatient 6; -
FIG. 17 c is a sectional image associated withpatient case 6, a wavelength of 750 nm, and the left breast ofpatient 6; -
FIG. 17 d is a sectional image associated withpatient case 6, a wavelength of 750 nm, and the right breast ofpatient 6; -
FIG. 17 e is a sectional image associated withpatient case 6, a wavelength of 800 nm, and the left breast ofpatient 6; -
FIG. 17 f is a sectional image associated withpatient case 6, a wavelength of 800 nm, and the right breast ofpatient 6; -
FIG. 17 g is a sectional image associated withpatient case 6, a wavelength of 850 nm, and the left breast ofpatient 6; -
FIG. 17 h is a sectional image associated withpatient case 6, a wavelength of 850 nm, and the right breast ofpatient 6; Figure a is a sectional image associated with patient case 7, a wavelength of 690 nm, and the left breast of patient 7; -
FIG. 8 b is a sectional image associated with patient case 7, a wavelength of 690 nm, and the right breast of patient 7; -
FIG. 18 c is a sectional image associated with patient case 7, a wavelength of 750 nm, and the left breast of patient 7; -
FIG. 18 d is a sectional image associated with patient case 7, a wavelength of 750 nm, and the right breast of patient 7; -
FIG. 18 e is a sectional image associated with patient case 7, a wavelength of 800 nm, and the left breast of patient 7; -
FIG. 18 f is a sectional image associated with patient case 7, a wavelength of 800 nm, and the right breast of patient 7; -
FIG. 18 g is a sectional image associated with patient case 7, a wavelength of 850 nm, and the left breast of patient 7; -
FIG. 18 h is a sectional image associated with patient case 7, a wavelength of 850 nm, and the right breast of patient 7; -
FIG. 19 a is a sectional image associated with patient case 9, a wavelength of 690 nm, and the left breast of patient 9; -
FIG. 19 b is a sectional image associated with patient case 9, a wavelength of 690 nm, and the right breast of patient 9; -
FIG. 19 c is a sectional image associated with patient case 9, a wavelength of 750 nm, and the left breast of patient 9; -
FIG. 19 d is a sectional image associated with patient case 9, a wavelength of 750 nm, and the right breast of patient 9; -
FIG. 19 e is a sectional image associated with patient case 9, a wavelength of 800 nm, and the left breast of patient 9; -
FIG. 19 f is a sectional image associated with patient case 9, a wavelength of 800 nm, and the right breast of patient 9; -
FIG. 19 g is a sectional image associated with patient case 9, a wavelength of 850 nm, and the left breast of patient 9; -
FIG. 19 h is a sectional image associated with patient case 9, a wavelength of 850 nm, and the right breast of patient 9; -
FIG. 20 a is a sectional image associated withpatient case 10, a wavelength of 690 nm, and the left breast ofpatient 10; -
FIG. 20 b is a sectional image associated withpatient case 10, a wavelength of 690 nm, and the right breast ofpatient 10; -
FIG. 20 c is a sectional image associated withpatient case 10, a wavelength of 750 nm, and the left breast ofpatient 10; -
FIG. 20 d is a sectional image associated withpatient case 10, a wavelength of 750 nm, and the right breast ofpatient 1 -
FIG. 20 e is a sectional image associated withpatient case 10, a wavelength of 800 nm, and the left breast ofpatient 10; -
FIG. 20 f is a sectional image associated withpatient case 10, a wavelength of 800 nm, and the right breast ofpatient 10; -
FIG. 20 g is a sectional image associated withpatient case 10, a wavelength of 850 nm, and the left breast ofpatient 10; and -
FIG. 20 h is a sectional image associated withpatient case 10, a wavelength of 850 nm, and the right breast ofpatient 10. - A system for scanning human tissue by diffuse-optical-spectroscopy includes: (a) source means for emitting electromagnetic radiation at one or more wavelengths corresponding to absorption associated with one or more human-tissue constituents, respectively; (b) receiving means for detecting received electromagnetic radiation at each of the one or more wavelengths; and (c) processing means for producing, in response to the received electromagnetic radiation, one or more cross-sectional images of the human tissue respectively associated with the one or more wavelengths.
- With reference to the Figures, a handheld diffuse optical breast scanning probe, an exemplary prototype of which is variously referred to herein as the probe and/or the DOB-
Scan probe 10, is operable for functional and compositional cross-sectional imaging of breast tissue 12 (FIG. 5 ). In order to evaluate performance and usability of the device, the DOB-Scan probe 10 is tested on breast tissue-mimicking phantoms 14 (FIGS. 6, 7 a, 7 b and 7 c) which have similar optical properties ofbreast tissue 12 and, thereafter, tested on human subjects. The DOB-Scan probe uses single-wavelength or multi-wavelengths light emitting diode(s) (LED), encapsulated light emitting diode(s) (eLED) 16, and/or laser(s) as an illumination source containing one or more visible, near infrared, and/or infrared wavelengths such as four near infrared wavelengths, 690 nm, 750 nm, 800 nm, and 850 nm, in one embodiment, LEDs of each eLED 16 are set 0.5 mm apart from each other (within the eLED 16) and perform as a multi-wavelengths pointed illumination source in such a scattering medium, A linear charge coupled device (CCD) 18, linear array photodiode(s), and/or a photodiode-array detector of the DOB-Scan probe 10 is used to measure the intensity of the scattered photons, in a line with 2048 points, on the surface of the breast 12 (or phantom 14) to create functional and cross-sectional images of thebreast tissue 12 underneath of the DOB-Scan probe 10. The illumination source wavelengths are chosen to correspond to wavelengths for which the breast fat has lowest absorption. - The DOB-
Scan probe 10 is used to create two-dimensional cross-sectional images ofbreast tissue 12 in-vivo. In the probe, an array detector (e.g. CCD 18) is used to create cross-sectional images ofbreast tissue 12. Thebreast tissue 12 is illuminated with twoeLEDs 16 which in an exemplary embodiment are located 60 mm away from each other (15 mm away from either side of the CCD 18) and are used to have symmetrical light illumination sources.FIGS. 1 and 2 present a sketch and photograph of the DOB-Scan probe 10, respectively. As it is illustrated inFIG. 1 andFIG. 2 , thelinear CCD 18 and light illumination sources (e.g. eLEDs 16) are located in reflectance geometry. The diffusion of light in the selected wavelengths are maximum due to minimum absorption of breast fat. - An exemplary embodiment of the DOB-
Scan probe 10 for functional and compositional cross-sectional imaging of thebreast tissue 12 employs multi-wavelength diffuse optical spectroscopy technique with four wavelengths encapsulated in aneLED 16 light source in the near infrared spectrum range. The device and associated system hardware provide four two-dimensional images of the breast tissue's function and composition. EacheLED 16 contains four wavelengths (e.g. 690 nm, 750 nm, 800 nm and 850 nm), which are illuminated into thebreast tissue 12 once a time and thelinear CCD 18 collects scattered light on the skin of thebreast tissue 12. Then, the system processes the data and transfers them to ahost computer 20 for further analysis and image reconstruction. From the collected data at one or more (e.g. four) different wavelengths, the system is able to create one or more cross-sectional optical absorption images for concentration of one or more different chromophores in the breast tissue such as Hb and HbO2 which are markers for cancerous tissue. Also, the prototypical probe and method accurately create cross-sectional optical absorption images such that there is no need to use laser diodes or fiber coupled laser diodes as an illumination source, although such illumination sources may be employed in variations of embodiments of the present invention. Real time data acquisition system components add functional imaging advantages to the probe. In the exemplary prototypical embodiment, the image reconstruction for each wavelength is performed at the rate of 24 Hz which is sufficient for functional imaging of biological activity. - A photograph of the DOB-
Scan probe 10 prototype with its system hardware housed within the DOB-Scan probe 10 is shown inFIG. 3 . In variations of embodiments, one or more system components may be housed externally to the DOB-Scan probe 10. Thehost computer 20 includes a processor and memory in a manner known in the art. WhileFIG. 3 shows awired connection 22 between the DOB-Scan probe 10 and thehost computer 20, in some embodiments the DOB-Scan probe 10 is battery-powered and operable to communicate with thehost computer 20 by any suitable wireless communications technology (e.g. Bluetooth™ or Wi-Fi). - Eight near infrared light sources are used to illuminate the
breast tissue 12 through two eLEDs 16. A Schematic diagram of the instrument is shown inFIG. 4 , including showing a power management unit andbattery charger 24, abattery 26, and awireless communication unit 28. Thewireless communication unit 28 may implement Bluetooth™, Wi-Fi and/or other wireless communications technology for example. Each wavelength is illuminated in thebreast tissue 12 once a time and thelinear CCD 18 collects scattered light on the skin of the breast tissue and converts it to analogue voltage. Then, analog signals are converted to digital signals and passed to the main processor. The microcontroller unit (MCU) 30 is responsible to create control signals for illumination sources (e.g. eLEDs 16), analog to digital convertor (ADC) 32 andCCD 18. TheMCU 30 reads digital data fromADC 32 and transfers data tohost computer 20 for further analysis, as well. - Two eLEDs 16 (e.g. from Marubeni America Corporation L690/750/800/850) are used in the
probe 10, which are located 15 mm away from each end-side of the detector (e.g. CCD 18) as shown inFIG. 1 . EachBLED 16 includes four wavelengths: 690 nm, 750 nm, 800 nm and 850 nm. - Penetrations of photons in these wavelengths are maximized due to low absorption of breast fat at the chosen wavelengths. In total, the probe has eight LEDs which can turn on and off individually or sequentially by the time period of 200 milliseconds. Intensity of each LED can be calibrated by trimmer potentiometer on the instrument (e.g. DOB-Scan probe 10).
- The light detector plays an important role in the instrument. In order to have high resolution images, it should have large number of light sensors with small size and patches. The light sources are located at two ends of the detector and the illuminated photons are travelling in different path lengths to reach to the photodetectors. The photodetectors which are located close to the active light source will be exposed to a higher light intensity and the ones that are placed far from active light source, will receive lower light intensity. Therefore, the photodetectors must have a large dynamic range to allow measurement for both small and large source-photodetector separations. The photodetector circuit of the prototype is specially designed to address these challenges. The light detector (ILX511, SONY™) used for the
probe 10 is a linear charge coupleddevice 18, which has a fixed capacitor associated with each photodetector (pixel). The photodetector chip of the prototype has 2086 pixels in total which is called one frame. 3.2 pixels at the beginning of the frame and 6 pixels at the end of the frame are dummy pixels. Therefore, the photodetector of the prototype has 2048 effective pixels with 14 μm pixel pitch; consequently the effective imaging area of the prototype is 28.672 mm (2048×14 μm). - NIR photons migration in biological tissue and optical properties of the breast tissue are compatible with diffusion optical spectroscopy. Four main absorbers in the breast tissue in NIR range (650 nm to 1100 nm) are deoxyhemoglobin (Hb), oxyhemoglobin (HbO2), water and fat. The optical properties of the breast tissue are widely studied and published in academic journal articles. These studies demonstrate that absorption of water and fat are low in NIR range, which allows photons to travel deeply into the breast tissue.
- The raw data acquired from the linear CCD 18 (2048 pixels, pixels pitch: 14 μm), two light sources and four NIR wavelengths are one frame which obtained from surface of the breast tissue. Therefore, total numbers of measurement points for one frame scan are 16384 (2048×2×4). The propagation of the light in a highly scattering medium like the
breast tissue 12 can be precisely modeled utilising the diffusion equation. As illustrated inFIG. 1 , source-detector separations in the DOB-Scan probe 10 vary from 15 mm to 43.672 rm. Therefore, illuminated light into the breast tissue pass different depth and path in the breast tissue to reach to the detectors. The measured photons travel longer path and deeper in thebreast tissue 12 while source-detector separation is wider. In order to calculate absorption coefficient (μa) of the paths photons travel in thebreast tissue 12, a closed form solution for the diffusion equation in a semi-infinite homogeneous medium may be employed while light source and detector are located in spatially reflectance geometry. The extracted absorption coefficients for photon paths are used to create two-dimensional cross-sectional image for each NIR wavelengths. - In particular, in a highly scattering medium such as
breast tissue 12, the radiative transfer equation can be simplified by the diffusion equation as follows: -
∇D(r)∇Φd(r)−μa c mΦd(r)=−S(r) (1) - where Φd represents the intensity at the detector located r mm away from the illumination source(S(r)). cm and μa are the photons velocity in the medium and absorption coefficient, respectively. D represents diffusion coefficient.
- Within the NIR spectrum range (650 nm to 1100 nm), the photons' migration in the biological tissue is in the scattering region and diffusion equation can be used to describe photons propagation. The following expression (2) presents analytical solution for the diffusion equation in a homogeneous media while the illumination source and detector setting in reflectance geometry.
-
- Where the parameters are defined as follows:
- μa Absorption coefficient
- μs Scattering coefficient
- μ′s Reduced scattering coefficient
- μeff Effective attenuation coefficient
- μ′t Total attenuation coefficient
- R(d) Measured scattered photon intensity at the distance of d from illumination source
- A Internal reflection parameter
- The diffusion equation is effective while r is greater than 10×(μa+μ′s)−1 and μa«μ′s.
- There are several studies demonstrating that there is not remarkable changes on the total attenuation coefficient (μ′t) of normal and cancerous breast tissue. Because, μa of the breast tissue is much smaller than so fluctuation in μa will not have remarkable variation on μ′t. These studies demonstrate that μa, and μ′s of the breast tissue are in the range of 0.002 mm−1 to 0.012 mm−1 and 0.6 mm−1 to 1.5 mm−1, respectively, at the NIR spectrum range. It has also been shown that variation on μ′t does not have notable results on the back scattered photons at the photodetector position.
- Oxy-hemoglobin (HbO2), deoxy-hemoglobin (Hb), water (H2O), and fat are the four primary chromophores in the breast tissue which contribute to the attenuation at wavelength λ and concentration of HbO2 and Hb vary by the time. The direct-approach-method equation (3) can be used to create cross-sectional images for the four chromophores concentration and concentration variations in Hb (ΔcHb) and HbO2 (ΔcHbO2) shown in the cross-sectional images.
-
- Where εn λ is the molar absorption extinction coefficient for the chromophore type n (n should be Hb, HbO2, H2O and Fat) at illuminated wavelength of λ (690 nm, 750 nm, 800 nm, 850 nm) and C, is the total concentration of the chromophore in the photons path lengths. While the exemplary wavelengths of 690, 750, 800 and 850 nm are mentioned herein in association with the chromophores of Hb, HbO2, H2O and Fat, it is understood that each of the wavelengths may vary in any range that is suitably associated with a corresponding chromophore or other human-tissue constituent or type.
- In the wider source-detector separation, back scattered light travels through deeper and longer paths in the tissue. Concentrations of Hb and HbO2 vary by the heartbeat. Concentration distribution of four absorbers can be calculated by solving equation (3) for different light paths to reconstruct images for the absorbers and visualize the variation of them in the breast tissue.
- The obtained raw data from the exemplary prototype having 2048 pixels, 2 sources and 4 wavelengths are measurement points collected from surface of the tissue. A reconstruction algorithm is useful for converting the surface measured light intensity into two-dimensional cross-sectional images of the chromophores. In the first step, the absorption coefficient (μa) of each light paths are calculated by utilizing equation (2) while the tissue is illuminated by each wavelength. In order to reduce effect of the noise on the measurements, we reduced the number of measurement points to 128 points (P1 to P128) by averaging 16 pixels. It can be assumed that the origin of the coordinate is located on the center of
light source # 1 as showed inFIG. 5 . As mentioned earlier, two sets of the data are collected for each wavelength: 1) whileeLED # 1 12 is on, 2) whileeLED # 2 12 is on. Assume we want to calculate the total absorption coefficient in location (X1, Y1) as shown inFIG. 5 . The illuminated light fromeLED # 1 12 travels along the path m and reaches to theCCD 14, and then intensity of scattered light is measured by the mth pixel of theCCD 14. A similar process occurs whileeLED # 2 12 is on. The illuminated light fromeLED # 2 12 travels along the path n and reaches to theCCD 14 and scattered light is measured by nth pixel of theCCD 14. Then the absorption coefficient at (X1, Y1) is calculated as a superposition of two light path lengths of illuminated light fromeLED # 1 12 andeLED # 2 12. - Table 1 presents a brief specification of the exemplary DOB-Scan probe in prototype form.
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TABLE 1 DOB-Scan Probe characteristics and parameter Parameter Value Wavelength 2 × (690 nm, 750 nm, 800 nm, 850 nm) Detector type Linear CCD Detector resolution 2048 pixels Image resolution 128 × 128 pixels Image length 28.67 mm Image depth 24 mm Detector sensitivity 1800 (V/Lx · S)@660 nm Illumination tech. Continuous wave Max. frame rate 24 Power consumption 100 mA @ 5 V
A MATLAB® (MATLAB R2012b, MathWorks®) based software interface gives the user complete controls of the hardware in addition to allowing them to collect, store, analyze data and create images, including color-mapped images, on the host computer. The software interface communicates with the DOB-Scan probe 10 via USB2.0 (or other serial communications technology) virtual comport and contains a graphical user interface (GUI) 34, as shown inFIG. 3 , for operator's ease of use. TheGUI 34 is comprised of a series of tabs and collected data graphs, which are used for various operations including: Manual and periodic light source selection,CCD 18 integration time setting and data collection. Upon launching the software interface the user must select illuminated wavelengths manually or set it periodically in LEDs control panel. In themanual mode 24 frames per second are collected from the image sensor, which it is sufficient for functional imaging of biological tissue. In periodic mode each wavelength is illuminated for 200 milliseconds and the data are collected with the same period. In this mode, eight frames are collected sequentially while different wavelengths are illuminated into the biological tissue, and in order to measure ambient light effect on the image sensor, one more frame is collected while all the LEDs are turned off. - To verify performance of the exemplary prototype of the designed DOB-
Scan probe 10, a study based upon tissue equivalent material was conducted. In this study, we used an incomplete (truncated) cylindrical shaped (8 cm diameter and 28 cm length) acetal resin rod 14 (Delrin®) and Intralipid® to mimicbackground breast tissue 12 due to similarity in optical properties to the breast fat. (μ′s=2.3 mm−1 and μa=0.002 nm−1). Also, optical properties of these two materials are well-documented and known. In order to mimic cancerous lesions, a 5mm hole 36 is placed on the side wall of thecylinder 14; located 15 mm below the incomplete side of theAcetal resin rod 14, as shown inFIG. 6 , which is filled with tumor-like liquid phantom. A solution ofIntralipid® 20% emulsion (Fresenius Kabi Inc), water and black ink (Higgins India ink) is used to mimic breast tumor in thebackground phantom 14. Black India ink is used in this study as an absorber for its flat absorption in the NIR spectrum range and it has water-solubility properties. - In the first experiment, we used
phantom # 1 14 in which thehole 36 was filled with tumor-like liquid phantom. The liquid-phantom is a solution consisting of 1 L water, 50 mL Intralipid® and 1.5 mL India black ink. In this case, we put theprobe 10 in three different locations on thephantom 14 in which the middle line of the probe 10 (middle of the CCD 18) is aligned: a) along the center of thehole 36, b) 7 mm to the left of thehole 36 center, and c) 7 mm to the right of thehole 36 center, as shown inFIGS. 7 a, 7 b, and 7 c , respectively. -
FIGS. 8 a, 8 b, and 8 c show the reconstructedcross-sectional images 38 of the phantom for the wavelength of 690 nm while the breast phantom'shole 36 contains the India black ink solution and while theprobe 10 is located: a) along the center of thehole 36, b) 7 mm to the left of thehole 36 center, and c) 7 mm to the right of thehole 36 center. Thereddish area 40 on the images shows the location of thehole 36 on the phantom, in contrast to differently colored areas such as the non-reddish area(s) 42. Since, black India ink has flat absorption spectrum in the NIR range, we have similar images for all wavelengths (690 nm, 750 nm, 800 nm and 850 nm). The test results demonstrate that theprobe 10 can capture any inhomogeneity in the phantom. - In the second experiment, we evaluated performance of the
probe 10 to accurately create a separate image for different absorbers such as Hb and HbO2. Therefore, we used protein assay reagent (PAR) to form inhomogeneity inside the phantom which has similar optical properties to HbO2 in the NIR spectrum range. We used a solution of 1.5 mL Pierce™ 660 nm PAR (Thermo Scientific™ Inc.) mixed with 10 μL Pre-diluted protein assay standards: Bovine Serum Albumin (BSA), with 1000 μg/mL in 0.9% saline and 0.05% sodium azide to make a PAR. The absorption coefficient of RBSA at 690 nm wavelength is 0.17 mm−1 which is much higher than its absorption coefficient at 750 nm, 800 nm and 850 nm (0,015 mm−1).FIGS. 9 a, 9 b, and 9 c showcross-sectional images 44 while theprobe 10 is placed on different locations of the phantom while the 690 nm light source is illuminated. - Since, absorption coefficient of the RBSA is much higher than surrounded material the
probe 10 can capture this difference.FIGS. 9 a, 9 b, and 9 c show reddish area(s) 40 corresponding to the locations (centrally aligned, to the left, and to the right) of the breast phantom'shole 36 containing the PAR, respectively, in contrast to non-reddish area(s) 42 that can be seen inFIGS. 9 a, 9 b, and 9 c . But for the 750 nm, 800 nm and 850 nm, theprobe 10 did not capture the inhomogeneity due to similar absorption coefficient of RBSA with surrounded material as demonstrated by theimages 46 shown inFIGS. 10 a, 10 b, and 10 c for the wavelength of 800 nm in which areddish area 40 is not visible. The three cross-sectional images ofFIGS. 10 a, 10 b, and 10 c produced by use of the DOB-Scan probe 10 show an absence ofreddish areas 40 corresponding to the location of the breast phantom's hole containing the PAR when the prototypicalhandheld probe 10 is emitting electromagnetic radiation at 800 nm and is located: a) along the center of thehole 36, b) to the left of thehole 36 center, and c) to the right of thehole 36 center. In contrast to the absence ofreddish areas 40, onlynon-reddish areas 42 are predominantly visible inFIGS. 10 a, 10 b , 10 c. - In order to evaluate the probe's effectiveness in monitoring the effect of therapy on the size of a tumor, we have done one more experiment in which the absorption coefficient of a cancerous-mimicking
phantom 14 is gradually increased by adding India black ink to a reference solution of water and Intralipid™ (50 ml/l), In this experiment, India black ink was added in 0.125 ml/l increments from 0.125 ml/l to 0.75 ml/l; however, the scattering coefficient of the medium was not affected by increasing the ink concentration. - Four images are reconstructed for each step. Therefore 24 images are created, in total. As mentioned before, we just present one image 48 (690 nm) for each step, because India black ink has a flat absorption spectrum in the NIR range, which is shown in
FIGS. 11 a to 11 f . That is.FIGS. 11 a to 11 f are six cross-sectional images produced by theprobe 10 showingreddish areas 40 corresponding to the location of the breast phantom's hole containing varying concentrations of India black ink when thehandheld probe 10 is emitting electromagnetic radiation at 690 nm. - The
reddish areas 40 and thenon-reddish areas 42 inimages 48 ofFIGS. 11 a to 11 f clearly show that the DOB-Scan probe 10 is capable of capturing any changes in the absorption coefficient of thetumor phantom 14. Therefore, the DOB-Scan probe 10 can be used to monitor the effect of the therapy on tumor size. - The performance of an exemplary prototype of the
probe 10 was evaluated by testing it on breast tissue-like phantom 14. The test results of the exemplary probe being used on breast tissue-like phantom show that theprobe 10 can create functional images and chromophores composition images of thephantom 14. - Extended data collected for cancerous tissue further demonstrates the probe's performance in its prototypical form, A technique to identify malignant breast tumors using contralateral healthy body tissue as a control is used to produce preliminary results for ten cases presented below.
- A clinical study was performed on ten patients who have been diagnosed with breast cancer. Before any measurements are taken, subjects' height, weight, age and gender were recorded. Details of subjects' breast cancer (for example, type of cancer, date of diagnosis, etc.) were also recorded. The subjects were asked to lie down on their back and then we placed the probe on the location of the breast that had cancer. The skin to which the probe is located is cleaned with alcohol prep pads. The same procedure has been done on the contralateral side of the breast. The test procedure takes approximately twenty minutes. We obtained preliminary data with DOB-Scan probe while that is located on the breast where diagnosed to have malignant tissue and its contralateral location on the healthy breast. Data gathered with cross sectional diffuse optical imaging modality processed and analysed to explore performance of the exemplary probes to detect and characterize malignant tissue in the breast, in-vivo.
- The reconstructed preliminary images show that the DOB-San probe can distinguish the cancerous region from the surrounding healthy tissue. The cancerous region is observable in the images (red area) due to a higher level of absorption caused by higher levels of vascularization. The red area on the images reflexes location of anomaly tissue in the breast.
- For each patient, two locations are scanned over cancerous lesion with the control as a contralateral healthy breast tissue. Four cross-sectional images are reconstructed for four NIR sources on each location. The test results below show that absorption coefficient of cancerous lesions are significantly higher than normal surrounding tissue. The main objective of this study was to validate the usability of developed DOB-Scan imaging set up to create cross-sectional images of the breast tissue and detect anomalies inside inhomogeneous breast tissue, based upon optical properties of the anomaly to the surrounding material.
- Table 2 represents brief clinical reports for the patients who enrolled in the further clinical study.
-
TABLE 2 Brief clinical reports for ten patients enrolled in the study. Patient Tumor Cancerous Diagnosis no. age position breast Tumor size Tumor type tools 1 53 10-12 left 2.5*0.8*0.8 Invasive Mammography o'clock lobular carcinoma 2 62 3-4 left 2.2 × 1.7 × solid Mammography o'clock 1.7 cm lobulated irregular mass 3 84 1 left 10 mm ductal Mammography o'clock carcinoma 4 79 9 right 7 mm Invasive Mammography, O'clock mammary MRI carcinoma 5 44 2 left 2.5 cm × 1.7 lobulated Mammography o'clock cm × 3.5 cm irregular mass 6 42 1 right 24 mm in Solid Mammography o'clock diameter mass with and US irregular margin 7 59 10-11 right 23*22*15 mm lobulated Mammography o'clock in diameter irregular mass 8 56 12-1 left 1.6 cm Lobulated Mammography o'clock in maximal hypoechoic and US dimension lesion 9 63 2 left 12 × 9 × Solid Mammography o'clock 9 mm Lesions and CBE 10 53 5 left 1.7 × 1.2 × Invasive Mammography o'clock 2.4 cm ductal and US carcinoma -
FIGS. 12 a to 12 h, 13 a to 13 h, 14 a to 14 h, 15 a to 15 h, 16 a to 16 h, 17 a to 17 h, 18 a to 18 h, 19 a to 19 h, and 20 a to 20 h represent reconstructedimages 50 for four different wavelengths while theprobe 10 positioned on the location where diagnosed having a cancerous lesion and its contralateral position for 9 of the 10 patients tabulated above. Thereddish area 40 on the images reflect the location of anomalous tissue, in contrast to thenon-reddish areas 42. - Referring to
FIGS. 12 a to 20 h , each breast has four images for four different wavelengths. Table 3 indicates the parameters associated with the Figures identified as “a” to “h” for each the Figure sets enumerated by thenumbers 12 to 20, For example,FIG. 12 a ,FIG. 13 a , and so on toFIG. 20 a are each associated with the wavelength 690 nm and the left breast, whileFIG. 12 b ,FIG. 13 b , and so on toFIG. 20 h are each associated with 690 nm and the right breast. -
TABLE 3 Test parameters associated with FIGS. 12a to 20h FIG. Identification Wavelength Breast a 690 nm left b 690 nm right c 750 nm left d 750 nm right e 800 nm left f 800 nm right g 850 nm left h 850 nm right
Each of the ten cases are described below. - Case 1: There was two cancerous lesions in the left breast and the
probe 10 located on the one located at 10 o'clock of the left breast.FIGS. 12 a to 12 h present that theprobe 10 captured the tumor correctly. There are somereddish areas 40 on the healthy breast (FIGS. 12 b, 12 d, 12 f, and 12 h ) which caused by saturation of the detector. - Case 2: There was two cancerous lesions in the left breast and the
probe 10 located on the one located at 4 o'clock of the left breast.FIGS. 13 a to 13 h present that theprobe 10 captured the tumor correctly and it is in line with mammography images. There are somereddish areas 40 on the healthy breast (FIGS. 13 b, 13 d, 13 f, and 13 h ) which caused by saturation of the detector. - Case 3: There was a cancerous lesions in the left breast at 1 o'clock.
FIGS. 14 a to 14 h presents that theprobe 10 captured the tumor correctly and it is in line with mammography images. There are somereddish areas 40 on the healthy breast (FIGS. 14 b, 14 d, 14 f, and 14 h ) which caused by saturation of the detector. - Case 4: There was a cancerous lesions in the right breast at 9 o'clock. This patient had large breasts with a cancerous lesion positioned near to the chest wall. In these cases, the
probe 10 captures the cancerous lesion with difficulty due to the depth of the tumor in the breast (FIGS. 15 b, 15 d, 15 f , and 15 h). There are somereddish areas 40 on the healthy breast (FIGS. 15 a, 15 c, 15 e, and 15 g ) which caused by saturation of the detector. - Case 5: There was a cancerous lesions in the left breast at 2 o'clock. Reconstructed images for the left breast of patient #5 (
FIGS. 16 a, 16 c, 16 e , and 16 g) show that the absorption coefficient of the whole breast is unusually higher than for the malignant tissue. Medical advice from the patient's oncologists indicates there was a lobulated irregular large shadowing mass which had spread all over the left breast. For this reason, theprobe 10 shows higher absorption coefficient whole over the breast. There are somereddish areas 40 on the healthy breast (FIGS. 16 b, 16 d, 16 f, and 16 h ) which caused by saturation of the detector. - Case 6: There was a cancerous lesions in the right breast at 1 o'clock.
FIGS. 17 a to 17 h present that theprobe 10 captured the tumor correctly and it is in line with mammography images. There are somereddish areas 40 on the healthy breast which shows a mass in healthy tissue that has a higher absorption coefficient at 800 nm (FIG. 17 e ) and 850 nm (FIG. 17 g ). According toFIGS. 17 a to 17 h , water and fat have higher absorption coefficients than the other two absorbers in breast tissue. Medical advice from the patients oncologists indicates there was a benign mass which is filled with liquid at the location. - Case 7: There was a cancerous lesions in the right breast between 10 and 11 o'clock.
FIGS. 18 a to 18 h present that theprobe 10 captured the tumor correctly and it is in line with mammography images. The DOB-Scan probe's images also show a mass in healthy tissue (FIGS. 18 a . 18 c, 18 e, and 18 g) that has a higher absorption coefficient. Medical advice from the patient's oncologists indicates there was a benign mass which filled with liquid at the location. - Case 8: Data collection failed due to technical problem. Thus, images corresponding to this case are not provided in association with the present invention.
- Case 9: There was a cancerous lesions in the left breast at 1 o'clock.
FIGS. 19 a to 19 h present that theprobe 10 captured the tumor correctly and it is in line with mammography images. - Case 10: There was a cancerous lesions in the left breast at 5 o'clock.
FIGS. 20 a to 20 h present that theprobe 10 captured the tumor correctly and it is in line with mammography images. - As shown, the DOB-
Scan probe 10 can be used to capture cross-sectional images of the female breast's interior using NIR light sources associated with a linear CCD. The reconstructed preliminary images show that DOB-San probe can identify the cancerous region from the surrounding healthy tissue. The cancerous region is observed in the images due to the higher level of absorption caused by higher levels of vascularization in the cancerous region. - Thus, there is provided a diffuse-optical-spectroscopy system for scanning human tissue. The system includes: (a) a handheld probe operable to emit electromagnetic radiation at one or more wavelengths corresponding to absorption associated with one or more human-tissue constituents, respectively, the handheld probe being operable to detect received electromagnetic radiation at each of the one or more wavelengths: and (b) a processor operable to produce, in response to the received electromagnetic radiation, one or more cross-sectional images of the human tissue respectively associated with the one or more wavelengths.
- While embodiments of the invention have been described and illustrated, such embodiments should be considered illustrative of the invention only. The invention may include variants not described or illustrated herein in detail. Thus, the embodiments described and illustrated herein should not be considered to limit the invention as construed in accordance with the accompanying claims.
Claims (21)
1-16. (canceled)
17. An-optical-spectroscopy system for scanning human tissue, the system comprising:
a handheld probe having:
at least one source configured to emit electromagnetic radiation at two or more wavelengths respectively corresponding to absorption associated with one or more human-tissue constituents, respectively, the handheld probe configured to detect reflected electromagnetic radiation at each of the two or more wavelengths; and
at least one detector configured to detect emitted electromagnetic radiation reflected from the one or more human-tissue constituents; and
a processor, coupled to the handheld probe, configured to produce, in response to the received electromagnetic radiation, one or more cross-sectional images of the human tissue respectively associated with one of the two or more wavelengths.
18. The system of claim 17 , wherein the handheld probe comprises a sensor for detecting received electromagnetic radiation, wherein the sensor is aligned along a first axis and facing in an outward direction.
19. The system of claim 17 wherein the handheld probe comprises two sources for emitting the electromagnetic radiation, the sources being aligned along the first axis, facing in the outward direction, and disposed equidistant from the sensor.
20. The system of claim 17 wherein the handheld probe comprises three sources for emitting the electromagnetic radiation, each of the three sources facing in the outward direction and disposed about the sensor.
21. The system of claim 17 wherein the one or more wavelengths comprise at least four wavelengths corresponding to absorption associated with at least four human-tissue constituents, each of the at least four wavelengths being in at least one of a visible region, a near-infrared region, and an infrared region of the electromagnetic spectrum, said at least four human-tissue constituents comprising deoxyhemoglobin, oxyhemoglobin, water and fat.
22. The system of claim 17 wherein the source comprises first, second, third and fourth light sources configured to emit electromagnetic radiation at least at first, second, third and fourth wavelengths, respectively, wherein each of the at least first, second, third and fourth light sources comprising a light source selected from the group consisting of a light-emitting diode, an encapsulated light-emitting diode, and a laser.
23. The system of claim 17 wherein the handheld probe comprises a detector selected from the group consisting of a charge-coupled device and an array photodiode.
24. The system of claim 17 wherein the one or more wavelengths further comprises wavelengths from the group comprising 600 nm to 1100 nm.
25. The system of claim 17 , further comprising at least a second detector configured to detect emitted electromagnetic radiation reflected from the one or more human-tissue constituents.
26. The system of claim 17 , further comprising a sensor to detect a change in orientation of the axis with respect to the reflected electromagnetic radiation in relation to the one or more human-tissue constituents.
27. The system of claim 17 , further comprising a sensor to detect pressure between the handheld probe and the at least one human-tissue constituent.
28. A method of scanning human tissue by diffuse-optical-spectroscopy, the method comprising:
emitting electromagnetic radiation by at least one emitter source at one or more wavelengths corresponding to absorption associated with one or more human-tissue constituents, respectively, by a handheld probe while being placed in proximity to the human tissue at an axis or orientation;
detecting by a detector housed in the handheld probe received electromagnetic radiation at each of the one or more wavelengths; and
in response to the received electromagnetic radiation, executing computer-readable instructions at a processor to generate one or more cross-sectional images of the human tissue respectively associated with the one or more wavelengths.
29. The method of claim 28 wherein the detecting further comprises detecting the received electromagnetic radiation by one or more sensors of said handheld probe when said one or more sensors are aligned along a first axis and facing in an outward direction.
30. The method of claim 28 wherein the emitting further comprises emitting electromagnetic radiation by first and second emitter sources when said first and second emitter sources are aligned along said first axis, facing in said outward direction, and disposed on either side of the detector.
31. The method of claim 28 the emitting further comprises emitting the electromagnetic radiation at said one or more wavelengths comprising at least four wavelengths corresponding to absorption associated with at least four human-tissue constituents, each of said at least four wavelengths being in at least one of a visible region, a near-infrared region, and an infrared region of the electromagnetic spectrum, said at least four human-tissue constituents comprising deoxyhemoglobin, oxyhemoglobin, water and fat.
32. The method of claim 28 the emitting comprises emitting said electromagnetic radiation by first, second, third and fourth emitter sources at first, second, third and fourth wavelengths, respectively, of the one or more wavelengths when each of the first, second, third and fourth light sources are selected from the group consisting of a light-emitting diode, an encapsulated light-emitting diode, and a laser.
33. The method of claim 28 the detecting further comprises detecting received electromagnetic radiation by at least one of a charge-coupled device and a photodiode array.
34. The method of claim 28 the emitting further comprises emitting continuous wave electromagnetic radiation.
35. The method of claim 28 further comprising:
determining a first absorption coefficient associated with a first emitter source when the first emitter source is emitting first electromagnetic radiation,
determining a second absorption coefficient associated with a second emitter source when the second emitter source is emitting second electromagnetic radiation; and
determining a total absorption coefficient at an intersecting location within the human tissue by calculating a superposition value associated with the first absorption coefficient and the second absorption coefficient.
36. The method of claim 28 further comprising:
detecting a pressure between the handheld probe and the one or more human-tissue constituents; and
detecting an orientation with respect to the axis between the handheld probe and the one or more human-tissue constituents.
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US201916479025A | 2019-07-18 | 2019-07-18 | |
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