US20220356423A1 - Method for improving aftertaste of alcoholic beverages using allulose - Google Patents

Method for improving aftertaste of alcoholic beverages using allulose Download PDF

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US20220356423A1
US20220356423A1 US17/870,417 US202217870417A US2022356423A1 US 20220356423 A1 US20220356423 A1 US 20220356423A1 US 202217870417 A US202217870417 A US 202217870417A US 2022356423 A1 US2022356423 A1 US 2022356423A1
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vol
alcohol
allulose
sensory
alcoholic beverage
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US17/870,417
Inventor
Jong Min Choi
Su-Jeong KIM
Youn-Kyung BAK
Jung Gyu Park
Sung Bae Byun
Dong Seok SHIM
In Lee
Seung Won Park
Dong Chul JUNG
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CJ CheilJedang Corp
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CJ CheilJedang Corp
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Priority to US17/870,417 priority Critical patent/US20220356423A1/en
Publication of US20220356423A1 publication Critical patent/US20220356423A1/en
Priority to US18/436,536 priority patent/US20240174956A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G3/00—Preparation of other alcoholic beverages
    • C12G3/08—Preparation of other alcoholic beverages by methods for altering the composition of fermented solutions or alcoholic beverages not provided for in groups C12G3/02 - C12G3/07
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G3/00—Preparation of other alcoholic beverages
    • C12G3/04—Preparation of other alcoholic beverages by mixing, e.g. for preparation of liqueurs
    • C12G3/06—Preparation of other alcoholic beverages by mixing, e.g. for preparation of liqueurs with flavouring ingredients
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/88—Taste or flavour enhancing agents
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12H—PASTEURISATION, STERILISATION, PRESERVATION, PURIFICATION, CLARIFICATION OR AGEING OF ALCOHOLIC BEVERAGES; METHODS FOR ALTERING THE ALCOHOL CONTENT OF FERMENTED SOLUTIONS OR ALCOHOLIC BEVERAGES
    • C12H6/00—Methods for increasing the alcohol content of fermented solutions or alcoholic beverages
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12H—PASTEURISATION, STERILISATION, PRESERVATION, PURIFICATION, CLARIFICATION OR AGEING OF ALCOHOLIC BEVERAGES; METHODS FOR ALTERING THE ALCOHOL CONTENT OF FERMENTED SOLUTIONS OR ALCOHOLIC BEVERAGES
    • C12H6/00—Methods for increasing the alcohol content of fermented solutions or alcoholic beverages
    • C12H6/02—Methods for increasing the alcohol content of fermented solutions or alcoholic beverages by distillation

Definitions

  • the present invention relates to an agent for improving an alcohol flavor of an alcoholic beverage, which includes allulose-containing saccharides, and a method of improving an alcohol flavor of an alcoholic beverage using allulose.
  • Ethanol i.e., ethyl alcohol
  • Ethanol contained in foods has an unpleasant flavor such as unique alcohol smell or bitterness.
  • Such an unpleasant flavor becomes stronger with increasing alcohol concentration.
  • soju or rum increase in sales is slowing down due to the unpleasant flavor thereof.
  • soju with low alcohol content or fruit soju with added flavor and increase in sales volume represents changes in preference of consumers who avoid the strong bitterness and smell of alcohol.
  • glycoside-based sweetener such as steviol glycoside or enzyme-treated stevia instead of a sugar-based sweetener such as sugar or fructose in order to reduce the flavor of soju and to mask a bitterness and tangy taste thereof
  • a glycoside-based sweetener such as steviol glycoside or enzyme-treated stevia
  • sugar-based sweetener such as sugar or fructose
  • the present inventors have made efforts to reduce smell and bitterness of alcohol of alcoholic foods. As a result, the present inventors found that addition of allulose can mask the smell and bitterness of alcohol for a given amount of alcohol, and thus completed the present invention.
  • an agent for improving an alcohol flavor of an alcoholic beverage includes allulose-containing saccharides and ethanol.
  • the allulose according to the present invention may be extracted directly from natural products, or may be chemically or biologically synthesized, without being limited thereto.
  • the allulose may be provided in crystal form or in allulose-containing syrup form (i.e., in liquid form).
  • alcoholic beverage refers to a beverage containing an edible alcohol (i.e., ethanol).
  • the ethanol may be present in an amount of 6% by volume (vol %) to 70 vol %, specifically 6 vol % to 50 vol %, 6 vol % to 45 vol %, 6 vol % to 40 vol %, 6 vol % to 30 vol %, 6 vol % to 25 vol %, 6 vol % to 20 vol %, 6 vol % to 18 vol %, 8 vol % to 70 vol %, 8 vol % to 50 vol %, 8 vol % to 45 vol %, 8 vol % to 40 vol %, 8 vol % to 30 vol %, 8 vol % to 25 vol %, 8 vol % to 20 vol %, 8 vol % to 18 vol %, 13 vol % to 70 vol %, 13 vol % to 50 vol %, 13 vol % to 45 vol %, 13 vol % to 40 vol %, 13 vol % to 30 vol %, 13 vol % to 25 vol %, 13 vol % to 20 vol %, 13 vol % to 18 vol %
  • the alcoholic beverage may be a distilled alcoholic beverage or a fermented alcoholic beverage.
  • distilled alcoholic beverage refers to an alcoholic beverage obtained by distilling alcohol-fermented fruit juices, plant juices, or brewed starches (cereals).
  • the alcoholic beverage may be a distilled alcoholic beverage, more specifically Soju, kaoliang liquor, brandy, Armagnac, whiskey, vodka, rum, gin, tequila or cognac.
  • the alcoholic beverage may be a fermented alcoholic beverage, more specifically beer, wine, sake, or makgeolli.
  • the alcohol flavor may be an alcohol bitterness and/or an alcohol smell.
  • improving the alcohol flavor may include reducing or masking the alcohol flavor.
  • the allulose may be present in an amount of 50 parts by weight to 100 parts by weight, specifically 70 parts by weight to 100 parts by weight, 90 parts by weight to 100 parts by weight, 95 parts by weight to 100 parts by weight, 98 parts by weight to 100 parts by weight, 98.5 parts by weight to 100 parts by weight, 99 parts by weight to 100 parts by weight, or 99.5 parts by weight to 100 parts by weight, relative to 100 parts by weight of the saccharides in terms of dried solid content.
  • the saccharides may further include at least one sweetener, in addition to allulose.
  • the sweetener may include any known sweetener, for example, monosaccharides, disaccharides, oligosaccharides, sugar alcohols, and high-strength sweeteners, without being limited thereto.
  • the monosaccharides may include arabinose, xylose, fructose, tagatose, allose, and galactose.
  • the disaccharides refer to a group of carbohydrates consisting of two monosaccharide units linked together, and examples thereof may include lactose, maltose, trehalose, turanose, and cellobiose.
  • the oligosaccharides refer to a group of carbohydrates consisting of 3 or more monosaccharide units linked together, and examples thereof may include fructooligosaccharide, isomaltooligosaccharide, xylooligosaccharide, gentio-oligosaccharide, maltooligosaccharide, and galactooligosaccharide.
  • the sugar alcohols refer to compounds obtained by reducing a carbonyl group in saccharides, and examples thereof may include erythritol, xylitol, arabitol, mannitol, sorbitol, maltitol, and lactitol.
  • the high-strength sweeteners refers to sweeteners having a sweetness ten times or greater that of sugar, and examples thereof may include aspartame, acesulfame K, rebaudioside A, and sucralose, without being limited thereto.
  • the saccharides according to the present invention may be free from sucrose, glucose, or a combination thereof.
  • the allulose-containing saccharides may be present in an amount of 0.1 g to 10 g, specifically 0.1 g to 3.0 g or 0.3 g to 3.0 g per 1 L of the alcoholic beverage.
  • the allulose may be present in an amount of 5 g to 600 g, specifically 5 g to 170 g, 16 g to 170 g, 5.55 g to 166.7 g, or 16.7 g to 166.7 g per 1 L of the ethanol.
  • the ethanol as a reference refers to pure ethanol (i.e., 100% ethanol).
  • a method of improving an alcohol flavor of an alcoholic beverage includes: adding allulose to the alcoholic beverage; or adding allulose to a beverage, followed by adding ethanol to the beverage.
  • the alcoholic beverage may be prepared commercially by distilling a mash obtained through a process in which cereal grains or potatoes, as raw materials, are subjected to alcohol fermentation, followed by aging, or by diluting water-free alcohol with water and adding a sweetener and the like to the diluted alcohol.
  • An allulose-containing alcoholic beverage may be prepared by adding the allulose to the prepared alcoholic beverage, or by adding the allulose to the raw materials in preparation of the alcoholic beverage.
  • the allulose, the ethanol, the alcoholic beverage, the saccharides, the alcohol flavor, and improving alcohol flavor are the same as described in the above aspect.
  • the present invention provides a method in which allulose is added to alcoholic foods having a high alcohol content, especially alcoholic beverages to mask unpleasant alcohol bitterness and smell, thereby considerably improving sensory preference of the foods without using separate additives.
  • FIG. 1 is a graph showing a relationship between alcohol concentration and alcohol bitterness according to Examples and Comparative Examples.
  • FIG. 2 is a graph showing a relationship between alcohol concentration and alcohol smell according to Examples and Comparative Examples.
  • alcoholic beverages have varying alcohol contents depending upon the kind and manufacturing method thereof.
  • Beer has an alcohol content of 4 vol % to 12 vol % (generally 5 vol %)
  • wine has an alcohol content of 6 vol % to 12 vol % (generally 8 vol %)
  • Sake has an alcohol content of 8 vol % to 15 vol % (generally 13 vol %)
  • soju has an alcohol content of 14 vol % to 20 vol % (generally 18 vol %).
  • diluted soju having an alcohol content of 8.0% or more was prepared to check change in quality due to addition of allulose.
  • Diluted soju samples of Comparative Examples 1 to 3 and Examples 1 to 3 were prepared as follows. First, purified water was added to a rectified spirit having an alcohol concentration of 95 vol %, thereby preparing 10 L of a primarily diluted rectified spirit having an alcohol concentration of 50 vol %. Then, activated carbon powder was mixed with the primarily diluted rectified spirit, followed by aging and deodorization for 1 to 3 hours, and then the resulting mixture was clarified using a filter containing diatomaceous earth (SELECT® A20, BEGEROW Co.).
  • sugar purity: 98 wt % or more, White Sugar, CJ Cheiljedang
  • allulose crystal allulose, purity: 98 wt % or more, CJ Cheiljedang
  • the secondarily diluted rectified spirit was clarified in the same manner as the primarily diluted rectified spirit.
  • Sensory evaluation was carried out by 15 trained panelists.
  • the panelists were provided with the standard products as Sensory Comparison Examples (Table 2) and trained to be fully aware of an unpleasant bitterness and smell inherent to alcohol and compare intensity of the bitterness and smell to match a randomly offered diluted soju sample to the alcohol concentration thereof.
  • sensory evaluation was carried out through the following procedure. First, the panelists were placed in separate rooms, respectively, and then provided with Sensory Comparison Examples to be aware of the bitterness and smell inherent to alcohol, once more, prior to being provided with the samples of Examples and Comparative Examples. Thereafter, the samples of Example 1 and Comparative Example 1, each labelled with a three-digit random number, were provided in a transparent cup one by one in a double-blind fashion. During evaluation, each of the panelists was instructed to hold the sample in his/her mouth to taste the sample before spitting the sample out, to be prevented from becoming intoxicated due to excessive intake. In addition, after evaluation of each sample, the panelists were allowed to rinse their mouths with cooked rice and water to prepare for evaluation of the next sample. Each of the trained panelists evaluated intensity of the smell and bitterness of alcohol of each of the samples of Examples and Comparative Examples on a 9-point scale (as shown in Table 2) with reference to Sensory Comparison Examples.
  • Example 1 and Comparative Example 1 For evaluation of Example 1 and Comparative Example 1, evaluation of Example 2 and Comparative Example 2, and evaluation of Example 3 and Comparative Example 3, Sensory Comparison Examples A1 to A5, Sensory Comparison Examples B1 to B5, and Sensory Comparison Examples C1 to C5 were provided, respectively. In addition, after each evaluation, the panelists were allowed to rest for at least 12 to 24 hours to avoid confusion due to excessive intake.
  • bitterness of alcohol decreased due to increase in sweetness with increasing concentration of sugar or allulose.
  • sweetness intensity of allulose is about 70% that of sugar, it can be seen that reduction effects in smell and bitterness of alcohol is not due to simply sweetness enhancement by addition of saccharides but due to efficacy of allulose.

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  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
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  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Nutrition Science (AREA)
  • Alcoholic Beverages (AREA)
  • Distillation Of Fermentation Liquor, Processing Of Alcohols, Vinegar And Beer (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Seasonings (AREA)
  • Preparation Of Fruits And Vegetables (AREA)
  • Non-Alcoholic Beverages (AREA)

Abstract

The present invention relates to an agent for improving aftertaste of alcoholic beverages comprising allulose-containing saccharides, and a method for improving aftertaste of alcoholic beverages using allulose.

Description

    RELATED APPLICATION
  • The present application is a continuation application of U.S. application Ser. No. 16/336,604, filed Mar. 26, 2019, which is a 371 application of PCT/KR2017/010847, filed Sep. 28, 2017, which claims priority to Korean Application No. 10-2016-0130094, filed Oct. 7, 2016. U.S. application Ser. No. 16/336,604 is herein incorporated by reference in its entirety.
    • FIELD OF THE INVENTION
  • The present invention relates to an agent for improving an alcohol flavor of an alcoholic beverage, which includes allulose-containing saccharides, and a method of improving an alcohol flavor of an alcoholic beverage using allulose.
    • DESCRIPTION OF THE RELATED ART
  • Ethanol (i.e., ethyl alcohol) contained in foods has an unpleasant flavor such as unique alcohol smell or bitterness. Such an unpleasant flavor becomes stronger with increasing alcohol concentration. Particularly, in the case of distilled alcoholic beverage having a high alcohol concentration, such as soju or rum, increase in sales is slowing down due to the unpleasant flavor thereof. Recent development of soju with low alcohol content or fruit soju with added flavor and increase in sales volume represents changes in preference of consumers who avoid the strong bitterness and smell of alcohol.
  • There has been proposed a method of using a glycoside-based sweetener such as steviol glycoside or enzyme-treated stevia instead of a sugar-based sweetener such as sugar or fructose in order to reduce the flavor of soju and to mask a bitterness and tangy taste thereof (Korean Patent No. 10-1320082). However, such a glycoside-based sweetener has poor sensory properties such as delayed sweetness, unique bitterness, or foreign body sensation.
  • In addition, there has been proposed a method of applying allulose to beer or the like (Japanese Patent No. 5764308). However, in this method, allulose is used to maintain or improve a flavor of raw materials, and use of allulose to suppress a smell and bitterness unique to alcohols has not been proposed yet.
  • The present inventors have made efforts to reduce smell and bitterness of alcohol of alcoholic foods. As a result, the present inventors found that addition of allulose can mask the smell and bitterness of alcohol for a given amount of alcohol, and thus completed the present invention.
    • SUMMARY OF THE INVENTION
  • It is one object of the present invention to provide an agent for improving an alcohol flavor of alcoholic foods, which includes allulose-containing saccharides.
  • It is another object of the present invention to provide a method of improving an alcohol flavor of alcoholic foods using allulose.
  • In accordance with one aspect of the present invention, an agent for improving an alcohol flavor of an alcoholic beverage includes allulose-containing saccharides and ethanol.
  • The allulose according to the present invention may be extracted directly from natural products, or may be chemically or biologically synthesized, without being limited thereto. In addition, the allulose may be provided in crystal form or in allulose-containing syrup form (i.e., in liquid form).
  • As used herein, “alcoholic beverage” refers to a beverage containing an edible alcohol (i.e., ethanol).
  • In one embodiment, the ethanol may be present in an amount of 6% by volume (vol %) to 70 vol %, specifically 6 vol % to 50 vol %, 6 vol % to 45 vol %, 6 vol % to 40 vol %, 6 vol % to 30 vol %, 6 vol % to 25 vol %, 6 vol % to 20 vol %, 6 vol % to 18 vol %, 8 vol % to 70 vol %, 8 vol % to 50 vol %, 8 vol % to 45 vol %, 8 vol % to 40 vol %, 8 vol % to 30 vol %, 8 vol % to 25 vol %, 8 vol % to 20 vol %, 8 vol % to 18 vol %, 13 vol % to 70 vol %, 13 vol % to 50 vol %, 13 vol % to 45 vol %, 13 vol % to 40 vol %, 13 vol % to 30 vol %, 13 vol % to 25 vol %, 13 vol % to 20 vol %, 13 vol % to 18 vol %, 15 vol % to 70 vol %, 15 vol % to 50 vol %, 15 vol % to 45 vol %, 15 vol % to 40 vol %, 15 vol % to 30 vol %, 15 vol % to 25 vol %, 15 vol % to 20 vol %, or 15 vol % to 18 vol %, based on the total volume of the alcoholic beverage.
  • In one embodiment, the alcoholic beverage may be a distilled alcoholic beverage or a fermented alcoholic beverage. As used herein, “distilled alcoholic beverage” refers to an alcoholic beverage obtained by distilling alcohol-fermented fruit juices, plant juices, or brewed starches (cereals).
  • Specifically, the alcoholic beverage may be a distilled alcoholic beverage, more specifically Soju, kaoliang liquor, brandy, Armagnac, whiskey, vodka, rum, gin, tequila or cognac.
  • In addition, the alcoholic beverage may be a fermented alcoholic beverage, more specifically beer, wine, sake, or makgeolli.
  • In one embodiment, the alcohol flavor may be an alcohol bitterness and/or an alcohol smell.
  • In one embodiment, improving the alcohol flavor may include reducing or masking the alcohol flavor.
  • In one embodiment, the allulose may be present in an amount of 50 parts by weight to 100 parts by weight, specifically 70 parts by weight to 100 parts by weight, 90 parts by weight to 100 parts by weight, 95 parts by weight to 100 parts by weight, 98 parts by weight to 100 parts by weight, 98.5 parts by weight to 100 parts by weight, 99 parts by weight to 100 parts by weight, or 99.5 parts by weight to 100 parts by weight, relative to 100 parts by weight of the saccharides in terms of dried solid content.
  • The saccharides may further include at least one sweetener, in addition to allulose. Examples of the sweetener may include any known sweetener, for example, monosaccharides, disaccharides, oligosaccharides, sugar alcohols, and high-strength sweeteners, without being limited thereto. Examples of the monosaccharides may include arabinose, xylose, fructose, tagatose, allose, and galactose. The disaccharides refer to a group of carbohydrates consisting of two monosaccharide units linked together, and examples thereof may include lactose, maltose, trehalose, turanose, and cellobiose. The oligosaccharides refer to a group of carbohydrates consisting of 3 or more monosaccharide units linked together, and examples thereof may include fructooligosaccharide, isomaltooligosaccharide, xylooligosaccharide, gentio-oligosaccharide, maltooligosaccharide, and galactooligosaccharide. The sugar alcohols refer to compounds obtained by reducing a carbonyl group in saccharides, and examples thereof may include erythritol, xylitol, arabitol, mannitol, sorbitol, maltitol, and lactitol. The high-strength sweeteners refers to sweeteners having a sweetness ten times or greater that of sugar, and examples thereof may include aspartame, acesulfame K, rebaudioside A, and sucralose, without being limited thereto. In another embodiment, the saccharides according to the present invention may be free from sucrose, glucose, or a combination thereof.
  • In one embodiment, the allulose-containing saccharides may be present in an amount of 0.1 g to 10 g, specifically 0.1 g to 3.0 g or 0.3 g to 3.0 g per 1 L of the alcoholic beverage.
  • In one embodiment, the allulose may be present in an amount of 5 g to 600 g, specifically 5 g to 170 g, 16 g to 170 g, 5.55 g to 166.7 g, or 16.7 g to 166.7 g per 1 L of the ethanol. Herein, the ethanol as a reference refers to pure ethanol (i.e., 100% ethanol).
  • In accordance with another aspect of the present invention, a method of improving an alcohol flavor of an alcoholic beverage includes: adding allulose to the alcoholic beverage; or adding allulose to a beverage, followed by adding ethanol to the beverage.
  • The alcoholic beverage may be prepared commercially by distilling a mash obtained through a process in which cereal grains or potatoes, as raw materials, are subjected to alcohol fermentation, followed by aging, or by diluting water-free alcohol with water and adding a sweetener and the like to the diluted alcohol. An allulose-containing alcoholic beverage may be prepared by adding the allulose to the prepared alcoholic beverage, or by adding the allulose to the raw materials in preparation of the alcoholic beverage.
  • In the method of improving an alcohol flavor of an alcoholic beverage, the allulose, the ethanol, the alcoholic beverage, the saccharides, the alcohol flavor, and improving alcohol flavor are the same as described in the above aspect.
  • The present invention provides a method in which allulose is added to alcoholic foods having a high alcohol content, especially alcoholic beverages to mask unpleasant alcohol bitterness and smell, thereby considerably improving sensory preference of the foods without using separate additives.
    • BRIEF DESCRIPTION OF DRAWINGS
  • FIG. 1 is a graph showing a relationship between alcohol concentration and alcohol bitterness according to Examples and Comparative Examples.
  • FIG. 2 is a graph showing a relationship between alcohol concentration and alcohol smell according to Examples and Comparative Examples.
    •
  • (※ In the drawings, * indicates a statistically significant trend (p<0.1), and ** indicates a statistically significant difference (p<0.05).)
    • DETAILED DESCRIPTION OF THE INVENTION
  • Hereinafter, the present invention will be described in more detail with reference to examples. However, it should be noted that these examples are provided for illustration only and should not be construed in any way as limiting the invention. In addition, these examples are provided for more complete understanding of the present invention by one of ordinary skill in the art.
    • EXPERIMENTAL EXAMPLE 1 Preparation and Quality Analysis of Diluted Soju
  • Generally, alcoholic beverages have varying alcohol contents depending upon the kind and manufacturing method thereof. Beer has an alcohol content of 4 vol % to 12 vol % (generally 5 vol %), wine has an alcohol content of 6 vol % to 12 vol % (generally 8 vol %), Sake has an alcohol content of 8 vol % to 15 vol % (generally 13 vol %), and soju has an alcohol content of 14 vol % to 20 vol % (generally 18 vol %). For an alcoholic beverage having an alcohol content of less than 6 wt %, since an unpleasant flavor of alcohol is not significant, diluted soju having an alcohol content of 8.0% or more was prepared to check change in quality due to addition of allulose.
  • (1) Preparation of Diluted Soju
  • Diluted soju samples of Comparative Examples 1 to 3 and Examples 1 to 3 were prepared as follows. First, purified water was added to a rectified spirit having an alcohol concentration of 95 vol %, thereby preparing 10 L of a primarily diluted rectified spirit having an alcohol concentration of 50 vol %. Then, activated carbon powder was mixed with the primarily diluted rectified spirit, followed by aging and deodorization for 1 to 3 hours, and then the resulting mixture was clarified using a filter containing diatomaceous earth (SELECT® A20, BEGEROW Co.). Then, sugar (purity: 98 wt % or more, White Sugar, CJ Cheiljedang) or allulose (crystal allulose, purity: 98 wt % or more, CJ Cheiljedang) was mixed with the clarified mixture in amounts as listed in Table 1, followed by adding purified water, thereby preparing 1 L of a secondarily diluted rectified spirit. Then, the secondarily diluted rectified spirit was clarified in the same manner as the primarily diluted rectified spirit.
  • In addition, purified water was added to the clarified primarily diluted rectified spirit to dilute the spirit to alcohol concentrations as listed in Table 2, thereby preparing Sensory Comparison Examples for sensory evaluation.
  • TABLE 1
    Primarily diluted Final Alcohol Sweetener
    rectified spirit Sugar Allulose volume concentration concentration
    Note 50 vol % — — — — —
    Unit mL g g mL vol % g/L %
    Comparative 160 10 1000  8.0% 1.0%
    Example 1
    Comparative 260 10 1000 13.0% 1.0%
    Example 2
    Comparative 360 10 1000 18.0% 1.0%
    Example 3
    Example 1 160 10 1000  8.0% 1.0%
    Example 2 260 10 1000 13.0% 1.0%
    Example 3 360 10 1000 18.0% 1.0%
  • TABLE 2
    Score of smell or
    Primarily diluted Final Alcohol Sweetener bitterness
    rectified spirit Sugar Allulose volume concentration concentration intensity of
    Note 50 vol % — — — — — alcohol in sensory
    Unit mL g g mL vol % g/L % evaluation
    Sensory 100 1000  5.0% 0.0% 1
    comparison
    A1
    Sensory 120 1000  6.0% 0.0% 3
    comparison
    A2
    Sensory 140 1000  7.0% 0.0% 5
    comparison
    A3
    Sensory 160 1000  8.0% 0.0% 7
    comparison
    A4
    Sensory 180 1000  9.0% 0.0% 9
    comparison
    A5
    Sensory 200 1000 10.0% 0.0% 1
    comparison
    B1
    Sensory 220 1000 11.0% 0.0% 3
    Comparison
    B2
    Sensory 240 1000 12.0% 0.0% 5
    Comparison
    B3
    Sensory 260 1000 13.0% 0.0% 7
    comparison
    B4
    Sensory 280 1000 14.0% 0.0% 9
    comparison
    B5
    Sensory 300 1000 15.0% 0.0% 1
    comparison
    C1
    Sensory 320 1000 16.0% 0.0% 3
    comparison
    C2
    Sensory 340 1000 17.0% 0.0% 5
    comparison
    C3
    Sensory 360 1000 18.0% 0.0% 7
    comparison
    C4
    Sensory 380 1000 19.0% 0.0% 9
    comparison
    C5
  • (2) Sensory Evaluation
  • Sensory evaluation was carried out by 15 trained panelists. Here, the panelists were provided with the standard products as Sensory Comparison Examples (Table 2) and trained to be fully aware of an unpleasant bitterness and smell inherent to alcohol and compare intensity of the bitterness and smell to match a randomly offered diluted soju sample to the alcohol concentration thereof.
  • Specifically, sensory evaluation was carried out through the following procedure. First, the panelists were placed in separate rooms, respectively, and then provided with Sensory Comparison Examples to be aware of the bitterness and smell inherent to alcohol, once more, prior to being provided with the samples of Examples and Comparative Examples. Thereafter, the samples of Example 1 and Comparative Example 1, each labelled with a three-digit random number, were provided in a transparent cup one by one in a double-blind fashion. During evaluation, each of the panelists was instructed to hold the sample in his/her mouth to taste the sample before spitting the sample out, to be prevented from becoming intoxicated due to excessive intake. In addition, after evaluation of each sample, the panelists were allowed to rinse their mouths with cooked rice and water to prepare for evaluation of the next sample. Each of the trained panelists evaluated intensity of the smell and bitterness of alcohol of each of the samples of Examples and Comparative Examples on a 9-point scale (as shown in Table 2) with reference to Sensory Comparison Examples.
  • For evaluation of Example 1 and Comparative Example 1, evaluation of Example 2 and Comparative Example 2, and evaluation of Example 3 and Comparative Example 3, Sensory Comparison Examples A1 to A5, Sensory Comparison Examples B1 to B5, and Sensory Comparison Examples C1 to C5 were provided, respectively. In addition, after each evaluation, the panelists were allowed to rest for at least 12 to 24 hours to avoid confusion due to excessive intake.
  • For the samples of Example 1 and Comparative Example 1, the samples of Example 2 and Comparative Example 2, and the samples of Example 3 and Comparative Example 3, scores of intensity of the smell or bitterness of alcohol in sensory evaluation (hereinafter, “sensory scores”) were converted into alcohol concentration according to Equation 1, Equation 2, and Equation 3, respectively, thereby calculating perceived alcohol concentrations, which in turn were compared with the actual alcohol concentrations to confirm reduction effects in smell and bitterness of alcohol.

  • Perceived alcohol concentration (%)=(0.005×sensory score+0.045)×100   <Equation 1>

  • Perceived alcohol concentration (%)=(0.005×sensory score+0.095)×100   <Equation 2>

  • Perceived alcohol concentration (%)=(0.005×sensory score+0.145)×100   <Equation 3>
  • Statistical analysis was performed using SAS 9.1 program (SAS Inc., Cary, N.C., USA), and sensory evaluation results were analyzed by t-test method. All analysis results were approved at a significance level of p<0.05.
  • As a result, it was confirmed that the samples of Examples had a perceived alcohol concentration (i.e., intensity of smell and bitterness of alcohol) that is about 0.6% to 0.8% lower than the actual alcohol concentration. In addition, it could be seen that the samples of Examples 1 to 3 containing allulose exhibited a statistically significant decrease (p<0.05) in smell and bitterness of alcohol, as compared with those of Comparative Examples 1 to 3 containing the same amount of saccharides.
  • TABLE 3
    Comparative Comparative Comparative
    Sample Example 1 Example 1 Example 2 Example 2 Example 3 Example 3
    Actual alcohol 8.0% 13.0% 18.0%
    concentration
    Perceived alcohol 7.8% 7.4% 13.1% 12.2% 17.9% 17.3%
    concentration
    (bitterness)
    p-value 0.013 0.000 0.001
  • TABLE 4
    Comparative Comparative Comparative
    Sample Example 1 Example 1 Example 2 Example 2 Example 3 Example 3
    Actual alcohol 8.0% 13.0% 18.0%
    concentration
    Perceived alcohol 7.9% 7.5% 13.1% 12.3% 18.0% 17.4%
    concentration
    (smell)
    p-value 0.021 0.000 0.001
    • EXPERIMENTAL EXAMPLE 2 Preparation and Quality Analysis of Diluted Soju Having Different Saccharide Concentrations
  • In order to confirm saccharide concentration-dependent reduction effects in smell and bitterness of alcohol, changes in quality of diluted soju with varying amount of added allulose were checked for a given alcohol concentration. Here, the alcohol concentration was set to 18%, which is an alcohol concentration of the most typical diluted soju in Korea.
  • (1) Preparation of Diluted Soju Having Different Saccharide Concentrations
  • Samples of Comparative Examples 4 to 8, samples of Examples 4 to 8, and Sensory Comparison Examples D1 to D5 were prepared in the same manner as in Experimental Example 1 except that amounts of saccharides were changed as listed in Tables 5 and 6.
  • TABLE 5
    Primarily diluted Final Alcohol Sweetener
    rectified spirit Sugar Allulose volume concentration concentration
    Note 50 vol % — — — — —
    Unit mL g g mL vol % g/L %
    Comparative 350 1 1000 18.0% 0.10%
    Example 4
    Comparative 350 3 1000 18.0% 0.30%
    Example 5
    Comparative 350 10 1000 18.0% 1.00%
    Example 6
    Comparative 350 30 1000 18.0% 3.00%
    Example 7
    Comparative 350 100 1000 18.0% 10.00% 
    Example 8
    Example 4 350 1 1000 18.0% 0.10%
    Example 5 350 3 1000 18.0% 0.30%
    Example 6 350 10 1000 18.0% 1.00%
    Example 7 350 30 1000 18.0% 3.00%
    Example 8 350 100 1000 18.0% 10.00% 
  • TABLE 6
    Score of smell or
    Primarily diluted Final Alcohol Sweetener bitterness
    rectified spirit Sugar Allulose volume concentration concentration intensity of
    Note 50 vol % — — — — — alcohol in sensory
    Unit mL g g mL vol % g/L % evaluation
    Sensory 300 1000 15.0% 0.0% 1
    comparison
    D1
    Sensory 320 1000 16.0% 0.0% 3
    comparison
    D2
    Sensory 340 1000 17.0% 0.0% 5
    comparison
    D3
    Sensory 360 1000 18.0% 0.0% 7
    comparison
    D4
    Sensory 380 1000 19.0% 0.0% 9
    comparison
    D5
  • (2) Sensory Evaluation
  • Sensory evaluation of Examples 4 to 8 and Comparative Examples 4 to 8 was performed in the same manner as in Experimental Example 1. Sensory scores were converted into alcohol concentration according to Equation 3, thereby calculating perceived alcohol concentrations, which, in turn, were compared with the actual alcohol concentrations, respectively, to confirm reduction effects in smell and bitterness of alcohol. Statistical analysis was performed using SAS 9.1 program (SAS Inc., Cary, N.C., USA), and sensory evaluation results were analyzed by t-test method.
  • As a result, it was confirmed that the samples of Examples 4 to 7 having an allulose content of 0.1% to 3.0% exhibited a statistically significant decrease in alcohol bitterness, as compared with those of Comparative Examples containing the same amount of sugar, and the samples of Examples 5 to 7 having an allulose content of 0.3% to 3.0% exhibited a statistically significant decrease in alcohol smell, as compared with those of Comparative Examples (Table 7, FIGS. 1 and 2).
  • In addition, it was confirmed entirely that bitterness of alcohol decreased due to increase in sweetness with increasing concentration of sugar or allulose. However, considering that the sweetness intensity of allulose is about 70% that of sugar, it can be seen that reduction effects in smell and bitterness of alcohol is not due to simply sweetness enhancement by addition of saccharides but due to efficacy of allulose.
  • TABLE 7
    Comp. Comp. Comp. Comp. Comp.
    Sample Example 4 Example 4 Example 5 Example 5 Example 6 Example 6 Example 7 Example 7 Example 8 Example 8
    Actual 18.0% 18.0% 18.0% 18.0% 18.0%
    alcohol
    concentration
    Saccharide  0.1%  0.3%  1.0%  3.0% 10.0%
    concentration
    Perceived 18.1% 17.8% 18.0% 17.7% 17.8% 17.3% 17.5% 16.9% 16.6% 16.3%
    alcohol
    concentration
    (bitterness)
    p-value 0.093 0.011 0.001 0.000 0.064
    Perceived 18.0% 17.8% 17.9% 17.6% 17.6% 17.1% 17.4% 16.8% 16.7% 16.4%
    alcohol
    concentration
    (smell)
    p-value 0.029 0.008 0.000 0.000 0.077

Claims (10)

What is claimed is:
1. An agent for improving an alcohol flavor of an alcoholic beverage, comprising allulose-containing saccharides and ethanol.
2. The agent according to claim 1, wherein the ethanol is present in an amount of 6 vol % to 70 vol % based on the total volume of the alcoholic beverage.
3. The agent according to claim 1, wherein the alcoholic beverage is a distillated alcoholic beverage.
4. The agent according to claim 1, wherein the alcohol flavor is a bitterness or smell of alcohol.
5. The agent according to claim 1, wherein improving the alcohol flavor comprises reducing or masking the alcohol flavor.
6. The agent according to claim 1, wherein the allulose is present in an amount of 50 parts by weight to 100 parts by weight relative to 100 parts by weight of the saccharides in terms of dried solid content.
7. The agent according to claim 1, wherein the allulose-containing saccharides is present in an amount of 0.1 g to 10 g per 1 L of the alcoholic beverage.
8. The agent according to claim 1, wherein the allulose is present in an amount of 5 g to 600 g per 1 L of the ethanol.
9. A method of improving an alcohol flavor of an alcoholic beverage, comprising:
adding allulose to the alcoholic beverage; or
adding allulose to a beverage, followed by adding ethanol to the beverage.
10. Use of allulose-containing saccharides for improving an alcohol flavor of an alcoholic beverage.
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