US20220009957A1

US20220009957A1 - Methods for Preparing Bile Acids

Info

Publication number: US20220009957A1
Application number: US17/381,865
Authority: US
Inventors: Dejun Liu; Yingzhe Qian; JunHo Lee; Yoonseok Song
Original assignee: Medy Tox Inc
Current assignee: Wuxi Apptec Hong Kong Ltd; Medy Tox Inc
Priority date: 2017-08-03
Filing date: 2021-07-21
Publication date: 2022-01-13
Also published as: EP3645548B1; KR20220035196A; JP2020528920A; JP6937893B2; CN111328332A; KR102388809B1; CA3070853C; BR112020002300A2; CA3070853A1; US11161872B2; US20200369712A1; EP3645548C0; WO2019024920A1; EP3645548A4; CN111328332B; EP3645548A1; KR20200026273A

Abstract

The present disclosure provides methods of preparing cholic acid, deoxycholic acid, or chenodeoxycholic acid, an ester thereof, or a pharmaceutically or cosmetically acceptable salt thereof, and novel and useful synthetic intermediates, for example, as described for methods 1, 1A, 1B, 2, 3, 3A, and 4. The method can start with a plant derived steroid as a starting material, such as dehydroepiandrosterone (DHEA) or Acetyl-dehydroepiandrosterone (Ac-DHEA). Also provided are pharmaceutical or cosmetic compositions and uses thereof, which comprise one or more of cholic acid, deoxycholic acid, or chenodeoxycholic acid, an ester thereof, or a pharmaceutically or cosmetically acceptable salt thereof, which is of high purity, for example, free of animal derived impurities.

Description

CROSS-REFERENCE TO RELATED APPLICATION

This application is a continuation of U.S. application Ser. No. 16/636,260, which is a 35 U.S.C. § 371 national phase of PCT/CN2018/098535, having an international filing date of Aug. 3, 2018, which claims priority to PCT/CN2017/095784, filed on Aug. 3, 2017, the contents of which are herein incorporated by reference in their entirety.

BACKGROUND OF THE INVENTION

Field of the Invention

The present disclosure is generally related to the field of steroid synthesis, e.g., methods of preparing a bile acid (e.g., cholic acid, deoxycholic acid, or chenodeoxycholic acid) from a plant derived steroid, and novel synthetic intermediates. The present disclosure is also related to pharmaceutical or cosmetic compositions comprising a bile acid (e.g., cholic acid, deoxycholic acid, or chenodeoxycholic acid) or its ester(s) or salt(s), and pharmaceutical or cosmetic uses thereof.

Background Art

Bile acids such as deoxycholic acid, cholic acid and the like are pharmaceutical and cosmetically useful compounds. See, e.g., Mukhopadhyay, S. and U. Maitra., Current Science 87:1666-1683 (2004) (“Chemistry and Biology of Bile Acids”). Recently, the use of one or more of cholate and chenodeoxycholate has been suggested for non-surgical removal of a localized fat deposit in a subject. See, e.g., WO 2015/198150. Bile acids were typically obtained from bovine and ovine sources. Pathogens such as prions can contaminate such sources. Thus, stringent conditions for mammalian sourced bile acids are required by regulatory authorities. See, e.g., WO 2008/157635 and WO 2011/075701. For example, deoxycholic acid from animals in New Zealand are a source of bile acids for human use under US regulatory regimes, as long as the animals continue to remain isolated and otherwise free of observable pathogens. Such stringent conditions impose a limitation on the amount of suitable mammalian sourced bile acids and do not preclude the possibility that the bile acids will be free of such pathogens.
In light of the stringent conditions for mammalian sourced bile acids noted above, synthetic bile acids such as cholic acid, deoxycholic acid, chenodeoxycholic acid, suitable for use as medicaments or cosmetics in humans are in need. These synthetic bile acids would be synthesized starting from non-animal sources, such as from plant sources or synthetic starting materials. Therefore, the synthetic bile acids can be advantageous at least in that they are known from the outset to be free from animal derived impurities, such as pathogens that are capable of producing a deleterious effect on a human, and other harmful agents such as animal or microbial metabolites, toxins, including bacterial toxins, such as pyrogens.

BRIEF SUMMARY OF THE INVENTION

In various embodiments, the present disclosure is directed to a method of preparing cholic acid, deoxycholic acid, or chenodeoxycholic acid, an ester thereof, or a pharmaceutically or cosmetically acceptable salt thereof. In some embodiments, the method can comprise, consisting essentially of, or consisting of any of the embodiments described below for any of methods 1, 1A, 1, 2, 3, 3A, and 4. In some embodiments, the method can also comprise, consisting essentially of, or consisting of any of the embodiments according to any of Schemes 1-8. In some embodiments, the method uses a non-animal derived steroid (e.g., plant derived, or synthetic) as a starting material. In some embodiments, the method starts with a plant derived steroid as a starting material, such as dehydroepiandrosterone (DHEA) or Acetyl-dehydroepiandrosterone (Ac-DHEA). In some embodiments, the method starts with a steroid starting material that is free of any animal derived impurities. In some embodiments, the method produces the cholic acid, deoxycholic acid, or chenodeoxycholic acid, ester thereof, or pharmaceutically or cosmetically acceptable salt thereof that is free of any animal derived impurities. Thus, certain embodiments of the present disclosure are also directed to cholic acid, deoxycholic acid, or chenodeoxycholic acid, an ester thereof, or a pharmaceutically or cosmetically acceptable salt thereof, produced by any of the methods described herein.
In some embodiments, the present disclosure also provides novel synthetic intermediates useful for the preparation of cholic acid, deoxycholic acid, or chenodeoxycholic acid, an ester thereof, or a pharmaceutically or cosmetically acceptable salt thereof.
In some embodiments, the present disclosure also provides a high purity cholic acid composition. In some embodiments, the high purity cholic acid composition comprises, consists essentially of, or consists of, cholic acid or a salt thereof, and one or more compounds selected from Compounds 32-35, or a salt thereof. In some embodiments, the total amount of Compounds 32-35 and salts thereof, as applicable, is less than 5% (e.g., less than 5%, less than 4%, less than 3%, less than 2%, less than 1%, less than 0.5%, less than 0.2%, less than 0.1%) by weight and/or by HPLC area of the cholic acid and salt thereof. In some embodiments, the amount of each of Compounds 32-35 and its respective salt, as applicable, is less than 5% (e.g., less than 5%, less than 4%, less than 3%, less than 2%, less than 1%, less than 0.5%, less than 0.2%, less than 0.1%) by weight and/or by HPLC area of the cholic acid and salt thereof. In some embodiments, the cholic acid or salt thereof has a purity of at least 95% (e.g., at least 96%, at least 97%, at least 98%, or at least 99%) by weight and/or by HPLC area. In some embodiments, the high purity cholic acid composition is substantially free or free from an animal derived impurity.
In some embodiments, the present disclosure also provides a pharmaceutical or cosmetic composition comprising one or more of high purity cholic acid, deoxycholic acid, or chenodeoxycholic acid, an ester thereof, or a pharmaceutically or cosmetically acceptable salt thereof. In some embodiments, the pharmaceutical or cosmetic composition comprises an effective amount of high purity (e.g., a purity of at least 98%, at least 99%, at least 99.5%, or at least 99.9% by weight) cholic acid or a pharmaceutically or cosmetically acceptable salt thereof, or a high purity cholic acid composition described herein, and a pharmaceutically or cosmetically acceptable excipient. In some embodiments, the pharmaceutical or cosmetic composition further comprises an effective amount of high purity (e.g., a purity of at least 98%, at least 99%, at least 99.5%, or at least 99.9% by weight) deoxycholic acid or chenodeoxycholic acid, or a pharmaceutically or cosmetically acceptable salt thereof. In any of the embodiments described herein, the high purity cholic acid, deoxycholic acid or chenodeoxycholic acid, or an ester or a pharmaceutically or cosmetically acceptable salt thereof can also be characterized by being free of any animal derived impurities.
Some embodiments of the present disclosure are directed to a method of administering the pharmaceutical or cosmetic composition described herein. In some embodiments, the method is for non-surgical removal of a localized fat deposit in a subject. In some embodiments, the method comprises administering to the subject a pharmaceutical or cosmetic composition comprising a fat-lysing effective amount of high purity (e.g., a purity of at least 98%, at least 99%, at least 99.5%, or at least 99.9% by weight) cholic acid, chenodeoxycholic acid, a pharmaceutically or cosmetically acceptable salt thereof, or a combination thereof, and a pharmaceutically or cosmetically acceptable excipient.
Additional embodiments and advantages of the disclosure will be set forth, in part, in the description that follows, and will flow from the description, or can be learned by practice of the disclosure. The embodiments and advantages of the disclosure will be realized and attained by means of the elements and combinations particularly pointed out in the appended claims.
It is to be understood that both the foregoing summary and the following detailed description are exemplary and explanatory only, and are not restrictive of the invention as claimed.

DESCRIPTION OF THE DRAWINGS/FIGURES

FIG. 1 shows a drawing of X-ray structure for Compound 35.

FIG. 2 presents one exemplary HPLC trace showing an impurity profile of cholic acid synthesized by the methods herein (e.g., as shown in Examples 1 and 2). In the HPLC trace, Compound 32 is designated as impurity 1, which has a retention time of 5.02 minutes, Compound 33 is designated as impurity 2, which has a retention time of 9.17 minutes, Compound 34 is designated as impurity 3, which has a retention time of 12.10 minutes, and Compound 35 is designated as impurity 4, which has a retention time of 15.73 minutes.

DETAILED DESCRIPTION OF THE INVENTION

Definitions

It is meant to be understood that proper valences are maintained for all moieties and combinations thereof.
It is also meant to be understood that a specific embodiment of a variable moiety herein may be the same or different as another specific embodiment having the same identifier. For example, R¹in various formulae is independently selected and can be the same or different. Other variables such as R³⁰in various formulae are also independently selected and can be the same or different.
As used herein, the term “animal derived impurities” refers to impurities having an “animal origin,” which refers to originating from a kingdom (Animalia) of living things including multi-celled organisms and single celled organisms. In some embodiments, the term “animal derived impurities” can be pathogens, animal or microbial metabolites, and toxins, including bacterial toxins, such as pyrogens.
As used herein, the term “cholic acid (CA)” refers to the compound 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid, which has the following structure:
As used herein, a “steroid starting material” refers to a compound having a steroid ring skeleton as follows:
Throughout the present disclosure, the atom and ring numbering in steroid skeletons of the compounds herein follows the convention below:
As used herein, the term “chenodeoxycholic acid (CDCA)” refers to the compound 3α,7α-dihydroxy-5β-cholan-24-oic acid, which has the following structure:
As used herein, the term “deoxycholic acid (DCA)” refers to the compound 3α,12α-dihydroxy-5β-cholan-24-oic acid, which has the following structure:
As used herein, the term “pharmaceutically acceptable salt” refers to a salt which is, suitable for pharmaceutical use and is commensurate with a reasonable benefit/risk ratio. Pharmaceutically acceptable salts are known in the art. For example, the bile acids (e.g., CA, DCA, or CDCA) described herein can be in the form of a sodium, potassium, lithium, calcium, magnesium, ammonium, or tetraalkylammonium salt. Suitable salts include those described in P. Heinrich Stahl, Camille G. Wermuth (Eds.), Handbook of Pharmaceutical Salts Properties, Selection, and Use; 2002. These pharmaceutically acceptable salts can be prepared by reacting the bile acids (e.g., CA, DCA, or CDCA) with a suitable base. For illustrative purposes, examples of such bases include sodium hydroxide, potassium hydroxide, or lithium hydroxide. Alternatively, the salts can be prepared by hydrolysis of esters of the bile acids (e.g., CA, DCA, or CDCA) with a base and omitting an acidic workup that would lead to the acid form of the bile acids.
As used herein, the term “cosmetically acceptable salt” refers to a salt which is suitable for cosmetic use and is commensurate with a reasonable benefit/risk ratio. Cosmetically acceptable salts are known in the art and include, for example, base addition salts with a cation selected from the group consisting of sodium (Na⁺), potassium (K⁺), lithium (Li⁺), magnesium (Mg²⁺), calcium (Ca²⁺), barium (Ba²⁺), strontium (Sr²⁺), and ammonium (NH₄ ⁺).
The term “subject” as used herein refers to any animal, or cells thereof whether in vitro or in vivo, amenable to the methods described herein. In preferred embodiments, the subject is a human.
As used herein, the term “alkyl” refers to a radical of a straight-chain or branched saturated hydrocarbon group having from 1 to 10 carbon atoms (“C_1-10alkyl”). In some embodiments, an alkyl group has 1 to 9 carbon atoms (“C_1-9alkyl”). In some embodiments, an alkyl group has 1 to 8 carbon atoms (“C_1-8alkyl”). In some embodiments, an alkyl group has 1 to 7 carbon atoms (“C_1-7alkyl”). In some embodiments, an alkyl group has 1 to 6 carbon atoms (“C_1-6alkyl”). In some embodiments, an alkyl group has 1 to 5 carbon atoms (“C_1-5alkyl”). In some embodiments, an alkyl group has 1 to 4 carbon atoms (“C_1-4alkyl”). In some embodiments, an alkyl group has 1 to 3 carbon atoms (“C_1-3alkyl”). In some embodiments, an alkyl group has 1 to 2 carbon atoms (“C_1-2alkyl”). In some embodiments, an alkyl group has 1 carbon atom (“C₁alkyl”). In some embodiments, an alkyl group has 2 to 6 carbon atoms (“C_2-6alkyl”). Examples of C_1-6alkyl groups include methyl (C₁), ethyl (C₂), propyl (C₃) (e.g., n-propyl, isopropyl), butyl (C₄) (e.g., n-butyl, tert-butyl, sec-butyl, iso-butyl), pentyl (C₅) (e.g., n-pentyl, 3-pentanyl, amyl, neopentyl, 3-methyl-2-butanyl, tertiary amyl), and hexyl (C₆) (e.g., n-hexyl). Additional examples of alkyl groups include n-heptyl (C₇), n-octyl (C₈), and the like.
As used herein, the term “optionally substituted alkyl” refers to an alkyl group that is unsubstituted or substituted (a “substituted alkyl”) with one or more substituents (e.g., as described herein, e.g., halogen, such as F).
As used herein, the term “hydroxyl protecting agent” refers to a reagent which under suitable reaction conditions reacts with a hydroxyl group to form a hydroxyl protecting group.
As used herein, the term “hydroxyl protecting group” refers to any group which forms a derivative of the hydroxyl group that is stable to the projected reaction(s), wherein said hydroxyl protecting group subsequently optionally can be selectively removed. The hydroxyl derivative can be obtained by selective reaction of a hydroxyl protecting agent with a hydroxyl group. A person of ordinary skill in the art can select proper hydroxyl protecting group(s) based on the nature of the protecting group(s) and the projected reaction(s). For example, methods for introducing and removing various hydroxyl protecting groups can be found in “Protective Groups in Organic Synthesis”, 4^thed. P. G. M. Wuts; T. W. Greene, John Wiley, 2007, and in P. J. Kocienski, “Protecting Groups”, 3^rded. G. Thieme, 2003, the contents of which are hereby incorporated by reference in their entirety.
Non-limiting examples of hydroxyl protecting groups suitable for the present disclosure include those forming an ether of the hydroxyl group, such as methyl ether, allyl ether, prenyl ether, p-methoxybenzyl ether, triphenylmethyl ether, 2-trimethylsilylethyl ether, tert-butyl ether, cinnamyl ether, propargyl ether, p-methoxyphenyl ether, benzyl ether, 3,4-dimethoxybenzyl ether, 2,6-dimethoxybenzyl ether, o-nitrobenzyl ether, p-nitrobenzyl ether, 4-(trimethylsilylmethyl)-benzyl ether, 2-naphthylmethyl ether, diphenylmethyl ether, (4-methoxyphenyl)-phenylmethyl ether, (4-phenyl-phenyl)-phenylmethyl ether, p,p′-dinitrobenzhydryl ether, 5-dibenzosuberyl ether, tris(4-tert-butylphenyl)methyl ether, (α-naphthyl)-diphenylmethyl ether, p-methoxyphenyldiphenylmethyl ether, di(p-methoxyphenyl)phenylmethyl ether, tri(p-methoxyphenyl)methyl ether and 9-(9-phenyl)xanthenyl ether.
Non-limiting examples of hydroxyl protecting groups suitable for the present disclosure also include those forming an alkoxyalkylether (e.g., acetals and ketals) of the hydroxyl group, such as 1-ethoxyethyl ether, 1-methyl-1-methoxyethyl ether, [(3,4-dimethoxybenzyl)oxy]methyl ether, guaiacolmethyl ether, 2-methoxyethoxymethyl ether, 2-(trimethylsilyl)ethoxymethyl ether, tetrahydropyranyl ether, tetrahydrofuranyl ether, methoxymethyl ether benzyloxymethyl ether, p-methoxybenzyloxymethyl ether, p-nitrobenzyloxymethyl ether, o-nitrobenzyloxymethyl ether, (4-methoxyphenoxy)methyl ether, tert-butoxymethyl ether, 4-pentenyloxymethyl ether, siloxymethyl ether, 1-methoxycyclohexyl ether, 4-methoxytetrahydropyranyl ether, 1-[(2-chloro-4-methyl)phenyl]-4-methoxypiperidin-4-yl ether, 1-(2-fluorophenyl)-4-methoxypiperidin-4-yl ether, 1-(4-chlorophenyl)-4-methoxypiperidin-4-yl ether or 1-methyl-1-benzyloxyethyl ether.
Non-limiting examples of hydroxyl protecting groups suitable for the present disclosure also include those forming a thioacetal or thioketal of the hydroxyl group, such as tetrahydrothiopyranyl ether, 4-methoxytetrahydrothiopyranyl ether, tetrahydrothiofuranyl ether or 1,3-benzodithiolan-2-yl ether.
Non-limiting examples of hydroxyl protecting groups suitable for the present disclosure also include those forming a silyl ether of the hydroxyl group, such as trimethylsilyl ether, triethylsilyl ether, triisopropylsilyl ether, tert-butyldimethylsilyl ether, dimethylisopropylsilyl ether, diethylisopropylsilyl ether, diphenylmethylsilyl ether, triphenylsilyl ether, dimethylthexylsilyl ether, 2-norbornyldimethylsilyl ether, tert-butyldiphenylsilyl ether, (2-hydroxystyryl)dimethylsilyl ether, (2-hydroxystyryl)diisopropylsilyl ether, tert-butylmethoxyphenylsilyl ether or tert-butoxydiphenylsilyl ether.
Non-limiting examples of hydroxyl protecting groups suitable for the present disclosure also include those forming an ester of the hydroxyl group, such as acetate ester, chloroacetate ester, trifluoroacetate ester, phenoxyacetate ester, formate ester, benzoylformate ester, dichloroacetate ester, trichloroacetate ester, methoxyacetate ester, p-chlorophenoxyacetate ester, phenylacetate ester, 3-phenylpropionate ester, 4-pentenoate ester, 4-oxopentanoate ester, pivaloate ester, crotonate ester, 4-methoxycrotonate ester, angelate ester, benzoate ester or p-phenylbenzoate ester.
Non-limiting examples of hydroxyl protecting groups suitable for the present disclosure also include those forming a carbonate of the hydroxyl group, such as methoxymethyl carbonate, 9-fluorenylmethyl carbonate, methyl carbonate, ethyl carbonate, 2,2,2-trichloroethyl carbonate, 2-(trimethylsilyl)ethyl carbonate, vinyl carbonate, allyl carbonate or p-nitrophenyl carbonate.
Non-limiting examples of hydroxyl protecting groups suitable for the present disclosure also include those forming a sulfinate of the hydroxyl group, such as 2,4-dinitrophenylsulfenate.
As used herein, the term “olefin forming reagent” refers to a regent that can react with a ketone or aldehyde to form an olefin bond under suitable conditions. In some embodiments, the olefin forming reagent can be a Wittig reagent. In some embodiments, the olefin forming reagent can be a phosphorous ylide, or a precursor thereof, e.g., a phosphonium salt that can form the phosphorus ylide under certain conditions (e.g., using a base). In some embodiments, the olefin forming reagent comprises an organometallic reagent (e.g., an organozinc reagent, such as a Reformatsky reagent), wherein the ketone or aldehyde can be converted into an olefin by first forming an alcoholic intermediate with the organometallic reagent, which is followed by dehydration.
As used herein, the term “ylide” refers to a neutral dipolar molecule containing a formally negatively charged atom (usually a carbanion) directly attached to a heteroatom with a formal positive charge (usually nitrogen, phosphorus or sulfur). Thus, when the heteroatom is phosphine, the ylide is a “phosphorous ylide.” For example, methylenetriphenylphosphorane (Ph₃P═CH₂) is a phosphorous ylide.
As used herein, the term “Lewis acid” refers to an electron pair acceptor. Lewis acids include boron reagent such as BF₃(e.g., BF₃Et₂O) and organometallic reagents such as alkyl aluminum halides (e.g. Et₂AlCl and MeAlCl₂).
Synthesis of Cholic Acid, Deoxycholic Acid, and Chenodeoxycholic Acid
Cholic acid includes three hydroxyl groups at positions 3, 7, and 12 of the steroid skeleton. Methods for synthesizing cholic acid can start from a 3,7,12-trioxygenated, a 3,7-deoxygenated or 3,12-deoxygenated, or a 3-oxygenated steroid as a starting material. In some embodiments, cholic acid can be synthesized from a 3,12-deoxygenated steroid starting material. In some embodiments, cholic acid can be synthesized from a 3,7,12-trioxygenated steroid starting material. In some embodiments, cholic acid can be synthesized from a 3,7-deoxygenated steroid starting material. In any of the embodiments described herein, cholic acid can be synthesized from DHEA or Ac-DHEA, which contains a 3-hydroxy or 3-OAc group. Further, methods for preparing cholic acid from DHEA or Ac-DHEA can involve, for example, two broad strategies: (1) introducing the 12-hydroxy group or protected version thereof, and followed by introduction of the 7-hydroxy group or protected version thereof, or (2) introducing the 7-hydroxy group or protected version thereof, and followed by introduction of the 12-hydroxy group or protected version thereof.
Deoxycholic acid has two hydroxyl groups at the 3 and 12 positions and can be synthesized from a 3,12-deoxygenated, or a 3-oxygenated steroid as a starting material. In some embodiments, strategy (1) for cholic acid synthesis from DHEA or Ac-DHEA can also be adapted for the synthesis of deoxycholic acid.
Chenodeoxycholic acid, on the other hand, has two hydroxyl groups at the 3 and 7 positions and can be synthesized from a 3,7-deoxygenated, or a 3-oxygenated steroid as a starting material. Thus, strategy (2) for cholic acid synthesis from DHEA or Ac-DHEA can be adapted for the synthesis of chenodeoxycholic acid.
In some embodiments, the present disclosure also provides cholic acid, deoxycholic acid, or chenodeoxycholic acid, an ester thereof, or a pharmaceutically or cosmetically acceptable salt thereof, produced by any of the methods described herein.
Method 1: Synthesis of Cholic Acid from 3,12-deoxygenated Steroids
Some embodiments of the present disclosure are directed to a method of preparing cholic acid, an ester thereof, or a pharmaceutically or cosmetically acceptable salt thereof from a 3,12-deoxygenated steroid starting material. A 3,12-deoxygenated steroid starting material refers to a compound having a steroid skeleton with each of the 3 and 12 positions a hydroxyl group or protected version thereof, or a ketone group or protected ketone. For example, in some embodiments, the 3,12-deoxygenated steroid can be a 3,12-diketone compound, a 3,12-dihydroxy compound, or a hydroxyketone compound, having the following ring structure (side chains and other functional groups omitted):
In some embodiments, the 3,12-deoxygenated steroid is a 3,12-diketone compound. For example, the method can comprise a reaction sequence shown in Scheme 1:
wherein R¹is a H or an optionally substituted alkyl.
In some embodiments, the method comprises:
a) converting a compound of Formula I-1 into a compound of Formula I-2,

- wherein R¹is H or an optionally substituted alkyl group,
  b) oxidizing the compound of Formula I-2 to form a compound of Formula I-3,

c) reacting the compound of Formula I-3 with an epoxide forming reagent to form an epoxide of Formula I-4
d) reducing the epoxide of Formula I-4 under hydrogenation condition to form a diketone of Formula I-5
e) contacting the diketone of Formula I-5 with a ketone reducing agent to form a compound of Formula I-6
and
f) optionally, when R¹is not H, hydrolyzing the compound of Formula I-6 to provide cholic acid.
In some embodiments, R¹is hydrogen. However, for ease of purification, in preferred embodiments, R¹is a C_1-6alkyl (e.g., methyl or ethyl). In some embodiments, R¹can also be an optionally substituted alkyl (e.g., an optionally substituted C_1-6alkyl). For example, R¹can be an alkyl optionally substituted by 1-3 (e.g., 1, 2, or 3) substituents, wherein the 1-3 substituents are independently a C_1-6alkyl, C_2-6alkenyl, C_2-6alkynyl, C_3-6carbocyclyl, 3-6 membered heterocyclyl, 5 or 6 membered heteroaryl, phenyl, a substituted phenyl (e.g., para-methoxyphenyl), hydroxyl, C_1-6alkoxy (e.g., a methoxy, ethoxy, etc.), halogen (e.g., F or Cl), or amino optionally substituted by one or two groups independently selected from C_1-6alkyl. In preferred embodiments, R¹is a C_1-6alkyl (e.g., methyl or ethyl). The R¹group in Formula I-1 to I-6 can be the same or different. In some embodiments, R¹group in Formula I-1 to I-6 can be the same, for example, as a C_1-6alkyl (e.g., methyl or ethyl).
Various conditions for effecting the transformations according to Scheme 1 or a), b), c), d), e), and f) described above can be used, which are exemplified in embodiments below and in the Examples section. Other useful conditions and details include those known in the art for analogous transformations.
Certain embodiments of the present disclosure are directed to a method of converting the compound of Formula I-1 into the compound of Formula I-2. Compounds of Formula I-1 useful for this conversion can have a 5-alpha hydrogen or a 5-beta hydrogen. In some embodiments, a substantially pure (e.g., at least 80%, at least 85%, at least 90%, at least 95%, or at least 98% by weight) diastereomer of Formula I-1 can be used, with a 5-alpha hydrogen or a 5-beta hydrogen. In some embodiments, a mixture of diastereomers of Formula I-1 can be used, for example, with a ratio of 5-alpha hydrogen compound to 5-beta hydrogen compound ranging from 50:1 to 1:50. In some embodiments, the converting comprises contacting the compound of Formula I-1 with a halogenating agent to form a halogenated intermediate, and subsequently treating the halogenated intermediate under elimination condition to form the compound of Formula I-2. Halogenating agents suitable for use include those known in the art for introducing an alpha-halogen to a ketone. In some embodiments, the halogenating agent is bromine. In some embodiments, the halogenating agent is N-bromosuccinimide (NBS). The halogenated intermediate formed can have either an alpha or beta configuration:
The 5-position stereochemistry of the halogenated intermediate depends on the corresponding stereochemistry of the starting material, Formula I-1, and can have either an alpha or beta hydrogen. In some embodiments, X is bromine and R¹is an optionally substituted alkyl, e.g., a C_1-6alkyl (e.g., methyl or ethyl). The halogenated intermediate (e.g., brominated intermediate) can then be treated under elimination condition to form the compound of Formula I-2, an alpha-beta-unsaturated ketone. In some embodiments, the halogenated intermediate is purified prior to the treating under elimination condition. However, preferably, the halogenated intermediate is not purified prior to the treating under elimination condition. Suitable elimination conditions include those known in the art for the elimination of HX (X is halogen, e.g., Br) of an alpha-halogenated ketone. For example, in some embodiments, the elimination condition comprises heating the halogenated intermediate, e.g., to a temperature ranging from 120° C. to 180° C. (e.g., 120° C., 130° C., 140° C., 150° C., 160° C., 170° C., 180° C., or any ranges between the recited values), in the presence of a base (e.g., Li₂CO₃) and an agent that facilitates the elimination (e.g., LiBr) in a suitable solvent (e.g., DMF, DMSO). Other methods known in the art for converting a ketone into an alpha-beta-unsaturated ketone can also be used to effect the conversion of the compound of Formula I-1 into the compound of Formula I-2.
Certain embodiments of the present disclosure are directed to a method of converting the compound of Formula I-2 into the compound of Formula I-3, for example, using an oxidizing agent. In some embodiments, the oxidizing agent is a benzoquinone based oxidizing agent such as chloranil (tetrachloro-1,4-benzoquinone) or 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ). In some embodiments, the method comprises contacting the compound of Formula I-2 with chloranil or DDQ to form the compound of Formula I-3.
Certain embodiments of the present disclosure are directed to a method of converting the compound of Formula I-3 into the epoxide of Formula I-4. The compound of Formula I-3 includes olefin double bonds at the 6,7 positions and at the 4,5 positions, which can be converted into the epoxide of Formula I-4 by using an epoxide forming reagent. In some embodiments, the epoxide forming reagent is a peroxycarboxylic acid. In some embodiments, the epoxide forming reagent is meta-chloroperoxybenzoic acid. Other suitable epoxide forming reagents include those known in the art that can selectively produce an epoxide at the 6,7 positions with the olefin at the 4,5 positions intact.
Certain embodiments of the present disclosure are directed to a method of converting the epoxide of Formula I-4 into the diketone of Formula I-5. Various methods for reducing the epoxide can be used. Preferably, the method comprises hydrogenating the epoxide of Formula I-4. In some embodiments, the hydrogenation is catalyzed by a heterogeneous catalyst, for example, a heterogeneous metal catalyst comprising Pd, Ni, Pt, PtO₂, Rh, or Ru. In some embodiments, the heterogeneous metal catalyst is Pd/C. The hydrogenation can be conducted at various H₂pressures. In some embodiments, the hydrogenation is conducted at a hydrogen pressure of at or near 1 atm. In some embodiments, the hydrogenation is conducted at a hydrogen pressure of greater than 1 atm (e.g., about 2 atms, about 3 atms, about 5 atms, about 10 atms, about 15 atms, or any ranges between the recited values). In some embodiments, the hydrogenation is conducted at a hydrogen pressure of about 15 psi to about 100 psi (e.g., about 50 psi).
Certain embodiments of the present disclosure are directed to a method of converting the diketone of Formula I-5 into the compound of Formula I-6, for example, using a ketone reducing agent. In some embodiments, the ketone reducing agent is a borohydride (such as NaBH₄, NaCNBH₃, etc.) or a trialkylaluminum hydride (e.g., tri-isopropoxyaluminum hydride, tri-tert-butoxyaluminum hydride). In preferred embodiments, the ketone reducing agent is lithium tri-tert-butoxyaluminum hydride (LiAlH(O-tBu)₃).
When R¹is not hydrogen, for example, when R¹is a C_1-6alkyl, then to synthesize cholic acid, the method further comprises hydrolyzing the compound of Formula I-6. Thus, in some embodiments, when R¹is not hydrogen, the method comprises hydrolyzing the compound of Formula I-6. Suitable hydrolyzing conditions include those known in the art. For example, the hydrolysis can be effected by reacting the compound of Formula I-6 under acid-mediated (e.g., BF₃mediated), base-mediated (e.g., using an alkali metal hydroxide (e.g., NaOH or LiOH)), or nucleophile-mediated (e.g., I⁻ mediated) hydrolysis conditions. In preferred embodiments, the hydrolysis comprises contacting the compound of Formula I-6 with an alkali metal hydroxide (e.g., NaOH or LiOH).
In some embodiments, an ester of cholic acid, e.g., the compound of Formula I-6 wherein R¹is an optionally substituted alkyl, is the desired product. For example, in some embodiments, the method is directed to the synthesis of a pharmaceutically or cosmetically acceptable ester of cholic acid. In such embodiments, the preferred reaction conditions for sequences a)-e) are those that no or minimal hydrolysis of the ester (i.e., —CO₂R¹) occurs under the respective reaction conditions. However, if desired, the ester of cholic acid can also be prepared by esterification of cholic acid with a desired alcohol.
In some embodiments, a salt of cholic acid is the desired product. For example, in some embodiments, the method is directed to the synthesis of a pharmaceutically or cosmetically acceptable salt of cholic acid. Thus, in some embodiments, the method comprises forming a pharmaceutically or cosmetically acceptable salt of cholic acid, for example, by reacting cholic acid with a suitable base, e.g., metal or ammonium hydroxide (e.g., sodium, potassium, lithium, calcium, magnesium, ammonium, or tetraalkylammonium hydroxide).
The compound of Formula I-1 used in the methods described hereinabove can be prepared via different methods. In any of the embodiments described herein, the compound of Formula I-1 can be prepared by the methods described herein, e.g., Method 1A below. In any of the embodiments described herein, the compound of Formula I-1 can also be a synthetic material free of any animal derived impurities.
Method 1A: Synthesis of 3,12-deoxygenated Steroid of Formula I-1
Certain embodiments of the present disclosure are directed to the synthesis of the compound of Formula I-1, wherein R¹is H or an optionally substituted alkyl group. Formula I-1 can have a 5-alpha hydrogen or a 5-beta hydrogen. In some embodiments, compounds of Formula I-1 exist as a substantially pure (e.g., at least 80%, at least 85%, at least 90%, at least 95%, or at least 98% by weight) diastereomer, with a 5-alpha hydrogen or a 5-beta hydrogen. In some embodiments, compounds of Formula I-1 exist as a diastereomeric mixture in various ratios, for example, with a ratio of 5-alpha hydrogen compound to 5-beta hydrogen compound ranging from 50:1 to 1:50.
In some embodiments, the compound of Formula I-1 can be synthesized according to Scheme 2, wherein each variables and transformations are as described herein:
Certain embodiments are directed to a method of oxidizing a diol of Formula I-7 to provide the compound of Formula I-1
Formula I-7 can have a 5-alpha hydrogen or a 5-beta hydrogen. In some embodiments, compounds of Formula I-7 exist as a substantially pure (e.g., at least 80%, at least 85%, at least 90%, at least 95%, or at least 98% by weight) diastereomer, with a 5-alpha hydrogen or a 5-beta hydrogen. In some embodiments, compounds of Formula I-7 exist as a diastereomeric mixture in various ratios, for example, with a ratio of 5-alpha hydrogen compound to 5-beta hydrogen compound ranging from 50:1 to 1:50. In some embodiments, the oxidizing comprises a Chromium trioxide or chromate based oxidation or variations thereof. In some embodiments, the oxidizing comprises a Swern oxidation or variations thereof. In some embodiments, the oxidizing comprises a hypervalent iodine mediated oxidation. In some embodiments, the oxidizing comprises contacting the diol of Formula I-7 with a suitable oxidizing agent. In some embodiments, the oxidizing agent is a Chromium based oxidizing agent, for example, Jones reagent (a solution of chromium trioxide in dilute sulfuric acid); PCC (pyridinium chlorochromate), etc. In a preferred embodiment, the oxidizing agent is Jones reagent. In some embodiments, the oxidizing agent is a Swern oxidation reagent. As used herein, the term “Swern oxidation reagent” encompasses all activated forms of DMSO, such as a chlorosulfonium active species, that can oxidize an alcohol into a ketone or aldehyde in the presence of an organic base such as triethyl amine or diisopropylethylamine. Various Swern oxidation reagent systems are known. For example, a typical Swern oxidation can include using a DMSO/oxalyl chloride based system, in which DMSO first reacts with oxalyl chloride to generate a chloro(dimethyl)sulfonium chloride, which then converts an alcohol into an aldehyde or ketone group in the presence of an organic base (e.g., Et₃N). Other exemplary useful oxidizing agents include reagents for Swern oxidation or variations thereof which use cyanuric chloride, trifluoroacetic anhydride, carbodiimides, pyridine-sulfur trioxide complex to active DMSO, or use dimethyl sulfide and N-chlorosuccinimide to generate the chlorosulfonium active species. In some embodiments, the oxidizing agent can be 2-iodoxybenzoic acid (IBX) or Dess-Martin periodinane (1,1,1-Triacetoxy-1,1-dihydro-1,2-benziodoxol-3(1H)-one).
In some embodiments, the diol of Formula I-7 can be synthesized by hydrogenating a compound of Formula I-8 or Formula I-8a, or a geometric isomer thereof,
wherein Pg¹and Pg²are independently a hydroxyl protecting group; and optionally removing the hydroxyl protecting groups. Suitable hydroxyl protecting groups include any of those known in the art, for example, those described herein. In some embodiments, Pg¹and Pg²are the same. In some embodiments, Pg¹and Pg²are different. In preferred embodiments, both Pg¹and Pg²are hydroxyl protecting group that forms an ester with the hydroxyl group, for example, both Pg¹and Pg²are acetyl. In some embodiments, the diol of Formula I-7 is synthesized by hydrogenating the compound of Formula I-8a. In some embodiments, the diol of Formula I-7 is synthesized by hydrogenating the compound of Formula I-8, which is followed by removing the hydroxyl protecting groups. In some embodiments, the diol of Formula I-7 is synthesized by removing the hydroxyl protecting groups of the compound of Formula I-8 to produce the compound of Formula I-8a, which is followed by hydrogenating the compound of Formula I-8a.
As the olefin double bonds in Formula I-8 or Formula I-8a are hydrogenated to form the diol of Formula I-7, the geometric isomers of the compounds of Formula I-8 and Formula I-8a can also be used. In some embodiments, the method comprises hydrogenating a compound of Formula I-8 or Formula I-8a. In some embodiments, the method comprises hydrogenating a geometric isomer of the compound of Formula I-8 or Formula I-8a. In some embodiments, the method comprises hydrogenating a mixture of the compound of Formula I-8 or Formula I-8a, and its geometric isomers.
Various methods for hydrogenating the compound of Formula I-8 or Formula I-8a can be used. Preferably, the method comprises hydrogenating the compound of Formula I-8 or Formula I-8a with H₂in the presence of a heterogeneous catalyst, for example, a heterogeneous metal catalyst comprising Pd, Ni, Pt, PtO₂, Rh, or Ru. In some embodiments, the heterogeneous metal catalyst is Pd/C. The hydrogenation can be conducted at various H₂pressures. In some embodiments, the hydrogenation is conducted at a hydrogen pressure of at or near 1 atm. In some embodiments, the hydrogenation is conducted at a hydrogen pressure of greater than 1 atm (e.g., about 2 atms, about 3 atms, about 5 atms, about 10 atms, about 15 atms, or any ranges between the recited values). In some embodiments, the hydrogenation is conducted at a hydrogen pressure of about 15 psi to about 100 psi (e.g., about 50 psi).
The compound of Formula I-8 can be obtained, for example, from reacting an aldehyde of Formula I-9 with an olefin forming reagent:
wherein Pg¹and Pg²are independently a hydroxyl protecting group as described hereinabove. Various olefin forming reagents are suitable for this transformation. Preferably, the reaction and work-up conditions are controlled such that no or minimal epimerization at the C20 position occurs. In some embodiments, the olefin forming reagent comprises a phosphonate substituted acetate:
wherein G¹at each occurrence and R^1aare each independently an optionally substituted alkyl group (e.g., an optionally substituted C_1-6alkyl, e.g., ethyl). The phosphonate substituted acetate can be deprotonated to form a phosphonate-stabilized carbanion, which reacts with the aldehyde group in Formula I-9 to form an alpha-beta-unsaturated ester. In some embodiments, the olefin forming reagent is ethyl 2-diethoxyphosphorylacetate. In some embodiments, the olefin forming reagent is a phosphorus ylide (e.g., triphenylcarbethoxymethylenephosphorane) or its precursor. In some embodiments, the olefin forming reagent can comprise an organometallic reagent, such as an organozinc reagent, which reacts with the aldehyde group in Formula I-9 to form a secondary alcohol, which upon dehydration, forms an alpha-beta-unsaturated ester. For example, in some embodiments, the olefin forming reagent comprises a two-carbon Reformatsky reagent:
wherein X is halo, such as Cl, Br or I, and R^1ais an optionally substituted alkyl group (e.g., an optionally substituted C_1-6alkyl, e.g., ethyl). In preferred embodiments, the ester group of the olefin forming reagent (e.g., R^1ain either of the phosphonate or the Reformatsky reagent) is the same as COOR¹and R¹is not H. However, in some embodiments, R^1ais different from R¹, for example, when R¹is H. In such embodiments, the method can optionally further include converting the —CO₂R^1ain Formula I-8b into —CO₂R¹in Formula I-8
The aldehyde of Formula I-9 can be formed from oxidizing the corresponding alcohol of Formula I-11:
wherein Pg¹and Pg²are independently a hydroxyl protecting group as described hereinabove. In some embodiments, the oxidizing comprises a Chromium trioxide or chromate based oxidation or variations thereof. In some embodiments, the oxidizing comprises a Swern oxidation or variations thereof. In some embodiments, the oxidizing comprises a hypervalent iodine mediated oxidation. In some embodiments, the oxidizing comprises contacting the compound of Formula I-11 with a suitable oxidizing agent. Various oxidizing agents are suitable. In some embodiments, the oxidizing agent is a Chromium based oxidizing agent, for example, PCC (pyridinium chlorochromate). In some embodiments, the oxidizing agent is a Swern oxidation reagent. Various Swern oxidation reagent systems are known. For example, a typical Swern oxidation can include using a DMSO/oxalyl chloride based system, in which DMSO first reacts with oxalyl chloride to generate a chloro(dimethyl)sulfonium chloride, which then converts an alcohol into an aldehyde or ketone group in the presence of an organic base (e.g., Et₃N). Other exemplary useful oxidizing agents include reagents for Swern oxidation or variations thereof which use cyanuric chloride, trifluoroacetic anhydride, carbodiimides, pyridine-sulfur trioxide complex to active DMSO, or use dimethyl sulfide and N-chlorosuccinimide to generate the chlorosulfonium active species. In some embodiments, the oxidizing agent can be 2-iodoxybenzoic acid (IBX) or Dess-Martin periodinane (1,1,1-Triacetoxy-1,1-dihydro-1,2-benziodoxol-3(1H)-one). Preferably, the reaction and work-up conditions are controlled such that no or minimal epimerization at the C20 position occurs.
Various methods can be employed for the synthesis of the compound of Formula I-11. In preferred embodiments, the compound of Formula I-11 can be synthesized from the compound of Formula I-10 or a geometric isomer thereof:
wherein Pg¹and Pg²are independently a hydroxyl protecting group as described hereinabove. In some embodiments, the method comprises reacting the compound of Formula I-10 with formaldehyde or paraformaldehyde in the presence of a Lewis acid. Various Lewis acids can be used for this transformation, for example, boron or aluminum based Lewis acids can be used. In some embodiments, the Lewis acid comprises BF₃. Other suitable Lewis acids include those known in the art that can facilitate an analogous reaction of an olefin with an aldehyde to provide an alcohol.
The compound of Formula I-10 used in the methods described hereinabove can be prepared via different methods. In any of the embodiments described herein, the compound of Formula I-10 can be prepared by the methods described herein, e.g., Method 1B below. In any of the embodiments described herein, the compound of Formula I-10 can also be a synthetic material free of any animal derived impurities.
Method 1B: Synthesis of 3,12-deoxygenated Steroid of Formula I-10
Certain embodiments of the present disclosure are directed to a method of preparing a compound of Formula I-10, or a geometric isomer thereof, wherein Pg¹and Pg²are independently a hydroxyl protecting group. In some embodiments, the method comprises obtaining the compound of Formula I-10 from DHEA or a protected DHEA (e.g., acetyl-DHEA) as exemplified in Scheme 3, wherein R², R³, Pg¹and Pg²are defined herein:
In some embodiments, the method comprises:
a) reacting a compound of Formula I-13
wherein R²is H or Pg¹; and Pg¹is a hydroxyl protecting group (e.g., as described herein); with an amine to form an imine of Formula I-14
wherein m is 0, 1 or 2, and R³is C_1-6alkyl (e.g., a methyl group at the 4′-position);
b) oxidizing the imine of Formula I-14 with O₂in the presence of a copper salt to form a compound of Formula I-15
c) reacting the compound of Formula I-15 with an olefin forming reagent to form a compound of Formula I-16, or a geometric isomer thereof,
and
d) protecting the hydroxyl group(s) in Formula I-16 to provide the compound of Formula I-10, or geometric isomer thereof.
In some embodiments, the protection of the 12-hydroxy group can occur prior to the olefination of the 17-ketone function in Formula I-15. Thus, in some embodiments, the method can also comprise:
c′) protecting the hydroxyl group(s) in the compound of Formula I-15 to form a compound of Formula I-15a
and
d′) then reacting the compound of Formula I-15a with an olefin forming reagent to provide the compound of Formula I-10, or geometric isomer thereof.
The imine formation of a), the copper mediated oxidation of b), and the olefination reaction of c) do not require the 3-hydroxy group to be protected. Thus, in some embodiments, the method can start with DHEA, i.e., R²is H. In some embodiments, the method can start with a protected version of DHEA, where R²is Pg¹, e.g., acetyl.
Various imines of Formula I-14 are suitable for the methods for preparing the compound of Formula I-10. In some embodiments, the pyridine ring in Formula I-14 is unsubstituted, i.e., m is 0. In some embodiments, the pyridine ring in Formula I-14 is substituted by one or two C1-6 alkyl groups. For example, in some embodiments, the pyridine ring in Formula I-14 is substituted at the 4′-position with a methyl group. The imine formation of a) is generally catalyzed by an acid, for example, para-toluenesulfonic acid.
Suitable copper salt for b) includes any of those known in the art for analogous transformations. In some embodiments, the copper salt is a copper (II) salt such as Cu(OTf)₂. In some embodiments, the copper salt is a copper (I) salt such as Cu(MeCN)₄PF₆.
In some embodiments, the olefin forming reagent in c) or d′) can be a phosphorous ylide. In some embodiments, the olefin forming reagent in c) or d′) can be ethylenetriphenylphosphorane or its precursor ethyltriphenylphosphonium salt. In some embodiments, the olefin forming reagent comprises a two-carbon organometallic reagent (e.g., an organozinc reagent), wherein the 17-ketone of Formula I-15 or Formula I-15a can be converted into an olefin by first forming an alcoholic intermediate with the organometallic reagent, which is then followed by dehydration.
Various protecting groups can be used for protecting either or both the 3- and 12-hydroxy groups. The protecting groups for the 3- and 12-hydroxy groups do not need to be the same. However, in preferred embodiments, Pg¹and Pg²are the same, for example, both Pg¹and Pg²are acetyl.
Method 2: Synthesis of Deoxycholic Acid from 3,12-deoxygenated Steroids
Deoxycholic acid (DCA) contains two hydroxyl groups at the 3,12-positions, which can therefore be prepared by an intermediate such as a compound of Formula I-1 (in the beta form with a 5-beta hydrogen, Formula I-1b) through a reduction reaction. In some embodiments, the present disclosure is directed to a method of preparing deoxycholic acid, an ester thereof, or a pharmaceutically or cosmetically acceptable salt thereof from a 3,12-deoxygenated steroid. In some embodiments, the method comprises a reaction sequence according to Scheme 4:
In some embodiments, the method of preparing deoxycholic acid, an ester thereof, or a pharmaceutically or cosmetically acceptable salt thereof comprises:
a) reacting a compound of Formula I-10, or a geometric isomer thereof, with formaldehyde or paraformaldehyde in the presence of a Lewis acid
to form a compound of Formula I-11
wherein Pg¹and Pg²are independently a hydroxyl protecting group;
b) oxidizing the compound of Formula I-11 to provide an aldehyde of Formula I-9
c) reacting the aldehyde of Formula I-9 with an olefin forming reagent to form a compound of Formula I-8, or a geometric isomer thereof,
wherein R¹is H or an optionally substituted alkyl group (e.g., an optionally substituted C_1-6alkyl);
d) hydrogenating the compound of Formula I-8 and subsequently removing the hydroxyl protecting groups, or deprotecting the compound of Formula I-8 and subsequently hydrogenating the deprotected compound, to provide a diol of Formula I-7b
e) oxidizing the diol of Formula I-7b to provide a compound of Formula I-1b,
f) contacting the compound of Formula I-1b with a ketone reducing agent to form a compound of Formula I-12
and
g) optionally, when R¹in Formula I-12 is not H, hydrolyzing the compound of Formula I-12 to provide deoxycholic acid.
The R¹group in Formula I-8, I-7b, I-1b, and I-12 can be the same or different. In some embodiments, R¹group in Formulae I-8, I-7b, I-1b, and I-12 can be the same, for example, as a C_1-6alkyl (e.g., methyl or ethyl).
In any of the embodiments described herein for Method 2, the compound of Formula I-1 can be prepared from the compound of Formula I-10 by any of the methods described herein, e.g., Method 1A. In any of the embodiments described herein, the compound of Formula I-10 can be prepared by any of the methods described herein, e.g., Method 1B.
Certain embodiments of the present disclosure are directed to a method of converting the compound of Formula I-1 into the compound of Formula I-12, for example, using a ketone reducing agent. In some embodiments, the ketone reducing agent is a borohydride (such as NaBH₄, NaCNBH₃, etc.) or a trialkoxyaluminum hydride (e.g., tri-isopropoxyaluminum hydride, tri-tert-butoxyaluminum hydride). In preferred embodiments, the ketone reducing agent is lithium tri-tert-butoxyaluminum hydride (LiAlH(O-tBu)₃).
When R¹is not hydrogen, for example, when R¹is a C_1-6alkyl, then to synthesize deoxycholic acid, the method further comprises hydrolyzing the compound of Formula I-12. Thus, in some embodiments, when R¹is not hydrogen, the method comprises hydrolyzing the compound of Formula I-12. Suitable hydrolyzing conditions include those known in the art. For example, the hydrolysis can be effected by reacting the compound of Formula I-12 under acid-mediated (e.g., BF₃mediated), base-mediated (e.g., using an alkali metal hydroxide (e.g., NaOH or LiOH)), or nucleophile-mediated (e.g., I⁻ mediated) hydrolysis conditions. In preferred embodiments, the hydrolysis comprises contacting the compound of Formula I-12 with an alkali metal hydroxide (e.g., NaOH or LiOH).
In some embodiments, an ester of deoxycholic acid, e.g., the compound of Formula I-12 wherein R¹is an optionally substituted alkyl, is the desired product. For example, in some embodiments, the method is directed to the synthesis of a pharmaceutically or cosmetically acceptable ester of deoxycholic acid. In such embodiments, the preferred reaction conditions for sequences a)-f) are those that no or minimal hydrolysis of the ester (i.e., —CO₂R¹) occurs under the respective reaction conditions. However, if desired, the esters of deoxycholic acid can also be prepared by esterification of deoxycholic acid with a desired alcohol.
In some embodiments, a salt of deoxycholic acid is the desired product. For example, in some embodiments, the method is directed to the synthesis of a pharmaceutically or cosmetically acceptable salt of deoxycholic acid. Thus, in some embodiments, the method comprises forming a pharmaceutically or cosmetically acceptable salt of deoxycholic acid, for example, by reacting deoxycholic acid with a suitable base, e.g., metal or ammonium hydroxide (e.g., sodium, potassium, lithium, calcium, magnesium, ammonium, or tetraalkylammonium hydroxide).
Novel Synthetic Intermediates from Methods 1, 1A, 1B and 2
Certain embodiments of the present disclosure are directed to novel synthetic intermediates useful for the preparation of cholic acid and/or deoxycholic acid. In some embodiments, the synthetic intermediate is a compound of Formula II-1, Formula II-2, Formula II-3, or Formula II-4, or a geometric isomer thereof, or a salt thereof:
wherein each of R¹⁰and R¹¹in each formula is independently H or a hydroxyl protecting group, and R¹²is H or an optionally substituted alkyl.
In some embodiments, R¹⁰and R¹¹are the same. In some embodiments, R¹⁰and R¹¹are different. In some embodiments, both R¹⁰and R¹¹are H. In some embodiments, one of R¹⁰and R¹¹is H and the other of R¹⁰and R¹¹is a hydroxyl protecting group (e.g., as described herein, e.g., acetyl). In some embodiments, both R¹⁰and R¹¹are hydroxyl protecting groups (e.g., as described herein, e.g., acetyl).
In some embodiments, R¹²is H. In some embodiments, R¹²is an optionally substituted alkyl (e.g., optionally substituted C_1-6alkyl). In some embodiments, R¹²is methyl. In some embodiments, R¹²is ethyl.
In some embodiments, the compound of Formula II-1, Formula II-2, Formula II-3, or Formula II-4 is isolated. In some embodiments, the isolated compound of Formula II-1, Formula II-2, Formula II-3, or Formula II-4 is substantially pure (e.g., at least 80%, at least 85%, at least 90%, at least 95%, or at least 98% pure by weight). In some embodiments, the isolated compound of Formula II-1, Formula II-2, Formula II-3, or Formula II-4 is about 80%, about 85%, about 90%, about 95%, about 98% pure by weight, or any ranges between the specified values. In some embodiments, the isolated compound of Formula II-1, Formula II-2, Formula II-3, or Formula II-4 is substantially free (e.g., less than 5%, less than 3%, less than 2%, less than 1%, less than 0.5%, or less than 0.1% by weight) of other diastereomers and/or geometric isomer. In some embodiments, the isolated compound of Formula II-1, Formula II-2, Formula II-3, or Formula II-4 has about 5%, about 3%, about 2%, about 1%, about 0.5%, about 0.1% by weight, or any ranges between the specified values, of other diastereomers and/or geometric isomer. In some embodiments, the isolated compound of Formula II-1, Formula II-2, Formula II-3, or Formula II-4 is free of (i.e., non-detectable using current analytical tools) other diastereomers and/or geometric isomer.
In some embodiments, the present disclosure also provides a compound of Formula II-5, Formula II-6, Formula II-7, or Formula II-8 having a structure of:
wherein R²⁰and R²³are each independently an optionally substituted alkyl, provided that R²⁰and R²³are not methyl; and
R²¹and R²²are each independently H or an optionally substituted alkyl,
or a salt thereof.
In some embodiments, R²⁰is an optionally substituted C2-6 alkyl. In some embodiments, R²⁰is a C2-4 alkyl, e.g., ethyl, isopropyl, etc.
In some embodiments, R²³is an optionally substituted C2-6 alkyl. In some embodiments, R²³is a C2-4 alkyl, e.g., ethyl, isopropyl, etc.
In some embodiments, R²¹is H. In some embodiments, R²¹is an optionally substituted alkyl, e.g., an optionally substituted C1-6 alkyl. In some embodiments, R²¹is a C2-4 alkyl, e.g., ethyl, isopropyl, etc.
In some embodiments, R²²is H. In some embodiments, R²²is an optionally substituted alkyl, e.g., an optionally substituted C1-6 alkyl. In some embodiments, R²²is a C2-4 alkyl, e.g., ethyl, isopropyl, etc.
In some embodiments, the compound of Formula II-5, Formula II-6, Formula II-7, or Formula II-8 is isolated. In some embodiments, the isolated compound of Formula II-5, Formula II-6, Formula II-7, or Formula II-8 is substantially pure (e.g., at least 80%, at least 85%, at least 90%, at least 95%, or at least 98% pure by weight). In some embodiments, the isolated compound of Formula II-5, Formula II-6, Formula II-7, or Formula II-8 is about 80%, about 85%, about 90%, about 95%, about 98% pure by weight, or any ranges between the specified values. In some embodiments, the isolated compound of Formula II-5, Formula II-6, Formula II-7, or Formula II-8 is substantially free (e.g., less than 5%, less than 3%, less than 2%, less than 1%, less than 0.5%, or less than 0.1% by weight) of other diastereomers and/or geometric isomer. In some embodiments, the isolated compound of Formula II-5, Formula II-6, Formula II-7, or Formula II-8 has about 5%, about 3%, about 2%, about 1%, about 0.5%, about 0.1% by weight, or any ranges between the specified values, of other diastereomers and/or geometric isomer. In some embodiments, the isolated compound of Formula II-5, Formula II-6, Formula II-7, or Formula II-8 is free of (i.e., non-detectable using current analytical tools) other diastereomers and/or geometric isomer.
The compound of any one of Formula II-1 to Formula II-8 can be prepared and purified according to the methods described herein, e.g., the Examples section.
Method 3: Synthesis of Cholic Acid from 3,7-deoxygenated or 3,7,12-trioxygenated Steroids
As described above, synthesis of cholic acid can also start with 3,7-deoxygenated or 3,7,12-trioxygenated steroids. In some embodiments, the 3,7-deoxygenated or 3,7,12-trioxygenated steroids can be obtained from a 3-hydroxy steroid starting material (e.g., DHEA) by first introducing a 7-hydroxy group followed by introducing a 12-hydroxy group. In various embodiments, the present disclosure also provides a method of preparing cholic acid, an ester thereof, or a pharmaceutically or cosmetically acceptable salt thereof, which comprises converting a 3,7-deoxygenated or 3,7,12-trioxygenated steroid into cholic acid, ester thereof, or a pharmaceutically or cosmetically acceptable salt thereof. For example, in some embodiments, the method comprises a reaction sequence shown in Scheme 5, wherein R³⁰, Pg¹⁰, Pg¹¹and Pg¹²are defined herein:
In some embodiments, the method of preparing cholic acid, an ester thereof, or a pharmaceutically or cosmetically acceptable salt thereof, comprises
a) reacting a compound of Formula III-1, or a geometric isomer thereof, with formaldehyde or paraformaldehyde in the presence of a Lewis acid to form a compound of Formula III-2
wherein Pg¹⁰, Pg¹¹and Pg¹²are each independently a hydroxyl protecting group;
b) oxidizing the compound of Formula III-2 to provide an aldehyde of Formula III-3
c) reacting the aldehyde of Formula III-3 with an olefin forming reagent to form a compound of Formula III-4, or a geometric isomer thereof,
wherein R³⁰is H or an optionally substituted alkyl group;
d) hydrogenating the compound of Formula III-4 with H₂gas in the presence of a metal catalyst and subsequently removing the hydroxyl protecting groups, or removing the hydroxyl protecting groups in Formula III-4 and subsequently hydrogenating the deprotected compound with H₂gas in the presence of a metal catalyst, to provide a triol of Formula III-5
e) oxidizing the triol of Formula III-5 to provide a compound of Formula III-6,
f) contacting the compound of Formula III-6 with a ketone reducing agent to form a compound of Formula III-7
and
g) optionally, when R³⁰is not H in Formula III-7, hydrolyzing the compound of Formula III-7 to provide cholic acid.
In some embodiments, R³⁰is hydrogen. However, for ease of purification, in preferred embodiments, R³⁰is an alkyl (e.g., methyl or ethyl). In some embodiments, R³⁰can also be an optionally substituted alkyl (e.g., an optionally substituted C_1-6alkyl). For example, R³⁰can be an alkyl optionally substituted by 1-3 (e.g., 1, 2, or 3) substituents, wherein the 1-3 substituents are independently a C_1-6alkyl, C_2-6alkenyl, C_2-6alkynyl, C_3-6carbocyclyl, 3-6 membered heterocyclyl, 5 or 6 membered heteroaryl, phenyl, a substituted phenyl (e.g., para-methoxyphenyl), hydroxyl, C_1-6alkoxy (e.g., a methoxy, ethoxy, etc.), halogen (e.g., F or Cl), or amino optionally substituted by one or two groups independently selected from C_1-6alkyl. In preferred embodiments, R³⁰is a C_1-6alkyl (e.g., methyl or ethyl). The R³⁰group in Formula III-4 to III-7 can be the same or different. In some embodiments, R³⁰group in Formula III-4 to III-7 can be the same, for example, as a C_1-6alkyl (e.g., methyl or ethyl).
Pg¹⁰, Pg¹¹and Pg¹²are each independently a hydroxyl protecting group (e.g., as described herein). In some embodiments, Pg¹⁰, Pg¹¹and Pg¹²are each acetyl.
Various conditions for effecting the transformations according to Scheme 5 or a), b), c), d), e), f) and g) described above can be used, which are exemplified in embodiments below and in the Examples section. Other useful conditions and details include those known in the art for analogous transformations.
Certain embodiments of the present disclosure are directed to the method of converting the compound of Formula III-1 or a geometric isomer thereof, into the compound of Formula III-2. In some embodiments, the method comprises reacting a compound of Formula III-1 with formaldehyde or paraformaldehyde in the presence of a Lewis acid. Various Lewis acids can be used for this transformation, for example, boron or aluminum based Lewis acids can be used. In some embodiments, the Lewis acid comprises BF₃. Other suitable Lewis acids include those known in the art that can facilitate an analogous reaction of an olefin with an aldehyde to provide an alcohol.
Certain embodiments of the present disclosure are directed to the method of oxidizing the compound of Formula III-2 into the compound of Formula III-3. In some embodiments, the oxidizing comprises a Chromium trioxide or chromate based oxidation or variations thereof. In some embodiments, the oxidizing comprises a Swern oxidation or variations thereof. In some embodiments, the oxidizing comprises a hypervalent iodine mediated oxidation. In some embodiments, the oxidizing comprises contacting the compound of Formula I-11 with a suitable oxidizing agent. Various oxidizing agents are suitable. In some embodiments, the oxidizing agent is a Chromium based oxidizing agent, for example, PCC (pyridinium chlorochromate). In some embodiments, the oxidizing agent is a Swern oxidation reagent. Various Swern oxidation reagent systems are known. For example, a typical Swern oxidation can include using a DMSO/oxalyl chloride based system, in which DMSO first reacts with oxalyl chloride to generate a chloro(dimethyl)sulfonium chloride, which then converts an alcohol into an aldehyde or ketone group in the presence of an organic base (e.g., Et₃N). Other exemplary useful oxidizing agents include reagents for Swern oxidation or variations thereof which use cyanuric chloride, trifluoroacetic anhydride, carbodiimides, pyridine-sulfur trioxide complex to active DMSO, or use dimethyl sulfide and N-chlorosuccinimide to generate the chlorosulfonium active species. In some embodiments, the oxidizing agent can be 2-iodoxybenzoic acid (IBX) or Dess-Martin periodinane (1,1,1-Triacetoxy-1,1-dihydro-1,2-benziodoxol-3(1H)-one). Preferably, the reaction and work-up conditions are controlled such that no or minimal epimerization at the C20 position occurs.
Certain embodiments of the present disclosure are directed to the method of converting the compound of Formula III-3 into the compound of Formula III-4 using an olefin forming reagent. Preferably, the reaction and work-up conditions are controlled such that no or minimal epimerization at the C20 position occurs. In some embodiments, the olefin forming reagent comprises a phosphonate substituted acetate:
wherein G¹⁰at each occurrence and R^30aare each independently an optionally substituted alkyl group (e.g., an optionally substituted C_1-6alkyl, e.g., ethyl). The phosphonate substituted acetate can be deprotonated to form a phosphonate-stabilized carbanion, which reacts with the aldehyde group in Formula III-3 to form an alpha-beta-unsaturated ester. In some embodiments, the olefin forming reagent is ethyl 2-diethoxyphosphorylacetate. In some embodiments, the olefin forming reagent is a phosphorus ylide (e.g., triphenylcarbethoxymethylenephosphorane) or its precursor. In some embodiments, the olefin forming reagent can comprise an organometallic reagent, such as an organozinc reagent, which reacts with the aldehyde group in Formula III-3 to form a secondary alcohol, which upon dehydration, forms an alpha-beta-unsaturated ester. For example, in some embodiments, the olefin forming reagent comprises a two-carbon Reformatsky reagent:
wherein X is halo, such as Cl, Br or I, and R^30ais an optionally substituted alkyl group (e.g., an optionally substituted C_1-6alkyl, e.g., ethyl). In preferred embodiments, the ester group of the olefin forming reagent (e.g., R^30ain either of the phosphonate or the Reformatsky reagent) is the same as COOR³⁰and R³⁰is not H. However, in some embodiments, R^30ais different from R³⁰, for example, when R³⁰is H. In such embodiments, the method can optionally further include converting the —CO₂R^30ain Formula III-4a into —CO₂R³⁰in Formula III-4
Certain embodiments of the present disclosure are directed to the method of converting the compound of Formula III-4 into the compound of Formula III-5. In some embodiments, the method comprises hydrogenating the compound of Formula III-4 with H₂gas in the presence of a metal catalyst and subsequently removing the hydroxyl protecting groups. In some embodiments, the method comprises removing the hydroxyl protecting groups in Formula III-4 to provide a triol analog and subsequently hydrogenating the deprotected triol analog with H₂gas in the presence of a metal catalyst.
As the olefin double bonds in Formula III-4 or the triol analog of Formula III-4 are hydrogenated during the above transformation to form the triol of Formula III-5, the geometric isomers of the compounds of Formula III-4 or the triol analog of Formula III-4 can also be used. In some embodiments, the method comprises hydrogenating a compound of Formula III-4 or the triol analog of Formula III-4. In some embodiments, the method comprises hydrogenating a geometric isomer of the compound of Formula III-4 or the triol analog of Formula III-4. In some embodiments, the method comprises hydrogenating a mixture of the compound of Formula III-4 or the triol analog of Formula III-4, and its geometric isomers.
Various methods for hydrogenating the compound of Formula III-4 or the triol analog of Formula III-4 can be used. Preferably, the method comprises hydrogenating the compound of Formula III-4 or the triol analog of Formula III-4 with H₂in the presence of a heterogeneous catalyst, for example, a heterogeneous metal catalyst comprising Pd, Ni, Pt, PtO₂, Rh, or Ru. In some embodiments, the heterogeneous metal catalyst is Pd/C. The hydrogenation can be conducted at various H₂pressures. In some embodiments, the hydrogenation is conducted at a hydrogen pressure of at or near 1 atm. In some embodiments, the hydrogenation is conducted at a hydrogen pressure of greater than 1 atm (e.g., about 2 atms, about 3 atms, about 5 atms, about 10 atms, about 15 atms, or any ranges between the recited values). In some embodiments, the hydrogenation is conducted at a hydrogen pressure of about 15 psi to about 100 psi (e.g., about 50 psi).
In some embodiments, the compound of Formula III-4 is converted into Formula III-5a by a process comprising hydrogenation, deprotection, and ester hydrolysis, regardless of sequence:
In such embodiments, the method comprises converting Formula III-5a into cholic acid, an ester thereof, or a pharmaceutically or cosmetically acceptable salt thereof, for example, according to the reaction sequence in Scheme 5a:
Suitable conditions for the transformations in Scheme 5a include those described herein, for example, in the Examples section.
Certain embodiments of the present disclosure are directed to the method of oxidizing the compound of Formula III-5 into the compound of Formula III-6. In some embodiments, the oxidizing comprises a Chromium trioxide or chromate based oxidation or variations thereof. In some embodiments, the oxidizing comprises a Swern oxidation or variations thereof. In some embodiments, the oxidizing comprises a hypervalent iodine mediated oxidation. In some embodiments, the oxidizing comprises contacting the triol of Formula III-5 with a suitable oxidizing agent. In some embodiments, the oxidizing agent is a Chromium based oxidizing agent, for example, Jones reagent (a solution of chromium trioxide in dilute sulfuric acid); PCC (pyridinium chlorochromate), etc. In a preferred embodiment, the oxidizing agent is Jones reagent. In some embodiments, the oxidizing agent is a Swern oxidation reagent. Various Swern oxidation reagent systems are known. For example, a typical Swern oxidation can include using a DMSO/oxalyl chloride based system, in which DMSO first reacts with oxalyl chloride to generate a chloro(dimethyl)sulfonium chloride, which then converts an alcohol into an aldehyde or ketone group in the presence of an organic base (e.g., Et₃N). Other exemplary useful oxidizing agents include reagents for Swern oxidation or variations thereof which use cyanuric chloride, trifluoroacetic anhydride, carbodiimides, pyridine-sulfur trioxide complex to active DMSO, or use dimethyl sulfide and N-chlorosuccinimide to generate the chlorosulfonium active species. In some embodiments, the oxidizing agent can be 2-iodoxybenzoic acid (IBX) or Dess-Martin periodinane (1,1,1-Triacetoxy-1,1-dihydro-1,2-benziodoxol-3(1H)-one).
Certain embodiments of the present disclosure are directed to the method of converting the compound of Formula III-6 into the compound of Formula III-7, for example, using a ketone reducing agent. In some embodiments, the ketone reducing agent is a borohydride (such as NaBH₄, NaCNBH₃, etc.) or a trialkoxyaluminum hydride (e.g., tri-isopropoxyaluminum hydride, tri-tert-butoxyaluminum hydride). In preferred embodiments, the ketone reducing agent is lithium tri-tert-butoxyaluminum hydride (LiAlH(O-tBu)₃).
When R³⁰is not hydrogen, for example, when R³⁰is a C_1-6alkyl, then to synthesize cholic acid, the method further comprises hydrolyzing the compound of Formula III-7. Thus, in some embodiments, when R³⁰is not hydrogen, the method comprises hydrolyzing the compound of Formula III-7. Suitable hydrolyzing conditions include those known in the art. For example, the hydrolysis can be effected by reacting the compound of Formula III-7 under acid-mediated (e.g., BF₃mediated), base-mediated (e.g., using an alkali metal hydroxide (e.g., NaOH or LiOH)), or nucleophile-mediated (e.g., I⁻ mediated) hydrolysis conditions. In preferred embodiments, the hydrolysis comprises contacting the compound of Formula III-7 with an alkali metal hydroxide (e.g., NaOH or LiOH).
In some embodiments, an ester of cholic acid, e.g., the compound of Formula III-7 wherein R³⁰is an optionally substituted alkyl, is the desired product. For example, in some embodiments, the method is directed to the synthesis of a pharmaceutically or cosmetically acceptable ester of cholic acid. In such embodiments, the preferred reaction conditions for sequences a)-f) are those that no or minimal hydrolysis of the ester (i.e., —CO₂R³⁰) occurs under the respective reaction conditions. However, if desired, the esters of cholic acid can also be prepared by esterification of cholic acid with a desired alcohol.
In some embodiments, a salt of cholic acid is the desired product. For example, in some embodiments, the method is directed to the synthesis of a pharmaceutically or cosmetically acceptable salt of cholic acid. Thus, in some embodiments, the method comprises forming a pharmaceutically or cosmetically acceptable salt of cholic acid, for example, by reacting cholic acid with a suitable base, e.g., metal or ammonium hydroxide (e.g., sodium, potassium, lithium, calcium, magnesium, ammonium, or tetraalkylammonium hydroxide).
The compound of Formula III-1 or geometric isomer thereof used in the methods described hereinabove can be prepared via different methods. For example, in some embodiments, the compound of Formula III-1 or geometric isomer thereof can be synthesized from reacting a compound of Formula III-8 with an olefin forming reagent:
wherein R³¹is H or Pg¹⁰; R³²is H or Pg¹¹; and R³³is H or Pg¹²; provided that when one or more of R³¹, R³²and R³³are H, then a protecting step is carried out after reacting the compound of Formula III-8 with the olefin forming reagent. However, in some embodiments, when one or more of R³¹, R³²and R³³are H, the protecting step can also be carried out first before reacting with the olefin forming reagent. Various olefin forming reagents are suitable for the transformation. In some embodiments, the olefin forming reagent can be a phosphorous ylide. In some embodiments, the olefin forming reagent can be ethylenetriphenylphosphorane or its precursor ethyltriphenylphosphonium salt. In some embodiments, the olefin forming reagent comprises a two-carbon organometallic reagent (e.g., an organozinc reagent), wherein the 17-ketone of Formula III-8 can be converted into an olefin by first forming an alcoholic intermediate with the organometallic reagent, which is then followed by dehydration.
In some embodiments, R³¹, R³²and R³³are all H (a compound of Formula III-8a) and the protecting step can be carried out before or after reacting with the olefin forming reagent:
The compound of Formula III-8a can also be served as starting material for the synthesis of the compound of Formula III-8, where at least one of R³¹, R³²and R³³are not H. For example, in some embodiments, R³¹, R³²and R³³are each acetyl, the compound of Formula III-8 can be prepared by reacting the compound of Formula III-8a with a suitable acetyl donor (e.g., acetic anhydride).
In any of the embodiments described herein, the compound of Formula III-8a can be prepared by the methods described herein, e.g., Method 3A below. In any of the embodiments described herein, the compound of Formula III-8a can also be a synthetic material free of any animal derived impurities.
Method 3A: Synthesis of the Compound of Formula III-8a from DHEA via 3,7-deoxygenated Steroids
Certain embodiments of the present disclosure are directed to a method of preparing a compound of Formula III-8a. In some embodiments, the method comprises obtaining the compound of Formula III-8a from DHEA as exemplified in Scheme 6:
wherein n is 0, 1 or 2, and R³⁴is C_1-6alkyl.
In some embodiments, the method comprises:
a) oxidizing dehydroepiandrosterone (DHEA) into a compound of Formula III-9
b) reacting the compound of Formula III-9 with an epoxide forming reagent to form an epoxide of Formula III-10
c) reducing the epoxide of Formula III-10 under hydrogenation condition to form a diketone of Formula III-11
d) contacting the diketone of Formula III-11 with a ketone reducing agent to form a compound of Formula III-12
e) reacting the compound of Formula III-12 with an amine to form an imine of Formula III-13
wherein n is 0, 1 or 2, and R³⁴is C_1-6alkyl; and
f) oxidizing the imine of Formula III-13 with O₂in the presence of a copper salt to form the compound of Formula III-8a.
Various conditions are suitable for the transformations described in scheme 6 or a)-f) above, which are exemplified in the embodiments below and in the Examples section. Other useful conditions and details include those known in the art for analogous transformations.
For example, DHEA can be converted into the compound of Formula III-9 through oxidation. In some embodiments, the oxidation can be carried out with a benzoquinone based oxidizing agent (e.g., DDQ or chloranil) and an amine oxide (e.g., TEMPO (2,2,6,6-tetramethylpiperidin-1-yl)oxidanyl)).
The compound of Formula III-9 can be converted into the compound of Formula III-10 using an epoxide forming reagent. In some embodiments, the epoxide forming reagent is a peroxycarboxylic acid. In some embodiments, the epoxide forming reagent is meta-chloroperoxybenzoic acid. Other suitable epoxide forming reagents include those known in the art that can selectively produce an epoxide at the 6,7 positions with the olefin at the 4,5 positions intact.
In some embodiments, conversion of the compound of Formula III-10 into the compound of Formula III-11 can be achieved through a hydrogenation reaction. For example, in some embodiments, the method comprises hydrogenating the compound of Formula III-10 with H₂under the catalysis of a heterogeneous catalyst, for example, a heterogeneous metal catalyst comprising Pd, Ni, Pt, PtO₂, Rh, or Ru. In some embodiments, the heterogeneous metal catalyst is Pd/C. The hydrogenation can be conducted at various H₂pressures. In some embodiments, the hydrogenation is conducted at a hydrogen pressure of at or near 1 atm. In some embodiments, the hydrogenation is conducted at a hydrogen pressure of greater than 1 atm (e.g., about 2 atms, about 3 atms, about 5 atms, about 10 atms, about 15 atms, or any ranges between the recited values). In some embodiments, the hydrogenation is conducted at a hydrogen pressure of about 15 psi to about 100 psi (e.g., about 50 psi).
In some embodiments, conversion of the compound of Formula III-11 into the compound of Formula III-12 can be achieved through a reduction reaction. Various ketone reducing agents can be used to effect this transformation. In some embodiments, the ketone reducing agent is a borohydride (such as NaBH₄, NaCNBH₃, etc.) or a trialkoxyaluminum hydride (e.g., tri-isopropoxyaluminum hydride, tri-tert-butoxyaluminum hydride). In preferred embodiments, the ketone reducing agent is lithium tri-tert-butoxyaluminum hydride (LiAlH(O-tBu)₃).
In some embodiments, the 3,7-dihydroxy compound of Formula III-12 can be converted into the imine of Formula III-13 with a corresponding amine. Various imines of Formula III-13 are suitable for the methods described herein. In some embodiments, the pyridine ring in Formula III-13 is unsubstituted, i.e., n is 0. In some embodiments, the pyridine ring in Formula III-13 is substituted by one or two C1-6 alkyl groups. For example, in some embodiments, the pyridine ring in Formula III-13 is substituted at the 4′-position by a methyl group. The imine formation is generally catalyzed by an acid, for example, para-toluenesulfonic acid.
Suitable copper salt for the transformation of the compound of Formula III-13 into the compound of Formula III-8a includes any of those known in the art for analogous transformations. In some embodiments, the copper salt is a copper (II) salt such as Cu(OTf)₂. In some embodiments, the copper salt is a copper (I) salt such as Cu(MeCN)₄PF₆.
The reduction of the compound of Formula III-11, the imine formation of the compound of Formula III-12, and the copper mediated oxidation of the imine of Formula III-13 do not require the hydroxyl group to be protected or to be a free hydroxyl group. Thus, in some embodiments, one or more of Formula III-11, Formula III-12, and Formula III-13 can be protected and used for the synthesis of either the compound of Formula III-8a or Formula III-8, with appropriate protection or deprotection depending on the target product. In some embodiments, the present disclosure provides a method of preparing a compound of Formula III-8 according to scheme 7, wherein R³¹, R³², R³³, and R³⁴are as described hereinabove:
The transformations described in scheme 7 are similar to those described in Scheme 6, except that R³³in Formula III-11a, Formula III-12a, and Formula III-13a, and R³¹in Formula III-12a, and Formula III-13a, can be hydrogen or a hydroxyl protecting group as described herein.
Method 4: Synthesis of Chenodeoxycholic Acid from 3,7-deoxygenated Steroids
Chenodeoxycholic acid (CDCA) contains two hydroxyl groups at the 3,7-positions, which can be prepared by an intermediate such as a compound of Formula III-12 or a hydroxyl-protected derivative thereof. In some embodiments, the present disclosure is directed to a method of preparing chenodeoxycholic acid, an ester thereof, or a pharmaceutically or cosmetically acceptable salt thereof from a 3,7-deoxygenated steroid. In some embodiments, the method comprises a reaction sequence according to Scheme 8, wherein Pg²⁰and Pg²¹are each independently a hydroxyl protecting group and R⁴⁰is H or an optionally substituted alkyl group:
In some embodiments, the method comprises:
a) reacting a compound of Formula IV-1, or a geometric isomer thereof, with formaldehyde or paraformaldehyde in the presence of a Lewis acid to form a compound of Formula IV-2
wherein Pg²⁰and Pg²¹are each independently a hydroxyl protecting group;
b) oxidizing the compound of Formula IV-2 to provide an aldehyde of Formula IV-3
c) reacting the aldehyde of Formula IV-3 with an olefin forming reagent to form a compound of Formula IV-4, or a geometric isomer thereof,
wherein R⁴⁰is H or an optionally substituted alkyl group;
d) hydrogenating the compound of Formula IV-4 with H₂gas in the presence of a metal catalyst and subsequently removing the hydroxyl protecting groups, or removing the hydroxyl protecting groups of Formula IV-4 and subsequently hydrogenating the deprotected compound with H₂gas in the presence of a metal catalyst, to provide a diol of Formula IV-5
e) optionally, when R⁴⁰is not H in Formula IV-5, hydrolyzing the compound of Formula IV-5 into chenodeoxycholic acid.
Suitable hydroxyl protecting groups for Pg²⁰and Pg²¹include any of those known in the art, for example, those described herein. In some embodiments, Pg²⁰and Pg²¹are the same. In some embodiments, Pg²⁰and Pg²¹are different. In preferred embodiments, both Pg²⁰and Pg²¹are hydroxyl protecting group that forms an ester with the hydroxyl group, for example, both Pg²⁰and Pg²¹are acetyl.
In some embodiments, R⁴⁰is hydrogen. However, for ease of purification, in preferred embodiments, R⁴⁰is a C_1-6alkyl (e.g., methyl or ethyl). In some embodiments, R⁴⁰can also be an optionally substituted alkyl (e.g., an optionally substituted C_1-6alkyl). For example, R⁴⁰can be an alkyl optionally substituted by 1-3 (e.g., 1, 2, or 3) substituents, wherein the 1-3 substituents are independently a C_1-6alkyl, C_2-6alkenyl, C_2-6alkynyl, C_3-6carbocyclyl, 3-6 membered heterocyclyl, 5 or 6 membered heteroaryl, phenyl, a substituted phenyl (e.g., para-methoxyphenyl), hydroxyl, C_1-6alkoxy (e.g., a methoxy, ethoxy, etc.), halogen (e.g., F or Cl), or amino optionally substituted by one or two groups independently selected from C_1-6alkyl. In preferred embodiments, R⁴⁰is a C_1-6alkyl (e.g., methyl or ethyl). R⁴⁰group in Formulae IV-4 and IV-5 can be the same or different. In some embodiments, R⁴⁰group in Formulae IV-4 and IV-5 can be the same, e.g., as a C_1-6alkyl (e.g., methyl or ethyl).
Various conditions for effecting the transformations in Scheme 8 and a), b), c), d) and e) in Method 4 can be used, which are exemplified in the embodiments below and in the Examples section. Other useful conditions and details include those known in the art for analogous transformations.
Certain embodiments of the present disclosure are directed to a method of converting the compound of Formula IV-1 into the compound of Formula IV-2. In some embodiments, the conversion comprises reacting a compound of Formula IV-1 or a geometric isomer thereof, with formaldehyde or paraformaldehyde in the presence of a Lewis acid. Various Lewis acids can be used for this transformation, for example, boron or aluminum based Lewis acids can be used. In some embodiments, the Lewis acid comprises BF₃. Other suitable Lewis acids include those known in the art that can facilitate an analogous reaction of an olefin with an aldehyde to provide an alcohol.
Certain embodiments of the present disclosure are directed to a method of oxidizing the compound of Formula IV-2 into the compound of Formula IV-3 by using an oxidizing agent. In some embodiments, the oxidizing comprises a Chromium trioxide or chromate based oxidation or variations thereof. In some embodiments, the oxidizing comprises a Swern oxidation or variations thereof. In some embodiments, the oxidizing comprises a hypervalent iodine mediated oxidation. In some embodiments, the oxidizing comprises contacting the compound of Formula IV-2 with a suitable oxidizing agent. In some embodiments, the oxidizing agent is a Chromium based oxidizing agent, for example, PCC (pyridinium chlorochromate). In some embodiments, the oxidizing agent is a Swern oxidation reagent. Various Swern oxidation reagent systems are known. For example, a typical Swern oxidation can include using a DMSO/oxalyl chloride based system, in which DMSO first reacts with oxalyl chloride to generate a chloro(dimethyl)sulfonium chloride, which then converts an alcohol into an aldehyde or ketone group in the presence of an organic base (e.g., Et₃N). Other exemplary useful oxidizing agents include reagents for Swern oxidation or variations thereof, which use cyanuric chloride, trifluoroacetic anhydride, carbodiimides, pyridine-sulfur trioxide complex to active DMSO, or use dimethyl sulfide and N-chlorosuccinimide to generate the chlorosulfonium active species. In some embodiments, the oxidizing agent can be 2-iodoxybenzoic acid (IBX) or Dess-Martin periodinane (1,1,1-Triacetoxy-1,1-dihydro-1,2-benziodoxol-3(1H)-one). Preferably, the reaction and work-up conditions are controlled such that no or minimal epimerization at the C20 position occurs.
Certain embodiments of the present disclosure are directed to a method of converting the compound of Formula IV-3 into the compound of Formula IV-4 by using an olefin forming reagent. Various olefin forming reagents are suitable for this transformation. Preferably, the reaction and work-up conditions are controlled such that no or minimal epimerization at the C20 position occurs. In some embodiments, the olefin forming reagent comprises a phosphonate substituted acetate:
wherein G⁴⁰at each occurrence and R^40aare each independently an optionally substituted alkyl group (e.g., an optionally substituted C_1-6alkyl, e.g., ethyl). The phosphonate substituted acetate can be deprotonated to form a phosphonate-stabilized carbanion, which reacts with the aldehyde group in Formula IV-3 to form an alpha-beta-unsaturated ester. In some embodiments, the olefin forming reagent is ethyl 2-diethoxyphosphorylacetate. In some embodiments, the olefin forming reagent is a phosphorus ylide (e.g., triphenylcarbethoxymethylenephosphorane) or its precursor. In some embodiments, the olefin forming reagent can comprise an organometallic reagent, such as an organozinc reagent, which reacts with the aldehyde group in Formula IV-3 to form a secondary alcohol, which upon dehydration, forms an alpha-beta-unsaturated ester. For example, in some embodiments, the olefin forming reagent comprises a two-carbon Reformatsky reagent:
wherein X is halo, such as Cl, Br or I, and R^40ais an optionally substituted alkyl group (e.g., an optionally substituted C_1-6alkyl, e.g., ethyl). In preferred embodiments, the ester group of the olefin forming reagent (e.g., R^40ain either of the phosphonate or the Reformatsky reagent) is the same as COOR⁴⁰and R⁴⁰is not H. However, in some embodiments, R^40ais different from R⁴⁰, for example, when R⁴⁰is H. In such embodiments, the method can optionally further include converting the —CO₂R^40ain Formula IV-4a into —CO₂R⁴⁰in Formula IV-4
Certain embodiments of the present disclosure are directed to a method of converting the compound of Formula IV-4 into the compound of Formula IV-5. In some embodiments, the diol of Formula IV-5 is synthesized by hydrogenating the compound of Formula IV-4 followed by removing the hydroxyl protecting groups. In some embodiments, the diol of Formula IV-5 is synthesized by removing the hydroxyl protecting groups of the compound of Formula IV-4 followed by hydrogenation of the double bonds. As the olefin double bonds in Formula IV-4 are hydrogenated during the above transformation to form the diol of Formula IV-5, the geometric isomers of the compounds of Formula IV-4 can also be used. In some embodiments, the method comprises hydrogenating the compound of Formula IV-4. In some embodiments, the method comprises hydrogenating a geometric isomer of the compound of Formula IV-4. In some embodiments, the method comprises hydrogenating a mixture of the compound of Formula IV-4, and its geometric isomers.
Various methods for hydrogenating the compound of Formula IV-4 can be used. Preferably, the method comprises hydrogenating the compound of Formula IV-4 with H₂in the presence of a heterogeneous catalyst, for example, a heterogeneous metal catalyst comprising Pd, Ni, Pt, PtO₂, Rh, or Ru. In some embodiments, the heterogeneous metal catalyst is Pd/C. The hydrogenation can be conducted at various H₂pressures. In some embodiments, the hydrogenation is conducted at a hydrogen pressure of at or near 1 atm. In some embodiments, the hydrogenation is conducted at a hydrogen pressure of greater than 1 atm (e.g., about 2 atms, about 3 atms, about 5 atms, about 10 atms, about 15 atms, or any ranges between the recited values). In some embodiments, the hydrogenation is conducted at a hydrogen pressure of about 15 psi to about 100 psi (e.g., about 50 psi).
Various methods can be used for the synthesis of the compound of Formula IV-1 or a geometric isomer thereof. In some embodiments, the compound of Formula IV-1 or a geometric isomer thereof is obtained from reacting a compound of Formula IV-6 with an olefin forming reagent
wherein R⁴¹is H or Pg²⁰; R⁴²is H or Pg²¹;
provided that when one or both of R⁴¹and R⁴²are H, then a protecting step is followed after reacting the compound of Formula IV-6 with the olefin forming reagent. In some embodiments, the olefin forming reagent can be a phosphorous ylide. In some embodiments, the olefin forming reagent can be ethylenetriphenylphosphorane or its precursor ethyltriphenylphosphonium salt. In some embodiments, the olefin forming reagent comprises a two-carbon organometallic reagent (e.g., an organozinc reagent), wherein the 17-ketone of Formula IV-6 can be converted into an olefin by first forming an alcoholic intermediate with the organometallic reagent, which is then followed by dehydration.
When R⁴⁰is not hydrogen, for example, when R⁴⁰is a C_1-6alkyl, then to synthesize chenodeoxycholic acid, the method further comprises hydrolyzing the compound of Formula IV-5. Thus, in some embodiments, when R⁴⁰is not hydrogen, the method comprises hydrolyzing the compound of Formula IV-5. Suitable hydrolyzing conditions include those known in the art. For example, the hydrolysis can be effected by reacting the compound of Formula IV-5 under acid-mediated (e.g., BF₃mediated), base-mediated (e.g., using an alkali metal hydroxide (e.g., NaOH or LiOH)), or nucleophile-mediated (e.g., I⁻ mediated) hydrolysis conditions. In preferred embodiments, the hydrolysis comprises contacting the compound of Formula IV-5 with an alkali metal hydroxide (e.g., NaOH or LiOH).
In some embodiments, an ester of chenodeoxycholic acid, e.g., the compound of Formula IV-5, wherein R⁴⁰is an optionally substituted alkyl, is the desired product. For example, in some embodiments, the method is directed to the synthesis of a pharmaceutically or cosmetically acceptable ester of chenodeoxycholic acid. In such embodiments, the preferred reaction conditions for sequences a)-d) are those that no or minimal hydrolysis of the ester (i.e., —CO₂R⁴⁰) occurs under the respective reaction conditions. However, if desired, the esters of chenodeoxycholic acid can also be prepared by esterification of chenodeoxycholic acid with a desired alcohol.
In some embodiments, a salt of chenodeoxycholic acid is the desired product. For example, in some embodiments, the method is directed to the synthesis of a pharmaceutically or cosmetically acceptable salt of chenodeoxycholic acid. Thus, in some embodiments, the method comprises forming a pharmaceutically or cosmetically acceptable salt of chenodeoxycholic acid, for example, by reacting chenodeoxycholic acid with a suitable base, e.g., metal or ammonium hydroxide (e.g., sodium, potassium, lithium, calcium, magnesium, ammonium, or tetraalkylammonium hydroxide).
Compounds of Formula IV-6 can be readily available from either Formula III-11 or Formula III-12. For example, when both R⁴¹and R⁴²are H, Formula IV-6 is the same as Formula III-12. When both R⁴¹and R⁴²are the same hydroxyl protecting group, a double protection of Formula III-12 with an appropriate protecting group can provide the compound of Formula IV-6. Further, when R⁴¹and R⁴²are different, the compound of Formula IV-6 can be provided through the compound of Formula III-11 by using a combination of protection/deprotection and reduction steps, so as to differentiate the two 3- and 7-hydroxyl groups regardless of their intrinsic reactivity.
In any of the embodiments described herein for Method 4, the compound of Formula IV-6 can be prepared from the compound of Formula III-11 or Formula III-12, which in turn can be prepared by any of the methods described herein, e.g., Method 3. In any of the embodiments described herein, the compound of Formula III-11 or Formula III-12 can also be a synthetic material free of any animal derived impurities.
Additionally, as will be apparent to those skilled in the art, conventional protecting groups may be necessary to prevent certain functional groups from undergoing undesired reactions. Suitable protecting groups for various functional groups as well as suitable conditions for protecting and deprotecting particular functional groups are well known in the art. For example, numerous protecting groups are described in “Protective Groups in Organic Synthesis”, 4^thed. P. G. M. Wuts; T. W. Greene, John Wiley, 2007, and references cited therein. The reagents for the reactions described herein are generally known compounds or can be prepared by known procedures or obvious modifications thereof. For example, many of the reagents are available from commercial suppliers such as Aldrich Chemical Co. (Milwaukee, Wis., USA), Bachem (Torrance, Calif., USA), Emka-Chem or Sigma (St. Louis, Mo., USA). Others may be prepared by procedures, or obvious modifications thereof, described in standard reference texts such as Fieser and Fieser's Reagents for Organic Synthesis, Volumes 1-15 (John Wiley and Sons, 1991), Rodd's Chemistry of Carbon Compounds, Volumes 1-5 and Supplemental (Elsevier Science Publishers, 1989), Organic Reactions, Volumes 1-40 (John Wiley and Sons, 1991), March's Advanced Organic Chemistry, (Wiley, 7^thEdition), and Larock's Comprehensive Organic Transformations (Wiley-VCH, 1999).
Novel Synthetic Intermediates from Methods 3 and 4
Certain embodiments of the present disclosure are directed to useful novel synthetic intermediates, for example, for the preparation of cholic acid and/or chenodeoxycholic acid. In some embodiments, the synthetic intermediate is a compound of Formula V-1, Formula V-2, Formula V-3, or Formula V-4, or a geometric isomer thereof, or a salt thereof:
wherein each of R⁵⁰, R⁵¹, and R⁵²is independently H or a hydroxyl protecting group, and R⁵³is H or an optionally substituted alkyl.
R⁵⁰, R⁵¹, and R⁵²in each of Formula V-1, Formula V-2, Formula V-3, and Formula V-4 can be independently selected. In some embodiments, R⁵⁰, R⁵¹, and R⁵²are each H. In some embodiments, R⁵⁰, R⁵¹, and R⁵²are each independently a hydroxyl protecting group (e.g., as described herein). In some embodiments, R⁵⁰, R⁵¹, and R⁵²are the same hydroxyl protecting group. In some embodiments, at least two of R⁵⁰, R⁵¹, and R⁵²are the same hydroxyl protecting group. In some embodiments, at least two of R⁵⁰, R⁵¹, and R⁵²are different. For example, one of R⁵⁰, R⁵¹, and R⁵²can be hydrogen and the other two of R⁵⁰, R⁵¹, and R⁵²can be the same or different hydroxyl protecting group. Suitable hydroxyl protecting groups include any of those described herein. In some embodiments, the hydroxyl protecting group is acetyl.
Some embodiments are directed to the compound of Formula V-3, a geometric isomer thereof, or a salt thereof. R⁵⁰, R⁵¹, and R⁵²in Formula V-3 are described hereinabove. In some embodiments, R⁵³is H. In some embodiments, R⁵³is an optionally substituted alkyl (e.g., optionally substituted C_1-6alkyl). In some embodiments, R⁵³is methyl. In some embodiments, R⁵³is ethyl.
In some embodiments, the compound of Formula V-1, Formula V-2, Formula V-3, or Formula V-4 is isolated. In some embodiments, the isolated compound of Formula V-1, Formula V-2, Formula V-3, or Formula V-4 is substantially pure (e.g., at least 80%, at least 85%, at least 90%, at least 95%, or at least 98% pure by weight). In some embodiments, the isolated compound of Formula V-1, Formula V-2, Formula V-3, or Formula V-4 is about 80%, about 85%, about 90%, about 95%, about 98% pure by weight, or any ranges between the specified values. In some embodiments, the isolated compound of Formula V-1, Formula V-2, Formula V-3, or Formula V-4 is substantially free (e.g., less than 5%, less than 3%, less than 2%, less than 1%, less than 0.5%, or less than 0.1% by weight) of other diastereomers and/or geometric isomer. In some embodiments, the isolated compound of Formula V-1, Formula V-2, Formula V-3, or Formula V-4 has about 5%, about 3%, about 2%, about 1%, about 0.5%, about 0.1% by weight, or any ranges between the specified values, of other diastereomers and/or geometric isomer. In some embodiments, the isolated compound of Formula V-1, Formula V-2, Formula V-3, or Formula V-4 is free of (i.e., non-detectable using current analytical tools) other diastereomers and/or geometric isomer.
In some embodiments, the synthetic intermediate is a compound of Formula V-5, Formula V-6, or Formula V-7, or a compound of Formula V-8 or a geometric isomer or a salt thereof:
wherein each of R⁵⁴and R⁵⁵is independently H or a hydroxyl protecting group, and R⁵⁶is H or an optionally substituted alkyl, provided that when R⁵⁴and R⁵⁵are both acetyl, then R⁵⁶is not methyl.
R⁵⁴and R⁵⁵in each of Formula V-5, Formula V-6, Formula V-7, and Formula V-8 can be independently selected. In some embodiments, R⁵⁴and R⁵⁵are each H. In some embodiments, R⁵⁴and R⁵⁵are each independently a hydroxyl protecting group (e.g., as described herein). In some embodiments, R⁵⁴and R⁵⁵are the same hydroxyl protecting group. In some embodiments, R⁵⁴and R⁵⁵are different. For example, one of R⁵⁴and R⁵⁵can be hydrogen and the other of R⁵⁴and R⁵⁵can be a hydroxyl protecting group. Suitable hydroxyl protecting groups include any of those described herein. In some embodiments, the hydroxyl protecting group is acetyl.
Some embodiments are directed to the compound of Formula V-8, a geometric isomer thereof, or a salt thereof. R⁵⁴, R⁵⁵, and R⁵⁶in Formula V-8 are described hereinabove. In some embodiments, R⁵⁶is H. In some embodiments, R⁵⁶is an optionally substituted alkyl (e.g., optionally substituted C_1-6alkyl), provided that when R⁵⁴and R⁵⁵are both acetyl, then R⁵⁶is not methyl. In some embodiments, R⁵⁶is ethyl.
In some embodiments, the compound of Formula V-5, Formula V-6, Formula V-7, or Formula V-8 is isolated. In some embodiments, the isolated compound of Formula V-5, Formula V-6, Formula V-7, or Formula V-8 is substantially pure (e.g., at least 80%, at least 85%, at least 90%, at least 95%, or at least 98% pure by weight). In some embodiments, the isolated compound of Formula V-5, Formula V-6, Formula V-7, or Formula V-8 is about 80%, about 85%, about 90%, about 95%, about 98% pure by weight, or any ranges between the specified values. In some embodiments, the isolated compound of Formula V-5, Formula V-6, Formula V-7, or Formula V-8 is substantially free (e.g., less than 5%, less than 3%, less than 2%, less than 1%, less than 0.5%, or less than 0.1% by weight) of other diastereomers and/or geometric isomer. In some embodiments, the isolated compound of Formula V-5, Formula V-6, Formula V-7, or Formula V-8 has about 5%, about 3%, about 2%, about 1%, about 0.5%, about 0.1% by weight, or any ranges between the specified values, of other diastereomers and/or geometric isomer. In some embodiments, the isolated compound of Formula V-5, Formula V-6, Formula V-7, or Formula V-8 is free of (i.e., non-detectable using current analytical tools) other diastereomers and/or geometric isomer.
In some embodiments, the synthetic intermediate is a compound having a structure of
wherein R³¹, R³²and R³³are each independently H or a hydroxyl protecting group. In some embodiments, R³¹, R³², and R³³are each H. In some embodiments, R³¹, R³², and R³³are each independently a hydroxyl protecting group (e.g., as described herein). In some embodiments, R³¹, R³², and R³³are the same hydroxyl protecting group. In some embodiments, at least two of R³¹, R³², and R³³are the same hydroxyl protecting group. In some embodiments, at least two of R³¹, R³², and R³³are different. For example, one of R³¹, R³², and R³³can be hydrogen and the other two of R³¹, R³², and R³³can be the same or different hydroxyl protecting group. Suitable hydroxyl protecting groups for Formula III-8 include any of those described herein. In some embodiments, the hydroxyl protecting group is acetyl.
In some embodiments, the compound of Formula III-8 is isolated. In some embodiments, the isolated compound of Formula III-8 is substantially pure (e.g., at least 80%, at least 85%, at least 90%, at least 95%, or at least 98% pure by weight). In some embodiments, the isolated compound of Formula III-8 is about 80%, about 85%, about 90%, about 95%, about 98% pure by weight, or any ranges between the specified values. In some embodiments, the isolated compound of Formula III-8 is substantially free (e.g., less than 5%, less than 3%, less than 2%, less than 1%, less than 0.5%, or less than 0.1% by weight) of other diastereomers. In some embodiments, the isolated compound of Formula III-8 has about 5%, about 3%, about 2%, about 1%, about 0.5%, about 0.1% by weight, or any ranges between the specified values, of other diastereomers. In some embodiments, the isolated compound of Formula III-8 is free of (i.e., non-detectable using current analytical tools) other diastereomers.
Pharmaceutical or Cosmetic Compositions
Certain embodiments of the present disclosure are directed to pharmaceutical or cosmetic compositions comprising one or more of CA, DCA, CDCA and their esters and salts, which are free of animal derived impurities.
In some embodiments, the pharmaceutical or cosmetic composition comprises an effective amount of high purity (e.g., a purity of at least 98%, at least 99%, at least 99.5%, or at least 99.9% by weight) cholic acid or a pharmaceutically or cosmetically acceptable salt thereof, or a high purity cholic acid composition herein, and a pharmaceutically or cosmetically acceptable excipient. In some embodiments, the cholic acid or pharmaceutically or cosmetically acceptable salt thereof has at least a purity of 99% by weight, for example, 99.2%, 99.5%, 99.9%, or greater than 99.9% by weight. In any of the embodiments described herein, the cholic acid or pharmaceutically or cosmetically acceptable salt thereof can also be characterized by being free of any animal derived impurities. In some embodiments, the pharmaceutical or cosmetic composition further comprises an effective amount of high purity (e.g., a purity of at least 98%, at least 99%, at least 99.5%, or at least 99.9% by weight) chenodeoxycholic acid or a pharmaceutically or cosmetically acceptable salt thereof. In any of the embodiments described herein, the chenodeoxycholic acid or pharmaceutically or cosmetically acceptable salt thereof can also be characterized by being free of any animal derived impurities. In some embodiments, the pharmaceutical or cosmetic composition further comprises an effective amount of high purity (e.g., a purity of at least 98%, at least 99%, at least 99.5%, or at least 99.9% by weight) deoxycholic acid or a pharmaceutically or cosmetically acceptable salt thereof. In any of the embodiments described herein, the deoxycholic acid or pharmaceutically or cosmetically acceptable salt thereof can also be characterized by being free of any animal derived impurities.
The high purity cholic acid can be obtained, for example, by the synthetic methods provided herein. In some embodiments, the present disclosure also provides a high purity cholic acid composition. Typically, the high purity cholic acid composition herein comprises cholic acid or a salt thereof, along with one or more process impurities. For example, in some embodiments, the high purity cholic acid composition comprises, consists essentially of, or consists of, cholic acid or a salt thereof, and one or more (e.g., 1, 2, 3, or all) compounds selected from Compounds 32-35 (see Example 6) or a salt thereof. In some embodiments, the high purity cholic acid composition can comprise any one of Compounds 32-35, or a salt thereof, any two of Compounds 32-35, or a salt thereof, any three of Compounds 32-35, or a salt thereof, or all of Compounds 32-35, or a salt thereof. In some embodiments, the total amount of Compounds 32-35 and salts thereof, as applicable, is less than 5% (e.g., less than 5%, less than 4%, less than 3%, less than 2%, less than 1%, less than 0.5%, less than 0.2%, less than 0.1%), more preferably, less than 2%, or less than 1% (e.g., less than 0.5%, less than 0.2%, less than 0.1%), by weight and/or by HPLC area of the cholic acid and salt thereof. For the avoidance of doubt, when the purity level is recited as weight percentage, the respective compound and all of its salt forms are considered, and the equivalent weight of the free acid form is then used for the weight percentage calculation. For example, when it is said that the weight percentage of Compound 32 and salt is less than 1% of the cholic acid and salt thereof, it should be understood that the equivalent weight of Compound 32 as free acid, after considering all salt forms of Compound 32 that may be present in the composition, is less than 1% by weight of cholic acid, also expressed as equivalent weight of the free acid, after considering all salt forms of cholic acid that may be present in the composition. Methods for determining purity of a composition are known to those skilled in the art. For example, the purity of the cholic acid composition herein can be analyzed based on weight, HPLC area, or both. Those skilled in the art would know how to validate an analytical method in determining such purity level. In any of the embodiments described herein, the purity of the cholic acid composition herein can be based on weight percentages.
Each of Compounds 32-35 or a salt thereof, if present in the high purity cholic acid composition, should be preferably less than 5% (e.g., less than 5%, less than 4%, less than 3%, less than 2%, less than 1%, less than 0.5%, less than 0.2%, less than 0.1%), more preferably, less than 2%, or less than 1% (e.g., less than 0.5%, less than 0.2%, less than 0.1%), by weight and/or by HPLC area of the cholic acid and salt thereof. For example, in some embodiments, the high purity cholic acid composition comprises Compound 32, or a salt thereof, in an amount of less than 1% (e.g., less than 0.5%, less than 0.2%, less than 0.1%) by weight and/or by HPLC area of the cholic acid and salt thereof. In some embodiments, the high purity cholic acid composition comprises Compound 33, or a salt thereof, in an amount of less than 5% (e.g., less than 5%, less than 4%, less than 3%, less than 2%, less than 1%, less than 0.5%, less than 0.2%, less than 0.1%), more preferably, less than 2%, or less than 1% (e.g., less than 0.5%, less than 0.2%, less than 0.1%), by weight and/or by HPLC area of the cholic acid and salt thereof. In some embodiments, the high purity cholic acid composition comprises Compound 34, or a salt thereof, in an amount of less than 1% (e.g., less than 0.5%, less than 0.2%, less than 0.1%) by weight and/or by HPLC area of the cholic acid and salt thereof. In some embodiments, the high purity cholic acid composition comprises Compound 35, or a salt thereof, in an amount of less than 5% (e.g., less than 5%, less than 4%, less than 3%, less than 2%, less than 1%, less than 0.5%, less than 0.2%, less than 0.1%), more preferably, less than 2%, or less than 1% (e.g., less than 0.5%, less than 0.2%, less than 0.1%), by weight and/or by HPLC area of the cholic acid and salt thereof. In some embodiments, the high purity cholic acid composition comprises cholic acid or salt thereof with a purity of at least 95% (e.g., at least 96%, at least 97%, at least 98%, or at least 99%). In some embodiments, the high purity cholic acid composition comprises cholic acid or salt thereof with a purity of at least 99% by weight and/or HPLC area, for example, 99.2%, 99.5%, 99.9%, or greater than 99.9% by weight, by HPLC area, or both.
The levels of Compounds 32-35 in a high purity cholic acid composition herein can be controlled. For example, when needed, further purification of a cholic acid composition herein can be made to reduce the level of (or even eliminate) one or more of Compounds 32-35. For example, as shown in FIG. 2, these impurities can be readily separated by HPLC, which has baseline separation in FIG. 2. See also Example 6, where each of Compounds 32-35 were isolated by reverse phase HPLC and characterized and identified. Although typically not desired, the level of one or more of Compounds 32-35 can also be increased by adding the respective substantially pure (e.g., at least 80%, at least 85%, at least 90%, at least 95%, or at least 98% pure by weight) Compounds 32-35 to the high purity cholic acid composition. Compounds 32-35 are themselves also useful, for example, they can be useful in quality control for cholic acid manufacturing, in identifying sources of cholic acid, or identifying potential synthetic pathways to such cholic acid.
The pharmaceutical or cosmetic composition comprising cholic acid or a pharmaceutically or cosmetically acceptable salt thereof can be formulated in various forms with suitable excipient(s). Suitable excipients can be any solid, liquid, semi-solid or, in the case of an aerosol composition, gaseous excipient that is generally available to one of skill in the art. Solid excipients include starch, cellulose, talc, glucose, lactose, sucrose, gelatin, malt, rice, flour, chalk, silica gel, magnesium stearate, sodium stearate, glycerol monostearate, sodium chloride, dried skim milk and the like. Examples of liquid and semisolid excipients can include glycerol, propylene glycol, water, ethanol and various oils, including those of petroleum, vegetable or synthetic origin, e.g., peanut oil, soybean oil, mineral oil, sesame oil, etc. Examples of liquid carriers, particularly for injectable solutions, include water, saline, aqueous dextrose, and glycols.
In some embodiments, the pharmaceutical or cosmetic composition is preferably an aqueous solution, e.g., suitable for subcutaneous injection. In some embodiments, the aqueous solution comprises: (i) high purity cholic acid, chenodeoxycholic acid, a pharmaceutically or cosmetically acceptable salt thereof, or a combination thereof; e.g., a high purity cholic acid composition described herein; and (ii) a pharmaceutical, veterinary, or cosmetic excipient. In some embodiments, the solution can further comprise a second therapeutic agent selected from the group consisting of: anti-microbial agents, vasoconstrictors, anti-thrombotic agents, anti-coagulation agents, suds-depressants, anti-inflammatory agents, analgesics, dispersion agents, anti-dispersion agents, penetration enhancers, steroids, tranquilizers, muscle relaxants, and anti-diarrhea agents. In some embodiments, a solution is in a container that contains up to 500 mL of solution. Such container can be a syringe or syringe-loadable container.
Non-limiting exemplary methods for formulating the cholic acid or pharmaceutically or cosmetically acceptable salt thereof include those described in WO 2015/198150, titled “Methods and Compositions of Bile Acids and Salts for Reduction of Fat,” the content of which is hereby incorporated by reference in its entirety.
Method of Use
Cholic acid, deoxycholic acid, and chenodeoxycholic acid are known to have medical and/or cosmetic uses. For example, WO 2015/198150 describes the use of cholate and/or chenodeoxycholate to reduce subcutaneous fat accumulations in a mammal by local administration to a target site. WO 2015/198150 also describes that cholate and/or chenodeoxycholate compositions can be used for dissolving lipomas, fat, mesotherapy, separating tissue, tumor reduction, cancer reduction, cancer treatment, and any other clinical situation where one might want to use loosen, remove, assist the body consumption or resolution of wax, lipids, proteins, or carbohydrates from a part or region of the body. For example, in the treatment of lipomas, the cholate and/or chenodeoxycholate compositions can be injected subcutaneously in contact with the lipomas to lyse the lipomas. Methods of using deoxycholic acid are described, for example, in U.S. Pat. No. 7,622,130, and U.S. Application Publication Nos. 2005-0267080 A1 and 2006-0127468 A1, etc.
The CA, DCA, CDCA, and their esters and salts described herein can also be used for any of the known medical and/or cosmetic uses. In some embodiments, the present disclosure also provides a method for non-surgical removal of a localized fat deposit in a subject. In some embodiments, the method comprises administering to the subject an effective amount of any of the pharmaceutical or cosmetic compositions described hereinabove, for example, a pharmaceutical or cosmetic composition comprising a fat-lysing effective amount of high purity (e.g., a purity of at least 98%, at least 99%, at least 99.5%, or at least 99.9% by weight) cholic acid, chenodeoxycholic acid, a pharmaceutically or cosmetically acceptable salt thereof, or a combination thereof, e.g., a high purity cholic acid composition described herein, and a pharmaceutically or cosmetically acceptable excipient.

EXAMPLES

The various starting materials, intermediates, and compounds of the preferred embodiments can be isolated and purified where appropriate using conventional techniques such as precipitation, filtration, crystallization, evaporation, distillation, and chromatography. Characterization of these compounds can be performed using conventional methods such as by melting point, mass spectrum, nuclear magnetic resonance, and various other spectroscopic analyses. Exemplary embodiments of steps for performing the synthesis of products described herein are described in greater detail infra. Table 1 describes abbreviations used to express various compounds/moieties/apparatus/procedure/property in the exemplary reaction schemes and synthetic routes described in the following examples and throughout the specification.

TABLE 1

List of Abbreviations

Ac	Acetyl
Ac-DHEA	Acetyl-dehydroepiandrosterone
CA	Cholic acid
CDCA	Chenodeoxycholic acid
Cu(OTf)₂	Copper triflate
DCA	Deoxycholic acid
DCM	Dichloromethane
DDQ	Dichloro-5,6-dicyano-1,4-benzoquinone
DHEA	Dehydroepiandrosterone
DMAP	4-dimethylaminopyridine
DMF	N,N-dimethylformamide
DMSO	Dimethyl sulfoxide
EDTA	Ethylenediaminetetraacetic acid
Et	ethyl
Et₃N	triethylamine
EtOAc/EA	ethyl acetate
EtOH	ethanol
Eq/equiv	equivalent
g(s)	gram(s)
h/hr(s)	hour(s)
HPLC	High pressure liquid chromatography
¹H NMR	Proton nuclear magnetic resonance
mCPBA/MCPBA	3-Chloroperoxybenzoic acid
Me	methyl
MeOH	methanol
mL	milliliter(s)
mg	milligram(s)
mol	mole(s)
mmol	millimole(s)
min(s)	minute(s)
NaH	Sodium hydride
NCS	N-chlorosuccinimide
NBS	N-bromosuccinimide
PE	petroleum ether
PTSA/p-TsOH	Para-toluenesulfonic acid
Py	pyridine
pre-TLC/prep-TLC	Preparative thin layer chromatography
rt	Room tempreture
TEA	triethylamine
TEMPO	(2,2,6,6-tetramethylpiperidin-1-yl)oxidanyl
THF	tetrahydrofuran
TLC	Thin layer chromatography
μL/uL	microliter
μmol/umol	micromole(s)

Example 1

Synthesis of Cholic Acid from DHEA: Route 1

Step 1
To a flask was added DHEA (50.00 g, 173.36 mmol, 1.00 eq) and DCM (1.00 L) at 15° C. Then, it was cooled to 0° C., TEMPO (2.73 g, 17.34 mmol, 0.10 eq) was added, and DDQ (86.58 g, 381.39 mmol, and 2.20 eq) was added in portions at 0° C. The reaction mixture was warmed to 25° C. and stirred at 25° C. for 48 hrs, TLC showed one new main spot was formed. The reaction was filtered, and the filtrate was washed with saturated Na₂SO₃(100 mL*2) and brine (200 mL*2). The combined organic phase was dried with anhydrous Na₂SO₄and concentrated in vacuum. The residue was purified by column chromatography (PE/EA=50/1 to 10/1) to afford Compound 1 (39.0 g, 135.35 mmol, 78.08% yield) as white solid. ¹H NMR, (400 MHz, CDCl3); δ=6.21 (s, 2H), 5.78-5.71 (m, 1H), 2.66-2.55 (m, 1H), 2.53-2.46 (m, 1H), 2.46-2.35 (m, 2H), 2.22-2.13 (m, 2H), 2.08-2.01 (m, 1H), 1.92 (td, J=3.1, 13.0 Hz, 1H), 1.80-1.69 (m, 3H), 1.56-1.50 (m, 1H), 1.48-1.42 (m, 1H), 1.39-1.32 (m, 1H), 1.32-1.24 (m, 1H), 1.16 (s, 3H), 0.99 (s, 3H).
Step 2
To a solution of compound 1(39.00 g, 137.14 mmol, 1.00 eq) in DCM (400.00 mL) was drop-wise added m-CPBA (61.25 g, 301.71 mmol, 87% purity, 2.20 eq) which dissolved in DCM (50.00 mL) to the reaction at 5° C. The resulting solution was stirred at 5° C. for 24h, TLC showed the starting material was consumed completely, and one new main spot was formed. Then the reaction was filtered and the filtrate was washed with saturated Na₂SO₃solution (200 mL) at 25° C. for 0.5h. The organic phase was separated and concentrated in vacuum. The residue was purified by column chromatography (PE/EA=20/1 to 5/1) to afford Compound 2 (16.12 g, 51.79 mmol, 37.76% yield) as white solid. ¹H NMR, (400 MHz, CDCl₃): δ=6.16 (s, 1H), 3.55 (d, J=3.8 Hz, 1H), 3.48 (d, J=3.6 Hz, 1H), 2.63-2.50 (m, 3H), 2.30-2.22 (m, 2H), 2.21-2.14 (m, 1H), 1.98 (ddd, J=2.3, 5.3, 13.2 Hz, 2H), 1.91-1.85 (m, 2H), 1.80-1.72 (m, 4H), 1.43-1.33 (m, 3H), 1.30-1.25 (m, 2H), 1.15 (s, 3H), 0.97 (s, 3H).
Step 3
To a solution of compound 2 (16.12 g, 53.66 mmol, 1.00 eq) in Py (160.00 mL) was added Pd/C (10%, 1.70 g) under N₂protection at 25° C. The suspension was degassed under vacuum and purged with H₂several times. The reaction was stirred (50 psi) at 25° C. for 12 hours under H₂atmosphere, TLC showed the starting material was consumed completely and one new main spot was formed. The reaction mixture was filtered with celite, the filtrate was washed with 5% HCl (100 mL) and extracted with EA (150 mL). The organic phase was separated and concentrated in vacuum to give 16.34 g of crude compound 3 as light yellow solid.
Step 4
To a solution of compound 3 (16.34 g, 53.68 mmol, 1.00 eq) in THF (150.00 mL) was drop-wise added LiAlH(t-BuO)₃(16.99 g, 67.10 mmol, 18.88 mL, 1.25 eq) which was dissolved in THF (30.00 mL) at 5° C., The resulting solution was stirred at 5° C. for 0.5 hr. TLC showed one main spot was formed. The reaction mixture was quenched by 5% HCl (50 ml), extracted with EA (50 ml*2), the organic phase was separated and concentrated in vacuum. The residue was purified by silica gel chromatography (PE/EA=5:1 to 2:1) to afford Compound 4 (6.20 g, 35.62% yield) as colorless oil.
Step 5
To a mixture of compound 4 (6.00 g, 19.58 mmol, 1.00 eq) and 2-pyridylmethanamine (10.59 g, 97.90 mmol, 9.99 mL, 5.00 eq) in toluene (50.00 mL) was added PTSA (101.15 mg, 0.58 mmol, 0.03 eq) at 25° C., the mixture was stirred at 120° C. for 2 hrs with Dean-Stark apparatus. Then the reaction mixture was diluted with EA (100 ml), washed with saturated NaHCO₃(20 ml) and water (50 ml). The organic phase was separated and concentrated in vacuum. The residue was triturated by EA (10 ml) at 25° C. to afford Compound 5 (4.12 g, 10.13 mmol, 51.73% yield, and 96.5% purity) as white solid. ¹H NMR, (400 MHz, CDCl₃): δ=8.45 (br d, J=4.5 Hz, 1H), 7.63-7.56 (m, 1H), 7.34 (d, J=7.8 Hz, 1H), 7.10-7.04 (m, 1H), 4.61-4.47 (m, 2H), 3.91 (br s, 1H), 3.47-3.36 (m, 2H), 2.46-2.36 (m, 1H), 2.31-2.20 (m, 1H), 2.18-2.07 (m, 2H), 2.01-1.92 (m, 2H), 1.91-1.78 (m, 3H), 1.68-1.62 (m, 2H), 1.48-1.39 (m, 2H), 1.38-1.25 (m, 5H), 1.20-1.11 (m, 2H), 0.98-0.93 (m, 1H), 0.88 (s, 3H), 0.83 (s, 3H).
Step 6
To a solution of compound 5 (2.00 g, 5.04 mmol, 1.00 eq) in acetone (10.00 mL) and MeOH (10.00 mL) was added sodium ascorbate (2.00 g, 10.09 mmol, 2.0 eq) and Cu(MeCN)₄PF₆(2.63 g, 7.06 mmol, 1.40 eq) in sequence at 25° C. After stirred at 25° C. for 5 mins, oxygen (15 psi) was bubbled through the reaction mixture for 10 mins. Then the reaction was heated to 50° C. and stirred at 50° C. for 2 hrs under O₂atmosphere. TLC showed one new main spot was formed. After cooled to 25° C., EA (100 mL) and saturated EDTA-Na₄(50 mL) was added, the mixture was stirred at 25° C. for 30 mins. The organic phase was separated and concentrated in vacuo. The residue was purified by column chromatography (PE/EA=3/1 to 1/2) to afford Compound 6 (0.25 g, 0.75 mmol, 14.95% yield) as light yellow solid.
Step 7
EtPPh3Br (2.41 g, 6.50 mmol, 5.00 eq) in a flask was dried under high vacuum for 1 hr at 70° C. Dry THF (3.00 mL) was added, followed by adding t-BuOK (0.73 g, 6.50 mmol, 5.00 eq) under N₂. The entire mixture was still stirred at 70° C. for 0.5 hr under N₂. The solution of Compound 6 (0.42 g, 1.30 mmol, 1.00 eq) in dry THF (2.00 mL) was added to the reaction at 70° C., the reaction was stirred at 70° C. for another 2 hrs. TLC showed the starting material was all consumed and one new main spot was formed. After cooling to room temperature, the solution was diluted by EA (40 mL), quenched by saturated NaCl solution (10 mL). The aqueous solution was extracted with EA (20 mL), dried over Na₂SO₄, concentrated in vacuum to give 0.48 g as light yellow oil.
The obtained yellow oil (0.48 g, 1.45 mmol, 1.00 eq) was dissolved in DCM (5.00 mL) at 25° C., followed by adding Ac₂O (621.97 mg, 6.05 mmol, 570.62 uL, 4.2 eq), Et₃N (0.88 g, 8.70 mmol, 1.21 mL, 6.00 eq) and DMAP (35.44 mg, 290.11 umol, 0.20 eq) at 25° C. The reaction was stirred at 25° C. for 7 hrs. TLC showed the starting material was consumed completely and one new main spot was formed. The reaction was diluted with EA (50 ml), washed with H₂O (10 mL), dried over Na₂SO₄and filtered, the organic layer was concentrated in vacuo. The residue was purified by column chromatography (PE/EA=50/1 to 20/1) to give Compound 7(0.18 g, 0.38 mmol, 26.28% yield) as colorless oil.
Step 8
To a 50 ml flask was added compound 7 (50.00 mg, 108.55 umol, 1.00 eq) and DCM (5.00 mL). The reaction was cooled to 0° C.; paraformaldehyde (48.89 mg, 542.77 umol, 5.00 eq) was added to the reaction at 0° C., followed by adding BF₃.Et₂O (1.54 mg, 10.86 umol, 1.34 uL, 0.10 eq) to the reaction at 0° C. The reaction was warmed to 15° C., and stirred at 15° C. for 2 hrs. TLC showed one new main spot was formed. The reaction was diluted with DCM (50 ml), and quenched by water (30 ml). The organic layer was concentrated in vacuo. The residue was purified by pre-TLC (PE/EA=1/1) to give colorless oil residue (40 mg).
To a 10 ml tube was added (COCl)₂(61.84 mg, 487.18 umol, 42.65 uL, 2.40 eq) and DCM (6.00 mL). The mixture was cooled to −78° C. DMSO (63.44 mg, 811.96 umol, 63.44 uL, 4.00 eq) was added drop-wise to the reaction mixture at −78° C. After addition, the reaction was stirred at −78° C. for 0.5 hrs. The colorless oil residue which previous prepared (two batches, 100 mg) was added to the reaction at −78° C. It was stirred at −78 for 0.5 hrs. TEA (205.41 mg, 2.03 mmol, 281.38 uL, 10.00 eq) was added drop-wise at −78° C. After addition, the reaction was warmed to 15° C., and it was stirred at 15° C. for 0.5 hr. TLC showed one new main spot was formed. The reaction was diluted with DCM (50 ml), and quenched by water (30 ml). The separated organic layer was concentrated in vacuo. The residue was purified by pre-TLC to give compound 8 (50 mg, 50% yield).
Step 9
To a 10 ml tube was added NaH (32.75 mg, 818.64 umol, 60% purity, 4.00 eq) and THF (2.00 mL), followed by adding ethyl 2-diethoxyphosphorylacetate (229.41 mg, 1.02 mmol, 203.02 uL, 5.00 eq) to the flask at 15° C. The resulting mixture was stirred at 15° C. for 10 mins. Compound 8 (100.00 mg, 204.66 umol, 1.00 eq) was added to the flask at 15° C., and it was stirred at 15° C. for 0.5 hr, TLC showed one new main spot was formed. The reaction was quenched by water (5 ml), and extracted with DCM (2*30 ml). The organic layer was concentrated in vacuo. The residue was purified by pre-TLC to give compound 9 (100 mg, 87.46% yield) as colorless oil. Mass (m/z): 581.4 (M⁺+23).
Step 10
To a 35 ml pressure tube was added compound 9 (100.00 mg, 178.99 umol, 1.00 eq), EtOH (4.00 mL) and Pd/C (10.00 mg) at 25° C. The mixture was purged with H₂gas (50 psi) and stirred at 25° C. for 4 hrs. TLC showed one new main spot was formed. The resulting mixture was filtered, the filtrate was concentrated in vacuo to give compound 10 (60 mg, crude) as colorless oil. Mass (m/z): 585.4 (M⁺+23).
Step 11
To a solution of compound 10 (60.00 mg, 106.62 umol, 1.00 eq) in MeOH (4.00 mL) was added NaOH (51.18 mg, 1.28 mmol, 12.00 eq) at 25° C. After that, the reaction was heated to 60° C., and it was stirred at 60° C. for 2 hrs. TLC showed one new main spot was formed. The reaction was cooled to rt, concentrated in vacuo to remove solvent, the resulting residue was dissolved in water (5 ml), acidized by HCl to PH=3. The resulting aqueous layer was extracted with EA (3*15 ml). The organic layer was concentrated in vacuo to give compound 10 (70 mg, crude) as white solid. Mass (m/z): 431.1 (M⁺+23), 839.1 (2M⁺+23).
Step 12
To a solution of compound 11 (70 mg, 160.32 umol, 1.00 eq) in acetone (3 mL) was added Jones reagent (95.27 mg, 480.96 umol, 3 eq) drop-wise at 20° C. After addition, the reaction was stirred at 20° C. for 10 mins, TLC showed one new main spot was formed. The reaction was quenched by isopropanol (0.5 ml) and diluted with water (5 ml). The resulting aqueous mixture was extracted with EA (3*15 ml). The organic layer was concentrated in vacuo. The residue was purified by pre-TLC to give compound 12 (20 mg, 48 umol, 29.9% yield) as white solid. Mass (m/z): 403.3 (M⁺+1).
Step 13
To a solution of compound 12 (10.00 mg, 24.84 umol, 1 eq) in THF (5 mL) was added LiAlH(t-BuO)₃(94.38 mg, 372.65 umol, 104.40 uL, 15 eq) at 20° C. It was stirred at 20° C. for 1 hr and at 40° C. for 16 hrs, TLC showed one new main spot was formed. The reaction was diluted by EA (50 ml), quenched by HCl (10 ml, 1M). The separated organic layer was concentrated in vacuo. The residue was purified by pre-TLC to give CA (3 mg, 29.6% yield) as white solid. ¹H NMR, (400 MHz, DMSO-d6): δ=11.91 (br s, 1H), 4.30 (d, J=4.39, 1H), 4.10 (d, J=3.51, 1H), 3.99 (d, J=3.26, 1H), 3.79 (br d, J=2.64, 1H), 3.62 (br s, 1H), 3.23-3.14 (m, 1H), 2.27-2.06 (m, 5H), 1.82-1.63 (m, 6H), 1.41-1.13 (m, 11H), 1.01-0.96 (m, 1H), 0.92 (d, J=6.4, 3H), 0.88-0.84 (m, 1H), 0.81 (s, 3H), 0.59 (s, 3H). ¹³C NMR, (100 MHz, DMSO-d6): δ=175.45, 71.45, 70.90, 66.71, 46.56, 46.22, 41.99, 41.84, 35.78, 35.52, 35.34, 34.86, 31.31, 31.27, 30.88, 29.01, 27.75, 26.68, 23.27, 23.10, 17.41, 12.81.

Example 2

Synthesis of Cholic Acid from DHEA: Route 2

Step 1
To a mixture of DHEA (20.00 g, 69.34 mmol, 1.00 eq) and 2-pyridylmethanamine (37.49 g, 346.70 mmol, 35.37 mL, 5.00 eq) in toluene(250.00 mL) was added PTSA (358.23 mg, 2.08 mmol, 0.03 eq) at 25° C., the mixture was stirred at 120° C. for 2 hrs with Dean-Stark apparatus. Then the reaction mixture was diluted with EA (400 ml), washed with saturated NaHCO₃(100 ml*2) and water (100 ml*2). The organic layer was concentrated. The residue was triturated by EA (30 ml) at 25° C. to afford Compound 13 (23.40 g, 57.80 mmol, 83.36% yield, 93.5% purity) as white solid. ¹H NMR, (400 MHz, CDCl₃): δ=8.54 (d, J=4.1 Hz, 1H), 7.67 (dt, J=1.7, 7.7 Hz, 1H), 7.45 (d, J=7.8 Hz, 1H), 7.19-7.11 (m, 1H), 5.40 (br d, J=4.9 Hz, 1H), 4.70-4.53 (m, 2H), 3.63-3.48 (m, 1H), 2.54-2.43 (m, 1H), 2.38-2.25 (m, 3H), 2.15-2.03 (m, 2H), 1.96-1.85 (m, 3H), 1.75-1.70 (m, 1H), 1.64-1.36 (m, 6H), 1.26-1.10 (m, 2H), 1.07 (s, 3H), 1.03 (br d, J=4.5 Hz, 1H), 0.94 (s, 3H).
Step 2
To a solution of compound 13 (6.00 g, 15.85 mmol, 1.00 eq) in acetone (60.00 mL) and MeOH (60.00 mL) was added sodium ascorbate (6.28 g, 31.70 mmol, 2.00 eq) and Cu(MeCN)₄PF₆(8.27 g, 22.19 mmol, 1.40 eq) in sequence at 25° C. After stirred at 25° C. for 5 mins, oxygen (15 psi) was bubbled through the reaction mixture for 10 mins. Then the reaction was heated to 50° C. and stirred at 50° C. for 2 hrs under O₂atmosphere. After cooled to 25° C., EA (300 mL) and saturated EDTA-Na₄(100 mL) was added, the mixture was stirred at 25° C. for 30 mins. The organic phase was separated and concentrated in vacuo. The residue was purified by column chromatography (PE/EA=15/1 to 5/1) to afford Compound 14 (2.40 g, 7.88 mmol, 49.74% yield) as white solid. ¹H NMR, (400 MHz, CDCl₃): δ=5.43-5.38 (m, 1H), 3.88-3.79 (m, 1H), 3.59-3.51 (m, 1H), 3.09 (s, 1H), 2.51 (dd, J=8.4, 19.4 Hz, 1H), 2.40-2.33 (m, 1H), 2.31-2.22 (m, 1H), 2.20-2.09 (m, 2H), 2.08-1.97 (m, 1H), 1.93-1.86 (m, 2H), 1.76-1.63 (m, 3H), 1.56-1.45 (m, 3H), 1.33-1.24 (m, 1H), 1.20-1.09 (m, 2H), 1.07 (s, 3H), 0.99 (s, 3H).
Step 3
EtPPh₃Br (12.18 g, 32.80 mmol, 10.00 eq) in a flask was dried under high vacuum for 1 hr at 70° C. Dry THF (100.00 mL) was added, followed by adding t-BuOK (3.68 g, 32.80 mmol, 10.00 eq) under N2. The entire mixture was still stirred at 70° C. for 0.5 hr under N₂. The solution of Compound 14 (1.00 g, 3.28 mmol, 1.00 eq) in dry THF (5.00 mL) was added to the reaction at 70° C., the reaction was stirred at 70° C. for another 2 hrs. TLC showed the starting material was all consumed and one new main spot was formed. After cooling to room temperature, the solution was diluted by EA (100 mL), quenched by saturated NH₄Cl solution (30 mL). The aqueous solution was extracted with EA (50 mL*2), the combined organic layers were washed with brine (40 mL), dried over Na₂SO₄and filtered, concentrated in vacuum to give 1.04 g light yellow oil.
The obtained light yellow oil (1.04 g, 3.29 mmol, 1.00 eq) was dissolved in DCM (20.00 mL) at 25° C., followed by adding Ac2O (752.36 mg, 7.37 mmol, 690.24 uL, 2.24 eq), Et₃N (2.00 g, 19.74 mmol, 2.74 mL, 6.00 eq) and DMAP (80.39 mg, 658.00 umol, 0.20 eq) at 25° C. The reaction was stirred at 25° C. for 3 hrs. TLC showed the starting material was consumed completely and one new main spot was formed. The reaction was diluted with EA (100 ml), washed with H₂O (50 mL), dried over Na₂SO₄and filtered, the organic layer was concentrated in vacuo. The residue was purified with column chromatography (PE/EA=30/1 to 20/1) to give Compound 15 (1.14 g, 2.62 mmol, 79.59% yield, 92% purity) as off-white oil. ¹H NMR, (400 MHz, CDCl₃): δ=5.41 (d, J=5.0 Hz, 1H), 5.31-5.19 (m, 1H), 4.91 (dd, J=5.0, 10.9 Hz, 1H), 4.68-4.57 (m, 1H), 2.46-2.19 (m, 4H), 2.12-2.08 (m, 3H), 2.07-2.04 (m, 3H), 1.98 (td, J=4.9, 13.0 Hz, 1H), 1.91-1.81 (m, 2H), 1.72-1.65 (m, 1H), 1.65-1.61 (m, 3H), 1.60-1.41 (m, 5H), 1.37-1.12 (m, 4H), 1.05 (s, 3H), 1.02 (s, 3H). Mass (m/z): 423.2 (M⁺+23).
Step 4
To a solution of compound 15 (1.12 g, 2.80 mmol, 1.00 eq) in DCM (100.00 mL) was added paraformaldehyde (2.7 g, 29.96 mmol, 10.7 eq) in one portion at 0° C., followed by adding BF₃.Et₂O (39.74 mg, 280.00 umol, 34.56 uL, 0.10 eq) at 0° C. The mixture was warmed to 25° C. and stirred at 25° C. for 2 hrs. TLC showed one new spot was formed. The reaction mixture was quenched by H₂O (15 ml), extracted with DCM (10 ml*3), dried with anhydrous Na₂SO₄, filtered and concentrated in vacuum. The residue was purified by silica gel chromatography (PE:EA=30:1 to 15:1) to afford 860 mg white solid.
To a flask was added oxalyl chloride (278.87 mg, 2.20 mmol, 192.32 uL, 1.10 eq) and DCM (20.00 mL) at 15° C. The reaction was cooled to −78° C. and it was stirred at −78° C. for 15 mins. Then, DMSO (312.10 mg, 3.99 mmol, 312.10 uL, 2.00 eq) was added drop-wise to the reaction at −78° C. The mixture was stirred at −78° C. for 15 mins. The solution of previous obtained white solid (860.00 mg, 2.00 mmol, 1.00 eq) in DCM (3.00 mL) was added into the reactor at −78° C. The mixture was stirred at −78° C. for another 15 mins. Then TEA (1.01 g, 9.99 mmol, 1.38 mL, 5.00 eq) was added drop-wise at −78° C. The resulting mixture was stirred at −78° C. for 15 mins and at 15° C. for 30 mins, TLC showed one new main spot was formed, the reaction was quenched with H₂O (5 mL), extracted with DCM (15 mL). The organic phase was washed with brine (50 mL*2), dried with anhydrous Na₂SO₄, filtered and concentrated in vacuum, which was purified by column chromatography (SiO2, PE:EA=60:1 to 30:1) to afford Compound 16 (510 mg, 59.5% yield) as a white solid.
Step 5
To a solution of NaH (476.00 mg, 11.90 mmol, 60% purity, 10.00 eq) in THF (10.00 ml) was added ethyl 2-diethoxyphosphorylacetate (2.67 g, 11.90 mmol, 2.36 mL, 10.00 eq) at 25° C., the reaction mixture was stirred at 25° C. for 30 mins. A solution of Compound 16 (510.00 mg, 1.19 mmol, 1.00 eq) in THF (2.00 mL) was added to the reaction at 25° C. and it was stirred at 25° C. for 2 hrs. TLC showed the starting material was all consumed. Saturated NH₄Cl (15 mL) was added into the reactor to quench the reaction. The mixture was extracted with EA (50 mL*3), the organic layer was washed with brine (100 mL*2), dried with anhydrous Na₂SO₄, filtered and concentrated in vacuum. The residue was purified by column chromatography (PE:EA=50/1 to 30:1) to afford compound 17 as colorless oil (720.00 mg, 929.00 umol, 78.07% yield, 64.34% purity by ¹H NMR). ¹H NMR, (400 MHz, CDCl₃): δ=7.01 (dd, J=6.9, 15.7 Hz, 1H), 5.80 (dd, J=1.3, 15.7 Hz, 1H), 5.45 (br s, 1H), 5.42 (br d, J=5.0 Hz, 1H), 4.93 (dd, J=5.1, 10.7 Hz, 1H), 4.67-4.58 (m, 1H), 4.21 (q, J=7.2, 14.7 Hz, 2H), 3.12 (q, J=6.7 Hz, 1H), 2.42-2.27 (m, 2H), 2.19-2.12 (m, 1H), 2.10 (s, 3H), 2.05 (s, 3H), 2.04-1.77 (m, 5H), 1.69-1.64 (m, 1H), 1.60-1.52 (m, 1H), 1.51-1.39 (m, 2H), 1.37 (t, J=7.1, 3H), 1.29-1.27 (m, 1H), 1.26-1.17 (m, 2H), 1.14 (d, J=6.8 Hz, 3H), 1.07 (s, 3H), 0.95 (s, 3H). Mass (m/z): 521.2 (M⁺+23).
Step 6
To a solution of compound 17 (660.00 mg, 1.32 mmol, 1.00 eq) in MeOH (150.00 mL) was added Pd/C (10%, 70 mg) at 15° C. The suspension was degassed under vacuum and purged with H₂several times. The reaction was heated to 25° C. and stirred at 25° C. for 12 hours under H₂(50 psi). LCMS showed the starting material was consumed completely. The reaction mixture was filtered and the filtrate was concentrated to give 450.00 mg white solid.
To a mixture of obtained white solid (350.00 mg, 693.48 umol, 1.00 eq) in EtOH (10.00 mL) was added NaOH (332.87 mg, 8.32 mmol, 12.00 eq) in one portion at 15° C. under N₂. The mixture was heated to 30° C. and stirred at 30° C. for 2 hrs. LCMS showed the starting material was all consumed, but no target mass was found. Another batch of NaOH (221.92 mg, 5.55 mmol, 8.00 eq) was added at 15° C., the mixture was keep stirred at 30° C. for another 4 hrs. LCMS showed all the starting material was consumed and desired mass was found. The mixture was concentrated in reduced pressure to remove solvent. Aqueous HCl (1N, 10 ml) was added to the residue to adjust to PH=4, and then extracted with EA (50 mL*3). The organic layer was washed with brine (10 mL*2), dried with anhydrous Na₂SO₄, filtered and concentrated in vacuum to afford white solid (260.00 mg, 662.30 umol, 95.50% yield).
The obtained white solid (260.00 mg) was dissolved in EtOH (15.00 mL) at 15° C., followed by adding HCl (153.00 mg, 4.20 mmol, 150.00 uL, 6.34 eq) at 15° C. The reaction mixture was heated to 80° C. and stirred at 80° C. for 30 mins. TLC showed the starting material was all consumed and two new spots were formed. The reaction was concentrated in vacuum, which was purified by column chromatography (SiO2, PE:EA=4:1 to 2:1) to give compound 18 (176 mg) as white solid. ¹H NMR, (400 MHz, CDCl₃): δ=4.14 (q, J=7.1 Hz, 2H), 3.66-3.54 (m, 1H), 3.41 (dd, J=4.7, 11.0 Hz, 1H), 2.44-2.33 (m, 1H), 2.31-2.18 (m, 1H), 1.99-1.89 (m, 1H), 1.88-1.79 (m, 2H), 1.76-1.67 (m, 4H), 1.64-1.59 (m, 2H), 1.54-1.42 (m, 4H), 1.33-1.30 (m, 3H), 1.30-1.27 (m, 5H), 1.26-1.16 (m, 3H), 1.15-1.09 (m, 1H), 1.03 (d, J=6.8 Hz, 3H), 0.98-0.86 (m, 2H), 0.83 (s, 3H), 0.74 (s, 3H). Mass (m/z): 443.3 (M⁺+23).
Step 7
Compound 18 (50 mg, 118.87 umol, 1.0 eq) was dissolved in acetone (2.50 mL) at 15° C., and then Jones reagent (150.00 uL) was added drop-wise to the solution until a yellow color persisted. The mixture was stirred at 15° C. for another 15 mins. TLC indicated desired product was formed. i-PrOH (10 ml) was added drop-wise and stirred 10 mins to quench the remaining Jones reagent. Then, H₂O (10 mL) was added the reaction mixture, and it was extracted with EA (50 mL*2). The combined organic phase was washed with brine (15 mL), dried with anhydrous Na₂SO₄, filtered and concentrated in vacuum. The residue was purified by column chromatography (PE:EA=50/1, 10/1) to afford compound 19 (30.00 mg, 72.01 umol, 60.58% yield) as a white solid. ¹H NMR, (400 MHz, DMSO): δ=4.05 (q, J=7.2 Hz, 2H), 2.55 (t, J=12.7 Hz, 1H), 2.36-2.23 (m, 3H), 2.22-2.10 (m, 3H), 2.10-2.03 (m, 1H), 2.00-1.85 (m, 2H), 1.85-1.63 (m, 5H), 1.50-1.42 (m, 1H), 1.38-1.25 (m, 7H), 1.21-1.16 (m, 4H), 1.15-1.09 (m, 1H), 1.03 (s, 3H), 0.99 (s, 3H), 0.92-0.84 (m, 1H), 0.79 (d, J=6.5 Hz, 3H). Mass (m/z): 417.3 (M⁺+1).
Step 8
To a 1000 ml flask was added 19 (20.00 g, 48.01 mmol, 1.00 eq) and AcOH (300.00 mL). The reaction was cooled to 0° C., and then fresh prepared Br₂(7.67 g, 48.01 mmol, 2.47 mL, 1.00 eq) in AcOH (60.00 mL) was added to the reaction drop-wise. After addition, the reaction was warmed to 20° C., and stirred at 20° C. for 0.5 hour. Then, the reaction was diluted with EA (200 ml), washed with water (200 ml) and saturated NaHCO₃(3*200 ml). The organic layer was concentrated in vacuo to give 20 g crude oil residue.
The oil residue (20.00 g) was added to a 1000 ml flask, DMF (500.00 mL) was added to the flask to prepare a solution, followed by adding Li₂CO₃(12.61 g, 170.72 mmol, 4.23 eq) and LiBr (6.20 g, 71.44 mmol, 1.79 mL, 1.77 eq) to the reaction mixture. Then the reaction was heated to 150° C., and it was stirred at 150° C. for 2 hrs. After TLC (PE/EA=2/1) showed the reaction was completed, the reaction was concentrated in vacuo, diluted with EA (500 ml), washed with water (500 ml), and the organic layer was concentrated in vacuo to give oil residue. The residue was purified by column chromatography to give compound 20 (5.4 g, 32% yield). ¹H NMR, (400 MHz, MeOD): δ=5.69 (s, 1H), 4.06 (q, J=7.1 Hz, 2H), 2.58 (t, 1H), 2.43-2.09 (m, 7H), 1.99-1.61 (m, 8H), 1.35-1.24 (m, 6H), 1.20 (s, 3H), 1.09 (t, J=8.0 Hz, 3H), 1.02 (s, 3H), 1.06-0.93 (m, 1H), 0.80 (d, J=6.4 Hz 3H). Mass (m/z): 415.1 (M⁺+1).
Step 9
To a 100 ml flask was added compound 20 (1.00 g, 2.41 mmol, 1.00 eq), AcOH (10.00 mL) and toluene (1.00 mL). 2,3,5,6-tetrachloro-1,4-benzoquinone (889.62 mg, 3.62 mmol, 1.50 eq) was added to the reaction mixture at rt. The reaction was heated to 120° C., and it was stirred at 120° C. for 2 hrs. After LCMS showed the reaction was completed, it was diluted with EA (100 ml), washed with water (100 ml) and saturated NaHCO₃(2*100 ml). The organic layer was concentrated in vacuo to give yellow oil residue. The residue was purified by column chromatography to give compound 21 (650 mg, 89% purity, 58% yield) as white solid. ¹H NMR, (400 MHz, CDCl₃): δ=6.20-6.12 (m, 2H), 5.74 (s, 1H), 5.41 (s, 1H), 4.16 (q, J=7.1, 2H), 2.73-1.32 (m, 19H), 1.28 (t, J=7.1, 3H), 1.22 (s, 3H), 1.15 (s, 3H), 0.90 (d, J=6.3, 3H). Mass (m/z): 413.1 (M⁺+1).
Step 10
To a 100 ml flask was added compound 21 (2.50 g, 6.06 mmol, 1.00 eq) and DCM (60.00 mL). The reaction was cooled to 0° C., m-CPBA (2.09 g, 8.48 mmol, 70% purity, 1.40 eq) was added to the flask at 0° C., and it was stirred at 0° C. for 48 hrs. After TLC (PE/EA=2/1) showed the reaction was completed, it was diluted with DCM (100 ml), washed with saturated Na₂SO₃(2*60 ml), the organic layer was concentrated in vacuo. The residue was purified by column chromatography (basified by TEA) to give compound 22 (1.0 g, 38% yield) as white solid. ¹H NMR, (400 MHz, CDCl₃): δ=6.08 (s, 1H), 4.06 (q, J=7.09, 2H), 3.43 (d, J=3.67, 1H), 3.36 (d, J=3.55, 1H), 2.53-1.25 (m, 19H), 1.19 (t, J=7.15, 3H), 1.11 (s, 3H), 1.03 (s, 3H), 0.81 (d, J=7.15, 3H). Mass (m/z): 429.2 (M⁺+1).
Step 11
To a pressure tube was added compound 22 (110.00 mg, 256.67 umol, 1.00 eq), Py (6.00 mL) and Pd/C (50.00 mg, 256.67 umol, 1.00 eq). The reaction was purged with H₂(50 psi) gas and stirred at 20° C. for 16 hrs. LCMS showed the reaction was completed. The reaction was filtered, the filtrate was concentrated in vacuo to give compound 23 (100 mg, crude) as yellow oil. Mass (m/z): 433.3 (M⁺+1).
Step 12
To a 35 ml flask was added compound 23 (100 mg, crude) and THF (10 ml). LiAlH(t-OBu)₃(117.09 mg, 462.33 umol, 130.10 uL, 2.00 eq) was added to the flask at 20° C., and it was stirred at 20° C. for 5 hours. After TLC (PE/EA=1/3) showed the reaction was completed, the reaction was quenched by saturated NH₄Cl (20 ml), diluted with EA (100 ml), and washed with water (50 ml). The organic layer was concentrated in vacuo to give compound 24 (100 mg, crude) as colorless oil. Mass (m/z): 459.3 (M⁺+23).
Step 13
To a 35 ml tube was added compound 24 (100 mg, crude) and EtOH (2 ml), followed by adding NaOH (36.64 mg, 916.12 umol, 4.00 eq) to the reaction at 30° C. The resulting mixture was stirred at 30° C. for 1 hour. After TLC (AcOH/EA=1/50) the desired product was found, the reaction was concentrated in vacuo to remove solvent, the residue was diluted with water (5 ml), acidized by conc. HCl to pH=3, and then extracted with EA (3*20 ml). The organic layer was concentrated in vacuo, the resulting residue was purified by pre-TLC (AcOH/EA=1/50) to give CA (15 mg) as white solid. ¹H NMR, (400 MHz, CDCl₃): δ=11.94 (br s, 1H), 4.33 (d, J=4.27, 1H), 4.13 (d, J=3.51, 1H), 4.02 (d, J=3.39, 1H), 3.79 (br d, J=2.76, 1H), 3.62 (br s, 1H), 3.22-3.16 (m, 1H), 2.27-2.02 (m, 5H), 1.78-1.44 (m, 6H), 1.47-1.13 (m, 11H), 1.01-0.96 (m, 1H), 0.93 (d, J=6.4, 3H), 0.88-0.84 (m, 1H), 0.81 (s, 3H), 0.59 (s, 3H). Mass (m/z): 817.6 (2M⁺+1).

Example 3

Synthesis of Cholic Acid from Ac-DHEA

Step 1
To a flask was added Ac-DHEA (50 g, 151.30 mmol, 1.0 eq) and toluene (1 L), 2-pyridylmethanamine (37.49 g, 346.72 mmol, 35.37 ml, 5.0 eq) was added to the reaction at 15° C., followed by adding PTSA (781.64 mg, 4.54 mmol, 0.03 eq) at 15° C. The reaction was heated to 110° C. with Dean-Stark apparatus for 2 hours. The reaction was cooled to 15° C., EA (1 L) was added to the reaction. The resulting mixture was washed with saturated NaHCO₃(2*100 ml) and water (2*100 ml). The organic layer was concentrated in vacuo. The residue was re-crystallized by EA (65 ml) to give compound 25 (54.0 g, 84.86% yield) as light yellow solid.
Step 2
To a 100 ml flask was added compound 25 (10 g, 23.78 mmol, 1.00 eq) sodium ascorbate (9.42 g, 47.55 mmol, 2.00 eq) and Cu(OTf)₂(19.78 g, 54.69 mmol, 2.30 eq) at 25° C. Acetone (90 mL) and MeOH (90 mL) was added to the flask to prepare a solution. After stirred at 25° C. for 5 mins, O₂(15 psi) was bubbled through the reaction mixture for 5 mins. Then, the reaction was heated to 50° C., and it was stirred at 50° C. for 1.5 hrs. TLC (PE/EA=2/1) showed most of starting material was consumed, and a main new spot was found. The reaction was cooled to 25° C. EA (100 ml) and saturated EDTA-Na₄(100 ml) was added to the reaction and stirred for 1 hour. The resulting mixture was concentrated in vacuo to remove organic solvent. The aqueous layer was extracted with EA (3*150 ml). The combined organic layer concentrated in vacuo. The residue was purified by flash column chromatography (PE/EA=20/1 to 2/1) to give compound 26 (6.2 g, 71.5% yield) as white solid. ¹H NMR, (400 MHz, CDCl₃): δ=5.43 (d, J=5.2, 1H), 4.66-4.59 (m, 1H), 3.87-3.79 (m, 1H), 3.08 (s, 1H), 2.50 (dd, J=8.8, 19.5 Hz, 1H), 2.46-2.37 (m, 2H), 2.15-2.05 (m, 2H), 2.05 (s, 3H), 2.03-2.01 (m, 1H), 1.91-1.85 (m, 3H), 1.71-1.60 (m, 3H), 1.52-1.30 (m, 1H), 1.29-1.13 (m, 3H), 1.08 (s, 3H), 0.98 (s, 3H).
Step 3
To a 250 ml tube was added EtPPh₃Br (26.63 g, 71.74 mmol, 5.00 eq) at 20° C., and it was purged with N2 gas. t-BuOK (8.05 g, 71.74 mmol, 5.00 eq) and THF (100 mL) was added to the flask at 20° C. The mixture was heated to 70° C., and it was stirred at 70° C. for 0.5 hr. Compound 26 (5 g, 14.35 mmol, 1.00 eq) was added to the reaction at 70° C., and it was stirred at 70° C. for another 2 hrs. TLC (PE/EA=2/1) showed all the starting material was consumed, and a new spot was formed. The reaction was diluted with EA (250 ml), quenched by water (100 ml) and separated. The aqueous layer was extracted with EA (2*50 ml). The combined organic layer was concentrated in vacuo to give 9 g of crude product as yellow oil.
To a 250 ml flask was added the yellow oil previous obtained (9 g, crude). DCM (75 mL) was added to the flask to prepare a solution. Ac2O (2.80 g, 27.46 mmol, 2.57 mL, 2 eq) was added to the flask at 20° C. DMAP (167.74 mg, 1.37 mmol, 0.1 eq) and Et₃N (5.56 g, 54.92 mmol, 7.64 mL, 4 eq) was added to the flask at 20° C. The reaction was stirred at 20° C. for 16 hrs. TLC (PE/EA=5/1) showed all the starting material was consumed, and a new spot was formed. The reaction was diluted with DCM (100 ml), washed with water (50 ml) and separated. The organic layer was concentrated in vacuo. The residue was purified by flash column chromatography (PE/EA=20/1 to 10/1) to give compound 15 (3.7 g, 66.9% yield), which can be converted into cholic acid by following the sequence described in Example 2.

Example 4

Synthesis of Deoxycholic Acid

To a 35 ml flask is added compound 19 and THF (10 ml). LiAlH(t-OBu)₃(2.00 eq) is added to the flask at 20° C., and it is stirred at 20° C. for 5 hours. After TLC (PE/EA=1/3) shows the reaction is completed, the reaction is quenched by saturated NH₄Cl (20 ml), diluted with EA (100 ml), and washed with water (50 ml). The organic layer is then concentrated in vacuo to give compound 27.
To a 35 ml flask is added compound 27 and EtOH (2 ml), followed by adding NaOH (4.00 eq) to the reaction at 30° C. The resulted mixture is stirred at 30° C. for 1 hour. After TLC (AcOH/EA=1/50) shows the desired product, the reaction is concentrated in vacuo to remove solvent, the residue is diluted with water (5 ml), pH is adjusted by conc. HCl to pH=3, and then is extracted with EA (3*20 ml). The organic layer is concentrated in vacuo, the resulted residue is purified by pre-TLC (AcOH/EA=1/50) to give deoxycholic acid.

Example 5

Synthesis of Chenodeoxycholic Acid

Step 1
Compound 4, which can be obtained from DHEA following the processes described in Example 1, is firstly converted into compound 28.
Specifically, EtPPh3Br (5.00 eq) in a flask is dried under high vacuum for 1 hr at 70° C. Dry THF (3.00 mL) is added, followed by adding t-BuOK (5.00 eq) under N₂. The entire mixture is still stirred at 70° C. for 0.5 hr under N₂. The solution of Compound 4 (1.00 eq) in dry THF (2.00 mL) is added to the reaction at 70° C., the reaction is stirred at 70° C. for another 2 hrs. TLC shows the starting material is all consumed and one new spot is formed. After cooling to room temperature, the solution is diluted by EA (40 mL), quenched by saturated NaCl solution (10 mL). The aqueous solution is extracted with EA (20 mL), dried over Na₂SO₄, concentrated in vacuum to give an intermediate product. The intermediate product (1.00 eq) is then dissolved in DCM (5.00 mL) at 25° C., followed by adding Ac₂O (4.2 eq), Et₃N (6.00 eq) and DMAP (0.20 eq) at 25° C. The reaction is stirred at 25° C. for 7 hrs. The reaction is diluted with EA (50 ml), washed with H₂O (10 mL), dried over Na₂SO₄and filtered, the organic layer is concentrated in vacuo. The residue is purified by column chromatography to give Compound 28.
Step 2
To a 50 ml flask is added compound 28 (1.00 eq) and DCM (5.00 mL). The reaction is cooled to 0° C.; paraformaldehyde (5.00 eq) is added to the reaction at 0° C., followed by adding BF₃.Et₂O (0.10 eq) to the reaction at 0° C. The reaction is warmed to 15° C., and stirred at 15° C. for 2 hrs. The reaction is diluted with DCM (50 ml), and quenched by water (30 ml). The organic layer is concentrated in vacuo. The residue is purified to give an intermediate product. To a 10 ml tube is added (COCl)₂(2.40 eq) and DCM (6.00 mL). The mixture is cooled to −78° C. DMSO (4.00 eq) is added drop-wise to the reaction mixture at −78° C. After addition, the reaction is stirred at −78° C. for 0.5 hrs. The intermediate product is then added to the reaction at −78° C. It is stirred at −78 for 0.5 hrs. TEA (10.00 eq) is added drop-wise at −78° C. After addition, the reaction is warmed to 15° C., and stirred at 15° C. for 0.5 hr. The reaction is diluted with DCM (50 ml), and quenched by water (30 ml). The separated organic layer is concentrated in vacuo. The residue is purified to give compound 29.
Step 3
To a 10 ml tube is added NaH (4.00 eq) and THF (2.00 mL), followed by adding ethyl 2-diethoxyphosphorylacetate (5.00 eq) to the flask at 15° C. The resulted mixture is stirred at 15° C. for 10 mins. Compound 29 (1.00 eq) is added to the flask at 15° C., and stirred at 15° C. for 0.5 hr. The reaction is then quenched by water (5 ml), and extracted with DCM (2*30 ml). The organic layer is concentrated in vacuo. The residue is purified to give compound 30.
Step 4
To a 35 ml pressure tube is added compound 30 (1.00 eq), EtOH (4.00 mL) and Pd/C (0.1 eq based on Pd) at 25° C. The mixture is purged with H₂gas (50 psi) and stirred at 25° C. for 4 hrs. The resulting mixture is filtered, the filtrate is concentrated in vacuo to give compound 31.
Step 5
To a solution of compound 31 (1.00 eq) in MeOH (4.00 mL) is added NaOH (12.00 eq) at 25° C. After that, the reaction is heated to 60° C., and stirred at 60° C. for 2 hrs. The reaction is than allowed to cool to rt. After it is concentrated in vacuo, the resulting residue is dissolved in water (5 ml), and the pH is adjusted by HCl to PH=3. The resulted aqueous layer is extracted with EA (3*15 ml). The organic layer is concentrated in vacuo to give Chenodeoxycholic acid.

Example 6

Characteristics of Synthetic Cholic Acid

A few process impurities (see e.g., impurities 1-4 in FIG. 2) in the final cholic acid produced in Example 1 or 2 have been isolated using preparative HPLC (e.g., Shimadzu LC-20AP prep HPLC system), with H₂O (0.1% TFA) and acetonitrile as eluents, Column: Luna C18 300*50 mm, 10u, ELSD as monitor and identified as Compounds 32, 33, 34, and 35 as follows. Those skilled in the art would know how to adjust the flow rate and gradients to obtain optimal separation. The purity of the isolated product can be analyzed by analytical HPLC, for example, also using H₂O (0.1% TFA) and acetonitrile as eluents, Column: Luna C18 100*30 mm, 5u.
The ¹H-NMR spectra below were obtained using Bruker AV 400 (400 MHz); and ¹³C NMR spectra below were obtained using Bruker AV 400 (100 MHz). In reporting the NMR spectra, the following conventions are used: s=singlet, d=doublet, t=triplet, m=multiplet, br s=broad singlet, ov=overlapped; a and b show the two different protons in prochiral methylene.
Compound 32 is a process impurity having the following structure:
Compound 32 can be derived, for example, from hydrogenation of a product formed in Step 8 (part a) of Example 1 followed by deprotection. Mass spectral analysis shows a peak at 733.5 (Positive Scan, 2M+1). The assignments of chemical shifts (in ppm) of proton and carbon are tabulated in Table 2-A.

TABLE 2A

Chemical Shift Assignments for
Compound 32 in DMSO-d⁶(25° C.)

Position	¹H NMR_(ppm)(nH, m)	¹³C NMR_(ppm)

1a	0.84, 1H, ov	35.3
1b	1.64, 1H, ov
2a	1.42, 1H, ov	30.4
2b	1.27, 1H, ov
3	3.18, 1H, tt	70.4
4a	1.45, 1H, ov	39.6
4b	2.23, 1H, ov
5	1.23, 1H, ov	41.5
6a	1.36, 1H, ov	34.9
6b	1.79, 1H, ov
7	3.60, 1H, br.s	66.3
8	1.32, 1H, ov	39.3
9	2.15, 1H, ov	26.3
10	—	34.4
11	1.40, 2H, ov	28.6
12	3.79, 1H, br.s	71.0
13	—	45.9
14	1.95, 1H, ov	41.1
15a	0.96, 1H, ov	23.0
15b	1.64, 1H, ov
16a	1.16, 1H, ov	27.0
16b	1.67, 1H, ov
17	1.78, 1H, ov	43.3
18	0.59, 3H, s	12.5
19	0.81, 3H, s	22.7
20	1.33, 1H, ov	39.4
21	0.98, 3H, d, 6.48 Hz	15.7
22a	3.01, 1H, m	65.9
22b	3.40, 1H, m
3-OH	4.32, 1H, d, 4.40 Hz	—
7-OH	4.00, 1H, d, 3.30 Hz	—
12-OH	4.09, 1H, d, 3.55 Hz	—
22-OH	4.22, 1H, t, 5.26 Hz	—

The ¹H-NMR spectrum of Compound 32 shows the structure characteristic of cholesteric acid derivative, which is one of steroids. All protons resonate in the aliphatic area of the spectrum and three methyl signals can be observed in the high field: 0.59 ppm (3H, s, H-18), 0.81 ppm (3H, s, H-19) and 0.98 ppm (3H, d, J=6.48 Hz, H-21). In the lower field of the aliphatic area, three methines adjacent to the oxygen atom can be found: 3.79 ppm (1H, m, H-12), 3.60 ppm (1H, br.s, H-7) and 3.18 ppm (1H, m, H-3). Meanwhile, one methylene adjacent to the oxygen atom, which is a group in the C17 side chain of steroids skeleton, resonate at 3.01 ppm (1H, m) and 3.40 ppm (1H, m) in the lower field area.
In the COSY spectrum, the methylene at 3.01 ppm and 3.40 ppm correlate with the methine at 1.33 ppm (H-20) and active proton at 4.22 ppm (1H, t, J=5.26 Hz). And that methine at 1.33 ppm also correlate with the methyl at 0.98 ppm (H-21) and methine at 1.78 ppm (H-17). These signals prove that the C17 side chain is ‘1-hydroxypropan-2-yl’. In the HMBC spectrum, H-19 correlate with C-1, C-10, C-5 and C-9; H-18 correlate with C-12, C-13, C-14 and C-17; H-21 correlate with C-20, C-22 and C-17. Those correlation signals support the correct attachment of several groups. In the NOESY spectrum, the relative configuration of A/B ring is ‘cis’ because of the fact that methyl at 0.81 ppm (H-19) correlate with methine at 1.23 ppm (H-5); B/C ring's is ‘trans’ as methyl H-19 correlated with methine at 1.32 (H-8) and don't correlate with methine at 2.14 ppm (H-9);C/D ring's is ‘trans’ as methyl at 0.59 ppm (H-18) don't correlate with methine at 1.95 ppm (H-14) and correlate with H-8; the relative configuration of C-17 is ‘β’ because methyl at 0.59 ppm correlate with methine at 1.33 ppm (H-20) and don't correlate with methine at 1.78 ppm (H-17); The correlation signals between methine at 3.18 ppm (H-3) and H-5, methine at 3.60 ppm (H-7) and H-8/H-6(1.36 ppm and 1.78 ppm), methine at 3.79 ppm and H-19 can support the conclusion of 3α(OH), 7α(OH) and 12α(OH). However, the configuration of C-20 cannot be ensured based on the NMR studies because the chiral center is outside the ring. In conclusion, we can determine that the configuration of Compound 32 is 3α, 5β,7α,8β,9α,10β,12α,13β,14α,17β,20(R or S).
Compound 33 is a process impurity having the following structure:
Compound 33 is a diastereomer of cholic acid at the C20 position, which can be derived, for example, from a minor isomer formed in Step 8 (or epimerization) of Example 1 that carried through the synthesis until cholic acid. Mass spectral analysis shows a peak at 817.6 (Positive Scan, 2M+1). The assignments of chemical shifts (in ppm) of proton and carbon are tabulated in Table 2-B:

TABLE 2B

Chemical Shift Assignments for
Compound 33 in DMSO-d6 (25° C.)

Position	¹H NMR_(ppm)(nH, m)	¹³C NMR_(ppm)

1a	0.83, 1H, ov	35.3
1b	1.64, 1H, ov
2a	1.41, 1H, ov	30.3
2b	1.28, 1H, ov
3	3.18, 1H, tt	70.5
4a	1.45, 1H, ov	39.6
4b	2.21, 1H, ov
5	1.23, 1H, ov	41.5
6a	1.36, 1H, ov	34.9
6b	1.79, 1H, ov
7	3.61, 1H, br.s	68.3
8	1.33, 1H, ov	39.5
9	2.14, 1H, ov	26.2
10	—	34.6
11a	1.44, 1H, ov	28.5
11b	1.38, 1H, ov
12	3.80, 1H, br.s	71.0
13	—	45.7
14	1.99, 1H, ov	41.3
15a	0.96, 1H, ov	22.7
15b	1.65, 1H, ov
16a	1.15, 1H, ov	27.4
16b	1.67, 1H, ov
17	1.78, 1H, ov	46.3
18	0.59, 3H, s	12.5
19	0.80, 3H, s	22.6
20	1.34, 1H, ov	34.4
21	0.78, 3H, d, 5.50 Hz	18.3
22a	1.87, 1H, ov	29.3
22b	1.36, 1H, ov
23a	2.35, 1H, ov	31.1
23b	2.09, 1H, ov
24	—	175.2

The ¹H-NMR spectrum of Compound 33 shows the structure characteristic of cholesteric acid derivative, which is one of steroids. All protons resonate in the aliphatic area of the spectrum and three methyl signals can be observed in the high field: 0.59 ppm (3H, s, H-18), 0.80 ppm (3H, s, H-19) and 0.78 ppm (3H, d, J=5.50 Hz, H-21). In the lower field of the aliphatic area, three methines adjacent to the oxygen atom can be found: 3.80 ppm (1H, br.s, H-12), 3.61 ppm (1H, br.s, H-7) and 3.18 ppm (1H, tt, J₁=10.3 Hz, J₂=4.0 Hz, H-3).
In the COSY spectrum, the methyl at 0.78 ppm (H-21) correlate with the methine at 1.34 ppm (H-20) and H-20 correlate with methine at 1.79 ppm (H-17). According to the HMBC spectrum, H-21 correlate with carbon at 46.3 ppm (C-17), 39.3 ppm (C-22). The two methylenes H22 and H23 correlate with carbonyl at 175.2 ppm (C-24). These signals prove that the C17 side chain is ‘1-n-valeric acid-4-yl’.
In the HMBC spectrum, H-19 correlate with C-1, C-5, C-9 and C-10; H-18 correlate with C-12, C-13, C-14 and C-17. Those correlation signals support the correct attachment of several groups.
In the NOESY spectrum, the relative configuration of A/B ring is ‘cis’ because of the fact that methyl at 0.80 ppm (H-19) correlate with methine at 1.23 ppm (H-5); B/C ring's is ‘trans’ as methyl H-19 correlate with methine at 1.33 (H-8) and don't correlate with methine at 2.14 ppm (H-9);C/D ring's is ‘trans’ as methyl at 0.59 ppm (H-18) don't correlate with methine at 1.99 ppm (H-14) and correlate with H-8; the relative configuration of C-17 is ‘β’ because methyl at 0.59 ppm correlate with methine at 1.34 ppm (H-20) and don't correlate with methine at 1.78 ppm (H-17); The correlation signals between methine at 3.18 ppm (H-3) and H-5, methine at 3.61 ppm (H-7) and H-8, H-6(1.36 ppm and 1.79 ppm), methine at 3.80 ppm and H-18 can support the conclusion of 3α(OH), 7α(OH) and 12α(OH). However, the configuration of C-20 cannot be ensured based on NMR because the chiral center is outside the ring. In conclusion, we can determine that the configuration is 3α, 5β,7α,8β,9α,10β,12β,13β,14α,17β,20(R or S). As Compound 33 has the same stereochemistry at the 3, 5, 7, 8, 9, 10, 12, 13, 14, and 17 position as that of cholic acid, the C20 stereochemistry of Compound 33 is assigned as “S”, as cholic acid has a “R” configuration at C20.
Compound 34 is a process impurity having the following structure:
Compound 34 is a diastereomer of cholic acid at the C17 position, which can be derived, for example, from a minor isomer formed in Step 10 of Example 1 that carried through the synthesis until cholic acid. Mass spectral analysis shows a peak at 407.3 (Negative Scan, M−1). The assignments of chemical shifts (in ppm) of proton and carbon are tabulated in Table 2-C:

TABLE 2C

Chemical Shift Assignments for Compound
34 in chloroform -d (25° C.)

Position	H NMR_(ppm)(nH, m)	¹³C NMR_(ppm)

1a	0.97, 1H, ov	35.4
1b	1.74, 1H, ov
2a	1.40, 1H, ov	29.9
2b	1.67, 1H, ov
3	3.47, 1H, m	71.9
4a	1.78, 1H, ov	39.4
4b	2.22, 1H, ov
5	1.39, 1H, ov	41.4
6a	1.57, 1H, ov	34.4
6b	1.92, 1H, ov
7	3.94, 1H, br.s	69.3
8	1.62, 1H, ov	39.2
9	2.27, 1H, ov	26.4
10	—	34.8
11	1.59, 2H, ov	29.7
12	4.27, 1H, br.s	73.3
13	—	47.1
14	2.00, 1H, ov	40.0
15a	1.10, 1H, ov	25.2
15b	1.71, 1H, ov
16a	1.28, 1H, ov	29.4
16b	1.84, 1H, ov
17	1.40, 1H, ov	55.9
18	0.78, 3H, s	24.2
19	0.88, 3H, s	22.3
20	1.90, 1H, ov	34.7
21	0.80, 3H, d, 6.36 Hz	18.1
22a	1.15, 1H, ov	31.9
22b	2.04, 1H, ov
23	2.43, 2H, m	32.1
24	—	178.5

The ¹H-NMR spectrum of Compound 34 shows the structure characteristic of cholesteric acid derivative, which is one of steroids. All protons resonate in the aliphatic area of the spectrum and three methyl signals can be observed in the high field: 0.77 ppm (3H, s, H-18), 0.88 ppm (3H, s, H-19) and 0.80 ppm (3H, d, J=6.48 Hz, H-21). In the lower field of the aliphatic area, three methines adjacent to the oxygen atom can be found: 4.27 ppm (1H, br.s, H-12), 3.94 ppm (1H, br.s, H-7) and 3.47 ppm (1H, m, H-3).
In the COSY spectrum, the methyl at 0.80 ppm (H-21) correlate with the methine at 1.90 ppm (H-20) and H-20 correlate with methine at 1.40 ppm (H-17). According to the HMBC spectrum, H-21 correlate with carbon at 55.9 ppm (C-17), 31.9 ppm (C-22). The two methylenes H22 and H23 correlate with carbonyl at 178.5 ppm (C-24). These signals prove that the C17 side chain is ‘1-n-valeric acid-4-yl’.
In the HMBC spectrum, H-18 correlate with C-1, C-10, C-5 and C-9; H-19 correlate with C-12, C-13, C-14 and C-17; H-21 correlate with C-20, C-22 and C-17. Those correlation signals support the correct attachment of several groups.
In the NOESY spectrum, the relative configuration of A/B ring is ‘cis’ because of the fact that methyl at 0.88 ppm (H-19) correlate with methine at 1.39 ppm (H-5); B/C ring's is ‘trans’ as methyl H-19 correlate with methine at 1.62 (H-8) and don't correlate with methine at 2.27 ppm (H-9);C/D ring's is ‘trans’ as methyl at 0.78 ppm (H-18) don't correlate with methine at 2.00 ppm (H-14) and correlate with H-8; the relative configuration of C-17 is ‘a’ because methyl at 0.78 ppm correlate with methine at 1.39 ppm (H-17) and don't correlate with methine at 1.90 ppm (H-20); The correlation signals between H-3 and H-5, H-7 and H-8 H-6(1.57 ppm and 1.92 ppm), H-12 and H-18 can support the conclusion of 3α(OH), 7α(OH) and 12α(OH).
However, the configuration of C-20 cannot be ensured based on the NMR studies because the chiral center is outside the ring. In conclusion, we can determine that the configuration is 3α, 5β,7α,8β,9α,10β,12α,13β,14α,17α,20(R or S). Nevertheless, as Compound 34 is a diastereomer of Compound 35 (see below) with the only difference being the C20 stereochemistry, and X-ray crystal structure of Compound 35 shows that it has a C20 S configuration, the stereochemistry at C20 of Compound 34 is assigned as R configuration.
Compound 35 is a process impurity having the following structure:
Compound 35 is a diastereomer of Compound 34 at the C20 position, which can be derived, for example, from a minor isomer formed in Steps 8-10 of Example 1 that carried through the synthesis until cholic acid. Mass spectral analysis shows a peak at 407.2 (Negative Scan, M−1). The assignments of chemical shifts (in ppm) of proton and carbon are tabulated in Table 2-D:

TABLE 2D

Chemical Shift Assignments for Compound
35 in chloroform -d (25° C.)

Position	¹H NMR_(ppm)(nH, m)	¹³C NMR_(ppm)

1a	0.97, 1H, ov	34.3
1b	1.75, 1H, ov
2a	1.37, 1H, ov	29.7
2b	1.66, 1H, ov
3	3.48, 1H, tt	71.9
4a	1.76, 1H, ov	39.1
4b	2.13, 1H, ov
5	1.41, 1H, ov	41.3
6a	1.57, 1H, ov	34.3
6b	1.94, 1H, ov
7	3.94, 1H, br.s	69.2
8	1.61, 1H, ov	39.8
9	2.25, 1H, ov	26.3
10	—	34.8
11a	1.56, 1H, ov	29.6
11b	1.65, 1H, ov
12	4.06, 1H, br.s	73.1
13	—	48.0
14	2.01, 1H, ov	39.5
15a	1.16, 1H, ov	25.3
15b	1.80, 1H, ov
16a	1.43, 1H, ov	25.6
16b	1.82, 1H, ov
17	1.57, 1H, ov	55.7
18	0.75, 3H, s	23.8
19	0.89, 3H, s	22.2
20	1.99, 1H, ov	34.2
21	0.91, 3H, d, 6.48 Hz	20.5
22a	1.08, 1H, ov	29.5
22b	1.98, 1H, ov
23	2.36, 2H, m	32.4
24	—	178.5

The ¹H-NMR spectrum of Compound 35 shows the structure characteristic of cholesteric acid derivative, which is one of steroids. All protons resonate in the aliphatic area of the spectrum and three methyl signals can be observed in the high field: 0.75 ppm (3H, s, H-18), 0.89 ppm (3H, s, H-19) and 0.91 ppm (3H, d, J=6.48 Hz, H-21). In the lower field of the aliphatic area, three methines adjacent to the oxygen atom can be found: 4.06 ppm (1H, br.s, H-12), 3.94 ppm (1H, br.s, H-7) and 3.48 ppm (1H, m, H-3).
In the COSY spectrum, the methyl at 0.91 ppm (H-21) correlate with the methine at 1.99 ppm (H-20) and H-20 correlate with methine at 1.57 ppm (H-17). According to the HMBC spectrum, H-21 correlate with carbon at 55.7 ppm (C-17), 29.5 ppm (C-22). The two methylenes H22 and H23 correlate with carbonyl at 178.5 ppm (C-24). These signals prove that the C17 side chain is ‘1-n-valeric acid-4-yl’.
In the HMBC spectrum, H-18 correlate with C-1, C-10, C-5 and C-9; H-19 correlate with C-12, C-13, C-14 and C-17; H-21 correlate with C-20, C-22 and C-17. Those correlation signals support the correct attachment of several groups.
In the NOESY spectrum, the relative configuration of A/B ring is ‘cis’ because of the fact that methyl at 0.89 ppm (H-19) correlate with methine at 1.41 ppm (H-5); B/C ring's is ‘trans’ as methyl H-19 correlate with methine at 1.61 (H-8) and don't correlate with methine at 2.25 ppm (H-9);C/D ring's is ‘trans’ as methyl at 0.75 ppm (H-18) don't correlate with methine at 2.01 ppm (H-14) and correlate with H-8; the relative configuration of C-17 is ‘α’ because methyl at 0.75 ppm correlate with methine at 1.57 ppm (H-17) and don't correlate with methine at 1.99 ppm (H-20); The correlation signals between H-3 and H-5, H-7 and H-8 H-6(1.57 ppm and 1.92 ppm), H-12 and H-18 can support the conclusion of 3α(OH), 7α(OH) and 12α(OH).
However, the configuration of C-20 cannot be ensured based on NMR studies because the chiral center is outside the ring. In conclusion, we can determine that the configuration of Compound 35 is 3α,5β,7α,8β,9α,10β,12α,13β,14α,17α,20(R or S).
Nonetheless, an X-ray structure of a crystal of Compound 35 was obtained, which concludes that the stereochemistry of Compound 35 at C20 position is in the S configuration. See FIG. 1.
The X-ray structural analysis: A sample of Compound 35 was dissolved in acetone and kept in a half sealed vial. The solvent evaporate slowly at room temperature. A crystal suitable in size was obtained in the second day. Its transparency was check by microscope and then the crystal was sent for X-ray test using Rigaku Saturn Diffractometer using graphic-monochromated Mo Kα radiation (λ=0.71073 Å). The following parameter was used: Radiation monochromator: artificial multimembrane focusing mirror; Single tube diameter: ϕ=0.50 mm; Distance from the crystal to the CCD detector: d=45 mm; Tube Pressure: 50 kV; Tube Flow: 16 mA. A total of 27071 reflections were collected in the theta range from 2.303 to 31.246°. The limiting indices were: −12≤h≤12, −12≤k≤12, −42≤1≤43; which yielded 7098 unique reflections (R_int=0.0650). The structure was solved using direct methods and was refined by full-matrix least-squares on F2 values. Non-hydrogen atoms were refined anisotropically. Hydrogen atoms were fixed at calculated positions and refined using a riding mode. The total number of refined parameters was 310, compared with 7098 data. All reflections were included in the refinement. The goodness of fit on F2 was 1.031 with a final R value for [I>2σ(I)] R=0.0668 and R_w=0.1664. The largest differential peak and hole were 0.391 and −0.413 e. Å⁻³, respectively.
The crystal was a colorless prism with the following dimensions: 0.20×0.18×0.12 mm³. The symmetry of the crystal structure was assigned the orthorhombic space group P2(1)2(1)2(1) with the following parameters: a=8.4747(4) Å, b=9.2565(4) Å, c=29.9531(11) Å, α=β=γ=90°, V=2349.7(17) Å³, Z=4, Dc=1.240 Mg/m³, F(000)=968, μ(Mo Kα)=0.088 mm⁻¹, and T=113(2) K. See Table 3 below.

TABLE 3

Summary of X-ray Crystallographic Data of Compound 35

	Crystal Size	0.20 × 0.18 × 0.12 mm³
	Radiation Type	Mo Kα (λ = 0.71073 Å)
	Space Group	Orthorhombic
		P2(1)2(1)2(1)
	Cell Size	a = 8.4747(4) Å
		b = 9.2565(4) Å
		c = 29.9531(11) Å
		α = 90°
		β = 90°
		γ = 90°
	Cell Volume	V = 2349.70(17) Å³
	Cell Formula Units	Z = 4
	Crystal Density	D_c= 1.240 Mg/m³
	Crystal F(000)	968
	Absorption Coefficient mu	μ(Mo Kα) = 0.088 mm⁻¹
	Limiting Indices	−12 ≤ h ≤ 12
		−12 ≤ k ≤ 12
		−42 ≤ 1 ≤ 43
	Cell Measurement Temperature	T = 113(2) K.
	Theta range for data collection	2.303 to 31.246°
	Goodness-of-fit on F{circumflex over ( )}2	1.031
	Final R indices [I > 2sigma(I)]	R1 = 0.0668, wR2 = 0.1664
	R indices (all data)	R1 = 0.0877, wR2 = 0.1841
	Largest diff. peak and hole	0.391 and −0.413 e.Å⁻³
	Reflections collected/unique	27071/7098 [R_(int)= 0.0650]

With respect to aspects of the invention described as a genus, all individual species are individually considered separate aspects of the invention. If aspects of the invention are described as “comprising” a feature, embodiments also are contemplated “consisting of or “consisting essentially of” the feature.
The foregoing description of the specific embodiments will so fully reveal the general nature of the invention that others can, by applying knowledge within the skill of the art, readily modify and/or adapt for various applications such specific embodiments, without undue experimentation, without departing from the general concept of the present disclosure. Therefore, such adaptations and modifications are intended to be within the meaning and range of equivalents of the disclosed embodiments, based on the teaching and guidance presented herein. It is to be understood that the phraseology or terminology herein is for the purpose of description and not of limitation, such that the terminology or phraseology of the present specification is to be interpreted by the skilled artisan in light of the teachings and guidance.
The breadth and scope of the present disclosure should not be limited by any of the above-described exemplary embodiments, but should be defined only in accordance with the following claims and their equivalents.
All of the various aspects, embodiments, and options described herein can be combined in any and all variations.
All publications, patents, and patent applications mentioned in this specification are herein incorporated by reference to the same extent as if each individual publication, patent, or patent application was specifically and individually indicated to be incorporated by reference.

Claims

1.-76. (canceled)

77. A compound having a structure of

wherein R³¹, R³²and R³³are each independently H or a hydroxyl protecting group.

78. The compound of claim 77, wherein R³²is H, and R³¹and R³³are each independently a hydroxyl protecting group.

79. The compound of claim 78, wherein the hydroxyl protecting group is acetyl.

80. A method of preparing a compound of Formula III-8

wherein R³¹, R³²and R³³are each independently H or a hydroxyl protecting group,

the method comprising

a) contacting a diketone of Formula III-11a with a ketone reducing agent to form a compound of Formula III-12a

b) reacting the compound of Formula III-12a with an amine to form an imine of Formula III-13a

wherein n is 0, 1 or 2, and R³⁴is C_1-6alkyl; and,

c) oxidizing the imine of Formula III-13a with O₂in the presence of a copper salt to form the compound of Formula III-8.

81. The method of claim 80, wherein the diketone of Formula III-11a is a compound of Formula III-11

and is obtained from the method of preparing the compound of Formula III-11,

the method comprising

a) reacting the compound of Formula III-9 with an epoxide forming reagent to form an epoxide of Formula III-10

b) reducing the epoxide of Formula III-10 under hydrogenation condition to form the compound of Formula III-11.

82. The method of claim 80, wherein R³²is H, and R³¹and R³³are each independently a hydroxyl protecting group.

83. The method of claim 82, wherein the hydroxyl protecting group is acetyl.