US20210069190A1 - Pharmaceutical composition comprising small molecule egfr inhibitor and preparation method therefor - Google Patents

Pharmaceutical composition comprising small molecule egfr inhibitor and preparation method therefor Download PDF

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US20210069190A1
US20210069190A1 US17/053,605 US201917053605A US2021069190A1 US 20210069190 A1 US20210069190 A1 US 20210069190A1 US 201917053605 A US201917053605 A US 201917053605A US 2021069190 A1 US2021069190 A1 US 2021069190A1
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Prior art keywords
pharmaceutical composition
composition according
amount
microcrystalline cellulose
present
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US17/053,605
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Weiqi Chen
Junjun ZHAO
Xiaolei Wang
Baoqing YIN
Xiao Chen
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Jiangsu Hansoh Pharmaceutical Group Co Ltd
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Jiangsu Hansoh Pharmaceutical Group Co Ltd
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Assigned to JIANGSU HANSOH PHARMACEUTICAL GROUP CO., LTD. reassignment JIANGSU HANSOH PHARMACEUTICAL GROUP CO., LTD. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: CHEN, Weiqi, CHEN, XIAO, WANG, XIAOLEI, YIN, Baoqing, ZHAO, Junjun
Publication of US20210069190A1 publication Critical patent/US20210069190A1/en
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/20—Pills, tablets, discs, rods
    • A61K9/2004—Excipients; Inactive ingredients
    • A61K9/2013—Organic compounds, e.g. phospholipids, fats
    • A61K9/2018—Sugars, or sugar alcohols, e.g. lactose, mannitol; Derivatives thereof, e.g. polysorbates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
    • A61K31/506—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim not condensed and containing further heterocyclic rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/02—Inorganic compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
    • A61K47/10—Alcohols; Phenols; Salts thereof, e.g. glycerol; Polyethylene glycols [PEG]; Poloxamers; PEG/POE alkyl ethers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
    • A61K47/12—Carboxylic acids; Salts or anhydrides thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/32—Macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. carbomers, poly(meth)acrylates, or polyvinyl pyrrolidone
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
    • A61K47/38—Cellulose; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
    • A61K9/1682—Processes
    • A61K9/1694—Processes resulting in granules or microspheres of the matrix type containing more than 5% of excipient
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/20—Pills, tablets, discs, rods
    • A61K9/2004—Excipients; Inactive ingredients
    • A61K9/2009—Inorganic compounds
    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/20—Pills, tablets, discs, rods
    • A61K9/2004—Excipients; Inactive ingredients
    • A61K9/2013—Organic compounds, e.g. phospholipids, fats
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K9/20—Pills, tablets, discs, rods
    • A61K9/2004—Excipients; Inactive ingredients
    • A61K9/2022—Organic macromolecular compounds
    • A61K9/2027—Organic macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyvinyl pyrrolidone, poly(meth)acrylates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/20—Pills, tablets, discs, rods
    • A61K9/2004—Excipients; Inactive ingredients
    • A61K9/2022—Organic macromolecular compounds
    • A61K9/205—Polysaccharides, e.g. alginate, gums; Cyclodextrin
    • A61K9/2054—Cellulose; Cellulose derivatives, e.g. hydroxypropyl methylcellulose
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/20—Pills, tablets, discs, rods
    • A61K9/2004—Excipients; Inactive ingredients
    • A61K9/2022—Organic macromolecular compounds
    • A61K9/205—Polysaccharides, e.g. alginate, gums; Cyclodextrin
    • A61K9/2059—Starch, including chemically or physically modified derivatives; Amylose; Amylopectin; Dextrin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/20—Pills, tablets, discs, rods
    • A61K9/2095—Tabletting processes; Dosage units made by direct compression of powders or specially processed granules, by eliminating solvents, by melt-extrusion, by injection molding, by 3D printing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/20—Pills, tablets, discs, rods
    • A61K9/28—Dragees; Coated pills or tablets, e.g. with film or compression coating
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents

Definitions

  • the present invention belongs to the field of pharmaceutical formulations, and specifically relates to a pharmaceutical composition comprising a third-generation small molecule EGFR inhibitor and a method for preparing the same.
  • EGFR Epidermal Growth Factor Receptor
  • erbB receptor family which includes transmembrane protein tyrosine kinase receptors.
  • ligand such as epidermal growth factor (EGF)
  • EGFR can form a homodimer on the cell membrane or form a heterodimer with other receptors in the family, such as erbB2, erbB3, or erbB4.
  • the formation of these dimers can cause the phosphorylation of key tyrosine residues in EGFR cells, thereby activating a number of downstream signaling pathways in cells.
  • These intracellular signaling pathways play an important role in cell proliferation, survival and anti-apoptosis.
  • EGFR signal transduction pathways including increased expression of ligands and receptors, EGFR gene amplification and mutation and the like, can promote malignant transformation of cells, and play an important role in tumor cell proliferation, invasion, metastasis and angiogenesis. Therefore, EGFR is a reasonable target for the development of anticancer drugs.
  • N-(5-((4-(1-cyclopropyl-TH-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide which belongs to the third-generation small molecule EGFR inhibitor, has high selectivity for inhibiting the EGFR T790M mutant, while has no or low activity on wild-type EGFR, therefore, it can be used in treating drug-resistant tumors caused by the secondary mutation of EGFR-T790M.
  • This compound (hereinafter referred to as the compound of formula I) was first disclosed in the international patent application WO2016054987, and its structure is shown in the following formula I
  • the present invention provides a pharmaceutical composition comprising a third-generation small molecule EGFR inhibitor.
  • the pharmaceutical composition comprises a mesylate salt of N-(5-((4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide, a polymorph, solvate, hydrate, pharmaceutically acceptable salt thereof or a combination thereof as the active ingredient, and at least one pharmaceutically acceptable excipient.
  • the active ingredient is present in an amount of 1 to 60%.
  • the unit dose of the active ingredient is 10 to 200 mg, preferably 55 to 110 mg, and more preferably 55 mg or 110 mg.
  • the excipient comprises one or more filler(s), comprising at least one disaccharide or polysaccharide, such as glucan, starch, cellulose, lactose, maltose or sucrose.
  • the starch can be selected from the group consisting of potato starch, corn starch, rice starch, wheat starch, amylopectin, pregelatinized starch and the like.
  • the disaccharide or polysaccharide is present in an amount of 1 to 60%, preferably 5 to 55%, more preferably 5 to 30%, and further preferably 5 to 15%.
  • the filler is preferably a disaccharide, such as lactose, and it is present in an amount of 1 to 60%, preferably 5 to 55%, more preferably 5 to 30%, and further preferably 5 to 15%.
  • the excipient comprises one or more filler(s), disintegrant(s) or lubricant(s).
  • the weight percentage of each component is as follows:
  • the weight percentage of each component is as follows:
  • active ingredient 35 to 50% filler 30 to 50% disintegrant 10 to 20% lubricant 0.5 to 5%.
  • the weight percentage of each component is as follows:
  • the filler comprises one or more of microcrystalline cellulose, lactose, anhydrous lactose, corn starch, pregelatinized starch, mannitol, sorbitol, calcium hydrophosphate and calcium sulfate, wherein the filler is present in an amount of 20 to 80%, and preferably 30 to 50%.
  • the filler comprises two of the above components, and the weight ratio of the two components is 1:3 to 3:1.
  • the filler is preferably microcrystalline cellulose and lactose.
  • the microcrystalline cellulose is present in an amount of 1 to 60%, preferably 10 to 40%, and more preferably 20 to 40%, relative to the weight of the tablet core; and the lactose is present in an amount of 1 to 60%, preferably 5 to 30%, and more preferably 5 to 15%, relative to the weight of the tablet core.
  • Optional technical solutions include, but are not limited to, the microcrystalline cellulose is present in an amount of 1 to 60%, and the lactose is present in an amount of 1 to 60%; the microcrystalline cellulose is present in an amount of 10 to 40%, and the lactose is present in an amount of 5 to 30%; and the microcrystalline cellulose is present in an amount of 20 to 40%, and the lactose is present in an amount of 5 to 15%.
  • the weight ratio of microcrystalline cellulose to lactose is 1:3 to 3:1, and more preferably 2-3:1.
  • the lactose is anhydrous lactose.
  • the microcrystalline cellulose is present in an amount of 1 to 60%, preferably 10 to 40%, and more preferably 20 to 40%, relative to the weight of the tablet core; and the anhydrous lactose is present in an amount of 1 to 60%, preferably 5 to 30%, and more preferably 5 to 15%, relative to the weight of the tablet core.
  • Optional technical solutions include, but are not limited to, the microcrystalline cellulose is present in an amount of 1 to 60%, and the anhydrous lactose is present in an amount of 1 to 60%; the microcrystalline cellulose is present in an amount of 10 to 40%, and the anhydrous lactose is present in an amount of 5 to 30%; and the microcrystalline cellulose is present in an amount of 20 to 40%, and the anhydrous lactose is present in an amount of 5 to 15%.
  • the weight ratio of microcrystalline cellulose to anhydrous lactose is 1:3 to 3:1, more preferably 2-3:1.
  • the pharmaceutical composition with a high content of the compound of formula I can be prepared, and its drug loading capacity can reach more than 40%. At the same time, the resulting pharmaceutical composition has a good compressibility and uniform dissolution.
  • the disintegrant comprises one or more of low-substituted hydroxypropyl cellulose, croscarmellose sodium, carboxymethyl starch sodium and crospovidone, and preferably carboxymethyl starch sodium.
  • the disintegrant is present in an amount of 1 to 30%, and preferably 10 to 20%.
  • the disintegrant that is added in a totally intragranular manner can ensure good disintegration and preparation of the tablet.
  • the addition of carboxymethyl starch sodium can further improve the stability of the formulation.
  • the disintegrant is added intragranularly.
  • the lubricant comprises one or more of talc, stearic acid, sodium stearyl fumarate, glyceryl behenate, magnesium stearate and micronized silica gel.
  • the lubricant is present in an amount of 0.1 to 10%, and preferably 0.2 to 5%, relative to the weight of the tablet core.
  • the lubricant is preferably sodium stearyl fumarate and magnesium stearate.
  • the sodium stearyl fumarate is present in an amount of 0.1 to 5%, and preferably 0.3 to 3%
  • the magnesium stearate is present in an amount of 0.1 to 5%, and preferably 0.2 to 2%.
  • the combination of sodium stearyl fumarate and magnesium stearate can obviously improve the adhesion of raw materials on the surface of the equipment, ensure a smooth preparation process, and enable the finished product to have an excellent dissolution effect.
  • the amount refers to the percentage relative to the total weight of the drug, wherein the total weight of the drug does not include the weight of the coating.
  • the amount refers to the percentage relative to the weight of the tablet core or granule.
  • composition according to the present invention comprises the following components:
  • active ingredient 43.3% lactose 10.2% microcrystalline cellulose 30.0% carboxymethyl starch sodium 10.4% lubricant 0.5 to 5%.
  • active ingredient 43.3% lactose 10.2% microcrystalline cellulose 30.0% carboxymethyl starch sodium 14% lubricant 2.5%.
  • lactose 10.2% microcrystalline cellulose 30.0% carboxymethyl starch sodium 14% magnesium stearate 1% sodium stearyl fumarate 1.5%.
  • the pharmaceutical composition of the present invention is an oral formulation selected from the group consisting of tablet and capsule, preferably a film, and more preferably an immediate-release film-coated tablet.
  • the coating agent is a gastric-soluble film coating premix, preferably Opadry 85F140124 (the main ingredients are polyvinyl alcohol, polyethylene glycol, talc, titanium dioxide, iron oxide red and iron oxide black), and Opadry 85F12300 (the main ingredients are polyvinyl alcohol, polyethylene glycol, talc, titanium dioxide and iron oxide yellow).
  • the present invention further provides a method for preparing the pharmaceutical composition, mainly comprising the following steps of:
  • tableting compressing the resulting mixture into tablets according to the theoretical tablet weight
  • the above method comprises the specific steps of:
  • tableting compressing the resulting mixture into tablets according to the theoretical tablet weight
  • coating optionally, coating: i) formulation of a coating liquid, adding a prescription amount of Opadry to purified water under stirring to formulate a coating liquid with a solid content of 10%, stirring the coating liquid for 60 minutes after the completion of the addition of Opadry to make it evenly dispersed, and sieving the formulated coating liquid with a 100-mesh sieve for later use; and ii) setting the parameters according to the process requirements, and finishing the coating until the coating weight gain reaches about 2.0% to 4.0%.
  • N-(5-((4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide is a drug with low permeability. Its solubility is greatly affected by pH, which is pH dependent.
  • the pharmaceutical composition prepared according to the present invention has a good and uniform dissolution in different dissolution media.
  • the drug loading capacity of the active ingredient in the tablet was further increased.
  • the tablet still had a very good compressibility. There was no punch sticking.
  • the resulting uncoated tablets were smooth and 100% qualified, having a complete shape and good appearance.
  • the hardness of uncoated tablet is also one of the factors that affect the dissolution rate. Uneven hardness may lead to uneven dissolution, thereby affecting the efficacy.
  • Table 3 below shows the corresponding dissolution profile of the prescription of the present invention at different hardnesses. It can be seen from the table that the prescription of the present invention can eliminate the effect of hardness changes on the dissolution result of the uncoated tablet under different hardness conditions, and enable the product to maintain a good dissolution rate. Therefore, the prescription of the present invention can overcome the adverse effect caused by the different hardness of uncoated tablets.
  • the addition of the disintegrant can also affect the dissolution rate of the tablet.
  • Carboxymethyl starch sodium used as the disintegrant can ensure a good disintegration and preparation of the tablet, and the preferred dosage is 10% to 20%.
  • a pharmaceutical composition comprising the mesylate salt of N-(5+(4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide comprises the components of: 64.9 mg of the active ingredient (calculated by C 30 H 35 N 7 O 2 .CH 3 SO 3 H), 45.0 mg of microcrystalline cellulose (KG802), 15.3 mg of anhydrous lactose (21AN), 21.0 mg of carboxymethyl starch sodium (Type A), 0.8 mg of intragranular sodium stearyl fumarate, 1.5 mg of extragranular sodium stearyl fumarate and 1.5 mg of magnesium stearate.
  • the prescription is as follows:
  • the immediate-release film-coated tablets comprising the above components were prepared by the following preparation method:
  • the raw materials were weighed according to the schedule list.
  • Premixing I the intragranular raw materials were mixed in a hopper mixer (speed: 15 rpm, time: 10 min). 2) Milling: sieve size: 1.0 mm, milling speed: 350 to 800 rpm. 3) Premixing II: the milled material and magnesium stearate were mixed in a hopper mixer (speed: 15 rpm, time: 10 min).
  • Delivery frequency conversion (Hz): 7 to 13; tableting frequency conversion (Hz): 20 to 40; granulation frequency conversion (Hz): 30 to 50; granulation sieve size: 1.0 mm; powder mesh number: 60 mesh.
  • the fine granules obtained by dry granulation and a prescription amount of extragranular sodium stearyl fumarate were mixed in a hopper mixer for 10 min at a speed of 15 rpm.
  • the theoretical tablet weight was calculated according to the particle content.
  • the mixture was compressed into tablets after adjusting the tablet weight and hardness.
  • a coating liquid with a solid content of 10% was formulated with a film-coating premix (gastric-soluble type) and a prescription amount of purified water. The coating liquid was stirred for 60 minutes, and filtered with a 100-mesh sieve for later use.
  • Coating the corresponding parameters were set according to the process requirements. During the coating process, the bed temperature was controlled at 30 to 40° C., and the coating weight gain was 2.0 to 4.0%.
  • a pharmaceutical composition comprising the mesylate salt of N-(5-((4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide comprises the components of: 129.8 mg of the active ingredient (calculated by C 30 H 35 N 7 O 2 .CH 3 SO 3 H), 90.0 mg of microcrystalline cellulose (KG802), 30.6 mg of anhydrous lactose (21AN), 42.0 mg of carboxymethyl starch sodium (Type A), 1.6 mg of intragranular sodium stearyl fumarate, 3.0 mg of extragranular sodium stearyl fumarate and 3.0 mg of magnesium stearate.
  • the prescription is as follows:
  • the immediate-release film-coated tablets comprising the above components were prepared by the following preparation method:
  • the raw materials were weighed according to the schedule list.
  • Premixing I the intragranular raw materials (microcrystalline cellulose (KG802), anhydrous lactose (21AN), carboxymethyl starch sodium (Type A), sodium stearyl fumarate, the active pharmaceutical ingredient) were mixed in a hopper mixer (speed: 15 rpm, time: 20 min). 2) Premixing II: after premixing I was completed, magnesium stearate (intragranular) was added to the hopper mixer for mixing (speed: 15 rpm, time: 10 min).
  • HFS speed 30 to 45 rpm
  • VFS speed 200 to 240 rpm
  • pressure wheel speed 3 to 7 rpm
  • pressure wheel pressure 20 to 25 KN
  • crushing knife speed 1000 to 1500 rpm
  • pressure wheel gap 1.0 mm
  • granulation sieve size 1.6 mm.
  • the fine granules obtained by dry granulation and a prescription amount of extragranular sodium stearyl fumarate were mixed in a hopper mixer for 10 min at a speed of 15 rpm.
  • the theoretical tablet weight was calculated according to the particle content.
  • the mixture was compressed into tablets after adjusting the tablet weight and hardness.
  • a coating liquid with a solid content of 10% was formulated with a film-coating premix (gastric-soluble type) and a prescription amount of purified water. The coating liquid was stirred for 60 minutes, and filtered with an 80-mesh sieve for later use.
  • Coating the corresponding parameters were set according to the process requirements. During the coating process, the bed temperature was controlled at 30 to 40° C., and the coating weight gain was 2.0 to 4.0%.
  • a pharmaceutical composition comprising the mesylate salt of N-(5-((4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide comprises the components of: 5.9 mg of the active ingredient (calculated by C 30 H 35 N 7 O 2 .CH 3 SO 3 H), 25.0 mg of microcrystalline cellulose, 54.7 mg of anhydrous lactose, 15.0 mg of carboxymethyl starch sodium, 0.5 mg of intragranular sodium stearyl fumarate, 0.9 mg of extragranular sodium stearyl fumarate and 0.5 mg of magnesium stearate.
  • the prescription is as follows:
  • the immediate-release film-coated tablets comprising the above components were prepared by the preparation method of Example 1.
  • a pharmaceutical composition comprising the mesylate salt of N-(5-((4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide comprises the components of: 31.8 mg of the active ingredient (calculated by C 30 H 35 N 7 O 2 .CH 3 SO 3 H), 30.0 mg of microcrystalline cellulose, 89.4 mg of anhydrous lactose, 30.0 mg of carboxymethyl starch sodium, 1.0 mg of intragranular sodium stearyl fumarate, 1.8 mg of extragranular sodium stearyl fumarate and 1.0 mg of magnesium stearate.
  • the immediate-release film-coated tablets comprising the above components were prepared by the preparation method of Example 2.
  • a pharmaceutical composition comprising the mesylate salt of N-(5-((4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide comprises the components of: 47.2 mg of the active ingredient (calculated by C 30 H 35 N 7 O 2 .CH 3 SO 3 H), 30.0 mg of microcrystalline cellulose, 93 mg of anhydrous lactose, 30.0 mg of carboxymethyl starch sodium, 1.0 mg of intragranular sodium stearyl fumarate, 1.8 mg of extragranular sodium stearyl fumarate and 2.0 mg of magnesium stearate.
  • the immediate-release film-coated tablets comprising the above components were prepared by the preparation method of Example 1.
  • a pharmaceutical composition comprising the mesylate salt of N-(5-((4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide comprises the components of 97.35 g of the active ingredient (calculated by C 30 H 35 N 7 O 2 .CH 3 SO 3 H), 67.5 g of microcrystalline cellulose, 22.95 g of anhydrous lactose, 31.5 g of carboxymethyl starch sodium, 1.2 g of intragranular sodium stearyl fumarate, 2.25 g of extragranular sodium stearyl fumarate and 2.25 g of magnesium stearate.
  • the immediate-release film-coated tablets comprising the above components were prepared by the preparation method of Example 2.
  • a pharmaceutical composition comprising the mesylate salt of N-(5-((4-(1-cyclopropyl-TH-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide comprises the components of 259.6 g of the active ingredient (calculated by C 30 H 35 N 7 O 2 .CH 3 SO 3 H), 180.0 g of microcrystalline cellulose, 61.2 g of anhydrous lactose, 84.0 g of carboxymethyl starch sodium, 3.2 g of intragranular sodium stearyl fumarate, 6.0 g of extragranular sodium stearyl fumarate and 6.0 g of magnesium stearate.
  • the immediate-release film-coated tablets comprising the above components were prepared by the preparation method of Example 1.
  • a pharmaceutical composition comprising the mesylate salt of N-(5-((4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide comprises the components of 97.35 g of the active ingredient (calculated by C 30 H 35 N 7 O 2 .CH 3 SO 3 H), 67.5 g of microcrystalline cellulose, 22.95 g of anhydrous lactose, 31.5 g of carboxymethyl starch sodium, 1.2 g of intragranular sodium stearyl fumarate, 2.25 g of extragranular sodium stearyl fumarate and 2.25 g of magnesium stearate.
  • the immediate-release film-coated tablets comprising the above components were prepared by the preparation method of Example 2.
  • a pharmaceutical composition comprising the mesylate salt of N-(5-((4-(1-cyclopropyl-TH-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide comprises the components of 47.2 mg of the active ingredient (calculated by C 30 H 35 N 7 O 2 .CH 3 SO 3 H), 30.0 mg of sorbitol, 93 mg of calcium hydrophosphate, 30.0 mg of crospovidone XL, 1.0 mg of intragranular sodium stearyl fumarate, 1.8 mg of extragranular stearic acid and 2.0 mg of talc.
  • the immediate-release film-coated tablets comprising the above components were prepared by the preparation method of Example 1.
  • a pharmaceutical composition comprising the mesylate salt of N-(5-((4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide comprises the components of: 47.2 mg of the active ingredient (calculated by C 30 H 35 N 7 O 2 .CH 3 SO 3 H), 30.0 mg of sucrose, 93 mg of calcium hydrophosphate, 30.0 mg of crospovidone XL, 1.0 mg of intragranular sodium stearyl fumarate, 1.8 mg of extragranular stearic acid and 2.0 mg of talc.
  • the immediate-release film-coated tablets comprising the above components were prepared by the preparation method of Example 1.
  • a pharmaceutical composition comprising the mesylate salt of N-(5-((4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide comprises the components of: 97.35 g of the active ingredient (calculated by C 30 H 35 N 7 O 2 .CH 3 SO 3 H), 67.5 g of microcrystalline cellulose, 22.95 g of maltose, 31.5 g of carboxymethyl starch sodium, 1.2 g of intragranular sodium stearyl fumarate, 2.25 g of extragranular sodium stearyl fumarate and 2.25 g of magnesium stearate.
  • the immediate-release film-coated tablets comprising the above components were prepared by the preparation method of Example 2.
  • a pharmaceutical composition comprising the mesylate salt of N-(5-((4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide comprises the components of: 64.9 mg of the active ingredient (calculated by C 30 H 35 N 7 O 2 .CH 3 SO 3 H), 45.0 mg of microcrystalline cellulose (KG802), 15.3 mg of corn starch, 21.0 mg of carboxymethyl starch sodium (Type A), 0.8 mg of intragranular sodium stearyl fumarate, 1.5 mg of extragranular sodium stearyl fumarate and 1.5 mg of magnesium stearate.
  • the prescription is as follows:
  • the immediate-release film-coated tablets comprising the above components were prepared by the preparation method of Example 1.
  • 0.05% phosphoric acid solution 0.5 ml of phosphoric acid was added to 1000 ml of water and mixed well.
  • acetate buffer pH 4.5
  • phosphate buffer pH 6.8
  • phosphate buffer containing 0.1% SDS pH 6.8
  • water and water containing 0.1% SDS water and water containing 0.1% SDS.
  • the tablet prepared in the present invention was stable within 24 hours in 0.1 mol/L hydrochloric acid solution, acetate buffer (pH 4.5), phosphate buffer containing 0.1% SDS (pH 6.8), water and water containing 0.1% SDS; and the tablet degraded slightly in phosphate buffer (pH 6.8).
  • impurity A is as follows:
  • the samples prepared in other Examples had similar dissolution profile to those of the samples of Examples 1 and 2.

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Abstract

A pharmaceutical composition comprising a small molecule EGFR inhibitor and a preparation method therefor, the composition comprising N-(5-((4-(1-cyclopropyl-TH-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide, an isomer, solvate, hydrate, or pharmaceutically acceptable salt thereof, or a combination thereof that acts as an active ingredient, and at least one pharmaceutically acceptable excipient.

Description

    FIELD OF THE INVENTION
  • The present invention belongs to the field of pharmaceutical formulations, and specifically relates to a pharmaceutical composition comprising a third-generation small molecule EGFR inhibitor and a method for preparing the same.
    • BACKGROUND OF THE INVENTION
  • EGFR (Epidermal Growth Factor Receptor) is a member of the erbB receptor family, which includes transmembrane protein tyrosine kinase receptors. By binding to its ligand, such as epidermal growth factor (EGF), EGFR can form a homodimer on the cell membrane or form a heterodimer with other receptors in the family, such as erbB2, erbB3, or erbB4. The formation of these dimers can cause the phosphorylation of key tyrosine residues in EGFR cells, thereby activating a number of downstream signaling pathways in cells. These intracellular signaling pathways play an important role in cell proliferation, survival and anti-apoptosis. Disorders of EGFR signal transduction pathways, including increased expression of ligands and receptors, EGFR gene amplification and mutation and the like, can promote malignant transformation of cells, and play an important role in tumor cell proliferation, invasion, metastasis and angiogenesis. Therefore, EGFR is a reasonable target for the development of anticancer drugs.
  • N-(5-((4-(1-cyclopropyl-TH-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide, which belongs to the third-generation small molecule EGFR inhibitor, has high selectivity for inhibiting the EGFR T790M mutant, while has no or low activity on wild-type EGFR, therefore, it can be used in treating drug-resistant tumors caused by the secondary mutation of EGFR-T790M. This compound (hereinafter referred to as the compound of formula I) was first disclosed in the international patent application WO2016054987, and its structure is shown in the following formula I
  • Figure US20210069190A1-20210311-C00001
    • SUMMARY OF THE INVENTION
  • The present invention provides a pharmaceutical composition comprising a third-generation small molecule EGFR inhibitor.
  • The pharmaceutical composition comprises a mesylate salt of N-(5-((4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide, a polymorph, solvate, hydrate, pharmaceutically acceptable salt thereof or a combination thereof as the active ingredient, and at least one pharmaceutically acceptable excipient.
  • In the pharmaceutical composition according to the present invention, the active ingredient is present in an amount of 1 to 60%.
  • In the pharmaceutical composition according to the present invention, the unit dose of the active ingredient is 10 to 200 mg, preferably 55 to 110 mg, and more preferably 55 mg or 110 mg.
  • In the pharmaceutical composition according to the present invention, the excipient comprises one or more filler(s), comprising at least one disaccharide or polysaccharide, such as glucan, starch, cellulose, lactose, maltose or sucrose. The starch can be selected from the group consisting of potato starch, corn starch, rice starch, wheat starch, amylopectin, pregelatinized starch and the like. The disaccharide or polysaccharide is present in an amount of 1 to 60%, preferably 5 to 55%, more preferably 5 to 30%, and further preferably 5 to 15%.
  • The filler is preferably a disaccharide, such as lactose, and it is present in an amount of 1 to 60%, preferably 5 to 55%, more preferably 5 to 30%, and further preferably 5 to 15%.
  • In the pharmaceutical composition according to the present invention, the excipient comprises one or more filler(s), disintegrant(s) or lubricant(s).
  • In the pharmaceutical composition according to the present invention, the weight percentage of each component is as follows:
  •
    active ingredient  1 to 60%
    filler 20 to 80%
    disintegrant  1 to 30%
    lubricant 0.1 to 10%.
  • Preferably, the weight percentage of each component is as follows:
  •
    active ingredient 35 to 50%
    filler 30 to 50%
    disintegrant 10 to 20%
    lubricant  0.5 to 5%.
  • More preferably, the weight percentage of each component is as follows:
  •
    active ingredient 43.3%
    filler 40.2%
    disintegrant  14%
    lubricant  2.5%.
  • In the pharmaceutical composition according to the present invention, the filler comprises one or more of microcrystalline cellulose, lactose, anhydrous lactose, corn starch, pregelatinized starch, mannitol, sorbitol, calcium hydrophosphate and calcium sulfate, wherein the filler is present in an amount of 20 to 80%, and preferably 30 to 50%. Preferably, the filler comprises two of the above components, and the weight ratio of the two components is 1:3 to 3:1.
  • In the pharmaceutical composition according to the present invention, the filler is preferably microcrystalline cellulose and lactose. The microcrystalline cellulose is present in an amount of 1 to 60%, preferably 10 to 40%, and more preferably 20 to 40%, relative to the weight of the tablet core; and the lactose is present in an amount of 1 to 60%, preferably 5 to 30%, and more preferably 5 to 15%, relative to the weight of the tablet core. Optional technical solutions include, but are not limited to, the microcrystalline cellulose is present in an amount of 1 to 60%, and the lactose is present in an amount of 1 to 60%; the microcrystalline cellulose is present in an amount of 10 to 40%, and the lactose is present in an amount of 5 to 30%; and the microcrystalline cellulose is present in an amount of 20 to 40%, and the lactose is present in an amount of 5 to 15%.
  • Further preferably, the weight ratio of microcrystalline cellulose to lactose is 1:3 to 3:1, and more preferably 2-3:1.
  • Further preferably, the lactose is anhydrous lactose. The microcrystalline cellulose is present in an amount of 1 to 60%, preferably 10 to 40%, and more preferably 20 to 40%, relative to the weight of the tablet core; and the anhydrous lactose is present in an amount of 1 to 60%, preferably 5 to 30%, and more preferably 5 to 15%, relative to the weight of the tablet core. Optional technical solutions include, but are not limited to, the microcrystalline cellulose is present in an amount of 1 to 60%, and the anhydrous lactose is present in an amount of 1 to 60%; the microcrystalline cellulose is present in an amount of 10 to 40%, and the anhydrous lactose is present in an amount of 5 to 30%; and the microcrystalline cellulose is present in an amount of 20 to 40%, and the anhydrous lactose is present in an amount of 5 to 15%.
  • Further preferably, the weight ratio of microcrystalline cellulose to anhydrous lactose is 1:3 to 3:1, more preferably 2-3:1.
  • Through the addition of lactose, the pharmaceutical composition with a high content of the compound of formula I can be prepared, and its drug loading capacity can reach more than 40%. At the same time, the resulting pharmaceutical composition has a good compressibility and uniform dissolution.
  • In the pharmaceutical composition according to the present invention, the disintegrant comprises one or more of low-substituted hydroxypropyl cellulose, croscarmellose sodium, carboxymethyl starch sodium and crospovidone, and preferably carboxymethyl starch sodium. The disintegrant is present in an amount of 1 to 30%, and preferably 10 to 20%. The disintegrant that is added in a totally intragranular manner can ensure good disintegration and preparation of the tablet. The addition of carboxymethyl starch sodium can further improve the stability of the formulation. Preferably, the disintegrant is added intragranularly.
  • In the pharmaceutical composition according to the present invention, the lubricant comprises one or more of talc, stearic acid, sodium stearyl fumarate, glyceryl behenate, magnesium stearate and micronized silica gel. The lubricant is present in an amount of 0.1 to 10%, and preferably 0.2 to 5%, relative to the weight of the tablet core.
  • Further, the lubricant is preferably sodium stearyl fumarate and magnesium stearate. The sodium stearyl fumarate is present in an amount of 0.1 to 5%, and preferably 0.3 to 3%, and the magnesium stearate is present in an amount of 0.1 to 5%, and preferably 0.2 to 2%. The combination of sodium stearyl fumarate and magnesium stearate can obviously improve the adhesion of raw materials on the surface of the equipment, ensure a smooth preparation process, and enable the finished product to have an excellent dissolution effect.
  • In the pharmaceutical composition according to the present invention, the amount refers to the percentage relative to the total weight of the drug, wherein the total weight of the drug does not include the weight of the coating. As for specific dosage forms, the amount refers to the percentage relative to the weight of the tablet core or granule.
  • The pharmaceutical composition according to the present invention comprises the following components:
  •
    active ingredient 35 to 50%
    lactose  5 to 15%
    microcrystalline cellulose 30 to 50%
    carboxymethyl starch sodium 10 to 20%
    lubricant  0.5 to 5%.
  • Preferably, it comprises the following components:
  •
    active ingredient 43.3%
    lactose 10.2%
    microcrystalline cellulose 30.0%
    carboxymethyl starch sodium 10.4%
    lubricant 0.5 to 5%. 
  • More preferably, it comprises the following components:
  •
    active ingredient 43.3%
    lactose 10.2%
    microcrystalline cellulose 30.0%
    carboxymethyl starch sodium  14%
    lubricant  2.5%.
  • Most preferably, it comprises the following components:
  •
    active ingredient 43.3%
    lactose 10.2%
    microcrystalline cellulose 30.0%
    carboxymethyl starch sodium  14%
    magnesium stearate   1%
    sodium stearyl fumarate  1.5%.
  • The pharmaceutical composition of the present invention is an oral formulation selected from the group consisting of tablet and capsule, preferably a film, and more preferably an immediate-release film-coated tablet. The coating agent is a gastric-soluble film coating premix, preferably Opadry 85F140124 (the main ingredients are polyvinyl alcohol, polyethylene glycol, talc, titanium dioxide, iron oxide red and iron oxide black), and Opadry 85F12300 (the main ingredients are polyvinyl alcohol, polyethylene glycol, talc, titanium dioxide and iron oxide yellow).
  • The present invention further provides a method for preparing the pharmaceutical composition, mainly comprising the following steps of:
  • 1) pre-treatment of raw materials: sieving the filler, disintegrant and lubricant for later use;
  • 2) mixing: weighing the intragranular raw materials according to specified amounts and mixing them;
  • 3) dry granulation: granulating the above mixed powder by dry granulation;
  • 4) total mixing: mixing the resulting granules and extragranular lubricant;
  • 5) optionally, tableting: compressing the resulting mixture into tablets according to the theoretical tablet weight; and
  • 6) optionally, coating.
  • The above method comprises the specific steps of:
  • 1) pre-treatment of raw materials: sieving microcrystalline cellulose and anhydrous lactose with a 60-mesh sieve, and sieving sodium stearyl fumarate and carboxymethyl starch sodium with an 80-mesh sieve for later use;
  • 2) mixing: weighing the intragranular raw materials according to prescription amounts, and mixing microcrystalline cellulose, anhydrous lactose, carboxymethyl starch sodium, sodium stearyl fumarate, the active ingredient and magnesium stearate with a hopper mixer;
  • 3) dry granulation: granulating the above mixed powder with a dry granulator;
  • 4) total mixing: mixing the resulting fine granules and prescription amount of extragranular sodium stearyl fumarate with a hopper mixer;
  • 5) optionally, tableting: compressing the resulting mixture into tablets according to the theoretical tablet weight; and
  • 6) optionally, coating: i) formulation of a coating liquid, adding a prescription amount of Opadry to purified water under stirring to formulate a coating liquid with a solid content of 10%, stirring the coating liquid for 60 minutes after the completion of the addition of Opadry to make it evenly dispersed, and sieving the formulated coating liquid with a 100-mesh sieve for later use; and ii) setting the parameters according to the process requirements, and finishing the coating until the coating weight gain reaches about 2.0% to 4.0%.
  • The inventor finds through a large number of studies that N-(5-((4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide, as third-generation small molecule EGFR inhibitor, is a drug with low permeability. Its solubility is greatly affected by pH, which is pH dependent. The pharmaceutical composition prepared according to the present invention has a good and uniform dissolution in different dissolution media.
    • DETAILED DESCRIPTION OF THE INVENTION
  • Regarding to the Filler:
  • It can be found from Table 1 that when the filler comprises lactose, a pharmaceutical composition with a high content of the compound of formula I can be prepared, and the resulting pharmaceutical composition has a good compressibility.
  • TABLE 1
    Screening of the filler
    Single-factor study Sample 1 Sample 2 Sample 3
    Active ingredient 1  1.416  1.416 1.416
    Microcrystalline cellulose 1.05 1.05 1.05
    Anhydrous lactose 3.15 / 1.56
    Mannitol / 3.15 1.59
    Croscarmellose sodium 0.18 0.18 0.18
    (added intragranularly)
    Croscarmellose sodium 0.09 0.09 0.09
    (added extragranularly)
    Magnesium stearate 0.09 0.09 0.09
    (added intragranularly)
    Magnesium stearate  0.084  0.084 0.084
    (added extragranularly)
    Process of compressing There was no There was punch There was punch
    large tablets (called punch sticking, sticking, and the sticking, and the
    “slugs”) and the large large tablets were large tablets were
    tablets were not smooth and not smooth and
    smooth and does not meet the does not meet the
    100% qualified production production
    requirement requirement
    Note:
    1 The active ingredient is calculated by mesylate salt, the same below.
  • The drug loading capacity of the active ingredient in the tablet was further increased. When the active ingredient was present in an amount of 43.3%, the tablet still had a very good compressibility. There was no punch sticking. The resulting uncoated tablets were smooth and 100% qualified, having a complete shape and good appearance.
  • TABLE 2
    Type and dosage of the filler
    Microcrystalline cellulose (PH102) + anhydrous
    lactose (21AN)
    Single-factor study Ratio: 3:1 Ratio: 1:1 Ratio: 1:3
    Batch 200 Tablets/g 200 Tablets/g 200 Tablets/g
    Active ingredient1 25.96 25.96 25.96
    Microcrystalline cellulose 18.00 12.00 6.00
    (PH102)
    Anhydrous lactose (21AN) 6.14 12.14 18.14
    Carboxymethyl starch 8.40 8.40 8.40
    sodium (Type A)
    Sodium stearyl fumarate 0.3 0.3 0.3
    (added intragranularly)
    Magnesium stearate 0.6 0.6 0.6
    (added intragranularly)
    Sodium stearyl fumarate 0.6 0.6 0.6
    (added extragranularly)
    Process of compressing There was no There was no There was no
    large tablets (called punch sticking, punch sticking, punch sticking,
    “slugs”) and the large and the large and the large
    tablets were tablets were tablets were
    smooth smooth smooth
    Hardness of uncoated 6.60 6.67 6.89
    tablet (kgf)
    Cumulative 0.1M 15 90 83 89
    dissolution HCl min
    rate (%)
  • Moreover, the hardness of uncoated tablet is also one of the factors that affect the dissolution rate. Uneven hardness may lead to uneven dissolution, thereby affecting the efficacy. Table 3 below shows the corresponding dissolution profile of the prescription of the present invention at different hardnesses. It can be seen from the table that the prescription of the present invention can eliminate the effect of hardness changes on the dissolution result of the uncoated tablet under different hardness conditions, and enable the product to maintain a good dissolution rate. Therefore, the prescription of the present invention can overcome the adverse effect caused by the different hardness of uncoated tablets.
  • TABLE 3
    Dissolution rate test of the prescription of the present invention at different hardnesses
    Proportion (%) Prescription amount (g)
    Active ingredient 43.27 25.96
    Microcrystalline cellulose 30.00 18.0
    Anhydrous lactose 10.23 6.14
    Carboxymethyl starch 14.00 8.4
    sodium
    Sodium stearyl fumarate 0.50 0.3
    (added intragranularly)
    Magnesium stearate (added 1.00 0.6
    intragranularly)
    Sodium stearyl fumarate 1.00 0.6
    (added extragranularly)
    Hardness range (kgf) 5.50 10.30 6.20
    Dissolution profile in 30 Almost no Almost no Almost no
    minutes accumulation at the accumulation at the accumulation at the
    bottom of the bottle bottom of the bottle bottom of the bottle
    Cumulative 0.1M n = 6 Averange RSD Averange RSD Averange RSD
    dissolution hydrochloric  5 min 41 13.1 36 5.9 60 5.3
    rate (%) acid 15 min 88 2.7 88 1.4 91 3.4
    30 min 90 1.9 92 1.2 93 2.3
  • Regarding to the Disintegrant:
  • In the pharmaceutical composition system of the present invention, the addition of the disintegrant can also affect the dissolution rate of the tablet. The inventor finds that the dissolution rate is good when the disintegrant is present in an amount of 1 to 30%. See Table 4 for details. Carboxymethyl starch sodium used as the disintegrant can ensure a good disintegration and preparation of the tablet, and the preferred dosage is 10% to 20%.
  • TABLE 4
    Dosage and adding method of the disintegrant
    Dosage and adding method of the disintegrant
    Carboxymethyl Carboxymethyl
    Carboxymethyl Carboxymethyl Carboxymethyl Carboxymethyl starch sodium starch sodium
    starch sodium starch sodium starch sodium starch sodium (16%) added (14%) added
    (14%) added (6%) added (16%) added (8%) added intragranularly:added intragranularly:added
    Single-factor study intragranularly intragranularly intragranularly intragranularly extragranularly = 1:1 extragranularly = 1:1
    Batch 200 Tablets/g 200 Tablets/g 200 Tablets/g 200 Tablets/g 200 Tablets/g 200 Tablets/g
    Active ingredient 25.96 25.96 25.96 25.96 25.96 25.96
    Microcrystalline 18.0 21.6 17.1 19.8 17.1 18.0
    cellulose (PH102)
    Anhydrous lactose 6.14 7.34 5.84 6.74 5.84 6.14
    (21AN)
    Carboxymethyl starch 8.4 3.6 9.6 6.0 4.8 4.2
    sodium, Type A (added
    intragranularly)
    Sodium stearyl 0.3 0.3 0.3 0.3 0.3 0.3
    fumarate (added
    intragranularly)
    Magnesium stearate 0.6 0.6 0.6 0.6 0.6 0.6
    (added intragranularly)
    Carboxymethyl starch / / / / 4.8 4.2
    sodium, Type A (added
    extragranularly)
    Sodium stearyl 0.6 0.6 0.6 0.6 0.6 0.6
    fumarate (added
    extragranularly)
    Hardness of uncoated 6.60 6.53 6.53 6.53 6.44 6.37
    tablet (kgf)
    Cumulative 0.1M 15 min 90 74 88 72 86 82
    dissolution HCl
    rate (%)
    • Example 1
  • A pharmaceutical composition comprising the mesylate salt of N-(5+(4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide comprises the components of: 64.9 mg of the active ingredient (calculated by C30H35N7O2.CH3SO3H), 45.0 mg of microcrystalline cellulose (KG802), 15.3 mg of anhydrous lactose (21AN), 21.0 mg of carboxymethyl starch sodium (Type A), 0.8 mg of intragranular sodium stearyl fumarate, 1.5 mg of extragranular sodium stearyl fumarate and 1.5 mg of magnesium stearate. The prescription is as follows:
  •
    Mesylate salt of the compound of formula I 43.3%
    Microcrystalline cellulose 30.0%
    Anhydrous lactose 10.2%
    Carboxymethyl starch sodium  14%
    Sodium stearyl fumarate 0.5% + 1.0%
    Magnesium stearate  1.0%
  • The immediate-release film-coated tablets comprising the above components were prepared by the following preparation method:
  • (1) Pre-Treatment of Raw Materials
  • Material name Treatment method
    Microcrystalline cellulose (KG802) Sieved with a 60-mesh sieve
    Anhydrous lactose (21AN) Sieved with a 60-mesh sieve
    Carboxymethyl starch sodium (Type A) Sieved with a 80-mesh sieve
    Sodium stearyl fumarate Sieved with a 80-mesh sieve
  • (2) Weighing
  • The raw materials were weighed according to the schedule list.
  • (3) Mixing
  • 1) Premixing I: the intragranular raw materials were mixed in a hopper mixer (speed: 15 rpm, time: 10 min). 2) Milling: sieve size: 1.0 mm, milling speed: 350 to 800 rpm. 3) Premixing II: the milled material and magnesium stearate were mixed in a hopper mixer (speed: 15 rpm, time: 10 min).
  • (4) Dry Granulation
  • Delivery frequency conversion (Hz): 7 to 13; tableting frequency conversion (Hz): 20 to 40; granulation frequency conversion (Hz): 30 to 50; granulation sieve size: 1.0 mm; powder mesh number: 60 mesh.
  • (5) Total Mixing
  • The fine granules obtained by dry granulation and a prescription amount of extragranular sodium stearyl fumarate were mixed in a hopper mixer for 10 min at a speed of 15 rpm.
  • (6) Tableting
  • The theoretical tablet weight was calculated according to the particle content. The mixture was compressed into tablets after adjusting the tablet weight and hardness.
  • (7) Coating
  • 1) Formulation of a coating liquid: a coating liquid with a solid content of 10% was formulated with a film-coating premix (gastric-soluble type) and a prescription amount of purified water. The coating liquid was stirred for 60 minutes, and filtered with a 100-mesh sieve for later use.
  • 2) Coating: the corresponding parameters were set according to the process requirements. During the coating process, the bed temperature was controlled at 30 to 40° C., and the coating weight gain was 2.0 to 4.0%.
  • (8) Packaging.
    • Example 2
  • A pharmaceutical composition comprising the mesylate salt of N-(5-((4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide comprises the components of: 129.8 mg of the active ingredient (calculated by C30H35N7O2.CH3SO3H), 90.0 mg of microcrystalline cellulose (KG802), 30.6 mg of anhydrous lactose (21AN), 42.0 mg of carboxymethyl starch sodium (Type A), 1.6 mg of intragranular sodium stearyl fumarate, 3.0 mg of extragranular sodium stearyl fumarate and 3.0 mg of magnesium stearate. The prescription is as follows:
  •
    Mesylate salt of the compound of formula I 43.3%
    Microcrystalline cellulose 30.0%
    Anhydrous lactose 10.2%
    Carboxymethyl starch sodium  14%
    Sodium stearyl fumarate 0.5% + 1.0%
    Magnesium stearate  1.0%
  • The immediate-release film-coated tablets comprising the above components were prepared by the following preparation method:
  • (1) Pre-Treatment of Raw Materials
  • Material name Treatment method
    Microcrystalline cellulose (KG802) Sieved with a 60-mesh sieve
    Anhydrous lactose (21AN) Sieved with a 60-mesh sieve
    Carboxymethyl starch sodium (Type A) Sieved with a 80-mesh sieve
    Sodium stearyl fumarate Sieved with a 80-mesh sieve
  • (2) Weighing
  • The raw materials were weighed according to the schedule list.
  • (3) Mixing
  • 1) Premixing I: the intragranular raw materials (microcrystalline cellulose (KG802), anhydrous lactose (21AN), carboxymethyl starch sodium (Type A), sodium stearyl fumarate, the active pharmaceutical ingredient) were mixed in a hopper mixer (speed: 15 rpm, time: 20 min). 2) Premixing II: after premixing I was completed, magnesium stearate (intragranular) was added to the hopper mixer for mixing (speed: 15 rpm, time: 10 min).
  • (4) Dry Granulation
  • HFS speed: 30 to 45 rpm, VFS speed: 200 to 240 rpm, pressure wheel speed: 3 to 7 rpm, pressure wheel pressure: 20 to 25 KN, crushing knife speed: 1000 to 1500 rpm, pressure wheel gap: 1.0 mm, granulation sieve size: 1.6 mm.
  • (5) Total Mixing
  • The fine granules obtained by dry granulation and a prescription amount of extragranular sodium stearyl fumarate were mixed in a hopper mixer for 10 min at a speed of 15 rpm.
  • (6) Tableting
  • The theoretical tablet weight was calculated according to the particle content. The mixture was compressed into tablets after adjusting the tablet weight and hardness.
  • (7) Coating
  • 1) Formulation of a coating liquid: a coating liquid with a solid content of 10% was formulated with a film-coating premix (gastric-soluble type) and a prescription amount of purified water. The coating liquid was stirred for 60 minutes, and filtered with an 80-mesh sieve for later use.
  • 2) Coating: the corresponding parameters were set according to the process requirements. During the coating process, the bed temperature was controlled at 30 to 40° C., and the coating weight gain was 2.0 to 4.0%.
  • (8) Packaging.
    • Example 3
  • A pharmaceutical composition comprising the mesylate salt of N-(5-((4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide comprises the components of: 5.9 mg of the active ingredient (calculated by C30H35N7O2.CH3SO3H), 25.0 mg of microcrystalline cellulose, 54.7 mg of anhydrous lactose, 15.0 mg of carboxymethyl starch sodium, 0.5 mg of intragranular sodium stearyl fumarate, 0.9 mg of extragranular sodium stearyl fumarate and 0.5 mg of magnesium stearate. The prescription is as follows:
  •
    Mesylate salt of the compound of formula I 5.8%
    Microcrystalline cellulose 24.4%
    Anhydrous lactose 53.4%
    Carboxymethyl starch sodium 14.6%
    Sodium stearyl fumarate 0.5% + 0.8%
    Magnesium stearate 0.5%
  • The immediate-release film-coated tablets comprising the above components were prepared by the preparation method of Example 1.
    • Example 4
  • A pharmaceutical composition comprising the mesylate salt of N-(5-((4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide comprises the components of: 31.8 mg of the active ingredient (calculated by C30H35N7O2.CH3SO3H), 30.0 mg of microcrystalline cellulose, 89.4 mg of anhydrous lactose, 30.0 mg of carboxymethyl starch sodium, 1.0 mg of intragranular sodium stearyl fumarate, 1.8 mg of extragranular sodium stearyl fumarate and 1.0 mg of magnesium stearate.
  • The immediate-release film-coated tablets comprising the above components were prepared by the preparation method of Example 2.
  •
    Mesylate salt of the compound of formula I 17.2%
    Microcrystalline cellulose 16.2%
    Anhydrous lactose 48.3%
    Carboxymethyl starch sodium 16.2%
    Sodium stearyl fumarate 0.5% + 1.0%
    Magnesium stearate  0.5%
    • Example 5
  • A pharmaceutical composition comprising the mesylate salt of N-(5-((4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide comprises the components of: 47.2 mg of the active ingredient (calculated by C30H35N7O2.CH3SO3H), 30.0 mg of microcrystalline cellulose, 93 mg of anhydrous lactose, 30.0 mg of carboxymethyl starch sodium, 1.0 mg of intragranular sodium stearyl fumarate, 1.8 mg of extragranular sodium stearyl fumarate and 2.0 mg of magnesium stearate.
  •
    Mesylate salt of the compound of formula I 23.0%
    Microcrystalline cellulose 14.6%
    Anhydrous lactose 45.4%
    Carboxymethyl starch sodium 14.6%
    Sodium stearyl fumarate 0.5% + 0.9%
    Magnesium stearate  1.0%
  • The immediate-release film-coated tablets comprising the above components were prepared by the preparation method of Example 1.
    • Example 6
  • A pharmaceutical composition comprising the mesylate salt of N-(5-((4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide comprises the components of 97.35 g of the active ingredient (calculated by C30H35N7O2.CH3SO3H), 67.5 g of microcrystalline cellulose, 22.95 g of anhydrous lactose, 31.5 g of carboxymethyl starch sodium, 1.2 g of intragranular sodium stearyl fumarate, 2.25 g of extragranular sodium stearyl fumarate and 2.25 g of magnesium stearate.
  •
    Mesylate salt of the compound of formula I 43.3%
    Microcrystalline cellulose 30.0%
    Anhydrous lactose 10.2%
    Carboxymethyl starch sodium 14.0%
    Sodium stearyl fumarate 0.5% + 1.0%
    Magnesium stearate  1.0%
  • The immediate-release film-coated tablets comprising the above components were prepared by the preparation method of Example 2.
    • Example 7
  • A pharmaceutical composition comprising the mesylate salt of N-(5-((4-(1-cyclopropyl-TH-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide comprises the components of 259.6 g of the active ingredient (calculated by C30H35N7O2.CH3SO3H), 180.0 g of microcrystalline cellulose, 61.2 g of anhydrous lactose, 84.0 g of carboxymethyl starch sodium, 3.2 g of intragranular sodium stearyl fumarate, 6.0 g of extragranular sodium stearyl fumarate and 6.0 g of magnesium stearate.
  •
    Mesylate salt of the compound of formula I 43.3%
    Microcrystalline cellulose 30.0%
    Anhydrous lactose 10.2%
    Carboxymethyl starch sodium 14.0%
    Sodium stearyl fumarate 0.5% + 1.0%
    Magnesium stearate  1.0%
  • The immediate-release film-coated tablets comprising the above components were prepared by the preparation method of Example 1.
    • Example 8
  • A pharmaceutical composition comprising the mesylate salt of N-(5-((4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide comprises the components of 97.35 g of the active ingredient (calculated by C30H35N7O2.CH3SO3H), 67.5 g of microcrystalline cellulose, 22.95 g of anhydrous lactose, 31.5 g of carboxymethyl starch sodium, 1.2 g of intragranular sodium stearyl fumarate, 2.25 g of extragranular sodium stearyl fumarate and 2.25 g of magnesium stearate.
  •
    Mesylate salt of the compound of formula I 43.3%
    Microcrystalline cellulose 30.0%
    Anhydrous lactose 10.2%
    Carboxymethyl starch sodium 14.0%
    Sodium stearyl fumarate 0.5% + 1.0%
    Magnesium stearate  1.0%
  • The immediate-release film-coated tablets comprising the above components were prepared by the preparation method of Example 2.
    • Example 9
  • A pharmaceutical composition comprising the mesylate salt of N-(5-((4-(1-cyclopropyl-TH-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide comprises the components of 47.2 mg of the active ingredient (calculated by C30H35N7O2.CH3SO3H), 30.0 mg of sorbitol, 93 mg of calcium hydrophosphate, 30.0 mg of crospovidone XL, 1.0 mg of intragranular sodium stearyl fumarate, 1.8 mg of extragranular stearic acid and 2.0 mg of talc.
  •
    Mesylate salt of the compound of formula I 23.0%
    Sorbitol 14.6%
    Calcium hydrophosphate 45.4%
    Crospovidone 14.6%
    Sodium stearyl fumarate 0.5%
    Stearic acid 0.9%
    Talc 1.0%
  • The immediate-release film-coated tablets comprising the above components were prepared by the preparation method of Example 1.
    • Example 10
  • A pharmaceutical composition comprising the mesylate salt of N-(5-((4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide comprises the components of: 47.2 mg of the active ingredient (calculated by C30H35N7O2.CH3SO3H), 30.0 mg of sucrose, 93 mg of calcium hydrophosphate, 30.0 mg of crospovidone XL, 1.0 mg of intragranular sodium stearyl fumarate, 1.8 mg of extragranular stearic acid and 2.0 mg of talc.
  •
    Mesylate salt of the compound of formula I 23.0%
    Sucrose 14.6%
    Calcium hydrophosphate 45.4%
    Crospovidone 14.6%
    Sodium stearyl fumarate 0.5%
    Stearic acid 0.9%
    Talc 1.0%
  • The immediate-release film-coated tablets comprising the above components were prepared by the preparation method of Example 1.
    • Example 11
  • A pharmaceutical composition comprising the mesylate salt of N-(5-((4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide comprises the components of: 97.35 g of the active ingredient (calculated by C30H35N7O2.CH3SO3H), 67.5 g of microcrystalline cellulose, 22.95 g of maltose, 31.5 g of carboxymethyl starch sodium, 1.2 g of intragranular sodium stearyl fumarate, 2.25 g of extragranular sodium stearyl fumarate and 2.25 g of magnesium stearate.
  •
    Mesylate salt of the compound of formula I 43.3%
    Microcrystalline cellulose 30.0%
    Maltose 10.2%
    Carboxymethyl starch sodium 14.0%
    Sodium stearyl fumarate 0.5% + 1.0%
    Magnesium stearate  1.0%
  • The immediate-release film-coated tablets comprising the above components were prepared by the preparation method of Example 2.
    • Example 12
  • A pharmaceutical composition comprising the mesylate salt of N-(5-((4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide comprises the components of: 64.9 mg of the active ingredient (calculated by C30H35N7O2.CH3SO3H), 45.0 mg of microcrystalline cellulose (KG802), 15.3 mg of corn starch, 21.0 mg of carboxymethyl starch sodium (Type A), 0.8 mg of intragranular sodium stearyl fumarate, 1.5 mg of extragranular sodium stearyl fumarate and 1.5 mg of magnesium stearate. The prescription is as follows:
  •
    Mesylate salt of the compound of formula I 43.3%
    Microcrystalline cellulose 30.0%
    Corn starch 10.2%
    Carboxymethyl starch sodium  14%
    Sodium stearyl fumarate 0.5% + 1.0%
    Magnesium stearate  1.0%
  • The immediate-release film-coated tablets comprising the above components were prepared by the preparation method of Example 1.
  • Stability Test and Result Analysis
  • 1. Stability of the Product in Different Dissolution Media
  • Chromatography Condition:
  • Instruments and reagents High performance liquid chromatograph, electronic analytical balance, potassium dihydrogen phosphate (chromatographically pure), acetonitrile (chromatographically pure), reference substance for system suitability of related substance
  • Chromatography CONDITION Octadecylsilane bonded silica gel as the filler (Waters XBridge C18, 4.6 mm×150 mm, 3.5 μm or a column with equivalent performance); flow rate: 1.0 ml/min; detection wavelength: 220 nm; column temperature: 35° C.; injection volume: 10 μl.
  • Mobile phase A 2.72 g of potassium dihydrogen phosphate was dissolved in about 900 ml of water, the pH was adjusted to 6.0 with sodium hydroxide solution, water was added until the volume reached 1000 ml, and then the solution was mixed well, filtrated and degased.
  • Mobile phase B Acetonitrile
  • Gradient elution was carried out according to the following table (the amount of mobile phase is scalable)
  • Time (min) 0 20 30 40 42 50
    Mobile phase A (%) 80 60 30 30 80 80
    Mobile phase B (%) 20 40 70 70 20 20
  • 0.05% phosphoric acid solution 0.5 ml of phosphoric acid was added to 1000 ml of water and mixed well.
  • Diluent 800 ml of 0.05% phosphoric acid solution and 200 ml of acetonitrile were mixed well.
  • TABLE 5
    Results of the stability study of the product in different media
    Dissolution Time (hour)
    medium 0 hour 1 hour 2 hours 4 hours 8 hours 24 hours
    0.1 mol/L Peak area 5817.502 5724.833 5715.132 5709.908 5691.358 5709.127
    hydrochloric Relative 100.0% 98.4% 98.2% 98.2% 97.8% 98.1%
    acid solution content
    Acetate Peak area 5388.433 5313.248 5301.212 5294.096 5282.456 5308.160
    buffer, pH 4.5 Relative 100.0% 98.6% 98.4% 98.2% 98.0% 98.5%
    content
    Phosphate Peak area 3364.067 3310.739 3294.386 3270.577 3264.820 3236.565
    buffer, pH 6.8 Relative 100.0% 98.4% 97.9% 97.2% 97.0% 96.2%
    content
    Phosphate Peak area 4868.623 4864.179 4861.332 4865.548 4853.247 4837.464
    buffer, pH 6.8 Relative 100.0% 99.9% 99.9% 99.9% 99.7% 99.4%
    (containing content
    0.1% SDS)
    Water Peak area 3627.570 3623.876 3610.467 3602.525 3592.348 3590.479
    Relative 100.0% 99.9% 99.5% 99.3% 99.0% 99.0%
    content
    Water Peak area 5456.676 5443.102 5433.346 5430.342 5421.376 5422.712
    (containing Relative 100.0% 99.8% 99.6% 99.5% 99.4% 99.4%
    0.1% SDS) content
  • The inventor studied the solution stability of the tablet prepared in Example 1 as example in 0.1 mol/L hydrochloric acid solution, acetate buffer (pH 4.5), phosphate buffer (pH 6.8), phosphate buffer containing 0.1% SDS (pH 6.8), water and water containing 0.1% SDS. According to the dissolution and release rate test (the second method described in general rule 0931 of volume IV of the Chinese Pharmacopoeia 2015 Edition) under a condition of 50 rpm, an eluate was taken at 30 minutes, and then filtered. The filtrate was used as the test solution to study the solution stability at room temperature within 24 hours. The solution stability data is shown in Table 4.
  • The results showed that the tablet prepared in the present invention was stable within 24 hours in 0.1 mol/L hydrochloric acid solution, acetate buffer (pH 4.5), phosphate buffer containing 0.1% SDS (pH 6.8), water and water containing 0.1% SDS; and the tablet degraded slightly in phosphate buffer (pH 6.8).
  • 2. Stability Under Light, High Temperature or High Humidity Conditions
  • TABLE 6
    Results of influencing factor study (study condition: high temperature)
    Storage condition
    40° C. 60° C.
    Amount for quality study at each storage point
    63 25 25 25 63 25 25 25
    tablets tablets tablets tablets tablets tablets tablets tablets
    Standard Storage time
    Study item requirement 0 d 5d 10 d 30 d 0 d 5d 10 d 30 d
    Liquid chromatography The retention time Same Same Same Same Same Same Same Same
    identification should be the same
    Dissolution rate (%) Should be ≥80 98 98 94 95 98 99 99 97
    15 min
    Related Impurity A2 Should be ≤2.0 0.10 0.14 0.11 0.21 0.10 0.11 0.07 0.10
    substance Other Should be ≤0.2 0.04 0.04 0.05 0.01 0.04 0.02 0.03 0.04
    (%) maximum
    single
    impurity
    Total Should be ≤3.0 0.15 0.19 0.17 0.24 0.15 0.16 0.14 0.19
    impurity
    Moisture (%) Should be ≤3.0 2.3 2.4 2.4 1.6 2.3 2.5 2.4 1.6
    Crystal form Should be the same Meet the / Meet the Meet the Meet the / Meet the Meet the
    as that at Day 0 require- require- require- require- require- require-
    ment ment ment ment ment ment
    Content (%) Should be 90.0 to 99.6 101.1 98.8 99.3 99.6 100.0 99.5 99.1
    110.0
    Note:
    2the RRT of impurity A is about 1.23.
  • The structure of impurity A is as follows:
  • Figure US20210069190A1-20210311-C00002
  • TABLE 7
    Results of influencing factor study (study condition: high humidity)
    Storage condition
    25° C./RH75% (saturated sodium chloride solution)
    Amount for quality study at each storage point
    63 tablets 25 tablets 25 tablets 25 tablets
    Storage time
    Study item Standard requirement 0 d 5 d 10 d 30 d
    Liquid chromatography The retention time Same Same Same Same
    identification should be the same
    Dissolution rate (%) Should be ≥80 98 98 97 95
    Related Impurity A Should be ≤2.0 0.10 0.16 0.09 0.21
    substance Other Should be ≤0.2 0.04 0.04 0.04 0.01
    (%) maximum
    single
    impurity
    Total Should be ≤3.0 0.15 0.19 0.13 0.25
    impurity
    Moisture (%) Should be ≤3.0 2.3 3.7 3.5 3.9
    Crystal form Should be the same Meet the / Meet the Meet the
    as that at Day 0 requirement requirement requirement
    Content (%) Should be 90.0 to 99.6 101.3 99.6 99.6
    110.0
  • TABLE 8
    Results of influencing factor study (study condition: light)
    Storage condition
    Light
    Amount for quality test
    at each storage point
    310 tablets 25 tablets 25 tablets
    Storage time
    Study item Standard requirement 0 d 5 d 10 d
    Liquid chromatography The retention time Same Same Same
    identification should be the same
    Dissolution rate (%) Should be ≥80 98 98 97
    Related Impurity A Should be ≤2.0 0.10 0.13 0.10
    substance Other maximum Should be ≤0.2 0.04 0.04 0.04
    (%) single impurity
    Total impurity Should be ≤3.0 0.15 0.17 0.16
    Moisture (%) Should be ≤3.0 2.3 2.7 2.4
    Crystal form Should be the same as Meet the / Meet the
    that at Day 0 requirement requirement
    Content (%) Should be 90.0 to 110.0 99.6 101.2 98.7
  • The results are shown in Tables 6 to 8. The influencing factor study was conducted for 30 days (the study under light condition was conducted for 10 days, the total illumination was not less than 1.2×106 lux·hr). The results showed that the product was stable under light condition (total illuminance 1.2×106 lux/hr) without degradation, and there was no obvious change in various quality indicators. Under high temperature (40° C.), high temperature (60° C.) and 25° C./RH75% conditions, the related substance slightly degraded, but there was no obvious change in other quality indicators, and the sample had a good stability.
  • 3. Product Stability
  • TABLE 9
    Results of product quality study in the stability test (product of Example 1)
    Limit Time
    Study item requirement 0 day 1 month 2 months 3 months
    Appearance A light yellow A Light yellow A Light yellow A Light yellow A Light yellow
    film-coated film-coated film-coated film-coated film-coated
    tablet, being tablet, being tablet, being tablet, being tablet, being
    off-white to off-white off-white off-white off-white
    light yellow after after after after
    after removing removing removing removing removing
    the coating the coating the coating the coating the coating
    Dissolution rate (%) Should be ≥80 97 98 96 99
    Related Impurity A Should be ≤2.0 0.11 0.17 0.19 0.18
    substance Other Should be ≤0.2 0.08 0.06 0.06 0.06
    (%) maximum
    single
    impurity
    Total Should be ≤3.0 0.44 0.38 0.45 0.46
    impurity
    Content (%) Should be 90.0 98.6 100.1 98.8 99.0
    to 110.0
  • TABLE 10
    Results of product quality study in the stability test (product of Example 2)
    Limit Time
    Study item requirement 0 day 1 month 2 month 3 month
    Appearance A pink A pink A pink A pink A pink
    film-coated film-coated film-coated film-coated film-coated
    tablet, being tablet, being tablet, being tablet, being tablet, being
    off-white to off-white off-white off-white off-white
    light yellow after after after after
    after removing removing removing removing removing
    the coating the coating the coating the coating the coating
    Dissolution rate (%) Should be ≥80 98 99 97 99
    Related Impurity A Should be ≤2.0 0.10 0.18 0.27 0.21
    substance Other Should be ≤0.2 0.04 0.02 0.01 0.01
    (%) maximum
    single
    impurity
    Total Should be ≤3.0 0.15 0.22 0.29 0.25
    impurity
    Content (%) Should be 90.0 99.6 99.7 100.9 100.6
    to 110.0
  • It can be seen from Tables 9 and 10 that the tablet prepared in the present invention had a good quality, the product quality was stable in the stability test. The prescription and process of the product were stable, and the reproducibility was good.
  • The samples prepared in other Examples all had similar stability results to those of the samples of Examples 1 and 2.
  • Dissolution Test and Result Analysis
  • 1. Four batches of the product prepared in Example 1 were randomly selected for testing. The results are shown in Table 10.
  • TABLE 11
    Dissolution curve of the product in 0.1M hydrochloric
    acid medium (product of Example 1)
    Product of Example 1 Sample 1 Sample 2 Sample 3 Sample 4
    Cumulative  5 min 51 47 57 76
    dissolution 10 min 94 88 97 97
    rate (%) 15 min 98 94 99 100
    30 min 99 97 100 101
    45 min 100 98 100 102
  • The results showed that the obtained samples all had good dissolution uniformity.
  • 2. The dissolution test of the product prepared in Example 2 in different media was carried out. The results are shown in Table 12.
  • TABLE 12
    Dissolution data of the product in different
    media (product of Example 2)
    Buffer
    0.1 mol/L containing Water
    hydrochloric Buffer, 0.1% SDS, containing
    Dissolution medium acid pH 4.5 pH 6.8 0.1% SDS
    Cumulative  5 min 34 30 66 41
    dissolution 10 min 84 76 86 75
    rate (%) 15 min 97 92 90 87
    30 min 101 96 91 93
    45 min 101 96 92 90
    60 min 101 97 92 94
  • The results showed that the product prepared in the present invention had good dissolution rate in different media.
  • The samples prepared in other Examples had similar dissolution profile to those of the samples of Examples 1 and 2.

Claims (32)

1. A pharmaceutical composition, comprising N-(5-((4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide, an isomer, solvate, hydrate, pharmaceutically acceptable salt thereof or a combination thereof as the active ingredient, and at least one pharmaceutically acceptable excipient.
2. The pharmaceutical composition according to claim 1, wherein the active ingredient is a mesylate salt of N-(5-((4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide.
3. The pharmaceutical composition according to claim 1, wherein the active ingredient is present in an amount of 1 to 60%.
4. The pharmaceutical composition according to claim 3, wherein the unit dose of the active ingredient is 10 to 200 mg.
5. The pharmaceutical composition according to claim 1, wherein the excipient comprises one or more filler(s), comprising at least one disaccharide or polysaccharide, such as glucan, starch, cellulose, lactose, maltose or sucrose.
6. The pharmaceutical composition according to claim 5, wherein the disaccharide or polysaccharide is present in an amount of 1 to 60%; and wherein the filler is a disaccharide.
7. The pharmaceutical composition according to claim 5, wherein the filler also comprises one or more of microcrystalline cellulose, mannitol, sorbitol, calcium hydrophosphate and calcium sulfate.
8. The pharmaceutical composition according to claim 5, wherein the filler is selected from the group consisting of microcrystalline cellulose and lactose.
9. The pharmaceutical composition according to claim 8, wherein the microcrystalline cellulose is present in an amount of 1 to 60%; and the lactose is present in an amount of 1 to 60%.
10. The pharmaceutical composition according to claim 8, wherein the weight ratio of microcrystalline cellulose to lactose is 1:3 to 3:1.
11. The pharmaceutical composition according to claim 5, wherein the filler is selected from the group consisting of microcrystalline cellulose and anhydrous lactose.
12. The pharmaceutical composition according to claim 11, wherein the microcrystalline cellulose is present in an amount of 1 to 60%; and the anhydrous lactose is present in an amount of 1 to 60%.
13. The pharmaceutical composition according to claim 11, wherein the weight ratio of microcrystalline cellulose to anhydrous lactose is 1:3 to 3:1.
14. The pharmaceutical composition according to claim 5, wherein the filler is present in an amount of 20 to 80%.
15. The pharmaceutical composition according to claim 1, wherein the excipient comprises one or more disintegrant(s).
16. The pharmaceutical composition according to claim 15, wherein the disintegrant is one or more selected from the group consisting of low-substituted hydroxypropyl cellulose, croscarmellose sodium, carboxymethyl starch sodium and crospovidone.
17. The pharmaceutical composition according to claim 15, wherein the disintegrant is present in an amount of 1 to 30%.
18. The pharmaceutical composition according to claim 15, wherein the disintegrant is added intragranularly.
19. The pharmaceutical composition according to claim 1, wherein the excipient comprises one or more lubricant(s).
20. The pharmaceutical composition according to claim 19, wherein the lubricant is one or more selected from the group consisting of talc, stearic acid, sodium stearyl fumarate, glyceryl behenate, magnesium stearate and micronized silica gel.
21. The pharmaceutical composition according to claim 19, wherein the lubricant is present in an amount of 0.1 to 10%.
22. The pharmaceutical composition according to claim 19, wherein the lubricant is selected from the group consisting of sodium stearyl fumarate and magnesium stearate, the sodium stearyl fumarate is present in an amount of 0.1 to 5%.
23. The pharmaceutical composition according to claim 1, characterized by comprising the following components:
active ingredient 35 to 50% lactose  5 to 15% microcrystalline cellulose 30 to 50% carboxymethyl starch sodium 10 to 20% lubricant  0.5 to 5%.
24. The pharmaceutical composition according to claim 23, characterized by comprising the following components:
active ingredient 43.3% lactose 10.2% microcrystalline cellulose 30.0% carboxymethyl starch sodium  14% lubricant 0.5 to 5%. 
25. The pharmaceutical composition according to claim 1, wherein the pharmaceutical composition is an oral formulation.
26. (canceled)
27. A method for preparing the pharmaceutical composition according to claim 1, the method comprising:
1) pre-treatment of raw materials: sieving the filler, disintegrant and intragranular lubricant for later use;
2) mixing: weighing the intragranular raw materials according to specified amounts and mixing them;
3) dry granulation: granulating the above mixed powder by dry granulation;
4) total mixing: mixing the resulting granules and extragranular lubricant;
5) optionally, tableting; and
6) optionally, coating.
28. The method according to claim 27, comprising:
1) pre-treatment of raw materials: sieving microcrystalline cellulose, lactose, sodium stearyl fumarate and carboxymethyl starch sodium for later use;
2) mixing: weighing the intragranular raw materials according to prescription amounts, and mixing microcrystalline cellulose, lactose, carboxymethyl starch sodium, sodium stearyl fumarate, the active ingredient and magnesium stearate with a hopper mixer;
3) dry granulation: granulating the above mixed powder with a dry granulator;
4) total mixing: mixing the resulting fine granules and prescription amount of extragranular sodium stearyl fumarate with a hopper mixer;
5) optionally, tableting; and
6) optionally, coating: i) formulation of a coating liquid, adding a prescription amount of Opadry to purified water under stirring to formulate a coating liquid with a solid content of 10%, stirring the coating liquid evenly, and sieving the coating liquid for later use; and ii) finishing the coating until the coating weight gain reaches about 2.0% to 4.0%.
29. The pharmaceutical composition according to claim 6, wherein the disaccharide or polysaccharide is present in an amount of 5 to 15%.
30. The pharmaceutical composition according to claim 6, wherein the filler is lactose.
31. The pharmaceutical composition according to claim 11, wherein the microcrystalline cellulose is present in an amount of 20 to 40%, and the lactose is present in an amount of 5 to 15%.
32. The pharmaceutical composition according to claim 20, wherein the disintegrant is present in an amount of 10 to 20%.
US17/053,605 2018-05-15 2019-05-13 Pharmaceutical composition comprising small molecule egfr inhibitor and preparation method therefor Abandoned US20210069190A1 (en)

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TW202504611A (en) 2023-03-30 2025-02-01 美商銳新醫藥公司 Compositions for inducing ras gtp hydrolysis and uses thereof
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WO2025034702A1 (en) 2023-08-07 2025-02-13 Revolution Medicines, Inc. Rmc-6291 for use in the treatment of ras protein-related disease or disorder
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