US20200022375A1 - Alleviation of corn rootworm damage with microbial seed treatments - Google Patents
Alleviation of corn rootworm damage with microbial seed treatments Download PDFInfo
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- US20200022375A1 US20200022375A1 US16/498,006 US201816498006A US2020022375A1 US 20200022375 A1 US20200022375 A1 US 20200022375A1 US 201816498006 A US201816498006 A US 201816498006A US 2020022375 A1 US2020022375 A1 US 2020022375A1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
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- A01N63/02—
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/08—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A01N63/04—
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
- A01N63/12—Nematodes
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
- A01N63/38—Trichoderma
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- C05G3/02—
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
- C05G5/12—Granules or flakes
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/30—Layered or coated, e.g. dust-preventing coatings
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Definitions
- This disclosure relates to the control of corn rootworms using microbial seed treatments.
- Corn rootworms (“CRW”) ( Diabrotica spp.) are potentially devastating pests in corn production, especially in the corn belt of the United States. Damage exceeds $1 billion per year. Eggs of the pest are deposited in soils in the summer and over winter to hatch in late May or early June. They begin to feed on corn roots and go through three larval instars during their feeding on roots. After feeding for several weeks they molt and the adults winged versions emerge into corn fields. The adults (known as cucumber beetles) feed on corn pollen and silks and may damage silks in conditions of heavy infestation and prevent pollination. They also eat leaves of corn and other crops, and they may carry viruses that infect and damage cucumbers. At the end of the season, they lay eggs in soil that hatch as noted above to feed on corn roots. The primary and greatest damage by these insects is from feeding on corn roots.
- CRW Corn rootworms
- CRW damage to corn roots can offer windows of opportunity for infection by pathogenic fungi in soil.
- nematode damage to roots can cause a significant increase in root damage and function.
- the combined effects of CRW, disease and nematodes all impact the function of roots. Damage to root function not only reduces yields under ideal conditions, but especially under stressful conditions (e.g. drought or soil compaction) the effects are even more devastating.
- the applicant is involved in research to provide products that improve plant performance, including SABREX and GRAPHEX.
- the former product is intended for use with corn (Strain K2 ( T. afroharzianum )+Strain K4 ( T. atroviride )), while the latter is an inoculant seed lubricant used with soybeans.
- FIG. 1 is a chart showing root damage ratings of corn plants grown with several different microbial treatments.
- FIG. 2 is a chart showing plant height of corn plants grown with several different microbial treatments.
- FIG. 3 is a chart showing root weight of corn plants grown with several different microbial treatments.
- Prophylactic insecticide applications may be applied, but they are expensive and only give a positive return to investment to farmers about 15% of the time.
- Other materials such as carbofuran may be applied post-emergence, but timing is critical. If the material is applied too early, the product will leach and degrade before the CRW are present to control. Timed too late and the product may not have time to reach the root zone to control the pest before it damages the crop. This material should be explicitly timed to 1 week before to 1 week after 5% CRW egg hatch (entrance into the 1st instar).
- microbial tools that significantly improve plant performance.
- the microbial tools developed provide abilities to endophytically colonize plant roots. These endophytic microbes can be applied as seed treatments or in numerous other ways that allow them to colonize plant roots where they provide systemic beneficial changes in the plant physiology.
- microbes and products based upon such novel microbial compositions are a preventative biological fungicide and nematicide that enhance plant growth and yield and minimize the effects of root pathogens such as Fusarium, Rhizoctonia and Pythium (examples of parasitic fungi), through control or suppression of the pathogens and through root growth enhancement and that provide nematode control plus systemic disease control that provide control from pathogens such as leaf blights. Effects last throughout the season and provide systemic resistance to stresses such as drought, salt and flooding. These materials are compatible with chemical seed treatments making them very amenable to large-scale agricultural practices.
- Organism 1 Trichoderma virens strain K1 (ATCC 20906) 2 Trichoderma afroharzianum strain K2 (ATCC PTA 9708) 3 Trichoderma afroharzianum strain K3 (ATCC PTA 9709) 4 Trichoderma atroviride strain K4 (ATCC PTA 9707) 5 Trichoderma atroviride strain K5 (NRRL B-50520) 6 Bacillus spp. TR4 7 Bacillus spp. PH1 Untreated control 9 K2 + K4 (SabrEx corn) 10 K2 + K3 (SabrEx wheat)
- Procedure for evaluating western corn rootworm larvae infested on corn seeds provided for a trial. The trial was set up to evaluate the ten different seed treatments.
- the seeds were planted on 26 Jan. 2017 in 4-inch plastic pots with three seeds per pot. These pots were placed in the green house to get fast germination and growth. On 8 Feb., each pot was thinned to two plants. The next day, each was infested with 25 neonate western corn rootworm non-diapausing larvae, which is the only type of corn rootworm larvae that Applicant was allowed to use in these types of studies by the US Animal and Plant Health Inspection Service.
- each plot was evaluated for the number of corn rootworms surviving, the instar of each larvae, the plant height, the plant root length and the weight of each root mass in grams.
- the length of time from 9 February to 23 February is the approximate time for western corn rootworm larvae to go through three larval instars. At this time, the CRW should be near the prepupal stage of development.
- FIG. 1 is a chart showing root damage ratings of corn plants grown with several different microbial treatments. Most of the treatments showed reduced root damage.
- FIG. 2 is a chart showing plant height of corn plants grown with several different microbial treatments. Many of the treatments increased plant heights in the presence of root damage caused by the CRW.
- FIG. 3 is a chart showing root weight of corn plants grown with several different microbial treatments. In most of the cases, treated root weights were greater than the untreated control root weights in the presence of CRW.
- Treatment with the disclosed organisms on seeds alleviated damage on roots, even though the number of larvae on roots were not reduced. This is indicative of a mechanism by which tolerance is induced in the corn to CRW.
- the microbial organisms used for treatment also increased root and plant growth and, at the same time, reduced damage to roots by pathogens and nematodes.
- Microbial treatment methods other than seed treatments are also contemplated by this disclosure. Since the disclosed treatment methods are effective as a seed and as a foliar treatment, there are various other embodiments and applications that are effective, such as, e.g., including: applications as an in-furrow granule; application as a soil drench where the organism will come into contact with roots and colonize roots; application as a root treatment, e.g, during transplant operations; and as a component of liquid or solid fertilizers.
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Priority Applications (1)
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US16/498,006 US20200022375A1 (en) | 2017-03-30 | 2018-03-30 | Alleviation of corn rootworm damage with microbial seed treatments |
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US201762479074P | 2017-03-30 | 2017-03-30 | |
US201762479084P | 2017-03-30 | 2017-03-30 | |
US201762479080P | 2017-03-30 | 2017-03-30 | |
US16/498,006 US20200022375A1 (en) | 2017-03-30 | 2018-03-30 | Alleviation of corn rootworm damage with microbial seed treatments |
PCT/US2018/025592 WO2018183978A1 (en) | 2017-03-30 | 2018-03-30 | Alleviation of corn rootworm damage with microbial seed treatments |
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US16/498,006 Abandoned US20200022375A1 (en) | 2017-03-30 | 2018-03-30 | Alleviation of corn rootworm damage with microbial seed treatments |
US16/497,975 Active 2038-04-02 US11930812B2 (en) | 2017-03-30 | 2018-03-30 | Coating materials for seeds and for particulate materials, including fertilizers |
US16/639,070 Active 2038-08-09 US11464227B2 (en) | 2017-03-30 | 2018-03-30 | Enhanced microbial and biorational control of nematode pests of plants |
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US16/497,975 Active 2038-04-02 US11930812B2 (en) | 2017-03-30 | 2018-03-30 | Coating materials for seeds and for particulate materials, including fertilizers |
US16/639,070 Active 2038-08-09 US11464227B2 (en) | 2017-03-30 | 2018-03-30 | Enhanced microbial and biorational control of nematode pests of plants |
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US (3) | US20200022375A1 (de) |
EP (3) | EP3599866A4 (de) |
AU (1) | AU2018244940A1 (de) |
BR (3) | BR112019020481A2 (de) |
CA (1) | CA3058464A1 (de) |
MX (3) | MX2019011716A (de) |
WO (3) | WO2018183976A1 (de) |
Cited By (1)
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WO2022098686A1 (en) * | 2020-11-06 | 2022-05-12 | Agrauxine Corp. | Corn rootworm biological control |
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BR112020008226A2 (pt) | 2017-10-25 | 2020-10-27 | Advanced Biological Marketing, Inc. | método de formulação agrícola e química de micróbio combinado e composição derivativa por micróbio, e uso da mesma |
MX2020004521A (es) | 2017-10-25 | 2020-12-07 | Advanced Biological Marketing Inc | Formulaciones de tratamiento microbiano endofítico para semillas y métodos relacionados con las mismas para el desempeño mejorado de plantas. |
BR112020010074A2 (pt) * | 2017-11-21 | 2020-11-03 | Advanced Biological Marketing, Inc. | composições e métodos para induzir alterações de cultura equilibrando os efeitos de um produto químico agrícola aplicado |
WO2020102420A1 (en) | 2018-11-14 | 2020-05-22 | Anuvia Plant Nutrients Holdings, Llc | Delivery of bioactive molecules in coatings or surface layers of organically enhanced inorganic fertilizers |
US11407690B2 (en) * | 2019-03-29 | 2022-08-09 | Cytozyme Laboratories, Inc. | Plant fertilizer compositions and related methods |
CN110810174B (zh) * | 2019-11-15 | 2021-12-24 | 山东省科学院生态研究所(山东省科学院中日友好生物技术研究中心) | 哈茨木霉ltr-2在禾谷作物栽培中的应用 |
US20210321625A1 (en) * | 2020-04-17 | 2021-10-21 | Advanced Biological Marketing, Inc. | Method of stable formulation of microbial and microbe derived products and use of same |
CN112322501B (zh) * | 2020-11-20 | 2022-04-15 | 山东省科学院生态研究所(山东省科学院中日友好生物技术研究中心) | 一株非洲哈茨木霉Ta97及其在秸秆还田方面的应用 |
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EP3205209A1 (de) * | 2016-02-09 | 2017-08-16 | Basf Se | Mischungen und zusammensetzungen mit paenibacillus-stämmen oder metaboliten davon und anderen biopestiziden |
MX2018013444A (es) * | 2016-05-02 | 2019-09-05 | Advanced Biological Marketing Inc | Composiciones microbianas altamente efectivas y multifuncionales y usos. |
MX2019011716A (es) * | 2017-03-30 | 2019-12-16 | Advanced Biological Marketing Inc | Control aumentado microbiano y bioracional de plagas de nematodos de plantas. |
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2018
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- 2018-03-30 BR BR112019020483A patent/BR112019020483A2/pt not_active Application Discontinuation
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2022098686A1 (en) * | 2020-11-06 | 2022-05-12 | Agrauxine Corp. | Corn rootworm biological control |
US11968982B2 (en) | 2020-11-06 | 2024-04-30 | Agrauxine Corp. | Corn rootworm biological control |
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BR112019020482A2 (pt) | 2020-04-28 |
US11930812B2 (en) | 2024-03-19 |
MX2019011716A (es) | 2019-12-16 |
WO2018183978A1 (en) | 2018-10-04 |
BR112019020481A2 (pt) | 2020-04-28 |
US20200221705A1 (en) | 2020-07-16 |
WO2018183976A1 (en) | 2018-10-04 |
BR112019020482B8 (pt) | 2024-02-15 |
EP3599861A4 (de) | 2020-12-30 |
US11464227B2 (en) | 2022-10-11 |
BR112019020483A2 (pt) | 2020-04-28 |
WO2018183977A1 (en) | 2018-10-04 |
AU2018244940A1 (en) | 2019-10-31 |
MX2019011560A (es) | 2019-11-21 |
EP3599866A1 (de) | 2020-02-05 |
EP3599865A4 (de) | 2021-01-20 |
MX2019011561A (es) | 2019-11-18 |
CA3058464A1 (en) | 2018-10-04 |
EP3599865A1 (de) | 2020-02-05 |
EP3599866A4 (de) | 2021-04-07 |
BR112019020482B1 (pt) | 2024-01-16 |
EP3599861A1 (de) | 2020-02-05 |
US20210101847A1 (en) | 2021-04-08 |
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