US20190185829A1

US20190185829A1 - Preparation of lipase with improved ester synthesis activity by using surfactants

Info

Publication number: US20190185829A1
Application number: US16/284,511
Authority: US
Inventors: Dong Wang; Yan Xu
Original assignee: Jiangnan University
Current assignee: Jiangnan University
Priority date: 2017-06-08
Filing date: 2019-02-25
Publication date: 2019-06-20
Also published as: WO2018223664A1; CN107034205B; CN107034205A; US20210017505A1; US11214778B2

Abstract

The present disclosure discloses a method for preparing lipase with high ester synthesis activity by using surfactant, belonging to the field of enzyme engineering. The present disclosure provides a method for obtaining a lipase with high ester synthesis activity by adding different surfactants with different concentrations in a lipase aqueous solution and then freeze-drying. The lipase meets the requirement in non-aqueous catalysis. Mixing a variety of lipase with no ester synthesis activity or low activity at suitable concentration and an appropriate concentration of the surfactant in the solution can produces lipases with significantly improved ester synthesis activity, meanwhile changing the hydrolytic activity of the lipase. Increased ester synthesis activity makes lipase more suitable for industrial applications.

Description

TECHNICAL FIELD

The disclosure herein relates to the preparation of lipase with improved ester synthesis activity by using surfactants, which belongs to the field of enzyme engineering.

BACKGROUND

Lipase (triacylglycerol ester hydrolases, EC 3.1.1.3), firstly reported in 1901, is a class of biocatalysts hydrolyzing long-chain triacylglycerol ester at the water-oil interface and widely exists in animals, plants and microorganisms. Besides the hydrolysis of ester bonds, lipases can catalyze esterification and transesterification reaction in non aqueous environments and therefore gain great interest in the field of biological chemical industry. As being able to facilitate very special chemical transformation, lipases are gaining more and more interest in several industries such as food, detergents, cosmetics, organic synthesis, pharmaceutical. For example, they can be used in progressing food, wastewater treatment, synthesis of biological surfactants, treatment of wood cellulose pulp paper resin and biosynthesis of chiral drugs. Lipases also have advantages of good stability and high conversion efficiency and they have application value in the production of aromatic esters, biodiesel and chiral compounds.
Most lipases can catalyze hydrolysis of ester bonds. However, only few catalyzes ester synthesis reaction in non-aqueous media and most microbial lipases do not display apparent activity when catalyzing ester synthesis reaction in non-aqueous media. Several heterologous expressed lipases display only remarkable hydrolysis activity but little ability to catalyze synthesis of esters in comparison with wild-type lipases. The industrial application of lipases is hampered by their poor activities catalyzing ester synthesis reaction.
Several protein-modified methods, such as enzyme immobilization and pretreatment with organic solvent, have been adapted to adjust or improve activity of enzyme. But these methods cannot change catalytic performance of lipases essentially. Besides, surfactants can adjust catalytic performance and generally improve the lipase activity of hydrolysis. Employing surfactants to enhance lipase activity in non-aqueous media have also been reported decades ago, including formation of surfactant-coated enzyme or reverse micelle system, both of which will improve the solubility of enzymes in non-aqueous media, and molecular bio-imprinting technologies—lyophilize lipases in the presence of surfactants and wash them off afterwards. Although sometimes effective, these methods are complicated and time-consuming in practice and cannot be applicable to all the lipases. Developing a straightforward, versatile method to improve the activity of lipase catalyzing ester synthesis in non-aqueous has a significant practical value.

SUMMARY

To solve the problems above, the present disclosure provides a method for preparation of the lipases meeting the requirement of practical application in non-aqueous catalysis, which have high ester synthesis activity, by changing microstructures of lipases via the interaction between surfactants and soluble lipases in aqueous environments and lyophilizing mixture afterwards. The present disclosure also provides a method which can be applicable to other lipases.
The present disclosure provides a method for enhancing ester synthesis activity of lipases by using surfactants. The present disclosure relates to modify lipases by adding surfactants and obtain lipases by lyophilization, which are able to catalyze synthesis of esters.
The present disclosure solves the problem that many recombinant lipases do not display ester synthesis activity, and lays the foundation for expanding the industrial application of lipase. The present disclosure is obviously different from the existing methods for modifying lipase by using surfactants to obtain high ester synthesis activity, and has good operability and wide applicability.
The method for enhancing ester synthesis activity of lipases is to directly add surfactants to the lipase solution and mix afterwards, which can provide a hydrophobic environment enabling soluble lipases to regain the ester synthesis activity by changing the microstructure of lipases.
In an embodiment of the present disclosure, the ester synthesis activity described refers to the activity of catalyzing synthesis of esters in a non-aqueous phase.
In an embodiment of the present disclosure, the aqueous solution of the lipase described refers to the solution obtained by dissolving the lipase (powder) directly in an aqueous solution.
In an embodiment of the present disclosure, the aqueous solution described can be water and other buffer solutions.
In an embodiment of the present disclosure, the buffer solution can be phosphate buffer, citrate buffer, acetate buffer, Tris buffer and the like.
In an embodiment of the present disclosure, the concentration of buffer solution ranges from 25 to 100 mmol·L⁻¹pH 6.5-8.0.
In an embodiment of the present disclosure, the buffer is a 25 mmol·L⁻¹phosphate buffer pH 7.5.
In an embodiment of the disclosure, the lipases described include, but are not limited to, Rhizopus chinensis lipase r27RCL, Rhizoupus oryzae lipase ROL, Pseudomonas cepacia lipase PCL, Candida antarctica lipase CALB, and the like.
In an embodiment of the disclosure, the lipase can be a lipase that is heterologously expressed, a lipase expressed by a recombinant microorganism, or any other lipases that have lower or no ester synthesis activity relative to the wild-type lipase.
In an embodiment of the present disclosure, the protein concentration of the pure enzyme in the soluble lipase solution described ranges from 0.1 and 0.4 mg·mL⁻¹to ensure that lipases interact with surfactants completely.
In an embodiment of the present disclosure, the surfactant described can be one or a combination of a zwitterionic surfactant, a nonionic surfactant, a cationic surfactant, and the like. Different surfactants have different effects on different lipases. But surfactants described are not limited to the above-mentioned surfactants.
In an embodiment of the present disclosure, the zwitterionic surfactants are 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), polyethylene glycol octylphenyl Ether (Triton X-100), 1-myristyl 2-acyl-cis-propyltri-phosphatidylcholine (LPC14), 1,2-dihexanoyl lecithin (DiC6PC), 1-(LPC16), 1-Lauramate-2-acyl-cis-propyltri-phosphatidylcholine (LPC16), 1-myristyl-2-acyl-cis (LPG12), 1-caramom-2-acyl-cis-propyltriyl-phosphoric acid-1-glycerol (LPG14), 1-palm-2-acyl-cis (LPG16), 1,2-diheptanoyl-S-glycero-3-phosphorylcholine (DHPC), or a combination of two or more.
In an embodiment of the present disclosure, the nonionic surfactants described are n-dodecyl-ß-D-maltopyranoside (DDM), octaethylene glycol monododecyl ether (C12E8), n-decyl-ß-D-maltopyranoside (DM), n-undecyl-ß-D-maltopyranoside (UDM), n-octyl-ß-D-glucopyranoside (OG), octaethylene glycol monodecyl ether (C10E8), or a combination of two or more.
In an embodiment of the present disclosure, the cationic surfactants described are hexadecyl trimethyl ammonium bromide (CTAB) and the like.
In an embodiment of the present disclosure, the surfactant aqueous solution can be stored at 4° C. for a short time.
In an embodiment of the present disclosure, the protein solution and the surfactant solution are mixed at a volume ratio ranging from 1:0.5 to 1:2.
In an embodiment of the present disclosure, the protein solution and the surfactant solution are mixed at a volume ratio of 1:1.
In an embodiment of the present disclosure, the final concentration of the surfactant ranges from 0.1 times the critical micelle concentration (0.1×CMC) to 200 times the critical micelle concentration (200×CMC).
In an embodiment of the present disclosure, the final concentration of the surfactant ranges from 10 times the critical micelle concentration (10×CMC) and 200 times the critical micelle concentration (200×CMC).
In an embodiment of the present disclosure, the final concentration of the surfactant is 100 times the critical micelle concentration (100×CMC).
In an embodiment of the present disclosure, the amount of surfactant added is high enough to make the final concentration range from 1 to 500 mM.
In an embodiment of the present disclosure, the amount of surfactant added is high enough to make the final concentration reach 10 mM.
In an embodiment of the present disclosure, the addition of surfactants can further enhance ester synthesis activity and/or total recovery of ester synthesis activity of Rhizopus chinensis lipase r27RCL, Rhizoupus oryzae lipase ROL, Pseudomonas cepacia lipase PCL, Candida antarctica lipase CALB and the like.
In an embodiment of the present disclosure, the method comprises the step of removing the water after mixing.
The second object of the present disclosure is to provide a lipase preparation with improved activity catalyzing ester synthesis in a non-aqueous phase, wherein the lipase preparation is prepared by dissolving a lipase in an aqueous solution, directly adding a surfactant, mixing, and then removing the water.
In an embodiment of the present disclosure, the step of removing the water described can be the drying, and lyophilizaton.
In an embodiment of the present disclosure, the aqueous solution described can be water and other buffer solutions.
In an embodiment of the disclosure, the lipases described can be a lipase that is heterologously expressed, a lipase expressed by a recombinant microorganism, or any other lipases that have lower or no ester synthesis activity relative to the wild-type lipase.
The third object of the present disclosure is to provide the application of the lipase described in ester synthesis reaction.
In an embodiment of the present disclosure, the ester synthesis reaction is a ester synthesis reaction in non-aqueous phase.
The fourth object of the present disclosure is to provide the application of lipases described in the field of food, chemical, biological, pharmaceutical, environmental, and petroleum.
In an embodiment of the present disclosure, the application includes, but is not limited to, detergents production, cosmetics production, wastewater treatment, bio-surfactants production, wood cellulose pulp paper resins treatment, biosynthesis of chiral drugs, aromatic ester production, biodiesel production, chiral compound production and the like.

Beneficial Effect of the Present Disclosure

(1) Conformation and function of protein are closely related to the environments around. The present disclosure provides a method of producing lipases with high ester synthesis activity by in vitro adjustment of lipase activity by simulating cellular membrane hydrophobic environment using surfactants.
(2) The method of the present disclosure is applicable to Rhizopus chinensis lipase r27RCL expressed by Pichia pastoris, commercial Rhizoupus oryzae lipase ROL, commercial Pseudomonas cepacia lipase PCL, commercial Candida antarctica lipase CALB and the like. The method described can help lipase regain ester synthesis activity or increase ester synthesis activity by 8-30 times, and enable the total recovery of lipase ester synthesis activity reach 105%-1733%.

DETAILED DESCRIPTION

Determination of Lipase Activity
(1) Determination of Hydrolytic Activity of the Lipase Using p-Nitrophenyl Palmitate (pNPP) as Substrate
Hydrolysis of pNPP catalyzed by lipase generates p-nitrophenol and palmitic acid. p-nitrophenol appears yellow in the buffer at pH 8.0 with the maximum absorption peak at 410 nm. Determination of hydrolytic activity can be achieved by measuring the absorbance at 410 nm.
The substrate solution A: 50 mmol·L⁻¹sodium phosphate buffer (pH 8.0) containing 1.16 g·L⁻¹sodium deoxycholate and 0.56 g·L⁻¹arabic gum. Substrate solution B: 0.015 g pNPP is dissolved in 5 mL isopropyl alcohol. Substrate solution A and substrate solution B are mixed then stored for use afterwards.
Termination solution: 40 g·L⁻¹NaOH, 93.05 g·L⁻¹EDTA sodium. Add 62 uL termination solution to the reaction mixture when stopping the reaction.
Determination Method:
Add 0.1 mL appropriately diluted enzyme solution, which is substituted by inactivated enzyme solution as the control, to 2.4 mL substrate solution above, incubate the mixture at 40° C. for 2 min and measure the absorbance at 410 nm.
Definition of Enzyme Activity:
one hydrolytic activity unit is defined as the amount of enzyme that catalyzes the formation of 1 μmol of p-nitrophenol in 1 minute at 40° C.
enzyme activity (U·mL⁻¹)=(V×A ₄₁₀×10⁶)/(ε×t×V′) Calculation equation:
where V is the volume of the reaction mixture (mL), E is the molar extinction coefficient (mL·mmol⁻¹), t is the reaction time (min) and V′ is the volume of the enzyme solution (mL).
(2) Determination of Ester Synthesis Activity of the Lipase by GC Analysis
Reaction Substrates:
Substrate solution A: 48.5 mL of n-octanoic acid is dissolved in 250 mL of n-heptane in a volumetric flask.
Substrate solution B: 17.5 mL of absolute ethanol is dissolved in 250 mL of n-heptane in a volumetric flask.
Internal standard: 2-hexanol/n-heptane (35 g·L⁻¹).
Standard sample: 10 g of n-octanoate is dissolved in 1000 mL of n-heptane in a volumetric flask.
Method of Determination
1 mL substrate solution A and substrate solution B are added in a 5 mL Eppendorf tube respectively, then 20 mg lipase powder (or lyophilized powder) is added. The reaction is carried out at 40° C. and with shaking at 150 rpm for 30 min. Remove the lipase powder by centrifugation or membrane filtration. Then 0.1 mL of internal standard is added in 0.4 mL of filtrate or supernatant and mixed. Measure the content of n-octanoate in the mixture above by GC analysis.
The gas chromatograph (6820, Agilent Instruments) is equipped with a AC20 (PEG20000) capillary column and a FID detector. Nitrogen was used as the carrier gas. The oven temperature was programmed to start at 90° C. for 1 min and then be elevated to 200° C. for 5 min at 10° C.·min⁻¹. The injector and detector temperatures were set at 250° C.
Definition of enzyme activity: One unit of ester synthesis activity is defined as the amount of enzyme that esterifies 1 micromole of n-octanoate per min.
Calculation Equation:
$enzyme activity (U \cdot {mg}^{- 1}) = \frac{A_{sam}}{A_{sta}} \times S_{sta} \times V \times 10^{6} \times \frac{1}{172} \times \frac{1}{30} \times \frac{1}{m}$
A_sam—the ratio of the peak areas of the sample for testing to the internal standard;
A_sta—the ratio of the peak areas of the standard sample to the internal standard;
S_sta—the concentration of the standard sample (g·L⁻¹);
V—the volume of the reaction mixture (L);
m—the amount of lipase in the reaction mixture (mg).
Specific activity: the activity of lipase per milligram of total protein in the lipase preparation.
Total recovery: the percentage of the activity after a treatment to that before the treatment

Example 1: Method of Operation for Enhancing Lipase Ester Synthesis Activity

Add an equal volume of high-concentration surfactant solution into the enzyme solution. The final protein concentration was 0.25 mg·mL⁻¹and the surfactant concentration was 10 mM. After mixed thoroughly, the mixture was lyophilized and kept in dry condition.

Example 2: Regain of the Ester Synthesis Activity of Commercial r27RCL

Commercial lipase r27RCL was purchased from Jiangsu Yiming Biological Technology Co., Ltd, which has high hydrolytic activity and low ester synthesis activity. Dissolve the r27RCL powder in water and centrifuge to obtain the supernatant containing the r27RCL lipase to adjust the ester synthesis activity (the final protein concentration was 0.2 mg·mL⁻¹and the final concentration of surfactant was 10 mM, which is between 0.1×CMC^˜200×CMC of the different surfactants in Table 1). As shown in Table 1, addition of LPC14 increased the ester synthesis specific activity from 5.5 U·mg⁻¹to 47.6 U·mg⁻¹and the total recovery of ester synthesis activity was 769% while the hydrolytic specific activity did not show significant change.

TABLE 1

Ester synthesis activity and hydrolytic activity of r27RCL.

	Specific activity	Total recovery
surfactant	(U · mg⁻¹)	(%)

Ester	blank	5.5	100
synthesis	CTAB	0	0
activity	DiC6PC	15.9	256
	C12E8	22.2	372
	LPC14	47.6	769
	DDM	31.4	423
	CHAPS	13.0	105
	Triton X-100	12.9	192
Hydrolytic	blank	115.7	100
activity	CTAB	0	0
	DiC6PC	104.2	87
	C12E8	117.1	101
	LPC14	116.1	97
	DDM	135.0	94
	CHAPS	132.6	109
	Triton X-100	116.6	93

Example 3: Regain of the Ester Synthesis Activity of Commercial ROL

Commercial lipase ROL was purchased from Sigma-Aldrich Co., Ltd. ROL is a non-immobilized lipase. Dissolve the ROL powder in water and centrifuge to obtain the supernatant containing the ROL lipase that would be regulated to improve its ester synthesis activity by adjusting the final protein concentration to 0.2 mg·mL⁻¹and adding surfactants to the final concentration of 10 mM. As shown in Table 2, addition of LPC14 increased its ester synthesis specific activity from 0.4 U·mg⁻¹to 12.2 U·mg⁻¹and the total recovery of ester synthesis activity was 1733%.

TABLE 2

Ester synthesis activity and hydrolytic activity of ROL.

	Specific activity	Total
surfactant	(U · mg⁻¹)	recovery (%)

Eester	blank	0.4	100
synthesis	CTAB	0	0
activity	DiC6PC	3.11	733
	C12E8	2.6	400
	LPC14	12.2	1733
	DDM	2.8	333
	CHAPS	2.6	400
	Triton X-100	1.7	200
Hydrolytic	blank	18.5	100
activity	CTAB	0	0
	DiC6PC	15.2	76
	C12E8	23.7	82
	LPC14	33.1	106
	DDM	42.7	116
	CHAPS	23.6	81
	Triton X-100	34.9	99

Example 4: Regain of the Ester Synthesis Activity of Commercial PCL

Commercial lipase PCL was purchased from Sigma-Aldrich Co., Ltd. PCL is a non-immobilized lipase. Dissolve the PCL powder in water and centrifuge to obtain the supernatant containing the PCL lipase that would be regulated to improve the ester synthesis activity by adjusting the final protein concentration to 0.2 mg·mL⁻¹and adding surfactants to the final concentration of 10 mM. As shown in Table 3, addition of DDM increased its ester synthesis specific activity from 2.5 U·mg⁻¹to 55.6 U·mg⁻¹and the total recovery of ester synthesis activity was 1435%.

TABLE 3

Ester synthesis activity and hydrolytic activity of PCL.

	Specific activity	Total
surfactant	(U · mg⁻¹)	recovery (%)

Ester	Blank	2.5	100
synthesis	CTAB	0	0
activity	DiC6PC	7.1	260
	C12E8	4.6	130
	LPC14	44.6	1260
	DDM	55.6	1435
	CHAPS	4.7	130
	Triton X-100	6.3	174
Hydrolytic	blank	214.8	100
activity	CTAB	75.5	29
	DiC6PC	308.2	140
	C12E8	281.1	98
	LPC14	321.8	119
	DDM	327.7	111
	CHAPS	248.0	101
	Triton X-100	364.5	131

Example 5: Increase of the Ester Synthesis Activity of Commercial CALB

Commercial lipase CALB was purchased from Sigma-Aldrich Co., Ltd. CALB is a non-immobilized lipase. Dissolve the CALB powder in water and centrifuge to obtain the supernatant containing the CALB lipase that would be regulated to improve the ester synthesis activity by adjusting the final protein concentration to 0.12 mg·mL⁻¹and adding surfactants to the final concentration of 10 mM. As shown in Table 4, addition of DDM increased the ester synthesis specific activity from 7.8 U·mg⁻¹to 101.9 U·mg⁻¹and the total recovery of ester synthesis activity was 700%.

TABLE 4

Ester synthesis activity and hydrolytic activity of CALB.

	Specific activity	Total
surfactant	(U · mg⁻¹)	recovery (%)

Ester	Blank	7.8	100
synthesis	CTAB	36.6	300
activity	DiC6PC	31.9	400
	C12E8	62.8	467
	LPC14	36.8	411
	DDM	101.9	700
	CHAPS	5.9	44
	Triton X-100	77.4	544
Hydrolytic	blank	1.1	100
activity	CTAB	0	0
	DiC6PC	0.98	85
	C12E8	0	0
	LPC14	0.54	38
	DDM	0	0
	CHAPS	1.6	92
	Triton X-100	0	0

Example 6: Effect of Concentration of Surfactant LPC14 on Ester Synthesis Activity of Commercial r27RCL

Commercial lipase r27RCL was purchased from Jiangsu Yiming Biological Technology Co., Ltd. r27RCL is a lipase with high hydrolytic activity but low ester synthesis activity. Dissolve the r27RCL powder in water, centrifuge to obtain the supernatant and treat the supernatant with different concentrations of surfactant LPC14 (the final protein concentration was 0.2 mg·mL⁻¹). As shown in Table 5, ester synthesis activity of r27RCL obtained more improvement at higher concentration of surfactant LPC14 (100×CMC).

TABLE 5

Effect of concentration of LPC14 on ester synthesis activity
and hydrolytic activity of r27RCL.

concentration	Specific activity	Total
(x CMC)	(U · mg⁻¹)	recovery (%)

Ester synthesis	0	5.5	100
activity	0.1	1.8	32
	1	1.4	25
	100	17.1	292
Hydrolytic	0	115.7	100
activity	0.1	120.2	101
	1	122.2	102
	10	128.0	107
	100	125.7	102

Example 7: Effect of Concentration of Surfactant LPC14 on Ester Synthesis Activity of Commercial ROL

Commercial lipase ROL was purchased from Sigma-Aldrich Co., Ltd. and is a non-immobilized lipase. Dissolve the ROL powder in water, centrifuge to obtain the supernatant and treat the supernatant with different concentrations of surfactant LPC14 (the final protein concentration was 0.2 mg·mL⁻¹). As shown in Table 6, ester synthesis activity obtained more improvement at higher concentration of surfactant LPC14 (10×-100×CMC).

TABLE 6

Effect of concentration of LPC14 on ester synthesis activity
and hydrolytic activity of ROL.

concentration	Specific activity	Total
(x CMC)	(U · mg⁻¹)	recovery (%)

Ester synthesis	0	0.4	100
activity	0.1	0.4	97
	1	0.2	46
	10	1.0	240
	100	6.2	1517
Hydrolytic	0	18.5	100
activity	0.1	28.7	150
	1	27.7	139
	10	31.2	162
	100	39.0	206

Example 8: Effect of Concentration of Surfactant DDM on Ester Synthesis Activity of Commercial PCL

Commercial lipase PCL was purchased from Sigma-Aldrich Co., Ltd. and is a non-immobilized lipase. Dissolve the PCL powder in water, centrifuge to obtain the supernatant and treat the supernatant with different concentrations of surfactant DDM (the final protein concentration was 0.2 mg·mL⁻¹). As shown in Table 7, ester synthesis activity obtained more improvement at higher concentration of surfactant DDM (10×-100×CMC).

TABLE 7

Effect of concentration of DDM on ester synthesis activity
and hydrolytic activity of PCL

concentration	Specific activity	Total
(x CMC)	(U · mg⁻¹)	recovery (%)

Ester synthesis	0	2.5	100
activity	0.1	3.2	58
	1	2.9	57
	10	20.0	260
	100	101.6	2776
Hydrolytic	0	214.8	100
activity	0.1	499.5	106
	1	434.7	99
	10	756.7	114
	100	773.5	246

Example 9: Effect of Concentration of Surfactant DDM on Ester Synthesis Activity of Commercial CALB

Commercial lipase CALB was purchased from Sigma-Aldrich Co., Ltd. and is a non-immobilized lipase. Dissolve the CALB powder in water, centrifuge to obtain the supernatant and treat the supernatant with different concentrations of surfactant DDM (the final protein concentration was 0.12 mg·mL⁻¹). As shown in Table 8, ester synthesis activity obtained more improvement at higher concentration of surfactant DDM (100×CMC).

TABLE 8

Effect of concentration of DDM on ester synthesis activity
and hydrolytic activityof CALB.

concentration	Specific activity	Total
(x CMC)	(U · mg⁻¹)	recovery (%)

Ester synthesis	0	7.8	100
activity	0.1	4.0	12
	1	14.3	50
	10	37.3	73
	100	32.6	189
Hydrolytic	0	1.1	100
activity	0.1	2.1	45
	1	3.0	75
	10	0.7	10
	100	0	0

In summary, the present disclosure obtains the lipase preparation with high ester synthesis activity by in vitro regulating the activity of the lipase by simulating the hydrophobic microenvironment around the protein using surfactants. The ester synthesis activity of the lipase r27RCL expressed by Pichia pastoris was increased from 5.5 U·mg⁻¹to 47.6 U·mg⁻¹and the total ester synthesis activity recovery was 769% after the surfactant treatment. The ester synthesis activity of commercial ROL was increased from 0.4 U·mg⁻¹to 12.2 U·mg⁻¹, and the total activity recovery was 1733% after the surfactant treatment. The ester synthesis activity of commercial PCL was increased from 2.5 U·mg⁻¹to 55.6 U·mg¹, and the total activity recovery was 1435% after the surfactant treatment. The ester synthesis activity of commercial CALB was increased from 7.8 U·mg⁻¹to 101.9 U·mg¹, and the total activity recovery was 700% after the surfactant treatment. The concentrations of surfactant used significantly affected the regain of lipase ester synthesis activity. The higher concentration (10×-100×CMC) of the surfactant can result in better lipase ester synthesis activity. The increase of both the specific activity and total activity recovery indicated that the activity regulation by simulating the hydrophobic environment of cell membrane in vitro had a positive effect on the ester synthesis activity of lipase. This method was not only applicable to r27RCL, but also to other lipases.
The disclosure described and claimed herein is not to be limited in scope by the specific aspects herein disclosed. Any person skilled in the art can make modifications without departing from the spirit and scope of the disclosure. The scope of protection of the present disclosure should therefore be defined by the claims.

Claims

What is claimed is:

1. A method for increasing ester synthesis activity of lipase, comprising adding surfactants directly to aqueous solution of soluble lipase and mixing afterwards.

2. The method for increasing the ester synthesis activity of lipase according to claim 1, wherein the aqueous solution of the lipase is obtained by dissolving a lipase powder directly in the aqueous solution.

3. The method for increasing the ester synthesis activity of lipase according to claim 1, wherein the aqueous solution is phosphate buffer saline or Tris-HCl buffer.

4. The method for increasing the ester synthesis activity of lipase according to claim 1, wherein the surfactant is one or a combination of zwitterionic surfactants, nonionic surfactants and cationic surfactants.

5. The method for increasing the ester synthesis activity of lipase according to claim 1, wherein the soluble lipase comprises Rhizopus chinesis lipase r27RCL, Rhizopus oryzae lipase ROL, Pseudomonas cepacia lipase PCL, or Candida antarctica lipase CALB.

6. The method for increasing the ester synthesis activity of lipase according to claim 1, wherein the soluble lipase is a lipase that is heterologously expressed, a lipase expressed by a recombinant microorganism, or any other lipases that have lower or no ester synthesis activity relative to a wild-type lipase.

7. The method for increasing the ester synthesis activity of lipase according to claim 1, wherein protein concentration of pure lipase in the aqueous solution of the soluble lipase is between 0.1 and 0.4 mg·mL⁻¹.

8. The method for increasing the ester synthesis activity of lipase according to claim 4, wherein the zwitterionic surfactants comprises 3-[3-(cholamidopropyl) dimethylamino] propanesulfonic acid internal salt, polyethylene glycol octyl Phenyl ether, 1-myristyl 2-acyl-cis-propyltriyl-phosphatidylcholine, 1,2-dihexanoyl lecithin, 1-myristyl-2-acyl-cis-propyltri-2-acyl-cis-propyltriate-phosphatidylcholine, 1-myristyl-2-acyl-cis-propyltriyl-phosphonic acid-1-glycerol, 1-myrist 2-acyl-cis-propyltri-phospho-1-glycerol or 1,2-diheptanoyl-S-glyceryl, Glycerol-3-phosphorylcholine; the nonionic surfactant is dodecyl-ß-D-maltoside, octylglycol monobutyl dodecyl ester, decyl-ß-D-maltose Glycoside, undecyl-ß-D-maltoside, octyl-ß-D-glucopyranoside or polyoxypropylene glycol monethylether.

9. The method for increasing the ester synthesis activity of lipase according to claim 4, wherein the nonionic surfactants comprises n-dodecyl-ß-D-maltopyranoside, octaethylene glycol monododecyl ether, n-decyl-ß-D-maltopyranoside, n-undecyl-ß-D-maltopyranoside, n-octyl-ß-D-glucopyranoside or octaethylene glycol monodecyl ether.

10. The method for increasing the ester synthesis activity of lipase according to claim 1, wherein final concentration of surfactant is ranging from 0.1 times to 200 times critical micelle concentration and above.

11. The method for increasing the ester synthesis activity of lipase according to claim 1, wherein the addition of the surfactant is 1-500 mM or higher at final concentration.

12. A lipase preparation with improved ester synthesis activity in non-aqueous phase, comprising a lipase preparation obtained by dissolving the lipase in an aqueous solution and then adding surfactant directly to the solution followed by removing water.

13. A method comprising adding the lipase preparation according to claim 12 as a catalyst in catalyzing ester synthesis reaction in non-aqueous phase; or adding the lipase preparation according to claim 12 as a catalyst in catalyzing a reaction in field of foodstuffs, chemistry, biology, preparation of medicine, or environment or petroleum field.