US20180186871A1 - Il-11 antibodies - Google Patents

Il-11 antibodies Download PDF

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US20180186871A1
US20180186871A1 US15/843,173 US201715843173A US2018186871A1 US 20180186871 A1 US20180186871 A1 US 20180186871A1 US 201715843173 A US201715843173 A US 201715843173A US 2018186871 A1 US2018186871 A1 US 2018186871A1
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seq
cdr1
cdr2
cdr3
variable region
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Stuart Alexander Cook
Sebastian Schaefer
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National University of Singapore
Singapore Health Services Pte Ltd
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National University of Singapore
Singapore Health Services Pte Ltd
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Priority claimed from GBGB1621446.2A external-priority patent/GB201621446D0/en
Priority claimed from GBGB1709535.7A external-priority patent/GB201709535D0/en
Application filed by National University of Singapore, Singapore Health Services Pte Ltd filed Critical National University of Singapore
Publication of US20180186871A1 publication Critical patent/US20180186871A1/en
Assigned to NATIONAL UNIVERSITY OF SINGAPORE, SINGAPORE HEALTH SERVICES PTE LTD reassignment NATIONAL UNIVERSITY OF SINGAPORE ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: SCHAEFER, SEBASTIAN
Assigned to NATIONAL UNIVERSITY OF SINGAPORE, SINGAPORE HEALTH SERVICES PTE LTD reassignment NATIONAL UNIVERSITY OF SINGAPORE ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: COOK, Stuart Alexander
Priority to US16/726,173 priority Critical patent/US11078268B2/en
Abandoned legal-status Critical Current

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    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/244—Interleukins [IL]
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    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
    • A61K47/6835—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment the modifying agent being an antibody or an immunoglobulin bearing at least one antigen-binding site
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    • C07K14/52—Cytokines; Lymphokines; Interferons
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    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C07K14/7155—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for interleukins [IL]
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
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    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
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    • C07K2317/00—Immunoglobulins specific features
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    • C07K2317/515—Complete light chain, i.e. VL + CL
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    • C07—ORGANIC CHEMISTRY
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    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
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    • C07—ORGANIC CHEMISTRY
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    • C07K2317/00—Immunoglobulins specific features
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    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565—Complementarity determining region [CDR]
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    • C07K2317/622—Single chain antibody (scFv)
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    • C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
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    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
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    • G01N2333/52—Assays involving cytokines
    • G01N2333/54—Interleukins [IL]
    • G01N2333/5431—IL-11
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis

Definitions

  • the present invention relates to antibodies that bind to interleukin 11 (IL-11).
  • IL-11 interleukin 11
  • Fibrotic disorders include both rare, genetically-driven diseases such as scleroderma, idiopathic pulmonary fibrosis and hypertrophic cardiomyopathy, dilated cardiomyopathy (DCM), and common diseases like atrial fibrillation, ventricular fibrillation, non-alcoholic fatty liver disease and diabetic kidney disease. Due to the significant impact on world-wide morbidity and mortality, there is a need to develop therapeutics to inhibit the fibrotic response (Nanthakumar et al., 2015 Nat Rev Drug Discov 14, 693-720).
  • fibrosis A major hallmark of fibrosis is the pathologic activation of resident fibroblasts that drives their transition from a quiescent state to proliferating, secretory and contractile myofibroblasts (Hinz et al., 2010 Am J Pathology 170, 1807-1816). Stimuli such as mechanical stress and pro-fibrotic cytokines can activate fibroblasts.
  • Stimuli such as mechanical stress and pro-fibrotic cytokines can activate fibroblasts.
  • the TGF ⁇ 1 pathway is considered to be of central importance for the fibrotic response (Leask and Abraham, 2004 The FASEB Journal 18, 816-827) and its inhibition is a therapeutic strategy that is under investigation (Gourdie et al., 2016 Nature Reviews Drug Discovery 15, 620-638).
  • direct inhibition of multi-functional TGF ⁇ 1 is associated with severe side effects such as inflammation and cancer susceptibility.
  • the present invention provides an antibody or antigen binding fragment, optionally isolated, which is capable of binding to IL-11, wherein the antibody or antigen binding fragment is a fully human antibody or antigen binding fragment and is capable of inhibiting IL-11 trans signalling.
  • the present invention provides an antibody or antigen binding fragment, optionally isolated, which is capable of binding to IL-11, comprising the amino acid sequences i) to vi):
  • LC-CDR1 (SEQ ID NO: 239) X 1 X 2 DX 3 GX 4 YX 5 Y; (SEQ ID NO: 240) X 6 SNX 7 GX 8 X 9 X 10 ; (SEQ ID NO: 241) QX 11 X 12 SSX 13 ; (SEQ ID NO: 242) X 14 GX 15 IASNX 16 ; (SEQ ID NO: 101) QDVGRY; or (SEQ ID NO: 161) SLRGYY; ii) LC-CDR2: (SEQ ID NO: 243) DVX 17 ; (SEQ ID NO: 244) X 18 NX 19 ; (SEQ ID NO: 245) X 20 AS; (SEQ ID NO: 246) X 21 DX 22 ; (SEQ ID NO: 247) EVX 23 ; or (SEQ ID NO: 248) DX 24 X 25 ; iii) LC-CDR3: (SEQ ID NO: 249)
  • X 1 S or I
  • X 2 S or R
  • X 3 V or I
  • X 4 G
  • a or N
  • X 5 N, E, K or D
  • X 6 S or Y
  • X 7 I or V
  • X 8 S, N or Y
  • X 9 N, Y or D
  • X 10 L, T or A
  • X 11 G, S or I
  • X 12 S, I or V
  • X 13 Y or N
  • X 14 S or T
  • X 15 S or N
  • X 16 Y or R
  • X 17 S, T or G
  • X 18 R
  • X 19 N or D
  • X 20 A or G
  • X 21 E, D or N
  • X 22 N or D
  • X 23 S, F or N
  • X 24 N or V
  • X 25 H or T
  • X 26 S, N or G
  • X 27 S or T
  • X 28 S or G
  • HC-CDR1 is one of VSSNSAAWN (SEQ ID NO:180), GFTFSGAY (SEQ ID NO:183), GFTFSSYG (SEQ ID NO:186), GFTFSSYA (SEQ ID NO:190), GFSFRSYG (SEQ ID NO:193), GFTFRSYG (SEQ ID NO:196), GFSFSSYA (SEQ ID NO:212), WIFLKSYA (SEQ ID NO:204), GFSLNSYG (SEQ ID NO:217), GGTFSSYA (SEQ ID NO:209), GGSISSSNW (SEQ ID NO:220), GFSLSSYG (SEQ ID NO:201), GGTFSSYA (SEQ ID NO:209), ILPSDSYA (SEQ ID NO:226), GYTFTSYG (SEQ ID NO:228), GFTFGSYG (SEQ ID NO:234) or GFSLGSYG (SEQ ID NO:238).
  • VSSNSAAWN S
  • HC-CDR2 is one of YRSKWYN (SEQ ID NO:181), ISYDGSNK (SEQ ID NO:184), ISYDGSDK (SEQ ID NO:188), IIPIFGTA (SEQ ID NO:210), IYHSGST (SEQ ID NO:221), or ISAYNGNT (SEQ ID NO:229).
  • HC-CDR3 is one of ARGTRGYFDY (SEQ ID NO:182), ARDLYAFDI (SEQ ID NO:185), AKIGATDPLDY (SEQ ID NO:187), AKDLSGLPIIDY (SEQ ID NO:189), ARRGYFDY (SEQ ID NO:191), ARIAAADGMDV (SEQ ID NO:192), ARITHDYGDFSDAFDI (SEQ ID NO:194), AKLYSGSSNFDY (SEQ ID NO:195), AKLSGPNGVDY (SEQ ID NO:197), ARGQNVDL (SEQ ID NO:198), ARIMGYDYGDYDVVDY (SEQ ID NO:199), ARRGYGDY (SEQ ID NO:213), ARVGFSSWYPDLYYFDY (SEQ ID NO:205), AKFARGVYLFDY (SEQ ID NO:215), ARVQSGEPESDY (SEQ ID NO:216), ARMVNLYYGDAFDI (SEQ ID NO
  • LC-CDR1 is one of QDVGRY (SEQ ID NO:101), TGNIASNR (SEQ ID NO:104), SSDVGGYNY (SEQ ID NO:107), SSDVGAYNY (SEQ ID NO:110), SSDIGAYNY (SEQ ID NO:114), SSNIGSNY (SEQ ID NO:116), ISDVGGYNY (SEQ ID NO:122), SSNIGNNL (SEQ ID NO:126), SSDVGGYDY (SEQ ID NO:128), QSVSSN (SEQ ID NO:137), SSNIGNNY (SEQ ID NO:140), YSNVGSNL (SEQ ID NO:144), SSNIGSNT (SEQ ID NO:147), SRDVGGYNY (SEQ ID NO:150), SGSIASNY (SEQ ID NO:152), QIISSY (SEQ ID NO:155), SSDVGGYEY (SEQ ID NO:159), SLRGYY (SEQ ID NO:161), SSNIGSYY (SEQ ID NO
  • LC-CDR2 is one of AAS (SEQ ID NO:102), DNH (SEQ ID NO:105), DVS (SEQ ID NO:108), EVS (SEQ ID NO:111), RNN (SEQ ID NO:117), DVT (SEQ ID NO:123), DVH (SEQ ID NO:129), DVG (SEQ ID NO:133), EVN (SEQ ID NO:135), DVT (SEQ ID NO:123), GAS (SEQ ID NO:138), DNT (SEQ ID NO:141), EDD (SEQ ID NO:145), INN (SEQ ID NO:148), DDN (SEQ ID NO:153), EDN (SEQ ID NO:157), GNN (SEQ ID NO:162), RND (SEQ ID NO:165), EVF (SEQ ID NO:168) or NDN (SEQ ID NO:171).
  • AAS SEQ ID NO:102
  • DNH SEQ ID NO:105
  • DVS SEQ ID NO:108
  • EVS SEQ ID NO:111
  • LC-CDR3 is one of QQYRSAPLA (SEQ ID NO:103), QSYDYSSVI (SEQ ID NO:106), SSYTSSSSWV (SEQ ID NO:109), SSYTSSNTLV (SEQ ID NO:112), SSYTSSSTVV (SEQ ID NO:113), SSYTTSSTVV (SEQ ID NO:115), AAWDGSLSGWV (SEQ ID NO:118), SSYTSSSTWV (SEQ ID NO:119), CSYAGSYTFV (SEQ ID NO:120), NSYTSSTPYV (SEQ ID NO:121), SSYAGSYTWV (SEQ ID NO:124), GSYTSSNTQV (SEQ ID NO:125), AAWDDSLSAGV (SEQ ID NO:127), SSYTSSITWV (SEQ ID NO:130), CSYAGSYTWV (SEQ ID NO:131), GSYTSSSTWV (SEQ ID NO:103),
  • the antibody or antigen binding fragment has at least one heavy chain variable region incorporating the following CDRs:
  • HC-CDR1 VSSNSAAWN (SEQ ID NO: 181) HC-CDR2: YRSKWYN (SEQ ID NO: 182) HC-CDR3: ARGTRGYFDY; or (SEQ ID NO: 183) HC-CDR1: GFTFSGAY (SEQ ID NO: 184) HC-CDR2: ISYDGSNK (SEQ ID NO: 185) HC-CDR3: ARDLYAFDI; or (SEQ ID NO: 186) HC-CDR1: GFTFSSYG (SEQ ID NO: 184) HC-CDR2: ISYDGSNK (SEQ ID NO: 187) HC-CDR3: AKIGATDPLDY; or (SEQ ID NO: 186) HC-CDR1: GFTFSSYG (SEQ ID NO: 188) HC-CDR2: ISYDGSDK (SEQ ID NO: 189) HC-CDR3: AKDLSGLPIIDY
  • the antibody or antigen binding fragment has at least one light chain variable region incorporating the following CDRs:
  • the present invention provides an antibody or antigen binding fragment, optionally isolated, which is capable of binding to IL-11, having at least one heavy chain variable region incorporating the following CDRs:
  • HC-CDR1 VSSNSAAWN (SEQ ID NO: 181) HC-CDR2: YRSKWYN (SEQ ID NO: 182) HC-CDR3: ARGTRGYFDY; or (SEQ ID NO: 183) HC-CDR1: GFTFSGAY (SEQ ID NO: 184) HC-CDR2: ISYDGSNK (SEQ ID NO: 185) HC-CDR3: ARDLYAFDI; or (SEQ ID NO: 186) HC-CDR1: GFTFSSYG (SEQ ID NO: 184) HC-CDR2: ISYDGSNK (SEQ ID NO: 187) HC-CDR3: AKIGATDPLDY; or (SEQ ID NO: 186) HC-CDR1: GFTFSSYG (SEQ ID NO: 188) HC-CDR2: ISYDGSDK (SEQ ID NO: 189) HC-CDR3: AKDLSGLPIIDY
  • the present invention provides an antibody or antigen binding fragment, optionally isolated, which is capable of binding to IL-11, comprising a light chain and a heavy chain variable region sequence, wherein:
  • the present invention provides an antibody or antigen binding fragment, optionally isolated, which is capable of binding to IL-11, comprising a light chain and a heavy chain variable region sequence, wherein:
  • the present invention provides an antibody or antigen binding fragment, optionally isolated, which is capable of binding to IL-11, which is capable of inhibiting IL-11 trans signalling, optionally wherein the antibody or antigen binding fragment is an antibody or antigen binding fragment according to the present invention.
  • the antibody or antigen binding fragment is conjugated to a drug moiety or a detectable moiety.
  • the present invention provides a complex, optionally an in vitro complex and/or optionally isolated, comprising an antibody or antigen binding fragment according to the present invention bound to IL-11.
  • the present invention provides a composition comprising the antibody or antigen binding fragment according to the present invention, and at least one pharmaceutically-acceptable carrier.
  • the present invention provides an isolated nucleic acid encoding the antibody or antigen binding fragment according to the present invention.
  • the nucleic acid comprises a sequence having at least 60%, 70%, 80%, 90%, 95%, or greater sequence identity to one of SEQ ID NOs:476 to 539, or an equivalent sequence as a result of codon degeneracy. In some embodiments, the nucleic acid comprises a sequence having at least 60%, 70%, 80%, 90%, 95%, or greater sequence identity to one of SEQ ID NOs:571 to 580, or an equivalent sequence as a result of codon degeneracy.
  • the present invention provides a vector comprising the nucleic acid according to the present invention.
  • the present invention provides a host cell comprising the vector according to the present invention.
  • the present invention provides a method for making an antibody or antigen binding fragment according to the present invention, comprising culturing the host cell according to the present invention under conditions suitable for the expression of the antibody or antigen binding fragment, and recovering the antibody or antigen binding fragment.
  • the present invention provides an antibody, antigen binding fragment, or composition according to the present invention for use in therapy, or in a method of medical treatment.
  • the present invention provides an antibody, antigen binding fragment, or composition according to the present invention for use in the treatment or prevention of fibrosis, or a disease/disorder characterised by fibrosis.
  • the present invention provides an antibody, antigen binding fragment, or composition according to the present invention for use in the treatment of a cancer.
  • the present invention provides the use of an antibody, antigen binding fragment, or composition according to the present invention in the manufacture of a medicament for use in the treatment or prevention of fibrosis or a disease/disorder characterised by fibrosis.
  • the present invention provides the use of an antibody, antigen binding fragment, or composition according to the present invention in the manufacture of a medicament for use in the treatment or prevention of a cancer.
  • the present invention provides a method of treating fibrosis comprising administering an antibody, antigen binding fragment, or composition according to the present invention to a subject suffering from fibrosis or a disease/disorder characterised by fibrosis.
  • the present invention provides a method of treating cancer comprising administering an antibody, antigen binding fragment, or composition according to the present invention to a subject suffering from a cancer.
  • the present invention provides an antibody or antigen binding fragment for use in a method of treating a disease in which IL-11 mediated signalling is implicated in the pathology of the disease, wherein the antibody or antigen binding fragment is capable of inhibiting IL-11 trans signalling.
  • the present invention provides the use of an antibody or antigen binding fragment in the manufacture of a medicament for use in the treatment of a disease in which IL-11 mediated signalling is implicated in the pathology of the disease, wherein the antibody or antigen binding fragment is capable of inhibiting IL-11 trans signalling.
  • the present invention provides a method of treating a disease in which IL-11 mediated signalling is implicated in the pathology of the disease, comprising administering an antibody or antigen binding fragment to a subject suffering from the disease, wherein the antibody or antigen binding fragment is capable of inhibiting IL-11 trans signalling.
  • the present invention provides a method comprising contacting a sample containing, or suspected to contain, IL-11 with an antibody or antigen binding fragment according to the present invention and detecting the formation of a complex of the antibody or antigen binding fragment with IL-11.
  • the present invention provides a method of diagnosing a disease or condition in a subject, the method comprising contacting, in vitro, a sample from the subject with an antibody or antigen binding fragment according to the present invention and detecting the formation of a complex of the antibody or antigen binding fragment with IL-11.
  • the present invention provides a method of selecting or stratifying a subject for treatment with an IL-11-targeted agent, the method comprising contacting, in vitro, a sample from the subject with the antibody or antigen binding fragment according to the present invention and detecting the formation of a complex of the antibody or antigen binding fragment with IL-11.
  • the present invention provides the use of an antibody or antigen binding fragment according to the present invention for the detection of IL-11 in vitro or in vivo.
  • the present invention provides the use of an antibody or antigen binding fragment according to the present invention as an in vitro or in vivo diagnostic or prognostic agent.
  • the present invention provides an antibody or antigen binding fragment, optionally isolated, which is capable of binding to IL-11, comprising the amino acid sequences i) to vi):
  • LC-CDR1 (SEQ ID NO: 393) X 138 X 139 DVGGYX 140 X 141 , (SEQ ID NO: 394) SSDVX 142 X 143 YX 144 Y, (SEQ ID NO: 395) X 145 X 146 DX 147 GAYNY, (SEQ ID NO: 396) SSDIGX 148 YNY, (SEQ ID NO: 397) X 149 SDVGAYDY, (SEQ ID NO: 398) SGDVGTYX 150 Y, (SEQ ID NO: 399) QX 151 IX 152 SY, (SEQ ID NO: 400) QSX 153 SSSY, (SEQ ID NO: 401) RX 154 DX 155 GGYDX 156 , (SEQ ID NO: 284) SSNVGGYNY, (SEQ ID NO: 349) GSNVGGYNY or (SEQ ID NO: 359) QSVNSAY; ii) LC-CDR2: (SEQ ID NO
  • HC-CDR1 is one of GFTFSSYG (SEQ ID NO:186), GFTFSSYA (SEQ ID NO:190) or GFTFGSYG (SEQ ID NO:234).
  • HC-CDR2 is one of ISYDGSNK (SEQ ID NO:184) or ISYDGSNR (SEQ ID NO:381).
  • HC-CDR3 is one of AKIGATDPLDY (SEQ ID NO:187), ARIMGYDYGDYDVVDY (SEQ ID NO:199), AKGGKSYYGFDY (SEQ ID NO:207), AKGSYYFDY (SEQ ID NO:235) or AKLSGPNGVDY (SEQ ID NO:197).
  • LC-CDR1 is one of SSDVGGYNF (SEQ ID NO:294), SSDVGGYEY (SEQ ID NO:159), SRDVGGYNY (SEQ ID NO:150), NSDVGGYNY (SEQ ID NO:300), SSDVGGYDY (SEQ ID NO:128), SSDVGGYNY (SEQ ID NO:107), ISDVGGYNY (SEQ ID NO:122), SSDVGDYDY (SEQ ID NO:317), SSDVAGYNY (SEQ ID NO:330), SSDVGTYNY (SEQ ID NO:344), NTDVGAYNY (SEQ ID NO:272), SNDIGAYNY (SEQ ID NO:306), SSDVGAYNY (SEQ ID NO:110), SSDIGVYNY (SEQ ID NO:347), SSDIGGYNY (SEQ ID NO:326), SSDVGAYDY (SEQ ID NO:333), GSDVGAYDY (SEQ ID NO:322), SGDVGTYNY (SEQ ID NO:298)
  • LC-CDR2 is one of DVS (SEQ ID NO:108), DVV (SEQ ID NO:275), DVT (SEQ ID NO:123), DVD (SEQ ID NO:295), DVN (SEQ ID NO:291), DVG (SEQ ID NO:133), AAS (SEQ ID NO:102) or GAS (SEQ ID NO:138).
  • LC-CDR3 is one of CSYAGSYTWV (SEQ ID NO:131), SSYAGSYTWV (SEQ ID NO:124), CSYAGSYSWV (SEQ ID NO:273), CSYAGGYTWV (SEQ ID NO:276), NSYAGSYTWV (SEQ ID NO:278), CSYAGSYVWV (SEQ ID NO:285), CSYAGRYTWI (SEQ ID NO:296), CSYAGRYTWM (SEQ ID NO:336), CSYAGTYTWV (SEQ ID NO:340), CSYAGFYTWV (SEQ ID NO:345), CSYAGSHIWV (SEQ ID NO:308), CSYAGRYTWV (SEQ ID NO:313), CSYAGNYTWM (SEQ ID NO:315), CSYAGSYTWI (SEQ ID NO:324), ASYAGNYNWV (SEQ ID NO:
  • HC-CDR1 (SEQ ID NO: 186) GFTFSSYG HC-CDR2: (SEQ ID NO: 184) ISYDGSNK HC-CDR3: (SEQ ID NO: 187) AKIGATDPLDY; or HC-CDR1: (SEQ ID NO: 190) GFTFSSYA HC-CDR2: (SEQ ID NO: 184) ISYDGSNK HC-CDR3: (SEQ ID NO: 199) ARIMGYDYGDYDVVDY; or HC-CDR1: (SEQ ID NO: 234) GFTFGSYG HC-CDR2: (SEQ ID NO: 184) ISYDGSNK HC-CDR3: (SEQ ID NO: 187) AKIGATDPLDY; or HC-CDR1: (SEQ ID NO: 186) GFTFSSYG HC-CDR2: (SEQ ID NO: 381) ISYDGSNR HC-CDR3: (SEQ ID NO: 187) AKI
  • LC-CDR1 (SEQ ID NO: 110) SSDVGAYNY LC-CDR2: (SEQ ID NO: 108) DVS LC-CDR3: (SEQ ID NO: 268) SSFTTSIAWV; or LC-CDR1: (SEQ ID NO: 107) SSDVGGYNY LC-CDR2: (SEQ ID NO: 108) DVS LC-CDR3: (SEQ ID NO: 131) CSYAGSYTWV; or LC-CDR1: (SEQ ID NO: 107) SSDVGGYNY LC-CDR2: (SEQ ID NO: 108) DVS LC-CDR3: (SEQ ID NO: 124) SSYAGSYTWV; or LC-CDR1: (SEQ ID NO: 272) NTDVGAYNY LC-CDR2: (SEQ ID NO: 108) DVS LC-CDR3: (SEQ ID NO: 273) CSYAGSYSWV; or LC-CDR1: (SEQ ID
  • the present invention provides an antibody or antigen binding fragment, optionally isolated, which is capable of binding to IL-11, comprising a light chain and a heavy chain variable region sequence, wherein:
  • the present invention provides an antibody or antigen binding fragment, optionally isolated, which is capable of binding to IL-11, comprising a light chain and a heavy chain variable region sequence, wherein:
  • the present invention provides an antibody or antigen binding fragment, optionally isolated, which is capable of binding to IL-11, comprising an amino acid sequence having at least 85% sequence identity to the sequence of one of SEQ ID NOs: 412 to 475.
  • the present invention provides an antibody or antigen binding fragment, optionally isolated, which is capable of binding to IL-11, comprising the amino acid sequences i) to vi):
  • LC-CDR1 (SEQ ID NO: 555) ENVVTY, (SEQ ID NO: 558) QSIGTS, (SEQ ID NO: 562) QSLLYNSSQKNY or (SEQ ID NO: 565) QDVGTA; ii) LC-CDR2: (SEQ ID NO: 569) X 184 AS; iii) LC-CDR3: (SEQ ID NO: 570) X 185 QX 186 X 187 SX 188 X 189 X 190 T; iv) HC-CDR1: (SEQ ID NO: 567) GYTFTX 180 YX 181 ; v) HC-CDR2: (SEQ ID NO: 568) INPX 182 NGGX 183 or (SEQ ID NO: 552) IYPRSSNT; vi) HC-CDR3: (SEQ ID NO: 544) ARGELGHWYFDV, (SEQ ID NO: 547) AREGPYGYT
  • HC-CDR1 is one of GYTFTDYN (SEQ ID NO:542) or GYTFTSYG (SEQ ID NO:228).
  • HC-CDR2 is one of INPHNGGP (SEQ ID NO:543), INPDNGGT (SEQ ID NO:546), INPNNGGI (SEQ ID NO:549) or IYPRSSNT (SEQ ID NO:552).
  • HC-CDR3 is one of ARGELGHWYFDV (SEQ ID NO:544), AREGPYGYTWFAY (SEQ ID NO:547), ARNPSLYDGYLDC (SEQ ID NO:550) or ARANWVGYFDV (SEQ ID NO:553).
  • LC-CDR1 is one of ENVVTY (SEQ ID NO:555), QSIGTS (SEQ ID NO:558), QSLLYNSSQKNY (SEQ ID NO:562) or QDVGTA (SEQ ID NO:565).
  • LC-CDR2 is one of GAS (SEQ ID NO:138), YAS (SEQ ID NO:559) or WAS (SEQ ID NO:563).
  • LC-CDR3 is one of GQGYSYPYT (SEQ ID NO:556), QQSNSWPLT (SEQ ID NO:560), QQYYSYPLT (SEQ ID NO:563) or QQYSSYRT (SEQ ID NO:566).
  • HC-CDR1 (SEQ ID NO: 542) GYTFTDYN HC-CDR2: (SEQ ID NO: 543) INPHNGGP HC-CDR3: (SEQ ID NO: 544) ARGELGHWYFDV; or HC-CDR1: (SEQ ID NO: 542) GYTFTDYN HC-CDR2: (SEQ ID NO: 546) INPDNGGT HC-CDR3: (SEQ ID NO: 547) AREGPYGYTWFAY; or HC-CDR1: (SEQ ID NO: 542) GYTFTDYN HC-CDR2: (SEQ ID NO: 549) INPNNGGI HC-CDR3: (SEQ ID NO: 550) ARNPSLYDGYLDC; or HC-CDR1: (SEQ ID NO: 228) GYTFTSYG HC-CDR2: (SEQ ID NO: 552) IYPRSSNT HC-CDR3: (SEQ ID NO: 553) A
  • LC-CDR1 (SEQ ID NO: 555) ENVVTY LC-CDR2: (SEQ ID NO: 138) GAS LC-CDR3: (SEQ ID NO: 556) GQGYSYPYT; or LC-CDR1: (SEQ ID NO: 558) QSIGTS LC-CDR2: (SEQ ID NO: 559) YAS LC-CDR3: (SEQ ID NO: 560) QQSNSWPLT; or LC-CDR1: (SEQ ID NO: 562) QSLLYNSSQKNY LC-CDR2: (SEQ ID NO: 563) WAS LC-CDR3: (SEQ ID NO: 581) QQYYSYPLT; or LC-CDR1: (SEQ ID NO: 565) QDVGTA LC-CDR2: (SEQ ID NO: 563) WAS LC-CDR3: (SEQ ID NO: 566) QQYSSYRT.
  • the present invention provides an antibody or antigen binding fragment, optionally isolated, which is capable of binding to IL-11, comprising a light chain and a heavy chain variable region sequence, wherein:
  • the present invention provides an antibody or antigen binding fragment, optionally isolated, which is capable of binding to IL-11, comprising a light chain and a heavy chain variable region sequence, wherein:
  • the present invention relates to antibodies with specificity for interleukin-11 (IL-11).
  • IL-11 interleukin-11
  • the present disclosure describes the identification of IL-11/IL-11R signalling as a key mediator of fibrosis, and the generation and functional characterisation of anti-IL-11 antibodies. Therapeutic and diagnostic uses of the antibodies is also described.
  • Interleukin 11 also known as adipogenesis inhibitory factor
  • IL-11 is a pleiotropic cytokine and a member of the IL-6 family of cytokines that includes IL-6, IL-11, IL-27, IL-31, oncostatin M (OSM), leukemia inhibitory factor (LIF), cardiotrophin-1 (CT-1), cardiotrophin-like cytokine (CLC), ciliary neurotrophic factor (CNTF) and neuropoetin (NP-1).
  • OSM oncostatin M
  • LIF leukemia inhibitory factor
  • CT-1 cardiotrophin-1
  • CLC cardiotrophin-like cytokine
  • CNTF ciliary neurotrophic factor
  • NP-1 neuropoetin
  • IL-11 is transcribed with a canonical signal peptide that ensures efficient secretion from cells.
  • the immature form of human IL-11 is a 199 amino acid polypeptide whereas the mature form of IL-11 encodes a protein of 178 amino acid residues (Garbers and Scheller., Biol. Chem. 2013; 394(9):1145-1161).
  • the human IL-11 amino acid sequence is available under UniProt accession no. P20809 (P20809.1 GI:124294).
  • Recombinant human IL-11 (oprelvekin) is also commercially available.
  • IL-11 refers to an IL-11 from any species and includes isoforms, fragments, variants or homologues of an IL-11 from any species.
  • the IL-11 is mammalian IL-11 (e.g. cynomolgous, human and/or rodent (e.g. rat and/or murine) IL-11).
  • Isoforms, fragments, variants or homologues of an IL-11 may optionally be characterised as having at least 70%, preferably one of 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% amino acid sequence identity to the amino acid sequence of immature or mature IL-11 from a given species, e.g. human.
  • Isoforms, fragments, variants or homologues of an IL-11 may optionally be characterised by ability to bind IL-11R ⁇ (preferably from the same species) and stimulate signal transduction in cells expressing IL-11R ⁇ and gp130 (e.g. as described in Curtis et al. Blood, 1997, 90(11); or Karpovich et al. Mol. Hum. Reprod. 2003 9(2): 75-80).
  • a fragment of IL-11 may be of any length (by number of amino acids), although may optionally be at least 25% of the length of mature IL-11 and may have a maximum length of one of 50%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% of the length of mature IL-11.
  • a fragment of IL-11 may have a minimum length of 10 amino acids, and a maximum length of one of 15, 20, 25, 30, 40, 50, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190 or 195 amino acids.
  • Gp130 is a transmembrane protein that forms one subunit of the type I cytokine receptor with the IL-6 receptor family. Specificity is gained through an individual IL-11 ⁇ -receptor (IL-11R ⁇ ), which does not directly participate in signal transduction, although the initial cytokine binding event to the ⁇ -receptor leads to the final complex formation with the 6-receptors.
  • IL-11 activates a downstream signalling pathway, which is predominantly the mitogen-activated protein kinase (MAPK)-cascade and the Janus kinase/signal transducer and activator of transcription (Jak/STAT) pathway (Garbers and Scheller, supra).
  • MAPK mitogen-activated protein kinase
  • Jak/STAT Janus kinase/signal transducer and activator of transcription pathway
  • Human IL-11R ⁇ is a 422 amino acid polypeptide (Genbank accession no. NP 001136256.1 GI:218505839) and shares ⁇ 85% nucleotide and amino acid sequence identity with the murine IL-11R ⁇ (Du and Williams., Blood Vol, 89, No, 11, Jun. 1, 1997). Two isoforms of IL-11R ⁇ have been reported, which differ in the cytoplasmic domain (Du and Williams, supra). In some embodiments as used herein, the IL-11R ⁇ may be IL-11R ⁇ isoform 1 or IL-11R ⁇ isoform 2.
  • the IL-11 receptor ⁇ -chain shares many structural and functional similarities with the IL-6 receptor ⁇ -chain (IL-6R ⁇ ).
  • the extracellular domain shows 24% amino acid identity including the characteristic conserved Trp-Ser-X-Trp-Ser (WSXWS) motif.
  • the short cytoplasmic domain (34 amino acids) lacks the Box 1 and 2 regions that are required for activation of the JAK/STAT signalling pathway.
  • IL-11R ⁇ binds its ligand with a low affinity (Kd ⁇ 10 nmol/L) and alone is insufficient to transduce a biological signal.
  • Kd ⁇ 400 to 800 pmol/L a high affinity receptor capable of signal transduction requires co-expression of the IL-11R ⁇ and gp130 (Curtis et al (Blood 1997 Dec. 1; 90 (11):4403-12; Hilton et al., EMBO J 13:4765, 1994; Nandurkar et al., Oncogene 12:585, 1996). Binding of IL-11 to cell-surface IL-11R ⁇ induces heterodimerization, tyrosine phosphorylation, activation of gp130 and MAPK and/or Jak/STAT signalling as described above.
  • the receptor binding sites on murine IL-11 have been mapped and three sites—I, II and III—identified. Binding to gp130 is reduced by substitutions in the site II region and by substitutions in the site III region. Site III mutants show no detectable agonist activity and have IL-11R ⁇ antagonist activity (Cytokine Inhibitors Chapter 8; edited by Gennaro Ciliberto and Rocco Savino, Marcel Dekker, Inc. 2001).
  • a soluble IL-11R ⁇ can also form biologically active soluble complexes with IL-11 (Planet al., 1999 FEBS Lett, 450, 117-122) raising the possibility that, similar to IL-6, IL-11 may in some instances bind soluble IL-11R ⁇ prior to binding cell-surface gp130 (Garbers and Scheller, supra).
  • Curtis et al (Blood 1997 Dec. 1; 90 (11):4403-12) describe expression of a soluble murine IL-11 receptor alpha chain (sIL-11R ⁇ ) and examined signalling in cells expressing gp130.
  • sIL-11R mediated IL-11 dependent differentiation of M1 leukemic cells and proliferation in Ba/F3 cells and early intracellular events including phosphorylation of gp130, STATS and SHP2 similar to signalling through transmembrane IL-11R.
  • IL-11 trans signalling may be a very important component of IL-11 mediated signalling, and may even be the most common form of IL-11 mediated signalling, because whilst the expression of IL-11R ⁇ is restricted to a relatively small subset of cell types, gp130 is expressed on a wide range of cell types.
  • IL-11 trans signalling is used to refer to signalling which is triggered by binding of IL-11 bound to IL-11R ⁇ , to gp130.
  • the IL-11 may be bound to IL-11R ⁇ as a non-covalent complex.
  • the gp130 is membrane-bound and expressed by the cell in which signalling occurs following binding of the IL-11:IL-11Ra complex to gp130.
  • the IL-11R ⁇ may be a soluble IL-11R ⁇ .
  • the soluble IL-11R ⁇ is a soluble (secreted) isoform of IL-11R ⁇ (e.g. lacking a transmembrane domain).
  • the soluble IL-11R ⁇ is the liberated product of proteolytic cleavage of the extracellular domain of cell membrane bound IL-11R ⁇ .
  • the IL-11R ⁇ may be cell membrane-bound, and signalling through gp130 may be triggered by binding of IL-11 bound to cell-membrane-bound IL-11R ⁇ .
  • an IL-11 receptor refers to a polypeptide capable of binding IL-11 and inducing signal transduction in cells expressing gp130.
  • An IL-11 receptor may be from any species and includes isoforms, fragments, variants or homologues of an IL-11 receptor from any species. In preferred embodiments the species is human ( Homo sapiens ). In some embodiments the IL-11 receptor may be IL-11Ra.
  • Isoforms, fragments, variants or homologues of an IL-11R ⁇ may optionally be characterised as having at least 70%, preferably one of 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% amino acid sequence identity to the amino acid sequence of IL-11R ⁇ from a given species, e.g. human.
  • Isoforms, fragments, variants or homologues of an IL-11R ⁇ may optionally be characterised by ability to bind IL-11 (preferably from the same species) and stimulate signal transduction in cells expressing IL-11R ⁇ and gp130 (e.g. as described in Curtis et al.
  • a fragment of an IL-11 receptor may be of any length (by number of amino acids), although may optionally be at least 25% of the length of the mature IL-11R ⁇ and have a maximum length of one of 50%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% of the length of the mature IL-11R ⁇ .
  • a fragment of an IL-11 receptor fragment may have a minimum length of 10 amino acids, and a maximum length of one of 15, 20, 25, 30, 40, 50, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 250, 300, 400, or 415 amino acids.
  • TGF ⁇ 1 has been shown to induce IL-11 expression in fibroblasts (Elias et al., 1994 J. Immunol. 152, 2421-2429).
  • IL-11 has been proposed to function mainly as a thrombopoietic growth factor, which underpinned the use of recombinant IL-11 (Neumega (Oprelvekin)) as a therapeutic agent to increase platelet count.
  • IL-11 The role of IL-11 in fibrosis is not clear. The majority of studies suggest an anti-fibrotic function for IL-11 in the heart (Obana et al., 2010 Circulation 121, 684-691; Obana et al., 2012 Heart and Circulatory Physiology 303, H569-77) and kidney (Ham et al., 2013 Anesthesiology 119, 1389-1401; Stangou et al., 2011 J. Nephrol. 24, 106-111). Kurahara et al., J. Smooth Muscle Res. 2016; 52: 78-92 describes IL-11 as an anti-fibrotic cytokine, and suggests that IL-11 supresses ⁇ SMA expression.
  • IL-11 has also been suggested to be an anti-inflammatory factor in several tissues and chronic inflammatory diseases (Trepicchio and Dorner, 1998 Expert Opin Investig Drugs 7, 1501-1504; Zhu et al., 2015 PLoS ONE 10, e0126296). These studies suggest that the observed secretion of IL-11 in response to TGF ⁇ 1 is a protective mechanism.
  • IL-11 signalling may be involved in pathology of diseases of the lung. Inhibition of IL-11 either via antibodies or a mutated recombinant IL-11 in a model of tuberculosis revealed a positive feedback loop in vivo and diminished histopathology of the lung (Kapina et al., 2011 PLoS ONE 6, e21878; Shepelkova et al., 2016 Journal of Infectious Diseases 214, jiw176), fibrosis of the murine airway has been associated with IL-11 expression (Tang et al., 1996 The Journal of Clinical Investigation 98, 2845-2853). When the pro-fibrotic function of IL-13 in lung tissue was investigated in IL-11 RA ⁇ / ⁇ mice, IL-11 signalling was implicated in the mechanism (Chen et al., 2005 J. Immunol. 174, 2305-2313).
  • IL-11 was also found to be elevated in the airway of patients with severe asthma (Minshall et al., 2000 Respiratory Research 14, 1-14), is overexpressed in the lungs of IPF patients (Lindahl et al., 2013 Respiratory Research 14, 1-14) and is elevated in skin lesions in atopic dermatitis patients (Toda et al., 2003 J Allergy Clin Immun 111, 875-881). It is uncertain whether these associations are due to increased IL-11 gene/protein expression as a response to disease processes, or whether IL-11 is an effector of disease processes.
  • Antibodies and antigen-binding fragments according to the present invention bind to IL-11 (interleukin 11). In some embodiments, the antibody/fragment binds to human IL-11. In some embodiments, the antibody/fragment binds to non-human primate IL-11. In some embodiments, the antibody/fragment binds to murine IL-11.
  • antibody we include fragments and derivatives thereof, or a synthetic antibody or synthetic antibody fragment.
  • an antibody is a polypeptide capable of binding specifically to the relevant target molecule (i.e. the antigen for which the antibody is specific).
  • Antibodies according to the present invention may be provided in isolated form.
  • antibodies can be prepared to most antigens.
  • the antigen-binding portion may be a part of an antibody (for example a Fab fragment) or a synthetic antibody fragment (for example a single chain Fv fragment [ScFv]).
  • Suitable monoclonal antibodies to selected antigens may be prepared by known techniques, for example those disclosed in “Monoclonal Antibodies: A manual of techniques”, H Zola (CRC Press, 1988) and in “Monoclonal Hybridoma Antibodies: Techniques and Applications”, J G R Hurrell (CRC Press, 1982). Chimeric antibodies are discussed by Neuberger et al (1988, 8th International Biotechnology Symposium Part 2, 792-799).
  • Monoclonal antibodies are useful in the methods of the invention and are a homogenous population of antibodies specifically targeting a single epitope on an antigen.
  • Antigen binding fragments of antibodies such as Fab and Fab 2 fragments may also be used/provided as can genetically engineered antibodies and antibody fragments.
  • the variable heavy (V H ) and variable light (V L ) domains of the antibody are involved in antigen recognition, a fact first recognised by early protease digestion experiments. Further confirmation was found by “humanisation” of rodent antibodies.
  • Variable domains of rodent origin may be fused to constant domains of human origin such that the resultant antibody retains the antigenic specificity of the rodent parent antibody (Morrison et al (1984) Proc. Natl. Acad. Sd. USA 81, 6851-6855).
  • the antibody/fragment is a fully human antibody/fragment.
  • a fully human antibody/fragment is encoded by human nucleic acid sequence(s).
  • Fully human antibodies/fragments are devoid of non-human amino acid sequences.
  • phage display in which human antibody genes are expressed in phage display libraries
  • production of antibodies in transgenic mice engineered to have human antibody genes (described in Park and Smolen Advances in Protein Chemistry (2001) 56: 369-421).
  • genes encoding the VH and VL chains are generated by PCR amplification and cloning from “naive” human lymphocytes, and assembled into a library from which they can be expressed either as disulfide-linked Fab fragments or as single-chain Fv (scFv) fragments.
  • the Fab- or scFv-encoding genes are fused to a surface coat protein of filamentous bacteriophage and Fab or scFv capable of binding to the target of interest can then be identified by screening the library with antigen. Molecular evolution or affinity maturation procedures can be employed to enhance the affinity of the Fab/scFv fragment.
  • mice in which the endogenous murine Ig gene loci have been replaced by homologous recombination with their human homologues are immunized with antigen, and monoclonal antibody is prepared by conventional hybridoma technology, to yield fully human monoclonal antibody.
  • the antibody/fragment according to the present invention is a murine antibody/fragment.
  • the antibody/fragment may be prepared by phage display using a human nave antibody gene library.
  • the antibody/fragment is a mouse/human chimeric antibody/fragment (e.g., an antibody/antigen binding fragment comprising murine variable domains and human constant regions). In some embodiments, the antibody/fragment is a humanised antibody/fragment (e.g., an antibody/antigen binding fragment comprising murine CDRs and human framework and constant regions).
  • a mouse/human chimeric antibody/antigen binding fragment can be prepared from a mouse monoclonal antibody by the process of chimerisation, e.g. as described in Human Monoclonal Antibodies: Methods and Protocols, Michael Steinitz (Editor), Methods in Molecular Biology 1060, Springer Protocols, Humana Press (2014), in Chapter 8 thereof, in particular section 3 of Chapter 8.
  • a humanised antibody/antigen binding fragment can be prepared from a mouse antibody by the process of chimerisation, e.g. as described in Human Monoclonal Antibodies: Methods and Protocols, Michael Steinitz (Editor), Methods in Molecular Biology 1060, Springer Protocols, Humana Press (2014), in Chapter 7 thereof, in particular section 3.1 of Chapter 7 entitled ‘Antibody Humanization’.
  • variable domains that antigenic specificity is conferred by variable domains and is independent of the constant domains is known from experiments involving the bacterial expression of antibody fragments, all containing one or more variable domains.
  • variable domains include Fab-like molecules (Better et al (1988) Science 240, 1041); Fv molecules (Skerra et al (1988) Science 240, 1038); single-chain Fv (ScFv) molecules where the V H and V L partner domains are linked via a flexible oligopeptide (Bird et al (1988) Science 242, 423; Huston et al (1988) Proc. Natl. Acad. Sd.
  • ScFv molecules we mean molecules wherein the V H and V L partner domains are covalently linked, e.g. by a flexible oligopeptide.
  • Fab, Fv, ScFv and dAb antibody fragments can all be expressed in and secreted from E. coli , thus allowing the facile production of large amounts of the said fragments.
  • the present invention provides an antibody or antigen binding fragment which is capable of binding to IL-11.
  • the antibody or antigen binding fragment may be isolated.
  • An antigen-binding fragment according to the present invention may be any fragment of a polypeptide which is capable of binding to an antigen.
  • an antigen binding fragment comprises at least three light chain CDRs (i.e. LC-CDR1, LC-CDR2 and LC-CDR3; also referred to herein as LC-CDRs 1-3) and three heavy chain CDRs (i.e. HC-CDR1, HC-CDR2 and HC-CDR3; also referred to herein as HC-CDRs 1-3) which together define the antigen binding region of an antibody or antigen binding fragment.
  • an antigen binding fragment may comprise the light chain variable domain and heavy chain variable domain of an antibody or antigen binding fragment.
  • an antigen binding fragment may comprise the light chain polypeptide and heavy chain polypeptide of an antibody or antigen binding fragment.
  • the present invention also provides a chimeric antigen receptor (CAR) capable of binding to IL-11, comprising one or more antigen binding fragments or polypeptides according to the present invention.
  • CARs Chimeric Antigen Receptors
  • CAR structure and engineering is reviewed, for example, in Dotti et al., Immunol Rev (2014) 257(1), hereby incorporated by reference in its entirety.
  • Antigen-binding fragments according to the present invention are provided herein as the antigen-binding domain of a chimeric antigen receptor (CAR).
  • the CAR comprises a VL domain and a VH domain according to any embodiment of an antibody, antigen binding fragment or polypeptide described herein.
  • CARs may be combined with costimulatory ligands, chimeric costimulatory receptors or cytokines to further enhance T cell potency, specificity and safety (Sadelain et al., The basic principles of chimeric antigen receptor (CAR) design. Cancer Discov. 2013 April; 3(4): 388-398. doi:10.1158/2159-8290.CD-12-0548, specifically incorporated herein by reference).
  • a cell comprising a CAR according to the invention.
  • the CAR according to the present invention may be used to generate T cells. Engineering of CARs into T cells may be performed during culture, in vitro, for transduction and expansion, such as happens during expansion of T cells for adoptive T cell therapy.
  • bispecific antibodies and bispecific antigen binding fragments comprising an antibody or antigen binding fragment according to the present invention.
  • the bispecific antibodies or bispecific antigen binding fragments may comprise (i) an antibody or antigen binding fragment according to the present invention, and (ii) an antibody or antigen binding fragment specific for a target other than IL-11.
  • Bispecific antibodies/fragments may be provided in any suitable format, such as those formats described in Kontermann MAbs 2012, 4(2): 182-197, which is hereby incorporated by reference in its entirety.
  • a bispecific antibody or bispecific antigen binding fragment may be a bispecific antibody conjugate (e.g. an IgG2, F(ab′) 2 or CovX-Body), a bispecific IgG or IgG-like molecule (e.g.
  • Methods for producing bispecific antibodies include chemically crosslinking of antibodies or antibody fragments, e.g. with reducible disulphide or non-reducible thioether bonds, for example as described in Segal and Bast, 2001. Production of Bispecific Antibodies. Current Protocols in Immunology. 14:IV:2.13:2.13.1-2.13.16, which is hereby incorporated by reference in its entirety.
  • SPDP N-succinimidyl-3-(-2-pyridyldithio)-propionate
  • SPDP N-succinimidyl-3-(-2-pyridyldithio)-propionate
  • SPDP N-succinimidyl-3-(-2-pyridyldithio)-propionate
  • SPDP N-succinimidyl-3-(-2-pyridyldithio)-propionate
  • SPDP N-succinimidyl-3-(-2-pyridyldithio)-prop
  • bispecific antibodies and bispecific antigen binding fragments can also be produced recombinantly, by expression from e.g.
  • nucleic acid construct encoding polypeptides for the antigen binding molecules, for example as described in Antibody Engineering: Methods and Protocols, Second Edition (Humana Press, 2012), at Chapter 40: Production of Bispecific Antibodies: Diabodies and Tandem scFv (Hornig and Farber-Schwarz), or French, How to make bispecific antibodies, Methods Mol. Med. 2000; 40:333-339, the entire contents of both of which are hereby incorporated by reference.
  • Antibodies may be produced by a process of affinity maturation in which a modified antibody is generated that has an improvement in the affinity of the antibody for antigen, compared to an unmodified parent antibody.
  • Affinity-matured antibodies may be produced by procedures known in the art, e.g., Marks et al., Rio/Technology 10:779-783 (1992); Barbas et al. Proc Nat. Acad. Sci. USA 91:3809-3813 (1994); Schier et al. Gene 169:147-155 (1995); Yelton et al. J. Immunol. 155:1994-2004 (1995); Jackson et al., J. Immunol. 154(7):331 0-15 9 (1995); and Hawkins et al, J. Mol. Biol. 226:889-896 (1992).
  • the present invention provides antibodies described herein which have further undergone the process of chain shuffling, e.g. light chain shuffling and/or heavy chain shuffling.
  • Chain shuffling to improve antibody affinity is described in detail in Marks, Antibody Affinity Maturation by Chain Shuffling, Antibody Engineering Methods and Protocols, Humana Press (2004) Vol. 248, pp 327-343, which is hereby incorporated by reference in its entirety—in particular, light chain shuffling is described in detail at sections 3.1 and 3.2 thereof.
  • light chain shuffling heavy chain variable regions of antibodies are combined with a repertoire of light chain variable region partners to identify new VLNH combinations having high affinity for the target protein of interest. In this way, the antibody/fragment is optimised for very high binding affinity.
  • the antibody/fragment of the present invention comprises the CDRs (i.e. CDRs 1-3) of the VH and/or VL domains of an IL-11-binding antibody clone described herein, or a variant thereof.
  • the antibody/fragment of the present invention comprises HC-CDRs 1-3 of an IL-11-binding antibody clone described herein, or a variant thereof.
  • the antibody/fragment of the present invention comprises LC-CDRs 1-3 of an IL-11-binding antibody clone described herein, or a variant thereof.
  • HC-CDRs 1-3 and LC-CDRs 1-3 of the antibody clones of the present disclosure are defined according to VBASE2, as described in Retter et al., Nucl. Acids Res. (2005) 33 (suppl 1): D671-D674, which is hereby incorporated by reference in its entirety.
  • a variant of a CDR may comprise e.g. 1 or 2 or 3 substitutions in the amino acid sequence of the CDR.
  • a variant of a VL or VH domain may comprise e.g. 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 substitutions in the amino acid sequence of the domain.
  • the antibody/fragment of the present invention comprises HC-CDRs 1-3 of an IL-11-binding antibody clone described herein, or a variant thereof, and LC-CDRs 1-3 of an IL-11-binding antibody clone described herein, or a variant thereof.
  • the antibody/fragment of the present invention comprises the CDRs of the VH and/or VL domains of an IL-11-binding antibody clone described herein, or a variant thereof. In some aspects, the antibody/fragment of the present invention comprises the VH and/or VL domains of an IL-11-binding antibody clone described herein, or a variant thereof.
  • the antibody/fragment of the present invention comprises the CDRs of the VH and/or VL domains of a clone, or a variant thereof, selected from YU33-A2, YU33-B3/H3, YU33-B4/YU45-G2/A3, YU33-E3, YU33-E6, YU45-C11/A10, YU45-D11/F11, YU45-E11/E12, YU45-H11/D12, YU45-A12/G10, YU45-G1, YU45-B2, YU45-C2/A7/B10, YU45-D2/H2/C7/F3/C9/E1/E9/C10/G3/H9/C5/A2/A5, YU45-B3, YU45-E3, YU45-C8/E8, YU45-F8, YU45-G8/H6, YU45-A2,
  • YU45-C11/A10 selected from YU45-C11/A10, YU45-G1, YU45-E3, YU45-F8, YU45-F9, YU45-H10, YU45-F2, YU45-H3, YU45-G7, YU45-B6, YU45-C1, YU46-B6, YU46-E3, YU46-G8 or YU46-D3; or selected from YU33-B4/YU45-G2/A3, YU45-H11/D12, YU45-G1, YU45-D2/H2/C7/F3/C9/E1/E9/C10/G3/H9/C5/A2/A5, YU45-B3, YU45-F8, YU45-H10, YU46-A10, YU45-A8/C6, YU45-D9/D3, YU45
  • the antibody/fragment of the present invention comprises the VH and/or VL domains of a clone, or a variant thereof, selected from YU33-A2, YU33-B3/H3, YU33-B4/YU45-G2/A3, YU33-E3, YU33-E6, YU45-C11/A10, YU45-D11/F11, YU45-E11/E12, YU45-H11/D12, YU45-A12/G10, YU45-G1, YU45-B2, YU45-C2/A7/B10, YU45-D2/H2/C7/F3/C9/E1/E9/C10/G3/H9/C5/A2/A5, YU45-B3, YU45-E3, YU45-C8/E8, YU45-F8, YU45-G8/H6, YU45-H8, Y
  • YU45-C11/A10 selected from YU45-C11/A10, YU45-G1, YU45-E3, YU45-F8, YU45-F9, YU45-H10, YU45-F2, YU45-H3, YU45-G7, YU45-B6, YU45-C1, YU46-B6, YU46-E3, YU46-G8 or YU46-D3; or selected from YU33-B4/YU45-G2/A3, YU45-H11/D12, YU45-G1, YU45-D2/H2/C7/F3/C9/E1/E9/C10/G3/H9/C5/A2/A5, YU45-B3, YU45-F8, YU45-H10, YU46-A10, YU45-A8/C6, YU45-D9/D3, YU45
  • the antibody/fragment of the present invention comprises the CDRs of the VH and/or VL domains of a clone, or a variant thereof, selected from BSN-1H2, BSN-1H7, BSN-2E1, BSN-2F5, BSN-2G6, BSN-3C6, BSN-3C11, BSN-5A6, BSN-5B8, BSN-5F6, BSN-6F3, BSN-7D4, BSN-7E4, BSN-7F9, BSN-8C4 or BSN-8H11, e.g.
  • BSN-2E1 selected from one of BSN-2G6, BSN-3C6, BSN-5A6 or BSN-5B8; or selected from one of BSN-2G6, BSN-3C6, BSN-5B8 or BSN-7D4; or BSN-3C6.
  • the antibody/fragment of the present invention comprises the VH and/or VL domains of a clone, or a variant thereof, selected from BSN-1H2, BSN-1H7, BSN-2E1, BSN-2F5, BSN-2G6, BSN-3C6, BSN-3C11, BSN-5A6, BSN-5B8, BSN-5F6, BSN-6F3, BSN-7D4, BSN-7E4, BSN-7F9, BSN-8C4 or BSN-8H11, e.g.
  • BSN-2E1 selected from one of BSN-2G6, BSN-3C6, BSN-5A6 or BSN-5B8; or selected from one of BSN-2G6, BSN-3C6, BSN-5B8 or BSN-7D4; or BSN-3C6.
  • the antibody/fragment of the present invention comprises HC-CDRs 1-3 of the VH domain of an IL-11-binding antibody clone described herein, or a variant thereof. In some aspects, the antibody/fragment of the present invention comprises the VH domain of a clone, or a variant thereof.
  • the antibody/fragment of the present invention comprises LC-CDRs 1-3 of the VL domain of an IL-11-binding antibody clone described herein, or a variant thereof. In some aspects, the antibody/fragment of the present invention comprises the VL domain of a clone, or a variant thereof.
  • the antibody/fragment of the present invention comprises HC-CDRs 1-3 of the VH domain, or the VH domain, of an IL-11-binding antibody clone selected from YU33-A2, YU33-B3/H3, YU33-B4/YU45-G2/A3, YU33-E3, YU33-E6, YU45-C11/A10, YU45-D11/F11, YU45-E11/E12, YU45-H11/D12, YU45-A12/G10, YU45-G1, YU45-B2, YU45-C2/A7/B10, YU45-D2/H2/C7/F3/C9/E1/E9/C10/G3/H9/C5/A2/A5, YU45-B3, YU45-E3, YU45-C8/E8, YU45-F8, YU45-G8/H6,
  • YU45-C11/A10 selected from YU45-C11/A10, YU45-G1, YU45-E3, YU45-F8, YU45-F9, YU45-H10, YU45-F2, YU45-H3, YU45-G7, YU45-B6, YU45-C1, YU46-B6, YU46-E3, YU46-G8 or YU46-D3; or selected from YU33-B4/YU45-G2/A3, YU45-H11/D12, YU45-G1, YU45-D2/H2/C7/F3/C9/E1/E9/C10/G3/H9/C5/A2/A5, YU45-B3, YU45-F8, YU45-H10, YU46-A10, YU45-A8/C6, YU45-D9/D3, YU45
  • the antibody/fragment of the present invention comprises LC-CDRs 1-3 of the VL domain, or the VL domain, of an IL-11-binding antibody clone selected from YU33-A2, YU33-B3/H3, YU33-B4/YU45-G2/A3, YU33-E3, YU33-E6, YU45-C11/A10, YU45-D11/F11, YU45-E11/E12, YU45-H11/D12, YU45-A12/G10, YU45-G1, YU45-B2, YU45-C2/A7/B10, YU45-D2/H2/C7/F3/C9/E1/E9/C10/G3/H9/C5/A2/A5, YU45-B3, YU45-E3, YU45-C8/E8, YU45-F8, YU45-G8/H6,
  • YU45-C11/A10 selected from YU45-C11/A10, YU45-G1, YU45-E3, YU45-F8, YU45-F9, YU45-H10, YU45-F2, YU45-H3, YU45-G7, YU45-B6, YU45-C1, YU46-B6, YU46-E3, YU46-G8 or YU46-D3; or selected from YU33-B4/YU45-G2/A3, YU45-H11/D12, YU45-G1, YU45-D2/H2/C7/F3/C9/E1/E9/C10/G3/H9/C5/A2/A5, YU45-B3, YU45-F8, YU45-H10, YU46-A10, YU45-A8/C6, YU45-D9/D3, YU45
  • the amino acid sequences of the VL domains of the human anti-human IL-11-binding antibody clones YU33-A2, YU33-B3/H3, YU33-B4/YU45-G2/A3, YU33-E3, YU33-E6, YU45-C11/A10, YU45-D11/F11, YU45-E11/E12, YU45-H11/D12, YU45-A12/G10, YU45-G1, YU45-B2, YU45-C2/A7/B10, YU45-D2/H2/C7/F3/C9/E1/E9/C10/G3/H9/C5/A2/A5, YU45-B3, YU45-E3, YU45-C8/E8, YU45-F8, YU45-G8/H6, YU45-H8, YU45-F9, YU45-H
  • the amino acid sequences of the VL domains and LC-CDRs 1-3 (defined using VBASE2) for these human anti-human IL-11-binding antibody clones are shown in FIG. 44
  • the amino acid sequences of the VH domains and HC-CDRs 1-3 (defined using VBASE2) for these human anti-human IL-11-binding antibody clones are shown in FIG. 45 .
  • Antibodies according to the present invention may comprise VL and/or VH chains comprising an amino acid sequence that has a high percentage sequence identity to one or more of the VL and/or VH amino acid sequences described herein.
  • antibodies according to the present invention include antibodies that bind IL-11 and have a VL chain that comprises an amino acid sequence having at least 70%, more preferably one of at least 75%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%, sequence identity to the VL chain amino acid sequence of one of SEQ ID NOs:1 to 50.
  • Antibodies according to the present invention include antibodies that bind IL-11 and have VH chain that comprises an amino acid sequence having at least 70%, more preferably one of at least 75%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%, sequence identity to the VH chain amino acid sequence of one of SEQ ID NOs:51 to 100.
  • Antibodies according to the present invention include antibodies that bind IL-11 and have a VL chain that comprises an amino acid sequence having at least 70%, more preferably one of at least 75%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%, sequence identity to the VL chain amino acid sequence of one of SEQ ID NOs: 267, 269, 270, 271, 274, 277, 279, 280, 540, 283, 286, 287, 289, 353, 293, 297, 299, 301, 303, 305, 307, 309, 310, 312, 314, 316, 318, 319, 321, 323, 325, 327, 328, 329, 331, 332, 335, 337, 338, 341, 342, 343, 346, 348, 214, 350, 13, 3, 351, 355, 358, 35, 361, 363, 365, 3
  • Antibodies according to the present invention include antibodies that bind IL-11 and have VH chain that comprises an amino acid sequence having at least 70%, more preferably one of at least 75%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%, sequence identity to the VH chain amino acid sequence of one of SEQ ID NOs: 53, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 382, 383, 384, 385, 386, 387, 388, 389, 85, 390, 73, 391, or 392.
  • the antibody/fragment of the present invention comprises HC-CDRs 1-3 of the VH domain, or the VH domain, of an IL-11-binding antibody clone selected from BSN-2E1, BSN-3C6, BSN-5A6_1 BSN-2G6, BSN-5A6_2 or BSN-5B8; e.g. BSN-3C6.
  • the antibody/fragment of the present invention comprises LC-CDRs 1-3 of the VL domain, or the VL domain, of an IL-11-binding antibody clone selected from BSN-2E1, BSN-3C6, BSN-5A6_1 BSN-2G6, BSN-5A6_2 or BSN-5B8; e.g. BSN-3C6.
  • the amino acid sequences of the VH domains of the anti-human IL-11-binding antibody clones BSN-2E1, BSN-3C6, BSN-5A6_1 BSN-2G6, BSN-5A6_2 and BSN-5B8 are shown in FIG. 68 , as are the HC-CDRs 1-3, defined using VBASE2 (described in Retter et al., Nucl. Acids Res. (2005) 33 (suppl 1): D671-D674).
  • the amino acid sequences of the VL domains for these anti-human IL-11-binding antibody clones are shown in FIG. 69 , as are the LC-CDRs 1-3, defined using VBASE2.
  • the antibody/fragment of the present invention comprises the CDRs of the VH and/or VL domains of a clone, or a variant thereof, selected from BSN-2E1, BSN-3C6, BSN-5A6_1 BSN-2G6, BSN-5A6_2 or BSN-5B8; e.g. BSN-3C6.
  • Antibodies according to the present invention include antibodies that bind IL-11 and have a VL chain that comprises an amino acid sequence having at least 70%, more preferably one of at least 75%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%, sequence identity to the VL chain amino acid sequence of one of SEQ ID NOs:554, 557, 561 or 564.
  • Antibodies according to the present invention include antibodies that bind IL-11 and have VH chain that comprises an amino acid sequence having at least 70%, more preferably one of at least 75%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%, sequence identity to the VH chain amino acid sequence of one of SEQ ID NOs: 541, 545, 548 or 551.
  • the light and heavy chain CDRs disclosed herein may also be particularly useful in conjunction with a number of different framework regions. Accordingly, light and/or heavy chains having LC-CDR1-3 or HC-CDR1-3 may possess an alternative framework region. Suitable framework regions are well known in the art and are described for example in M. Lefranc & G. Le:franc (2001) “The Immunoglobulin FactsBook”, Academic Press, incorporated herein by reference.
  • Antibodies according to the present invention may be detectably labelled or, at least, capable of detection.
  • the antibody may be labelled with a radioactive atom or a coloured molecule or a fluorescent molecule or a molecule which can be readily detected in any other way.
  • Suitable detectable molecules include fluorescent proteins, luciferase, enzyme substrates, radiolabels and binding moieties. Labelling may be by conjugation to the antibody/fragment.
  • the antigen binding molecule may be directly labelled with a detectable label or it may be indirectly labelled.
  • the label may be selected from: a radio-nucleotide, positron-emitting radionuclide (e.g. for positron emission tomography (PET)), MRI contrast agent or fluorescent label.
  • PET positron emission tomography
  • Antibodies and antigen binding fragments according to the present invention may be conjugated to a drug moiety, e.g. a cytotoxic small molecule. Such conjugates are useful for the targeted killing of cells expressing the antigen molecule.
  • a drug moiety e.g. a cytotoxic small molecule.
  • isolated heavy chain variable region polypeptides are also provided by the present invention.
  • an isolated heavy chain variable region polypeptide comprising the HC-CDRs 1-3 of any one of the anti-IL-11 antibody clones described herein.
  • an isolated heavy chain variable region polypeptide is provided, comprising an amino acid sequence having at least 70%, more preferably one of at least 75%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity to the amino acid sequence of the heavy chain variable region of any one of the anti-IL-11 antibody clones described herein.
  • an isolated light chain variable region polypeptide comprising the LC-CDRs 1-3 of any one of the anti-IL-11 antibody clones described herein.
  • an isolated light chain variable region polypeptide is provided, comprising an amino acid sequence having at least 70%, more preferably one of at least 75%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity to the amino acid sequence of the light chain variable region of any one of the anti-IL-11 antibody clones described herein.
  • Antibodies according to the present invention include antibodies that bind IL-11 and comprise an amino acid sequence having at least 70%, more preferably one of at least 75%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%, sequence identity to the amino acid sequence of one of SEQ ID NOs: 412 to 475.
  • IL-11 antibodies and fragments of the present invention may be characterised by reference to certain functional properties.
  • an IL-11 antibody or antigen binding fragment according to the present invention may possess one or more of the following properties:
  • inhibition refers to a reduction, decrease or lessening relative to a control condition.
  • inhibition of a process by an antibody/fragment refers to a reduction, decrease or lessening of the extent/degree of that process in the absence of the antibody/fragment, and/or in the presence of an appropriate control antibody/fragment.
  • Inhibition may herein also be referred to as neutralisation or antagonism. That is, an IL-11 binding antibody/fragment which is capable of inhibiting a function or process (e.g. interaction, signalling or other activity mediated by IL-11 or an IL-11-containing complex) may be said to be a ‘neutralising’ or ‘antagonist’ antibody/fragment with respect to the relevant function or process.
  • antibody/fragment which is capable of inhibiting IL-11 mediated signalling may be referred to as an antibody/fragment which is capable of neutralising IL-11 mediated signalling, or may be referred to as an antagonist of IL-11 mediated signalling.
  • control antibody/fragment may be an antibody/fragment directed against a target protein which is known not to have a role involved in the property being investigated in the assay.
  • a control antibody/fragment may be of the same isotype as the ant-IL-11 antibody/fragment being analysed, and may e.g. have the same constant regions.
  • an antibody/fragment that specifically binds to a target molecule preferably binds the target with greater affinity, and/or with greater duration than it binds to other, non-target molecules.
  • the present antibodies/fragments may bind with greater affinity to IL-11 than to one or more members of the IL-6 cytokine family.
  • the present antibodies/fragments may bind with greater affinity to IL-11 than to one or more of IL-6, leukemia inhibitory factor (LIF), oncostatin M (OSM), cardiotrophin-1 (CT-1), ciliary neurotrophic factor (CNTF), and cardiotrophin-like cytokine (CLC).
  • LIF leukemia inhibitory factor
  • OSM oncostatin M
  • CT-1 cardiotrophin-1
  • CNTF ciliary neurotrophic factor
  • CLC cardiotrophin-like cytokine
  • the extent of binding of an antibody to an non-target is less than about 10% of the binding of the antibody to the target as measured, e.g., by ELISA, SPR, Bio-Layer Interferometry (BLI), MicroScale Thermophoresis (MST), or by a radioimmunoassay (RIA).
  • the binding specificity may be reflected in terms of binding affinity, where the anti-IL-11 antibody/fragment of the present invention binds to IL-11 with a K D that is at least 0.1 order of magnitude (i.e. 0.1 ⁇ 10 n , where n is an integer representing the order of magnitude) greater than the K D towards another, non-target molecule. This may optionally be one of at least 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.5, or 2.0.
  • Binding affinity of an antibody or antigen-binding fragment for its target is often described in terms of its dissociation constant (K D ). Binding affinity can be measured by methods known in the art, such as by ELISA, Surface Plasmon Resonance (SPR; see e.g. Hearty et al., Methods Mol Biol (2012) 907:411-442; or Rich et al., Anal Biochem. 2008 Feb. 1; 373(1):112-20), Bio-Layer Interferometry (see e.g. Lad et al., (2015) J Biomol Screen 20(4): 498-507; or Concepcion et al., Comb Chem High Throughput Screen.
  • SPR Surface Plasmon Resonance
  • Bio-Layer Interferometry see e.g. Lad et al., (2015) J Biomol Screen 20(4): 498-507; or Concepcion et al., Comb Chem High Throughput Screen.
  • MST MicroScale Thermophoresis
  • the antibody/fragment according to the present invention binds to IL-11 with a K D of 5 ⁇ M or less, preferably one of ⁇ 1 ⁇ M, ⁇ 500 nM, ⁇ 100 nM, ⁇ 75 nM, ⁇ 50 nM, ⁇ 40 nM, ⁇ 30 nM, ⁇ 20 nM, ⁇ 15 nM, ⁇ 12.5 nM, ⁇ 10 nM, ⁇ 9 nM, ⁇ 8 nM, ⁇ 7 nM, ⁇ 6 nM, ⁇ 5 nM, ⁇ 4 nM, ⁇ 3 nM, ⁇ 2 nM, ⁇ 1 nM, ⁇ 500 pM.
  • Affinity of binding to IL-11 by an antibody/fragment may be analysed in vitro by ELISA assay.
  • Suitable assays are well known in the art and can be performed by the skilled person, for example, as described in Antibody Engineering, vol. 1 (2 nd Edn), Springer Protocols, Springer (2010), Part V, pp 657-665.
  • the affinity of binding to IL-11 by an antibody/fragment may be analysed according to the methodology described herein in the experimental examples.
  • an antibody/fragment to inhibit interaction between two proteins can be determined for example by analysis of interaction in the presence of, or following incubation of one or both of the interaction partners with, the antibody/fragment.
  • An example of a suitable assay to determine whether a given antibody/fragment is capable of inhibiting interaction between two interaction partners is a competition ELISA assay.
  • An antibody/fragment which is capable of inhibiting a given interaction is identified by the observation of a reduction/decrease in the level of interaction between the interaction partners in the presence of or following incubation of one or both of the interaction partners with the antibody/fragment, as compared to the level of interaction in the absence of the antibody/fragment (or in the presence of an appropriate control antibody/fragment).
  • Suitable analysis can be performed in vitro, e.g. using recombinant interaction partners or using cells expressing the interaction partners.
  • Cells expressing interaction partners may do so endogenously, or may do so from nucleic acid introduced into the cell.
  • one or both of the interaction partners and/or the antibody/fragment may be labelled or used in conjunction with a detectable entity for the purposes of detecting and/or measuring the level of interaction.
  • downstream functional consequences of interaction between IL-11 and IL-11Ra:gp130 or between IL-11:IL-11R ⁇ and gp130 may include proliferation of fibroblasts, myofibroblast generation from fibroblasts, or gene or protein expression of one or more of collagen, fibronectin, periostin, IL-6, IL-11, ⁇ SMA, TIMP1, MMP2.
  • Fibroblasts may be derived from any tissue, including liver, lungs, kidney, heart, blood vessels, eye, skin, pancreas, spleen, bowel (e.g. large or small intestine), brain, and bone marrow.
  • the fibroblasts may be cardiac fibroblasts (e.g. atrial fibroblasts), skin fibroblasts, lung fibroblasts, kidney fibroblasts or liver fibroblasts.
  • Fibroblasts may be characterised by gene or protein expression of one or more of COL1A, ACTA2, prolyl-4-hydroxylase, MAS516, and FSP1.
  • Gene expression can be measured by various means known to those skilled in the art, for example by measuring levels of mRNA by quantitative real-time PCR (qRT-PCR), or by reporter-based methods.
  • protein expression can be measured by various methods well known in the art, e.g. by antibody-based methods, for example by western blot, immunohistochemistry, immunocytochemistry, flow cytometry, ELISA, ELISPOT, or reporter-based methods.
  • the antibody/fragment according to the present invention may inhibit protein expression of one or more markers of fibrosis, e.g. protein expression of one or more of collagen, fibronectin, periostin, IL-6, IL-11, ⁇ SMA, TIMP1, MMP2.
  • markers of fibrosis e.g. protein expression of one or more of collagen, fibronectin, periostin, IL-6, IL-11, ⁇ SMA, TIMP1, MMP2.
  • an antibody/fragment to inhibit interaction between IL-11 and IL-11R ⁇ :gp130 can, for example, be analysed by stimulating fibroblasts with TGF ⁇ 1, incubating the cells in the presence of the antibody/fragment and analysing the proportion of cells having ⁇ SMA-positive phenotype after a defined period of time.
  • inhibition of interaction between IL-11 and IL-11R ⁇ :gp130 can be identified by observation of a lower proportion of cells having an ⁇ SMA-positive phenotype as compared to positive control condition in which cells are treated with TGF ⁇ 1 in the absence of the antibody/fragment (or in the presence of an appropriate control antibody/fragment), or in the presence of an appropriate control antibody/fragment.
  • Such assays are also suitable for analysing the ability of antibody/fragment to inhibit IL-11-mediated signalling.
  • the antibody/fragment according to the present invention is capable of inhibiting interaction between IL-11 and IL-11R ⁇ to less than 100%, e.g. one of 99% or less, 95% or less, 90% or less, 85% or less, 75% or less, 70% or less, 65% or less, 60% or less, 55% or less, 50% or less, 45% or less, 40% or less, 35% or less, 30% or less, 25% or less, 20% or less, 15% or less, 10% or less, 5% or less, or 1% or less of the level of interaction between IL-11 and IL-11R ⁇ in the absence of the antibody/fragment (or in the presence of an appropriate control antibody/fragment).
  • the antibody/fragment according to the present invention is capable of inhibiting interaction between IL-11 and IL-11R ⁇ to less than 1 times, e.g. one of ⁇ 0.99 times, ⁇ 0.95 times, ⁇ 0.9 times, ⁇ 0.85 times, ⁇ 0.8 times, ⁇ 0.85 times, ⁇ 0.75 times, ⁇ 0.7 times, ⁇ 0.65 times, ⁇ 0.6 times, ⁇ 0.55 times, ⁇ 0.5 times, ⁇ 0.45 times, ⁇ 0.4 times, ⁇ 0.35 times, ⁇ 0.3 times, ⁇ 0.25 times, ⁇ 0.2 times, ⁇ 0.15 times, ⁇ 0.1 times the level of interaction between IL-11 and IL-11R ⁇ in the absence of the antibody/fragment (or in the presence of an appropriate control antibody/fragment).
  • the antibody/fragment according to the present invention is capable of inhibiting interaction between IL-11 and gp130 to less than 100%, e.g. one of 99% or less, 95% or less, 90% or less, 85% or less, 75% or less, 70% or less, 65% or less, 60% or less, 55% or less, 50% or less, 45% or less, 40% or less, 35% or less, 30% or less, 25% or less, 20% or less, 15% or less, 10% or less, 5% or less, or 1% or less of the level of interaction between IL-11 and gp130 in the absence of the antibody/fragment (or in the presence of an appropriate control antibody/fragment).
  • the antibody/fragment according to the present invention is capable of inhibiting interaction between IL-11 and gp130 to less than 1 times, e.g. one of ⁇ 0.99 times, ⁇ 0.95 times, ⁇ 0.9 times, ⁇ 0.85 times, ⁇ 0.8 times, ⁇ 0.85 times, ⁇ 0.75 times, ⁇ 0.7 times, ⁇ 0.65 times, ⁇ 0.6 times, ⁇ 0.55 times, ⁇ 0.5 times, ⁇ 0.45 times, ⁇ 0.4 times, ⁇ 0.35 times, ⁇ 0.3 times, ⁇ 0.25 times, ⁇ 0.2 times, ⁇ 0.15 times, ⁇ 0.1 times the level of interaction between IL-11 and gp130 in the absence of the antibody/fragment (or in the presence of an appropriate control antibody/fragment).
  • 1 times e.g. one of ⁇ 0.99 times, ⁇ 0.95 times, ⁇ 0.9 times, ⁇ 0.85 times, ⁇ 0.8 times, ⁇ 0.85 times, ⁇ 0.75 times, ⁇ 0.7 times, ⁇ 0.65 times, ⁇ 0.6 times, ⁇ 0.5
  • the antibody/fragment according to the present invention is capable of inhibiting interaction between IL-11 and IL-11R ⁇ :gp130 to less than 100%, e.g. one of 99% or less, 95% or less, 90% or less, 85% or less, 75% or less, 70% or less, 65% or less, 60% or less, 55% or less, 50% or less, 45% or less, 40% or less, 35% or less, 30% or less, 25% or less, 20% or less, 15% or less, 10% or less, 5% or less, or 1% or less of the level of interaction between IL-11 and IL-11R ⁇ :gp130 in the absence of the antibody/fragment (or in the presence of an appropriate control antibody/fragment).
  • the antibody/fragment according to the present invention is capable of inhibiting interaction between IL-11 and IL-11R ⁇ :gp130 to less than 1 times, e.g. one of ⁇ 0.99 times, ⁇ 0.95 times, ⁇ 0.9 times, ⁇ 0.85 times, ⁇ 0.8 times, ⁇ 0.85 times, ⁇ 0.75 times, ⁇ 0.7 times, ⁇ 0.65 times, ⁇ 0.6 times, ⁇ 0.55 times, ⁇ 0.5 times, ⁇ 0.45 times, ⁇ 0.4 times, ⁇ 0.35 times, ⁇ 0.3 times, ⁇ 0.25 times, ⁇ 0.2 times, ⁇ 0.15 times, ⁇ 0.1 times the level of interaction between IL-11 and IL-11R ⁇ :gp130 in the absence of the antibody/fragment (or in the presence of an appropriate control antibody/fragment).
  • the antibody/fragment according to the present invention is capable of inhibiting interaction between IL-11:IL-11R ⁇ complex and gp130 to less than 100%, e.g. one of 99% or less, 95% or less, 90% or less, 85% or less, 75% or less, 70% or less, 65% or less, 60% or less, 55% or less, 50% or less, 45% or less, 40% or less, 35% or less, 30% or less, 25% or less, 20% or less, 15% or less, 10% or less, 5% or less, or 1% or less of the level of interaction between IL-11:IL-11R ⁇ complex and gp130 in the absence of the antibody/fragment (or in the presence of an appropriate control antibody/fragment).
  • the antibody/fragment is capable of inhibiting interaction between IL-11:IL-11R ⁇ complex and gp130 to less than 1 times, e.g. one of ⁇ 0.99 times, ⁇ 0.95 times, ⁇ 0.9 times, ⁇ 0.85 times, ⁇ 0.8 times, ⁇ 0.85 times, ⁇ 0.75 times, ⁇ 0.7 times, ⁇ 0.65 times, ⁇ 0.6 times, ⁇ 0.55 times, ⁇ 0.5 times, ⁇ 0.45 times, ⁇ 0.4 times, ⁇ 0.35 times, ⁇ 0.3 times, ⁇ 0.25 times, ⁇ 0.2 times, ⁇ 0.15 times, ⁇ 0.1 times the level of interaction between IL-11:IL-11R ⁇ complex and gp130 in the absence of the antibody/fragment.
  • Inhibition of IL-11 mediated signalling can also be analysed using 3 H-thymidine incorporation and/or Ba/F3 cell proliferation assays such as those described in e.g. Curtis et al. Blood, 1997, 90(11) and Karpovich et al. Mol. Hum. Reprod. 2003 9(2): 75-80.
  • Ba/F3 cells co-express IL-11R ⁇ and gp130.
  • IL-11 mediated signalling and/or processes mediated by IL-11 includes signalling mediated by fragments of IL-11 and polypeptide complexes comprising IL-11 or fragments thereof.
  • IL-11 mediated signalling may be signalling mediated by human IL-11 and/or mouse IL-11. Signalling mediated by IL-11 may occur following binding of IL-11 or an IL-11 containing complex to a receptor to which IL-11 or said complex binds.
  • antibodies and fragments according to the present invention are capable of inhibiting the biological activity of IL-11 or an IL-11-containing complex.
  • the antibody/fragment binds to IL-11 or the IL-11-containing complex in a region which is important for binding to a receptor for the IL-11 or IL-11-containing complex, e.g. gp130 or IL-11R ⁇ , and thereby disrupts binding to and/or signalling through the receptor.
  • the antibody/fragment according to the present invention is an antagonist of one or more signalling pathways which are activated by signal transduction through receptors comprising IL-11R ⁇ and/or gp130, e.g. IL-11R ⁇ :gp130.
  • the antibody/fragment is capable of inhibiting signalling through one or more immune receptor complexes comprising IL-11R ⁇ and/or gp130, e.g. IL-11R ⁇ :gp130.
  • the antibody/fragment according to the present invention is capable of inhibiting IL-11-mediated signalling to less than 100%, e.g. one of 99% or less, 95% or less, 90% or less, 85% or less, 75% or less, 70% or less, 65% or less, 60% or less, 55% or less, 50% or less, 45% or less, 40% or less, 35% or less, 30% or less, 25% or less, 20% or less, 15% or less, 10% or less, 5% or less, or 1% or less of the level of signalling in the absence of the antibody/fragment (or in the presence of an appropriate control antibody/fragment).
  • the antibody/fragment is capable of reducing IL-11 mediated signalling to less than 1 times, e.g.
  • the IL-11 mediated signalling may be signalling mediated by binding of IL-11 to IL-11R ⁇ :gp130 receptor.
  • Such signalling can be analysed e.g. by treating cells expressing IL-11R ⁇ and gp130 with IL-11, or by stimulating IL-11 production in cells which express IL-11R ⁇ and gp130.
  • the IC 50 for antibody/fragment for inhibition of IL-11 mediated signalling may be determined, e.g. by culturing Ba/F3 cells expressing IL-11R ⁇ and gp130 in the presence of human IL-11 and the IL-11 binding agent, and measuring 3 H-thymidine incorporation into DNA.
  • the antibody/fragment of the present invention may exhibit an IC 50 of 10 ⁇ g/ml or less, preferably one of ⁇ 5 ⁇ g/ml, ⁇ 4 ⁇ g/ml, ⁇ 3.5 ⁇ g/ml, ⁇ 3 ⁇ g/ml, ⁇ 2 ⁇ g/ml, ⁇ 1 ⁇ g/ml, ⁇ 0.9 ⁇ g/ml, ⁇ 0.8 ⁇ g/ml, ⁇ 0.7 ⁇ g/ml, ⁇ 0.6 ⁇ g/ml, or 0.5 ⁇ g/ml in such an assay.
  • the IL-11 mediated signalling may be signalling mediated by binding of IL-11:IL-11Ra complex to gp130.
  • the IL-11:IL-11R ⁇ complex may be soluble, e.g. complex of extracellular domain of IL-11R ⁇ and IL-11, or complex of soluble IL-11R ⁇ isoform/fragment, and IL-11.
  • the soluble IL-11R ⁇ is a soluble (secreted) isoform of IL-11R ⁇ , or is the liberated product of proteolytic cleavage of the extracellular domain of cell membrane bound IL-11R ⁇ .
  • the IL-11:IL-11R ⁇ complex may be cell-bound, e.g. complex of cell-membrane bound IL-11R ⁇ and IL-11.
  • Signalling mediated by binding of IL-11:IL-11R ⁇ complex to gp130 can be analysed by treating cells expressing gp130 with IL-11:IL-11R ⁇ complex, e.g. recombinant fusion protein comprising IL-11 joined by a peptide linker to the extracellular domain of IL-11R ⁇ (e.g. hyper IL-11 as described herein).
  • the antibody/fragment according to the present invention is capable of inhibiting signalling mediated by binding of IL-11:IL-11R ⁇ complex to gp130, and is also capable of inhibiting signalling mediated by binding of IL-11 to IL-11R ⁇ :gp130 receptor.
  • the antibody/fragment is capable of inhibiting fibroblast proliferation.
  • Proliferation of fibroblasts can be determined by analysing cell division over a period of time. Cell division for a given population of fibroblasts can be analysed, for example, by in vitro analysis of incorporation of 3 H-thymidine or by CFSE dilution assay, e.g. as described in Fulcher and Wong, Immunol Cell Biol (1999) 77(6): 559-564, hereby incorporated by reference in entirety. Proliferating cells (e.g.
  • proliferating fibroblasts may also be identified by analysis of incorporation of 5-ethynyl-2′-deoxyuridine (EdU) by an appropriate assay, as described e.g. in Buck et al., Biotechniques. 2008 June; 44(7):927-9, and Sali and Mitchison, PNAS USA 2008 Feb. 19; 105(7): 2415-2420, both hereby incorporated by reference in their entirety.
  • EdU 5-ethynyl-2′-deoxyuridine
  • Fibroblasts may be derived from any tissue, including liver, lungs, kidney, heart, blood vessels, eye, skin, pancreas, spleen, bowel (e.g. large or small intestine), brain, and bone marrow.
  • the fibroblasts may be cardiac fibroblasts (e.g. atrial fibroblasts), skin fibroblasts, lung fibroblasts, kidney fibroblasts or liver fibroblasts.
  • the antibody/fragment according to the present invention is capable of inhibiting fibroblast proliferation to less than 100%, e.g. one of 99% or less, 95% or less, 90% or less, 85% or less, 75% or less, 70% or less, 65% or less, 60% or less, 55% or less, 50% or less, 45% or less, 40% or less, 35% or less, 30% or less, 25% or less, 20% or less, 15% or less, 10% or less, 5% or less, or 1% or less of the level of fibroblast proliferation in the absence of the antibody/fragment (or in the presence of an appropriate control antibody/fragment).
  • the antibody/fragment is capable of reducing fibroblast proliferation to less than 1 times, e.g.
  • the antibody/fragment according to the present invention is capable of inhibiting a pathological process mediated by IL-11, e.g. following stimulation with a profibrotic factor (e.g. TGF ⁇ 1).
  • Pathological processes mediated by IL-11 include fibrosis, and can be evaluated either in vitro or in vivo.
  • the antibody/fragment according to the present invention is capable of inhibiting fibrosis.
  • Fibrosis may be of a particular tissue or several tissues, e.g. liver, lung, kidney, heart, blood vessel, eye, skin, pancreas, spleen, bowel (e.g. large or small intestine), brain, or bone marrow.
  • Fibrosis may be measured by means well known to the skilled person, for example by analysing gene or protein expression of one or more myofibroblast markers and/or gene or protein expression of one or more markers of fibrosis in a given tissue or tissues.
  • Myofibroblast markers may include one or more of increased ⁇ SMA, vimentin, palladin, cofilin or desmin. Markers of fibrosis include increased level of collagen, fibronectin, periostin, IL-6, IL-11, ⁇ SMA, TIMP1 and MMP2, extracellular matrix components, number/proportion of myofibroblasts, and organ weight.
  • Inhibition of fibrosis can be measured in vitro or in vivo. For example, whether an antibody/fragment is capable of inhibiting fibrosis in a given tissue can be analysed in vitro by treating fibroblasts derived from that tissue with a profibrotic stimulus, and then analysing whether the antibody can reduce myofibroblast generation from the fibroblasts (or e.g. some other marker of fibrosis). Whether an antibody/fragment is capable of inhibiting fibrosis can be analysed in vivo, for example, by administering the antibody/fragment to a subject (e.g. a subject that has been exposed to a profibrotic stimulus), and analysing tissue(s) for one or more markers of fibrosis.
  • a subject e.g. a subject that has been exposed to a profibrotic stimulus
  • the antibody/fragment according to the present invention is capable of inhibiting fibrosis to less than 100%, e.g. one of 99% or less, 95% or less, 90% or less, 85% or less, 75% or less, 70% or less, 65% or less, 60% or less, 55% or less, 50% or less, 45% or less, 40% or less, 35% or less, 30% or less, 25% or less, 20% or less, 15% or less, 10% or less, 5% or less, or 1% or less of the level of fibrosis in the absence of the antibody/fragment (or in the presence of an appropriate control antibody/fragment).
  • the antibody/fragment is capable of reducing fibrosis to less than 1 times, e.g.
  • the antibody/fragment according to the present invention is capable of inhibiting myofibroblast generation from fibroblasts, e.g. following exposure of the fibroblasts to profibrotic factor.
  • Myofibroblast generation from fibroblasts can be investigated by analysis for myofibroblast markers.
  • a profibrotic factor according to the present disclosure may be e.g. TGF ⁇ 1, IL-11, IL-13, PDGF, ET-1, oncostatin M (OSM) or ANG2 (AngII).
  • the antibody/fragment is capable of inhibiting gene or protein expression in fibroblasts, or fibroblast-derived cells (e.g. myofibroblasts), of one or more of collagen, fibronectin, periostin, IL-6, IL-11, ⁇ SMA, TIMP1, MMP2, e.g. following stimulation with a profibrotic factor.
  • the antibody/fragment is capable of inhibiting gene or protein expression in fibroblasts, or fibroblast-derived cells (e.g. myofibroblasts), of one or more extracellular matrix components, e.g. following stimulation with a profibrotic factor.
  • myofibroblast generation from fibroblasts is analysed by measuring ⁇ SMA protein expression levels using Operetta High-Content Imaging System following stimulation of the fibroblasts with TGF ⁇ 1.
  • the antibody/fragment according to the present invention is capable of inhibiting myofibroblast generation from fibroblasts to less than 100%, e.g. one of 99% or less, 95% or less, 90% or less, 85% or less, 75% or less, 70% or less, 65% or less, 60% or less, 55% or less, 50% or less, 45% or less, 40% or less, 35% or less, 30% or less, 25% or less, 20% or less, 15% or less, 10% or less, 5% or less, or 1% or less of the level of myofibroblast generation from fibroblasts in the absence of the antibody/fragment (or in the presence of an appropriate control antibody/fragment).
  • the antibody/fragment is capable of reducing myofibroblast generation from fibroblasts to less than 1 times, e.g. one of ⁇ 0.99 times, ⁇ 0.95 times, ⁇ 0.9 times, ⁇ 0.85 times, ⁇ 0.8 times, ⁇ 0.85 times, ⁇ 0.75 times, ⁇ 0.7 times, ⁇ 0.65 times, ⁇ 0.6 times, ⁇ 0.55 times, ⁇ 0.5 times, ⁇ 0.45 times, ⁇ 0.4 times, ⁇ 0.35 times, ⁇ 0.3 times, ⁇ 0.25 times, ⁇ 0.2 times, ⁇ 0.15 times, ⁇ 0.1 times the level of myofibroblast generation from fibroblasts in the absence of the antibody/fragment (or in the presence of an appropriate control antibody/fragment).
  • the antibody/fragment according to the present invention is capable of inhibiting gene or protein expression in fibroblasts of one or more of collagen, fibronectin, periostin, IL-6, IL-11, ⁇ SMA, TIMP1, MMP2, e.g. following stimulation with a profibrotic factor (e.g. TGF ⁇ 1).
  • the antibody/fragment according to the present invention is capable of inhibiting gene or protein expression to less than 100%, e.g.
  • the antibody/fragment is capable of reducing gene or protein expression to less than 1 times, e.g.
  • the antibody/fragment according to the present invention is capable of inhibiting extracellular matrix production by fibroblasts, e.g. following stimulation with a profibrotic factor (e.g. TGF ⁇ 1).
  • Extracellular matrix production can be evaluated, for example, by measuring the level of an extracellular matrix component.
  • Extracellular matrix components according to the present invention include e.g. proteoglycan, heparan sulphate, chondroitin sulphate, keratan sulphate, hyaluronic acid, collagen, periostin, fibronectin, vitronectin, elastin, fibronectin, laminin, nidogen, gelatin and aggrecan.
  • the antibody/fragment according to the present invention is capable of inhibiting extracellular matrix production by fibroblasts to less than 100%, e.g. one of 99% or less, 95% or less, 90% or less, 85% or less, 75% or less, 70% or less, 65% or less, 60% or less, 55% or less, 50% or less, 45% or less, 40% or less, 35% or less, 30% or less, 25% or less, 20% or less, 15% or less, 10% or less, 5% or less, or 1% or less of the level of extracellular matrix production by fibroblasts in the absence of the antibody/fragment (or in the presence of an appropriate control antibody/fragment).
  • the antibody/fragment is capable of reducing extracellular matrix production by fibroblasts to less than 1 times, e.g. one of ⁇ 0.99 times, ⁇ 0.95 times, ⁇ 0.9 times, ⁇ 0.85 times, ⁇ 0.8 times, ⁇ 0.85 times, ⁇ 0.75 times, ⁇ 0.7 times, ⁇ 0.65 times, ⁇ 0.6 times, ⁇ 0.55 times, ⁇ 0.5 times, ⁇ 0.45 times, ⁇ 0.4 times, ⁇ 0.35 times, ⁇ 0.3 times, ⁇ 0.25 times, ⁇ 0.2 times, ⁇ 0.15 times, ⁇ 0.1 times the level of extracellular matrix production in the absence of the antibody/fragment (or in the presence of an appropriate control antibody/fragment).
  • the antibody/fragment according to the present invention is capable of inhibiting proliferation and/or survival of cells of a cancer.
  • the skilled person is able to determine whether an antibody/fragment is capable of inhibiting proliferation and/or survival of cells of a cancer for example by analysing the effect of the antibody/fragment on cells of the cancer.
  • proliferation of cells can be measured as described herein, e.g. by 3 H thymidine incorporation or CFSE dilution assays.
  • Cell survival can be analysed by measuring cells for markers of cell viability/cell death following treatment with the antibody/fragment.
  • the antibody/fragment according to the present invention is capable of inhibiting proliferation and/or survival of cells of a cancer to less than 100%, e.g. one of 99% or less, 95% or less, 90% or less, 85% or less, 75% or less, 70% or less, 65% or less, 60% or less, 55% or less, 50% or less, 45% or less, 40% or less, 35% or less, 30% or less, 25% or less, 20% or less, 15% or less, 10% or less, 5% or less, or 1% or less of the level of proliferation and/or survival of cells of a cancer in the absence of the antibody/fragment (or in the presence of an appropriate control antibody/fragment).
  • the antibody/fragment is capable of reducing proliferation and/or survival of cells of a cancer to less than 1 times, e.g. one of ⁇ 0.99 times, ⁇ 0.95 times, ⁇ 0.9 times, ⁇ 0.85 times, ⁇ 0.8 times, ⁇ 0.85 times, ⁇ 0.75 times, ⁇ 0.7 times, ⁇ 0.65 times, ⁇ 0.6 times, ⁇ 0.55 times, ⁇ 0.5 times, ⁇ 0.45 times, ⁇ 0.4 times, ⁇ 0.35 times, ⁇ 0.3 times, ⁇ 0.25 times, ⁇ 0.2 times, ⁇ 0.15 times, ⁇ 0.1 times the level of proliferation and/or survival of cells of a cancer in the absence of the antibody/fragment (or in the presence of an appropriate control antibody/fragment).
  • the antibody/fragment according to the present invention is capable of inhibiting tumour growth to less than 100%, e.g. one of 99% or less, 95% or less, 90% or less, 85% or less, 75% or less, 70% or less, 65% or less, 60% or less, 55% or less, 50% or less, 45% or less, 40% or less, 35% or less, 30% or less, 25% or less, 20% or less, 15% or less, 10% or less, 5% or less, or 1% or less of the level of tumour growth in the absence of the antibody/fragment (or in the presence of an appropriate control antibody/fragment).
  • the antibody/fragment is capable of reducing tumour growth to less than 1 times, e.g.
  • the antibody/fragment according to the present invention has one or more improved properties as compared to a prior art anti-IL-11 antibody/fragment.
  • the prior art anti-IL-11 antibody/antigen binding fragment may be, or may comprise the CDRs and/or VL and VH sequences of, monoclonal mouse anti-human IL-11 antibody clone #22626; Catalog No. MAB218 (R&D Systems, MN, USA).
  • the antibody/fragment of the present invention displays one or more of the following properties as compared to a prior art antibody/antigen binding fragment (e.g. monoclonal mouse antibody clone #22626; Catalog No. MAB218):
  • “greater specificity” or “greater affinity” or “inhibition to a greater extent” herein is, respectively, a level of specificity, affinity or inhibition which is greater than 1 times, e.g. ⁇ 1.01 times, ⁇ 1.02 times, ⁇ 1.03 times, ⁇ 1.04 times, ⁇ 1.05 times, ⁇ 1.06 times, ⁇ 1.07 times, ⁇ 1.08 times, ⁇ 1.09 times, ⁇ 1.1 times, 21.2 times, ⁇ 1.3 times, ⁇ 1.4 times, ⁇ 1.5 times, ⁇ 1.6 times, ⁇ 1.7 times, ⁇ 1.8 times, ⁇ 1.9 times, ⁇ 2 times, ⁇ 2.1 times, ⁇ 2.2 times, ⁇ 2.3 times, ⁇ 2.4 times, ⁇ 2.5 times, ⁇ 2.6 times, ⁇ 2.7 times, ⁇ 2.8 times, ⁇ 2.9 times, ⁇ 3 times, ⁇ 3.5 times, ⁇ 4 times, ⁇ 4.5 times, ⁇ 5 times, ⁇ 6 times, ⁇ 7 times, ⁇ 8 times, ⁇ 9 times, ⁇ 10 times,
  • Antibodies and antigen binding fragments according to the present invention and compositions comprising such agents may be provided for use in methods of medical treatment or prevent of a disease/disorder, or alleviation of the symptoms of a disease/disorder.
  • the antibodies/fragments of the present invention may be administered to subjects having a disease/condition in need of treatment, and/or to subjects at risk of such developing or contracting the disease/disorder.
  • Treatment, prevention or alleviation of fibrosis according to the present invention may be of fibrosis that is associated with an upregulation of IL-11 and/or IL-11R ⁇ , e.g. an upregulation of IL-11 and/or IL-11R ⁇ in cells or tissue in which the disease/disorder occurs or may occur, or upregulation of extracellular IL-11 or IL-11R ⁇ .
  • IL-11 or IL-11R expression is locally or systemically upregulated in the subject.
  • Treatment or alleviation of a disease/disorder may be effective to prevent progression of the disease/disorder, e.g. to prevent worsening of the condition or to slow the rate of development.
  • treatment or alleviation may lead to an improvement in the disease/disorder, e.g. a reduction in the symptoms of the disease/disorder or reduction in some other correlate of the severity/activity of the disease/disorder.
  • Prevention of a disease/disorder may refer to prevention of a worsening of the condition or prevention of the development of the disease/disorder, e.g. preventing an early stage disease/disorder developing to a later, chronic, stage.
  • the antibodies/fragments of the present invention are preferably able to bind to and inhibit the biological activity of IL-11 and IL-11-containing molecules/complexes (e.g. IL-11:IL-11R ⁇ complex). Accordingly, the antibodies/fragments of the present invention find use in the treatment or prevention of diseases and disorders in which IL-11 is implicated in the pathology of the disease/disorder. That is, the antibodies/fragments of the present invention find use in the treatment or prevention of diseases and disorders associated with IL-11/IL-11R signalling.
  • IL-11 and IL-11-containing molecules/complexes e.g. IL-11:IL-11R ⁇ complex
  • the disease/disorder may be associated with increased IL-11, IL-11R ⁇ and/or gp130 gene or protein expression, e.g. as compared to the control (i.e. non-diseased) state.
  • the disease/disorder may be associated with an increased level of IL-11-mediated signalling as compared to the control state.
  • the disease/disorder may be associated with an increased level of signalling through ERK and/or STAT3 pathways as compared to the control state.
  • the increased expression/activity of IL-11, IL-11R ⁇ and/or gp130, and/or the increased level of IL-11-mediated signalling may be observed in effector cells of the disease/disorder (e.g. for a cancer, the cancerous cells). In some embodiments, the increased expression/activity of IL-11, IL-11R ⁇ and/or gp130, and/or the increased level of IL-11-mediated signalling, may be observed in cells other than the effector cells.
  • Signalling through ERK can be measured e.g. using an assay for ERK phosphorylation such as an assay described in Assay Guidance Manual: Phospho-ERK Assays, Kim E. Garbison, Beverly A. Heinz, Mary E. Lajiness, Jeffrey R. Weidner, and G. Sitta Sittampalam, E H Lilly & Company, Sittampalam GS, Coussens N P, Nelson H, et al., editors Bethesda (Md.): E H Lilly & Company and the National Center for Advancing Translational Sciences; 2004.
  • Signalling through STAT3 can be measured e.g. using an assay for phosphorylation of STAT3, such as the Phospho-STAT3 (Tyr705) Cellular Assay Kit (Cisbio Assays).
  • the treatment is of a disease/disorder for which a reduction in IL-11 mediated signalling is therapeutic. In some embodiments, the treatment is of a disease/disorder associated with excess ERK and/or STAT3 signalling. In some embodiments, the treatment is of a disease/disorder associated with excess proliferation or hyperactivation of fibroblasts, or associated with an excess of myofibroblasts.
  • the treatment may be aimed at preventing or treating a disease/disorder by decreasing the number or proportion of myofibroblasts or ⁇ SMA-positive fibroblasts.
  • the disease/disorder may be fibrosis, a fibrotic condition, or a disease/disorder characterised by fibrosis.
  • fibrosis refers to the formation of excess fibrous connective tissue as a result of the excess deposition of extracellular matrix components, for example collagen.
  • Fibrous connective tissue is characterised by having extracellular matrix (ECM) with a high collagen content.
  • ECM extracellular matrix
  • the collagen may be provided in strands or fibers, which may be arranged irregularly or aligned.
  • the ECM of fibrous connective tissue may also include glycosaminoglycans.
  • excess fibrous connective tissue refers to an amount of connective tissue at a given location (e.g. a given tissue or organ, or part of a given tissue or organ) which is greater than the amount of connective tissue present at that location in the absence of fibrosis, e.g. under normal, non-pathological conditions.
  • excess deposition of extracellular matrix components refers to a level of deposition of one or more extracellular matrix components which is greater than the level of deposition in the absence of fibrosis, e.g. under normal, non-pathological conditions.
  • the cellular and molecular mechanisms of fibrosis are described in Wynn, J. Pathol. (2008) 214(2): 199-210, and Wynn and Ramalingam, Nature Medicine (2012) 18:1028-1040, which are hereby incorporated by reference in their entirety.
  • the main cellular effectors of fibrosis are myofibroblasts, which produce a collagen-rich extracellular matrix.
  • pro-fibrotic factors such as TGF8, IL-13 and PDGF, which activate fibroblasts to ⁇ SMA-expressing myofibroblasts, and recruit myofibroblasts to the site of injury.
  • Myofibroblasts produce a large amount of extracellular matrix, and are important mediators in aiding contracture and closure of the wound.
  • myofibroblasts under conditions of persistent infection or during chronic inflammation there can be overactivation and recruitment of myofibroblasts, and thus over-production of extracellular matrix components, resulting in the formation of excess fibrous connective tissue.
  • fibrosis may be triggered by pathological conditions, e.g. conditions, infections or disease states that lead to production of pro-fibrotic factors such as TGF ⁇ 1.
  • fibrosis may be caused by physical injury/stimuli, chemical injury/stimuli or environmental injury/stimuli. Physical injury/stimuli may occur during surgery, e.g. iatrogenic causes.
  • Chemical injury/stimuli may include drug induced fibrosis, e.g. following chronic administration of drugs such as bleomycin, cyclophosphamide, amiodarone, procainamide, penicillamine, gold and nitrofurantoin (Daba et al., Saudi Med J 2004 June; 25(6): 700-6).
  • Environmental injury/stimuli may include exposure to asbestos fibres or silica.
  • Fibrosis can occur in many tissues of the body. For example, fibrosis can occur in the lung, liver (e.g. cirrhosis), kidney, heart, blood vessels, eye, skin, pancreas, spleen, bowel (e.g. large or small intestine), brain, and bone marrow. Fibrosis may also occur in multiple organs at once.
  • fibrosis may involve an organ of the gastrointestinal system, e.g. of the liver, small intestine, large intestine, or pancreas. In some embodiments, fibrosis may involve an organ of the respiratory system, e.g. the lungs. In embodiments, fibrosis may involve an organ of the cardiovascular system, e.g. of the heart or blood vessels. In some embodiments, fibrosis may involve the skin. In some embodiments, fibrosis may involve an organ of the nervous system, e.g. the brain. In some embodiments, fibrosis may involve an organ of the urinary system, e.g. the kidneys. In some embodiments, fibrosis may involve an organ of the musculoskeletal system, e.g. muscle tissue.
  • the fibrosis is cardiac or myocardial fibrosis, hepatic fibrosis, or renal fibrosis.
  • cardiac or myocardial fibrosis is associated with dysfunction of the musculature or electrical properties of the heart, or thickening of the walls or valves of the heart.
  • fibrosis is of the atrium and/or ventricles of the heart. Treatment or prevention of atrial or ventricular fibrosis may help reduce risk or onset of atrial fibrillation, ventricular fibrillation, or myocardial infarction.
  • hepatic fibrosis is associated with chronic liver disease or liver cirrhosis.
  • renal fibrosis is associated with chronic kidney disease.
  • fibrosis in accordance with the present invention, include but are not limited to: respiratory conditions such as pulmonary fibrosis, cystic fibrosis, idiopathic pulmonary fibrosis, progressive massive fibrosis, scleroderma, obliterative bronchiolitis, Hermansky-Pudlak syndrome, asbestosis, silicosis, chronic pulmonary hypertension, AIDS associated pulmonary hypertension, sarcoidosis, tumor stroma in lung disease, and asthma; chronic liver disease, primary biliary cirrhosis (PBC), schistosomal liver disease, liver cirrhosis; cardiovascular conditions such as hypertrophic cardiomyopathy, dilated cardiomyopathy (DCM), fibrosis of the atrium, atrial fibrillation, fibrosis of the ventricle, ventricular fibrillation, myocardial fibrosis, Brugada syndrome, myocarditis, endomyocardial fibrosis, my
  • respiratory conditions such as pulmonary
  • DR diabetic retinopathy
  • glaucoma glaucoma
  • subretinal fibrosis e.g, associated with macular degeneration (e.g. wet age-related macular degeneration (AMD))
  • macular edema e.g. drusen formation
  • post-surgical fibrosis e.g. of the posterior capsule following cataract surgery, or of the bleb following trabeculectomy for glaucoma
  • conjunctival fibrosis e.g. of the posterior capsule following cataract surgery, or of the bleb following trabeculectomy for glaucoma
  • conjunctival fibrosis e.g. of the posterior capsule following cataract surgery, or of the bleb following trabeculectomy for glaucoma
  • conjunctival fibrosis e.g. of the posterior capsule following cataract surgery, or of the bleb following trabeculectomy for glaucoma
  • fibrosis of the ventricle may occur post myocardial infarction, and is associated with DCM, HCM and myocarditis.
  • the disease/disorder may be one of pulmonary fibrosis, atrial fibrillation, ventricular fibrillation, hypertrophic cardiomyopathy (HCM), dilated cardiomyopathy (DCM), non-alcoholic steatohepatitis (NASH), cirrhosis, chronic kidney disease, scleroderma, systemic sclerosis, keloid, cystic fibrosis, Chron's disease, post-surgical fibrosis or retinal fibrosis, e.g. associated with wet age-related macular degeneration (AMD).
  • HCM hypertrophic cardiomyopathy
  • DCM dilated cardiomyopathy
  • NASH non-alcoholic steatohepatitis
  • Fibrosis can lead directly or indirectly to, and/or increase susceptibility to development of, diseases/disorders.
  • HCCs hepatocellular carcinomas
  • PME premalignant environment
  • the antibodies/fragments of the present invention find use in methods for the treatment and prevention of diseases/disorders associated with fibrosis, and/or for which fibrosis is a risk factor.
  • the disease/disorder associated with fibrosis, or for which fibrosis is a risk factor is a cancer, e.g. cancer of the liver (e.g. hepatocellular carcinoma).
  • IL-11 is also implicated in the pathology of other diseases/disorders, and the antibodies and fragments of the present invention accordingly find use in methods to treat, prevent and/or alleviate the symptoms of these diseases/disorders also.
  • IL-11 has been implicated in the development and progression of various cancers. Studies suggest that IL-11 is important for promoting chronic gastric inflammation and associated gastric, colonic, hepatocellular and breast cancer tumorogenesis through excessive activation of STAT3 (Ernst M, et al. J Olin Invest. (2008); 118:1727-1738), that IL-11 may promote tumorigenesis by triggering the JAK-STAT intracellular signalling pathway, and may also promote metastasis via signalling through the PI3K-AKT-mTORC1 pathway (Xu et al., Cancer Letters (2016) 373(2): 156-163).
  • IL-11 promotes survival, proliferation, invasion angiogenesis and metastasis
  • the IL-11/GP130/JAK/STAT3 signalling axis may be rate-limiting for the progression of gastrointestinal tumors, and elevated IL-11 expression is associated with poor prognosis of breast cancer patients (Johnstone et al., Cytokine & Growth Reviews (2015) 26(5): 489-498).
  • IL-11 has also been shown to influence breast cancer stem cell dynamics and tumor heterogeneity (Johnstone et al., Cytokine & Growth Reviews (2015) 26(5); 489-498).
  • IL-11 signalling has been implicated in chemoresistance of lung adenocarcinoma; cancer associated fibroblasts were found to upregulate IL-11, and confer chemoresistance to lung cancer cells through activation of the IL-11/IL-11RISTAT3 anti-apoptotic signalling pathway (Tao et al. 2016, Sci Rep. 6; 6:38408).
  • IL-11 signalling may promote the fibroblast-to-myofibroblast transition and extracellular matrix production by fibroblasts in the premalignant environment (PME) and tumour micro-environment (TME).
  • the antibodies/fragments of the present invention are provided for use in methods to treat/prevent a cancer.
  • the cancer may be a cancer which leads directly or indirectly to inflammation and/or fibrosis.
  • a cancer may be any unwanted cell proliferation (or any disease manifesting itself by unwanted cell proliferation), neoplasm or tumor or increased risk of or predisposition to the unwanted cell proliferation, neoplasm or tumor.
  • the cancer may be benign or malignant and may be primary or secondary (metastatic).
  • a neoplasm or tumor may be any abnormal growth or proliferation of cells and may be located in any tissue.
  • the antibodies/fragments of the present invention are provided for use in methods to treat/prevent a cancer, e.g. an epithelial cell cancer, breast cancer, gastrointestinal cancer (e.g. esophageal cancer, stomach cancer, pancreatic cancer, liver cancer (e.g. HOC), gallbladder cancer, colorectal cancer, anal cancer, gastrointestinal carcinoid tumor), and lung cancer (e.g. non-small cell lung cancer (NSCLC) or small cell lung cancer (SCLC))).
  • NSCLC non-small cell lung cancer
  • SCLC small cell lung cancer
  • the cancer is a cancer for which acute and/or chronic inflammation is a risk factor.
  • the cancer is a cancer for which a disease/disorder characterised by fibrosis (e.g. as described herein) is a risk factor.
  • the cancer may be associated with increased IL-11, IL-11R ⁇ and/or gp130 gene or protein expression.
  • cells of the cancer may have increased expression of IL-11, IL-11R ⁇ and/or gp130 as compared to comparable, non-cancerous cells, or may be associated with increased expression of IL-11, IL-11R ⁇ and/or gp130 by other cells (e.g. non-cancerous cells) as compared to the level of expression by comparable cells in the absence of a cancer (e.g. in a healthy control subject).
  • cells of the cancer may be determined to have an increased level of signalling through ERK and/or STATS pathways as compared to comparable non-cancerous cells.
  • the cancer may be associated with a mutation in IL-11, IL-11R ⁇ and/or gp130.
  • such mutation may be associated with increased level of gene or protein expression, or may be associated with an increased level of IL-11/IL-11R signalling relative to the level of expression/signalling observed in the absence of the mutation.
  • IL-11 has also been implicated in diseases/disorders characterised by inflammation. Intra-articular injection of IL-11 has been shown to cause joint inflammation (Wong et al., Cytokine (2005) 29:72-76), and IL-11 has been shown to be proinflammatory at sites of IL-13-mediated tissue inflammation (Chen et al., J Immunol (2005) 174:2305-2313). IL-11 expression has also been observed to be significantly increased in chronic skin lesions in atopic dermatitis, and is known to be involved in bronchial inflammation (Toda et al., J Allergy Clin Immunol (2003) 111:875-881).
  • IL-11-mediated signalling is implicated in inflammatory bowel disease (IBD) and asthma (Putoczki and Ernst, J Leuko Biol (2010) 88(6)1109-1117).
  • IBD inflammatory bowel disease
  • IL-11 has also been identified as a risk factor for multiple sclerosis; IL-11 is elevated in the cerebrospinal fluid of patients with clinically isolated syndrome (CIS) as compared to control subjects, and serum levels of IL-11 are higher during relapses for patients with relapsing-remitting multiple sclerosis, and IL-11 may promote differentiation of CD4+ T cells to a T H 17 phenotype T H 17 cells are important cells in the pathogenesis of multiple sclerosis (Zhang et al., Oncotarget (2015) 6(32): 32297-32298).
  • the antibodies/fragments of the present invention are provided for use in methods to treat/prevent a disease/disorder characterised by inflammation.
  • a disease or disorder characterised by inflammation may be a disease/disorder which leads directly or indirectly to a cancer and/or fibrosis.
  • Diseases characterised by inflammation include e.g.
  • allergic inflammation such as allergic asthma and bronchial inflammation, atopic dermatitis, allergic rhinitis and ocular allergic diseases, and autoimmune diseases such as multiple sclerosis, systemic lupus erythematosus, rheumatoid arthritis, chronic active hepatitis, type 1 diabetes mellitus, celiac disease, Grave's disease, uveitis, pemphigus, psoriasis, Crohn's disease, ulcerative colitis, inflammatory bowel disease, anaemia and autoimmune thyroiditis.
  • autoimmune diseases such as multiple sclerosis, systemic lupus erythematosus, rheumatoid arthritis, chronic active hepatitis, type 1 diabetes mellitus, celiac disease, Grave's disease, uveitis, pemphigus, psoriasis, Crohn's disease, ulcerative colitis, inflammatory bowel disease, anaemia and autoimmune thyroiditis
  • the antibodies/fragments of the present invention are provided for use in methods to treat/prevent a disease/disorder associated with infection, in particular where infection leads directly or indirectly to fibrosis, cancer or inflammation.
  • a disease associated with infection may be a disease which is caused or exacerbated by infection with the relevant infectious agent, or may be a disease for which infection with the relevant infectious agent is a risk factor.
  • An infection may be any infection or infectious disease, e.g. bacterial, viral, fungal, or parasitic infection.
  • the disease/disorder may be associated with a viral infection.
  • the infection may be chronic, persistent, latent or slow, and may be the result of bacterial, viral, fungal or parasitic infection.
  • treatment may be provided to patients having a bacterial, viral or fungal infection.
  • bacterial infections include infection with Helicobacter pylori and Mycobacterium tuberculosis infection of the lung.
  • viral infections include infection with EBV, HPV, HIV, hepatitis B or hepatitis C.
  • the treatment may involve ameliorating, treating, or preventing the disease/disorder by inhibiting the biological activity of IL-11 or an IL-11-containing complex.
  • Such methods may include the administration of the antibodies/fragments/compositions according to the present invention to bind to and inhibit the biological activity of IL-11 or an IL-11-containing complex.
  • inhibiting the biological activity of IL-11 or an IL-11-containing complex may be referred to as ‘neutralising’.
  • Methods of treatment may optionally include the co-administration of biological adjuvants (e.g., interleukins, cytokines, Bacillus Comette-Guerin, monophosphoryl lipid A, etc.) in combination with conventional therapies for treating cancer such as treatment with an agent for treating cancer (e.g. chemotherapy), radiation, or surgery.
  • biological adjuvants e.g., interleukins, cytokines, Bacillus Comette-Guerin, monophosphoryl lipid A, etc.
  • conventional therapies for treating cancer such as treatment with an agent for treating cancer (e.g. chemotherapy), radiation, or surgery.
  • Methods of medical treatment may also involve in vivo, ex vivo, and adoptive immunotherapies, including those using autologous and/or heterologous cells or immortalized cell lines.
  • the treatment may be aimed at prevention of a disease/disorder associated with overactive/elevated IL-11 mediated signalling.
  • the antibodies, antigen binding fragments and polypeptides may be used to formulate pharmaceutical compositions or medicaments and subjects may be prophylactically treated against development of a disease state. This may take place before the onset of symptoms of the disease state, and/or may be given to subjects considered to be at greater risk of the disease or disorder.
  • an antibody, antigen binding fragment or polypeptide is preferably in a “therapeutically effective amount”, this being sufficient to show benefit to the individual.
  • the actual amount administered, and rate and time-course of administration, will depend on the nature and severity of the disease being treated. Prescription of treatment, e.g. decisions on dosage etc., is within the responsibility of general practitioners and other medical doctors, and typically takes account of the disorder to be treated, the condition of the individual patient, the site of delivery, the method of administration and other factors known to practitioners. Examples of the techniques and protocols mentioned above can be found in Remington's Pharmaceutical Sciences, 20th Edition, 2000, pub. Lippincott, Williams & Wilkins.
  • Antibodies and antigen binding fragments according to the present invention may be formulated as pharmaceutical compositions or medicaments for clinical use and may comprise a pharmaceutically acceptable carrier, diluent, excipient or adjuvant.
  • compositions may be formulated for topical, parenteral, systemic, intracavitary, intravenous, intra-arterial, intramuscular, intrathecal, intraocular, intraconjunctival, intratumoral, subcutaneous, oral or transdermal routes of administration which may include injection or infusion.
  • Suitable formulations may comprise the antibody/fragment in a sterile or isotonic medium.
  • Medicaments and pharmaceutical compositions may be formulated in fluid, including gel, form. Fluid formulations may be formulated for administration by injection or via catheter to a selected region of the human or animal body.
  • methods are also provided for the production of pharmaceutically useful compositions, such methods of production may comprise one or more steps selected from: isolating an antibody or antigen binding fragment as described herein; and/or mixing an isolated antibody or antigen binding fragment as described herein with a pharmaceutically acceptable carrier, adjuvant, excipient or diluent.
  • a further aspect of the present invention relates to a method of formulating or producing a medicament or pharmaceutical composition for use in a method of medical treatment, the method comprising formulating a pharmaceutical composition or medicament by mixing an antibody or antigen binding fragment as described herein with a pharmaceutically acceptable carrier, adjuvant, excipient or diluent.
  • Antibodies, or antigen binding fragments, described herein may be used in methods that involve the binding of the antibody or antigen binding fragment to IL-11. Such methods may involve detection of the bound complex of antibody, or antigen binding fragment, and IL-11. As such, in one embodiment a method is provided, the method comprising contacting a sample containing, or suspected to contain, IL-11 with an antibody or antigen binding fragment as described herein and detecting the formation of a complex of antibody, or antigen binding fragment, and IL-11.
  • Suitable method formats are well known in the art, including immunoassays such as sandwich assays, e.g. ELISA.
  • the method may involve labelling the antibody/antigen binding fragment or IL-11, or both, with a detectable label, e.g. fluorescent, luminescent or radio-label.
  • IL-11 expression may be measured by immunohistochemistry (IHC), for example of a tissue sample obtained by biopsy.
  • the label may be selected from: a radio-nucleotide, positron-emitting radionuclide (e.g. for positron emission tomography (PET)), MRI contrast agent or fluorescent label.
  • Analysis in vitro or in vivo of processes mediated by IL-11 may involve analysis by positron emission tomography (PET), magnetic resonance imaging (MRI), or fluorescence imaging, e.g. by detection of appropriately labelled species.
  • PET positron emission tomography
  • MRI magnetic resonance imaging
  • fluorescence imaging e.g. by detection of appropriately labelled species.
  • Methods of this kind may provide the basis of a method of diagnosis of a disease or condition requiring detection and or quantitation of IL-11 or an IL-11-containing complex. Such methods may be performed in vitro on a subject sample, or following processing of a subject sample. Once the sample is collected, the subject is not required to be present for the in vitro method of diagnosis to be performed and therefore the method may be one which is not practised on the human or animal body.
  • Such methods may involve determining the amount of IL-11 or IL-11-containing complex present in a subject sample.
  • the method may further comprise comparing the determined amount against a standard or reference value as part of the process of reaching a diagnosis.
  • Other diagnostic tests may be used in conjunction with those described here to enhance the accuracy of the diagnosis or prognosis or to confirm a result obtained by using the tests described here.
  • the level of IL-11 or IL-11-containing complex present in a subject sample may be indicative that a subject may respond to treatment with an anti-IL-11 antibody/fragment, e.g. an anti-IL-11 antibody/fragment or composition according to the present invention.
  • an anti-IL-11 antibody/fragment e.g. an anti-IL-11 antibody/fragment or composition according to the present invention.
  • the presence of a high level of IL-11 or IL-11-containing complex in a sample may be used to select a subject for treatment with an anti-IL-11 antibody/fragment or composition described herein.
  • the antibodies of the present invention may therefore be used to select a subject for treatment with anti-IL-11 therapy.
  • Detection in a sample of IL-11 or IL-11-containing complex may be used for the purpose of diagnosis of an infectious disease, autoimmune disorder or a cancerous condition in the subject, diagnosis of a predisposition to an infectious disease, autoimmune disorder or a cancerous condition or for providing a prognosis (prognosticating) of an infectious disease, autoimmune disorder or a cancerous condition.
  • the diagnosis or prognosis may relate to an existing (previously diagnosed) infectious, inflammatory or autoimmune disease/disorder or cancerous condition.
  • a sample may be taken from any tissue or bodily fluid.
  • the sample may comprise or may be derived from: a quantity of blood; a quantity of serum derived from the individual's blood which may comprise the fluid portion of the blood obtained after removal of the fibrin clot and blood cells; a tissue sample or biopsy; pleural fluid; cerebrospinal fluid (CSF); or cells isolated from said individual.
  • the sample may be obtained or derived from a tissue or tissues which are affected by the disease/disorder (e.g. tissue or tissues in which symptoms of the disease manifest, or which are involved in the pathogenesis of the disease/disorder).
  • Methods according to the present invention may preferably be performed in vitro.
  • in vitro is intended to encompass experiments with cells in culture whereas the term “in vivo” is intended to encompass experiments with and/or treatment of intact multi-cellular organisms.
  • Antibodies, antigen binding fragments and compositions according to the present invention may be administered alone or in combination with other treatments. Administration of such combination may be simultaneous or sequential, depending on the disease/disorder to be treated.
  • the other treatment with which the antibody/fragment or composition is administered may be aimed at treating or preventing the disease/disorder.
  • the other treatment with which the antibody/fragment or composition is administered may be aimed at treating or preventing e.g. infection, inflammation and/or cancer.
  • Simultaneous administration refers to administration of the antibody, antigen binding fragment or polypeptide and therapeutic agent together, for example as a pharmaceutical composition containing both agents (combined preparation), or immediately after each other and optionally via the same route of administration, e.g. to the same artery, vein or other blood vessel.
  • Sequential administration refers to administration of one of the antibody, antigen binding fragment or polypeptide or therapeutic agent followed after a given time interval by separate administration of the other agent. It is not required that the two agents are administered by the same route, although this is the case in some embodiments.
  • the time interval may be any time interval.
  • treatment with an antibody, antigen binding fragment or composition of the present invention may be accompanied by an agent for treating or preventing infection (e.g. an antibiotic, anti-viral, anti-fungal or anti-parasitic agent).
  • treatment with an antibody, antigen binding fragment or composition of the present invention may be accompanied by an agent for treating or preventing inflammation (e.g. a non-steroidal anti-inflammatory drug (NSAID).
  • treatment with an antibody, antigen binding fragment or composition of the present invention may be accompanied by radiotherapy (i.e. treatment with ionising radiation, e.g. X-rays or ⁇ -rays) and/or an agent for treating or preventing cancer (e.g. a chemotherapeutic agent).
  • the antibody, antigen binding fragment or composition of the present invention may be administered as part of a combination treatment with an immunotherapy.
  • a treatment may involve administration of more than one drug.
  • a drug may be administered alone or in combination with other treatments, either simultaneously or sequentially dependent upon the condition to be treated.
  • Antibodies, antigen binding fragments, medicaments and pharmaceutical compositions according to aspects of the present invention may be formulated for administration by a number of routes, including but not limited to, parenteral, intravenous, intra-arterial, intraocular, intraconjunctival, intramuscular, subcutaneous, intradermal, intratumoral injection or infusion, and oral administration.
  • Antibodies, antigen binding fragments, polypeptides and other therapeutic agents may be formulated in fluid or solid form. Fluid formulations may be formulated for administration by injection or infusion to a selected region of the human or animal body.
  • kits of parts may have at least one container having a predetermined quantity of the antibody, fragment, or composition.
  • the kit may provide the antibody/fragment in the form of a medicament or pharmaceutical composition, and may be provided together with instructions for administration to a subject in order to treat a specified disease/disorder.
  • the antibody, fragment or composition may be formulated so as to be suitable for injection or infusion to a tumor or to the blood.
  • the kit may further comprise at least one container having a predetermined quantity of another therapeutic agent (e.g. anti-infective agent or chemotherapy agent).
  • the kit may also comprise a second medicament or pharmaceutical composition such that the two medicaments or pharmaceutical compositions may be administered simultaneously or separately such that they provide a combined treatment for the specific disease or condition.
  • the therapeutic agent may also be formulated so as to be suitable for injection or infusion to a tumor or to the blood.
  • the subject to be treated may be any animal or human.
  • the subject is preferably mammalian, more preferably human.
  • the subject may be a non-human mammal, but is more preferably human.
  • the subject may be male or female.
  • the subject may be a patient.
  • a subject may have been diagnosed with a disease or condition requiring treatment, or be suspected of having such a disease or condition.
  • the subject may be at risk of developing/contracting a disease or disorder.
  • the polypeptide may be expressed from a nucleotide sequence.
  • the nucleotide sequence may be contained in a vector present in a cell, or may be incorporated into the genome of the cell.
  • a “vector” as used herein is an oligonucleotide molecule (DNA or RNA) used as a vehicle to transfer exogenous genetic material into a cell.
  • the vector may be an expression vector for expression of the genetic material in the cell.
  • Such vectors may include a promoter sequence operably linked to the nucleotide sequence encoding the gene sequence to be expressed.
  • a vector may also include a termination codon and expression enhancers. Any suitable vectors, promoters, enhancers and termination codons known in the art may be used to express polypeptides from a vector according to the invention.
  • Suitable vectors include plasmids, binary vectors, viral vectors and artificial chromosomes (e.g. yeast artificial chromosomes).
  • operably linked may include the situation where a selected nucleotide sequence and regulatory nucleotide sequence (e.g. promoter and/or enhancer) are covalently linked in such a way as to place the expression of the nucleotide sequence under the influence or control of the regulatory sequence (thereby forming an expression cassette).
  • a regulatory sequence is operably linked to the selected nucleotide sequence if the regulatory sequence is capable of effecting transcription of the nucleotide sequence.
  • the resulting transcript may then be translated into a desired protein or polypeptide.
  • the cell may be a prokaryote or eukaryote.
  • Suitable prokaryotic cells include E. coli .
  • Examples of eukaryotic cells include a yeast cell, a plant cell, insect cell or a mammalian cell (e.g. Chinese Hamster Ovary (CHO) cells).
  • the cell is not a prokaryotic cell because some prokaryotic cells do not allow for the same post-translational modifications as eukaryotes.
  • very high expression levels are possible in eukaryotes and proteins can be easier to purify from eukaryotes using appropriate tags.
  • Specific plasmids may also be utilised which enhance secretion of the protein into the media.
  • Methods of producing a polypeptide of interest may involve culture or fermentation of a cell modified to express the polypeptide.
  • the culture or fermentation may be performed in a bioreactor provided with an appropriate supply of nutrients, air/oxygen and/or growth factors.
  • Secreted proteins can be collected by partitioning culture media/fermentation broth from the cells, extracting the protein content, and separating individual proteins to isolate secreted polypeptide. Culture, fermentation and separation techniques are well known to those of skill in the art.
  • Bioreactors include one or more vessels in which cells may be cultured. Culture in the bioreactor may occur continuously, with a continuous flow of reactants into, and a continuous flow of cultured cells from, the reactor. Alternatively, the culture may occur in batches.
  • the bioreactor monitors and controls environmental conditions such as pH, oxygen, flow rates into and out of, and agitation within the vessel such that optimum conditions are provided for the cells being cultured.
  • any suitable method for separating polypeptides from cell culture known in the art may be used. In order to isolate a polypeptide of interest from a culture, it may be necessary to first separate the cultured cells from media containing the polypeptide of interest. If the polypeptide of interest is secreted from the cells, the cells may be separated from the culture media that contains the secreted polypeptide by centrifugation. If the polypeptide of interest collects within the cell, it will be necessary to disrupt the cells prior to centrifugation, for example using sonification, rapid freeze-thaw or osmotic lysis. Centrifugation will produce a pellet containing the cultured cells, or cell debris of the cultured cells, and a supernatant containing culture medium and the polypeptide of interest.
  • polypeptide of interest may be desirable to isolate the polypeptide of interest from the supernatant or culture medium, which may contain other protein and non-protein components.
  • a common approach to separating polypeptide components from a supernatant or culture medium is by precipitation.
  • Polypeptides/proteins of different solubility are precipitated at different concentrations of precipitating agent such as ammonium sulfate.
  • precipitating agent such as ammonium sulfate
  • water soluble proteins are extracted.
  • Dialysis may be subsequently used to remove ammonium sulfate from the separated proteins.
  • polypeptide of interest Once the polypeptide of interest has been isolated from culture it may be necessary to concentrate the protein.
  • a number of methods for concentrating a protein of interest are known in the art, such as ultrafiltration or lyophilisation.
  • Alignment for purposes of determining percent amino acid or nucleotide sequence identity can be achieved in various ways known to a person of skill in the art, for instance, using publicly available computer software such as ClustalW 1.82. T-coffee or Megalign (DNASTAR) software. When using such software, the default parameters, e.g. for gap penalty and extension penalty, are preferably used.
  • the invention includes the combination of the aspects and preferred features described except where such a combination is clearly impermissible or expressly avoided.
  • FIG. 1 Graph showing read depth for whole transcriptome sequencing of human atrial fibroblasts from 160 individuals with and without stimulation with TGF ⁇ 1.
  • FIG. 2A PECAM1,
  • FIG. 2B MYH6
  • FIG. 2C TNNT2,
  • FIG. 2D COL1A2,
  • FIG. 2E ACTA2.
  • FIGS. 3A to 3E Graphs showing upregulation of IL-11 expression in fibroblasts in response to stimulation with TGF ⁇ 1.
  • FIG. 3A and FIG. 3B Graphs showing fold change in gene expression in fibrosis; IL-11 is the most upregulated gene in response to TGF ⁇ 1 treatment.
  • FIG. 3C IL-11 secretion by fibroblasts in response to stimulation with TGF ⁇ 1.
  • FIG. 3D Comparison of IL-11 gene expression in tissues of healthy individuals and in atrial fibroblasts, with or without TGF ⁇ 1 stimulation.
  • FIG. 3E Correspondence of fold change in IL-11 expression as determined by RNA-seq vs. qPCR.
  • FIGS. 4A to 4D Graphs showing induction of IL-11 secretion in primary fibroblasts by various profibrotic cytokines, as determined by ELISA.
  • FIG. 4A TGF ⁇ 1, ET-1, AngII, PDGF, OSM and IL-13 induce IL-11 secretion, and IL-11 also induces IL-11 expression in a positive feedback loop.
  • FIG. 4B Graph showing that the ELISA only detects native IL-11 secreted from cells, and does not detect recombinant IL-11 used for the IL-11 stimulation condition.
  • FIG. 4C and FIG. 4D Cells were stimulated with recombinant IL-11, IL-11RNA was measured and the native IL-11 protein level was measured in the cell culture supernatant by ELISA at the indicated time points.
  • FIGS. 5A to 5F Graphs and images showing myofibroblast generation from, and production of ECM and cytokine expression by, atrial fibroblasts in response to stimulation with TGF ⁇ 1 or IL-11.
  • FIG. 5A myofibroblast generation and ECM production by primary atrial fibroblasts following stimulation with TGF ⁇ 1 or IL-11, as measured by fluorescence microscopy following staining for a ⁇ -SMA, collagen or periostin.
  • FIG. 5B Collagen content of cell culture supernatant as determined by Sirius Red staining. Secretion of the fibrosis markers
  • FIG. 5C IL-6
  • FIG. 5D TIMP1 and
  • FIG. 5E MMP2 as measured by ELISA.
  • FIG. 5F Activation of murine fibroblasts by stimulation with human or mouse recombinant IL-11. *P ⁇ 0.05, **P ⁇ 0.01, ***P ⁇ 0.001, ****P ⁇ 0.0001 [Mean ⁇ SD, Dunnett].
  • FIGS. 6A to 6C Graphs showing the profibrotic effect of IL-11.
  • FIG. 6A Mouse fibroblasts from different tissues of origin can be activated by IL-11 and display increased ECM production. [Mean ⁇ SD, Dunnett]. Injection of mice with recombinant IL-11 or AngII results in ( FIG. 6B ) an increase in organ weight [Mean ⁇ SEM], and ( FIG. 6C ) an increase in collagen content (as determined by HPA assay). *P ⁇ 0.05, **P ⁇ 0.01, ***P ⁇ 0.001, ****P ⁇ 0.0001 [Mean ⁇ SD, Dunnett].
  • FIGS. 7A to 7F Graphs and images showing that IL-11 is required the pro-fibrotic effects of TGF ⁇ 1 on fibroblasts.
  • FIG. 7A myofibroblast generation and ECM production by primary atrial fibroblasts, with or without stimulation with TGF ⁇ 1, and in the presence/absence of neutralising anti-IL-11 antibody or isotype control IgG, as measured by fluorescence microscopy following staining for ( FIG. 7A ) ⁇ -SMA, ( FIG. 7B ) EdU or ( FIG. 7C ) Periostin.
  • FIGS. 7D to 7F Secretion of the fibrosis markers
  • FIG. 7D IL-6
  • FIG. 7E TIMP1
  • FIG. 7F MMP2 was analysed by ELISA. Fluorescence was normalized to the control group without stimulation. [Mean ⁇ SD, Dunnett]*P ⁇ 0.05, **P ⁇ 0.01, ***P ⁇ 0.001 or ****P ⁇ 0.0001.
  • FIGS. 8A and 8B Graphs and images showing the effect of neutralisation of IL-11 on collagen production triggered by TGF ⁇ 1.
  • FIG. 8A Operetta assay
  • FIG. 8B Sirius Red staining.
  • FIG. 9 Graphs showing the ability of various IL-11 and IL-11R ⁇ antagonists to inhibit fibrosis.
  • Human atrial fibroblasts were treated with neutralizing antibody against IL-11, neutralizing antibody against IL-11R ⁇ , decoy IL-11 receptor molecule that binds to IL-11, siRNA that downregulates IL-11 expression or siRNA that downregulates IL-11RA expression and the effect on the TGF ⁇ 1-driven pro-fibrotic response in fibroblasts in vitro was analysed.
  • FIGS. 10A to 10D Bar charts showing the response of fibroblasts from IL-11-RA knockout mice to pro-fibrotic treatment. Fibroblasts derived from IL-11RA WT (+/+), Heterozygous (+/ ⁇ ) and Homozygous null ( ⁇ / ⁇ ) mice were incubated for 24 h with TGF ⁇ 1, IL-11 or AngII (5 ng/ml).
  • FIG. 10A Percentage of myofibroblasts as determined by analysis ⁇ SMA content
  • FIG. 10B Percentage proliferating cells as determined by staining for EdU
  • FIG. 10C Collagen content
  • FIG. 10D ECM production as measured by detection of periostin [Mean ⁇ SD].
  • FIGS. 11A and 11B Graphs showing the effect of IL-11 neutralisation on fibrosis in response to various pro-fibrotic stimuli. Fibroblasts were cultured in vitro in the presence/absence of various different pro-fibrotic factors, and in the presence/absence of neutralising anti-IL-11 antibody or pan anti-TGF6 antibody ( FIG. 11A ) Collagen production and ( FIG. 11B ) myofibroblast generation as determined by analysis of ⁇ SMA expression. [Mean ⁇ SD, Dunnett]*P ⁇ 0.05, **P ⁇ 0.01, ***P ⁇ 0.001 or ****P ⁇ 0.0001.
  • FIGS. 12A to 12D Bar charts showing expression of markers of fibrosis in the atrium and heart of WT and IL-11RA ( ⁇ / ⁇ ) animals following treatment with AngII treatment.
  • FIG. 12A Collagen content, as measured by hydroxyproline assay.
  • FIG. 12B Collagen (Col1A2) expression.
  • FIG. 12C ⁇ SMA (ACTA2) expression.
  • FIG. 12D Fibronectin (Fn1) expression.
  • FIGS. 13A to 13C Schematics of the experimental procedures for analysing fibrosis in ( FIG. 13A ) lung, ( FIG. 13B ) skin and ( FIG. 13C ) eye for IL-11RA ⁇ / ⁇ mice as compared to IL-11RA+/+ mice.
  • FIGS. 14A and 14B Scatterplots showing fold change in gene expression.
  • FIG. 14A Fold changes in gene expression in fibroblasts following stimulation with TGF ⁇ 1, IL-11 or TGF ⁇ 1 and IL-11.
  • FIG. 14B Fold changes in gene expression in fibroblasts obtained from IL-11RA+/+ and IL-11RA ⁇ / ⁇ mice following stimulation with TGF ⁇ 1.
  • FIG. 15 Light chain variable domain sequences for human anti-IL-11 antibody clones. CDRs are underlined and shown separately.
  • FIG. 16 Heavy chain variable domain sequences for human anti-IL-11 antibody clones. CDRs are underlined and shown separately.
  • FIG. 17 Table showing light chain CDR sequences for human anti-IL-11 antibody clones.
  • FIG. 18 Table showing heavy chain CDR sequences for human anti-IL-11 antibody clones.
  • FIGS. 19A to 19C Tables showing light chain CDR sequences for human anti-IL-11 antibody clones and consensus sequences, for ( FIG. 19A ) LC-CDR1, ( FIG. 19B ) LC-CDR2 and ( FIG. 19C ) LC-CDR3.
  • FIGS. 20A to 20C Tables showing heavy chain CDR sequences for human anti-IL-11 antibody clones and consensus sequences, for ( FIG. 20A ) HC-CDR1, ( FIG. 20B ) HC-CDR2 and ( FIG. 20C ) HC-CDR3.
  • FIG. 21 Table summarising panning strategies used to identify human anti-human IL-11 antibodies capable of binding to both human IL-11 and mouse IL-11.
  • FIG. 22 Scatterplot showing strength of binding signal to human IL-11 and mouse IL-11 as determined by ELISA assay for 86 human anti-IL-11 antibody candidates.
  • FIG. 23 Table summarising the 56 human anti-human IL-11 antibody clones.
  • FIGS. 24A and 24B Bar charts showing inhibition by the human anti-IL-11 antibodies of signalling mediated by IL-11 in vitro in human atrial fibroblasts, as determined by fold change in the percentage of ⁇ SMA positive cells as compared to control (unstimulated) fibroblasts, following stimulation with TGF ⁇ 1, in the presence of the human anti-IL-11 antibodies.
  • FIG. 24B Bar chart showing the percentage of ⁇ SMA-positive cells (activated fibroblasts).
  • FIGS. 25A and 25B Bar chart showing inhibition by the human anti-IL-11 antibodies of signalling mediated by IL-11 in vitro in ( FIG. 25A ) mouse atrial fibroblasts and ( FIG. 25B ) mouse dermal fibroblasts, as determined by fold change in the percentage of ⁇ SMA positive cells as compared to control (unstimulated) fibroblasts, following stimulation with TGF ⁇ 1, in the presence of the human anti-IL-11 antibodies.
  • FIG. 26 Bar chart showing inhibition by the human anti-IL-11 antibodies of IL-11 trans signalling mediated by hyper IL-11 in vitro in human atrial fibroblasts, as determined by fold change in the percentage of ⁇ SMA positive cells as compared to control (unstimulated) fibroblasts, following stimulation with hyper IL-11, in the presence of the human anti-IL-11 antibodies.
  • FIG. 27 Table summarising the fold-change data of FIGS. 24 to 26 for the 56 human anti-IL-11 antibodies.
  • Antibody candidates numbered 1 to 56 correspond to clone designations as indicated in FIG. 23 .
  • Industry standard is monoclonal mouse anti-IL-11 IgG2A; Clone #22626; Catalog No. MAB218; R&D Systems, MN, USA.
  • FIGS. 28A to 28F Graphs showing binding of human anti-IL-11 antibodies to human IL-11 as determined by ELISA analysis.
  • FIG. 28A ELISA for clones YU45-A3, YU45-A10, YU45-D11, YU45-E11, YU45-D12 and YU33-A2(IgG).
  • FIG. 28B ELISA for clones YU45-G1, YU45-B2, YU45-A5, YU45-E3, YU45-F8 and YU33-H3(IgG).
  • FIG. 28A ELISA for clones YU45-G1, YU45-B2, YU45-A5, YU45-E3, YU45-F8 and YU33-H3(IgG).
  • FIG. 28C ELISA for clones YU45-G8, YU45-F9, YU45-H10, YU45-F2, YU45-H3 and YU33-E3(IgG).
  • FIG. 28D ELISA for clones YU45-A8, YU45-B5, YU45-D9, YU45-G7, YU45-B6 and YU45-F9.
  • FIG. 28E ELISA for clones YU45-F5, YU46-B5, YU45-C1, YU46-A8, YU46-B6 and YU45-F9.
  • FIG. 28F ELISA for clones YU46-E3, YU46-G8, YU46-D3, YU45-B6, YU45-C1 and YU45-F9.
  • FIG. 29 Table summarising EC 50 values determined for binding of human anti-IL-11 antibodies to IL-11 as determined by ELISA analysis.
  • FIG. 30 Schematic representation of the process of antibody light chain shuffling.
  • FIG. 31 Table summarising the 16 mouse anti-human IL-11 antibody clones.
  • FIG. 32 Bar chart showing inhibition by the mouse anti-IL-11 antibodies of signalling mediated by IL-11 in vitro in human atrial fibroblasts, as determined by fold change in the percentage of ⁇ SMA positive cells as compared to control (unstimulated) fibroblasts, following stimulation with TGF ⁇ 1, in the presence of the mouse anti-IL-11 antibodies.
  • FIG. 33 Bar chart showing inhibition by the mouse anti-IL-11 antibodies of signalling mediated by IL-11 in vitro in mouse atrial fibroblasts, as determined by fold change in the percentage of ⁇ SMA positive cells as compared to control (unstimulated) fibroblasts, following stimulation with TGF ⁇ 1, in the presence of the mouse anti-IL-11 antibodies.
  • FIG. 34 Bar chart showing inhibition by the mouse anti-IL-11 antibodies of IL-11 trans signalling mediated by hyper IL-11 in vitro in human atrial fibroblasts, as determined by fold change in the amount of MMP2 in the cell culture supernatant as compared to control (unstimulated) fibroblasts, following stimulation with hyper IL-11, in the presence of the mouse anti-IL-11 antibodies.
  • FIG. 35 Table summarising the fold-change data of FIGS. 32 to 34 for the 16 mouse anti-IL-11 antibodies.
  • Antibody candidates numbered 1 to 16 correspond to clone designations as indicated in FIG. 31 .
  • Industry standard is monoclonal mouse anti-IL-11 IgG2A; Clone #22626; Catalog No. MAB218; R&D Systems, MN, USA.
  • FIGS. 36A and 36B Table and bar chart showing binding of mouse-anti-IL-11 antibodies to human IL-11, as determined by iQue analysis
  • FIG. 36A Table summarising the results of the experiments.
  • FIG. 36B Bar chart showing strength of binding relative to the positive control anti-FLAG antibody (100%); numbers correspond to the clones as indicated in FIG. 35 .
  • FIG. 37 Graphs showing the effect of IL-11RA knockout on folate-induced kidney fibrosis as measured by collagen content in kidney tissue.
  • FIGS. 38A and 38B Photographs showing the effect of IL-11RA knockout on wound healing and fibrosis in the eye following trabeculectomy (filtration surgery).
  • FIG. 38A Eye sections of IL-11RA+/+(WT) and IL-11 RA ⁇ / ⁇ (KO) animals 7 days after filtration surgery.
  • FIG. 38B Maturation of collagen fibres as evaluated by picro-sirius red/polarization light technique (Szendroi et al. 1984, Acta Morphol Hung 32, 47-55); more fibrosis is observed in WT mice than KO mice.
  • FIGS. 39A to 39D Graphs showing that IL-11 is required the pro-fibrotic effects of TGF ⁇ 1 in liver fibroblasts.
  • FIG. 40 Bar chart showing that IL-11 is required for the pro-fibrotic effects of TGF ⁇ 1 in skin fibroblasts. Activation of mouse skin fibroblasts, with or without stimulation with TGF ⁇ 1, and in the presence/absence of neutralising anti-IL-11 antibody, as measured by analysis of the percentage of ⁇ -SMA positive cells (activated fibroblasts).
  • FIG. 41 Bar chart showing lung fibroblast cell migration with and without IL-11 signalling. Migration of lung fibroblasts from IL-11RA+/+(WT) and IL-11RA ⁇ / ⁇ (KO) animals was analysed in an in vitro scratch assay without stimulus, or in the presence of TGF ⁇ 1 or IL-11.
  • FIGS. 42A and 42B Graphs showing fibroblast activation in response to hyper IL-11. Cells were stimulated with the indicated amount (in ng/ml) of hyper IL-11 or recombinant IL-11, and fibroblast activation was measured by analysis of the percentage of ⁇ -SMA positive cells.
  • FIG. 42A and FIG. 42B present the results of two different experiments.
  • FIG. 43 Graph showing induction of IL-11 secretion in primary fibroblasts by hyper IL-11. Cells were stimulated with hyper IL-11, and IL-11RNA and native IL-11 protein levels were measured in the cell culture supernatant by ELISA at the indicated time points.
  • FIG. 44 Light chain variable domain sequences for human anti-IL-11 antibody clones after light chain shuffling. CDRs are underlined and shown separately.
  • FIG. 45 Heavy chain variable domain sequences for human anti-IL-11 antibody clones after light chain shuffling. CDRs are underlined and shown separately.
  • FIG. 46 Table showing light chain CDR sequences for human anti-IL-11 antibody clones after light chain shuffling.
  • FIG. 47 Table showing heavy chain CDR sequences for human anti-IL-11 antibody clones after light chain shuffling.
  • FIGS. 48A to 48C Tables showing light chain CDR sequences for human anti-IL-11 antibody clones after light chain shuffling, and consensus sequences, for ( FIG. 48A ) LC-CDR1, ( FIG. 48B ) LC-CDR2 and ( FIG. 48C ) LC-CDR3.
  • FIGS. 49A to 49C Tables showing heavy chain CDR sequences for human anti-IL-11 antibody clones after light chain shuffling, and consensus sequences, for ( FIG. 49A ) HC-CDR1, ( FIG. 49B ) HC-CDR2 and ( FIG. 49C ) HC-CDR3.
  • FIG. 50 Single-chain variable antibody fragment (ScFv) amino acid sequences for human anti-IL-11 antibody clones after light chain shuffling.
  • FIG. 51 Nucleotide sequences encoding scFv for human anti-IL-11 antibody clones after light chain shuffling.
  • FIG. 52 Table summarising panning strategies used to identify human anti-human IL-11 antibodies capable of binding to both human IL-11 and mouse IL-11, after light chain shuffling.
  • FIG. 53 Scatterplot showing binding signal to human IL-11 and mouse IL-11 as determined by ELISA assay for light chain-shuffled human anti-IL-11 antibodies. 66 antibodies displaying cross-reactive binding to human IL-11 and mouse IL-11 were identified (black circles). Antibodies displaying binding to mouse IL-11 only are indicated by grey circles.
  • FIGS. 54A and 54B Bar chart ( FIG. 54A ) and Table ( FIG. 54B ) showing binding signal to human IL-11 and mouse IL-11 as determined by ELISA assay for the 64 unique light chain-shuffled human anti-IL-11 antibodies.
  • FIG. 55 Bar chart showing EC50 values in ng/ml for binding of the indicated light-chain shuffled anti-IL-11 antibodies to human IL-11, as determined by ELISA.
  • FIGS. 56A and 56B Bar chart showing the effect of anti-IL-11 antibodies on MMP2 secretion by human cardiac atrial fibroblasts in response to TGF ⁇ 1
  • FIGS. 56A and 56B show the results of two separate experiments.
  • Cells were cultured in the presence of TGF ⁇ 1 (5 ng/ml) for 24 hours, in the presence of the indicated light chain shuffled anti-IL-11 antibodies, or in the presence of human IgG1 isotype control.
  • Basal MMP2 secretion by the cells in culture was measured by culture in the absence of TGF ⁇ 1, in the presence of human IgG1 isotype control.
  • Horizontal lines show basal MMP2 secretion by cardiac atrial human fibroblasts cultured for 24 hours in the presence of human IgG1 isotype control antibody in the absence of TGF ⁇ 1 (NEG); and MMP2 secretion by cardiac atrial human fibroblasts cultured for 24 hours in the presence of 5 ng/ml TGF ⁇ and the human IgG1 isotype control antibody (POS).
  • FIGS. 58A and 58B Images and graph showing the results of histological analysis of kidney sections from mice subjected to different treatments in a mouse model of kidney fibrosis.
  • Kidney fibrosis was induced by intraperitoneal (IP) injection of folic acid (FA, 180 mg/kg) in vehicle (0.3M NaHCO 3 ) mice; control mice were administered vehicle alone.
  • Mice were administered isotype control IgG2 (20 mg/kg, 3 ⁇ per week, intraperitoneal), anti-IL-11 antibody (20 mg/kg, 3 ⁇ per week, intraperitoneally) from day 1 post folic acid injury and for the duration of the experiment.
  • FIG. 58A Images of Masson's Trichrome stained kidney sections. Fibrotic areas containing collagen appear darker as compared to healthy areas that appear lighter.
  • FIG. 58B Graphs showing semi-quantitative analysis of collagen area expressed as a percentage (%) of the total kidney area (graph). ***, P ⁇ 0.001 compared to FA+IgG, ANOVA.
  • FIG. 59 Graph showing the urinary albumin/creatine ratio in mice subjected to different treatments in a mouse model of kidney fibrosis.
  • Kidney fibrosis was induced by intraperitoneal (IP) injection of folic acid (FA, 180 mg/kg) in vehicle (0.3M NaHCO 3 ) mice; control mice were administered vehicle alone.
  • FA treated mice were administered isotype control IgG2 (20 mg/kg, 3 ⁇ per week, intraperitoneal) or anti-IL11 antibody (20 mg/kg, 3 ⁇ per week, intraperitoneal) from day 1 post folic acid injury and for the duration of the experiment.
  • Mice were placed in metabolic cages and urinary creatinine and albumin measured using commercial assays (Abcam) according to the manufacturer's instructions. ***, P ⁇ 0.001 compared to FA+IgG, ANOVA.
  • FIG. 60 Graph showing total collagen in kidney tissue in mice subjected to different treatments in a mouse model of kidney fibrosis.
  • Kidney fibrosis was induced by intraperitoneal (IP) injection of folic acid (FA, 180 mg/kg) in vehicle (0.3M NaHCO 3 ) mice; control mice were administered vehicle alone. From day one of the experiment, mice in the treatment groups were given isotype control IgG2 (20 mg/kg, 3 ⁇ per week) or neutralizing anti-IL11 antibody at varying doses: 20 mg/kg ⁇ 3/week; 10 mg/kg ⁇ 3/week; 10 mg/kg ⁇ 2/week; 5 mg/kg ⁇ 3/week; 5 mg/kg ⁇ 2/week; 1 mg/kg ⁇ 2/week), all intraperitoneal.
  • IgG2 isotype control IgG2 (20 mg/kg, 3 ⁇ per week) or neutralizing anti-IL11 antibody at varying doses: 20 mg/kg ⁇ 3/week; 10 mg/kg ⁇ 3/week; 10 mg/kg ⁇ 2/week; 5 mg/kg ⁇ 3/week;
  • FIGS. 61A and 61B Images and graph showing the results of histological analysis of kidney sections from mice subjected to different treatments in a mouse model of acute renal injury.
  • FIG. 61A Mice were treated by sham operation or ureteric obstruction of one ureter. Mice received IgG, anti-IL-11 antibody (20 mg/kg on surgical days ⁇ 1, 1, 3, 5) and injured kidneys (UUO IgG, IL-11) or contralateral (Con) uninjured kidneys (Con IgG, IL-11) were harvested on day 7 post surgery.
  • FIG. 61A Mice were treated by sham operation or ureteric obstruction of one ureter. Mice received IgG, anti-IL-11 antibody (20 mg/kg on surgical days ⁇ 1, 1, 3, 5) and injured kidneys (UUO IgG, IL-11) or contralateral (Con) uninjured kidneys (Con IgG, IL-11) were harvested on day 7 post surgery.
  • FIG. 61A Mice were treated by
  • FIG. 62 Image showing the results of ELISA western blot for IL-11 of human liver samples. Liver samples obtained from patients undergoing liver surgery were used for western blot analysis. Blotting of GAPDH was used as a loading control. Samples from normal human liver (NHL) had low levels of IL-11 protein, whereas samples from patients with fibrotic liver diseases including alcoholic liver disease (ALD), primary sclerosing cholangitis (PSC), primary biliary cirrhosis (PBC) or non-alcoholic steatohepatitis (NASH) had higher levels of IL-11.
  • ALD alcoholic liver disease
  • PSC primary sclerosing cholangitis
  • PBC primary biliary cirrhosis
  • NASH non-alcoholic steatohepatitis
  • FIG. 63 Bar chart showing the results of ELISA analysis of secretion of IL-11 by human PCLS subjected to different treatments.
  • FIGS. 64A and 64B Images and graph showing the results of analysis of liver tissue from mice subjected to different treatments in a mouse model of nonalcoholic steatohepatitis.
  • Diabetic mice db/db; deficient for the leptin receptor
  • MCD methionine/choline deficient
  • neutralizing anti-IL11 antibody was administered (20 mg/kg, 3 ⁇ /week, intraperitoneal) for the final 3 weeks of the 8 week NASH diet.
  • Liver samples were photographed ( FIG. 64A ) and assessed for collagen content per mg of liver tissue ( FIG. 64B ); each symbol represents an individual animal. P values shown on graph, ANOVA.
  • FIGS. 65A and 65B Bar chart and images showing the results of analysis of eye fibrosis from mice subjected to different treatments in a mouse model of retinal fibrosis. Mice (10 per group) were subjected to laser-induced retinal damage (4 burns per retina) and administered intraocularly with 0.5 ⁇ g of anti-IL-11 antibody or IgG control antibody on days 1, 7, 14 and 21. Eyes were harvested for histological analyses on day 28. The area of fibrosis at burn sites were measured by Masson's Trichrome staining.
  • FIG. 65A Bar cahrt showing quantification of the fibrosis areas in control (IgG) or anti-IL11 (IL11) treated mice.
  • FIG. 65B representative images showing staining of fibrotic areas in control antibody treated eyes (IGG, top panel) or anti-IL11 treated eyes (IL11, bottom panel).
  • FIGS. 66A to 66C Schematic, images and bar chart relating to analysis of skin fibrosis in mice subjected to different treatments in a mouse model of skin fibrosis.
  • FIG. 66A Schematic representation of experimental procedures for different treatment groups. Groups 1 and 2 were treated with bleomycin (BLM), and either anti-IL-11 antibody (Group 1) or IgG control antibody (Group 2). Group 3 were injected with vehicle (PBS) only and do not develop fibrosis.
  • FIG. 66B Images showing Masson's trichrome staining of skin section at equal distances from the injection site. Dermal thickness is indicated by the black bar.
  • FIG. 66A Schematic representation of experimental procedures for different treatment groups. Groups 1 and 2 were treated with bleomycin (BLM), and either anti-IL-11 antibody (Group 1) or IgG control antibody (Group 2). Group 3 were injected with vehicle (PBS) only and do not develop fibrosis.
  • FIG. 66B Images showing Masson's t
  • FIG. 67 Images showing the results of histological analysis of heart fibrosis in mice subjected to different treatments in a mouse model of cardiac fibrosis.
  • Mice C57616, male, 8-12 weeks old) were subjected to fibrosis-inducing transverse aortic constriction (TAC) or sham operations.
  • TAC-treated animals received either control antibody (20 mg/kg, 3x/week, intraperitoneal) or neutralizing anti-IL-11 antibody (20 mg/kg, 3x/week, intraperitoneal). After two weeks hearts were harvested and assessed for fibrosis extent using Masson's Trichrome stain.
  • FIG. 68 Heavy chain variable domain sequences for mouse anti-IL-11 antibody clones. CDRs are underlined and shown separately.
  • FIG. 69 Light chain variable domain sequences for mouse anti-IL-11 antibody clones. CDRs are underlined and shown separately.
  • FIG. 70 Table showing heavy chain CDR sequences for mouse anti-IL-11 antibody clones.
  • FIG. 71 Table showing light chain CDR sequences for mouse anti-IL-11 antibody clones.
  • FIGS. 72A to 72C Tables showing heavy chain CDR sequences for mouse anti-IL-11 antibody clones, and consensus sequences, for ( FIG. 72A ) HC-CDR1, ( FIG. 72B ) HC-CDR2 and ( FIG. 72C ) HC-CDR3.
  • FIGS. 73A to 73C Tables showing light chain CDR sequences for mouse anti-IL-11 antibody clones, and consensus sequences, for ( FIG. 73A ) LC-CDR1, ( FIG. 73B ) LC-CDR2 and ( FIG. 73C ) LC-CDR3.
  • FIG. 74 Nucleotide sequences encoding mouse anti-IL-11 antibody clone heavy chains and light chains.
  • the inventors identify a role for IL-11/IL-11R signalling in fibrosis in a variety of tissues, and described the generation of anti-human IL-11 antibodies, and in vitro and in vivo functional characterisation of the antibodies.
  • Example 1 A Role for IL-11 in Fibrosis
  • RNA-seq high-throughput RNA sequencing
  • Markers for fibroblasts COL1A2 ( FIG. 2D ) and ACTA2 ( FIG. 2E ) were highly expressed compared to the tissue of origin.
  • RNA-seq data was analysed to identify genes whose expression was increased or decreased upon stimulation with TGF ⁇ 1, and this information was integrated with the large RNA-seq dataset across 35+ human tissues provided by the GTEx project (The GTEx Consortium, 2015 Science 348, 648-660). This enabled the identification of gene expression signatures that were specific to the fibroblast-myofibroblast transition.
  • FIGS. 3A to 3E The results are shown in FIGS. 3A to 3E .
  • IL-11 was the most strongly upregulated gene in response to stimulation with TGF ⁇ 1, and on average across the 160 individuals was upregulated more than 10-fold ( FIG. 3B ).
  • IL-11 expression was confirmed by ELISA analysis of the cell culture supernatant of TGF ⁇ 1 stimulated fibroblasts ( FIG. 3C ). As compared to the level of expression level of IL-11 in other tissues of healthy individuals, this response was observed to be highly specific to activated fibroblasts ( FIG. 3D ). Various fold changes of IL-11RNA expression were also confirmed by qPCR analysis ( FIG. 3E ).
  • fibroblasts were cultured in vitro and stimulated with several other known pro-fibrotic factors: ET-1, ANGII, PDGF, OSM and IL-13, and also with human recombinant IL-11.
  • ET-1 pro-fibrotic factor
  • ANGII pro-fibrotic factor
  • PDGF vascular endothelial growth factor
  • OSM vascular endothelial growth factor
  • IL-13 human recombinant IL-11
  • FIG. 4A The results are shown in FIG. 4A .
  • IL-11 is shown to act in an autocrine loop in fibroblasts, which can result in an upregulation of IL-11 protein as much as 100-fold after 72 hours ( FIG. 4D ).
  • IL-6 is from the same cytokine family and also signals via the gp130 receptor (Garbers and Scheller, 2013 Biol Chem 394, 1145-1161), which is proposed to ensure the continued survival and growth of lung and breast cancer cells (Grivennikov and Karin, 2008 Cancer Cell 13, 7-9).
  • 1.2 IL-11 has a Profibrotic Role in Fibrosis of Heart Tissue
  • fibroblasts were cultured in vitro with recombinant IL-11, and the fraction of myofibroblasts ( ⁇ SMA-positive cells) and extracellular matrix production was analysed.
  • Atrial fibroblasts derived from 3 individuals were incubated in 2 wells each for 24 h without stimulation, with TGF ⁇ 1 (5 ng/ml), or with IL-11 (5 ng/ml). Following incubation, cells were stained to analyse ⁇ -SMA content to estimate the fraction of myofibroblasts, and for collagen and periostin to estimate ECM production. Fluorescence was measured in 7 fields per well. The supernatant of 2 wells per individual was also assessed for collagen content by Sirius Red staining. The signal was normalized to the control group without stimulation. Secretion of the fibrosis markers IL-6, TIMP1 and MMP2 was analysed via ELISA.
  • FIGS. 5A to 5F The results are shown in FIGS. 5A to 5F .
  • TGF ⁇ 1 activated fibroblasts and increased ECM production ( FIG. 5A ).
  • recombinant IL-11 caused an increase in the fraction of myofibroblasts in fibroblast cultures, and also promoted the production of extracellular matrix proteins collagen and periostin to the same extent as TGF ⁇ 1 ( FIG. 5A ).
  • Both of IL-11 and TGF ⁇ 1 cytokines also significantly increased the secretion of pro-fibrotic markers IL-6, TIMP1 and MMP2 ( FIGS. 5B to 5E ), and to a similar level.
  • IL-11 has a Profibrotic Role in Fibrosis of a Variety of Tissues
  • IL-11 profibrotic action of IL-11 was specific to atrial fibroblasts
  • human fibroblasts derived from several different tissues were cultured in vitro, stimulated with human IL-11, and fibroblast activation and ECM production was analysed as described above. Increased fibroblast activation and production of ECM was observed as compared to non-stimulated cultures in fibroblasts derived from each of the tissues analysed.
  • human primary liver fibroblasts (Cell Biologics, Cat#: H-6019) were cultured at low passage in wells of 96-well plates and either not stimulated, stimulated with TGF ⁇ 1 (5 ng/ml, 24h), IL-11 (5 ng/ml, 24h) or incubated with both TGF ⁇ 1 (5 ng/ml) and a neutralising IL-11 antibody (2 ⁇ g/ml), or TGF ⁇ 1 (5 ng/ml) and an Isotype control antibody. Fibroblast activation ( ⁇ SMA positive cells), cell proliferation (EdU positive cells) and ECM production (Periostin and Collagen) was analysed using the Operetta platform.
  • FIGS. 39A to 39D The results of the experiments with primary human liver fibroblasts are shown in FIGS. 39A to 39D .
  • IL-11 was found to activate liver fibroblasts, and IL-11 signalling was found to be necessary for the profibrotic action of TGF ⁇ 1 in liver fibroblasts. Both activation and proliferation of fibroblasts was inhibited by neutralising anti-IL-11 antibody.
  • IL-11 signalling is important in skin fibrosis
  • primary mouse skin fibroblasts were cultured at low passage in wells of 96-well plates and either not stimulated, stimulated with TGF ⁇ 1 (5 ng/ml, 24h) or incubated for 24 h with both TGF ⁇ 1 (5 ng/ml) and a neutralising IL-11 antibody (2 ⁇ g/ml).
  • Fibroblast activation was then analysed using the Operetta platform.
  • TGF ⁇ 1-mediated activation of skin fibroblasts was inhibited by neutralising anti-IL-11 antibody.
  • mouse recombinant IL-11 was injected (100 ⁇ g/kg, 3 days/week, 28 days) into mice to test whether IL-11 can drive global tissue fibrosis in vivo.
  • FIGS. 6A to 6C The results are shown in FIGS. 6A to 6C .
  • AngII a cytokine that causes an elevation in blood pressure and hypertrophy of the heart
  • IL-11 also increased the heart weight but also kidney, lung and liver weight indexed to body weight ( FIG. 6B ).
  • Assessing collagen content in these issues by hydroxyproline assay revealed an upregulation of collagen production in these tissues, indicating fibrosis as the likely cause for the increase in organ weight ( FIG. 6C ).
  • Example 2 Therapeutic Potential of IL-11/IL-11R Antagonism
  • IL-11 was inhibited using a commercially available neutralizing antibody (Monoclonal Mouse IgG2A; Clone #22626; Catalog No. MAB218; R&D Systems, MN, USA).
  • a commercially available neutralizing antibody Monoclonal Mouse IgG2A; Clone #22626; Catalog No. MAB218; R&D Systems, MN, USA.
  • Fibroblasts were treated with TGF ⁇ 1 in the presence or absence of the antibody, and fibroblast activation, the proportion of proliferating cells and ECM production and markers of the fibrotic response were measured.
  • Atrial fibroblasts derived from 3 individuals were incubated for 24 h with TGF ⁇ 1 (5 ng/ml) or TGF ⁇ 1 in the presence of neutralising anti-IL-11 antibody or isotype control antibody. Following incubation, cells were stained for ⁇ SMA to determine the fraction of myofibroblasts, the proportion of proliferating cells was determined by analysing the cells for EdU incorporation, and periostin was measured to determine ECM production. Fluorescence was measured with the Operetta platform for 14 fields across 2 wells for each individual. Secretion of the fibrosis markers IL-6, TIMP1 and MMP2 was also analysed by ELISA. Fluorescence was normalized to the control group without stimulation.
  • FIGS. 7A to 7F The results are shown in FIGS. 7A to 7F .
  • IL-11 inhibition was found to ameliorate TGF ⁇ 1-induced fibrosis, and it was shown that IL-11 is essential for the pro-fibrotic effect of TGF ⁇ 1. Inhibition of IL-11 was found to ‘rescue’ the TGF ⁇ 1 phenotype at the protein level.
  • Collagen production was also analysed. Cardiac fibroblasts derived from 3 individuals were incubated for 24 h with TGF ⁇ 1 (5 ng/ml) or TGF ⁇ 1 and a neutralizing IL-11 antibody. Following incubation the cells were stained for collagen using the Operetta assay and florescence was quantified as described above. Secreted collagen levels in the cell culture supernatant were assessed by Sirius Red staining.
  • FIGS. 8A and 8B The results are shown in FIGS. 8A and 8B , and confirm the anti-fibrotic effect of inhibition of IL-11 using a neutralising antibody.
  • human atrial fibroblasts cells were cultured in vitro, stimulated for 24 h with TGF ⁇ 1 (5 ng/ml) or left unstimulated, in the presence/absence of: (i) neutralising anti-IL-11 antibody, (ii) a IL-11RA-gp130 fusion protein (iii) neutralising anti-IL-11RA antibody, (iv) treatment with siRNA directed against IL-11 or (v) treatment with siRNA directed against IL-11RA.
  • TGF ⁇ 1 5 ng/ml
  • a IL-11RA-gp130 fusion protein iii) neutralising anti-IL-11RA antibody
  • siRNA directed against IL-11 or treatment with siRNA directed against IL-11RA.
  • the proportion of activated fibroblasts was analysed by evaluating ⁇ SMA content as described above.
  • Example 3 In Vivo Confirmation of a Profibrotic Role for IL-11/IL-11R Signalling
  • mice All mice were bred and housed in the same room and provided food and water ad libitum. Mice lacking functional alleles for IL-11R ⁇ (IL-11RA1 KO mice) were on C57131/6 genetic background. Mice were of 9-11 weeks of age and the weight of animals did not differ significantly.
  • primary fibroblasts were generated from IL-11RA gene knock-out mice and incubated with primary fibroblast cells harvested from IL-11RA+/+(i.e. wildtype), IL-11RA+/ ⁇ (i.e. heterozygous knockout) and IL-11RA ⁇ / ⁇ (i.e. homozygous knockout) animals with TGF ⁇ 1, IL-11 or AngII. Activation and proliferation of fibroblasts and ECM production was analysed.
  • Fibroblasts derived from IL-11RA+/+, IL-11RA+/ ⁇ and IL-11RA ⁇ / ⁇ mice were incubated for 24 hours with TGF ⁇ 1, IL-11 or AngII (5 ng/ml). Following incubation, cells were stained for ⁇ SMA content to estimate the fraction of myofibroblasts, for EdU to identify the fraction of proliferating cells, and for collagen and periostin to estimate ECM production. Fluorescence was measured using the Operetta platform.
  • FIGS. 10A to 10D The results are shown in FIGS. 10A to 10D .
  • IL-11RA ⁇ / ⁇ mice were found not to respond to pro-fibrotic stimuli. These results suggested that IL-11 signalling is also required for AngII-induced fibrosis.
  • fibroblasts were cultured in vitro in the presence/absence of various different pro-fibrotic factors (ANG2, ET-1 or PDGF), and in the presence/absence of neutralising anti-IL-11 antibody or pan anti-TGF ⁇ antibody. After 24 hours, collagen production by the cells was determined by analysis using the Operetta system as described above, and myofibroblast generation was determined by analysis of ⁇ SMA expression as described above.
  • pro-fibrotic factors ANG2, ET-1 or PDGF
  • IL-11 was found to be required for fibrosis downstream of various profibrotic stimuli, and was thus identified as a central mediator of fibrosis induced by a variety of different profibrotic factors.
  • IL-11 signalling was investigated in lung fibrosis, using an in vitro scratch assay of migration of lung fibroblasts.
  • fibroblasts are activated and migrate within the fibrotic niche in the body.
  • the migration rate of cells is a measure of cell-cell and cell-matrix interactions and a model for wound healing in vivo (Liang et al., 2007; Nat Protoc. 2(2):329-33).
  • Fibroblasts derived from lung tissue from both wild type (WT) and also homozygous IL-11RA ( ⁇ / ⁇ ) knockout mice were grown at low passage on a plastic surface until they formed a uniform cell monolayer. A scratch was then created in the cell layer, and cell migration close to the scratch was monitored, either in the absence of stimulation, or in the presence of TGF ⁇ 1 or IL-11. Images captured at images at the two time points of immediately after creating the scratch and at 24 h were used to determine the area covered by cells, and the rate of migration was compared between WT and KO fibroblasts. Cell migration (area in the scratch covered by cells after 24h) was normalized to the migration rate of WT cells without stimulus.
  • FIG. 41 Lung fibroblasts derived from WT mice were shown to migrate faster in the presence of TGF ⁇ 1 and IL-11, indicating a pro-fibrotic effect of both cytokines in lung fibroblasts.
  • Cells lacking IL-11 signalling derived from KO mice migrated more slowly as compared to WT cells. They also did not migrate faster in the presence of TGF ⁇ 1.
  • the scratch assay revealed that lung fibroblasts lacking IL-11 signalling have a decrease cell migration rate both in the presence of TGF ⁇ 1 or IL-11, and at baseline. Thus, inhibition of IL-11 signalling is anti-fibrotic in the lung.
  • IL-11 inhibition to treat fibrotic disorders was investigated in vivo.
  • a mouse model for cardiac fibrosis in which fibrosis is induced by treatment with AngII, was used to investigate whether IL-11RA ⁇ / ⁇ mice were protected from cardiac fibrosis.
  • a pump was implanted, and wildtype (WT) IL-11RA(+/+) and knockout (KO) IL-11RA( ⁇ / ⁇ ) mice were treated with AngII (2 mg/kg/day) for 28 days.
  • WT wildtype
  • KO knockout
  • IL-11RA( ⁇ / ⁇ ) mice were treated with AngII (2 mg/kg/day) for 28 days.
  • collagen content was assessed in the atria of the mice using a calorimetric hydroxyproline-based assay kit, and the level of RNA expression of the markers or fibrosis Col1A2, ⁇ SMA (ACTA2) and fibronectin (Fn1) were analysed by qPCR.
  • FIGS. 12A to 12D The results are shown in FIGS. 12A to 12D .
  • the IL-11RA ⁇ / ⁇ mice were found to be protected from the profibrotic effects of AngII.
  • a mouse model for kidney fibrosis was established in wildtype (WT) IL-11RA(+/+) and knockout (KO) IL-11 RA( ⁇ / ⁇ ) mice by intraperitoneal injection of folic acid (180 mg/kg) in vehicle (0.3M NaHCO 3 ); control mice were administered vehicle alone.
  • Kidneys were removed 28 days post-injection, weighed and either fixed in 10% neutral-buffered formalin for Masson's trichrome and Sirius staining or snap-frozen for collagen assay, RNA, and protein studies.
  • the snap-frozen kidneys were subjected to acid hydrolysis by heating in 6M HCl at a concentration of 50 mg/ml (95° C., 20 hours).
  • the amount of total collagen in the hydrolysate was quantified based on the colorimetric detection of hydroxyproline using Quickzyme Total Collagen assay kit (Quickzyme Biosciences) as per the manufacturer's instructions.
  • FIG. 37 The results of the analysis are shown in FIG. 37 .
  • Folate-induced kidney fibrosis is shown to be dependent on IL-11 mediated signalling.
  • a significant increase in collagen content in kidney tissue was observed in IL-11 RA+/+ mice, indicative of kidney fibrosis.
  • No significant increase in collagen content was observed in IL-11 RA ⁇ / ⁇ mice.
  • Animals deficient for IL-11 signalling had significantly less collagen deposition in kidneys after toxic injury as compared to wild type animals.
  • IL-11 is confirmed as a key mediator of fibrosis in the lung, skin and eye in further in vivo models using the IL-11RA ⁇ / ⁇ knockout mice. Schematics of the experiments are shown in FIGS. 13A to 13C .
  • IL-11RA ⁇ / ⁇ mice and IL-11RA+/+ mice are treated by intratracheal administration of bleomycin on day 0 to establish a fibrotic response in the lung (pulmonary fibrosis). Fibrosis of the lung develops by 21 days, at which point animals are sacrificed and analysed for differences in fibrosis markers between animals with and without IL-11 signalling.
  • IL-11RA ⁇ / ⁇ mice have a reduced fibrotic response in lung tissue as compared to IL-11RA+/+ mice, as evidenced by reduced expression of markers of fibrosis.
  • IL-11RA ⁇ / ⁇ mice and IL-11RA+/+ mice are treated by subcutaneous administration of bleomycin on day 0 to establish a fibrotic response in the skin. Fibrosis of the skin develops by 28 days, at which point animals are sacrificed and analysed for differences in fibrosis markers between animals with and without IL-11 signalling. IL-11RA ⁇ / ⁇ mice have a reduced fibrotic response in skin tissue as compared to IL-11RA+/+ mice, as evidenced by reduced expression of markers of fibrosis.
  • IL-11RA ⁇ / ⁇ mice and IL-11RA+/+ mice underwent trabeculectomy (filtration surgery) on day 0 to initiate a wound healing response in the eye.
  • This mouse model of glaucoma filtration surgery has been shown to be an efficient model to evaluate the wound healing response in the eye (Khaw et al. 2001, Curr Opin Ophthalmol 12, 143-148; Seet et al. 2011, Mol. Med. 17, 557-567) and has successfully shown the beneficial effect of fibrotic modulators in vivo (Mead et al. 2003, Invest. Ophthalmol. Vis. Sci. 44, 3394-3401; Wong et al. 2003 Invest. Ophthalmol. Vis. Sci. 44, 1097-1103; Wong et al. 2005, Invest. Ophthalmol. Vis. Sci. 46, 2018-2022).
  • the conjunctiva was dissected to expose the underlying sclera, after which an incision was made through the sclera into the anterior chamber of the eye using a 30-gauge needle. The created fistula allowed aqueous humor to exit into and underneath the conjunctiva. The dissected conjunctiva was then secured and closed at the limbus by a 10-0 (0.2 metric) Ethilon black monofilament nylon scleral suture. Fucithalmic ointment was instilled at the end of the procedure.
  • the surgery was performed under anaesthesia by intraperitoneal injection of a 0.1 ml ketamine/xylazine mixture, as well as topical application of one drop per eye of 1% xylocaine. Fucithalmic ointment was instilled post-surgery to prevent infection. Surgery was performed with 70% propyl alcohol sterilized surgical scissors and forceps and sterile needles.
  • mice were euthanized on day 7 post-surgery for analyses.
  • eyes from mice will be harvested by enucleation and then sectioned. Maturation of collagen fibres was evaluated with using the picro-sirius red/polarization light technique (Szendroi et al. 1984, Acta Morphol Hung 32, 47-55); orange-red indicated mature collagen, and yellow/green indicated newly formed immature collagen.
  • FIGS. 38A and 38B The results of the experiment are shown in FIGS. 38A and 38B .
  • IL-11RA ⁇ / ⁇ mice were found to have a reduced fibrotic response in eye tissue as compared to IL-11RA+/+ mice.
  • IL-11RA knockout on fibrosis is also analysed in mouse models of fibrosis for other tissues, such as the liver, bowel, and is also analysed in a model relevant to multiorgan (i.e. systemic) fibrosis.
  • the fibrotic response is measured and compared between the IL-11RA ⁇ / ⁇ mice and IL-11RA+/+ mice.
  • IL-11RA ⁇ / ⁇ mice have a reduced fibrotic response as compared to IL-11RA+/+ mice, as evidenced by reduced expression of markers of fibrosis.
  • the canonical mode of action of IL-11 is thought to be regulation of RNA expression via STAT3-mediated transcription (Zhu et al., 2015 PLoS ONE 10, e0126296), and also through activation of ERK.
  • STAT3 activation is observed following stimulation with IL-11
  • fibroblasts are incubated with TGF ⁇ 1
  • only activation of the canonical SMACK pathway and ERK pathways is seen, and activation of STAT3 is not observed, even in spite of the fact that IL-11 is secreted in response to TGF ⁇ 1.
  • Only ERK activation is common to both TGF ⁇ 1 and IL-11 signal transduction.
  • TGF ⁇ 1 Block-talk between TGF ⁇ 1 and IL-6 signalling has previously been described, wherein TGF ⁇ 1 blocks the activation of STAT3 by IL-6 (Walla et al., 2003 FASEB J. 17, 2130-2132). Given the dose relationship between IL-6 and IL-11, similar cross-talk may be observed for IL-11 mediated signalling.
  • RNA-seq analysis investigated by RNA-seq analysis whether regulation of RNA abundance was the underlying mechanism for the increased expression of fibrosis marker proteins in response to IL-11, which would suggest STAT3 as the underlying signalling pathway for IL-11 mediated profibrotic processes.
  • Fibroblasts were incubated for 24 hours either without stimulus, or in the presence of TGF ⁇ 1, IL-11 or TGF ⁇ 1 and IL-11, The results are shown in FIG. 14A .
  • TGF ⁇ 1 induced the expression of collagen, ACTA2 ( ⁇ SMA) and other fibrosis marker at the RNA level.
  • IL-11 did not regulate the expression of these genes, but a different set of genes.
  • TGF ⁇ 1 increases IL-11 secretion
  • the target genes of IL-11 are not regulated when both TGF ⁇ 1 and IL-11 are present. This suggests that TGF ⁇ 1 upregulates IL-11 and simultaneously blocks the canonical IL-11-driven regulation of RNA expression via STAT3, similar to what is known about the interaction of TGF ⁇ 1 and IL-6 pathways (Walia et al., 2003 FASEB J. 17, 2130-2132).
  • RNA expression differences induced by TGF ⁇ 1 are dependent on IL-11 signalling, by analysing changes in RNA expression in fibroblasts obtained from IL-11RA ⁇ / ⁇ mice as compared to IL-11RA+4 mice.
  • RNA expression regulated by TGF ⁇ 1 is still observed when IL-11RA knockout cells were stimulated with TGF ⁇ 1, and RNA levels of ⁇ SMA, collagen etc. were still upregulated in the absence of IL-11 signalling (in IL-11RA ⁇ / ⁇ fibroblasts).
  • IL-11 and the anti-fibrotic effect of IL-11 inhibition was investigated in vitro, reduced expression of markers of fibrosis was only observed at the protein level, not at the transcriptional level as determined by qPCR.
  • non-canonical pathways e.g. ERK signal transduction
  • TGF ⁇ 1 TGF ⁇ 1
  • IL-11 a post-transcriptional regulator which is essential for fibrosis.
  • Fully human anti-human IL-11 antibodies were developed via phage display.
  • Recombinant human IL-11 (Cat. No. Z03108-1) and recombinant murine IL-11 (Cat. No. Z03052-1) were obtained from GenScript (NJ, USA). Recombinant human IL-11 was expressed in CHO cells, both as an Fc-tagged version and a tag-free version. Tag-free murine IL-11 was expressed in HEK293 cells.
  • IL-11 bioactivity of recombinant human IL-11 and mouse IL-11 was confirmed by in vitro analysis using primary fibroblast cell cultures.
  • Recombinant, biotinylated human IL-11 and murine IL-11 were also prepared by biotinylation of the recombinant human IL-11 and murine IL-11 molecules, according to standard methods.
  • Antibodies capable of binding to both human IL-11 and murine IL-11 were identified by phage display using a human nave library by panning using biotinylated and non-biotinylated recombinant human and murine IL-11, based on 16 different panning strategies as summarised in FIG. 21 .
  • the phage display identified 175 scFv binders, as ‘first hits’. Sequence analysis of the CDR sequences from these 175 scFv identified 86 unique scFv.
  • the soluble scFv were produced by recombinant expression in E. coli , and analysed for their ability to bind to human IL-11 and murine IL-11 by ELISA. Briefly, the respective antigen was coated to wells of an ELISA plate, the cell culture supernatant containing the respective scFv was added at a 1:2 dilution, and binding was detected.
  • the antibody clone designations are shown in FIG. 23 .
  • the amino acid sequence information for the antibodies is shown in FIGS. 15 to 20 .
  • VH and VL sequences of the antibodies were cloned into expression vectors for the generation of scFv-Fc (human IgG1) antibodies.
  • the vectors were transiently expressed in mammalian cells cultured in serum-free media, and isolated by protein A purification.
  • Example 5 The antibodies described in Example 5 were analysed in in vitro assays for their ability to
  • cardiac atrial human fibroblasts were cultured in wells of 96-well plates in the presence of TGF ⁇ 1 (5 ng/ml) for 24 hours, in the presence or absence of the anti-IL-11 antibodies.
  • TGF ⁇ 1 promotes the expression of IL-11, which in turn drives the transition of quiescent fibroblasts to activated, ⁇ SMA-positive fibroblasts. It has previously been shown that neutralising IL-11 prevents TGF ⁇ 1-induced transition to activated, ⁇ SMA-positive fibroblasts.
  • Anti-IL-11 antibodies (2 ⁇ g/ml) were added to fibroblast cultures that were stimulated with TGF ⁇ 1, and at the end of the 24 hour culture period, the percentage of ⁇ SMA-positive fibroblasts was determined. The percentages were normalised based on the percentage of ⁇ SMA-positive fibroblasts observed in cultures of fibroblasts which had not been stimulated with TGF ⁇ 1.
  • FIGS. 24A, 24B and 27 The results of the experiments are shown in FIGS. 24A, 24B and 27 . 28 of the antibodies were demonstrated to be capable of neutralising signalling mediated by human IL-11.
  • YU45-C11/A10 (#6), YU45-G1 (#11), YU45-E3 (#16), YU45-F8 (#18), YU45-F9 (#21), YU45-H10 (#22), YU45-F2 (#24), YU45-H3 (#25), YU45-G7 (#33), YU45-B6 (#36), YU45-C1 (#42), YU46-B6 (#47), YU46-E3 (#50), YU46-G8 (#54) and YU46-D3 (#56).
  • FIGS. 25 and 27 The results of the experiments are shown in FIGS. 25 and 27 .
  • the antibodies were demonstrated to be capable of neutralising signalling mediated by mouse IL-11.
  • YU33-B4/YU45-G2/A3 (#3), YU45-H11/D12 (#9), YU45-G1 (#11), YU45-D2/H2/C7/F3/C9/E1/E9/C10/G3/H9/C5/A2/A5 (#14), YU45-B3 (#15), YU45-F8 (#18), YU45-H10 (#22), YU46-A10 (#23), YU45-A8/C6 (#27), YU45-D9/D3 (#31), YU45-B6 (#36), YU45-C1 (#42), YU46-A8 (#45), YU46-C1 (#48), YU46-H8
  • the ability of the human antibodies to inhibit mouse IL-11-mediated signalling was also investigated using mouse skin fibroblasts.
  • the results of the experiments are shown in FIG. 27 .
  • the antibodies were demonstrated to be capable of neutralising signalling mediated by mouse IL-11.
  • Trans signalling is recognised as a major aspect of IL-6 signalling, where a complex of IL-6 and soluble IL-6R ⁇ can activate cells that express gp130, but lack the IL-6 receptor (Hunter and Jones, 2015 Nature Immunology 16, 448-457).
  • antibodies which are capable of inhibiting both classical IL-11 mediated signalling and IL-11 trans signalling by IL-11:IL-11R ⁇ complex are able to inhibit all known modes of IL-11/IL-11R signalling.
  • the IL-11:IL-11R ⁇ fusion protein (hereafter referred to as hyper IL-11) consists of the extracellular domain of the IL-11 receptor alpha (IL-11R ⁇ ) linked to IL-11.
  • Hyper IL-11 was found to be a more potent activator of human fibroblasts than recombinant IL-11 protein. Briefly, in two separate experiments human fibroblasts were cultured without stimulation (Baseline), in the presence of different amounts of hyper IL-11 (0.008 ng/ml, 0.04 ng/ml, 0.2 ng/ml, 1 ng/ml and 5 ng/ml), or 5 ng/ml recombinant human IL-11 obtained from a commercial source, and fibroblast activation was analysed by determining the percentage of ⁇ SMA-positive cells as described herein. The results are shown in ( FIGS. 42A and 42B ). Hyper-IL-11 activated fibroblasts in a dose-dependent fashion, and was a more potent activator than IL-11.
  • the IL-11:IL-11R ⁇ fusion protein was prepared as follows:
  • Fibroblasts cultured in vitro and stimulated with hyper IL-11 were shown to upregulate IL-11 protein expression, as determined by ELISA ( FIG. 43 ).
  • an increase in IL-11RNA level was not detected in response to stimulation with hyper IL-11.
  • hyper IL-11 seems to upregulate IL-11 expression only post-transcriptionally, at the protein level.
  • Human atrial fibroblasts derived from 3 individuals were incubated for 24 h with hyper IL-11 (0.2 ng/ml) in the presence of neutralising anti-IL-11 antibody or isotype control antibody. Following incubation, cells were stained for ⁇ SMA to determine the fraction of myofibroblasts.
  • the results of the experiments are shown in FIGS. 26 and 27 .
  • the antibodies were demonstrated to be capable of neutralising signalling mediated by hyper IL-11 (i.e. IL-11 trans signalling).
  • Clone YU33-B4/YU45-G2/A3 (#3) neutralised IL-11 trans signalling by hyper IL-11 to a greater extent than the commercially available mouse anti-IL-11 antibody (industry standard).
  • Clones YU33-B4/YU45-G2/A3 (#3), YU45-E3 (#16), YU45-F2 (#24), YU45-F5 (#39), YU46-A8 (#45) and YU46-G8 (#54) were identified as particularly promising candidates, showing good ability to inhibit signalling by both human and mouse IL-11, and good inhibition of IL-11 trans signalling.
  • the human anti-human IL-11 antibodies were analysed for their affinity of binding to human IL-11 by ELISA assay.
  • Recombinant human IL-11 was obtained from Genscript and Horseradish peroxidase (HRP)-conjugated anti-human IgG (Fc-specific) antibody was obtained from Sigma.
  • Corning 96-well ELISA plates were obtained from Sigma.
  • Pierce 3,3′,5,5′-tetramethylbenzidine (TMB) ELISA substrate kit was obtained from Life Technologies (0.4 g/mL TMB solution, 0.02% hydrogen peroxide in citric acid buffer).
  • Bovine serum albumin and sulphuric acid was obtained from Sigma. Wash buffer comprised 0.05% Tween-20 in phosphate buffered saline (PBS-T). ScFv-Fc antibodies were generated as described in Example 5.
  • Purified mouse and human IgG controls were purchased from Life Technologies. Tecan Infinite 200 PRO NanoQuant was used to measure absorbance.
  • Detection of antigen-antibody binding was performed with 15.625 ng/mL of HRP-conjugated anti-human IgG (Fc-specific) antibody. Following 2 hours of incubation with the detection antibody, 100 ⁇ l of TMB substrate was added for 15 mins and chromogenic reaction stopped with 100 ⁇ l of 2 M H 2 SO 4 . Absorbance reading was measured at 450 nm with reference wavelength correction at 570 nm. Data were fitted with Graph Pad Prism software with log transformation of antibody concentrations followed by non-linear regression analysis with the asymmetrical (five-parameter) logistic dose-response curve to determine individual EC50 values.
  • FIG. 28A to 28F The results of the ELISA assays are shown in FIG. 28A to 28F , and were used to determine EC 50 values for the antibodies which are shown in FIG. 29 .
  • Anti-IL-11 antibodies are demonstrated to be capable of neutralising signalling in fibroblasts derived from the various different tissues, as determined by observation of a relative decrease in the proportion of ⁇ SMA-positive fibroblasts at the end of the 24 h culture period in the presence of the anti-IL-11 antibodies as compared to culture in the absence of the antibodies.
  • Human IL-11 antibodies are affinity-matured by light chain shuffling to obtain antibodies having improved affinity for IL-11.
  • Chain shuffling to improve antibody affinity is a well-known technique in the field of antibody technology, and is described in detail in Marks, Antibody Affinity Maturation by Chain Shuffling, Antibody Engineering Methods and Protocols, Humana Press (2004) Vol. 248, pp 327-343, incorporated by reference herein. In particular, Light chain shuffling is described in detail at sections 3.1 and 3.2 thereof.
  • the heavy chain variable regions of the human anti-human IL-11 antibodies are combined with a repertoire of light chain variable region partners to identify new VLNH combinations having high affinity for IL-11.
  • FIG. 30 A schematic representation of light chain shuffling is shown in FIG. 30 . Briefly, nucleic acid encoding the VH domain for an antibody is cloned into a phage display vector comprising a repertoire of VL chains, and scFv comprising new VH/VL combinations are analysed for binding to human IL-11 by ELISA.
  • the scFv having VH/VL combinations displaying the strongest binding affinity for IL-11 are then analysed for cross-reactivity against murine IL-11.
  • VH/VL sequences of the scFv are then cloned into expression vectors for the generation of scFv-Fc (human IgG1) antibodies, the vectors are transiently expressed in mammalian cells cultured in serum-free media, and isolated by protein A purification.
  • Mouse monoclonal antibodies directed against human IL-11 protein were also generated, as follows.
  • cDNA encoding the amino acid for human IL-11 was cloned into expression plasmids (Aldevron GmbH, Freiburg, Germany).
  • mice were immunised by intradermal application of DNA-coated gold-particles using a hand-held device for particle-bombardment (“gene gun”). Serum samples were collected from mice after a series of immunisations, and tested in flow cytometry on HEK cells which had been transiently transfected with human IL-11 expression plasmids (cell surface expression of human IL-11 by transiently transfected HEK cells was confirmed with anti-tag antibodies recognising a tag added to the N-terminus of the IL-11 protein).
  • Antibody-producing cells were isolated from the mice and fused with mouse myeloma cells (Ag8) according to standard procedures.
  • Hybridomas producing antibodies specific for IL-11 were identified by screening for ability to bind to IL-11 expressing HEK cells by flow cytometry.
  • RNA protection agent RNAlater, cat. #AM7020 by ThermoFisher Scientific
  • sequencing of the variable domains of the antibodies were prepared using an RNA protection agent (RNAlater, cat. #AM7020 by ThermoFisher Scientific) and further processed for sequencing of the variable domains of the antibodies.
  • mice monoclonal anti-human IL-11 antibodies were prepared ( FIG. 31 ).
  • FIGS. 32 and 35 The results of the Experiments are shown in FIGS. 32 and 35 .
  • the antibodies were demonstrated to be capable of neutralising signalling mediated by human IL-11.
  • a commercial monoclonal mouse anti-IL-11 antibody (Monoclonal Mouse IgG2A; Clone #22626; Catalog No. MAB218; R&D Systems, MN, USA) was also analysed for ability to inhibit signalling by human IL-11 in the experiments. This antibody was found to be able to reduce the percentage of activated fibroblasts to 0.89 times.
  • Clone A7 (BSN-3C11) was found to neutralise signalling by human IL-11 to a greater extent than the commercially available mouse anti-IL-11 antibody (industry standard).
  • the ability of the murine monoclonal anti-human IL-11 antibodies to inhibit signalling mediated by murine IL-11 was investigated using the same assay as described in Example 6.2 above, but using mouse atrial fibroblasts instead of mouse dermal fibroblasts.
  • FIGS. 33 and 35 The results of the Experiments are shown in FIGS. 33 and 35 .
  • the antibodies were demonstrated to be capable of neutralising signalling mediated by murine IL-11.
  • mice anti-IL-11 antibodies The ability of the mouse anti-IL-11 antibodies to inhibit signalling mediated by hyper IL-11 was investigated.
  • Human atrial fibroblasts were incubated for 24 h with hyper IL-11 (0.2 ng/ml) in the presence of anti-IL-11 antibodies (2 ⁇ g/ml) or isotype control antibody. Following incubation, cell culture supernatant was analysed for MMP2. Stimulation with hyper IL-11 results in an increase in the secretion of MMP2 as compared to non-stimulated cultures.
  • mice anti-IL-11 antibodies were found to be capable of neutralising signalling mediated by hyper IL-11 (i.e. IL-11 trans signalling), and several were found to be capable of inhibiting trans signalling to a greater extent than the commercial monoclonal mouse anti-IL-11 antibody (Monoclonal Mouse IgG2A; Clone #22626; Catalog No. MAB218; R&D Systems, MN, USA): BSN-2G6 (A5), BSN-3C6 (A6), BSN-5B8 (A9) and BSN-7D4 (A12).
  • hyper IL-11 i.e. IL-11 trans signalling
  • BSN-2G6 A5
  • BSN-3C6 A6
  • BSN-5B8 A9
  • BSN-7D4 A12
  • Clone BSN-3C6 (A6) was identified as a particularly promising candidate for further development (highlighted in FIG. 35 ), showing good ability to inhibit both human IL-11 and mouse IL-11 mediated signalling, and good inhibition of IL-11 trans signalling.
  • the mouse hybridomas producing anti-human IL-11 antibodies were sub-cloned, and cell culture supernatant from the subcloned hybridomas was analysed by “mix-and-measure” iQue assay for (i) ability to bind to human IL-11, and (ii) cross reactivity for antigen other than IL-11.
  • control cells not expressing IL-11 at the cell surface
  • unlabelled target cells expressing human IL-11 at their surface following transient transfection with a plasmid encoding a FLAG-tagged human IL-11
  • cell culture supernatant containing mouse-anti-IL-11 antibodies
  • secondary detection antibodies fluorescently-labelled anti-mouse IgG antibody
  • the cells were then analysed using the HTFC Screening System (iQue) for the two labels (i.e. the cell label and the label on the secondary antibody). Detection of the secondary antibody on the unlabelled, IL-11 expressing cells indicated ability of the mouse-anti-IL-11 antibodies to bind to IL-11. Detection of the secondary antibody on the labelled, control cells indicated cross-reactivity of the mouse-anti-IL-11 antibodies for target other than IL-11.
  • labelled and unlabelled cells were incubated with a mouse anti-FLAG tag antibody as the primary antibody.
  • FIGS. 36A and 36B The results are shown in FIGS. 36A and 36B .
  • Clones BSN-2G6, BSN-5B8 and BSN-7F9 displayed some binding to cells not expressing IL-11, and so may have cross-reactivity for target(s) other than IL-11.
  • Antibody produced by subcloned BSN-3C11 was found not to bind to human IL-11.
  • Mouse/human chimeric and humanised versions of the mouse monoclonal anti-human IL-11 antibodies of Example 8 are prepared according to standard methods.
  • Mouse/human chimeric antibodies are prepared from the mouse monoclonal anti-human IL-11 antibodies as described in Human Monoclonal Antibodies: Methods and Protocols, Michael Steinitz (Editor), Methods in Molecular Biology 1060, Springer Protocols, Humana Press (2014), in Chapter 8 thereof.
  • the DNA sequences encoding the VH and VL of hybridomas producing the mouse anti-human IL-11 antibodies are determined, and combined with DNA sequence encoding human immunoglobulin constant regions to produce a mouse/human chimeric antibody sequence, from which a chimeric mouse/human antibody is expressed in mammalian cells.
  • Humanised antibodies are prepared from the mouse monoclonal anti-human IL-11 antibodies as described in Human Monoclonal Antibodies: Methods and Protocols, Michael Steinitz (Editor), Methods in Molecular Biology 1060, Springer Protocols, Humana Press (2014), in Chapter 7 thereof, in particular at section 3.1 of Chapter 7 entitled ‘Antibody Humanization’.
  • DNA sequences encoding the VH and VL of hybridomas producing the mouse anti-human IL-11 antibodies are determined, and inserted into DNA sequence encoding human antibody variable region framework regions and immunoglobulin constant regions, to produce a humanised antibody sequence, from which a humanised antibody is expressed in mammalian cells.
  • the antibodies described above are subjected to further biochemical analysis.
  • the antibodies are analysed by BIAcore, Biolayer interferometry (BLI) and MicroScale Thermophoresis (MST) analysis to determine the affinity of binding to human IL-11 and mouse IL-11.
  • SEC size exclusion chromatography
  • Hydophobicity of the antibodies is analysed by Hydrophobic interaction chromatography (HIC) as described in Haverick et al., MAbs. 2014 July-August; 6(4):852-8.
  • HIC Hydrophobic interaction chromatography
  • the melting temperature of the antibodies is analysed by Differential scanning fluorimetry (DSF) as described in Menzen and Friess, J Pharm Sci. 2013 February; 102(2):415-28.
  • DSF Differential scanning fluorimetry
  • Example 12 Inhibition of Fibrosis In Vivo Using Anti-IL-11 Antibodies
  • mice used in the experiments are wildtype (i.e. IL-11RA+/+) mice.
  • a pump is implanted, and mice are treated with AngII (2 mg/kg/day) for 28 days.
  • Neutralising anti-IL-11 antibodies, or control antibodies are administered to different groups of mice by intravenous injection.
  • collagen content is assessed in the atria of the mice using a calorimetric hydroxyproline-based assay kit, and the level of RNA expression of the markers or fibrosis Col1A2, ⁇ SMA (ACTA2) and fibronectin (Fn1) were analysed by qPCR.
  • mice treated with neutralising anti-IL-11 antibodies have a reduced fibrotic response in heart tissue as compared to mice treated with control antibodies, as evidenced by reduced expression of markers of fibrosis.
  • a mouse model for kidney fibrosis is established, in which fibrosis is induced by intraperitoneal injection of folic acid (180 mg/kg) in vehicle (0.3M NaHCO 3 ); control mice were administered vehicle alone.
  • Neutralising anti-IL-11 antibodies, or control antibodies are administered to different groups of mice by intravenous injection. Kidneys are removed at day 28, weighed and either fixed in 10% neutral-buffered formalin for Masson's trichrome and Sirius staining or snap-frozen for collagen assay, RNA, and protein studies.
  • RNA is extracted from the snap-frozen kidney using Trizol reagent (Invitrogen) and Qiagen TissueLyzer method followed by RNeasy column (Qiagen) purification.
  • the cDNA is prepared using iScriptTM cDNA synthesis kit, in which each reaction contained 1 ⁇ g of total RNA, as per the manufacturer's instructions.
  • Quantitative RT-PCR gene expression analysis is performed on triplicate samples with either TaqMan (Applied Biosystems) or fast SYBR green (Qiagen) technology using StepOnePlusTM (Applied Biosystem) over 40 cycles. Expression data are normalized to GAPDH mRNA expression level and the 2- ⁇ Ct method is used to calculate the fold-change.
  • the snap-frozen kidneys are subjected to acid hydrolysis by heating in 6M HCl at a concentration of 50 mg/ml (95° C., 20 hours).
  • the amount of total collagen in the hydrolysate is quantified based on the colorimetric detection of hydroxyproline using Quickzyme Total Collagen assay kit (Quickzyme Biosciences) as per the manufacturer's instructions.
  • mice treated with neutralising anti-IL-11 antibodies have a reduced fibrotic response in kidney tissue as compared to mice treated with control antibodies, as evidenced by reduced expression of markers of fibrosis.
  • mice are treated by intratracheal administration of bleomycin on day 0 to establish a fibrotic response in the lung (pulmonary fibrosis).
  • Neutralising anti-IL-11 antibodies, or control antibodies are administered to different groups of mice by intravenous injection. Mice are sacrificed at day 21, and analysed for differences in fibrosis markers.
  • mice treated with neutralising anti-IL-11 antibodies have a reduced fibrotic response in lung tissue as compared to mice treated with control antibodies, as evidenced by reduced expression of markers of fibrosis.
  • mice are treated by subcutaneous administration of bleomycin on day 0 to establish a fibrotic response in the skin.
  • Neutralising anti-IL-11 antibodies, or control antibodies are administered to different groups of mice by intravenous injection. Mice are sacrificed at day 21, and analysed for differences in fibrosis markers.
  • mice treated with neutralising anti-IL-11 antibodies have a reduced fibrotic response in skin tissue as compared to mice treated with control antibodies, as evidenced by reduced expression of markers of fibrosis.
  • mice undergo trabeculectomy procedure as described in Example 3.6 above to initiate a wound healing response in the eye.
  • Neutralising anti-IL-11 antibodies, or control antibodies are administered to different groups of mice by intravenous injection, and fibrosis is monitored in the eye tissue.
  • mice treated with neutralising anti-IL-11 antibodies have a reduced fibrotic response in eye tissue as compared to mice treated with control antibodies, as evidenced by reduced expression of markers of fibrosis.
  • the effect of treatment with neutralising anti-IL-11 antibodies on fibrosis is also analysed in mouse models of fibrosis for other tissues, such as the liver, kidney, bowel, and is also analysed in a model relevant to multiorgan (i.e. systemic) fibrosis.
  • mice treated with neutralising anti-IL-11 antibodies have a reduced fibrotic response as compared to mice treated with control antibodies, as evidenced by reduced expression of markers of fibrosis.
  • mice are treated by administration of neutralising anti-IL-11 antibodies, or control antibodies, and the development/progression of cancer is monitored.
  • ATD age-related macular degeneration
  • Neutralising anti-IL-11 antibody is administered to subjects having wet AMD.
  • subjects are administered with VEGF antagonist therapy (e.g. ranibizumab, bevacizumab, pegaptanib, brolucizumab or aflibercept), PDGF antagonist therapy (e.g. pegpleranib), or are treated by laser coagulation therapy in addition to treatment with anti-IL-11 antibody.
  • VEGF antagonist therapy e.g. ranibizumab, bevacizumab, pegaptanib, brolucizumab or aflibercept
  • PDGF antagonist therapy e.g. pegpleranib
  • a reduction in wet AMD pathology and/or improvement in the symptoms of wet AMD is observed in subjects treated with anti-IL-11 antibody as compared to subjects not treated with anti-IL-11 antibody.
  • Light chain shuffling was performed as represented schematically in FIG. 30 .
  • the heavy chains of the following IL-11-binding antibody clones were used for light chain shuffling: YU45-E03, YU45-F02, YU45-F05, YU45-G02, YU46-A08, YU46-G08.
  • Variable regions of the heavy chains were amplified by PCR, and the resulting amplicons were pooled and cloned into phagemid vectors (phagemids) each containing a specific VL chain, and representing nave lambda and kappa light chain library repertoires.
  • the VH and VL containing phagemids were used to produce a new library of antibody-phages, which was used to select clones displaying binding to IL-11 under stringent conditions (i.e. antigen limitation, large number washing steps).
  • Antibodies capable of binding to both human IL-11 and murine IL-11 were identified by phage display by panning using biotinylated and non-biotinylated recombinant human and murine IL-11, based on the panning strategy shown in FIG. 52 .
  • the analysis identified 66 cross-reactive antibodies ( FIG. 53 ). Sequence analysis identified 64 unique antibody clones, the amino acid sequences of which are shown in FIG. 50 , and the nucleotide sequences of which are shown in FIG. 51 .
  • the 64 antibody clones were analysed for binding signal to human IL-11 and murine IL-11 in an ELISA assay. The results are shown in FIGS. 54A and 54B .
  • the light chain shuffled anti-IL-11 antibodies were analysed to determine the EC50 for binding to human IL-11 by ELISA according to standard methods. Briefly, wells of microtiter plates were coated with recombinant human IL-11 (100 ng/well), scFv-Fc comprising the VH and VL domains of the clones were added in a dilution series and antibody binding was detected using a polyclonal antibody detection system.
  • cardiac atrial human fibroblasts were cultured in wells of 96-well plates in the presence of TGF ⁇ 1 (5 ng/ml) for 24 hours, in the presence of anti-IL-11 antibodies in scFv-human IgG1-Fc format, or in the presence of human IgG1 isotype control antibody, at a final concentration of 2 mg/ml.
  • TGF ⁇ 1 5 ng/ml
  • anti-IL-11 antibodies in scFv-human IgG1-Fc format
  • human IgG1 isotype control antibody at a final concentration of 2 mg/ml.
  • Levels of the pro-fibrotic marker MMP2 in the cell culture supernatant were then measured by ELISA. Basal MMP2 secretion by the cells in culture was measured by culture in the absence of TGF ⁇ 1, in the presence of human IgG1 isotype control (2 mg/ml).
  • FIGS. 56A and 56B The results of two separate experiments are shown in FIGS. 56A and 56B .
  • Horizontal lines in the bar charts represent the basal MMP2 secretion by cardiac atrial human fibroblasts cultured for 24 hours in the presence of human IgG1 isotype control antibody in the absence of TGF ⁇ 1 stimulation (‘NEG’ in FIGS. 56A and 56B ), and MMP2 secretion by cardiac atrial human fibroblasts cultured for 24 hours in the presence of 5 ng/ml TGF ⁇ and the human IgG1 isotype control antibody (‘POS’ in FIGS. 56A and 56B ).
  • the light chain shuffled anti-IL-11 antibodies were shown to be able to bind to human IL-11, and to inhibit IL-11 mediated signalling.
  • mice of similar weight had kidney fibrosis induced by intraperitoneal (i.p.) injection of folic acid (180 mg kg ⁇ 1 ) in vehicle (0.3 M NaHCO 3 ); control mice were administered vehicle alone.
  • Anti-IL11 antibody clone BSN-3C6 was administered one day after folic acid treatment and then 3 times per week at a dose of 20 mg/kg. Mice were euthanized 28 days post-injection.
  • the mouse plasma levels of urea and creatinine were quantified using urea assay kit (ab83362, Abcam) and creatinine assay kit (ab65340, Abcam), respectively according to the manufacturer's instructions.
  • the amount of total collagen in the kidney was quantified on the basis of colourimetric detection of hydroxyproline using a Quickzyme Total Collagen assay kit (Quickzyme Biosciences). All colourimetric assays were performed according to the manufacturer's instructions.
  • Tissues were paraffin-embedded, and kidneys were sectioned at 3 ⁇ m.
  • tissues were fixed for 24 h, at room temperature in 10% neutral-buffered formalin (Sigma-Aldrich), dehydrated and embedded in paraffin.
  • For cryosections freshly dissected organs were embedded with Tissue-Tek Optimal Cutting Temperature compound (VWR International). Cryomoulds were then frozen in a metal beaker with isopentane cooled in liquid nitrogen and sections were stored in 80° C. Total collagen was stained with Masson's trichrome stain kit (HT15, Sigma-Aldrich) according to the manufacturer's instructions.
  • HT15 Masson's trichrome stain kit
  • FIGS. 58A and 58B show that mice treated with anti-IL11 antibody were found to have significantly reduced staining for collagen, indicating that anti-IL-11 antibody treatment had inhibited kidney fibrosis.
  • FIG. 59 shows that the urinary albumin/creatine ratio was significantly reduced by treatment with anti-IL11 antibody, indicating a reduced level of kidney damage in mice treated with anti-IL-11 antibody.
  • FIG. 60 shows that treatment with the anti-IL-11 antibody inhibited folic acid-induced kidney fibrosis in a dose-dependent fashion.
  • mice were treated by sham operation or ureteric obstruction of one ureter.
  • Mice received IgG, anti-IL-11 antibody clone BSN-3C6 (20 mg/kg; on surgical days ⁇ 1, 1, 3, 5) and injured kidneys NUM or contralateral uninjured kidneys (Con) were harvested on day 7 post surgery.
  • Tubular injury score 0, none; 1, minimal; 2, mild; 3, moderate; 4, severe.
  • FIGS. 61A and 61B show that treatment with anti-IL-11 antibody reduced tubular damage in a mouse model of acute renal injury.
  • Example 18 IL-11 and Liver Fibrosis
  • Protein expression of IL-11 in healthy and diseased livers was confirmed by western blots in matched samples of human livers. Matched frozen liver samples were prepared for western blotting and levels of IL11 determined using Human IL-11 Antibody Monoclonal Mouse IgG2A Clone #22626, catalog number MAB218 from R&D Systems. Film images were generated.
  • the results are shown in FIG. 63 .
  • the profibrogenic stimuli induced upregulation of IL-11 protein expression, and ALK5 inhibitor was found to inhibit TGF ⁇ 1 receptor signalling, which reduced the expression of IL-11 protein down to control levels.
  • Diabetic mice (db/db; deficient for the leptin receptor) were maintained for 8 weeks on a normal chow diet or on a NASH-inducing (methionine/choline deficient (MCD)) diet.
  • MCD methionine/choline deficient
  • FIGS. 64A and 64B The results are shown in FIGS. 64A and 64B .
  • Inhibition of IL-11 mediated signalling by anti-IL-11 antibody treatment improved liver histology in a mouse model of nonalcoholic steatohepatitis ( FIG. 64A ) as evidenced by partial restoration of liver morphology and texture in anti-IL-11 antibody-treated animals on NASH diet as compared to untreated animals on NASH diet.
  • Livers from mice treated with anti-IL-11 antibody on NASH diet were also found to have reduced collagen content as compared to untreated animals on NASH diet ( FIG. 64B ).
  • anti-fibrotic effect of anti-IL-11 antibody treatment was assessed in a mouse model of retinal fibrosis in which Bruch's membrane is disrupted, as described in Caballero et al., Exp Eye Res. (2009) March; 88(3):367-77.
  • mice were subjected to laser-induced retinal damage (4 burns per retina) and were then treated by intraocular administration of antibodies (0.5 ⁇ g of either IgG control or anti-IL11 antibody clone BSN-3C6) on days 1, 7, 14 and 21. Eyes were harvested for histological analyses on day 28. The area of fibrosis at burn sites was measured using Masson's Trichrome staining, blinded to treatment.
  • antibodies 0.5 ⁇ g of either IgG control or anti-IL11 antibody clone BSN-3C6
  • FIGS. 65A and 65B The results are shown in FIGS. 65A and 65B .
  • the area of fibrosis was significantly greater in control IgG-treated mice as compared to anti-IL11 antibody treated mice.
  • the anti-fibrotic effect of anti-IL-11 antibody treatment was analysed in a mouse model of skin fibrosis stablished by subcutaneous injection of bleomycin (BLM, Sigma B2434, 50 ⁇ g/day).
  • FIGS. 66B and 66C show that dermal thickness was significantly reduced in mice treated with neutralising anti-IL-11 antibody as compared to control IgG-treated mice. Increased collagen staining can also be seen for the control IgG-treated group ( FIG. 66B , middle panel).
  • Example 21 Inhibition of Heart Fibrosis Using Anti-IL-11 Antibodies
  • TAC transverse aortic constriction
  • mice were euthanized at 2 weeks post-TAC for histological and molecular assessment.
  • Anti-IL-11 antibody clone BSN-3C6 or control IgG antibody were administered intraperitoneally 3 times per week at a dose of 20 mg/kg. After two weeks hearts were harvested and assessed for fibrosis extent using Masson's Trichrome stain kit (HT15, Sigma-Aldrich), in accordance with the manufacturer's instructions.
  • mice treated with neutralising anti-IL-11 antibody were found to have reduced levels of fibrosis in the epicardium, endocardium and in perivascular regions as compared to mice treated with IgG control antibody.

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109503712A (zh) * 2018-12-12 2019-03-22 广州市第八人民医院 一种单克隆抗体ZKns2E11及其应用
US10822405B2 (en) 2015-12-16 2020-11-03 Singapore Health Services Pte Ltd. Treatment of fibrosis with IL-11 receptor alpha antibody
US20210230266A1 (en) * 2018-06-13 2021-07-29 Singapore Health Services Pte Ltd. Il-11 antibodies
US11078269B2 (en) 2016-12-16 2021-08-03 Singapore Health Services Pte Ltd IL-11Rα antibodies
US11078268B2 (en) 2016-12-16 2021-08-03 Singapore Health Services Pte Ltd IL-11 antibodies
US11319368B2 (en) 2019-01-21 2022-05-03 Singapore Health Services Pte Ltd. Treatment of hepatotoxicity with IL-11 antibody

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201716733D0 (en) * 2017-10-12 2017-11-29 Nat Univ Singapore Treatment of SMC mediated disease
GB201902419D0 (en) 2019-02-22 2019-04-10 Singapore Health Serv Pte Ltd Treatment of kidney injury
GB201906291D0 (en) 2019-05-03 2019-06-19 Singapore Health Serv Pte Ltd Treatment and prevention of metabolic diseases
PH12021552763A1 (en) * 2019-05-03 2022-07-04 Nat Univ Singapore Treatment and prevention of metabolic diseases
GB202009292D0 (en) 2020-06-18 2020-08-05 Singapore Health Serv Pte Ltd Treatment and prevention of disease caused by type IV collagen dysfunction
CN113651888B (zh) * 2020-08-13 2022-08-16 广东东阳光药业有限公司 Il-11的抗体及其应用
GB202017244D0 (en) 2020-10-30 2020-12-16 Nat Univ Singapore Methods to extend health-span and treat age-related diseases
CN113980129B (zh) * 2021-01-15 2023-07-28 东大生物技术(苏州)有限公司 一组il-11单克隆抗体及其医药用途
AU2022225064A1 (en) 2021-02-26 2023-08-10 Bayer Aktiengesellschaft Inhibitors of il-11 or il-11ra for use in the treatment of abnormal uterine bleeding
EP4323401A1 (en) * 2021-04-12 2024-02-21 Syddansk Universitet Mfap4 and treatment of fibrosis
US20250092126A1 (en) 2021-07-26 2025-03-20 Boehringer Ingelheim International Gmbh Treatment and prevention of alcoholic liver disease
JP2024532709A (ja) * 2021-08-12 2024-09-10 サンシャイン・レイク・ファーマ・カンパニー・リミテッド Ｉｌ－１１ヒト化抗体及びその用途
JP2024532620A (ja) 2021-08-30 2024-09-05 ラッセン・セラピューティクス１・インコーポレイテッド 抗ＩＬ－１１Ｒα抗体
WO2023111196A1 (en) 2021-12-16 2023-06-22 Singapore Health Services Pte. Ltd. Treatment and prevention of glomerular disease
CN114384245B (zh) * 2022-03-24 2022-06-24 江苏美克医学技术有限公司 新型冠状病毒s-rbd蛋白和n蛋白双表位识别的联检试剂盒及其应用
WO2024158700A2 (en) * 2023-01-23 2024-08-02 The Regents Of The University Of California Methods and compositions for treating ewing sarcoma
WO2024226646A2 (en) * 2023-04-25 2024-10-31 Memorial Sloan-Kettering Cancer Center Dlk1 -targeting antibodies and uses thereof
WO2024225979A1 (en) 2023-04-25 2024-10-31 Vvb Bio Pte Ltd Anti il-11 antibody
CN119080927B (zh) * 2023-06-05 2025-06-24 北京东方百泰生物科技股份有限公司 一种结合il-11的抗体、其抗原结合片段及应用
WO2025024431A1 (en) * 2023-07-24 2025-01-30 Vanderbilt University Broadly reactive antibodies to influenza b viruses
CN117085137A (zh) * 2023-10-10 2023-11-21 上海交通大学医学院附属第九人民医院 Il-11抑制剂及其在抗瘢痕治疗中的应用
WO2025108343A1 (zh) * 2023-11-22 2025-05-30 上海华奥泰生物药业股份有限公司 靶向il-11的抗原结合蛋白

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69535274T2 (de) 1994-12-22 2007-06-06 Genetics Institute, LLC, Cambridge Verwendung eines menschlichen interleukin-11 rezeptors
US5679339A (en) 1995-06-27 1997-10-21 Keith; James Method of using IL-11 for treating spondyloarthropies
US6126933A (en) 1995-06-27 2000-10-03 Genetics Institute Methods of treating inflammatory bowel diseases by administering IL-11
US6540993B1 (en) 1995-06-27 2003-04-01 Wyeth Method of treating inflammatory bowel disease using a topical formulation of IL-11
GB9702944D0 (en) 1997-02-13 1997-04-02 Univ Manchester Reducing fibrosis
GB9721961D0 (en) 1997-10-16 1997-12-17 Glaxo Group Ltd Novel molecules
CA2237915A1 (en) 1998-05-19 1999-11-19 Stephen Shaughnessy Osteoporosis treatment
US6953777B2 (en) 1999-03-11 2005-10-11 Genetics Indtitute LLC Use of interleukin-11 to prevent immune-mediated cytotoxicity
AU5756100A (en) 1999-06-21 2001-01-09 Genetics Institute Inc. Treatment of fibrosis by antagonism of il-13 and il-13 receptor chains
GB0021668D0 (en) 2000-09-04 2000-10-18 Smithkline Beecham Plc New use
BR0314356A (pt) 2002-09-16 2005-07-19 Wyeth Corp Formulações de liberação retardada para administração oral de um agente terapêutico polipetìdeo e métodos utilizando as mesmas
EP1709074A4 (en) 2003-12-16 2008-06-11 Commw Scient Ind Res Org CYTOKIN BINDING DOMAINS
US20060019890A1 (en) 2004-01-15 2006-01-26 Kapoun Ann M Method for treating cardiac remodeling following myocardial injury
WO2005098041A2 (en) 2004-03-26 2005-10-20 University Of Florida Research Foundation, Inc. Detection and treatment of fibrotic disorders
EP1598364A1 (en) * 2004-05-21 2005-11-23 AGIRx Limited Chimerical soluble hyper IL-11 receptor and use thereof
EP1630232B1 (en) 2004-08-27 2008-07-02 CONARIS research institute AG Optimized nucleotide sequences encoding sgp130
WO2007067602A1 (en) 2005-12-06 2007-06-14 Wyeth Interleukin-11 compositions and methods of use
JP5191393B2 (ja) 2006-10-27 2013-05-08 国立大学法人大阪大学 インターロイキン１１の心疾患治療薬としての利用
US8182814B2 (en) 2007-10-26 2012-05-22 Csl Limited Methods of treating inflammatory airway conditions by inhibition of IL-11 activity
ES2536877T3 (es) 2007-10-26 2015-05-29 Csl Limited Muteínas de citocina
US8518888B2 (en) 2008-10-14 2013-08-27 Csl Limited Method of treatment of gastrointestinal-type cancer with antagonistic antibodies to IL-11R
KR101133769B1 (ko) 2011-04-18 2012-04-09 한국생명공학연구원 신규한 옥사졸리딘계 및 인돌계 화합물, 이의 제조방법 및 이를 포함하는 약학 조성물
SG11201505762XA (en) 2013-02-07 2015-08-28 Csl Ltd Il-11r binding proteins and uses thereof
CN105497893B (zh) 2015-12-10 2019-02-01 黑龙江省科学院高技术研究院 IL-11单克隆抗体在抑制PI3K/Akt信号通路中的应用
GB201522186D0 (en) 2015-12-16 2016-01-27 Singapore Health Services Pte Ltd And Nat University Of Singapore The Treatment of fibrosis
RU2016125115A (ru) 2016-06-23 2017-12-28 Федеральное государственное бюджетное учреждение науки Институт биологического приборостроения с опытным производством Российской Академии наук (ИБП РАН) Терапевтическое антитело, специфичное к интерлейкину-11, ингибирующее сигналирование через его рецептор, и способ его использования для лечения пациентов
KR20190096390A (ko) 2016-12-16 2019-08-19 싱가포르 헬스 서비시즈 피티이 엘티디 Il-11 항체
GB201621439D0 (en) 2016-12-16 2017-02-01 Singapore Health Services Pte Ltd And Nat Univ Of Singapore IL-11Ra Antibodies
RU2016151730A (ru) 2016-12-28 2018-06-29 Федеральное государственное бюджетное учреждение науки Институт биологического приборостроения с опытным производством Российской Академии наук (ИБП РАН) Антитела для лабораторной диагностики концентрации интерлейкина-11
GB201809699D0 (en) 2018-06-13 2018-08-01 Singapore Health Serv Pte Ltd IL-11 antibodies
GB201809700D0 (en) 2018-06-13 2018-08-01 Singapore Health Serv Pte Ltd IL-11 antibodies
CN113651888B (zh) * 2020-08-13 2022-08-16 广东东阳光药业有限公司 Il-11的抗体及其应用
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US20200199218A1 (en) 2020-06-25
AU2017378111A1 (en) 2019-06-13
US11078268B2 (en) 2021-08-03
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JP2020511414A (ja) 2020-04-16
EP3555131A2 (en) 2019-10-23
CA3045871A1 (en) 2018-06-21
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WO2018109174A2 (en) 2018-06-21
KR20190096390A (ko) 2019-08-19

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