US20160338368A1 - Fruit and vegetable preservative - Google Patents
Fruit and vegetable preservative Download PDFInfo
- Publication number
- US20160338368A1 US20160338368A1 US15/217,117 US201615217117A US2016338368A1 US 20160338368 A1 US20160338368 A1 US 20160338368A1 US 201615217117 A US201615217117 A US 201615217117A US 2016338368 A1 US2016338368 A1 US 2016338368A1
- Authority
- US
- United States
- Prior art keywords
- catechins
- green tea
- solution
- tea extract
- salt
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 239000003755 preservative agent Substances 0.000 title claims abstract description 69
- 230000002335 preservative effect Effects 0.000 title claims abstract description 69
- 235000012055 fruits and vegetables Nutrition 0.000 title claims abstract description 20
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 164
- 235000020688 green tea extract Nutrition 0.000 claims abstract description 132
- 229940094952 green tea extract Drugs 0.000 claims abstract description 132
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 claims abstract description 109
- 235000005487 catechin Nutrition 0.000 claims abstract description 109
- 150000001765 catechin Chemical class 0.000 claims abstract description 101
- 235000010323 ascorbic acid Nutrition 0.000 claims abstract description 75
- 239000011668 ascorbic acid Substances 0.000 claims abstract description 75
- 229960005070 ascorbic acid Drugs 0.000 claims abstract description 71
- 150000007524 organic acids Chemical class 0.000 claims abstract description 34
- 150000003839 salts Chemical class 0.000 claims abstract description 31
- 235000010350 erythorbic acid Nutrition 0.000 claims abstract description 17
- CIWBSHSKHKDKBQ-DUZGATOHSA-N D-araboascorbic acid Natural products OC[C@@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-DUZGATOHSA-N 0.000 claims abstract description 15
- 239000004318 erythorbic acid Substances 0.000 claims abstract description 15
- 229940026239 isoascorbic acid Drugs 0.000 claims abstract description 15
- 235000002639 sodium chloride Nutrition 0.000 claims abstract description 7
- 239000000243 solution Substances 0.000 claims description 101
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 42
- 238000000034 method Methods 0.000 claims description 30
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 14
- 235000013399 edible fruits Nutrition 0.000 claims description 14
- 235000013311 vegetables Nutrition 0.000 claims description 14
- 239000007864 aqueous solution Substances 0.000 claims description 13
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 12
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- 239000003945 anionic surfactant Substances 0.000 claims description 9
- WMBWREPUVVBILR-UHFFFAOYSA-N GCG Natural products C=1C(O)=C(O)C(O)=CC=1C1OC2=CC(O)=CC(O)=C2CC1OC(=O)C1=CC(O)=C(O)C(O)=C1 WMBWREPUVVBILR-UHFFFAOYSA-N 0.000 claims description 8
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 8
- RYYVLZVUVIJVGH-UHFFFAOYSA-N caffeine Chemical compound CN1C(=O)N(C)C(=O)C2=C1N=CN2C RYYVLZVUVIJVGH-UHFFFAOYSA-N 0.000 claims description 8
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Chemical compound [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims description 8
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical group [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims description 7
- 239000012298 atmosphere Substances 0.000 claims description 7
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 7
- 235000019333 sodium laurylsulphate Nutrition 0.000 claims description 7
- WMBWREPUVVBILR-WIYYLYMNSA-N (-)-Epigallocatechin-3-o-gallate Chemical compound O([C@@H]1CC2=C(O)C=C(C=C2O[C@@H]1C=1C=C(O)C(O)=C(O)C=1)O)C(=O)C1=CC(O)=C(O)C(O)=C1 WMBWREPUVVBILR-WIYYLYMNSA-N 0.000 claims description 6
- 229930014124 (-)-epigallocatechin gallate Natural products 0.000 claims description 6
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 claims description 5
- 238000004806 packaging method and process Methods 0.000 claims description 5
- LPHGQDQBBGAPDZ-UHFFFAOYSA-N Isocaffeine Natural products CN1C(=O)N(C)C(=O)C2=C1N(C)C=N2 LPHGQDQBBGAPDZ-UHFFFAOYSA-N 0.000 claims description 4
- 229960001948 caffeine Drugs 0.000 claims description 4
- VJEONQKOZGKCAK-UHFFFAOYSA-N caffeine Natural products CN1C(=O)N(C)C(=O)C2=C1C=CN2C VJEONQKOZGKCAK-UHFFFAOYSA-N 0.000 claims description 4
- 235000010376 calcium ascorbate Nutrition 0.000 claims description 4
- 229940047036 calcium ascorbate Drugs 0.000 claims description 4
- 239000011692 calcium ascorbate Substances 0.000 claims description 4
- 235000010216 calcium carbonate Nutrition 0.000 claims description 4
- BLORRZQTHNGFTI-ZZMNMWMASA-L calcium-L-ascorbate Chemical compound [Ca+2].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-] BLORRZQTHNGFTI-ZZMNMWMASA-L 0.000 claims description 4
- 239000011736 potassium bicarbonate Substances 0.000 claims description 4
- 229910000028 potassium bicarbonate Inorganic materials 0.000 claims description 4
- 235000015497 potassium bicarbonate Nutrition 0.000 claims description 4
- 229910000027 potassium carbonate Inorganic materials 0.000 claims description 4
- 235000011181 potassium carbonates Nutrition 0.000 claims description 4
- TYJJADVDDVDEDZ-UHFFFAOYSA-M potassium hydrogencarbonate Chemical compound [K+].OC([O-])=O TYJJADVDDVDEDZ-UHFFFAOYSA-M 0.000 claims description 4
- 235000011118 potassium hydroxide Nutrition 0.000 claims description 4
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 4
- 235000017550 sodium carbonate Nutrition 0.000 claims description 4
- 235000011121 sodium hydroxide Nutrition 0.000 claims description 4
- 239000004599 antimicrobial Substances 0.000 claims 2
- 238000010186 staining Methods 0.000 abstract description 11
- 239000000796 flavoring agent Substances 0.000 abstract description 10
- 235000019634 flavors Nutrition 0.000 abstract description 9
- 241000220225 Malus Species 0.000 description 39
- 235000021452 apple slice Nutrition 0.000 description 32
- 238000011282 treatment Methods 0.000 description 26
- 241000366676 Justicia pectoralis Species 0.000 description 25
- 244000061456 Solanum tuberosum Species 0.000 description 23
- 235000002595 Solanum tuberosum Nutrition 0.000 description 23
- 235000011430 Malus pumila Nutrition 0.000 description 20
- 235000015103 Malus silvestris Nutrition 0.000 description 20
- 238000003860 storage Methods 0.000 description 20
- 235000021016 apples Nutrition 0.000 description 19
- 235000013305 food Nutrition 0.000 description 15
- 230000003078 antioxidant effect Effects 0.000 description 13
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 12
- 238000002845 discoloration Methods 0.000 description 12
- 239000003963 antioxidant agent Substances 0.000 description 11
- 235000006708 antioxidants Nutrition 0.000 description 11
- 230000000694 effects Effects 0.000 description 10
- 229930003935 flavonoid Natural products 0.000 description 10
- 150000002215 flavonoids Chemical class 0.000 description 10
- 235000017173 flavonoids Nutrition 0.000 description 10
- 239000000843 powder Substances 0.000 description 10
- 239000000203 mixture Substances 0.000 description 9
- 235000012015 potatoes Nutrition 0.000 description 9
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 description 8
- 229950001002 cianidanol Drugs 0.000 description 8
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 7
- 230000000845 anti-microbial effect Effects 0.000 description 7
- 238000004321 preservation Methods 0.000 description 7
- 244000269722 Thea sinensis Species 0.000 description 6
- 150000001875 compounds Chemical class 0.000 description 6
- 238000009472 formulation Methods 0.000 description 6
- 238000012545 processing Methods 0.000 description 6
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 6
- 235000017557 sodium bicarbonate Nutrition 0.000 description 6
- 102000030523 Catechol oxidase Human genes 0.000 description 5
- 108010031396 Catechol oxidase Proteins 0.000 description 5
- LSNNMFCWUKXFEE-UHFFFAOYSA-L sulfite Chemical class [O-]S([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-L 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 4
- 229940072107 ascorbate Drugs 0.000 description 4
- 230000002255 enzymatic effect Effects 0.000 description 4
- 239000004033 plastic Substances 0.000 description 4
- 239000003761 preservation solution Substances 0.000 description 4
- 230000009467 reduction Effects 0.000 description 4
- 239000000344 soap Substances 0.000 description 4
- 230000009044 synergistic interaction Effects 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 240000008415 Lactuca sativa Species 0.000 description 3
- 235000003228 Lactuca sativa Nutrition 0.000 description 3
- 241000220324 Pyrus Species 0.000 description 3
- 239000006172 buffering agent Substances 0.000 description 3
- 230000015556 catabolic process Effects 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 238000006731 degradation reaction Methods 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 238000007598 dipping method Methods 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 235000009569 green tea Nutrition 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 230000003647 oxidation Effects 0.000 description 3
- 238000007254 oxidation reaction Methods 0.000 description 3
- 150000008442 polyphenolic compounds Chemical class 0.000 description 3
- 235000013616 tea Nutrition 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- -1 U.S. Pat. No. 2 Chemical class 0.000 description 2
- 150000008051 alkyl sulfates Chemical class 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229940026231 erythorbate Drugs 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 229910021645 metal ion Inorganic materials 0.000 description 2
- 235000021017 pears Nutrition 0.000 description 2
- 235000013824 polyphenols Nutrition 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 239000001100 (2S)-5,7-dihydroxy-2-(3-hydroxy-4-methoxyphenyl)chroman-4-one Substances 0.000 description 1
- WVXRAFOPTSTNLL-NKWVEPMBSA-N 2',3'-dideoxyadenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1CC[C@@H](CO)O1 WVXRAFOPTSTNLL-NKWVEPMBSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 description 1
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- 241000222122 Candida albicans Species 0.000 description 1
- 235000014036 Castanea Nutrition 0.000 description 1
- 241001070941 Castanea Species 0.000 description 1
- DQFBYFPFKXHELB-UHFFFAOYSA-N Chalcone Natural products C=1C=CC=CC=1C(=O)C=CC1=CC=CC=C1 DQFBYFPFKXHELB-UHFFFAOYSA-N 0.000 description 1
- 241000207199 Citrus Species 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- CITFYDYEWQIEPX-UHFFFAOYSA-N Flavanol Natural products O1C2=CC(OCC=C(C)C)=CC(O)=C2C(=O)C(O)C1C1=CC=C(O)C=C1 CITFYDYEWQIEPX-UHFFFAOYSA-N 0.000 description 1
- QUQPHWDTPGMPEX-UHFFFAOYSA-N Hesperidine Natural products C1=C(O)C(OC)=CC=C1C1OC2=CC(OC3C(C(O)C(O)C(COC4C(C(O)C(O)C(C)O4)O)O3)O)=CC(O)=C2C(=O)C1 QUQPHWDTPGMPEX-UHFFFAOYSA-N 0.000 description 1
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 240000003394 Malpighia glabra Species 0.000 description 1
- 235000014837 Malpighia glabra Nutrition 0.000 description 1
- 241000198694 Passiflora pallida Species 0.000 description 1
- 235000008673 Persea americana Nutrition 0.000 description 1
- 244000025272 Persea americana Species 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 235000014443 Pyrus communis Nutrition 0.000 description 1
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 240000001717 Vaccinium macrocarpon Species 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000010306 acid treatment Methods 0.000 description 1
- 235000019647 acidic taste Nutrition 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229930014669 anthocyanidin Natural products 0.000 description 1
- 235000008758 anthocyanidins Nutrition 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- QUQPHWDTPGMPEX-UTWYECKDSA-N aurantiamarin Natural products COc1ccc(cc1O)[C@H]1CC(=O)c2c(O)cc(O[C@@H]3O[C@H](CO[C@@H]4O[C@@H](C)[C@H](O)[C@@H](O)[C@H]4O)[C@@H](O)[C@H](O)[C@H]3O)cc2O1 QUQPHWDTPGMPEX-UTWYECKDSA-N 0.000 description 1
- 229910052788 barium Inorganic materials 0.000 description 1
- DSAJWYNOEDNPEQ-UHFFFAOYSA-N barium atom Chemical compound [Ba] DSAJWYNOEDNPEQ-UHFFFAOYSA-N 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 229940095731 candida albicans Drugs 0.000 description 1
- 235000021466 carotenoid Nutrition 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
- 235000005513 chalcones Nutrition 0.000 description 1
- 150000001789 chalcones Chemical class 0.000 description 1
- 235000020971 citrus fruits Nutrition 0.000 description 1
- APSNPMVGBGZYAJ-GLOOOPAXSA-N clematine Natural products COc1cc(ccc1O)[C@@H]2CC(=O)c3c(O)cc(O[C@@H]4O[C@H](CO[C@H]5O[C@@H](C)[C@H](O)[C@@H](O)[C@H]5O)[C@@H](O)[C@H](O)[C@H]4O)cc3O2 APSNPMVGBGZYAJ-GLOOOPAXSA-N 0.000 description 1
- 229920002770 condensed tannin Polymers 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 235000021019 cranberries Nutrition 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 150000002206 flavan-3-ols Chemical class 0.000 description 1
- 235000011987 flavanols Nutrition 0.000 description 1
- 229930003949 flavanone Natural products 0.000 description 1
- 150000002208 flavanones Chemical class 0.000 description 1
- 235000011981 flavanones Nutrition 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002212 flavone derivatives Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- HVQAJTFOCKOKIN-UHFFFAOYSA-N flavonol Natural products O1C2=CC=CC=C2C(=O)C(O)=C1C1=CC=CC=C1 HVQAJTFOCKOKIN-UHFFFAOYSA-N 0.000 description 1
- 150000002216 flavonol derivatives Chemical class 0.000 description 1
- 235000011957 flavonols Nutrition 0.000 description 1
- NWKFECICNXDNOQ-UHFFFAOYSA-N flavylium Chemical compound C1=CC=CC=C1C1=CC=C(C=CC=C2)C2=[O+]1 NWKFECICNXDNOQ-UHFFFAOYSA-N 0.000 description 1
- 235000013355 food flavoring agent Nutrition 0.000 description 1
- 238000009920 food preservation Methods 0.000 description 1
- 235000021022 fresh fruits Nutrition 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 229940093915 gynecological organic acid Drugs 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- QUQPHWDTPGMPEX-QJBIFVCTSA-N hesperidin Chemical compound C1=C(O)C(OC)=CC=C1[C@H]1OC2=CC(O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@@H](CO[C@H]4[C@@H]([C@H](O)[C@@H](O)[C@H](C)O4)O)O3)O)=CC(O)=C2C(=O)C1 QUQPHWDTPGMPEX-QJBIFVCTSA-N 0.000 description 1
- 229940025878 hesperidin Drugs 0.000 description 1
- VUYDGVRIQRPHFX-UHFFFAOYSA-N hesperidin Natural products COc1cc(ccc1O)C2CC(=O)c3c(O)cc(OC4OC(COC5OC(O)C(O)C(O)C5O)C(O)C(O)C4O)cc3O2 VUYDGVRIQRPHFX-UHFFFAOYSA-N 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 229930013032 isoflavonoid Natural products 0.000 description 1
- 150000003817 isoflavonoid derivatives Chemical class 0.000 description 1
- 235000012891 isoflavonoids Nutrition 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229910052744 lithium Inorganic materials 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000009448 modified atmosphere packaging Methods 0.000 description 1
- ARGKVCXINMKCAZ-UHFFFAOYSA-N neohesperidine Natural products C1=C(O)C(OC)=CC=C1C1OC2=CC(OC3C(C(O)C(O)C(CO)O3)OC3C(C(O)C(O)C(C)O3)O)=CC(O)=C2C(=O)C1 ARGKVCXINMKCAZ-UHFFFAOYSA-N 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000003002 pH adjusting agent Substances 0.000 description 1
- 235000010204 pine bark Nutrition 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 235000005875 quercetin Nutrition 0.000 description 1
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 235000005493 rutin Nutrition 0.000 description 1
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 1
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 1
- 229960004555 rutoside Drugs 0.000 description 1
- 238000011012 sanitization Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 229910052712 strontium Inorganic materials 0.000 description 1
- CIOAGBVUUVVLOB-UHFFFAOYSA-N strontium atom Chemical compound [Sr] CIOAGBVUUVVLOB-UHFFFAOYSA-N 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- 238000010610 time kill assay Methods 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Definitions
- This invention relates to the preservation of fruits and vegetables, including minimally processed produce, using a preservative solution containing green tea extract catechins and an organic acid.
- the preservative solution prevents residual self-staining commonly associated with green tea extract catechins.
- the discoloration is typically due to enzymes within the fruits or vegetables that metabolize colorless endogenous chemical compounds into colored chemical compounds that lead to the discoloration, often a browning.
- a major enzyme responsible for browning in many fruits and vegetables is polyphenoloxidase.
- Ascorbic acid is an antioxidant and prevents the enzymatic oxidation, and coloration, of endogenous chemical compounds in the fruit or vegetable; however, the effect is short lived or requires very high concentrations of antioxidant (Gil et al., “Response of Fuji Apple Slices to Ascorbic Acid Treatments and Low-Oxygen Atmospheres,” HortScience, volume 33, number 2, 1998, pp. 05-309).
- U.S. Pat. Nos. 5,925,395 and 5,939,117 disclose treating fresh cut fruit and vegetables with a combination of ascorbate (or erythorbate) ions and calcium ions. Preservation lasts several weeks; however, an excessive amount of ascorbate ion in some embodiments (up to 22.5% in the preservation solution) is needed for preservation.
- U.S. Pat. Nos. 7,851,002, 8,101,221, and 8,609,169 take a similar approach of combining ascorbic acid/ascorbate (or erythorbic acid/erythorbate) with various metal ions such as magnesium, zinc, strontium, lithium, barium, aluminum, copper, iron, ammonium, manganese, and potassium. Many of these metal ions are not preferred for a food preservation product and a large concentration of preservative is required.
- U.S. Pat. No. 5,922,382 discloses a method for preserving fresh cut fruit using ascorbic acid.
- the method is labor intensive and involves the use of sanitizing and flavoring agents.
- the concentration of ascorbic or erythorbic acid in the preservation solution is high (up to 15% weight/volume).
- U.S. Pat. No. 6,749,875 (and patent application US 2002/0054950) and international patent WO 01/64041 use a combination of flavonoid and food grade antioxidant in a ratio by weight of 1:50 to 1:150.
- the flavonoids listed in the patent claims include acerola, quercitin, hesperidin, rutin, and flavonoid rich extracts from pine bark, grape seeds, citrus seeds, cranberries, Juniper berries and rosehips.
- flavonoids which is technically a very broad and general classification for a wide array of plant-derived polyphenolic compounds, as flavone, flavonols, flavanones, chalcones, anthocyanidins, proanthocyanidins, and isoflavonoids.
- the US patent states that the flavonoid and antioxidant can be diluted in water, tea infusion, or fruit juice for preparation of the fruit or vegetable preservation solution.
- the US patent discloses two formulations that include a flavonoid source, an antioxidant, and a water+tea dilution solution.
- one formulation for treating chestnuts included 90 mg enzogenol (flavonoid), 5 g calcium ascorbate (antioxidant), and 200 ml water+5 mg green tea (dilution solution).
- green tea was included, it was at a very low concentration (0.0025 wt %) and was not taught for the purposes of supplying flavonoids since the flavonoids in green tea are primarily catechins, which are not listed as applicable flavonoids in the patent.
- the patent instead states that tea is used as a dilution solution.
- the patent examples required modified atmosphere packaging for packed fruit, whereas, it is desirable to develop an antibrowning preservative that does not have special atmosphere handling or packaging requirements.
- catechins also known as flavanols
- green tea extract catechins were not included at significant levels in the preservative formulations of U.S. Pat. No. 6,749,875 and international patent WO 01/64041, since catechins have a strong brown color and can result in significant discoloration of produce, particularly light colored flesh, such as apples, pears, and potatoes, when used as a treatment (e.g., dipping) solution.
- a treatment e.g., dipping
- green tea extract catechins are antioxidants and can reduce the enzymatic oxidation of colorless endogenous chemical compounds within the fruit or vegetable into colored, typically brown, chemical compounds (Kim et al., “New Insights Into the Mechanisms of Polyphenols Beyond Antioxidant Properties; Lessons from the Green Tea Polyphenol, Epigallocatechin 3-Gallate,” Redox Biology, volume 2, 2014, pp. 187-195).
- green tea extract catechins have been shown to inhibit polyphenoloxidase, which is a key enzyme involved in the production of colored chemical compounds within the fruit or vegetable (Soysal, “Effects of Green Tea Extract on Golden Delicious Apple Polyphenoloxidase and its Browning,” J.
- the stability of green tea extract catechins is affected by various factors, with pH being a critical component (Chen et al. “Stabilizing Effect of Ascorbic Acid on Green Tea Catechins,” J. Agri. Food Chem., volume 46, number 7, 1998, pp. 2512-2516; Zhu et al. “Stability of Green Tea Catechins,” J. of Agricultural and Food Chemistry, volume 45, issue 12, 1997, pp. 4624-4628; Li et al. “Kinetic Study of Catechin Stability: Effects of pH, Concentration, and Temperature,” J. of Agricultural and Food Chemistry, volume 60, issue 51, 2012, pp. 12531-12539).
- the invention is directed towards a method of preserving minimally processed fruits and vegetables and extending their useful shelf-life comprising: (a) providing an aqueous preservative solution comprising (i) at least 0.008 wt percent catechins and (ii) an organic acid or salt thereof selected from any one or any combination of ascorbic acid, erythorbic acid, and salts thereof; wherein the weight ratio of catechins to organic acid or salt thereof is from 1:10 to 1:300; and (b) applying the preservative solution to a minimally processed fruit or vegetable.
- the invention is directed towards an aqueous solution for preserving minimally processed fruits and vegetables and extending their useful shelf-life consisting essentially of (i) water; (ii) green tea extract; (iii) an organic acid or salt thereof selected from any one or any combination of ascorbic acid, erythorbic acid, and salts thereof; optionally (iv) a basic neutralizer approved for food use selected from the group consisting of: sodium hydroxide, potassium hydroxide, sodium carbonate, sodium bicarbonate, potassium carbonate, potassium bicarbonate, and calcium carbonate; and, optionally (v) a food-safe anionic surfactant; wherein the green tea extract comprises catechins, the catechins are present at a concentration of at least 0.008 wt % of the solution, and the weight ratio of catechins to organic acid or salt thereof in the solution is from 1:10 to 1:300.
- the present invention enables extending the shelf life and appearance of minimally processed fruits and vegetables, as well as normal handling, packaging, and storage of the preserved fruits and vegetables under normal atmosphere and pressure conditions.
- this invention is partly predicated by the ability of catechins, and in particular green tea extract catechins, to act as antioxidants and inhibit polyphenoloxidase.
- organic acids particularly acids with antioxidant properties such as ascorbic acid and its isomer erythorbic acid, interact with the catechins in green tea extract in a synergistic manner to prevent the browning of processed produce, including minimally processed produce, such as apple or potato slices.
- Application of either the organic acid or green tea extract catechins alone results in less than satisfactory antibrowning properties and in the case of green tea extract catechins, results in significant self-staining.
- the organic acid surprisingly prevents the self-staining discoloration normally associated with produce treated with green tea extract catechins when used in amounts effective to provide antibrowning of the treated produce.
- This latter finding is critical for acceptance of a green tea extract catechin produce preservative solution, particularly for use on fruits and vegetables with light colored or white flesh, such as apple, pear, or potato slices.
- the present invention advantageously enables use of a relatively much lower concentration of preservative to be employed in order to provide the desired antibrowning effect over extended time periods.
- minimally processed fruits and vegetables may be treated with a preservative solution containing green tea extract catechins and an organic acid or salt thereof with antioxidant properties selected from any one or any combination of ascorbic acid, erythorbic acid, and salts thereof.
- the fruits or vegetables can be treated with a variety of methods such as dipping, spraying, or coating with the preservative solution. After treatment, the fruits and vegetables can then be packed and stored using conventional procedures under normal atmosphere and pressure conditions. Treatment with the preservative solution thus advantageously extends the shelf-life of the produce without the use of special handling or packaging requirements.
- the present invention pertains to the use of a preservative solution that retains the natural color, texture, and flavor of minimally processed produce such as peeled and/or cut fruits and vegetables.
- the invention applies to all types of minimally processed fruits and vegetables and is particularly useful for processed produce that degrades rapidly shortly after processing such as apples, pears, avocados, potatoes, and mushrooms.
- Preserved fruits and vegetables are prevented from browning and retain their natural color, texture, and flavor under normal storage conditions, such as refrigeration under normal atmosphere and pressure.
- the preservative solution is comprised of catechins and an organic acid.
- the catechins preferably comprise at least 25 wt % epigallocatechin-3-gallate (EGCG), and are preferably provided by green tea extract having a catechin content of at least 50 wt %, more preferably at least 75 wt %, and more particularly an epigallocatechin-3-gallate (EGCG) content of at least 20 wt %, more preferably at least 40 wt %.
- the green tea extract also preferably contains less than 25 wt % caffeine, more preferably less than 10 wt % caffeine.
- the organic acid content can be provided by one or more of ascorbic acid, erythorbic acid, or a salt thereof.
- the final, diluted preservative solution applied to produce in accordance with the invention contains at least 0.008 wt % catechins, and preferably contains 0.008% to 10% green tea extract catechins, more preferably at least 0.02%, at least 0.05%, or at least 0.08%, and more preferably at most 2%, at most 1%, or at most 0.5% green tea extract catechins.
- the final, diluted preservative solution preferably contains 0.2% to 20% organic acid or salt thereof, more preferably at least 0.5%, at least 1%, or at least 2%, and more preferably at most 10%, at most 7% or at most 5% organic acid or salt thereof.
- the weight ratio of catechins to organic acid or salt thereof is from 1:10 to 1:300, more preferably from about 1:100 to 1:300, as use of more than 300 parts organic acid to 1 part catechin is not needed to provide the observed preservative effect, and as use of less than 10 parts organic acid to 1 part catechin may not provide as great as desired effect.
- all percentages and component ratios further indicated herein are also weight (wt) percentages and weight (wt) ratios.
- the pH of the preservative solution can be increased with a buffering agent such that the pH of the final, diluted preservative solution is still acidic (i.e., ⁇ 7.0). Adjusting the pH decreases the acidic taste of the preservative solution. This is important for preservative solutions with very high ascorbic acid concentrations; however, since the preservative solutions described in accordance with the invention enable relatively low ascorbic acid concentrations due to the synergistic interaction with the green tea extract catechins, neutralization may not be required.
- the buffering agent can be any basic neutralizing agent acceptable for use on food such as sodium hydroxide, potassium hydroxide, calcium carbonate, sodium carbonate, sodium bicarbonate, potassium carbonate, or potassium bicarbonate.
- a combination of the organic acid and any salt form of the organic acid can be combined in a ratio that results in the target pH.
- a food-safe anionic surfactant can be added to the formulation preferably at 0.001% to 0.5%, more preferably at least 0.02%, at least 0.04%, or at least 0.08%, and more preferably at most 0.2%, at most 0.15%, or at most 0.1%.
- Useful anionic surfactants include, e.g., sulfated fatty acids such as C6-C18 alkyl sulfates, more particularly C8-C14 alkyl sulfates, and in particular sodium lauryl sulfate.
- the preservative solution can be prepared by combining ingredients at final ready-to-use concentrations, or by diluting previously made concentrated solutions or powder mixtures with added water.
- powder and concentrated solutions the amount of green tea extract catechins, organic acid, and buffering agent in the powder or concentrated solution are increased in proportion to the final use concentrations.
- the use of concentrated solutions allow end users to prepare larger quantities of end-use solutions from small amounts of concentrated solution, which facilitates shipping and handling by minimizing product volume.
- the concentrated solution compositions may be designed to be diluting with at least one, and more preferably at least 9 additional parts of water to one part of the concentrate composition to form a diluted end use solution.
- the concentration of green tea extract catechins in the 10 ⁇ concentrated solution would be 1% and the concentration of ascorbic acid would be 30%.
- the preservative powder or concentrate is diluted in normal processing water to the desired use concentration.
- the processed produce is then treated with the preservative solution by dipping, spraying, coating, or any other means that results in adequate exposure of the cut or peeled surface of the produce to the preservative solution.
- the preserved produce may then be packaged and stored using conventional procedures and temperatures.
- the preserved produce is stored between ⁇ 6° C. to 20° C., preferably, ⁇ 3 to 10° C., and most preferably 0 to 6° C., under normal atmosphere content and pressure.
- ⁇ 6° C. to 20° C. preferably, ⁇ 3 to 10° C., and most preferably 0 to 6° C.
- the green tea extract (GTE) employed in all examples comprised 83.2% total catechins, and more particularly 50.1% EGCG, and 1.8% caffeine.
- GTE green tea extract
- water can be eliminated from each example to provide a concentrated powder.
- the ready-to-use liquid antibrowning solution is prepared by adding the indicated amount of water to the indicated total weight of powder.
- a ready-to-use solution is prepared by combining the following in water:
- a ready-to-use solution is prepared by combining the following in water:
- a ready-to-use solution is prepared by combining the following in water:
- a 5-fold concentrated solution is prepared by combining the following in water:
- a ready-to-use solution is prepared by combining the following in water:
- a 10-fold concentrated solution is prepared by combining the following in water:
- a ready-to-use solution is prepared by combining the following in water:
- a ready-to-use solution is prepared by combining the following in water:
- a concentrated powder is prepared by combining the following ingredients. When used to prepare the final antibrowning solution, 31.4 g of powder is added to 1 liter of water:
- a ready-to-use solution is prepared by combining the following in water:
- Preliminary testing was conducted with aqueous solutions of various concentrations of ascorbic acid, green tea extract, and combinations of ascorbic acid and green tea extract as noted in Table 1 below to determine the ability of ascorbic acid to prevent the brown discoloration associated with green tea extract catechins, and determine the optimal concentrations of combinations of ascorbic acid and green tea extract catechins for preventing the browning of red delicious apple slices.
- Table 1 concentrations of ascorbic acid, green tea extract, and combinations of ascorbic acid and green tea extract catechins
- Green tea extract at 0.003% did not discolor the flesh of the apple slices; however, it also did not provide a synergistic interaction with ascorbic acid in enhancing the anti-browning effect (Table 1).
- green tea extract at 0.01% i.e., C approximately 0.008% catechins
- stained the flesh of apple slices brown however, the addition of 3% ascorbic acid prevented this brown staining (Table 1).
- green tea extract at ⁇ 0.01% had a strong synergistic interaction with 3% ascorbic acid and maintained the appearance of the apple slices similar to the white flesh of fresh cut apple slices over 2 days storage at 4° C. (Table 1).
- the preservation effect was tested for longer durations as outlined below.
- Color was assessed either by a qualitative rating by eye or quantitatively using a digital color scanner after the specified period of storage.
- the cut surface of each apple or potato slice was digitized as a color bitmap image at a resolution of 200 dots per inch using 16-bit red, green, blue (RGB) color.
- RGB red, green, blue
- a series of white, several gradations of grey, and black color swatches were scanned with each scan.
- the average individual RGB signals were measured for each apple or potato slice using the publicly available program NIH Image J 1.47v (available at imagej.nih.gov/ij). Whiteness was assessed as the average of the red+green+blue values.
- the flavor, texture, and moistness of the preserved apple slices were qualitatively assessed by tasting the slices after 14 days of storage and comparing them to fresh cut apple slices.
- color and appearance were also rated qualitatively and compared to fresh cut apples or potatoes on each assessment day.
- Firmness of the apple slices was assessed after 14 days of storage using a manual penetrometer and compared to fresh cut apple slices.
- Apples slices were prepared by using a manual apple corer/slicer that prepared 10 slices per apple. Apple slices were randomly mixed from multiple apples and then placed in each preservative solution, 1 or 3% ascorbic acid, 0.1% green tea extract (83% catechins), or left untreated for approximately 5 minutes. The slices were then placed in plastic containers and stored at approximately 4° C. After the indicated time, one cut surface of each slice was digitized. In addition, the color, general appearance, and smell were assessed qualitatively at the indicated times and compared to fresh cut apple slices. After 14 days of storage, apple slices were assessed for firmness quantitatively using a manual penetrometer. After 14 days of storage, flavor, moisture, and texture were qualitatively assessed only for slices that had normal appearance by tasting each slice.
- Apples slices were prepared by using a manual apple corer/slicer that prepared 10 slices per apple. Apple slices were randomly mixed from multiple apples and then placed in each preservative solution or 3% ascorbic acid for approximately 5 minutes. The slices were then placed in plastic containers and stored at approximately 4° C. After the indicated time, the slices were qualitatively assessed for appearance.
- Red delicious apples were washed thoroughly with soap and water and rinsed thoroughly with water after washing.
- the apples were manually peeled and then slices prepared using a manual apple corer/slicer that prepared 10 slices per apple.
- Apple slices were randomly mixed from multiple apples and then placed in the preservative solution or 3% ascorbic acid for approximately 5 minutes. The slices were then placed in a plastic container and stored at approximately 4° C. After the indicated time, the entire slice (peeled and cut surfaces) was qualitatively assessed for appearance.
- the efficacy of the preservative solution was tested on baking potatoes, which are a difficult vegetable to prevent from browning.
- the following ready-to-use preservative solutions were prepared in water:
- Baking potatoes were washed thoroughly with soap and water and rinsed thoroughly with water after washing. The potatoes were peeled and then rinsed with water.
- the potato slices were prepared by manual slicing with a knife. Slices were randomly mixed from multiple potatoes and then placed in preservative solution, 4% ascorbic acid, or 0.1% green tea extract (83% catechins) for approximately 5 minutes. The slices were then placed in plastic containers and stored at approximately 4° C. After the indicated time, one cut surface of each slice was digitized. In addition, the color and general appearance were assessed qualitatively at the indicated times and compared to fresh cut potato slices.
- An antibrowning solution with improved antimicrobial properties can be prepared by adding a food-safe anionic surfactant such as sodium lauryl sulfate.
- a food-safe anionic surfactant such as sodium lauryl sulfate.
- the antimicrobial properties of the three solutions from Examples 8, 9, and 10 was assessed against a yeast that is problematic in food processing operations, Candida albicans .
- the effectiveness was assessed in a suspension time kill assay. In brief, the microbial suspension (1 ⁇ 10 7 CFU/ml) was added to 9.9 times the volume of 1 ⁇ end-use antibrowning/antimicrobial solution. After 15 minutes, an aliquot of the test solution was removed and neutralized with culture broth. The neutralized solution containing yeast was then plated on solid agar medium and allowed to incubate at 36 ⁇ 1° C. for 48 hours to identify viable colonies.
- Table 10 summarizes the results of duplicate replicates including the log-fold reduction in yeast for the two replicates combined for each solution. All example formulations containing the anionic surfactant (Examples 8, 9, and 10) provided dramatic reductions in yeast counts with no surviving yeast detected after treatment with the solutions. The solution from Example 2 (no anionic surfactant) was included to demonstrate the importance of adding the anionic surfactant for achieving improved antimicrobial properties, as the solution in Example 2 provided only a minimal reduction of yeast counts.
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Storage Of Fruits Or Vegetables (AREA)
- Preparation Of Fruits And Vegetables (AREA)
Abstract
Minimally processed fruits and vegetables, such as peeled and cut produce, are preserved by a solution containing a combination of green tea extract catechins and an organic acid or salt thereof selected from any one or any combination of ascorbic acid, erythorbic acid, and salts thereof. The preservative solution prevents residual self-staining properties commonly associated with green tea extract catechins. Processed fruits and vegetables are dipped in, sprayed with, or coated with the preservative solution. The produce preservative solution maintains the color, texture, flavor, appearance, and crispness of the treated produce.
Description
- This patent application is a continuation patent application of U.S. patent application Ser. No. 14/621,793, filed Feb. 13, 2015, which is incorporated herein by reference in its entirety for all purposes.
- This invention relates to the preservation of fruits and vegetables, including minimally processed produce, using a preservative solution containing green tea extract catechins and an organic acid. The preservative solution prevents residual self-staining commonly associated with green tea extract catechins.
- After processing, such as peeling or cutting, most fruits and vegetables discolor. The discoloration is typically due to enzymes within the fruits or vegetables that metabolize colorless endogenous chemical compounds into colored chemical compounds that lead to the discoloration, often a browning. A major enzyme responsible for browning in many fruits and vegetables is polyphenoloxidase.
- Various methods have been used to prevent the browning of processed produce. A very effective means of preserving produce is through the use of sulfites (e.g. U.S. Pat. No. 2,894,843 discloses the treatment of fruits and vegetables with various sulfites). Sulfites were widely used for preserving minimally processed fresh fruits and vegetables; however, due to health concerns, the US Food and Drug Administration no longer allows the use of sulfites for the preservation of fresh, including minimally processed, produce.
- Since sulfites are no longer allowed for preserving fresh produce, alternative methods have been developed. A commonly used approach is treating the processed produce with ascorbic acid or an ascorbate sale (e.g. see U.S. Pat. Nos. 3,754,938 and 4,011,348). Ascorbic acid is an antioxidant and prevents the enzymatic oxidation, and coloration, of endogenous chemical compounds in the fruit or vegetable; however, the effect is short lived or requires very high concentrations of antioxidant (Gil et al., “Response of Fuji Apple Slices to Ascorbic Acid Treatments and Low-Oxygen Atmospheres,” HortScience, volume 33, number 2, 1998, pp. 05-309).
- U.S. Pat. Nos. 5,925,395 and 5,939,117 disclose treating fresh cut fruit and vegetables with a combination of ascorbate (or erythorbate) ions and calcium ions. Preservation lasts several weeks; however, an excessive amount of ascorbate ion in some embodiments (up to 22.5% in the preservation solution) is needed for preservation.
- U.S. Pat. Nos. 7,851,002, 8,101,221, and 8,609,169 take a similar approach of combining ascorbic acid/ascorbate (or erythorbic acid/erythorbate) with various metal ions such as magnesium, zinc, strontium, lithium, barium, aluminum, copper, iron, ammonium, manganese, and potassium. Many of these metal ions are not preferred for a food preservation product and a large concentration of preservative is required.
- U.S. Pat. No. 5,922,382 discloses a method for preserving fresh cut fruit using ascorbic acid. However, the method is labor intensive and involves the use of sanitizing and flavoring agents. In addition, the concentration of ascorbic or erythorbic acid in the preservation solution is high (up to 15% weight/volume).
- U.S. Pat. No. 6,749,875 (and patent application US 2002/0054950) and international patent WO 01/64041 use a combination of flavonoid and food grade antioxidant in a ratio by weight of 1:50 to 1:150. The flavonoids listed in the patent claims include acerola, quercitin, hesperidin, rutin, and flavonoid rich extracts from pine bark, grape seeds, citrus seeds, cranberries, Juniper berries and rosehips. Furthermore, the patent defines flavonoids, which is technically a very broad and general classification for a wide array of plant-derived polyphenolic compounds, as flavone, flavonols, flavanones, chalcones, anthocyanidins, proanthocyanidins, and isoflavonoids. The US patent states that the flavonoid and antioxidant can be diluted in water, tea infusion, or fruit juice for preparation of the fruit or vegetable preservation solution. The US patent discloses two formulations that include a flavonoid source, an antioxidant, and a water+tea dilution solution. For example, one formulation for treating chestnuts included 90 mg enzogenol (flavonoid), 5 g calcium ascorbate (antioxidant), and 200 ml water+5 mg green tea (dilution solution). Although green tea was included, it was at a very low concentration (0.0025 wt %) and was not taught for the purposes of supplying flavonoids since the flavonoids in green tea are primarily catechins, which are not listed as applicable flavonoids in the patent. The patent instead states that tea is used as a dilution solution. Furthermore, the patent examples required modified atmosphere packaging for packed fruit, whereas, it is desirable to develop an antibrowning preservative that does not have special atmosphere handling or packaging requirements.
- It is not surprising that catechins (also known as flavanols), particularly green tea extract catechins, were not included at significant levels in the preservative formulations of U.S. Pat. No. 6,749,875 and international patent WO 01/64041, since catechins have a strong brown color and can result in significant discoloration of produce, particularly light colored flesh, such as apples, pears, and potatoes, when used as a treatment (e.g., dipping) solution. Despite the residual self-staining caused by the green tea extract catechins, there are several desirable properties of green tea extract catechins that make them particularly suited for preventing the enzymatic discoloration of produce. First, green tea extract catechins are antioxidants and can reduce the enzymatic oxidation of colorless endogenous chemical compounds within the fruit or vegetable into colored, typically brown, chemical compounds (Kim et al., “New Insights Into the Mechanisms of Polyphenols Beyond Antioxidant Properties; Lessons from the Green Tea Polyphenol, Epigallocatechin 3-Gallate,” Redox Biology, volume 2, 2014, pp. 187-195). Second, green tea extract catechins have been shown to inhibit polyphenoloxidase, which is a key enzyme involved in the production of colored chemical compounds within the fruit or vegetable (Soysal, “Effects of Green Tea Extract on Golden Delicious Apple Polyphenoloxidase and its Browning,” J. Food Biochemistry, volume 33, issue 1, 2009, pp. 134-148). The major problem though is the residual self-staining caused by the normal dark color of the green tea extract catechins and this is particularly problematic as the catechins themselves become oxidized over time since oxidation results in an even darker coloration (Wang et al., “Various Antibrowning Agents and Green Tea Extract During Processing and Storage,” J. Food Processing and Preservation, volume 27, issue 3, 2003, pp. 213-225).
- Martin-Diana et al. (“Green Tea Extract as a Natural Antioxidant to Extend the Shelf-Life of Fresh-Cut Lettuce,” Innovative Food Science and Emerging Technologies, volume 9, 2008, pp. 593-603) demonstrated that green tea extract treatment of fresh-cut lettuce helped to prevent the loss of the antioxidants ascorbic acid and carotenoids. However, green tea extract increased the browning of the lettuce in a concentration-dependent manner. Wessels et al. (“Effect of Selected Plant Extracts on the Inhibition of Enzymatic Browning in Fresh-Cut Apple,” Journal of Applied Botany and Food Quality, volume 87, 2014, pp. 16-23) demonstrated that green tea extract could partially retard the browning of apple slices; however, the green tea extract demonstrated significant passive staining (i.e., residual self-staining). In line with many published reports, Wessels et al. (2014) also demonstrated that green extract had a high level of antioxidant activity. Lavelli et al (“Stability and Anti-Glycation Properties of Intermediate Moisture Apple Products Fortified with Green Tea,” Food Chemistry, volume 127, 2011, pp. 589-595) added green tea extract to intermediate moisture apple products and noted that the green tea extract darkened the apple color. Soysal (2009), cited above, demonstrated that green extract inhibited polyphenol oxidase and retarded browning; however, apple browning was only investigated over a 145 minute period and the apples had significant residual self-staining as indicated by a higher browning index immediately after treatment with the green tea extract when compared to the untreated control.
- The stability of green tea extract catechins is affected by various factors, with pH being a critical component (Chen et al. “Stabilizing Effect of Ascorbic Acid on Green Tea Catechins,” J. Agri. Food Chem., volume 46, number 7, 1998, pp. 2512-2516; Zhu et al. “Stability of Green Tea Catechins,” J. of Agricultural and Food Chemistry, volume 45, issue 12, 1997, pp. 4624-4628; Li et al. “Kinetic Study of Catechin Stability: Effects of pH, Concentration, and Temperature,” J. of Agricultural and Food Chemistry, volume 60, issue 51, 2012, pp. 12531-12539). Conflicting reports have demonstrated the role of other additives on the stability of green tea extract catechins themselves. For example, Ortiz et al. (“Interaction of Environmental Moisture with Powdered Green Tea Formulations: Effect on Catechin Chemical Stability,” J. of Agricultural and Food Chemistry, volume 56, issue 11, 2008, pp. 4068-4077) demonstrated that ascorbic acid increased green tea extract catechin degradation; whereas, Chen et al. (1998) demonstrated that ascorbic acid decreased green tea extract catechin degradation. Wang et al. (2003) demonstrated that citric acid decreased the degradation of green tea extract catechins and helped to retard the additional browning of the green tea extract due to the degraded catechins.
- It is an object of this invention to provide an improvement in preserving minimally processed produce using a naturally-derived preservative solution, particularly for preserving light or white colored produce flesh. Furthermore, special handling or packaging should not be required for the preserved produce.
- In accordance with one embodiment, the invention is directed towards a method of preserving minimally processed fruits and vegetables and extending their useful shelf-life comprising: (a) providing an aqueous preservative solution comprising (i) at least 0.008 wt percent catechins and (ii) an organic acid or salt thereof selected from any one or any combination of ascorbic acid, erythorbic acid, and salts thereof; wherein the weight ratio of catechins to organic acid or salt thereof is from 1:10 to 1:300; and (b) applying the preservative solution to a minimally processed fruit or vegetable. In a further embodiment, the invention is directed towards an aqueous solution for preserving minimally processed fruits and vegetables and extending their useful shelf-life consisting essentially of (i) water; (ii) green tea extract; (iii) an organic acid or salt thereof selected from any one or any combination of ascorbic acid, erythorbic acid, and salts thereof; optionally (iv) a basic neutralizer approved for food use selected from the group consisting of: sodium hydroxide, potassium hydroxide, sodium carbonate, sodium bicarbonate, potassium carbonate, potassium bicarbonate, and calcium carbonate; and, optionally (v) a food-safe anionic surfactant; wherein the green tea extract comprises catechins, the catechins are present at a concentration of at least 0.008 wt % of the solution, and the weight ratio of catechins to organic acid or salt thereof in the solution is from 1:10 to 1:300.
- The present invention enables extending the shelf life and appearance of minimally processed fruits and vegetables, as well as normal handling, packaging, and storage of the preserved fruits and vegetables under normal atmosphere and pressure conditions.
- As described in the background information, this invention is partly predicated by the ability of catechins, and in particular green tea extract catechins, to act as antioxidants and inhibit polyphenoloxidase. In accordance with the invention, it has been discovered that organic acids, particularly acids with antioxidant properties such as ascorbic acid and its isomer erythorbic acid, interact with the catechins in green tea extract in a synergistic manner to prevent the browning of processed produce, including minimally processed produce, such as apple or potato slices. Application of either the organic acid or green tea extract catechins alone results in less than satisfactory antibrowning properties and in the case of green tea extract catechins, results in significant self-staining. It has been further discovered, however, that the organic acid surprisingly prevents the self-staining discoloration normally associated with produce treated with green tea extract catechins when used in amounts effective to provide antibrowning of the treated produce. This latter finding is critical for acceptance of a green tea extract catechin produce preservative solution, particularly for use on fruits and vegetables with light colored or white flesh, such as apple, pear, or potato slices. Further, due to the synergistic interaction of the ascorbic acid and green tea extract, it has been demonstrated that the present invention advantageously enables use of a relatively much lower concentration of preservative to be employed in order to provide the desired antibrowning effect over extended time periods.
- In various embodiments, minimally processed fruits and vegetables (e.g., peeled, cored, or sliced) may be treated with a preservative solution containing green tea extract catechins and an organic acid or salt thereof with antioxidant properties selected from any one or any combination of ascorbic acid, erythorbic acid, and salts thereof. The fruits or vegetables can be treated with a variety of methods such as dipping, spraying, or coating with the preservative solution. After treatment, the fruits and vegetables can then be packed and stored using conventional procedures under normal atmosphere and pressure conditions. Treatment with the preservative solution thus advantageously extends the shelf-life of the produce without the use of special handling or packaging requirements.
- The present invention pertains to the use of a preservative solution that retains the natural color, texture, and flavor of minimally processed produce such as peeled and/or cut fruits and vegetables. The invention applies to all types of minimally processed fruits and vegetables and is particularly useful for processed produce that degrades rapidly shortly after processing such as apples, pears, avocados, potatoes, and mushrooms. Preserved fruits and vegetables are prevented from browning and retain their natural color, texture, and flavor under normal storage conditions, such as refrigeration under normal atmosphere and pressure.
- The preservative solution is comprised of catechins and an organic acid. The catechins preferably comprise at least 25 wt % epigallocatechin-3-gallate (EGCG), and are preferably provided by green tea extract having a catechin content of at least 50 wt %, more preferably at least 75 wt %, and more particularly an epigallocatechin-3-gallate (EGCG) content of at least 20 wt %, more preferably at least 40 wt %. The green tea extract also preferably contains less than 25 wt % caffeine, more preferably less than 10 wt % caffeine. The organic acid content can be provided by one or more of ascorbic acid, erythorbic acid, or a salt thereof. Ascorbic acid and erythorbic acid have natural antioxidant properties, adding to the overall antioxidant potential of the preservative solution. The final, diluted preservative solution applied to produce in accordance with the invention contains at least 0.008 wt % catechins, and preferably contains 0.008% to 10% green tea extract catechins, more preferably at least 0.02%, at least 0.05%, or at least 0.08%, and more preferably at most 2%, at most 1%, or at most 0.5% green tea extract catechins. The final, diluted preservative solution preferably contains 0.2% to 20% organic acid or salt thereof, more preferably at least 0.5%, at least 1%, or at least 2%, and more preferably at most 10%, at most 7% or at most 5% organic acid or salt thereof. The weight ratio of catechins to organic acid or salt thereof is from 1:10 to 1:300, more preferably from about 1:100 to 1:300, as use of more than 300 parts organic acid to 1 part catechin is not needed to provide the observed preservative effect, and as use of less than 10 parts organic acid to 1 part catechin may not provide as great as desired effect. Unless otherwise stated, all percentages and component ratios further indicated herein are also weight (wt) percentages and weight (wt) ratios.
- If desired, the pH of the preservative solution can be increased with a buffering agent such that the pH of the final, diluted preservative solution is still acidic (i.e., <7.0). Adjusting the pH decreases the acidic taste of the preservative solution. This is important for preservative solutions with very high ascorbic acid concentrations; however, since the preservative solutions described in accordance with the invention enable relatively low ascorbic acid concentrations due to the synergistic interaction with the green tea extract catechins, neutralization may not be required. If raising the pH is desired, the buffering agent can be any basic neutralizing agent acceptable for use on food such as sodium hydroxide, potassium hydroxide, calcium carbonate, sodium carbonate, sodium bicarbonate, potassium carbonate, or potassium bicarbonate. Alternatively, a combination of the organic acid and any salt form of the organic acid can be combined in a ratio that results in the target pH.
- If antimicrobial properties are desired to further help extend the shelf-life of the treated produce, a food-safe anionic surfactant can be added to the formulation preferably at 0.001% to 0.5%, more preferably at least 0.02%, at least 0.04%, or at least 0.08%, and more preferably at most 0.2%, at most 0.15%, or at most 0.1%. Useful anionic surfactants include, e.g., sulfated fatty acids such as C6-C18 alkyl sulfates, more particularly C8-C14 alkyl sulfates, and in particular sodium lauryl sulfate.
- The preservative solution can be prepared by combining ingredients at final ready-to-use concentrations, or by diluting previously made concentrated solutions or powder mixtures with added water. For powder and concentrated solutions, the amount of green tea extract catechins, organic acid, and buffering agent in the powder or concentrated solution are increased in proportion to the final use concentrations. The use of concentrated solutions allow end users to prepare larger quantities of end-use solutions from small amounts of concentrated solution, which facilitates shipping and handling by minimizing product volume. In a particular embodiment, e.g., the concentrated solution compositions may be designed to be diluting with at least one, and more preferably at least 9 additional parts of water to one part of the concentrate composition to form a diluted end use solution. For example, if a 10× concentrated solution is prepared and the final, diluted solution is desired to provide 0.1% of green tea extract catechins and 3% of ascorbic acid, the concentration of green tea extract catechins in the 10× concentrated solution would be 1% and the concentration of ascorbic acid would be 30%. In the case of the powder or concentrated solution, the preservative powder or concentrate is diluted in normal processing water to the desired use concentration. The processed produce is then treated with the preservative solution by dipping, spraying, coating, or any other means that results in adequate exposure of the cut or peeled surface of the produce to the preservative solution. After treatment, the preserved produce may then be packaged and stored using conventional procedures and temperatures. In order to maximize the duration of preservation, it is preferred that the preserved produce is stored between −6° C. to 20° C., preferably, −3 to 10° C., and most preferably 0 to 6° C., under normal atmosphere content and pressure. When preserved and stored in this manner, even more perishable processed produce, such as apples and potatoes, are preserved in a natural state that retains color, texture, and flavor under normal refrigerated storage conditions.
- Examples of Preservative Solutions
- The following are examples, not meant to be all inclusive, of different preservative solutions. All components of the preservatives are food grade and allowed for use on food in the United States by the US Food and Drug Administration. The green tea extract (GTE) employed in all examples comprised 83.2% total catechins, and more particularly 50.1% EGCG, and 1.8% caffeine. In the examples, water can be eliminated from each example to provide a concentrated powder. For concentrated powders, the ready-to-use liquid antibrowning solution is prepared by adding the indicated amount of water to the indicated total weight of powder.
- A ready-to-use solution is prepared by combining the following in water:
- 1. 10 g of ascorbic acid
- 2. 1 g of green tea extract (83% catechins)
- 3. Water to 1 L
- A ready-to-use solution is prepared by combining the following in water:
- 1. 30 g of ascorbic acid
- 2. 1 g of green tea extract (83% catechins)
- 3. Water to 1 L
- A ready-to-use solution is prepared by combining the following in water:
- 1. 30 g of ascorbic acid
- 2. 15 g of sodium bicarbonate
- 3. 1 g of green tea extract (83% catechins)
- 4. Water to 1 L
- A 5-fold concentrated solution is prepared by combining the following in water:
- 1. 200 g of ascorbic acid
- 2. 25 g of calcium carbonate
- 3. 15 g of green tea extract (83% catechins)
- 4. Water to 1 L
- A ready-to-use solution is prepared by combining the following in water:
- 1. 40 g of erythorbic acid
- 2. 2 g of calcium carbonate
- 3. 10 g of sodium bicarbonate
- 4. 3 g of green tea extract (83% catechins)
- 5. Water to 1 L
- A 10-fold concentrated solution is prepared by combining the following in water:
- 1. 300 g of ascorbic acid
- 2. 30 g of calcium carbonate
- 3. 100 g of sodium bicarbonate
- 4. 10 g of green tea extract (83% catechins)
- 5. Water to 1 L
- A ready-to-use solution is prepared by combining the following in water:
- 1. 40 g of calcium ascorbate
- 2. 1 g of green tea extract (83% catechins)
- 3. Water to 1 L
- A ready-to-use solution is prepared by combining the following in water:
- 1. 30 g of ascorbic acid
- 2. 1 g of green tea extract (83% catechins)
- 3. 0.8 g of sodium lauryl sulfate
- 4. Water to 1 L
- A concentrated powder is prepared by combining the following ingredients. When used to prepare the final antibrowning solution, 31.4 g of powder is added to 1 liter of water:
- 1. 30 g of ascorbic acid
- 2. 1 g of green tea extract (83% catechins)
- 3. 0.4 g of sodium lauryl sulfate
- A ready-to-use solution is prepared by combining the following in water:
- 1. 30 g of ascorbic acid
- 2. 1 g of green tea extract (83% catechins)
- 3. 0.2 g of sodium lauryl sulfate
- 4. Water to 1 L
- Assessment of the Performance of the Preservative Solutions
- The effectiveness of the preservation solution on one highly perishable fruit, apple slices, and one highly perishable vegetable, potato slices, was evaluated by a battery of tests.
- Preliminary testing was conducted with aqueous solutions of various concentrations of ascorbic acid, green tea extract, and combinations of ascorbic acid and green tea extract as noted in Table 1 below to determine the ability of ascorbic acid to prevent the brown discoloration associated with green tea extract catechins, and determine the optimal concentrations of combinations of ascorbic acid and green tea extract catechins for preventing the browning of red delicious apple slices. A description of the qualitative and quantitative color assessment procedures is provided below.
-
TABLE 1 Qualitative and quantitative assessments of color of red delicious apple slices during storage at 4° C. Time of Quantitative Assessment Time of Blue Value White Value Qualitative Assessment (% of (% of Color Fresh Cut) Fresh Cut) Treatment 5 minutes 1 day 2 days 2 days 2 days Untreated W +++ B +++ B 64.0 79.4 0.003% GTE W +++ B +++ B NC NC 0.01% GTE +/− B + B + B NC NC 0.03% GTE +/− B + B + B NC NC 0.1% GTE +B + B + B NC NC 3% AA W +/− B + B 72.6 83.1 3% AA + W +/− B + B 79.0 85.7 0.003% GTE 3% AA + W W W 96.7 95.0 0.01% GTE 3% AA + W W W 98.0 98.1 0.03% GTE 3% AA + W W W 97.2 97.5 0.1% GTE NOTES: GTE = green tea extract (83% catechins), AA = ascorbic acid, B = brown color, W = white color (same as fresh cut slices), NC = not conducted. Brown discoloration scored on the following scale: W = no browning, +/− = very slight, + = slight, ++ = minimal, +++ = moderate, ++++ = marked. n = 4 slices per treatment. - Green tea extract at 0.003% (i.e., approximately 0.0025% catechins) did not discolor the flesh of the apple slices; however, it also did not provide a synergistic interaction with ascorbic acid in enhancing the anti-browning effect (Table 1). In contrast, green tea extract at 0.01% (i.e., C approximately 0.008% catechins) stained the flesh of apple slices brown; however, the addition of 3% ascorbic acid prevented this brown staining (Table 1). In addition, green tea extract at −0.01% had a strong synergistic interaction with 3% ascorbic acid and maintained the appearance of the apple slices similar to the white flesh of fresh cut apple slices over 2 days storage at 4° C. (Table 1). In subsequent experiments, the preservation effect was tested for longer durations as outlined below.
- Color was assessed either by a qualitative rating by eye or quantitatively using a digital color scanner after the specified period of storage. For quantitative assessment, the cut surface of each apple or potato slice was digitized as a color bitmap image at a resolution of 200 dots per inch using 16-bit red, green, blue (RGB) color. In order to ensure the images were standardized, a series of white, several gradations of grey, and black color swatches were scanned with each scan. The average individual RGB signals were measured for each apple or potato slice using the publicly available program NIH Image J 1.47v (available at imagej.nih.gov/ij). Whiteness was assessed as the average of the red+green+blue values. In addition, since decreases in the blue signal are highly associated with the color of browning observed in processed apples and potatoes, the changes in the blue signal were assessed individually. Decreases in the red and green signals are also associated with browning; however, they are typically less affected than the blue signal, making changes in the blue signal a good surrogate for browning of produce. For each time point and treatment, at least four apple or potato slices were measured and the values averaged.
- For the apples slices, the flavor, texture, and moistness of the preserved apple slices were qualitatively assessed by tasting the slices after 14 days of storage and comparing them to fresh cut apple slices. For the apple and potato slices, color and appearance were also rated qualitatively and compared to fresh cut apples or potatoes on each assessment day. Firmness of the apple slices was assessed after 14 days of storage using a manual penetrometer and compared to fresh cut apple slices.
- A series of tests were conducted to determine the efficacy of the preservative solutions on apple and potato slices.
- In the first test, the following ready-to-use preservative solutions were prepared in water:
- 1) 3% ascorbic acid+0.1% green tea extract (83% catechins)
- 2) 1% ascorbic acid+0.1% green tea extract (83% catechins)
- Red delicious apples were washed thoroughly with soap and water and rinsed thoroughly with water after washing. Red delicious apples were selected since they are one of the most difficult apple varieties to prevent from browning. Apples slices were prepared by using a manual apple corer/slicer that prepared 10 slices per apple. Apple slices were randomly mixed from multiple apples and then placed in each preservative solution, 1 or 3% ascorbic acid, 0.1% green tea extract (83% catechins), or left untreated for approximately 5 minutes. The slices were then placed in plastic containers and stored at approximately 4° C. After the indicated time, one cut surface of each slice was digitized. In addition, the color, general appearance, and smell were assessed qualitatively at the indicated times and compared to fresh cut apple slices. After 14 days of storage, apple slices were assessed for firmness quantitatively using a manual penetrometer. After 14 days of storage, flavor, moisture, and texture were qualitatively assessed only for slices that had normal appearance by tasting each slice.
-
TABLE 2 Quantitative measurement of blue and white values of cut surface of red delicious apple slices during storage at 4° C. Day of Assessment Blue Value White Value (% of Fresh Cut) (% of Fresh Cut) Treatment 1 7 14 1 7 14 Untreated 82.6 69.1 67.3 90.1 81.6 80.6 0.1% GTE 81.8 68.0 65.7 87.8 77.6 76.3 1% AA 82.9 74.5 64.7 87.2 82.9 77.1 3% AA 97.3 84.1 79.2 94.9 88.1 86.7 1% AA + 97.6 84.5 73.4 95.3 87.9 80.8 0.1% GTE 3% AA + 100.6 101.3 100.4 96.2 97.0 96.5 0.1% GTE NOTES: GTE = green tea extract (83% catechins), AA = ascorbic acid, blue value = blue channel value from digitized image of cut surface of apple slice, white value = average of red + green + blue (RGB) values from digitized image of cut surface of apple slice. n = 4 slices per treatment. - The data in Table 2 demonstrate that the combination of 3% ascorbic acid and 0.1% green tea extract (i.e., approximately 0.08% catechins) prevented the residual self-staining inherent with green tea extract catechins and potentiated the antibrowning properties of either component by itself.
-
TABLE 3 Firmness measurement of red delicious apple slices after 14 days of storage at 4° C. Treatment Firmness (% of Fresh Cut) Untreated 76.5 0.1% GTE 86.0 1% AA 86.5 3% AA 85.5 1% AA + 0.1% GTE 95.0 3% AA + 0.1% GTE 102.5 NOTES: GTE = green tea extract (83% catechins), AA = ascorbic acid. n = 5 slices per treatment. - The data in Table 3 demonstrate that the combination of 3% ascorbic acid and 0.1% green tea extract (i.e., approximately 0.08% catechins) maintained the firmness of the apple slices during storage.
-
TABLE 4 Qualitative assessments of color and smell of red delicious apples slices during storage at 4° C. Time of Assessment 4 hours 1 day 3 days 7 days 14 days Treatment Color Smell Color Smell Color Smell Color Smell Color Smell Untreated +++ B Fresh +++ B None +++ B None +++ B None ++++ B Fermented 0.1% GTE + B Fresh + B Fresh + B Minimal ++ B Minimal ++++ B Minimal 1% AA ++ B Fresh ++ B Fresh ++ B Minimal ++ B Minimal +++ B Minimal 3% AA W Fresh W Fresh +/− B Fresh + B Minimal ++ B Minimal 1% AA + W Fresh W Fresh +/− B Fresh + B Minimal ++ B Weak 0.1% GTE 3% AA + W Fresh W Fresh W Fresh W Fresh W Fresh 0.1% GTE NOTES: GTE = green tea extract (83% catechins), AA = ascorbic acid, B = brown color, W = white color. Brown discoloration scored on the following scale: W = no browning, +/− = very slight, + = slight, ++ = minimal, +++ = moderate, ++++ = marked. n = 4 slices per treatment. Smell assessed as follows: fresh = smell of fresh cut apple slices, minimal = no significant smell, weak = slight fresh cut apple slice smell, fermented = noticeable fermented smell. -
TABLE 5 Qualitative assessments of flavor, moistness, and texture of red delicious apple slices after 14 days of storage at 4° C. Treatment Flavor Moistness Texture Untreated Not tested Not tested Not tested 0.1% GTE Not tested Not tested Not tested 1% AA Not tested Not tested Not tested 3% AA Not tested Not tested Not tested 1% AA + Not tested Not tested Not tested 0.1% GTE 3% AA + Same as Same as Same as 0.1% GTE fresh cut slices fresh cut slices fresh cut slices NOTES: GTE = green tea extract (83% catechins), AA = ascorbic acid. Samples labeled as “not tested” were not assessed since they exhibited significant browning and were not considered palatable at the assessment time point. - The data in Tables 4 and 5 demonstrate that the combination of 3% ascorbic acid and 0.1% green tea extract (i.e., approximately 0.08% catechins) maintained the color, smell, flavor, moistness, and texture of apple slices.
- In order to test the effect of a pH modifier on the efficacy of the preservative solution, the following Ready-to-use preservative solutions were prepared in water:
-
- 1) 3% ascorbic acid+0.1% green tea extract (83% catechins)
- 2) 3% ascorbic acid+1.33% sodium bicarbonate+0.1% green tea extract (83% catechins)
- 3) 3% ascorbic acid+1.5% sodium bicarbonate+0.1% green tea extract (83% catechins)
- Red delicious apples were washed thoroughly with soap and water and rinsed thoroughly with water after washing. Apples slices were prepared by using a manual apple corer/slicer that prepared 10 slices per apple. Apple slices were randomly mixed from multiple apples and then placed in each preservative solution or 3% ascorbic acid for approximately 5 minutes. The slices were then placed in plastic containers and stored at approximately 4° C. After the indicated time, the slices were qualitatively assessed for appearance.
-
TABLE 6 Qualitative assessment of appearance of red delicious apple slices in various preservative solutions after storage at 4° C. Appearance Treatment pH of Solution Day 2 Day 7 Day 14 3% AA 2.25 W + B ++ B 3% AA + 0.1% GTE 2.31 W W W 3% AA + 1.33% 5.42 W W W SB + 0.1% GTE 3% AA + 1.5% 6.27 W W W SB + 0.1% GTE NOTES: GTE = green tea extract (83% catechins), AA = ascorbic acid, SB = sodium bicarbonate, B = brown color, W = white color. Brown discoloration scored on the following scale: W = no browning, +/− = very slight, + = slight, ++ = minimal, +++ = moderate, ++++ = marked. n = 4 slices per treatment. - The data in Table 6 demonstrate that the efficacy of the ascorbic acid and green tea extract catechins preservative solutions was the same whether or not sodium bicarbonate was added to increase the pH of the final preservative solution.
- In order to determine if the preservative solution would work equally as well on completely peeled, cored, and sliced apples, the following ready-to-use preservative solution was prepared in water:
-
3% ascorbic acid+0.1% green tea extract (83% catechins) - Red delicious apples were washed thoroughly with soap and water and rinsed thoroughly with water after washing. The apples were manually peeled and then slices prepared using a manual apple corer/slicer that prepared 10 slices per apple. Apple slices were randomly mixed from multiple apples and then placed in the preservative solution or 3% ascorbic acid for approximately 5 minutes. The slices were then placed in a plastic container and stored at approximately 4° C. After the indicated time, the entire slice (peeled and cut surfaces) was qualitatively assessed for appearance.
-
TABLE 7 Qualitative assessment of appearance of peeled red delicious apple slices after storage at 4° C. Appearance Treatment 6 Hours 1 Day 2 Days 7 Days 14 Days Untreated ++ B +++ B +++ B Not assessed Not assessed 3% AA W + B + B ++ B ++ B 3% AA + W W W W W 0.1% GTE NOTES: GTE = green tea extract (83% catechins), AA = ascorbic acid, B = brown color, W = white color. Brown discoloration scored on the following scale: W = no browning, +/− = very slight, + = slight, ++ = minimal, +++ = moderate, ++++ = marked. n = 4 slices per treatment. - The data in Table 7 demonstrate that the 3% ascorbic acid and 0.1% green tea extract (i.e., approximately 0.08% catechins) preservative solution maintained the whiteness of the peeled and cut surfaces of the peeled apple slices.
- The efficacy of the preservative solution was tested on baking potatoes, which are a difficult vegetable to prevent from browning. The following ready-to-use preservative solutions were prepared in water:
-
4% ascorbic acid+0.1% green tea extract (83% catechins) - Baking potatoes were washed thoroughly with soap and water and rinsed thoroughly with water after washing. The potatoes were peeled and then rinsed with water. The potato slices were prepared by manual slicing with a knife. Slices were randomly mixed from multiple potatoes and then placed in preservative solution, 4% ascorbic acid, or 0.1% green tea extract (83% catechins) for approximately 5 minutes. The slices were then placed in plastic containers and stored at approximately 4° C. After the indicated time, one cut surface of each slice was digitized. In addition, the color and general appearance were assessed qualitatively at the indicated times and compared to fresh cut potato slices.
-
TABLE 8 Quantitative measurement of blue and white values of cut surface of potato slices during storage at 4° C. Day of Assessment Blue Value (% of Fresh Cut) White Value (% of Fresh Cut) Treatment 4 6 7 4 6 7 Untreated 44.3 53.4 56.2 51.3 59.6 61.0 0.1% GTE 83.6 80.2 78.2 87.3 84.4 82.3 4% AA 92.0 88.5 87.2 93.7 93.0 92.1 4% AA + 97.4 97.2 96.6 96.5 96.2 96.6 0.1% GTE NOTES: GTE = green tea extract (83% catechins), AA = ascorbic acid, blue value = blue channel value from digitized image of cut surface of apple slice, white value = average of red + green + blue (RGB) values from digitized image of cut surface of apple slice. n = 4 slices per treatment. - The data in Table 8 demonstrate that the combination of 4% ascorbic acid and 0.1% green tea extract (i.e., approximately 0.08% catechins) potentiated the antibrowning properties of either component by itself.
-
TABLE 9 Qualitative assessment of color and appearance of peeled baking potato slices after storage at 4° C. Color and Appearance Treatment 1 Day 3 Days 4 Days 5 Days 6 Days 7 Days Untreated +++ B & G ++++ B & G ++++ B & G ++++ B & G ++++ B & G ++++ B & G Dry Dry Dry Dry Dry appearance appearance appearance appearance appearance 0.1% GTE +/− B + B + B ++ B ++ B ++ B Dry Dry Dry appearance appearance appearance 4% AA W +/− B + B + B ++ B ++ B Dry Dry Dry Dry appearance appearance appearance appearance 4% AA + W W W W +/− B +/− B 0.1% GTE NOTES: GTE = green tea extract (83% catechins), AA = ascorbic acid, B = brown color, G = grey color, W = white color. Dry appearance = no moistness on assessed surface and dried and cracking lines/areas on surface. Discoloration scored on the following scale: W = no discoloration, +/− = very slight, + = slight, ++ = minimal, +++ = moderate, ++++ = marked, n = 4 slices per treatment. Color and appearance labeled as just “W” exhibited a color and appearance the same as fresh cut potato slices. - The data in Table 9 demonstrate that the 4% ascorbic acid and 0.1% green tea extract (i.e., approximately 0.08% catechins) preservative solution maintained the whiteness of the peeled and cut surfaces of the potato slices better than ascorbic acid or green tea extract alone.
- An antibrowning solution with improved antimicrobial properties can be prepared by adding a food-safe anionic surfactant such as sodium lauryl sulfate. The antimicrobial properties of the three solutions from Examples 8, 9, and 10 was assessed against a yeast that is problematic in food processing operations, Candida albicans. The effectiveness was assessed in a suspension time kill assay. In brief, the microbial suspension (1×107 CFU/ml) was added to 9.9 times the volume of 1× end-use antibrowning/antimicrobial solution. After 15 minutes, an aliquot of the test solution was removed and neutralized with culture broth. The neutralized solution containing yeast was then plated on solid agar medium and allowed to incubate at 36±1° C. for 48 hours to identify viable colonies. Table 10 summarizes the results of duplicate replicates including the log-fold reduction in yeast for the two replicates combined for each solution. All example formulations containing the anionic surfactant (Examples 8, 9, and 10) provided dramatic reductions in yeast counts with no surviving yeast detected after treatment with the solutions. The solution from Example 2 (no anionic surfactant) was included to demonstrate the importance of adding the anionic surfactant for achieving improved antimicrobial properties, as the solution in Example 2 provided only a minimal reduction of yeast counts.
-
TABLE 10 Antimicrobial efficacy of example antibrowning/antimicrobial solutions AVERAGE ANTIBROWNING/ INITIAL YEAST VIABLE LOG10 ANTIBACTERIAL EXPOSURE CONCNTRTN. COLONIES REDUCTION SOLUTION ORGANISM REPLICATE TIME (MIN) (CFU/ML) (CFU/ML) IN BACTERIA Example 2 Candida 1 15 7.34 × 106 4.30 × 106 0.35 albicans 2 2.30 × 106 Example 8 Candida 1 15 7.34 × 106 No growth >6.17 albicans 2 No growth Example 9 Candida 1 15 7.34 × 106 No growth >6.17 albicans 2 No growth Example 10 Candida 1 15 7.34 × 106 No growth >6.17 albicans 2 No growth - While the invention has been described in connection with several presently preferred embodiments thereof, those skilled in the art will appreciate that many modifications and changes may be made without departing from the true spirit and scope of the invention which accordingly is intended to be defined solely by the appended claims.
Claims (35)
1. A method of preserving fruits and vegetables comprising:
a. providing an aqueous preservative solution comprising (i) at least 0.008 wt percent catechins and (ii) an organic acid or salt thereof selected from any one or any combination of ascorbic acid, erythorbic acid, and salts thereof; and
b. applying the preservative solution to a fruit or vegetable.
2. The method of claim 1 wherein the catechins in the preservative solution are provided by a green tea extract.
3. The method of claim 2 wherein the green tea extract content in the preservative solution is from about 0.01 to 10% by weight.
4. The method of claim 3 wherein the green tea extract comprises at least 20 wt % epigallocatechin-3-gallate.
5. The method of claim 4 wherein the green tea extract comprises less than 10% caffeine.
6. The method of claim 1 , further comprising packaging the fruit or vegetable after applying the preservative solution.
7. The method of claim 6 , further comprising storing the packaged fruit or vegetable at from about −6° C. to about 20° C. under normal atmosphere and pressure.
8. The method of claim 6 wherein the fruit or vegetable is stored at from about 0° C. to about 6° C. under normal atmosphere and pressure.
9. The method of claim 1 wherein the catechins comprise at least 25 wt % epigallocatechin-3-gallate.
10. The method of claim 1 wherein the preservative solution further comprises a basic neutralizer selected from the group consisting of: sodium hydroxide, potassium hydroxide, sodium carbonate, sodium bicarbonate, potassium carbonate, potassium bicarbonate, and calcium carbonate.
11. The method of claim 1 wherein the preservative solution comprises ascorbic acid or erythorbic acid, and a salt of ascorbic acid or of erythorbic acid.
12. The method of claim 1 wherein the organic acid or salt thereof is present in the preservative solution at from 0.2 wt % to 20 wt %.
13. The method of claim 1 wherein the organic acid or salt thereof is present in the preservative solution at from 0.5 wt % to 10 wt %.
14. The method of claim 1 wherein the organic acid or salt thereof is present in the preservative solution at from 1 wt % to 7 wt %.
15. The method of claim 1 wherein the organic acid or salt thereof is present in the preservative solution at from 2 wt % to 5 wt %.
16. The method of claim 1 wherein the preservative solution contains 0.008 wt % to 10 wt % catechins.
17. The method of claim 1 wherein the preservative solution contains 0.02 wt % to 1 wt % catechins.
18. The method of claim 1 wherein the preservative solution contains 0.05 wt % to 0.5 wt % catechins and the organic acid or salt thereof is present in the preservative solution at from 2 wt % to 5 wt %.
19. The method of claim 1 wherein the organic acid or salt thereof is ascorbic acid or salt thereof.
20. The method of claim 1 wherein the preservative solution further comprises an anionic surfactant present at 0.001 wt % to 0.5 wt %.
21. The method of claim 20 wherein the anionic surfactant is sodium lauryl sulfate.
22. An aqueous solution comprising
(i) at least 0.008 wt percent catechins; and
(ii) an organic acid or salt thereof selected from any one or any combination of ascorbic acid, erythorbic acid, and salts thereof.
23. The aqueous solution of claim 22 , wherein all or a portion of the catechins comprise epigallocatechin-3-gallate.
24. The aqueous solution of claim 23 , wherein the catechins comprise at least 25 wt % epigallocatechin-3-gallate.
25. The aqueous solution of claim 22 further comprising a basic neutralizer selected from the group consisting of: sodium hydroxide, potassium hydroxide, sodium carbonate, sodium bicarbonate, potassium carbonate, potassium bicarbonate, and calcium carbonate.
26. The aqueous solution of claim 22 , wherein the organic acid comprises calcium ascorbate.
27. The aqueous solution of claim 26 , wherein the catechins comprise catechins derived from green tea extract.
28. The aqueous solution of claim 22 , wherein the catechins are present in an amount ranging from 0.008 wt % to 10 wt %.
29. The aqueous solution of claim 28 , wherein the catechins are present in an amount ranging from 0.02 wt % to 1 wt %.
30. The aqueous solution of claim 22 , wherein the catechins are present in an amount ranging from 0.05 wt % to 0.5 wt % and the organic acid or salt thereof is present in the preservative solution at from 2 wt % to 5 wt %.
31. The aqueous solution of claim 22 further comprising an antimicrobial agent.
32. The aqueous solution of claim 31 , wherein the antimicrobial agent comprises sodium lauryl sulfate.
33. A kit for preparing a solution for preserving fruits and vegetables comprising:
catechins;
an organic acid or salt thereof selected from any one or any combination of ascorbic acid, erythorbic acid, and salts thereof; and
instructions for mixing a quantity of the catechins together with a quantity of the organic acid or salt thereof and water to prepare a solution having at least 0.008 wt percent catechins.
34. The kit of claim 33 wherein the catechins are contained in a first container and the organic acid is contained in a second container.
35. The kit of claim 33 wherein the organic acid is calcium ascorbate.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US15/217,117 US20160338368A1 (en) | 2015-02-13 | 2016-07-22 | Fruit and vegetable preservative |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US14/621,793 US9426998B1 (en) | 2015-02-13 | 2015-02-13 | Fruit and vegetable preservative |
US15/217,117 US20160338368A1 (en) | 2015-02-13 | 2016-07-22 | Fruit and vegetable preservative |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US14/621,793 Continuation US9426998B1 (en) | 2015-02-13 | 2015-02-13 | Fruit and vegetable preservative |
Publications (1)
Publication Number | Publication Date |
---|---|
US20160338368A1 true US20160338368A1 (en) | 2016-11-24 |
Family
ID=56615745
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US14/621,793 Expired - Fee Related US9426998B1 (en) | 2015-02-13 | 2015-02-13 | Fruit and vegetable preservative |
US15/217,117 Abandoned US20160338368A1 (en) | 2015-02-13 | 2016-07-22 | Fruit and vegetable preservative |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US14/621,793 Expired - Fee Related US9426998B1 (en) | 2015-02-13 | 2015-02-13 | Fruit and vegetable preservative |
Country Status (7)
Country | Link |
---|---|
US (2) | US9426998B1 (en) |
EP (1) | EP3253221A4 (en) |
JP (1) | JP2018504938A (en) |
CN (1) | CN107427016A (en) |
BR (1) | BR112017017271A2 (en) |
HK (1) | HK1244631A1 (en) |
WO (1) | WO2016130922A2 (en) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
PL3338563T3 (en) * | 2016-12-21 | 2019-09-30 | Benjamin Amit Singh | Method for preserving fresh produce, solid composition for application in a method for preserving fresh produce and preserved fresh produce |
CN109287739A (en) * | 2018-10-12 | 2019-02-01 | 绩溪县桐坑源家庭农场 | A kind of safe and reliable agent keeping vegetable fresh and preparation method thereof |
CA3150533A1 (en) * | 2019-09-27 | 2021-04-01 | Darrin Dale WINGER | Fry assessment system and method |
WO2021097025A1 (en) * | 2019-11-15 | 2021-05-20 | Dupont Nutrition Biosciences Aps | Process for reducing oxidation of a foodstuff and related compositions |
CN111011480A (en) * | 2019-12-12 | 2020-04-17 | 南昌大学 | Rambutan special-effect preservative and using method thereof |
DE102020003111B4 (en) * | 2020-05-23 | 2021-12-30 | Die Frischemanufaktur GmbH | Process for the production of a beverage filled into a container and containing at least one physical piece of at least one flavor carrier for human consumption, immersion bath solution for use in the process and beverage product |
CN112640950A (en) * | 2020-12-15 | 2021-04-13 | 四川东坡中国泡菜产业技术研究院 | Method for prolonging shelf life of pickled vegetables by combining low-temperature-resistant lactic acid bacteria and catechin |
CN113303421B (en) * | 2021-06-16 | 2022-04-26 | 中国农业大学 | Method for inhibiting PME (polymethylene oxide) and/or PPO (polyphenylene oxide) activity in fruit and vegetable products by ultrahigh pressure synergistic EGCG (epigallocatechin gallate) |
WO2023128771A1 (en) * | 2021-12-30 | 2023-07-06 | Bio Natural Solutions S.A.C. | Method for extracting flavonols derived from tropical fruit waste to preserve fresh and cut foods, and composition comprising same |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20070141219A1 (en) * | 2003-12-18 | 2007-06-21 | Kao Corporation | Bottle beverage |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4344971A (en) * | 1980-10-09 | 1982-08-17 | Grain Processing Corporation | Preservation of fruits and vegetables |
CA2159465A1 (en) * | 1993-04-01 | 1994-10-13 | Paul H. Todd, Jr. | Lipid-soluble green tea catechin antioxidant solutions |
AUPQ598300A0 (en) * | 2000-03-03 | 2000-03-23 | Citrus Sensation Pty Ltd | Citrus fruit preservative |
JP2006188436A (en) * | 2004-12-28 | 2006-07-20 | Japan Science & Technology Agency | Medical polyphenol solution |
JP4604195B2 (en) * | 2005-01-12 | 2010-12-22 | 藤田 殉子 | Peeling cored cut apple freshness retention solution, freshness retention method and freshness retention package |
JP5087152B2 (en) * | 2011-03-25 | 2012-11-28 | ソルジェンテ株式会社 | Freshness preservation method for fresh food |
-
2015
- 2015-02-13 US US14/621,793 patent/US9426998B1/en not_active Expired - Fee Related
-
2016
- 2016-02-12 JP JP2017560894A patent/JP2018504938A/en active Pending
- 2016-02-12 EP EP16749960.7A patent/EP3253221A4/en not_active Withdrawn
- 2016-02-12 WO PCT/US2016/017756 patent/WO2016130922A2/en active Application Filing
- 2016-02-12 BR BR112017017271-2A patent/BR112017017271A2/en not_active Application Discontinuation
- 2016-02-12 CN CN201680017257.5A patent/CN107427016A/en active Pending
- 2016-07-22 US US15/217,117 patent/US20160338368A1/en not_active Abandoned
-
2018
- 2018-03-06 HK HK18103202.8A patent/HK1244631A1/en unknown
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20070141219A1 (en) * | 2003-12-18 | 2007-06-21 | Kao Corporation | Bottle beverage |
Also Published As
Publication number | Publication date |
---|---|
US20160235081A1 (en) | 2016-08-18 |
BR112017017271A2 (en) | 2018-04-17 |
WO2016130922A3 (en) | 2016-11-24 |
CN107427016A (en) | 2017-12-01 |
HK1244631A1 (en) | 2018-08-17 |
JP2018504938A (en) | 2018-02-22 |
EP3253221A2 (en) | 2017-12-13 |
WO2016130922A2 (en) | 2016-08-18 |
EP3253221A4 (en) | 2018-04-04 |
US9426998B1 (en) | 2016-08-30 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US9426998B1 (en) | Fruit and vegetable preservative | |
Xiao et al. | Combined action of pure oxygen pretreatment and chitosan coating incorporated with rosemary extracts on the quality of fresh-cut pears | |
Oms-Oliu et al. | Recent approaches using chemical treatments to preserve quality of fresh-cut fruit: A review | |
Soliva-Fortuny et al. | New advances in extending the shelf-life of fresh-cut fruits: a review | |
US7198811B2 (en) | Compositions for the preservation of fruits and vegetables | |
US20020054950A1 (en) | Fruit and vegetable preservative | |
DE69317182T2 (en) | METHOD FOR STORING FOODSTUFFS WITH NOBLE GASES | |
US8512781B2 (en) | Fresh fruit preservative and method of using same | |
EP3092903B1 (en) | Edible coating for preserving pieces of fruit, production method and application thereof | |
Nasution et al. | Characteristics of fresh-cut guava coated with aloe vera gel as affected by different additives | |
JP2018505678A (en) | Antimicrobial composition | |
Saleh et al. | Effects of anti-coloring agents on blackening inhibition and maintaining physical and chemical quality of fresh-cut okra during storage | |
KR20140114819A (en) | Composition for extending the storage life of fresh produce | |
US20070184165A1 (en) | Compositions and methods for improving the efficiency of preservatives in foods | |
US5895681A (en) | Method of preserving tea containing beverages | |
US8883238B2 (en) | Fresh fruit preservative and method of using same | |
Colás Medà et al. | Development of a fresh-cut product based on pears and the subsequent evaluation of its shelf life under commercial conditions and after a cold chain break | |
US20150201633A1 (en) | Agent for preserving quality of peeled and/or cut vegetable or fruit and/or seafood | |
Devlieghere et al. | Modified atmosphere packaging (MAP) | |
Plotto et al. | Edible coatings and other surface treatments to maintain color of lychee fruit in storage. | |
KR102440111B1 (en) | Method for preventing browning reaction using a composition for prevnting browning reaction of fresh-cut produce | |
EP4009785B1 (en) | Method for preserving fresh food | |
JP2000342170A (en) | Food preservative | |
Wang et al. | Application of chitosan-gelatin active film with ethyl pyruvate on quality of fresh-cut pitaya fruit | |
US20210235729A1 (en) | Method For Treating The Surface Of Plant Parts |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
STCB | Information on status: application discontinuation |
Free format text: ABANDONED -- FAILURE TO PAY ISSUE FEE |