US20150182518A1

US20150182518A1 - Cancer cell inhibitory drug and cancer stem-cell detection probe

Info

Publication number: US20150182518A1
Application number: US14/408,787
Authority: US
Inventors: Taichi Shintou; Tsuyoshi Nomoto; Kohei Watanabe; Takeshi Miyazaki; Toshio Tanaka; Yasuhito Shimada; Yuhei Nishimura
Original assignee: Canon Inc
Current assignee: Canon Inc
Priority date: 2012-10-26
Filing date: 2013-10-25
Publication date: 2015-07-02
Also published as: JP2014101358A; WO2014065439A1; EP2849743A4; EP2849743A1; US10278965B2; EP2849743B1; US20170340622A1; JP6384821B2

Abstract

An object of the present invention is to provide a cancer cell inhibitory drug, particularly a cancer stem-cell inhibitory drug, or a cancer stem-cell detection probe. The present invention provides a cancer cell inhibitory drug comprising at least one compound represented by general formula (1) as an active ingredient.

Description

TECHNICAL FIELD

The present invention relates to a cancer cell inhibitory drug, particularly to a cancer stem cell inhibitory drug, and a cancer stem-cell detection probe.

BACKGROUND ART

At present, as a general cancer therapy, e.g., radiation therapy, chemotherapy, immunotherapy and surgical (excision) therapy are mentioned. The chemotherapy is a method for suppressing cancer by use of an anticancer therapeutic agent made of various types of low-molecular compounds.
The therapy using an anticancer therapeutic agent is directed to reduce the size of a solid tumor. However, the most part of a tumor is occupied by differentiated cancer cells which no longer have a function as a cancer stem cell and it is pointed out in a general anticancer agent treatment that the differentiated cancer cells are only targeted to reduce the size thereof.
Cancer has cells having nature of stem cells, called cancer stem cells.
The cancer stem cells, which were first identified in 1997 in an acute myeloid leukemia, are now increasingly found in various types of cancers including solid cancers, and recently, a new way of thinking, called “cancer stem cell hypothesis” that cancer would be developed from cancer stem cells as an origin, has been proposed (NPL 1).
According to the hypothesis, even though the most part of cancer cells are killed or excised out by applying the aforementioned therapy, if a very small number of self-reproducible cancer stem cells remain, recurrence and metastasis conceivably occur. In short, it is considered that recurrence and metastasis are caused by the remaining small amount of cancer stem cells. Accordingly, if cancer stem cells can be targeted and completely eradicated, it is expected to develop a useful therapy for preventing metastasis and recurrence of cancer.
It is pointed out that some of the cancer stem cells acquire drug resistance to an anticancer therapeutic agent (NPL 2).
At present, as a low-molecular compound for use in detection of cancer stem cells and as a therapeutic agent, a compound containing radioactive Cu-ATSM is known (PTL 1). However, the radioactive compound may affect normal cells. Therefore, when a radioactive compound is used, safety becomes a matter of concern. In addition, it is also pointed out that cancer stem cells may develop strong resistance to radiation.
In the circumstances, it has been desired to develop a drug inhibiting cancer stem cells and a compound capable of detecting cancer stem cells.

CITATION LIST

Patent Literature

PTL 1: Japanese Patent Application Laid-Open No. 2010-013380

Non Patent Literature

NPL 1: Carcinogenesis, Vol. 26, p.p. 703-711, 2005
NPL 2: Nature Review Cancer., Vol. 5, p.p. 275-284, 2005
NPL 3: Journal of the American Chemical Society, Vol. 133, p.p. 6626-6635, 2011
NPL 4: Dye and Pigments, Vol. 71, p.p. 28-36, 2005
NPL 5: Organic & Biomolecular Chemistry, Vol. 9, p.p. 4199-4204, 2011

SUMMARY OF INVENTION

Technical Problem

Cancer stem cells have high resistance to radiation therapies and chemotherapies conventionally used and are casual cells from which cancer growth, recurrence and metastasis occur. Up to present, where cancer stem cells are present cannot be clearly detected. This was a issue remaining unsolved. To completely cure cancer, it has been strongly desired to detect cancer stem cells and develop a drug inhibiting cancer cells, in particular, cancer stem cells.

Solution to Problem

The present inventors intensively made studies with a view to solving the aforementioned problem. As a result, they found that a compound represented by the following general formula (1) has an inhibitory effect on cancer cells and is selectively taken into particularly cancer stem cells among the cancer cells and inhibits them. Based on the finding, the present invention was accomplished.
Furthermore, the compound of the present invention has a luminescence property. Owing to this, the position of cancer cells can be identified (determined) by detecting luminescence of the compound selectively taken into cancer cells. Based on the finding, the present inventors arrived at the present invention. Note that, in the specification, luminescence includes fluorescence and phosphorescence. Since the compound of the present invention is taken into particularly cancer stem cells in a high ratio, cancer stem cells can be selectively detected.
More specifically, the compound of the present invention contains a compound represented by general formula (1):
In general formula (1), R₁and R₂each independently represent an alkyl group, a carboxylalkyl group, an alkoxycarbonylalkyl group or an alkylcarbonyloxyalkyl group; R₃to R₁₀each independently represent a hydrogen atom, an alkyl group, an aryl group, an alkoxy group, a halogen atom, an alkoxysulfonyl group, a N-alkylsulfamoyl group, an alkyloxycarbonyl group, a carbamoyl group or a N-alkylcarbamoyl group; R₃and R₄, R₅and R₆, R₇and R₈, and R₉and R₁₀may be each independently cyclized to form a benzene ring; X₁ ⁻ represents an anionic group; Y₁is a group including *1, *2 and *5 and represents any one of the followings: *1-S—*5-*2, *1-O—*5-*2, *1-C(—R₁₁,—R₁₂)—*5-*2, and *1-*5-CH═CH—*2, where R₁₁and R₁₂each independently represent an alkyl group, and R₁₁and R₁₂may bind together to form a ring; Y₂is a group including *3, *4 and *6 and represents any one of the followings: *4=*6-S—*3, *4=*6-O—*3, *4=*6-C(—R₅₁, —R₅₂)—*3, *4=*6-CH═CH—*3, and *4=CH—CH═*6-*3, where R₅₁and R₅₂each independently represent an alkyl group and R₅₁and R₅₂may bind together to form a ring; and a group including A to carbon atoms represented by *5 and *6 is represented by general formula (2) or (3).
(In general formula (2), R₁₃to R₁₅each independently represent a hydrogen atom, an alkyl group or an aryl group; and n represents an integer of 0 to 2, and in general formula (3), R₁₆represents a hydrogen atom, a phenyl group, a thiol group, an alkoxy group, an aryloxy group or a halogen atom; and R₁₇and R₁₈each independently represent a hydrogen atom, an alkyl group or an alkyloxycarbonyl group).

Advantageous Effects of Invention

Owing to the compound provided by the present invention, growth suppression, cellular division suppression, metastasis suppression, functional inhibition and cytocidal action of cancer cells can be mediated even in sites where cancer cells are overlooked by surgical excision and hardly excised out. More specifically, the present invention provides a cancer cell inhibitory drug. Further, of the cancer cells, particularly against cancer stem cells, these effects are significantly exerted. Furthermore, cancer stem cells can be easily detected and the site of the cancer stem cells can be accurately determined. More specifically, the present invention provides a cancer stem-cell detection probe.

DESCRIPTION OF EMBODIMENTS

Now, embodiments of the present invention will be described below.
A cancer cell inhibitory drug of the present invention, particularly, a cancer stem-cell inhibitory drug which is selectively taken into cancer cells, particularly, into cancer stem cells, thereby inhibiting cancer stem cells, and a cancer stem-cell detection probe will be described; however, the present invention is not limited to these.

Cancer Cell Inhibitory Drug

The cancer cell inhibitory drug refers to a composition having functions of suppressing growth, cellular division, metastasis and function of cancer cells and killing cancer cells. Furthermore, cancer cells can be detected and observed by measuring luminescence of the compound of the present invention.
A compound according to the present invention contains a compound represented by general formula (1).
Regarding compound represented by general formula (1)
In general formula (1), R₁and R₂each independently represent an alkyl group, a carboxylalkyl group, an alkoxycarbonylalkyl group or an alkylcarbonyloxyalkyl group; R₃to R₁₀each independently represent a hydrogen atom, an alkyl group, an aryl group, an alkoxy group, a halogen atom, an alkoxysulfonyl group, a N-alkylsulfamoyl group, an alkyloxycarbonyl group, a carbamoyl group or a N-alkylcarbamoyl group; R₃and R₄, R₅and R₆, R₇and R₈, and R₉and R₁₀may be each independently cyclized to form a benzene ring; X₁ ⁻ represents an anionic group; Y₁is a group including *1, *2 and *5, and represents any one of the followings:
*1-S—*5-*2 (exemplified in compounds 1 to 12, 29, 39 and 42),
*1-O—*5-2 (exemplified in compounds 13 to 19, 23, 28, 33, 38, 41, 44, 56 and 57),
*1-C(—R₁₁,—R₁₂)—*5-*2 (exemplified in compounds 20 to 22, 24 to 27, 30 to 32, 37, 40, 43, 49 to 55 and 58), and
*1-*5-CH═CH—*2 (exemplified in compounds 34 to 36 and 45 to 48),
where R₁₁and R₁₂each independently represent an alkyl group, R₁₁and R₁₂may bind together to form a ring;
Y₂is a group including *3, *4 and *6 and represents any one of the followings:
*4=*6-S—*3 (exemplified in compounds 1 to 12, 29, 34, 39 and 42),
*4=*6-O—*3 (exemplified in compounds 13 to 19, 23, 28, 33, 35, 38, 41, 44, 56 and 57),
*4=*6-C(—R₅₁, —R₅₂)—*3 (exemplified in compounds 20 to 22, 24 to 27, 30 to 32, 36, 37, 40, 43, 49 to 55 and 58),
*4=*6-CH═CH—*3 (exemplified in compounds 45, 47 and 48) and
*4=CH—CH═*6-*3 (exemplified in compound 46),
where R₅₁and R₅₂each independently represent an alkyl group and R₅₁and R₅₂may bind together to form a ring; and a group including A to carbon atoms represented by *5 and *6 is represented by general formula (2) or (3):
In general formula (2), R₁₃to R₁₅each independently represent a hydrogen atom, an alkyl group or an aryl group; and n represents an integer of 0 to 2, in general formula (3), R₁₆represents a hydrogen atom, a phenyl group, a thiol group, an alkoxy group, an aryloxy group or a halogen atom; and R₁₇and R₁₈each independently represent a hydrogen atom, an alkyl group or an alkyloxycarbonyl group.
In general formula (1), examples of the alkyl group represented by R₁and R₂include, but are not particularly limited to, a methyl group, an ethyl group, a propyl group, a butyl group, a pentyl group and a hexyl group.
In general formula (1), examples of the carboxylalkyl group represented by R₁and R₂include, but are not particularly limited to, a carboxylmethyl group, a carboxylethyl group and a carboxylpropyl group.
In general formula (1), examples of the alkoxycarbonylalkyl group represented by R₁and R₂include, but are not particularly limited to, a methoxycarbonylmethyl group, a methoxycarbonylethyl group, an ethoxycarbonylethyl group, a butoxycarbonylethyl group and a methoxycarbonylpropyl group; and examples of the alkylcarbonyloxyalkyl group include, but are not particularly limited to, a methylcarbonyloxymethyl group, an ethylcarbonyloxymethyl group, an ethylcarbonyloxyethyl group, an ethylcarbonyloxybutyl group and a propylcarbonyloxymethyl group.
In general formula (1), examples of the alkyl groups represented by R₃to R₁₀include, but are not particularly limited to, a methyl group, an ethyl group, a propyl group, a butyl group, a pentyl group and a hexyl group.
In general formula (1), examples of the aryl groups represented by R₃to R₁₀include, but are not particularly limited to, a phenyl group, a 2-bromophenyl group, a 3-bromophenyl group, a 4-bromophenyl group, a 2-methoxyphenyl group, a 3-methoxyphenyl group, a 4-methoxyphenyl group, a 2-thiomethylphenyl group, a 3-thiomethylphenyl group, a 4-thiomethylphenyl group and a naphthyl group.
In general formula (1), examples of the alkoxy groups represented by R₃to R₁₀include, but are not particularly limited to, a methoxy group, an ethoxy group, a propoxy group and a butoxy group.
In general formula (1), examples of the halogen atoms represented by R₃to R₁₀include a fluorine atom, a chlorine atom, a bromine atom and an iodine atom.
In general formula (1), examples of the alkoxysulfonyl groups represented by R₃to R₁₀include, but are not particularly limited to, a methoxysulfonyl group and an ethoxysulfonyl group.
In general formula (1), examples of the N-alkylsulfamoyl groups represented by R₃to R₁₀include, but are not particularly limited to, a N-methylsulfamoyl group, a N-ethylsulfamoyl group, a N,N-dimethylsulfamoyl group and a N,N-diethylsulfamoyl group.
In general formula (1), examples of the alkyloxycarbonyl groups represented by R₃to R₁₀include, but are not particularly limited to, a methyloxycarbonyl group, an ethyloxycarbonyl group, a propyloxycarbonyl group and a butyloxycarbonyl group.
In general formula (1), examples of the N-alkylcarbamoyl groups represented by R₃to R₁₀include, but are not particularly limited to, a N-methylcarbamoyl group, a N-ethylcarbamoyl group, a N,N-dimethylcarbamoyl group and a N,N-diethylcarbamoyl group.
R₃to R₁₀in general formula (1) each independently represent preferably a hydrogen atom, a halogen atom, a phenyl group or an alkoxy group, and more preferably a hydrogen atom or a phenyl group.
In general formula (1), examples of the anionic group represented by X₁ ⁻include, but are not particularly limited to, a chloride ion, a bromide ion, an iodide ion, a sulfate ion, a nitrate ion, a methanesulfonate ion, a p-toluenesulfonate ion, a tetrafluoroborate ion and a hexafluorophosphate ion.
In general formula (1), examples of the alkyl groups represented by R₁₁, R₁₂, R₅₁and R₅₂in Y₁and Y₂include, but are not particularly limited to, a methyl group, an ethyl group, a propyl group, a butyl group, a pentyl group, a hexyl group and a 2-ethylhexyl group. R₁₁and R₁₂are favorably the same; however they may differ. R₅₁and R₅₂are favorably the same; however they may differ.
In general formula (1), R₁₁and R₁₂or R₅₁and R₅₂may bind together to form an aliphatic ring such as a cyclohexane ring and a cyclopentane ring.
In general formula (2), examples of the alkyl groups represented by R₁₃to R₁₅include, but are not particularly limited to, a methyl group, an ethyl group, a propyl group, a butyl group, a pentyl group and a hexyl group.
In general formula (2), examples of the aryl groups represented by R₁₃to R₁₅include, but are not particularly limited to, a phenyl group, a 2-bromophenyl group, a 3-bromophenyl group, a 4-bromophenyl group, a 2-methoxyphenyl group, a 3-methoxyphenyl group, a 4-methoxyphenyl group, a 2-thiomethylphenyl group, a 3-thiomethylphenyl group and a 4-thiomethylphenyl group.
In general formula (3), examples of the thiol group represented by R₁₆include a methanethiol group, a butanethiol group and a benzenethiol group. Alternatively, the thiol group represented by R₁₆may be a phenylthio group.
In general formula (3), examples of the alkoxy group represented by R₁₆include a methoxy group, an ethoxy group, a propoxy group and a butoxy group.
In general formula (3), examples of the aryloxy group represented by R₁₆include a phenoxy group and a phenoxy group which may have a substituent.
In general formula (3), examples of the halogen atoms represented by R₁₆include a fluorine atom, a chlorine atom, a bromine atom and an iodine atom.
In general formula (3), examples of the alkyl groups represented by R₁₇and R₁₈include, but are not particularly limited to, a methyl group, an ethyl group, a propyl group, a butyl group, a pentyl group and a hexyl group.
In general formula (3), examples of the alkyloxycarbonyl groups represented by R₁₇and R₁₈include, but are not particularly limited to, a methyloxycarbonyl group, an ethyloxycarbonyl group, a propyloxycarbonyl group and a butyloxycarbonyl group.
Regarding compound represented by general formula (4) As a favorable compound of the present invention, a compound represented by general formula (3) can be mentioned.
In general formula (4), R₁₉and R₂₀each independently represent an alkyl group, a carboxylalkyl group, an alkylcarbonyloxyalkyl group or an alkoxycarbonylalkyl group; and R₂₁to R₂₈each independently represent a hydrogen atom, an alkyl group, an aryl group, an alkoxy group, a halogen atom, an alkoxysulfonyl group, a N-alkylsulfamoyl group, an alkyloxycarbonyl group or a N-alkylcarbamoyl group. R₂₁and R₂₂, R₂₃and R₂₄, R₂₅and R₂₆and R₂₇and R₂₈may be each independently cyclized to form a benzene ring; R₂₉to R₃₁each independently represent a hydrogen atom, an alkyl group or an aryl group; and m represents an integer of 0 to 2. X₂ ⁻represents an anionic group; and Y₃and Y₄each independently represent an oxygen atom, a sulfur atom or an alkylene group and the alkylene group may have a substituent being alkyl group. If the alkylene group has two or more substituents being alkyl groups, they may bind together to form an aliphatic ring.
In general formula (4), examples of the alkyl group represented by R₁₉and R₂₀include, but are not particularly limited to, a methyl group, an ethyl group, a propyl group, a butyl group, a pentyl group and a hexyl group.
In general formula (4), examples of the carboxylalkyl group represented by R₁₉and R₂₀include, but are not particularly limited to, a carboxylmethyl group, a carboxylethyl group and a carboxylpropyl group.
In general formula (4), examples of the alkoxycarbonylalkyl group represented by R₁₉and R₂₀include, but are not particularly limited to, a methoxycarbonylmethyl group, a methoxycarbonylethyl group, an ethoxycarbonylethyl group, a butoxycarbonylethyl group and a methoxycarbonylpropyl group.
Examples of the alkylcarbonyloxyalkyl group include, but are not particularly limited to, a methylcarbonyloxymethyl group, an ethylcarbonyloxymethyl group, an ethylcarbonyloxyethyl group, an ethylcarbonyloxybutyl group and a propylcarbonyloxymethyl group.
In general formula (4), examples of the alkyl groups represented by R₂₁to R₂₈include, but are not particularly limited to, a methyl group, an ethyl group, a propyl group, a butyl group, a pentyl group and a hexyl group.
In general formula (4), examples of the aryl groups represented by R₂₁to R₂₈include, but are not particularly limited to, a phenyl group, a 2-bromophenyl group, a 3-bromophenyl group, a 4-bromophenyl group, a 2-methoxyphenyl group, a 3-methoxyphenyl group, a 4-methoxyphenyl group, a 2-thiomethylphenyl group, a 3-thiomethylphenyl group, a 4-thiomethylphenyl group and a naphthyl group.
In general formula (4), examples of the alkoxy groups represented by R₂₁to R₂₈include, but are not particularly limited to, a methoxy group, an ethoxy group, a propoxy group and a butoxy group.
In general formula (4), examples of the halogen atoms represented by R₂₁to R₂₈include a fluorine atom, a chlorine atom, a bromine atom and an iodine atom.
In general formula (4), examples of the alkoxysulfonyl groups represented by R₂₁to R₂₈include, but are not particularly limited to, a methoxysulfonyl group and an ethoxysulfonyl group.
In general formula (4), examples of the N-alkylsulfamoyl groups represented by R₂₁to R₂₈include, but are not particularly limited to, a N-methylsulfamoyl group, a N-ethylsulfamoyl group, a N,N-dimethylsulfamoyl group and a N,N-diethylsulfamoyl group.
In general formula (4), examples of the alkyloxycarbonyl groups represented by R₂₁to R₂₈include, but are not particularly limited to, a methyloxycarbonyl group, an ethyloxycarbonyl group, a propyloxycarbonyl group and a butyloxycarbonyl group.
In general formula (4), examples of the N-alkylcarbamoyl groups represented by R₂₁to R₂₈include, but are not particularly limited to, a N-methylcarbamoyl group, a N-ethylcarbamoyl group, a N,N-dimethylcarbamoyl group and a N,N-diethylcarbamoyl group.
R₂₁to R₂₈in general formula (4) each independently represent preferably a hydrogen atom, a halogen atom, a phenyl group or an alkoxy group, and more preferably a hydrogen atom or a phenyl group.
In general formula (4), examples of the alkyl groups represented by R₂₉to R₃₁include, but are not particularly limited to, a methyl group, an ethyl group, a propyl group, a butyl group, a pentyl group and a hexyl group.
In general formula (4), examples of the aryl group represented by R₂₉to R₃₁include, but are not particularly limited to, a phenyl group, a 2-bromophenyl group, a 3-bromophenyl group, a 4-bromophenyl group, a 2-methoxyphenyl group, a 3-methoxyphenyl group, a 4-methoxyphenyl group, a 2-thiomethylphenyl group, a 3-thiomethylphenyl group and a 4-thiomethylphenyl group.
In general formula (4), examples of the anionic group represented by X₂ ⁻include, but are not particularly limited to, a chloride ion, a bromide ion, an iodide ion, a sulfate ion, a nitrate ion, a methanesulfonate ion, a p-toluenesulfonate ion, a tetrafluoroborate ion and a hexafluorophosphate ion.
In general formula (4), Y₃and Y₄each independently represent an oxygen atom, a sulfur atom or an alkylene group and the alkylene group may have a substituent being alkyl group. If the alkylene group has two or more substituents being alkyl groups, they may bind together to form an aliphatic ring.
Examples of the alkylene group herein include, but are not particularly limited to, a methylene group, an ethylene group, a propylene group, a butylene group, a pentylene group, a hexylene group and a 2-ethylhexylene group. Examples of the aliphatic ring to be formed include, but are not particularly limited to, a cyclohexane ring and a cyclopentane ring.
The compounds represented by general formula (4) in the present invention are mostly commercially available and can be purchased and also synthesized in the same manner as in known methods (for example, NPL-3).
In the above compounds (A) to (D), R₁₉to R₃₁, X₂ ⁻, Y₃, and Y₄are the same as defined in R₁₉to R₃₁, X₂ ⁻, Y₃and Y₄in compounds (A) to (D) in general formula (4). Furthermore, R in compound (A) represents an alkyl group such as a methyl group and an ethyl group.
More specifically, a compound represented by general formula (4) where m represents 0 is obtained by coupling compound (A), compound (C) and compound (D). A compound represented by general formula (4) where m represents 0 to 2 is obtained by coupling compound (B), compound (C) and compound (D).
Examples of the coupling method are not particularly limited. For example, a method of using compound (A) where m represents 0 will be described below as an embodiment.
The use amount of compound (C) in a coupling step relative to compound (A) (1 mole) is 0.1 to 1.2 times by mole, preferably 0.5 to 1.1 times by mole, and more preferably 0.8 to 1.0 times by mole.
The use amount of compound (D) in a coupling step relative to compound (A) (1 mole) is 0.1 to 2 times by mole, preferably 0.5 to 1.5 times by mole, and more preferably 0.8 to 1.2 times by mole.
The compound (C) and compound (D), which are not limited, may be the same or different; however, they are preferably the same compounds in view of process. The use amount of compound (C) and compound (D) relative to compound (A) (1 mole) when they are the same compounds, is 0.1 to 3 times by mole, preferably 0.5 to 2 times by mole, and more preferably 0.8 to 1.5 times by mole.
The coupling step can be performed in the absence of a solvent; however, it is favorably performed in the presence of a solvent. The solvent is not particularly limited as long as it is not involved in a reaction. Examples of the solvent include ester solvents such as methyl acetate, ethyl acetate, isopropyl acetate and butyl acetate; nitrile solvents such as acetonitrile, propionitrile and benzonitrile; aromatic solvents such as benzene, toluene, xylene, ethylbenzene, chlorobenzene and mesitylene; ether solvents such as diisopropyl ether, methyl-tert-butyl ether and tetrahydrofuran; alcohol solvents such as methanol, ethanol, n-propyl alcohol, iso-propyl alcohol, butyl alcohol and diethylene glycol; ketone solvents such as acetone and methylethyl ketone; dimethylformamide (DMF), dimethylsulfoxide (DMSO), water and acetic acid. Preferably, alcohol solvents such as methanol, ethanol, n-propyl alcohol, iso-propyl alcohol, butyl alcohol and diethylene glycol, water and acetic acid, and more preferably e.g., ethanol, iso-propyl alcohol and diethylene glycol and acetic acid are mentioned. Furthermore, two or more types of solvents can be used in combination and the mixing ratio of solvents used in combination can be determined at discretion.
The use amount of reaction solvent in the coupling step relative to compound (A) falls within the range of 0.1 to 1000 times by weight, preferably 0.5 to 500 times by weight, and more preferably 1.0 to 150 times by weight.
The reaction temperature in the coupling step falls within the range of −80 to 250° C., preferably −20 to 200° C., and more preferably 10 to 170° C. The reaction is generally completed within 24 hours.
In the coupling step, if an acid or a base is added as necessary, the reaction swiftly proceeds. The acid to be used is not particularly limited. Examples of the acid include inorganic acids such as hydrochloric acid, sulfuric acid and phosphoric acid; organic acids such as p-toluenesulfonic acid, formic acid, acetic acid, propionic acid, trifluoroacetic acid and acetic anhydride; strongly acidic ion exchange resins such as Amberlite (Rohm and Haas) and Amberlyst (Rohm and Haas); and inorganic acid salts such as ammonium formate and ammonium acetate. More preferably, an inorganic acid salt such as ammonium formate or ammonium acetate, and more preferably ammonium acetate is mentioned. The use amount of acid relative to compound (A) (1 mole) is 0.001 to 50 times by mole, preferably 0.01 to 10 times by mole, and more preferably 0.1 to 5 times by mole.
Specific examples of the base to be used in the coupling step include metal alkoxides such as potassium tert-butoxide, sodium tert-butoxide, sodium methoxide and sodium ethoxide; organic bases such as piperidine, pyridine, 2-methylpyridine, dimethylaminopyridine, diethylamine, triethylamine, isopropylethylamine, sodium acetate, potassium acetate, 1,8-diazabicyclo[5,4,0]undec-7-ene (hereinafter, simply referred to as DBU) and ammonium acetate; organic bases such as N-butylithium and tert-butylmagnesium chloride; and inorganic bases such as sodium borohydride, metallic sodium, sodium hydride and sodium carbonate. Preferably, potassium tert-butoxide, sodium methoxide, sodium ethoxide, piperidine, dimethylaminopyridine, sodium acetate and ammonium acetate; and more preferably sodium methoxide, piperidine, sodium acetate and ammonium acetate are mentioned. The use amount of base as mentioned above relative to compound (A) (1 mole) is 0.1 to 20 times by mole, preferably 0.5 to 8 times by mole, and more preferably 1.0 to 4 times by mole.
After completion of the reaction, a reaction product is diluted with water or precipitated with an acid such as hydrochloric acid to obtain a compound represented by general formula (4).
To the obtained compound, isolation/purification methods generally used for organic compounds can be applied. For example, a reaction solution is acidified with an acid such as hydrochloric acid to precipitate a solid substance. The solid substrate is separated by filtration, neutralized with e.g., sodium hydroxide and concentrated to obtain a crude product. The crude product is further purified by e.g., recrystallization using e.g., acetone or methanol, or a column using silica gel. The crude product can be highly purified by employing these methods alone or in combination with two or more.
Regarding compound represented by general formula (5) As a preferable compound of the present invention, a compound represented by general formula (5) can be mentioned.
In general formula (5), R₃₄and R₃₅each independently represents an alkyl group, a carboxylalkyl group or an alkoxycarbonylalkyl group; and R₃₆to R₄₃each independently represent a hydrogen atom, an alkyl group, an aryl group, an alkoxy group, a halogen atom, an alkoxysulfonyl group, a N-alkylsulfamoyl group, an alkyloxycarbonyl group or a N-alkylcarbamoyl group. R₃₆and R₃₇, R₃₈and R₃₉, R₄₀and R₄₁and R₄₂and R₄₃may be each independently cyclized to form a benzene ring. R₄₄represents a hydrogen atom, a phenyl group, a thiol group, an alkoxy group, an aryloxy group or a halogen atom; and R₄₅and R₄₆each independently represent a hydrogen atom, an alkyl group or an alkylcarbonyloxy group. X₃ ⁻represents an anionic group; and Y₅and Y₆each independently represent an oxygen atom, a sulfur atom or an alkylene group and the alkylene group may have substituents being alkyl groups which may bind together to form an aliphatic ring.
In general formula (5), examples of the alkyl group represented by R₃₄and R₃₅include, but are not particularly limited to, a methyl group, an ethyl group, a propyl group, a butyl group, a pentyl group and a hexyl group.
In general formula (5), examples of the carboxylalkyl group represented by R₃₄and R₃₅include, but are not particularly limited to, a carboxylmethyl group, a carboxylethyl group and a carboxylpropyl group.
In general formula (5), examples of the alkoxycarbonylalkyl group represented by R₃₄and R₃₅include, but are not particularly limited to, a methoxycarbonylmethyl group, a methoxycarbonylethyl group, an ethoxycarbonylethyl group, a butoxycarbonylethyl group and a methoxycarbonylpropyl group; and in general formula (5), examples of the alkyl groups represented by R₃₆to R₄₃include, but are not particularly limited to, a methyl group, an ethyl group, a propyl group, a butyl group, a pentyl group and a hexyl group.
In general formula (5), examples of the aryl groups represented by R₃₆to R₄₃include, but are not particularly limited to, a phenyl group, a 2-bromophenyl group, a 3-bromophenyl group, a 4-bromophenyl group, a 2-methoxyphenyl group, a 3-methoxyphenyl group, a 4-methoxyphenyl group, a 2-thiomethylphenyl group, a 3-thiomethylphenyl group, a 4-thiomethylphenyl group and a naphthyl group.
In general formula (5), examples of the alkoxy groups represented by R₃₆to R₄₃include, but are not particularly limited to, a methoxy group, an ethoxy group, a propoxy group and a butoxy group.
In general formula (5), examples of the halogen atoms represented by R₃₆to R₄₃include a fluorine atom, a chlorine atom, a bromine atom and an iodine atom.
In general formula (5), examples of the alkoxysulfonyl groups represented by R₃₆to R₄₃include, but are not particularly limited to, a methyl sulfonate group and an ethyl sulfonate group.
In general formula (5), examples of the alkylsulfamoyl groups represented by R₃₆to R₄₃include, but are not particularly limited to, a monomethylamide sulfonate group, a monoethylamide sulfonate group, a dimethylamide sulfonate group and a diethylamide sulfonate group.
In general formula (5), examples of the alkylcarbonyloxyalkyl groups represented by R₃₆to R₄₃include, but are not particularly limited to, a methylcarbonyloxymethyl group, an ethylcarbonyloxymethyl group, an ethylcarbonyloxyethyl group, an ethylcarbonyloxybutyl group and a propylcarbonyloxymethyl group.
In general formula (5), examples of the N-alkylcarbamoyl groups represented by R₃₆to R₄₃include, but are not particularly limited to, a N-methylcarbamoyl group, a N-ethylcarbamoyl group, a N,N-dimethylcarbamoyl group and a N,N-diethylcarbamoyl group.
R₃₆to R₄₃in general formula (5) each independently represent preferably a hydrogen atom, a halogen atom, a phenyl group or an alkoxy group, and more preferably a hydrogen atom or a phenyl group.
In general formula (5), examples of the thiol group represented by R₄₄include a mercaptomethyl group, a mercaptobutyl group and a mercaptophenyl group. Alternatively, the thiol group represented by R₄₄may be a phenylthio group.
In general formula (5), examples of the alkoxy group represented by R₄₄include a methoxy group, an ethoxy group, a propoxy group and a butoxy group.
In general formula (5), examples of the aryloxy group represented by R₄₄include a phenoxy group and a phenoxy group which may have a substituent.
In general formula (5), examples of the halogen atom represented by R₄₄include a fluorine atom, a chlorine atom, a bromine atom and an iodine atom.
In general formula (5), examples of the alkyl group represented by R₄₅and R₄₆include, but are not particularly limited to, a methyl group, an ethyl group, a propyl group, a butyl group, a pentyl group and a hexyl group.
In general formula (5), examples of the alkyloxycarbonyl groups represented by R₄₅and R₄₆include, but are not particularly limited to, a methyloxycarbonyl group, an ethyloxycarbonyl group, a propyloxycarbonyl group and a butyloxycarbonyl group.
In general formula (5), examples of the anionic group represented by X₃ ⁻include, but are not particularly limited to, a chloride ion, a bromide ion, an iodide ion, a sulfate ion, a nitrate ion, a methanesulfonate ion, a p-toluenesulfonate ion, a tetrafluoroborate ion and a hexafluorophosphate ion.
In general formula (5), examples of the alkylene groups represented by Y₅and Y₆include, but are not particularly limited to, a methylene group, an ethylene group, a propylene group, a butylene group, a pentylene group, a hexylene group and a 2-ethylenehexyl group. Examples of the alkyl group serving as a substituent of an alkylene group include, but are not particularly limited to, a methyl group, an ethyl group, a propyl group and a butyl group.
The compounds represented by general formula (5) in the present invention are mostly commercially available and can be purchased and also synthesized in the same manner as in known methods (for example, NPL-4).
In compounds (E) to (G), R₃₅to R₄₄, X₃ ⁻, Y₅, and Y₆are the same as defined in R₃₅to R₄₄, X₃ ⁻, Y₅, and Y₆in compounds (E) to (G) in general formula (5).
More specifically, a compound represented by general formula (5) can be obtained by coupling compounds (E) to (G). Examples of the coupling method are not particularly limited. For example, a method described below is mentioned as an embodiment.
The use amount of compound (F) in a coupling step relative to compound (E) (1 mole) is 0.1 to 1.2 times by mole, preferably 0.5 to 1.1 times by mole, and more preferably 0.8 to 1.0 times by mole.
The use amount of compound (G) in a coupling step relative to compound (E) (1 mole) is 0.1 to 2 times by mole, preferably 0.5 to 1.5 times by mole, and more preferably 0.8 to 1.2 times by mole.
The compound (F) and compound (G), which are not limited, may be the same or different; however, they are preferably the same compounds in view of process. The use amount of compound (F) and compound (G) relative to compound (E) (1 mole) when they are the same compounds, is 0.1 to 3 times by mole, preferably 0.5 to 2 times by mole, and more preferably 0.8 to 1.5 times by mole.
The coupling step can be performed in the absence of a solvent; however, it is favorably performed in the presence of a solvent. The solvent is not particularly limited as long as it is not involved in a reaction. Examples of the solvent include ester solvents such as methyl acetate, ethyl acetate, isopropyl acetate and butyl acetate; nitrile solvents such as acetonitrile, propionitrile and benzonitrile; aromatic solvents such as benzene, toluene, xylene, ethylbenzene, chlorobenzene and mesitylene; ether solvents such as diisopropyl ether, methyl-tert-butyl ether and tetrahydrofuran; alcohol solvents such as methanol, ethanol, n-propyl alcohol, iso-propyl alcohol, butyl alcohol and diethylene glycol; ketone solvents such as acetone and methylethyl ketone; dimethylformamide (DMF), dimethylsulfoxide (DMSO), water and acetic acid. Preferably, alcohol solvents such as methanol, ethanol, n-propyl alcohol, iso-propyl alcohol, butyl alcohol and diethylene glycol, water and acetic acid, and more preferably e.g., ethanol, iso-propyl alcohol and diethylene glycol and acetic acid are mentioned. Furthermore, two or more types of solvents can be used in combination and the mixing ratio of solvents used in combination can be determined at discretion.
The use amount of reaction solvent in the coupling step relative to compound (E) falls within the range of 0.1 to 1000 times by weight, preferably 0.5 to 500 times by weight, and more preferably 1.0 to 150 times by weight.
The reaction temperature in the coupling step falls within the range of −80 to 250° C., preferably −20 to 200° C., and more preferably 10 to 170° C. The reaction is generally completed within 24 hours.
In the coupling step, if an acid or a base is added as necessary, the reaction swiftly proceeds. The acid to be used is not particularly limited. Examples of the acid include inorganic acids such as hydrochloric acid, sulfuric acid and phosphoric acid; organic acids such as p-toluenesulfonic acid, formic acid, acetic acid, propionic acid, trifluoroacetic acid and acetic anhydride; strongly acidic ion exchange resins such as Amberlite (Rohm and Haas) and Amberlyst (Rohm and Haas); and inorganic acid salts such as ammonium formate and ammonium acetate. More preferably, an inorganic acid salt such as ammonium formate or ammonium acetate, and more preferably ammonium acetate is mentioned. The use amount of acid relative to compound (E) (1 mole) is 0.001 to 50 times by mole, preferably 0.01 to 10 times by mole, and more preferably 0.1 to 5 times by mole.
Specific examples of the base to be used in the coupling step include metal alkoxides such as potassium tert-butoxide, sodium tert-butoxide, sodium methoxide and sodium ethoxide; organic bases such as piperidine, pyridine, 2-methylpyridine, dimethylaminopyridine, diethylamine, triethylamine, isopropylethylamine, sodium acetate, potassium acetate, 1,8-diazabicyclo[5,4,0]undec-7-ene (hereinafter, simply referred to as DBU) and ammonium acetate; organic bases such as N-butylithium and tert-butylmagnesium chloride; and inorganic bases such as sodium borohydride, metallic sodium, sodium hydride and sodium carbonate. Preferably, potassium tert-butoxide, sodium methoxide, sodium ethoxide, piperidine, dimethylaminopyridine, sodium acetate and ammonium acetate; and more preferably sodium methoxide, piperidine, sodium acetate and ammonium acetate are mentioned. The use amount of base as mentioned above relative to compound (E) (1 mole) is 0.1 to 20 times by mole, preferably 0.5 to 8 times by mole, and more preferably 1.0 to 4 times by mole.
After completion of the reaction, a reaction product is diluted with water or precipitated with an acid such as hydrochloric acid to obtain a compound represented by general formula (5).
To the obtained compound, isolation/purification methods generally used for organic compounds can be applied. For example, a reaction solution is acidified with an acid such as hydrochloric acid to precipitate a solid substance. The solid substrate is separated by filtration, neutralized with e.g., sodium hydroxide and concentrated to obtain a crude product. Furthermore, the crude product is purified by e.g., recrystallization using e.g., acetone or methanol, or a column using silica gel. The crude product can be highly purified by employing these methods alone or in combination with two or more.
Regarding compound represented by general formula (6) As a preferable compound of the present invention, a compound represented by general formula (6) can be mentioned.
In general formula (6), R₄₉and R₅₀each independently represent an alkyl group, a carboxylalkyl group, an alkylcarbonyloxyalkyl group or an alkoxycarbonylalkyl group. X₄ ⁻represents an anionic group.
In general formula (6), examples of the alkyl group represented by R₄₉and R₅₀include, but are not particularly limited to, a methyl group, an ethyl group, a propyl group, a butyl group, a pentyl group and a hexyl group.
In general formula (6), examples of the carboxylalkyl group represented by R₄₉and R₅₀include, but are not particularly limited to, a carboxylmethyl group, a carboxylethyl group and a carboxylpropyl group.
In general formula (6), examples of the alkoxycarbonylalkyl group represented by R₄₉and R₅₀include, but are not particularly limited to, a methoxycarbonylmethyl group, a methoxycarbonylethyl group, an ethoxycarbonylethyl group, a butoxycarbonylethyl group and a methoxycarbonylpropyl group; and examples of the alkylcarbonyloxyalkyl group include, but are not particularly limited to, a methylcarbonyloxymethyl group, an ethylcarbonyloxymethyl group, an ethylcarbonyloxyethyl group, an ethylcarbonyloxybutyl group and a propylcarbonyloxymethyl group.
In general formula (6), examples of the anionic group represented by X₄ ⁻include, but are not particularly limited to, a chloride ion, a bromide ion, an iodide ion, a sulfate ion, a nitrate ion and a methanesulfonate ion.
The compounds represented by general formula (6) in the present invention are mostly commercially available and can be purchased and also synthesized in the same manner as in known methods (for example, NPL-5).
In compounds (H) to (J), R₄₉, R₅₀and X₄ ⁻are the same as defined in R₄₉, R₅₀and X₄ ⁻in compounds (H) to (J) in general formula (6).
More specifically, a compound represented by general formula (6) can be obtained by coupling compounds (H) to (J). Examples of the coupling method is not particularly limited. For example, a method described below is mentioned as an embodiment.
The use amount of compound (I) in a coupling step relative to compound (H) (1 mole) is 0.1 to 1.2 times by mole, preferably 0.5 to 1.1 times by mole, and more preferably 0.8 to 1.0 times by mole.
The use amount of compound (J) in a coupling step relative to compound (H) (1 mole) is 0.1 to 2 times by mole, preferably 0.5 to 1.5 times by mole, and more preferably 0.8 to 1.2 times by mole.
The compound (I) and compound (J), which are not limited, may be the same or different; however, preferably the same compounds in view of process. The use amount of compound (I) and compound (J) relative to compound (H) (1 mole) when they are the same compounds, is 0.1 to 3 times by mole, preferably 0.5 to 2 times by mole, and more preferably 0.8 to 1.5 times by mole.
The coupling step can be performed in the absence of a solvent; however, it is favorably performed in the presence of a solvent. The solvent is not particularly limited as long as it is not involved in a reaction. Examples of the solvent include ester solvents such as methyl acetate, ethyl acetate, isopropyl acetate and butyl acetate; nitrile solvents such as acetonitrile, propionitrile and benzonitrile; aromatic solvents such as benzene, toluene, xylene, ethylbenzene, chlorobenzene and mesitylene; ether solvents such as diisopropyl ether, methyl-tert-butyl ether and tetrahydrofuran; alcohol solvents such as methanol, ethanol, n-propyl alcohol, iso-propyl alcohol, butyl alcohol and diethylene glycol; ketone solvents such as acetone and methylethyl ketone; dimethylformamide (DMF), dimethylsulfoxide (DMSO), water and acetic acid. Preferably, alcohol solvents such as methanol, ethanol, n-propyl alcohol, iso-propyl alcohol, butyl alcohol and diethylene glycol, water and acetic acid, and more preferably e.g., ethanol, iso-propyl alcohol and diethylene glycol and acetic acid are mentioned. Furthermore, two or more types of solvents can be used in combination and the mixing ratio of solvents used in combination can be determined at discretion.
The use amount of reaction solvent in the coupling step relative to compound (H) falls within the range of 0.1 to 1000 times by weight, preferably 0.5 to 500 times by weight, and more preferably 1.0 to 150 times by weight.
The reaction temperature in the coupling step falls within the range of −80 to 250° C., preferably −20 to 200° C., and more preferably 10 to 170° C. The reaction is generally completed within 24 hours.
In the coupling step, if an acid or a base is added as necessary, the reaction swiftly proceeds. The acid to be used is not particularly limited. Examples of the acid include inorganic acids such as hydrochloric acid, sulfuric acid and phosphoric acid; organic acids such as p-toluenesulfonic acid, formic acid, acetic acid, propionic acid, trifluoroacetic acid and acetic anhydride; strongly acidic ion exchange resins such as Amberlite (Rohm and Haas) and Amberlyst (Rohm and Haas); and inorganic acid salts such as ammonium formate and ammonium acetate. More preferably, an inorganic acid salt such as ammonium formate or ammonium acetate, and more preferably ammonium acetate is mentioned. The use amount of acid relative to compound (H) (1 mole) is 0.001 to 50 times by mole, preferably 0.01 to 10 times by mole, and more preferably 0.1 to 5 times by mole.
Specific examples of the base to be used in the coupling step include metal alkoxides such as potassium tert-butoxide, sodium tert-butoxide, sodium methoxide and sodium ethoxide; organic bases such as piperidine, pyridine, 2-methylpyridine, dimethylaminopyridine, diethylamine, triethylamine, isopropylethylamine, sodium acetate, potassium acetate, 1,8-diazabicyclo[5,4,0]undec-7-ene (hereinafter, simply referred to as DBU) and ammonium acetate; organic bases such as N-butylithium and tert-butylmagnesium chloride; and inorganic bases such as sodium borohydride, metallic sodium, sodium hydride and sodium carbonate. Preferably, potassium tert-butoxide, sodium methoxide, sodium ethoxide, piperidine, dimethylaminopyridine, sodium acetate and ammonium acetate; and more preferably sodium methoxide, piperidine, sodium acetate and ammonium acetate are mentioned. The use amount of base as mentioned above relative to compound (H) (1 mole) is 0.1 to 20 times by mole, preferably 0.5 to 8 times by mole, and more preferably 1.0 to 4 times by mole.
After completion of the reaction, a reaction product is diluted with water or precipitated with an acid such as hydrochloric acid to obtain a compound represented by general formula (6).
To the obtained compound, isolation/purification methods generally used for organic compounds can be applied. For example, a reaction solution is acidified with an acid such as hydrochloric acid to precipitate a solid substance. The solid substrate is separated by filtration, neutralized with e.g., sodium hydroxide and concentrated to obtain a crude product. Furthermore, the crude product is purified by e.g., recrystallization using e.g., acetone or methanol, or a column using silica gel. The crude product can be highly purified by employing these methods alone or in combination with two or more.
Now, compounds (1) to (60) will be shown below as preferable examples of the compounds according to the present invention; however, the present invention is not limited to the following examples.
The compounds of the present invention are favorably emitted by light upon irradiation with excitation light of 350 to 800 nm in wavelength.
The compounds of the present invention is characterized in that growth suppression, cellular division suppression, metastasis suppression, functional inhibition and cytocidal action of cancer cells are mediated by taking a compound selectively into the cancer cells. Further, cancer cells can be detected and observed by measuring luminescence of the compound of the present invention.
The compounds of the present invention may be used alone or in combination with two types or more for cancer inhibition, and may be used in combination with a known anti-cancer drug(s).
In the present invention, an effect is selectively exerted particularly on cancer stem cells among cancer cells.

Cancer Stem Cells

In the specification, the cancer stem cells refer to cancer cells having properties of the stem cells. The stem cells refer to cells having two functions, i.e., self-replication ability and pluripotency (ability to differentiate into various types of cells).

Applicable Cancer

Examples of the cancer to be inhibited by the compound of the present invention include, but are not particularly limited to Examples of the cancers include breast cancer, brain tumor, stomach cancer, prostatic cancer, pancreatic cancer, lung cancer, large bowel cancer, small intestine cancer, colon cancer, rectal cancer, esophagus cancer, duodenal cancer, tongue cancer, pharyngeal cancer, liver cancer, endometrium cancer, uterine cervix cancer, renal cancer, bile duct cancer, ovarian cancer, bladder cancer, skin cancer, blood vessel cancer, salivary gland cancer, thyroid cancer, parathyroid gland cancer, nasal cavity cancer, paranasal sinus cancer, penile cancer, infant solid cancer, malignant lymphoma, malignant melanoma, retina sarcoma, testicular tumor, myeloma, sarcoma, blood vessel fibroma and leukemia. Preferably, e.g., pancreatic cancer, prostatic cancer and leukemia are mentioned. Particularly, applicable cancers may include cancer stem cells or cells originated from cancer stem cells.

Test Subject

Examples of the subject used in a test for checking whether a compound of the present invention suppresses a cancer or not include, but are not particularly limited to, vertebral animals including bony fish such as Takifugu (Japanese pufferfish), Takifugu niphobles, green spotted pufferfish (Tetraodon nigroviridis), killifish and zebra fish, amphibians such as Xenopus, birds such as fowl and quail, and mammalians such as human, monkey, chimpanzee, calf, horse, pig, dog, cat, mouse, rat, guinea pig, hamster and rabbit; small animals such as rat, mouse and hamster; and large animals such as goat, pig, dog, cat, calf and horse, monkey, chimpanzee and human. Favorably, e.g., human, mouse, rat, dog and cat are mentioned.
When a compound of the present invention is used as a medicinal drug, various types of dosage forms can be selected depending upon the administration route. Examples of dosage forms that can be used include liquid, syrup, fine granule, granule, tablet, capsule, pasting medicine and drug delivery system (DDS) such as liposome.
The administration method of a compound of the present invention is not limited and oral or parenteral administration may be used. Examples of the administration method that can be used include exposure to a living body (e.g., liquid); administration such as oral, intravascular (through e.g., a vein or an artery), peroral, sublingual, intrarectal, intraperitoneal, dermal, subcutaneous, intracutaneous, intravesical, tracheal (via bronchia), intraocular and intranasal administrations; and injection, spray and application into ear or the like.
A compound of the present invention, if necessary, may contain pharmacologically or pharmaceutically acceptable additives such as a moisturizer, a surface tension moderator, a thickener, a pH moderator, a pH buffer, a preservative, an antibacterial agent, a sweetening agent, a flavor, a solubilizer, a solubilizing agent, a coating agent and a binder.
The dose of the compound of the present invention is appropriately determined depending upon a purpose for therapy or prophylaxis, and conditions such as sexuality, age, weight of a test subject, an administration route, and degree of a disease.

Transplant Model Animal

Generally, it is difficult to monitor behavior of metastatic cancer by culturing cells. Thus, in the present invention, in order to monitor behavior of metastatic cancer, particularly, a transplant model animal can be used.
Examples of the cancer-cell transplant model animal applicable to the present invention include, but are not particularly limited to, vertebral animals including bony fish such as Takifugu (Japanese pufferfish), Takifugu niphobles, green spotted pufferfish (Tetraodon nigroviridis), killifish and zebra fish, amphibians such as Xenopus, birds such as fowl and quail, mammalians such as human, monkey, chimpanzee, calf, horse, pig, dog, cat, mouse, rat, guinea pig, hamster and rabbit, and birds such as fowl and quail; small animals such as rat, mouse and hamster; and large animals such as goat, pig, dog, cat, calf and horse, monkey and chimpanzee. Favorably, e.g., mouse, rat, dog and cat are mentioned.
Of these, e.g., immunodeficiency mice and rats, are often generally used in an initial study. In this case, it is necessary to maintain an environment by use of e.g., a clean room in the period (usually, at least 3 to 6 months) during which the study is carried out. In addition, extraordinary labor cost for management during this period is required.
For the reason, among these biological samples, zebra fish is particularly preferably used in view of cost and speed (usually at least a week). Zebra fish has been recently and already recognized as a third model animal which comes next to mice and rats in the United States and the United Kingdom. It has been elucidated that, the entire genomic sequence of zebra fish has a 80% homology to that of a human and the number of genes of zebra fish is virtually the same as that of a human. Furthermore, development and structure of major organs/tissues are mutually quite resembled. Since a process from differentiation of a fertilized egg to formation of each part (organ such as heart, liver, kidney and digestive tube) can be observed through a transparent body, it is particularly preferable to use zebra fish (the inside of which can be observed non-invasively) for screening as a model animal.
Furthermore, zebra fish lay about 200 or more fertilized eggs per time. Since zebra fish having the same genetic background are obtained, zebra fish is advantageous for screening.
The method for administering a compound of the present invention is not particularly limited; however, a cancer cell inhibitory drug may be suspended in the form of a complex with an appropriate surfactant or in the form of an emulsion in breeding water. Alternatively, the cancer cell inhibitory drug may be mixed in feed or food and orally or parenterally (e.g., injection) administered.

Cancer Stem Cell Detection Probe

Since the compound of the present invention can be used for selective detection of cancer stem cells, it can be suitably used as a cancer stem-cell detection probe. More specifically, the present invention encompasses a cancer cell detection probe.
The ratio of the compound of the present invention particularly taken into cancer stem cells among the cancer cells is large. Thus, cancer stem cells can be selectively detected. Detection and confirmation of behavior of cancer stem cells by the present invention can be carried out all in vitro, ex vivo or in vivo.
A method for detecting, by use of a compound of the present invention, which is not particularly limited as long as it has no effect upon cancer stem cells, is a method for capturing state and change of a biological sample as an image. For example, visible light, near infrared light or infrared light is applied to cancer stem cells and an image is visually observed by e.g., a camera or CCD, namely, visible light observation, near infrared light observation and infrared light observation are mentioned. Alternatively, observation by a laser microscope; fluorescence observation in which excitation light is applied to a biological sample from an excitation-light source and fluorescence emitted from the biological sample is observed by a fluorescent endoscope or the like; observation by a fluorescent microscope; observation by a fluorescent endoscope; observation by a confocal fluorescence microscope; or observation by a multiphoton excitation fluorescence microscope is mentioned. Alternatively, narrow-band light observation; colight interference tomogram observation (OCT) or observation by a soft X ray microscope is mentioned. Particularly, fluorescence observation is favorable.
The wavelength of light for exciting a compound of the present invention varies depending upon the compound represented by general formula (1) and the wavelength of the excitation light is not particularly limited as long as a cancer cell detection probe of the present invention efficiently emits fluorescent light.
The wavelength is preferably, 200 to 1010 nm, more preferably 400 to 900 nm, and more preferably 480 to 800 nm. When light within a near infrared region is used, the wavelength that is used is preferably 600 to 1000 nm, and more preferably 680 to 900 nm, which is also excellent in permeability through a living body.
The source of excitation light for exciting a compound of the present invention is not particularly limited and various types of laser light sources can be used. Examples of these laser light sources include a dye laser light source, a semiconductor laser light source, an ion laser light source, a fiber laser light source, a halogen lamp, a xenon lamp and a tungsten lamp. Alternatively, if various types of optical filters are used, a favorable excitation wavelength can be obtained and fluorescence alone can be detected.
As described above, in the state where a compound of the present invention present within cancer stem cells is allowed to emit light by applying excitation light to an individual biological organism, if the cancer stem cells can be photographed, a luminescent site can be easily detected. Furthermore, if an image in light field, which is obtained by applying visible light, is combined with a fluorescent image, which is obtained by applying excitation light, with the help of an image processing unit, cancer stem cells can be more specifically observed. Furthermore, if a confocal microscope is used, a sectional optical image can be favorably obtained. Furthermore, a multiphoton excitation fluorescence microscope, since it is highly permeable to a deep portion and a spatial resolution, is favorably used for observing inside a tissue.

EXAMPLES

Now, the present invention will be more specifically described below by way of Examples. These are specific Examples for further deep understanding of the present invention and should not be construed as limiting the invention.

Example 1

Production Examples of the compounds of the present invention will be shown.
Production of Compound (1)
Under a nitrogen atmosphere, to a solution of compound (A1) (0.61 g (2.0 mmol)) in anhydrous acetic acid (10 mL), a compound (B1) (0.20 g (1.0 mmol)) and anhydrous sodium acetate (0.16 g (2.0 mmol)) were added and stirred at 100° C. for one hour. After completion of the reaction, while the reaction solution was cooled, saturated saline (100 mL) was gently added dropwise to cool the reaction solution to room temperature. Furthermore, the reaction solution was extracted twice with dichloromethane (50 mL) and dried over anhydrous sodium acetate. Thereafter, the organic layer was concentrated under reduced pressure. The residue was purified by silica gel chromatography and the purified product was recrystallized from diethyl ether to obtain the compound (1) (0.29 g (yield 59%)). The desired product was confirmed by ¹H nuclear magnetic resonance spectroscopic analysis (ECA-400, manufactured by JEOL Ltd.) and LC/TOF MS (LC/MSD TOF, manufactured by Agilent Technologies).

Production of Compound (40)

Under a nitrogen atmosphere, to a solution of compound (A2) (0.48 g (2.2 mmol)) in anhydrous acetic acid (10 mL), a compound (B2) (0.32 g (1.0 mmol)) and anhydrous sodium acetate (0.25 g (3.0 mmol)) were added and stirred at 100° C. for one hour. After completion of the reaction, while the reaction solution was cooled, saturated saline (100 mL) was gently added dropwise to cool the reaction solution to room temperature. Furthermore, the reaction solution was extracted twice with dichloromethane (50 mL) and dried over anhydrous sodium acetate. Thereafter, the organic layer was concentrated under reduced pressure. The residue was purified by silica gel chromatography and the purified product was recrystallized from diethyl ether to obtain the compound (40) (0.38 g (yield 54%)). The desired product was confirmed by ¹H nuclear magnetic resonance spectroscopic analysis (ECA-400, manufactured by JEOL Ltd.) and LC/TOF MS (LC/MSD TOF, manufactured by Agilent Technologies).

Production of Compound (46)

Under a nitrogen atmosphere, to a solution of compound (A3) (0.90 g (3.0 mmol)) in anhydrous acetic acid (15 mL), a compound (B3) (0.29 g (1.5 mmol)) and anhydrous sodium acetate (0.29 g (3.5 mmol)) were added and stirred at 100° C. for 1.5 hours. After completion of the reaction, while the reaction solution was cooled, saturated saline (100 mL) was gently added dropwise to cool the reaction solution to room temperature. Furthermore, the reaction solution was extracted twice with dichloromethane (50 mL) and dried over anhydrous sodium acetate. Thereafter, the organic layer was concentrated under reduced pressure. The residue was purified by silica gel chromatography and the purified product was recrystallized from diethyl ether to obtain the compound (46) (0.46 g (yield 64%)). The desired product was confirmed by ¹H nuclear magnetic resonance spectroscopic analysis (ECA-400, manufactured by JEOL Ltd.) and LC/TOF MS (LC/MSD TOF, manufactured by Agilent Technologies).
Furthermore, commercially available products were purchased or 32 types of compounds shown in Table 1 were obtained by a production method according to any one of the aforementioned Production Examples. The structures of these compounds were confirmed by an analyzer in the same manner as mentioned above.

Example 2

Measurement of Fluorescent Property of Compound

A 5 μM DMSO solution of each of the compounds shown in the following Table 1 was prepared. The excitation wavelength and fluorescence wavelength of the compound were measured by a FL4500 spectrofluorometric measuring machine manufactured by Hitachi High-Technologies Corporation.

TABLE 1

	Excitation	Fluorescence
Compound	wavelengthλex	wavelength λem

Compound 1	485	575
Compound 4	563	569
Compound 5	560	628
Compound 7	344	381
Compound 10	586	615
Compound 11	354	469
Compound 14	491	510
Compound 16	474	509
Compound 17	492	511
Compound 18	492	510
Compound 20	516	602
Compound 21	473	564
Compound 22	553	570
Compound 24	496	569
Compound 26	684	710
Compound 27	650	675
Compound 28	589	614
Compound 30	643	662
Compound 34	638	661
Compound 35	571	620
Compound 37	650	770
Compound 40	819	825
Compound 43	797	816
Compound 45	665	681
Compound 46	679	715
Compound 47	615	637
Compound 49	830	831
Compound 50	831	833
Compound 54	774	800
Compound 55	688	715
Compound 59	804	520
Compound 60	670	696

Example 3

Confirmation on Cancer Cell Inhibitory (Growth Suppressive) Action Against Pancreatic Cancer Cells

Experimental Example 1

Human pancreas cancer cells, KLM-1, were pre-cultured in RPMI1640 medium containing 10% FBS at 37° C. in a 5% CO₂ambient. Thereafter, 4,000 cells were seeded per well of a 96-well plate and further cultured for 24 hours. Subsequently, Compound (1) was added to the medium so as to obtain a final concentration of 10 μg/mL and cultured at 37° C. for 24 hours in a 5% CO₂ambient. The cultured cells were analyzed for viable cell count according to CellTiter-Glo Luminescent Cell Viability Assay (manufactured by Promega KK.). As a reference, the number of cells cultured in a medium containing a 0.1% dimethylsulfoxide solution (hereinafter, simply referred to as DMSO) in place of a medium containing Compound (1), in the aforementioned operation, was regarded as 100.

Experimental Examples 2 to 23

Viable cell count was analyzed in the same manner as in Experimental Example 1 except that Compound (1) of Experimental Example 1 was changed to another compounds shown in Table 2.

Comparative Examples 1 to 4

Viable cell count was analyzed in the same manner as in Experimental Example 1 except that Compound (1) was changed to comparative compounds 1 to 4.
Viable cell counts of Experimental Examples 1 to 23 and Comparative Examples 1 to 4 were analyzed to obtain growth rates. The results are shown in Table 2. Evaluation of cancer cell inhibition against the pancreatic cancer cells (KLM-1) (growth suppression) was made based on the following criteria. Note that the growth rate used in Examples refers to the rate (expressed by percentage) of viable cell count after culture relative to the number of cells at the initiation of cell culture.
A: Cancer cell growth rate is less than 20% (cancer cell inhibitory (growth suppressive) effect is extremely high)
B: Cancer cell growth rate is 20% or more and less than 50% (cancer cell inhibitory (growth suppressive) effect is high)
C: Cancer cell growth rate is 50% or more (cancer cell inhibitory (growth suppressive) effect is low)
Table 2

TABLE 2

		Cancer cell
	Compound	growth rate (%)	Evaluation

Experimental Example 1	Compound 1	31.9	B
Experimental Example 2	Compound 4	3.1	A
Experimental Example 3	Compound 5	6.4	A
Experimental Example 4	Compound 7	13.0	A
Experimental Example 5	Compound 10	13.5	A
Experimental Example 6	Compound 11	5.0	A
Experimental Example 7	Compound 16	2.0	A
Experimental Example 8	Compound 20	1.2	A
Experimental Example 9	Compound 21	38.2	B
Experimental Example 10	Compound 24	6.5	A
Experimental Example 11	Compound 26	8.6	A
Experimental Example 12	Compound 27	6.0	A
Experimental Example 13	Compound 30	18.8	A
Experimental Example 14	Compound 31	30.5	B
Experimental Example 15	Compound 34	26.6	B
Experimental Example 16	Compound 35	29.7	B
Experimental Example 17	Compound 37	4.8	A
Experimental Example 18	Compound 40	14.1	A
Experimental Example 19	Compound 43	35.6	B
Experimental Example 20	Compound 45	28.5	B
Experimental Example 21	Compound 46	16.7	A
Experimental Example 22	Compound 47	8.6	A
Experimental Example 23	Compound 49	12.0	A
Comparative Example 1	Comparative	87.0	C
	compound 1
Comparative Example 2	Comparative	86.0	C
	compound 2
Comparative Example 3	Comparative	93.0	C
	compound 3
Comparative Example 4	Comparative	75.0	C
	compound 4

As is apparent from Table 2, the compounds of the present invention have a high cancer cell inhibitory (growth suppressive) effect against the pancreatic cancer cells (KLM-1), compared to the comparative compounds.

Example 4

Observation on Cancer Cell Inhibitory (Growth Suppressive) Action Against Prostatic Cancer Cells

Experimental Example 24

Prostatic cancer cells, PC-3, were pre-cultured in RPMI1640 medium containing 10% FBS at 37° C. in a 5% CO₂ambient. Thereafter, 4,000 cells were seeded per well of a 96-well plate and further cultured for 24 hours. Subsequently, Compound (1) was added to the medium so as to obtain a final concentration of 10 μg/mL and cultured at 37° C. for 24 hours in a 5% CO₂ambient. The cultured cells were analyzed for viable cell count according to Cell Titer-Glo Luminescent Cell Viability Assay (manufactured by Promega KK.). As a reference, the number of cells cultured in a medium containing a 0.1% dimethylsulfoxide solution (hereinafter, simply referred to as DMSO) in place of a medium containing Compound (1), in the aforementioned operation, was used as 100.

Experimental Examples 25 to 36

Viable cell count was analyzed in the same manner as in Experimental Example 24 except that Compound (1) of Experimental Example 24 was changed to each compound shown in Table 2.

Comparative Examples 5 to 8

Viable cell count was analyzed in the same manner as in Experimental Example 24 except that Compound (1) of Experimental Example 24 was changed to each of Comparative compounds 1 to 4, to obtain growth rates.
Note that the growth rate used in Examples refers to the rate (expressed by percentage) of viable cell count after culture relative to the number of cells at the initiation of cell culture. The results are shown in Table 3. Evaluation criteria are the same as in the above experiments. A cancer cell growth rate exceeding 100% indicates that cells are grown.
Cancer cell inhibition (growth suppression) against prostatic cancer cells (PC-3) was evaluated based on the following criteria.
A: Cancer cell growth rate is less than 20% (cancer cell inhibitory (growth suppressive) effect is extremely high)
B: Cancer cell growth rate is 20% or more and less than 50% (cancer cell inhibitory (growth suppressive) effect is high)
C: Cancer cell growth rate is 50% or more (cancer cell inhibitory (growth suppressive) effect is low)
Table 3

TABLE 3

		Cancer cell
	Compound	growth rate (%)	Evaluation

Experimental Example 24	Compound 1	13.8	A
Experimental Example 25	Compound 4	19.5	A
Experimental Example 26	Compound 5	15.0	A
Experimental Example 27	Compound 7	15.3	A
Experimental Example 28	Compound 11	4.3	A
Experimental Example 29	Compound 16	8.4	A
Experimental Example 30	Compound 20	13.1	A
Experimental Example 31	Compound 24	10.0	A
Experimental Example 32	Compound 27	7.7	A
Experimental Example 33	Compound 43	12.3	A
Experimental Example 34	Compound 46	17.7	A
Experimental Example 35	Compound 47	13.8	A
Experimental Example 36	Compound 49	5.3	A
Comparative Example 5	Comparative	105	C
	compound1
Comparative Example 6	Comparative	68.0	C
	compound2
Comparative Example 7	Comparative	101	C
	compound3
Comparative Example 8	Comparative	88.3	C
	compound4

As is apparent from Table 3, the compounds of the present invention have a high cancer cell inhibitory (growth suppressive) effect against the prostatic cancer cells (PC-3), compared to comparative compounds.

Example 5

Observation on Cancer Stem-Cell Selective Inhibitory Action Against Chronic Myelocytic Leukemia Cells

Experimental Examples 37

Human chronic myelocytic leukemia cells, K562, were pre-cultured in RPMI1640 medium containing 10% FBS at 37° C. in a 5% CO₂ambient. Then, a fraction containing 80% or more of cancer stem cells was extracted by use of a cancer stem cell marker, ALDEFLUOR reagent (manufactured by VERITAS Corporation) and FACSAria flow cytometry (manufactured by Nippon Becton, Dickinson and Company). Subsequently, Compound (16) was added to the medium so as to obtain a final concentration of 0.05 μg/mL and cultured at 37° C. for 24 hours in a 5% CO₂ambient. The cultured cells were analyzed for viable cell count according to CellTiter-Glo Luminescent Cell Viability Assay (manufactured by Promega KK.). As a reference, the number of cells cultured in a medium containing a 0.1% dimethylsulfoxide solution (hereinafter, simply referred to as DMSO) in place of a medium containing Compound (1), in the aforementioned operation, was used as 0.1. Note that hereinafter, an ALDEFLUOR reagent positive fraction (deemed as cancer stem cells) is represented by ALDH (+), whereas an ALDEFLUOR reagent negative fraction (not deemed as cancer stem cells) is represented by ALDH (−), in some cases.

Experimental Examples 38 to 70

The same operation as in Experimental Example 37 was repeated except that Compound (16) in Experimental Example 37 was changed to the other compounds and final concentrations shown in Table 3 were used and viable cell counts were separately analyzed.

Comparative Examples 9 to 16

The same operation as in Experimental Example 37 was repeated except that Compound (16) in Experimental Example 37 was changed to Imatinib (manufactured by NOVARTIS), which is a general anticancer drug, and comparative compounds shown in Table 3 and final concentrations shown in Table 3 were used and viable cell counts were separately analyzed.
The results of Experimental Examples 37 to 70 and Comparative Examples 9 to 16 are collectively shown in Table 4. Further, the growth suppressive effect of cancer stem cells was evaluated based on the following criteria. Note that the growth rate in Examples is a value obtained by dividing viable cell count after culture by the number of cells at the initiation time of culture.
A: The growth rate of ALDH (+) is less than 0.5
(growth suppressive effect against cancer stem cells is extremely high)
B: The growth rate of ALDH (+) is 0.5 or more and less than 0.95
(growth suppressive effect against cancer stem cells is high)
C: The growth rate of ALDH (+) is 0.95 or more
(no growth suppressive effect against cancer stem cells)
Furthermore, superiority evaluation of cancer stem cells was evaluated by comparing cancer stem cells to cancer cells based on the following criteria.
A: The value of the growth rate of ALDH (+)/the growth rate of ALDH (−) is less than 0.8
(selective inhibitory effect against cancer stem cells is extremely high)
B: The value of the growth rate of ALDH (+)/the growth rate of ALDH (−) is 0.8 or more and less than 0.95
(selective inhibitory effect against cancer stem cells is high)
C: The value of the growth rate of ALDH (+)/the growth rate of ALDH (−) is 0.95 or more
(no selective inhibitory effect against cancer stem cells)

TABLE 4

	Growth	ALDH(+)
	suppression	Superiority

	Compound	Amount of dye	ALDH(+)	evaluated	ALDH(−)	ALDH(+)/ALDH(−)	evaluation

Experimental Example 37	35	0.05	μ g/mL	0.67	B	0.87	0.77	A
Experimental Example 38	35	0.5	μ g/mL	0.43	A	0.66	0.66	A
Experimental Example 39	35	1	μ g/mL	0.44	A	0.49	0.90	B
Experimental Example 40	16	0.05	μ g/mL	0.68	B	0.78	0.87	B
Experimental Example 41	16	0.5	μ g/mL	0.36	A	0.52	0.70	A
Experimental Example 42	16	1	μ g/mL	0.16	A	0.34	0.47	A
Experimental Example 43	24	0.05	μ g/mL	0.61	B	0.75	0.81	B
Experimental Example 44	24	0.5	μ g/mL	0.39	A	0.63	0.61	A
Experimental Example 45	24	1	μ g/mL	0.19	A	0.39	0.48	A
Experimental Example 46	27	10	μ g/ml	0.07	A	0.13	0.56	A
Experimental Example 47	32	10	μ g/ml	0.09	A	0.29	0.32	A
Experimental Example 48	21	10	μ g/ml	0.27	A	0.41	0.66	A
Experimental Example 49	1	10	μ g/ml	0.45	A	0.52	0.87	B
Experimental Example 50	34	10	μ g/ml	0.48	A	0.67	0.72	A
Experimental Example 51	35	10	μ g/ml	0.55	B	0.80	0.69	A
Experimental Example 52	5	10	μ g/ml	0.71	B	0.90	0.79	A
Experimental Example 53	33	10	μ g/ml	0.83	B	1.00	0.84	B
Experimental Example 54	56	10	μ g/ml	0.42	A	0.65	0.65	A
Experimental Example 55	57	10	μ g/ml	0.55	B	0.68	0.81	B
Experimental Example 56	58	10	μ g/ml	0.70	B	0.85	0.82	B
Experimental Example 57	14	10	μ g/ml	0.38	A	0.49	0.77	A
Experimental Example 58	17	10	μ g/ml	0.04	A	0.13	0.30	A
Experimental Example 59	17	1	μ g/mL	0.69	B	0.79	0.87	B
Experimental Example 60	18	10	μ g/ml	0.04	A	0.13	0.29	A
Experimental Example 61	22	10	μ g/ml	0.22	A	0.29	0.75	A
Experimental Example 62	28	10	μ g/ml	0.30	A	0.43	0.69	A
Experimental Example 63	28	1	μ g/mL	0.65	B	0.77	0.83	B
Experimental Example 64	54	10	μ g/ml	0.07	A	0.13	0.50	A
Experimental Example 65	55	10	μ g/ml	0.04	A	0.11	0.33	A
Experimental Example 66	59	10	μ g/ml	0.02	A	0.10	0.22	A
Experimental Example 67	59	1	μ g/mL	0.14	A	0.21	0.67	A
Experimental Example 68	60	10	μ g/ml	0.04	A	0.12	0.34	A
Experimental Example 69	50	10	μ g/ml	0.05	A	0.12	0.42	A
Experimental Example 70	51	10	μ g/ml	0.03	A	0.11	0.27	A
Comparative Example 9	Imatinib	0.12	μ g/ml	0.74	B	0.51	1.47	C
Comparative Example 10	Imatinib	0.24	μ g/ml	0.59	B	0.40	1.48	C
Comparative Example 11	Imatinib	0.35	μ g/ml	0.48	A	0.32	1.51	C
Comparative Example 12	Imatinib	0.47	μ g/ml	0.36	A	0.22	1.63	C
Comparative Example 13	Imatinib	0.59	μ g/ml	0.31	A	0.11	2.80	C
Comparative Example 14	Comparative compound 1	1	μ g/mL	1.00	C	0.94	1.06	C
Comparative Example 15	Comparative compound 2	1	μ g/mL	0.99	C	1.21	0.82	C
Comparative Example 16	Comparative compound 3	1	μ g/mL	1.09	C	1.38	0.79	C

Note that, in the cases of Comparative Examples 14 to 16, ALDH (+) is close to almost 1. This indicates that no suppressive effect is obtained. In contrast, a numerical value of 1 or more as an ALDH (−) value indicates that the number of cancer cells increases.
As is apparent from Table 4, it is confirmed that the compounds of the present invention has a selective inhibitory effect against cancer stem cells. More specifically, when a general anticancer agent, Imatinib, was used, an inhibitory effect against general cancer cells was observed; however, no inhibitory effect was confirmed when comparative compounds were used.

Example 6

Confirmation of Cancer Stem-Cell Selective Staining to Chronic Myelocytic Leukemia Cells

Experimental Examples 71 to 73

The cells cultured for 24 hours in each of Experimental Examples 39, 42 and 45 were subjected to nuclear staining with Hoechest33342 (manufactured by Dojindo Laboratories) and a fluorescent image observed under AXIOVERT200M inverted fluorescent microscope (manufactured by Carl Zeiss) was photographed. The ratio of ALDH (+) cells stained and the ratio (percentage) of ALDH (−) cells stained in each compound are shown in Table 5.
Table 5

TABLE 5

				Selective staining
	Compound	ALDH(+)	ALDH(−)	of cancer stem cells

Example 71	35	82.5	21.4	ALDH (+) cells are
				selectively stained
Example 72	16	99.5	18.6	ALDH (+) cells are
				selectively stained
Example 73	24	81.2	28.6	ALDH (+) cells are
				selectively stained

As is apparent from Table 5, it is found that the compound of the present invention selectively stains cancer stem cells (ALDH (+)) than general cancer cells (ALDH (−)).

Example 7

Confirmation of Inhibitory Action in Cancer Stem Cells Transplanted Animal

Experimental Example 74

From cell strain K562-KOr, which is a strain of human chronic myelocytic leukemia cells having fluorescent protein Kusabira-Orange constantly expressed, a fraction (ALDH (+)) containing 80% or more of cancer stem cells was extracted by use of a cancer stem cell marker, ALDEFLUOR reagent (manufactured by VERITAS Corporation) and FACSAria flow cytometry (manufactured by Nippon Becton, Dickinson and Company). The ALDH (+) fraction and a ALDH (−) fraction of general cancer cells were transplanted separately to zebra young fish (MieKomachi lineage, 2 days after fertilization) and the fish were raised in a 32° C. environment. Furthermore, 24 hours after transplantation, Compound (16) was added to breeding water so as to obtain a final concentration of 0.5 μm and fish were raised for two days in a 32° C. environment.
Cells transplanted to the zebra young fish were observed under MZ16F fluorescent stereoscopic microscope (manufactured by Leica Microsystems) and a fluorescent image of the cells after 24 hours was photographed and then fluorescent intensity was quantified.
As a reference, the fluorescent intensity of cells, which were cultured in the same operation method as above in a medium containing a 0.1% DMSO solution in place of Compound (16), was used.

Comparative Example 17

The numerical value of fluorescent intensity was obtained from a fluorescent image taken in the same manner as in Experimental Example 74 except that Compound (16) of Experimental Example 74 was changed to Imatinib.
The inhibition rates of ALDH (+)/ALDH (−) cell transplanted to zebra young fish in Experimental Example 74 and Comparative Example 17 are shown in Table 6. The inhibition rate herein was obtained according to the expression: 100×(1−F1/F0), where the fluorescent intensity of cells when a test substance was added is represented by F1, and the fluorescent intensity of cells when a reference substance (DMSO) was added is represented by F0.

TABLE 6

	Compound	ALDH(+)	ALDH(−)	Growth suppression rate

Example 74	16	95.0	82.0	ALDH(+) is high
Comparative	Imatinib	60.0	80.0	ALDH(−) is high
Example 17

As is apparent form Table 6, it was confirmed that the size of tumor (fluorescent region) is small compared to the case where neither compound nor Imatinib was administered. Particularly, in the group in which the compound of the present invention is administered, it was confirmed that an effect of suppressing a tumor size is preferentially obtained in a cancer stem cell (ALDH (+)) transplanted model animal.

Example 8

Confirmation of Cancer Metastasis Suppressive Effect in Cancer Cell Metastatic Foci

Region within 300 to 450 μm from a Transplanted Tumor

Experimental Example 75

From cell strain K562-KOr, in which KLM1 cells have fluorescent protein Kusabira-Orange constantly expressed, a fraction (ALDH (+)) containing 80% or more of cancer stem cells was extracted with ALDEFLUOR reagent (manufactured by VERITAS Corporation) and FACSAria flow cytometry (manufactured by Nippon Becton, Dickinson and Company). The extracted KLM1-KOr cells were transplanted to zebra young fish (MieKomachi lineage, 2 days after fertilization) and the fish were raised in a 32° C. environment. Furthermore, 24 hours after transplantation, Compound (26) (745 μmol/KgBW) was administered to yolk sac.
72 hours later, cells transplanted to the zebra young fish were observed under MZ16F fluorescent stereoscopic microscope (manufactured by Leica Microsystems) and a fluorescent image of the region within 300 to 450 μm from a transplanted tumor was photographed and then fluorescent intensity was quantified.
As a reference, the fluorescent intensity of cells, which were cultured in the same operation method as above in a medium containing a 0.1% DMSO solution in place of Compound (26), was used.

Comparative Examples 18 and 19

Fluorescent images were photographed in the same manner as in Experimental Example 26 except that Imatinib and Dasatinib were respectively used in place of the compound (26) in Experimental Example 75.
The cancer cell inhibition rates of metastatic foci of cancer cells (in the region within 300 to 450 μm from a transplanted tumor) transplanted to zebra young fish in Experimental Example 75 and Comparative Examples 18 and 19 are shown in Table 6.
The inhibition rate herein was obtained according to the expression: 100×(1−F1/F0), where the fluorescent intensity of cells when a test substance was added is represented by F1, and the fluorescent intensity of cells when a reference substance (DMSO) was added is represented by F0.
The growth suppressive effect in metastatic foci (region within 300 to 450 μm from a transplanted tumor) of cancer stem cells was evaluated based on the following criteria.
A: Inhibition rate is 70 or more
(growth suppressive effect against metastatic foci (region within 300 to 450 μm from a transplanted tumor) of cancer stem cells is extremely high)
B: Inhibition rate is 50 or more and less than 70
(growth suppressive effect against metastatic foci (region within 300 to 450 μm from a transplanted tumor) of cancer stem cells is high)
C: Inhibition rate is less than 50
(growth suppressive effect against metastatic foci (region within 300 to 450 μm from a transplanted tumor) of cancer stem cells is low)

TABLE 7

		Inhibition
	Compound	rate	Evaluation

Example 75	26	80	A
Comparative	Imatinib	60	B
Example 18
Comparative	Dasatinib	36	C
Example 19

As is apparent from Table 7, it was confirmed that the cancer stem-cell inhibition drug of the present invention has a higher metastasis suppressive effect than known anticancer agents used as comparison.

INDUSTRIAL APPLICABILITY

The compound provided by the present invention is useful as a cancer cell inhibitory drug. Furthermore, owing to the cancer cell inhibitory drug provided by the present invention, growth suppression, cellular division suppression, metastasis suppression, functional inhibition and cytocidal action of cancer cells, particularly cancer stem cells, can be mediated. In addition, cancer stem cells can be easily detected and the site of cancer stem cells can be accurately specified. The compound of the present invention is expected to widely contribute to the medical industry.
This application claims the benefit of Japanese Patent Application No. 2012-236977, filed Oct. 26, 2012, which is hereby incorporated by reference herein in its entirety.

Claims

1. A cancer cell inhibitory drug represented by general formula (1):

where R₁and R₂each independently represent an alkyl group, a carboxylalkyl group, an alkoxycarbonylalkyl group or an alkylcarbonyloxyalkyl group; R₃to R₁₀each independently represent a hydrogen atom, an alkyl group, an aryl group, an alkoxy group, a halogen atom, an alkoxysulfonyl group, a N-alkylsulfamoyl group, an alkyloxycarbonyl group, a carbamoyl group or a N-alkylcarbamoyl group; R₃and R₄, R₅and R₆, R₇and R₈, and R₉and R₁₀may be each independently cyclized to form a benzene ring;

X₁ ⁻represents an anionic group;

Y₁is a group including *1, *2, and *5 and represents any one of:

*1-S—*5-*2, *1-O—*5-*2, *1-C(—R₁₁,—R₁₂)—*5-*2, and *1-*5-CH═CH—*2,

where R₁₁and R₁₂each independently represent an alkyl group, and R₁₁and R₁₂may bind together to form a ring;

Y₂is a group including *3, *4, and *6 and represents any one of:

*4=*6-S—*3, *4=*6-O—*3, *4=*6-C(—R₅₁,—R₅₂)—*3, *4=*6-CH═CH—*3, and *4=CH—CH═*6-*3,

where R₅₁and R₅₂each independently represent an alkyl group, and R₅₁and R₅₂may bind together to form a ring; and

a group including A to carbon atoms represented by *5 and *6 is represented by general formula (2) or (3):

where R₁₃to R₁₅each independently represent a hydrogen atom, an alkyl group or an aryl group; n represents an integer of 0 to 2;

R₁₆represents a hydrogen atom, a phenyl group, a thiol group, an alkoxy group, an aryloxy group or a halogen atom; and

R₁₇and R₁₈each independently represent a hydrogen atom, an alkyl group or an alkyloxycarbonyl group.

2. The cancer cell inhibitory drug according to claim 1, wherein the general formula (1) is represented by general formula (4):

where R₁₉and R₂₀each independently represent an alkyl group, a carboxylalkyl group, an alkylcarbonyloxyalkyl group or an alkoxycarbonylalkyl group; R₂₁to R₂₈each independently represent a hydrogen atom, an alkyl group, an aryl group, an alkoxy group, a halogen atom, an alkoxysulfonyl group, a N-alkylsulfamoyl group, an alkyloxycarbonyl group or a N-alkylcarbamoyl group; R₂₁and R₂₂, R₂₃and R₂₄, R₂₅and R₂₆, and R₂₇and R₂₈may be each independently cyclized to form a benzene ring;

R₂₉to R₃₁each independently represent a hydrogen atom, an alkyl group or an aryl group; m represents an integer of 0 to 2; X₂ ⁻represents an anionic group; and

Y₃and Y₄each independently represent an oxygen atom, a sulfur atom or an alkylene group, and the alkylene group may have a substituent being alkyl groups, wherein if the alkylene group has two or more substituents being alkyl groups they may bind together to form an aliphatic ring.

3. The cancer cell inhibitory drug according to claim 1, wherein the general formula (1) is represented by general formula (5):

where R₃₄and R₃₅each independently represent an alkyl group, a carboxylalkyl group or an alkoxycarbonylalkyl group, R₃₆to R₄₃each independently represent a hydrogen atom, an alkyl group, an aryl group, an alkoxy group, a halogen atom, an alkoxysulfonyl group, a N-alkylsulfamoyl group, an alkyloxycarbonyl group or a N-alkylcarbamoyl group; R₃₆and R₃₇, R₃₈and R₃₉, R₄₀and R₄₁, and R₄₂and R₄₃may be each independently cyclized to form a benzene ring;

R₄₄represents a hydrogen atom, a phenyl group, a thiol group, an alkoxy group, an aryloxy group or a halogen atom; R₄₅and R₄₆each independently represent a hydrogen atom, an alkyl group or an alkyloxycarbonyl group; X₃ ⁻represents an anionic group; and

Y₅and Y₆each independently represent an oxygen atom, a sulfur atom or an alkylene group, and the alkylene group may have substituents being alkyl groups which may bind together to form an aliphatic ring.

4. The cancer cell inhibitory drug according to claim 1, wherein the general formula (1) is represented by general formula (6):

where R₄₉and R₅₀each independently represent an alkyl group, a carboxylalkyl group, an alkylcarbonyloxyalkyl group or an alkoxycarbonylalkyl group; and X₄ ⁻represents anionic group.

5. The cancer cell inhibitory drug according to claim 1, wherein a compound represented by the general formula (1) is a compound having a luminescence property.

6. The cancer cell inhibitory drug according to claim 1, wherein the cancer cell is a cancer stem cell.

7. A cancer stem-cell detection probe comprising the cancer cell inhibitory drug according to claim 6 as an active ingredient.