US20140034297A1 - Dispersion of compounds for the stimulation of biogenic gas generation in deposits of carbonaceous material - Google Patents
Dispersion of compounds for the stimulation of biogenic gas generation in deposits of carbonaceous material Download PDFInfo
- Publication number
- US20140034297A1 US20140034297A1 US13/847,756 US201313847756A US2014034297A1 US 20140034297 A1 US20140034297 A1 US 20140034297A1 US 201313847756 A US201313847756 A US 201313847756A US 2014034297 A1 US2014034297 A1 US 2014034297A1
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- United States
- Prior art keywords
- carbonaceous material
- emulsion
- dispersed phase
- microorganisms
- polar
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- QURCVMIEKCOAJU-UHFFFAOYSA-N trans-isoferulic acid Natural products COC1=CC=C(C=CC(O)=O)C=C1O QURCVMIEKCOAJU-UHFFFAOYSA-N 0.000 description 1
- 229940117013 triethanolamine oleate Drugs 0.000 description 1
- DQWPFSLDHJDLRL-UHFFFAOYSA-N triethyl phosphate Chemical compound CCOP(=O)(OCC)OCC DQWPFSLDHJDLRL-UHFFFAOYSA-N 0.000 description 1
- UNXRWKVEANCORM-UHFFFAOYSA-I triphosphate(5-) Chemical compound [O-]P([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O UNXRWKVEANCORM-UHFFFAOYSA-I 0.000 description 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 description 1
- 229910052721 tungsten Inorganic materials 0.000 description 1
- 239000010937 tungsten Substances 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- MWOOGOJBHIARFG-UHFFFAOYSA-N vanillin Chemical compound COC1=CC(C=O)=CC=C1O MWOOGOJBHIARFG-UHFFFAOYSA-N 0.000 description 1
- FGQOOHJZONJGDT-UHFFFAOYSA-N vanillin Natural products COC1=CC(O)=CC(C=O)=C1 FGQOOHJZONJGDT-UHFFFAOYSA-N 0.000 description 1
- 235000012141 vanillin Nutrition 0.000 description 1
- 235000019871 vegetable fat Nutrition 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
- 238000004832 voltammetry Methods 0.000 description 1
- 239000010497 wheat germ oil Substances 0.000 description 1
- 238000012070 whole genome sequencing analysis Methods 0.000 description 1
- 239000000230 xanthan gum Substances 0.000 description 1
- 229920001285 xanthan gum Polymers 0.000 description 1
- 235000010493 xanthan gum Nutrition 0.000 description 1
- 229940082509 xanthan gum Drugs 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 description 1
- 239000002888 zwitterionic surfactant Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- E—FIXED CONSTRUCTIONS
- E21—EARTH OR ROCK DRILLING; MINING
- E21B—EARTH OR ROCK DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B43/00—Methods or apparatus for obtaining oil, gas, water, soluble or meltable materials or a slurry of minerals from wells
- E21B43/295—Gasification of minerals, e.g. for producing mixtures of combustible gases
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P3/00—Preparation of elements or inorganic compounds except carbon dioxide
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
- C12P5/023—Methane
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Definitions
- Natural gas is used as an energy source for heating, electric power generation, and transportation fuel. Natural gas is also used for the production of hydrogen, and in many manufacturing processes.
- the standard spacing of wells for natural gas production is typically forty or eighty acre spacing (i.e., 1 well/40 acres 1 well/80 acres). Due to the depths and spacing of these wells, as well as the underground distances covered by the formations, gas produced through methanogenesis may take a substantial amount of time to travel to the surface for collection. Nutrients and activation agents that have been introduced into the formation to activate or stimulate methanogenesis may be exhausted long before it has had sufficient opportunity to be widely distributed by the advective dispersion processes within the formation. This may slow, or even prevent, the enhanced conversion of carbonaceous material into natural gas, because only a small portion of the subsurface microorganism populations' growth and activity.
- Additional production of biogenic gases from carbonaceous materials found in geologic formations may be realized by supplying mixtures to microorganisms in the formations that stimulate their conversion of the carbonaceous materials to gases like hydrogen and methane.
- the mixtures may include one or more liquid dispersed phases surrounded by a liquid continuous phase that is characteristic of an emulsion.
- the mixtures may also include solid phase materials characteristic of a suspension.
- the compounds in the mixtures that stimulate biogenic gas production from the microorganisms may include activation agents and/or nutrients. These compounds may be at least partially dissolved in one or more liquid phases of the mixture that is supplied to the microorganisms.
- the materials that form a liquid phase e.g., a dispersed phase
- Exemplary mixtures may include oil-in-water and water-in-oil emulsions.
- the “oil” phase may be made of a non-polar liquid at the working temperatures of the mixture, and the “water” phase may be made from a polar liquid such as water or an aqueous solution, though other polar liquids may be used instead of (or in addition to) water.
- Exemplary mixtures may also include more complex multiple emulsions where the dispersed phase includes two or more liquid phases that are not completely miscible.
- Examples of these multiple emulsions may include water-in-oil-in-water (W/O/W) emulsions which have a dispersed phase that includes a core polar “water” droplet surrounded by a non-polar “oil” droplet surrounded by a polar “water” continuous phase.
- W/O/W water-in-oil-in-water
- Embodiments of the invention may include methods of dispersing an activation agent to a carbonaceous material to stimulate production of a biogenic gas.
- the methods may include accessing a subterranean geologic formation containing the carbonaceous material, and supplying a mixture to the formation.
- the mixture may include the activation agent mixed with a dispersed phase and a continuous phase.
- the method may also include contacting the carbonaceous material with the mixture, and distributing at least a portion of the activation agent over and/or into the carbonaceous material from the dispersed phase.
- the production of biogenic gases is increased by microorganisms that are stimulated by the distributed activation agent to convert a portion of the carbonaceous material into the biogenic gases.
- Embodiments of the invention may also include methods of providing a nutrient to a carbonaceous material to stimulate production of biogenic gas from the material.
- the methods may include accessing a geologic formation containing the carbonaceous material, and delivering a mixture to the formation that includes a dispersed phase, a continuous phase, and the nutrient incorporated into at least one of these phases.
- the mixture may contact the carbonaceous material and become available to microorganisms in proximity to the material.
- the nutrient stimulates the microorganisms to convert at least a portion of the carbonaceous material into biogenic gas, increasing the production of the biogenic gas.
- Embodiments of the invention may still further include methods of introducing multiple portions of a compound or a mixture of compounds to microorganisms in a geologic formation.
- the methods may include accessing a geologic formation containing carbonaceous material, and supplying an emulsion to the formation.
- the emulsion may have a continuous phase and a dispersed phase, and a first portion of the compound or a single component of a mixture of compounds is incorporated into the continuous phase while a second portion of the compound or a component of the mixture of compounds is incorporated into the dispersed phase.
- the first portion of the compound in the continuous phase is introduced to the microorganisms when the emulsion contacts the microorganisms, and the second portion of the compound is introduced after the microorganisms make contact with the dispersed phase.
- the compound stimulates the microorganisms to convert the carbonaceous material into one or more biogenic gases.
- FIG. 1 is a flowchart illustrating a method of dispersing a mixture within a geologic formation according to embodiments of the invention
- FIGS. 2A-C are flowcharts illustrating methods of producing a multiple emulsion incorporating a compound according to embodiments of the invention
- FIGS. 3A-C are flowcharts illustrating methods of producing an emulsion according to embodiments of the invention.
- FIG. 4 is a block diagram showing a process for creating a multiple emulsion according to embodiments of the invention.
- FIG. 5 shows a system for supplying an encapsulated compound to a formation environment according to embodiments of the present invention.
- FIG. 6 is a chart detailing various amendment compounds and methane produced over time in a controlled lability experiment.
- FIG. 7 is a chart detailing various amendment compounds and methane produced over time in a nutritional experiment including carbonaceous substrate.
- FIG. 8 is a chart detailing various amendment compounds and acetate turnover over time in a controlled lability experiment.
- FIG. 9 is a chart detailing various amendment compounds and acetate turnover over time in a nutritional experiment including carbonaceous substrate.
- FIG. 10 is a microscope image showing a view of the produced compounds after formation.
- FIG. 11 is a microscope image of the produced compounds one day after formation.
- FIG. 12 is a microscope image illustrating various of the produced emulsion types according to the present technology.
- emulsions may allow agents and nutrients to be dispersed over a larger portion of carbonaceous material located in the formation, and/or may allow a controlled release of the agents and nutrients over a longer period of time. This allows the agents and nutrients to be accessible to a larger number of microorganisms in the carbonaceous material, and for longer periods.
- one or more of the liquid phases may themselves constitute agents and/or nutrients for microorganisms.
- the stimulation effects of the agents and nutrients may include increasing the rate of production of the biogenic gas and/or an intermediary in a metabolic process that produces the gas.
- the effects may also include activating a consortium of microorganisms in the formation to start producing the biogenic gas. They may further include stopping or decreasing a “rollover” effect such as when the concentration of one or more metabolic products starts to plateau (or even drop) after a period of monotonically increasing.
- microorganisms may be provided in the mixtures themselves, and/or in separate solutions introduced to the geologic formation.
- the microorganisms may be provided to areas of the geologic formation (such as the carbonaceous material) that show little or no biological activity. They may also be provided to increase the microorganism population in areas where microorganisms are already present (e.g., where there is already a native microorganism population.)
- the added microorganisms may be selected to work in concert with the agent/nutrient mixture supplied to the formation.
- Compounds used in the methods described may act as nutrients, activation agents, initiators, or catalysts for increasing the production of biogenic gases including hydrogen and methane.
- the microorganisms may consume the compounds allowing the microorganism populations to grow more rapidly than without the compounds.
- activation agents the compounds may lower an activation barrier, open a metabolic pathway, modify a carbonaceous material, change the reaction environment, and may or may not be rapidly consumed as a nutrient.
- the compound When the compound is acting primarily like a nutrient, consumption of the nutrient by the microorganisms may increase the production of biogenic gas by a stoichiometrically proportional amount to that of the compound used.
- the increased amount of biogenic gas may be much larger than the amount of the compound added.
- introducing small quantities of the compound may produce much more than stoichiometric quantities of the biogenic gases.
- the compound acts as an activation agent, the compound may or may not act as a catalyst, and may be fully, partially, or not consumed while increasing the production of biogenic gas.
- the methods 100 may include accessing the geologic formation 110 .
- the geologic formation may be a previously explored, carbonaceous material-containing subterranean formation, such as a coal mine, oil field, natural gas deposit, carbonaceous shale deposit, etc.
- access to the formation may involve utilizing previously mined or drilled access points to the formation.
- accessing the formation may involve digging or drilling through a surface layer to access the underlying site.
- the geologic formations may include native carbonaceous materials that were formed in the formation through natural processes instead of being supplied to the formation through a human-directed process (e.g., dumping or pumping the carbonaceous material into the formation).
- Accessing the geologic formation 110 may also include accessing microorganisms present in the formation. These microorganisms may include methanogenic microorganisms that convert adjacent carbonaceous material into hydrogen (H 2 ), methane (CH 4 ), and/or other metabolic products that have hydrogen-to-carbon ratios higher than the starting carbonaceous material. The microorganisms may also include species of methanogenesis inhibitors that slow or inhibit methanogenic metabolic processes by consuming methanogenic precursors and/or producing compounds that inhibit methanogenesis.
- the method 100 may include a biological analysis 112 of the microorganisms. This may include a quantitative analysis of the population size determined by direct cell counting techniques, including the use of microscopy, DNA quantification, phospholipid fatty acid analysis, quantitative PCR, protein analysis, etc.
- the identification of the genera and/or species of one or more members of the microorganism consortium by genetic analysis may also be conducted. For example, an analysis of the DNA of the microorganisms may be done where the DNA is optionally cloned into a vector and suitable host cell to amplify the amount of DNA to facilitate detection.
- the detecting is of all or part of ribosomal DNA (rDNA), of one or more microorganisms.
- Detection may be by use of any appropriate means known to the skilled person.
- Non-limiting examples include restriction fragment length polymorphism (RFLP) or terminal restriction fragment length polymorphism (TRFLP); polymerase chain reaction (PCR); DNA-DNA hybridization, such as with a probe, Southern analysis, or the use of an array, microchip, bead based array, or the like; denaturing gradient gel electrophoresis (DGGE); or DNA sequencing, including sequencing of cDNA prepared from RNA as non-limiting examples.
- RFLP restriction fragment length polymorphism
- TRFLP terminal restriction fragment length polymorphism
- PCR polymerase chain reaction
- DNA-DNA hybridization such as with a probe, Southern analysis, or the use of an array, microchip, bead based array, or the like
- DGGE denaturing gradient gel electrophoresis
- DNA sequencing including sequencing of cDNA prepared from RNA as non-limiting examples.
- SC-WGS Single Cell Whole Genome Sequencing
- the isolation of hundreds of individual cells in a sample from the geologic formation may be achieved.
- Hundreds of individual cells may be isolated using fluorescence-activated cell sorting to separate and deposit each cell into its own well in a microtiter plate.
- the deposited cell may be lysed, its entire genome amplified then screened for identity using SSU rRNA census sequencing techniques and appropriate amplified genomes selected for genome sequencing.
- Gene annotation of SC-WGA samples can be done using the Integrated Microbial Genomes (IMG) and RAST databases to provide a comprehensive comparative analysis of putative gene function and uncover the dominant metabolic and degradation pathways for the most abundant and active bacteria within the samples. Additional details of the biological analysis of the microorganisms is described in co-assigned U.S.
- activation agents and nutrients may be provided that target particular microorganisms or metabolic pathways in order to stimulate or favor metabolism of the carbonaceous material to make particular metabolic products or biogenic gases.
- the method 100 may also include environmental analysis of the formation environment. For example, extracted geologic formation samples such as water, rock, and sediment bearing the carbonaceous material may be analyzed using spectrophotometry, NMR, HPLC, gas chromatography, mass spectrometry, voltammetry, and other chemical instrumentation. The tests may be used to determine the presence and relative concentrations of elements like dissolved carbon, phosphorous, nitrogen, sulfur, magnesium, manganese, iron, calcium, zinc, tungsten, cobalt, and molybdenum, among other elements.
- environmental analysis of the formation environment For example, extracted geologic formation samples such as water, rock, and sediment bearing the carbonaceous material may be analyzed using spectrophotometry, NMR, HPLC, gas chromatography, mass spectrometry, voltammetry, and other chemical instrumentation. The tests may be used to determine the presence and relative concentrations of elements like dissolved carbon, phosphorous, nitrogen, sulfur, magnesium, manganese, iron, calcium, zinc, tungsten, cobalt, and mo
- the analysis may also be used to measure quantities of polyatomic ions such as PO 2 3 ⁇ , PO 3 3 ⁇ , and PO 4 3 ⁇ , NH 4 + , NO 2 ⁇ , NO 3 ⁇ , and SO 4 2 ⁇ , among other ions.
- the quantities of vitamins, and other nutrients may also be determined.
- An analysis of the pH, salinity, oxidation potential (Eh), and other chemical characteristics of the formation environment may also be performed. Additional details of chemical analyses that may be performed are described in co-assigned PCT Application No. PCT/US2005/015259, filed May 3, 2005; and U.S. Pat. No. 7,426,960, filed Jan. 30, 2006, of which the entire contents of both applications are herein incorporated by reference for all purposes.
- a mixture may be provided 115 to stimulate the production of biogenic gas (e.g., methanogenesis).
- the mixture may take the form of an emulsion that incorporates one or more activation agents and/or nutrients that are provided to the geologic formation.
- Techniques for providing the mixture 115 may include direct injection processes that pump and/or pour the mixture into the formation environment.
- the mixture may be non-homogeneous or homogeneous, and may include multiple phases, including solid and liquid phases, and two or more liquid phases.
- Exemplary mixtures having two or more liquid phases may include emulsions that have one or more dispersed phases surrounded by a continuous phase.
- the lack of miscibility that causes the separate liquid phases may be due to different polarities of the liquids.
- a dispersed liquid phase may be non-polar while the continuous phase is polar.
- the dispersed phase may have a polar liquid while the continuous phase is made from a non-polar liquid.
- Exemplary emulsions may include oil-in-water (O/W) emulsions that have a non-polar dispersed phase incorporated into a continuous phase that include polar water molecules. They may also include water-in-oil (W/O) emulsions where droplets of polar water or an aqueous solution are dispersed in a non-polar hydrocarbon-containing continuous phase. The emulsions may be classified as microemulsions and/or nanoemulsions if the size of the dispersed-phase droplets are the requisite size.
- O/W oil-in-water
- W/O water-in-oil
- the emulsions may be classified as microemulsions and/or nanoemulsions if the size of the dispersed-phase droplets are the requisite size.
- the emulsion may be a single emulsion containing two-phases, such as O/W, or may alternatively, in some embodiments, be a multiple emulsion including an emulsion contained in a separate continuous phase, such as W/O/W for example.
- the mixture may further contain surfactants and/or emulisifiers that slow or prevent the dispersed phases from coagulating and/or forming a separate layer of the mixture.
- the activation agents and/or nutrients may be dissolved in the one or more dispersed phases, the continuous phase, or both.
- the agents/nutrients may be soluble in a polar solvent such as water (i.e., an aqueous phase), or a non-polar solvent such as found in an “oil” phase.
- a polar solvent such as water (i.e., an aqueous phase)
- a non-polar solvent such as found in an “oil” phase.
- the agents/nutrients that are soluble in the aqueous phase would be found in the continuous phase of (1), and dispersed phase of (2), respectively.
- Agents and nutrients that are at least partially soluble in both polar and non-polar solvents may be found in both the dispersed and continuous phases of the emulsions. Examples may further include agents and nutrients that are partially dissolved in one or more of the liquid phases of the emulsion, and that also have a solid phase component suspended and/or precipitated from the liquid phases.
- a non-polar “oil” phase may include compounds having at least a non-polar, hydrophobic/lipophilic moiety such as a long chain hydrocarbon.
- these non-polar compounds may include mineral oils, essential oils, and organic oils, among other types of oils. They may further include lipids and fatty acids that include non-polar, hydrophobic hydrocarbon tails.
- Exemplary fatty acids may include naturally occurring, saturated and/or unsaturated fatty acids such as myristoleic acid, palmitoleic acid, sapienic acid, oleic acid, linoleic acid, ⁇ -linolenic acid, arachidonic acid, eicosapentaenoic acid, erucic acid, docosahexaenoic acid, ricinoleic acid, etc., among other fatty acids having one or more double-bonds between carbon atoms in the hydrocarbon chains, and including configurations with hydrogen atoms being located on the same or opposite sides of the double bond, such as with cis- or trans-configurations of the acids.
- fatty acids such as myristoleic acid, palmitoleic acid, sapienic acid, oleic acid, linoleic acid, ⁇ -linolenic acid, arachidonic acid, eicosapentaenoic acid, erucic acid, docosahe
- Exemplary saturated fatty acids may include lauric acid, myristic acid, palmitic acid, stearic acid, arachidic acid, behenic acid, lignoceric acid, cerotic acid, etc., among other fatty acids that have no double bonds and are saturated with hydrogen atoms.
- Some of the possible combinations of acids that may be used in the non-polar phase may be from naturally occurring fats and oils, and may include animal fats including lard, duck fat, butter, as well as vegetable fats including coconut oil, palm oil, cottonseed oil, wheat germ oil, soya or soybean oil, olive oil, corn oil, sunflower oil, safflower oil, hemp oil, canola oil, among others.
- Other possible combinations may include engineered formulations that have been shown to remain dispersed in oil-in-water emulsions for particular periods of time without creaming or separation of the dispersed non-polar phase.
- Polar phase compounds may include water, or aqueous solutions incorporating salts, sugars, proteins, amino acids, chlorogenic acids, protein hydrolyzates, various cell extracts such as yeast extract, algal extracts, or other compounds including volatile fatty acids, or acids such as acetic acid, propionate, butyrate, oxyphosphorous acids, etc.
- Additional exemplary polar phase compounds may include formamide, dimethyl sulfoxide, and ferulic acid among others.
- the included algal extracts may include Chlamydomonaas spp., or Spirulina spp., for example.
- a compound that acts as a nutrient or activation agent may be contained within one or more phases of the mixture that is provided 115 to the geologic formation.
- the compound may stimulate the microorganisms to metabolize carbonaceous material in the formation into biogenic gas, such as methane, or into intermediate metabolites that may further be metabolized into biogenic gases.
- the activation agent may contain various compounds for stimulating microorganisms in the formation environment. Activation agents provided may include phosphorous compounds, acetate compounds, or other nutrients for the microorganisms.
- the phosphorus compounds may include phosphorus compounds (e.g., PO x compounds were x is 2, 3 or 4), such as sodium phosphate (Na 3 PO 4 ) and potassium phosphate (K 3 PO 4 ), as well as monobasic and dibasic derivatives of these salts (e.g., KH 2 PO 4 , K 2 HPO 4 , NaH 2 PO 4 , Na 2 HPO 4 , etc.). They may also include phosphorus oxyacids and/or salts of phosphorus oxyacids.
- phosphorus compounds e.g., PO x compounds were x is 2, 3 or 4
- sodium phosphate Na 3 PO 4
- K 3 PO 4 potassium phosphate
- monobasic and dibasic derivatives of these salts e.g., KH 2 PO 4 , K 2 HPO 4 , NaH 2 PO 4 , Na 2 HPO 4 , etc.
- They may also include phosphorus oxyacids and/or salts of phosphorus
- the phosphorus compounds may include H 3 PO 4 , H 3 PO 3 , and H 3 PO 2 phosphorus oxyacids, as well as dibasic sodium phosphate and dibasic potassium phosphate salts.
- the phosphorus compounds may also include alkyl phosphate compounds (e.g., a trialkyl phosphate such as triethyl phosphate), and tripoly phosphates.
- the phosphorus compounds may further include condensed forms of phosphoric acid, including tripolyphosphoric acid, and pyrophosphoric acid, among others.
- They may also include the salts of condensed phosphoric acids, including alkali metal salts of tripolyphosphate (e.g., potassium or sodium tripolyphosphate), among other salts, and may also include oxides of phosphorus (e.g., phosphorus trioxide, pentoxide, etc.), among other compounds.
- alkali metal salts of tripolyphosphate e.g., potassium or sodium tripolyphosphate
- oxides of phosphorus e.g., phosphorus trioxide, pentoxide, etc.
- Examples of the acetate compounds may include acetic acid, and/or an acetic acid salt (e.g., an alkali metal salt of acetic acid, an alkali earth metal salt of acetic acid, sodium acetate, potassium acetate, etc.), among other acetate compounds.
- Other activation agents that may be provided include nutrients such as yeasts and yeast extracts, and may include digests and extracts of commercially available brewers and bakers yeasts.
- Other activation agents may include nutrients including carboxylate compounds, proteins, hydrogen release compounds, minerals, metal salts, and/or vitamins, among other components.
- Catalysts may also be provided in the emulsions to activate the microorganisms or particular metabolic pathways.
- Still other compounds that may be included in the emulsion include cyclic and aromatic compounds that may include either or both of an ether linked group and an ester linked group, and may include vanillin and syringic acid, among other compounds and acids with a phenol group or other aryl group, and functional groups that may include, in some embodiments, hydroxyl groups, carboxylates, aldehydes, ethers, esters, etc., among others.
- the emulsion may be provided 115 in several separate applications over time as opposed to a single application. These separate applications may provide multiple stages of activation or stimulation over a period of time that may be monitored either through the rate of production of biogenic gas, or alternatively through in situ measurements of the concentrations of the activation agent or a metabolic byproduct produced during the conversion of the carbonaceous material. Additionally, these introductions may be made to the formation over the course of the activation period to maintain a certain concentration level or range of the activation agent in the formation.
- the mixture may be dispersed through the geologic formation 120 .
- the dispersion of the mixture may be done using a variety of techniques, such as high-pressure pumping that forces the mixture to permeate the formation environment.
- the dispersion pattern of a mixture's agents and/or nutrients with respect to the formation and the carbonaceous material may be based at least in part on how the liquid phases are emulsified and how the agents/nutrients are dissolved in the liquid phases. For example, in a mixture that is an oil-in-water emulsion having the agent/nutrient dissolved in the non-polar, dispersed “oil” phase, the agent/nutrient may be transported further by the aqueous continuous phase across the carbonaceous material before being absorbed.
- the distribution of the non-polar dispersed phase may be more localized along the boundary of the formation water and carbonaceous material. It may also distribute the dispersed phase over a larger area of the carbonaceous material at the water-material boundary. In this example, more agent/nutrient may be supplied to the carbonaceous material by localizing the non-polar dispersed phase close to the surface of the material instead of diluting it throughout the adjacent formation water.
- the mixture may make direct contact with carbonaceous material and/or indirect contact by first being dispersed in the formation before reaching the carbonaceous material.
- the microorganisms may be found in a variety of locations in the geologic formation, including the carbonaceous material and/or the formation water, among other locations.
- the mixture may be dispersed within the geologic formation 120 in order to reach the microorganisms that may then be stimulated by the activation agent.
- the microorganisms may access the activation agent directly, or in some embodiments, may consume the non-polar dispersed phase to access the activation agent. In consuming or metabolizing the non-polar dispersed phase, the microorganisms may be provided with an additional nutrient source in addition to the activation agent.
- Metabolizing the non-polar dispersed phase may produce an acetate compound that may be utilized by the same or different microorganisms within the formation environment as an additional nutrient source to stimulate the microorganisms. Metabolizing the non-polar dispersed phase may also produce hydrocarbons that are similar in nature and structure to the carbonaceous material being converted by the microorganisms, and may produce metabolic products including biogenic gases, among other products.
- Method 100 may also include measuring the rate of biogenic gas production 125 .
- the partial pressure of the product in the formation may be measured. Measurements may also be made before providing the activation agent, and a comparison of the product concentration before and after the activation agent may also be made.
- the biogenic gas products may also be recovered from the formation environment, or maintained within the formation environment at a concentration range that has been found to stimulate the microorganisms to generate more of the biogenic gas product. Based on the rate of biogenic gas production, more or less activation agents or nutrients may be added to the geologic formation in order to maximize biogenic gas output.
- FIG. 2A shows a flowchart illustrating a method 200 of producing a multiple emulsion incorporating one or more stimulating compounds according to embodiments of the invention.
- the method 200 may include providing both a polar aqueous fluid and a non-polar fluid 210 .
- the non-polar fluid may be any of the previously mentioned oils or fatty acids, and may additionally be a fluid at least partially immiscible in water.
- the added compound may be combined with a first portion of the polar fluid 212 .
- the compound may be dissolved, mixed, suspended, or otherwise contacted with the fluid.
- the method 200 may also include adding an oil-soluble surface active agent (“surfactant”), and/or emulsifier to the non-polar fluid.
- surfactant oil-soluble surface active agent
- the surfactant may be chosen based on its hydrophilic-lipophilic balance (“HLB”) number, which is an indication of the hydro- or lipophilicity of the surfactant.
- HLB hydrophilic-lipophilic balance
- a higher HLB value describes a compound that is more hydrophilic, while a lower HLB value describes a compound that is more lipophilic.
- the surfactant used in the emulsion may have a lower HLB value, thus being more lipophilic, and exemplary surfactants may have HLB values below or about 10, below or about 8, below or about 6, below or about 4, or below or about 2.
- the method 200 may include producing a primary emulsion by adding the portion of the polar fluid containing the nutrient to the non-polar fluid with the surfactant 214 .
- the emulsion may be produced by agitating the combined fluids in a blender, magnetic mixer, or another device that applies relatively high shear to the emulsion components.
- the emulsion may be further stabilized by processing the emulsion in a colloid mill or homogenizer, or a device that applies a very high level of hydraulic shear to the emulsion.
- This produced emulsion may have macro, micro, or nano-sized particles depending on the requirements of the particular application.
- the emulsion may also be produced in a device that raises the temperature of the components above room temperature during the processing. Raising the temperature of the emulsion may reduce the viscosity of the dispersed phase which may facilitate emulsification of the components.
- the temperature of the components may be raised during the processing to above or about 25° C., above or about 50° C., above or about 70° C., or above or about 90° C.
- the method 200 may further include making a multiple emulsion.
- a second portion of a polar fluid which may include a surfactant and/or emulsifier, may be used as a continuous phase in the multiple emulsion.
- the surfactant may have a higher HLB value, making it more hydrophilic.
- the surfactant may have an HLB value above or about 8, above or about 10, above or about 13, above or about 15, or above or about 18.
- the method 200 may also include adding the primary emulsion to the second portion of the polar fluid with the surfactant to produce a multiple emulsion 216 .
- the multiple emulsion may be produced by agitating the combined fluids using a relatively low shear device to avoid separating the primary emulsion.
- a blender or magnetic mixer may be utilized to produce both the primary emulsion and the secondary emulsion.
- the speed used for the secondary emulsion may be lower than the speed used for the primary emulsion, and may be, for example, 50% or less of the speed used in the primary emulsion.
- the degree of shear used in the preparation of the primary and secondary emulsion may vary depending on the polar and non-polar fluids and surfactants used, as well as the types of encapsulated compounds.
- a high-shear device may be used to produce the primary emulsion, such as a high-pressure homogenizer.
- a low-shear device may be used to create the secondary emulsion, such as a combined orifice device, or a device utilizing a membrane emulsification technique.
- the osmotic gradient between the two portions of the aqueous fluids may be maintained at a low-enough threshold such that the encapsulated aqueous portion does not pass through the non-polar fluid phase into the continuous aqueous phase.
- the osmotic pressure between the two aqueous phases may be adjusted by changing the concentrations of the fluids, which may be done, for example, with the addition of the surfactants, or alternatively by adding various salts, sugars, sugar esters, and/or other compounds.
- the surfactants used in the emulsions may be selected based on the compounds provided to the carbonaceous material.
- Surfactants used may include cationic surfactants, such as benzalkonium chloride; anionic surfactants, such as alkali or amine soaps, and detergents; and/or Zwitterionic surfactants, such as CHAPS or sultaines, amino and imino acids, or phosphates such as lecithin.
- the emulsions may also include nonionic emulsifying agents.
- Nonionic emulsifiers that may be used in either of the primary emulsion or the secondary emulsion may include, for example, polymeric and non-polymeric compounds, as well as fatty alcohols, such as cetyl alcohol, stearyl alcohol, cetostearyl alcohol, and oleyl alcohol, among others.
- nonionic surfactants that may be used in the formation of the emulsions include glyceryl monostearate, polyoxyethylene monooleate or monostearate or monolaurate, and other polyoxyethylene glycol alkyl ethers (PEG-400 emulsifiers), polyoxypropylene glycol alkyl ethers, glucoside alkyl ethers, potassium oleate, sodium lauryl sulfate, and sodium oleate, among others.
- PEG-400 emulsifiers polyoxyethylene glycol alkyl ethers
- polyoxypropylene glycol alkyl ethers polyoxypropylene glycol alkyl ethers
- glucoside alkyl ethers potassium oleate
- sodium lauryl sulfate sodium oleate
- nonionic emulsifiers that may be used in the formation of the emulsions may include sorbitan alkyl esters (Spans), including sorbitan monolaurate (Span 20), sorbitan monopalmitate (Span 40), sorbitan monostearate (Span 60), sorbitan tristearate (Span 65), sorbitan monooleate (Span 80), sorbitan sesquioleate (Span 83), and sorbitan trioleate (Span 85), among others.
- sorbitan alkyl esters sorbitan monolaurate (Span 20), sorbitan monopalmitate (Span 40), sorbitan monostearate (Span 60), sorbitan tristearate (Span 65), sorbitan monooleate (Span 80), sorbitan sesquioleate (Span 83), and sorbitan trioleate (Span 85), among others.
- nonionic surfactants that may be used in the formation of the emulsions may include triethanolamine oleate, polyoxyethylene derivatives of sorbitan alkyl esters (Tweens), including polyoxyethylene sorbitan monolaurate (Tween 20), polyoxyethylene sorbitan monolaurate (Tween 21), polyoxyethylene sorbitan monopalmitate (Tween 40), polyoxyethylene sorbitan monostearate (Tween 61), polyoxyethylene sorbitan tristearate (Tween 65), polyoxyethylene sorbitan monooleate (Tween 80), polyoxyethylene sorbitan monooleate (Tween 81), and polyoxyethylene sorbitan trioleate (Tween 85), among others.
- Tweens polyoxyethylene derivatives of sorbitan alkyl esters
- Tweens polyoxyethylene derivatives of sorbitan alkyl esters
- Tweens polyoxyethylene derivatives of sorbitan alkyl est
- the emulsions may also incorporate natural emulsifying agents, and/or hydrocolloids.
- the emulsions may include vegetable derivatives, such as acacia, tragacanth, agar, pectin, carrageenan, lecithin, and others; or animal derivatives, such as gelatin, lanolin, and cholesterol, among others.
- Semi-synthetic agents such as methylcellulose, and carboxymethylcellulose, and fully synthetic agents such as Carbopols may also be used.
- Solid particle emulsifiers may be used to form a particulate layer around the dispersed phase. These agents may include dispersed particles of bentonite, veegum, hectorite, magnesium hydroxide, magnesium trisilicate, and aluminum hydroxide, among others.
- the method 200 may also include transporting the emulsion to the geologic formation 218 .
- the transporting may be done in such a way as to maintain the stabilized emulsion, such as by utilizing transportation equipment and vehicles that will minimize changes in temperature or pressure on the emulsion.
- the emulsion may be made at the site of the geologic formation where the compound may be injected, such that transportation may not be a concern. Where the emulsion must be transported over long distances, additional surfactants, mixing transportation vessels, or different amounts of the surfactants used in the preparation of the emulsion may be utilized to produce a stable emulsion.
- the emulsion may be injected into the geologic formation 220 to contact the carbonaceous material with the encapsulated compound.
- the injection may include a pumping mechanism to force the emulsion into the geologic formation, and disperse the emulsion over a greater area of the formation.
- the emulsion may be poured into the formation and allowed to remain at the site of the injection.
- the non-polar fluid may allow the activation agent to disperse over a larger area because the non-polar fluid may not be absorbed into the carbonaceous material, formation water, or the formation itself.
- a method 230 is described in which a paused or staged dispersion may be created from the ways in which the nutrients or other compounds are incorporated within the emulsion.
- the method 230 may provide a polar and a non-polar fluid 240 as previously described.
- the activation agent or nutrient compound may be disposed 242 within the polar fluid. Portions of the polar fluid containing the compound may be used both within the non-polar phase of the mixture in the first emulsion produced 244 , as well as for the continuous phase used for the multiple emulsion 246 . This emulsion may then be transported to the geologic formation 248 .
- Microorganisms may access this portion of the activation agent directly when the mixture has been injected 250 into the carbonaceous material.
- the non-polar phase may be consumed by the microorganisms as a nutrient source, and the consumption of the non-polar phase may release the portion of the activation agent or nutrient contained therein.
- the release of this portion of the activation agent may occur at a time later than that at which the portion of the activation agent disposed within the continuous aqueous phase was consumed.
- a paused or delayed dispersion may allow greater amounts of nutrients or activation agents to be delivered at lower concentrations over longer periods of time to the carbonaceous material than could otherwise be provided.
- Adding large amounts of a specific compound to the geologic formation in one dose may in some circumstances create deleterious effects including the death or deactivation of microorganisms.
- an overall greater amount of the compound may be used in a single injection.
- the larger amounts may allow fewer applications of the activation agents to the geologic formation, which may provide for improved consistency of the applications to create a more controlled stimulation of the microorganisms within the formation.
- Paused or staged dispersion may allow time for the microorganisms to grow or activate before the additional portion of the activation agent may be released.
- a greater portion of the activation agent may be contained in the non-polar dispersed phase than in the aqueous continuous phase.
- the non-polar dispersed phase may be consumed as a nutrient by the microorganisms, the consumption of which may release equivalent or greater portions of the activation agent.
- This second dosing with larger amounts of activation agent may be more effectively utilized by a larger and/or more robust microorganism population that has been given time to grow and acclimate to metabolizing coal carbon to new biogenic methane gas.
- a greater portion of the activation agent may be contained in the polar continuous phase of the emulsion, and a lesser amount within the non-polar dispersed phase.
- the lesser amount of the activation agent may be utilized to maintain the activation of the microorganisms during their metabolizing of the carbonaceous material.
- a method 260 is described in which a multiple emulsion may be created that contains both an activation agent and a nutrient.
- the microorganisms may be capable of increased growth and ability to convert carbonaceous material due to a compound effect provided by the combination of elements.
- the method 260 may include the step of providing a polar and non-polar fluid 270 , substantially as described previously.
- a nutrient may be combined 274 within a first portion of a polar fluid, and an activation agent may be combined 276 with a second portion of the polar fluid.
- An emulsion may be produced 278 within the non-polar fluid with the portion of the polar fluid containing the nutrient.
- the portion of the polar fluid containing the activation agent may then be used as the continuous phase of a multiple water-in-oil-in-water emulsion 280 containing the emulsion produced with the nutrient as the dispersed phase.
- the emulsion may then be transported to the geologic formation 282 , and when introduced to a carbonaceous material 284 , the activation agent located in the continuous aqueous phase may stimulate the microorganisms to metabolize the carbonaceous material.
- the microorganisms may either consume as a nutrient source, or surpass due to separation, the non-polar dispersed phase, thereby releasing the nutrient contained therein for consumption by the microorganisms.
- FIGS. 3A-C show methods of forming a single emulsion including a polar fluid and a non-polar fluid.
- FIG. 3A shows a method 300 in which a polar and non-polar fluid may be provided 310 , and a compound may be incorporated into the polar fluid 312 .
- the polar fluid may be dispersed within the non-polar fluid producing an emulsion 314 .
- This water-in-oil type emulsion may then be directly injected into the geologic formation to contact the carbonaceous material.
- the non-polar continuous phase may be consumed or metabolized by the microorganisms as a nutrient source.
- the metabolic products created by the breakdown of the non-polar continuous phase may include acetate compounds, as well as other hydrocarbons including biogenic gases, among other compounds. Metabolizing the non-polar continuous phase may release the nutrient or activation agent contained in the polar aqueous phase, which may be further consumed to stimulate the microorganisms to convert the carbonaceous material into biogenic gas.
- a method 320 is described that also may provide a polar and non-polar fluid 330 .
- the compound may be combined with the non-polar fluid 332 , which may then be encapsulated in a polar aqueous fluid 334 and injected into a geologic formation as described previously.
- a compound may be disposed in at least one or both of the emulsion phases, which may allow a staged or paused release of the compound to the microorganisms within the formation as discussed above.
- a method 340 may provide a polar and non-polar fluid 350 , and may include suspending microorganisms within the polar fluid 352 .
- This polar fluid may then be encapsulated within a non-polar fluid to produce an emulsion 354 .
- This emulsion may be combined with a polar fluid to produce a multiple emulsion, or alternatively, may be injected directly into the geologic formation.
- the polar aqueous continuous phase of the multiple emulsion may additionally contain other nutrients or activation agents to be consumed by the encapsulated microorganisms or by microorganisms native to the formation.
- the geologic formation environment may be anaerobic, and thus, the production and transportation of the emulsions may occur under anaerobic conditions.
- the encapsulated microorganisms may be contained in an anaerobic aqueous fluid used in the primary emulsion.
- Anaerobic fluid is characterized as having little or no dissolved oxygen, in general no more than 4 mg/L, preferably less than 2 mg/L, and most preferably less than 0.1 mg/L, as measured at 20° C. and 760 mmHg barometric pressure.
- the microorganisms may be from the same geologic formation to which they will be injected, and are being reinjected in order to be dispersed to alternative portions of the geologic formation, or to a broader area of the geologic formation.
- the microorganisms may be from a different geologic formation and are being transported to the geologic formation containing the carbonaceous material sought to be converted into biogenic gas.
- the microorganisms may have been modified, for example genetically, prior to their being injected or reinjected into the geologic formation.
- equipment and vehicles that are oxygen impermeable may be used, otherwise the microorganisms may be damaged in the process.
- FIG. 4 shows a block diagram for selected components of a system 400 for creating a multiple emulsion according to embodiments of the invention.
- Low-HLB surfactant 411 may be incorporated into non-polar fluid 412 that may be added to mixing vessel 410 .
- Activation agent 403 may be incorporated into polar aqueous fluid 407 , that may then be incorporated into non-polar fluid 412 in mixing vessel 410 to create a primary emulsion.
- Mixing vessel 410 may be, among other equipment, a high-shear mixer, blender, or mortar that may create macro, micro, and/or nanoemulsions.
- the primary emulsion may be further stabilized and refined in high-shear device 420 , such as a high-pressure homogenizer, or a colloid mill through which the primary emulsion may be passed.
- high-shear device 420 such as a high-pressure homogenizer, or a colloid mill through which the primary emulsion may be passed.
- Either or both of the mixing vessel 410 and the high-shear device 420 may be heated to a temperature to facilitate the production of the primary emulsion.
- Alternative embodiments may include using only one of the mixing vessel 410 and high-shear device 420 .
- High-HLB surfactant 421 may be incorporated into polar aqueous fluid 422 in low-shear device 430 .
- the primary emulsion may be added to the polar aqueous fluid 422 in low-shear device 430 to create a multiple emulsion 440 .
- Low-shear device 430 may include a blade-type or magnetic mixer or blender that operates at a speed lower than mixing vessel 410 .
- low-shear device 430 may include a membrane through which the primary emulsion may be passed or pressed. Continuous phase, polar aqueous fluid 422 may be flowed across the membrane of the low-shear device separating droplets of the primary emulsion and creating a secondary, multiple emulsion 440 .
- FIG. 5 shows selected components of a system 500 for supplying an encapsulated nutrient to a formation environment according to embodiments of the present invention.
- Multiple emulsion 440 may be produced at geologic formation 510 .
- the multiple emulsion 440 may be transported to the geologic formation 510 or created at the site of the geologic formation 510 .
- Multiple emulsion 440 may be injected into geologic formation 510 via pipes or wells 520 either previously located in or presently placed in an access point in the geologic formation 510 .
- Pumping mechanism 535 may also be utilized to inject the multiple emulsion into the geologic formation 510 to disperse the multiple emulsion 440 over a greater area of the geologic formation.
- pumping mechanisms may transport make up water to the well head that may gravity feed into the well 520 .
- a separate pumping mechanism may supply a steady stream rate of emulsion 440 to the gravity feed water stream going into well/pipes 520 .
- system 500 may include transferring multiple emulsion 440 into a vehicle 540 to be delivered to one or more locations at a geologic formation 510 .
- Vehicle 540 may transport the multiple emulsion 440 to alternative geologic formations.
- oxygen impermeable pipes 530 and vehicles 540 may be utilized to prevent the introduction of oxygen to the emulsion 440 .
- emulsifiers Prior to transportation in vehicle 540 , additional emulsifiers, surfactants, or stabilizers may be added to the multiple emulsion 440 in order to facilitate transporting the emulsion in its dispersed form, and to resist separation of the phases.
- vehicle 540 arrives at the geologic formation at which the multiple emulsion 440 is to be introduced, multiple emulsion 440 may be added to mixing device 550 to more uniformly disperse any of the primary emulsion that has separated from multiple emulsion 440 .
- Microcosms tests were performed using fresh coal bed methane water from the Powder River Basin with and without 0.5 grams of ground Anderson coal.
- Emulsified oil amendment was tested for its ability to activate methanogenic coal conversion and compared to various nutritional controls.
- a microscopic examination of samples from methanogenically active microcosms tests showed the presence of emulsified oil micelles at significantly reduced particle numbers at 150 days after test initiation.
- FIG. 6 is a chart detailing various amendment compounds and methane produced over time in a controlled lability experiment.
- no carbonaceous material i.e. coal
- emulsion based amendments were provided in addition to standard amendments as well as slow-release lactate amendments.
- the two amendments of emulsified oil produced significantly more methane at faster rates over the experimental period than did any other amendment.
- fatty acids of the emulsified oils undergo metabolism through beta oxidation by which acetate units are cleaved from the long chain hydrocarbons.
- the derived acetate can be utilized as an activation agent to stimulate the microorganisms in the production of methane.
- the emulsions that also contained a phosphate and yeast extract amendment produced substantially more methane over the course of the procedure. This may provide both the activation potential of the acetate source as well as the nutrients that can be utilized over a longer period of time due to the encapsulation.
- FIG. 8 illustrates acetate turnover over time in an additional lability experiment performed with similar amendment samples.
- the emulsified oil based samples produced acetate that was initially accumulated in the system. However, instead of producing a build-up of metabolic product, the released acetate was consumed over a course of time for the experiment.
- the microorganism consortium may consume the released acetate after metabolizing the emulsified oil.
- microorganisms that may better utilize the acetate such as acetoclastic methanogens, may flourish as a result of the acetate available and grow to a more significant portion of the consortium.
- the emulsified oil including the phosphate and yeast extract produced a greater initial amount of acetate in the system followed by a faster utilization of the produced acetate by the consortium.
- FIGS. 7 and 9 together indicates that the acetate accumulated initially is consumed, followed by enhanced production of methane.
- FIG. 9 illustrates that after an initial accumulation of acetate during the first 50 days, the acetate is consumed. This consumption correlates to the increase of methane production beginning at roughly day 50 of FIG. 7 .
- This phenomenon may be a result of acetoclastic methanogenic organisms increasing in population followed by a period of activity during which the organisms consume the acetate thereby producing methane.
- the available acetate may activate the growth of the consortium, and release of the phosphate and yeast extract when the emulsified oil is metabolized may provide nutrients for stimulating methane production.
- the encapsulated nutrients may be utilized in an improved fashion as compared to a bolus of nutrients as they may be consumed over a longer period of time due to the encapsulation.
- less of the nutrients may be needed due to a more efficient utilization by the consortium.
- PGPR Polyglycerol polyricinoleate
- Xanthan gum was blended into a solution of yeast extract, acetate, and phosphate at 0.2% by weight to make a second solution.
- Equal parts of the first and second solutions were blended in bursts at high speed (>5000 RPM) until a viscous product was formed as a third product.
- FIG. 10 shows a microscope image of the formed emulsions at 400 ⁇ magnification directly after production.
- the graph shows formed emulsion droplets at 5 ⁇ m and 20 ⁇ m respectively as indicated, and shows the incorporation of yeast extract, acetate, and phosphate solution within the oil solution.
- FIG. 11 shows the same solution after 24 hours has elapsed showing that the yeast extract, acetate, and phosphate droplets have coalesced into cores of agent within the oil droplets.
- FIG. 12 shows additional views of emulsion species located within certain mixtures of the present technology.
- Droplets 1210 show large cores of the extract solution that are coalesced within the oil droplets.
- Droplets 1220 show water-in-oil nanodroplets that are dispersed throughout the mixture.
- Droplets 1230 show WOW particles produced in the mixture.
- one environment may include wireless control of processes or machinery from a remote terminal that can provide automatic instruction, while another environment may include no such control and is operated locally at the site of the process based on then current operation conditions.
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Abstract
Description
- This application claims the benefit of U.S. Provisional Application No. 61/613,380, filed Mar. 20, 2012, entitled “Dispersion of Compounds for the Stimulation of Biogenic Gas Generation in Deposits of Carbonaceous Material.” The entire disclosure of which is incorporated herein by reference for all purposes.
- As the price of oil rises, along with economic and environmental pressures to find local and alternative energy sources, the use of natural gas as a safe and reliable energy source continues to grow. Natural gas is used as an energy source for heating, electric power generation, and transportation fuel. Natural gas is also used for the production of hydrogen, and in many manufacturing processes.
- The majority of natural gas is found in underground deposits, many of which are the same geologic formations that contain liquid and solid carbonaceous material such as oil fields and coal beds. Much of the production of natural gas is believed to occur by biogenic processes, such as by methanogenic microorganisms that exist in the geologic formations and metabolize the carbonaceous material into substances such as natural gas as a metabolic product. The work of these microorganisms over thousands and millions of years has produced deposits of natural gas that measure in the trillions of cubic feet.
- As natural gas use increases globally, these reserves will be depleted creating new types of energy crises. Fortunately, the same biogenic processes that originally produced many of these deposits may be utilized to continue producing natural gas on a globally significant scale. Furthermore, if biogenic processes may be improved or enhanced to convert even a small fraction of the carbonaceous material in current formations to natural gas, the quantities produced could be enormous. For example, the Powder River Basin in northeastern Wyoming is estimated to contain over 1 trillion short tons of coal. If even 1% of this coal could be converted to natural gas, it could supply the current annual natural gas usage in the United States (about 23 trillion cubic feet) for four years. Many previously mined coal and oil fields in the United States alone that have become economically prohibitive to continued mining still contain these quantities of residual carbonaceous materials.
- Among the challenges faced in the biogenic conversion of these carbonaceous materials to natural gas and other hydrocarbons is providing an ample and continued source of nutrients and activation agents to the microorganisms that metabolize the carbonaceous materials. The standard spacing of wells for natural gas production is typically forty or eighty acre spacing (i.e., 1 well/40 acres 1 well/80 acres). Due to the depths and spacing of these wells, as well as the underground distances covered by the formations, gas produced through methanogenesis may take a substantial amount of time to travel to the surface for collection. Nutrients and activation agents that have been introduced into the formation to activate or stimulate methanogenesis may be exhausted long before it has had sufficient opportunity to be widely distributed by the advective dispersion processes within the formation. This may slow, or even prevent, the enhanced conversion of carbonaceous material into natural gas, because only a small portion of the subsurface microorganism populations' growth and activity.
- Thus, there is a need for improved nutrients and activation agents for stimulating the biogenic production of natural gas and other metabolic products. There is also a need for improved ways of delivering nutrients and activation agents into the formation environment. These and other needs are addressed by the present invention.
- Additional production of biogenic gases from carbonaceous materials found in geologic formations may be realized by supplying mixtures to microorganisms in the formations that stimulate their conversion of the carbonaceous materials to gases like hydrogen and methane. The mixtures may include one or more liquid dispersed phases surrounded by a liquid continuous phase that is characteristic of an emulsion. The mixtures may also include solid phase materials characteristic of a suspension. The compounds in the mixtures that stimulate biogenic gas production from the microorganisms may include activation agents and/or nutrients. These compounds may be at least partially dissolved in one or more liquid phases of the mixture that is supplied to the microorganisms. In some instances the materials that form a liquid phase (e.g., a dispersed phase) may also act as a nutrient or activation agent for the microorganisms.
- Exemplary mixtures may include oil-in-water and water-in-oil emulsions. The “oil” phase may be made of a non-polar liquid at the working temperatures of the mixture, and the “water” phase may be made from a polar liquid such as water or an aqueous solution, though other polar liquids may be used instead of (or in addition to) water. Exemplary mixtures may also include more complex multiple emulsions where the dispersed phase includes two or more liquid phases that are not completely miscible. Examples of these multiple emulsions may include water-in-oil-in-water (W/O/W) emulsions which have a dispersed phase that includes a core polar “water” droplet surrounded by a non-polar “oil” droplet surrounded by a polar “water” continuous phase.
- Embodiments of the invention may include methods of dispersing an activation agent to a carbonaceous material to stimulate production of a biogenic gas. The methods may include accessing a subterranean geologic formation containing the carbonaceous material, and supplying a mixture to the formation. The mixture may include the activation agent mixed with a dispersed phase and a continuous phase. The method may also include contacting the carbonaceous material with the mixture, and distributing at least a portion of the activation agent over and/or into the carbonaceous material from the dispersed phase. The production of biogenic gases is increased by microorganisms that are stimulated by the distributed activation agent to convert a portion of the carbonaceous material into the biogenic gases.
- Embodiments of the invention may also include methods of providing a nutrient to a carbonaceous material to stimulate production of biogenic gas from the material. The methods may include accessing a geologic formation containing the carbonaceous material, and delivering a mixture to the formation that includes a dispersed phase, a continuous phase, and the nutrient incorporated into at least one of these phases. The mixture may contact the carbonaceous material and become available to microorganisms in proximity to the material. The nutrient stimulates the microorganisms to convert at least a portion of the carbonaceous material into biogenic gas, increasing the production of the biogenic gas.
- Embodiments of the invention may still further include methods of introducing multiple portions of a compound or a mixture of compounds to microorganisms in a geologic formation. The methods may include accessing a geologic formation containing carbonaceous material, and supplying an emulsion to the formation. The emulsion may have a continuous phase and a dispersed phase, and a first portion of the compound or a single component of a mixture of compounds is incorporated into the continuous phase while a second portion of the compound or a component of the mixture of compounds is incorporated into the dispersed phase. The first portion of the compound in the continuous phase is introduced to the microorganisms when the emulsion contacts the microorganisms, and the second portion of the compound is introduced after the microorganisms make contact with the dispersed phase. The compound stimulates the microorganisms to convert the carbonaceous material into one or more biogenic gases.
- Additional embodiments and features are set forth in part in the ensuing detailed description and accompanying drawings, and in part will become apparent to those skilled in the art upon examination of the specification, or may be learned by the practice of the invention. The features and advantages of the invention may be realized and attained by means of the instrumentalities, combinations, and methods described in the specification.
- The present invention is described in conjunction with the appended figures:
-
FIG. 1 is a flowchart illustrating a method of dispersing a mixture within a geologic formation according to embodiments of the invention; -
FIGS. 2A-C are flowcharts illustrating methods of producing a multiple emulsion incorporating a compound according to embodiments of the invention; -
FIGS. 3A-C are flowcharts illustrating methods of producing an emulsion according to embodiments of the invention; -
FIG. 4 is a block diagram showing a process for creating a multiple emulsion according to embodiments of the invention; -
FIG. 5 shows a system for supplying an encapsulated compound to a formation environment according to embodiments of the present invention. -
FIG. 6 is a chart detailing various amendment compounds and methane produced over time in a controlled lability experiment. -
FIG. 7 is a chart detailing various amendment compounds and methane produced over time in a nutritional experiment including carbonaceous substrate. -
FIG. 8 is a chart detailing various amendment compounds and acetate turnover over time in a controlled lability experiment. -
FIG. 9 is a chart detailing various amendment compounds and acetate turnover over time in a nutritional experiment including carbonaceous substrate. -
FIG. 10 is a microscope image showing a view of the produced compounds after formation. -
FIG. 11 is a microscope image of the produced compounds one day after formation. -
FIG. 12 is a microscope image illustrating various of the produced emulsion types according to the present technology. - In the appended figures, similar components and/or features may have the same numerical reference label. Further, various components of the same type may be distinguished by following the reference label by a letter that distinguishes among the similar components and/or features. If only the first numerical reference label is used in the specification, the description is applicable to any one of the similar components and/or features having the same first numerical reference label irrespective of the letter suffix.
- Methods, systems and compositions are described for stimulating production of biogenic gases in geologic formations with activation agent and/or nutrient mixtures that may include two or more liquid phases that are not fully miscible (e.g., emulsions). The emulsions may allow agents and nutrients to be dispersed over a larger portion of carbonaceous material located in the formation, and/or may allow a controlled release of the agents and nutrients over a longer period of time. This allows the agents and nutrients to be accessible to a larger number of microorganisms in the carbonaceous material, and for longer periods. In some instances, one or more of the liquid phases may themselves constitute agents and/or nutrients for microorganisms.
- The stimulation effects of the agents and nutrients may include increasing the rate of production of the biogenic gas and/or an intermediary in a metabolic process that produces the gas. The effects may also include activating a consortium of microorganisms in the formation to start producing the biogenic gas. They may further include stopping or decreasing a “rollover” effect such as when the concentration of one or more metabolic products starts to plateau (or even drop) after a period of monotonically increasing.
- In some instances, microorganisms may be provided in the mixtures themselves, and/or in separate solutions introduced to the geologic formation. The microorganisms may be provided to areas of the geologic formation (such as the carbonaceous material) that show little or no biological activity. They may also be provided to increase the microorganism population in areas where microorganisms are already present (e.g., where there is already a native microorganism population.) The added microorganisms may be selected to work in concert with the agent/nutrient mixture supplied to the formation.
- Compounds used in the methods described may act as nutrients, activation agents, initiators, or catalysts for increasing the production of biogenic gases including hydrogen and methane. As nutrients, the microorganisms may consume the compounds allowing the microorganism populations to grow more rapidly than without the compounds. As activation agents, the compounds may lower an activation barrier, open a metabolic pathway, modify a carbonaceous material, change the reaction environment, and may or may not be rapidly consumed as a nutrient.
- When the compound is acting primarily like a nutrient, consumption of the nutrient by the microorganisms may increase the production of biogenic gas by a stoichiometrically proportional amount to that of the compound used. Alternatively, when a compound is acting primarily as an activation agent, the increased amount of biogenic gas may be much larger than the amount of the compound added. Thus, in such a scenario, introducing small quantities of the compound may produce much more than stoichiometric quantities of the biogenic gases. When the compound acts as an activation agent, the compound may or may not act as a catalyst, and may be fully, partially, or not consumed while increasing the production of biogenic gas.
- Turning now to
FIG. 1 , aflowchart illustrating methods 100 of dispersing a mixture within a geologic formation according to embodiments of the invention is shown. Themethods 100 may include accessing thegeologic formation 110. The geologic formation may be a previously explored, carbonaceous material-containing subterranean formation, such as a coal mine, oil field, natural gas deposit, carbonaceous shale deposit, etc. In many instances, access to the formation may involve utilizing previously mined or drilled access points to the formation. For unexplored formations, accessing the formation may involve digging or drilling through a surface layer to access the underlying site. The geologic formations may include native carbonaceous materials that were formed in the formation through natural processes instead of being supplied to the formation through a human-directed process (e.g., dumping or pumping the carbonaceous material into the formation). - Accessing the
geologic formation 110 may also include accessing microorganisms present in the formation. These microorganisms may include methanogenic microorganisms that convert adjacent carbonaceous material into hydrogen (H2), methane (CH4), and/or other metabolic products that have hydrogen-to-carbon ratios higher than the starting carbonaceous material. The microorganisms may also include species of methanogenesis inhibitors that slow or inhibit methanogenic metabolic processes by consuming methanogenic precursors and/or producing compounds that inhibit methanogenesis. - The
method 100 may include abiological analysis 112 of the microorganisms. This may include a quantitative analysis of the population size determined by direct cell counting techniques, including the use of microscopy, DNA quantification, phospholipid fatty acid analysis, quantitative PCR, protein analysis, etc. The identification of the genera and/or species of one or more members of the microorganism consortium by genetic analysis may also be conducted. For example, an analysis of the DNA of the microorganisms may be done where the DNA is optionally cloned into a vector and suitable host cell to amplify the amount of DNA to facilitate detection. In some embodiments, the detecting is of all or part of ribosomal DNA (rDNA), of one or more microorganisms. Alternatively, all or part of another DNA sequence unique to a microorganism may be detected. Detection may be by use of any appropriate means known to the skilled person. Non-limiting examples include restriction fragment length polymorphism (RFLP) or terminal restriction fragment length polymorphism (TRFLP); polymerase chain reaction (PCR); DNA-DNA hybridization, such as with a probe, Southern analysis, or the use of an array, microchip, bead based array, or the like; denaturing gradient gel electrophoresis (DGGE); or DNA sequencing, including sequencing of cDNA prepared from RNA as non-limiting examples. The identification of putative metabolic functions that are encoded in the DNA of individual organisms may also be conducted by employing Single Cell Whole Genome Sequencing (SC-WGS). The isolation of hundreds of individual cells in a sample from the geologic formation may be achieved. Hundreds of individual cells may be isolated using fluorescence-activated cell sorting to separate and deposit each cell into its own well in a microtiter plate. The deposited cell may be lysed, its entire genome amplified then screened for identity using SSU rRNA census sequencing techniques and appropriate amplified genomes selected for genome sequencing. Gene annotation of SC-WGA samples can be done using the Integrated Microbial Genomes (IMG) and RAST databases to provide a comprehensive comparative analysis of putative gene function and uncover the dominant metabolic and degradation pathways for the most abundant and active bacteria within the samples. Additional details of the biological analysis of the microorganisms is described in co-assigned U.S. patent application Ser. No. 11/099,879, filed Apr. 5, 2005, the entire contents of which is herein incorporated by reference for all purposes. By determining characteristics of the microorganisms and dominant metabolic functions, activation agents and nutrients may be provided that target particular microorganisms or metabolic pathways in order to stimulate or favor metabolism of the carbonaceous material to make particular metabolic products or biogenic gases. - The
method 100 may also include environmental analysis of the formation environment. For example, extracted geologic formation samples such as water, rock, and sediment bearing the carbonaceous material may be analyzed using spectrophotometry, NMR, HPLC, gas chromatography, mass spectrometry, voltammetry, and other chemical instrumentation. The tests may be used to determine the presence and relative concentrations of elements like dissolved carbon, phosphorous, nitrogen, sulfur, magnesium, manganese, iron, calcium, zinc, tungsten, cobalt, and molybdenum, among other elements. The analysis may also be used to measure quantities of polyatomic ions such as PO2 3−, PO3 3−, and PO4 3−, NH4 +, NO2 −, NO3 −, and SO4 2−, among other ions. The quantities of vitamins, and other nutrients may also be determined. An analysis of the pH, salinity, oxidation potential (Eh), and other chemical characteristics of the formation environment may also be performed. Additional details of chemical analyses that may be performed are described in co-assigned PCT Application No. PCT/US2005/015259, filed May 3, 2005; and U.S. Pat. No. 7,426,960, filed Jan. 30, 2006, of which the entire contents of both applications are herein incorporated by reference for all purposes. - Once access to the geologic formation is available, a mixture may be provided 115 to stimulate the production of biogenic gas (e.g., methanogenesis). The mixture may take the form of an emulsion that incorporates one or more activation agents and/or nutrients that are provided to the geologic formation. Techniques for providing the
mixture 115 may include direct injection processes that pump and/or pour the mixture into the formation environment. - The mixture may be non-homogeneous or homogeneous, and may include multiple phases, including solid and liquid phases, and two or more liquid phases. Exemplary mixtures having two or more liquid phases may include emulsions that have one or more dispersed phases surrounded by a continuous phase. The lack of miscibility that causes the separate liquid phases may be due to different polarities of the liquids. For example, a dispersed liquid phase may be non-polar while the continuous phase is polar. Conversely the dispersed phase may have a polar liquid while the continuous phase is made from a non-polar liquid.
- Exemplary emulsions may include oil-in-water (O/W) emulsions that have a non-polar dispersed phase incorporated into a continuous phase that include polar water molecules. They may also include water-in-oil (W/O) emulsions where droplets of polar water or an aqueous solution are dispersed in a non-polar hydrocarbon-containing continuous phase. The emulsions may be classified as microemulsions and/or nanoemulsions if the size of the dispersed-phase droplets are the requisite size. The emulsion may be a single emulsion containing two-phases, such as O/W, or may alternatively, in some embodiments, be a multiple emulsion including an emulsion contained in a separate continuous phase, such as W/O/W for example. The mixture may further contain surfactants and/or emulisifiers that slow or prevent the dispersed phases from coagulating and/or forming a separate layer of the mixture.
- The activation agents and/or nutrients may be dissolved in the one or more dispersed phases, the continuous phase, or both. For example, the agents/nutrients may be soluble in a polar solvent such as water (i.e., an aqueous phase), or a non-polar solvent such as found in an “oil” phase. Depending on whether the emulsion is (1) oil-in-water or (2) water-in-oil, the agents/nutrients that are soluble in the aqueous phase would be found in the continuous phase of (1), and dispersed phase of (2), respectively. Agents and nutrients that are at least partially soluble in both polar and non-polar solvents may be found in both the dispersed and continuous phases of the emulsions. Examples may further include agents and nutrients that are partially dissolved in one or more of the liquid phases of the emulsion, and that also have a solid phase component suspended and/or precipitated from the liquid phases.
- In exemplary emulsions that include polar aqueous phases and a non-polar (e.g., “oil”) phase, a non-polar “oil” phase may include compounds having at least a non-polar, hydrophobic/lipophilic moiety such as a long chain hydrocarbon. Examples of these non-polar compounds may include mineral oils, essential oils, and organic oils, among other types of oils. They may further include lipids and fatty acids that include non-polar, hydrophobic hydrocarbon tails. Exemplary fatty acids may include naturally occurring, saturated and/or unsaturated fatty acids such as myristoleic acid, palmitoleic acid, sapienic acid, oleic acid, linoleic acid, α-linolenic acid, arachidonic acid, eicosapentaenoic acid, erucic acid, docosahexaenoic acid, ricinoleic acid, etc., among other fatty acids having one or more double-bonds between carbon atoms in the hydrocarbon chains, and including configurations with hydrogen atoms being located on the same or opposite sides of the double bond, such as with cis- or trans-configurations of the acids. Exemplary saturated fatty acids may include lauric acid, myristic acid, palmitic acid, stearic acid, arachidic acid, behenic acid, lignoceric acid, cerotic acid, etc., among other fatty acids that have no double bonds and are saturated with hydrogen atoms. Some of the possible combinations of acids that may be used in the non-polar phase may be from naturally occurring fats and oils, and may include animal fats including lard, duck fat, butter, as well as vegetable fats including coconut oil, palm oil, cottonseed oil, wheat germ oil, soya or soybean oil, olive oil, corn oil, sunflower oil, safflower oil, hemp oil, canola oil, among others. Other possible combinations may include engineered formulations that have been shown to remain dispersed in oil-in-water emulsions for particular periods of time without creaming or separation of the dispersed non-polar phase.
- Polar phase compounds may include water, or aqueous solutions incorporating salts, sugars, proteins, amino acids, chlorogenic acids, protein hydrolyzates, various cell extracts such as yeast extract, algal extracts, or other compounds including volatile fatty acids, or acids such as acetic acid, propionate, butyrate, oxyphosphorous acids, etc. Additional exemplary polar phase compounds may include formamide, dimethyl sulfoxide, and ferulic acid among others. The included algal extracts may include Chlamydomonaas spp., or Spirulina spp., for example.
- A compound that acts as a nutrient or activation agent may be contained within one or more phases of the mixture that is provided 115 to the geologic formation. The compound may stimulate the microorganisms to metabolize carbonaceous material in the formation into biogenic gas, such as methane, or into intermediate metabolites that may further be metabolized into biogenic gases. The activation agent may contain various compounds for stimulating microorganisms in the formation environment. Activation agents provided may include phosphorous compounds, acetate compounds, or other nutrients for the microorganisms.
- The phosphorus compounds may include phosphorus compounds (e.g., POx compounds were x is 2, 3 or 4), such as sodium phosphate (Na3PO4) and potassium phosphate (K3PO4), as well as monobasic and dibasic derivatives of these salts (e.g., KH2PO4, K2HPO4, NaH2PO4, Na2HPO4, etc.). They may also include phosphorus oxyacids and/or salts of phosphorus oxyacids. For example, the phosphorus compounds may include H3PO4, H3PO3, and H3PO2 phosphorus oxyacids, as well as dibasic sodium phosphate and dibasic potassium phosphate salts. The phosphorus compounds may also include alkyl phosphate compounds (e.g., a trialkyl phosphate such as triethyl phosphate), and tripoly phosphates. The phosphorus compounds may further include condensed forms of phosphoric acid, including tripolyphosphoric acid, and pyrophosphoric acid, among others. They may also include the salts of condensed phosphoric acids, including alkali metal salts of tripolyphosphate (e.g., potassium or sodium tripolyphosphate), among other salts, and may also include oxides of phosphorus (e.g., phosphorus trioxide, pentoxide, etc.), among other compounds.
- Examples of the acetate compounds may include acetic acid, and/or an acetic acid salt (e.g., an alkali metal salt of acetic acid, an alkali earth metal salt of acetic acid, sodium acetate, potassium acetate, etc.), among other acetate compounds. Other activation agents that may be provided include nutrients such as yeasts and yeast extracts, and may include digests and extracts of commercially available brewers and bakers yeasts. Other activation agents may include nutrients including carboxylate compounds, proteins, hydrogen release compounds, minerals, metal salts, and/or vitamins, among other components. Catalysts may also be provided in the emulsions to activate the microorganisms or particular metabolic pathways. Still other compounds that may be included in the emulsion include cyclic and aromatic compounds that may include either or both of an ether linked group and an ester linked group, and may include vanillin and syringic acid, among other compounds and acids with a phenol group or other aryl group, and functional groups that may include, in some embodiments, hydroxyl groups, carboxylates, aldehydes, ethers, esters, etc., among others.
- The emulsion may be provided 115 in several separate applications over time as opposed to a single application. These separate applications may provide multiple stages of activation or stimulation over a period of time that may be monitored either through the rate of production of biogenic gas, or alternatively through in situ measurements of the concentrations of the activation agent or a metabolic byproduct produced during the conversion of the carbonaceous material. Additionally, these introductions may be made to the formation over the course of the activation period to maintain a certain concentration level or range of the activation agent in the formation.
- Once the mixture has been formed and provided, it may be dispersed through the
geologic formation 120. The dispersion of the mixture may be done using a variety of techniques, such as high-pressure pumping that forces the mixture to permeate the formation environment. The dispersion pattern of a mixture's agents and/or nutrients with respect to the formation and the carbonaceous material may be based at least in part on how the liquid phases are emulsified and how the agents/nutrients are dissolved in the liquid phases. For example, in a mixture that is an oil-in-water emulsion having the agent/nutrient dissolved in the non-polar, dispersed “oil” phase, the agent/nutrient may be transported further by the aqueous continuous phase across the carbonaceous material before being absorbed. Moreover, when the same emulsion encounters native formation water in contact with the carbonaceous material, the distribution of the non-polar dispersed phase may be more localized along the boundary of the formation water and carbonaceous material. It may also distribute the dispersed phase over a larger area of the carbonaceous material at the water-material boundary. In this example, more agent/nutrient may be supplied to the carbonaceous material by localizing the non-polar dispersed phase close to the surface of the material instead of diluting it throughout the adjacent formation water. - As the mixture is introduced to the formation, it may make direct contact with carbonaceous material and/or indirect contact by first being dispersed in the formation before reaching the carbonaceous material. The microorganisms may be found in a variety of locations in the geologic formation, including the carbonaceous material and/or the formation water, among other locations. The mixture may be dispersed within the
geologic formation 120 in order to reach the microorganisms that may then be stimulated by the activation agent. The microorganisms may access the activation agent directly, or in some embodiments, may consume the non-polar dispersed phase to access the activation agent. In consuming or metabolizing the non-polar dispersed phase, the microorganisms may be provided with an additional nutrient source in addition to the activation agent. Metabolizing the non-polar dispersed phase may produce an acetate compound that may be utilized by the same or different microorganisms within the formation environment as an additional nutrient source to stimulate the microorganisms. Metabolizing the non-polar dispersed phase may also produce hydrocarbons that are similar in nature and structure to the carbonaceous material being converted by the microorganisms, and may produce metabolic products including biogenic gases, among other products. -
Method 100 may also include measuring the rate ofbiogenic gas production 125. For the biogenic gas products, the partial pressure of the product in the formation may be measured. Measurements may also be made before providing the activation agent, and a comparison of the product concentration before and after the activation agent may also be made. The biogenic gas products may also be recovered from the formation environment, or maintained within the formation environment at a concentration range that has been found to stimulate the microorganisms to generate more of the biogenic gas product. Based on the rate of biogenic gas production, more or less activation agents or nutrients may be added to the geologic formation in order to maximize biogenic gas output. -
FIG. 2A shows a flowchart illustrating amethod 200 of producing a multiple emulsion incorporating one or more stimulating compounds according to embodiments of the invention. Themethod 200 may include providing both a polar aqueous fluid and anon-polar fluid 210. The non-polar fluid may be any of the previously mentioned oils or fatty acids, and may additionally be a fluid at least partially immiscible in water. The added compound may be combined with a first portion of thepolar fluid 212. The compound may be dissolved, mixed, suspended, or otherwise contacted with the fluid. Themethod 200 may also include adding an oil-soluble surface active agent (“surfactant”), and/or emulsifier to the non-polar fluid. The surfactant may be chosen based on its hydrophilic-lipophilic balance (“HLB”) number, which is an indication of the hydro- or lipophilicity of the surfactant. A higher HLB value describes a compound that is more hydrophilic, while a lower HLB value describes a compound that is more lipophilic. The surfactant used in the emulsion may have a lower HLB value, thus being more lipophilic, and exemplary surfactants may have HLB values below or about 10, below or about 8, below or about 6, below or about 4, or below or about 2. - Once the surfactant has been properly combined with the non-polar fluid, the
method 200 may include producing a primary emulsion by adding the portion of the polar fluid containing the nutrient to the non-polar fluid with thesurfactant 214. The emulsion may be produced by agitating the combined fluids in a blender, magnetic mixer, or another device that applies relatively high shear to the emulsion components. The emulsion may be further stabilized by processing the emulsion in a colloid mill or homogenizer, or a device that applies a very high level of hydraulic shear to the emulsion. This produced emulsion may have macro, micro, or nano-sized particles depending on the requirements of the particular application. The emulsion may also be produced in a device that raises the temperature of the components above room temperature during the processing. Raising the temperature of the emulsion may reduce the viscosity of the dispersed phase which may facilitate emulsification of the components. The temperature of the components may be raised during the processing to above or about 25° C., above or about 50° C., above or about 70° C., or above or about 90° C. - After the primary emulsion has been produced and stabilized, the
method 200 may further include making a multiple emulsion. A second portion of a polar fluid, which may include a surfactant and/or emulsifier, may be used as a continuous phase in the multiple emulsion. The surfactant may have a higher HLB value, making it more hydrophilic. For example, the surfactant may have an HLB value above or about 8, above or about 10, above or about 13, above or about 15, or above or about 18. - The
method 200 may also include adding the primary emulsion to the second portion of the polar fluid with the surfactant to produce amultiple emulsion 216. The multiple emulsion may be produced by agitating the combined fluids using a relatively low shear device to avoid separating the primary emulsion. For example, a blender or magnetic mixer may be utilized to produce both the primary emulsion and the secondary emulsion. The speed used for the secondary emulsion may be lower than the speed used for the primary emulsion, and may be, for example, 50% or less of the speed used in the primary emulsion. The degree of shear used in the preparation of the primary and secondary emulsion may vary depending on the polar and non-polar fluids and surfactants used, as well as the types of encapsulated compounds. A high-shear device may be used to produce the primary emulsion, such as a high-pressure homogenizer. A low-shear device may be used to create the secondary emulsion, such as a combined orifice device, or a device utilizing a membrane emulsification technique. Regardless of the devices used for preparing the emulsions, the osmotic gradient between the two portions of the aqueous fluids may be maintained at a low-enough threshold such that the encapsulated aqueous portion does not pass through the non-polar fluid phase into the continuous aqueous phase. The osmotic pressure between the two aqueous phases may be adjusted by changing the concentrations of the fluids, which may be done, for example, with the addition of the surfactants, or alternatively by adding various salts, sugars, sugar esters, and/or other compounds. - The surfactants used in the emulsions may be selected based on the compounds provided to the carbonaceous material. Surfactants used may include cationic surfactants, such as benzalkonium chloride; anionic surfactants, such as alkali or amine soaps, and detergents; and/or Zwitterionic surfactants, such as CHAPS or sultaines, amino and imino acids, or phosphates such as lecithin.
- The emulsions may also include nonionic emulsifying agents. Nonionic emulsifiers that may be used in either of the primary emulsion or the secondary emulsion may include, for example, polymeric and non-polymeric compounds, as well as fatty alcohols, such as cetyl alcohol, stearyl alcohol, cetostearyl alcohol, and oleyl alcohol, among others. Other examples of nonionic surfactants that may be used in the formation of the emulsions include glyceryl monostearate, polyoxyethylene monooleate or monostearate or monolaurate, and other polyoxyethylene glycol alkyl ethers (PEG-400 emulsifiers), polyoxypropylene glycol alkyl ethers, glucoside alkyl ethers, potassium oleate, sodium lauryl sulfate, and sodium oleate, among others. Still other examples of nonionic emulsifiers that may be used in the formation of the emulsions may include sorbitan alkyl esters (Spans), including sorbitan monolaurate (Span 20), sorbitan monopalmitate (Span 40), sorbitan monostearate (Span 60), sorbitan tristearate (Span 65), sorbitan monooleate (Span 80), sorbitan sesquioleate (Span 83), and sorbitan trioleate (Span 85), among others. Other examples of nonionic surfactants that may be used in the formation of the emulsions may include triethanolamine oleate, polyoxyethylene derivatives of sorbitan alkyl esters (Tweens), including polyoxyethylene sorbitan monolaurate (Tween 20), polyoxyethylene sorbitan monolaurate (Tween 21), polyoxyethylene sorbitan monopalmitate (Tween 40), polyoxyethylene sorbitan monostearate (Tween 61), polyoxyethylene sorbitan tristearate (Tween 65), polyoxyethylene sorbitan monooleate (Tween 80), polyoxyethylene sorbitan monooleate (Tween 81), and polyoxyethylene sorbitan trioleate (Tween 85), among others.
- The emulsions may also incorporate natural emulsifying agents, and/or hydrocolloids. For example, the emulsions may include vegetable derivatives, such as acacia, tragacanth, agar, pectin, carrageenan, lecithin, and others; or animal derivatives, such as gelatin, lanolin, and cholesterol, among others. Semi-synthetic agents such as methylcellulose, and carboxymethylcellulose, and fully synthetic agents such as Carbopols may also be used.
- Solid particle emulsifiers may be used to form a particulate layer around the dispersed phase. These agents may include dispersed particles of bentonite, veegum, hectorite, magnesium hydroxide, magnesium trisilicate, and aluminum hydroxide, among others.
- The
method 200 may also include transporting the emulsion to thegeologic formation 218. The transporting may be done in such a way as to maintain the stabilized emulsion, such as by utilizing transportation equipment and vehicles that will minimize changes in temperature or pressure on the emulsion. In some embodiments, the emulsion may be made at the site of the geologic formation where the compound may be injected, such that transportation may not be a concern. Where the emulsion must be transported over long distances, additional surfactants, mixing transportation vessels, or different amounts of the surfactants used in the preparation of the emulsion may be utilized to produce a stable emulsion. - The emulsion may be injected into the
geologic formation 220 to contact the carbonaceous material with the encapsulated compound. The injection may include a pumping mechanism to force the emulsion into the geologic formation, and disperse the emulsion over a greater area of the formation. Alternatively, the emulsion may be poured into the formation and allowed to remain at the site of the injection. Depending on the relative amounts of the polar and non-polar fluids used in the emulsion, the non-polar fluid may allow the activation agent to disperse over a larger area because the non-polar fluid may not be absorbed into the carbonaceous material, formation water, or the formation itself. - As shown in
FIG. 2B , amethod 230 is described in which a paused or staged dispersion may be created from the ways in which the nutrients or other compounds are incorporated within the emulsion. Themethod 230 may provide a polar and anon-polar fluid 240 as previously described. The activation agent or nutrient compound may be disposed 242 within the polar fluid. Portions of the polar fluid containing the compound may be used both within the non-polar phase of the mixture in the first emulsion produced 244, as well as for the continuous phase used for themultiple emulsion 246. This emulsion may then be transported to thegeologic formation 248. Microorganisms may access this portion of the activation agent directly when the mixture has been injected 250 into the carbonaceous material. The non-polar phase may be consumed by the microorganisms as a nutrient source, and the consumption of the non-polar phase may release the portion of the activation agent or nutrient contained therein. By providing the portion of the activation agent as encapsulated within the non-polar dispersed phase, the release of this portion of the activation agent may occur at a time later than that at which the portion of the activation agent disposed within the continuous aqueous phase was consumed. - A paused or delayed dispersion may allow greater amounts of nutrients or activation agents to be delivered at lower concentrations over longer periods of time to the carbonaceous material than could otherwise be provided. Adding large amounts of a specific compound to the geologic formation in one dose may in some circumstances create deleterious effects including the death or deactivation of microorganisms. However, by creating multiple emulsions that stage the release of smaller portions of a compound at a time, an overall greater amount of the compound may be used in a single injection. The larger amounts may allow fewer applications of the activation agents to the geologic formation, which may provide for improved consistency of the applications to create a more controlled stimulation of the microorganisms within the formation.
- Paused or staged dispersion may allow time for the microorganisms to grow or activate before the additional portion of the activation agent may be released. For example, a greater portion of the activation agent may be contained in the non-polar dispersed phase than in the aqueous continuous phase. Thus, after the microorganisms are activated by the portion of the nutrient contained in the continuous phase, they may be provided with a period of time in which they may grow as a consortium. The non-polar dispersed phase may be consumed as a nutrient by the microorganisms, the consumption of which may release equivalent or greater portions of the activation agent. This second dosing with larger amounts of activation agent may be more effectively utilized by a larger and/or more robust microorganism population that has been given time to grow and acclimate to metabolizing coal carbon to new biogenic methane gas.
- Alternatively, a greater portion of the activation agent may be contained in the polar continuous phase of the emulsion, and a lesser amount within the non-polar dispersed phase. Thus, after the greater portion of the activation agent has been utilized to activate the microorganisms, and the microorganisms have penetrated the non-polar dispersed phase, either by its consumption or by the breakdown of the emulsion due to separation, the lesser amount of the activation agent may be utilized to maintain the activation of the microorganisms during their metabolizing of the carbonaceous material. A potential cost savings may be realized by providing the correct proportions of nutrients or activation agents based on the characteristics or dynamics of the microorganisms. Additionally, fewer follow-on introductions of nutrients or activation agents may be required.
- As shown in
FIG. 2C , amethod 260 is described in which a multiple emulsion may be created that contains both an activation agent and a nutrient. By providing both an activation agent as well as a nutrient source to a microorganism population, the microorganisms may be capable of increased growth and ability to convert carbonaceous material due to a compound effect provided by the combination of elements. Themethod 260 may include the step of providing a polar andnon-polar fluid 270, substantially as described previously. A nutrient may be combined 274 within a first portion of a polar fluid, and an activation agent may be combined 276 with a second portion of the polar fluid. An emulsion may be produced 278 within the non-polar fluid with the portion of the polar fluid containing the nutrient. The portion of the polar fluid containing the activation agent may then be used as the continuous phase of a multiple water-in-oil-in-water emulsion 280 containing the emulsion produced with the nutrient as the dispersed phase. The emulsion may then be transported to thegeologic formation 282, and when introduced to acarbonaceous material 284, the activation agent located in the continuous aqueous phase may stimulate the microorganisms to metabolize the carbonaceous material. Then, at a later point than the stimulation by the activation agent, the microorganisms may either consume as a nutrient source, or surpass due to separation, the non-polar dispersed phase, thereby releasing the nutrient contained therein for consumption by the microorganisms. -
FIGS. 3A-C show methods of forming a single emulsion including a polar fluid and a non-polar fluid.FIG. 3A shows amethod 300 in which a polar and non-polar fluid may be provided 310, and a compound may be incorporated into thepolar fluid 312. The polar fluid may be dispersed within the non-polar fluid producing anemulsion 314. This water-in-oil type emulsion may then be directly injected into the geologic formation to contact the carbonaceous material. In some embodiments, the non-polar continuous phase may be consumed or metabolized by the microorganisms as a nutrient source. The metabolic products created by the breakdown of the non-polar continuous phase may include acetate compounds, as well as other hydrocarbons including biogenic gases, among other compounds. Metabolizing the non-polar continuous phase may release the nutrient or activation agent contained in the polar aqueous phase, which may be further consumed to stimulate the microorganisms to convert the carbonaceous material into biogenic gas. Alternatively, as shown inFIG. 3B , amethod 320 is described that also may provide a polar andnon-polar fluid 330. The compound may be combined with thenon-polar fluid 332, which may then be encapsulated in a polaraqueous fluid 334 and injected into a geologic formation as described previously. In another alternative, a compound may be disposed in at least one or both of the emulsion phases, which may allow a staged or paused release of the compound to the microorganisms within the formation as discussed above. - As shown in
FIG. 3C , amethod 340 may provide a polar andnon-polar fluid 350, and may include suspending microorganisms within thepolar fluid 352. This polar fluid may then be encapsulated within a non-polar fluid to produce anemulsion 354. This emulsion may be combined with a polar fluid to produce a multiple emulsion, or alternatively, may be injected directly into the geologic formation. In another alternative, the polar aqueous continuous phase of the multiple emulsion may additionally contain other nutrients or activation agents to be consumed by the encapsulated microorganisms or by microorganisms native to the formation. - The geologic formation environment may be anaerobic, and thus, the production and transportation of the emulsions may occur under anaerobic conditions. For example, the encapsulated microorganisms may be contained in an anaerobic aqueous fluid used in the primary emulsion. Anaerobic fluid is characterized as having little or no dissolved oxygen, in general no more than 4 mg/L, preferably less than 2 mg/L, and most preferably less than 0.1 mg/L, as measured at 20° C. and 760 mmHg barometric pressure. The microorganisms may be from the same geologic formation to which they will be injected, and are being reinjected in order to be dispersed to alternative portions of the geologic formation, or to a broader area of the geologic formation. Alternatively, the microorganisms may be from a different geologic formation and are being transported to the geologic formation containing the carbonaceous material sought to be converted into biogenic gas. In these or other cases, the microorganisms may have been modified, for example genetically, prior to their being injected or reinjected into the geologic formation. In order to maintain an anaerobic environment for the microorganisms, equipment and vehicles that are oxygen impermeable may be used, otherwise the microorganisms may be damaged in the process.
-
FIG. 4 shows a block diagram for selected components of asystem 400 for creating a multiple emulsion according to embodiments of the invention. Low-HLB surfactant 411 may be incorporated intonon-polar fluid 412 that may be added to mixingvessel 410.Activation agent 403 may be incorporated into polaraqueous fluid 407, that may then be incorporated intonon-polar fluid 412 in mixingvessel 410 to create a primary emulsion. Mixingvessel 410 may be, among other equipment, a high-shear mixer, blender, or mortar that may create macro, micro, and/or nanoemulsions. The primary emulsion may be further stabilized and refined in high-shear device 420, such as a high-pressure homogenizer, or a colloid mill through which the primary emulsion may be passed. Either or both of the mixingvessel 410 and the high-shear device 420 may be heated to a temperature to facilitate the production of the primary emulsion. Alternative embodiments may include using only one of the mixingvessel 410 and high-shear device 420. - High-
HLB surfactant 421 may be incorporated into polaraqueous fluid 422 in low-shear device 430. The primary emulsion may be added to the polaraqueous fluid 422 in low-shear device 430 to create amultiple emulsion 440. Low-shear device 430 may include a blade-type or magnetic mixer or blender that operates at a speed lower than mixingvessel 410. Alternatively, low-shear device 430 may include a membrane through which the primary emulsion may be passed or pressed. Continuous phase, polaraqueous fluid 422 may be flowed across the membrane of the low-shear device separating droplets of the primary emulsion and creating a secondary,multiple emulsion 440. -
FIG. 5 shows selected components of asystem 500 for supplying an encapsulated nutrient to a formation environment according to embodiments of the present invention.Multiple emulsion 440 may be produced atgeologic formation 510. Themultiple emulsion 440 may be transported to thegeologic formation 510 or created at the site of thegeologic formation 510.Multiple emulsion 440 may be injected intogeologic formation 510 via pipes orwells 520 either previously located in or presently placed in an access point in thegeologic formation 510.Pumping mechanism 535 may also be utilized to inject the multiple emulsion into thegeologic formation 510 to disperse themultiple emulsion 440 over a greater area of the geologic formation. Additionally, pumping mechanisms may transport make up water to the well head that may gravity feed into thewell 520. A separate pumping mechanism may supply a steady stream rate ofemulsion 440 to the gravity feed water stream going into well/pipes 520. - Alternatively,
system 500 may include transferringmultiple emulsion 440 into avehicle 540 to be delivered to one or more locations at ageologic formation 510.Vehicle 540 may transport themultiple emulsion 440 to alternative geologic formations. When themultiple emulsion 440 is maintained under anaerobic conditions, oxygenimpermeable pipes 530 andvehicles 540 may be utilized to prevent the introduction of oxygen to theemulsion 440. - Prior to transportation in
vehicle 540, additional emulsifiers, surfactants, or stabilizers may be added to themultiple emulsion 440 in order to facilitate transporting the emulsion in its dispersed form, and to resist separation of the phases. Whenvehicle 540 arrives at the geologic formation at which themultiple emulsion 440 is to be introduced,multiple emulsion 440 may be added to mixingdevice 550 to more uniformly disperse any of the primary emulsion that has separated frommultiple emulsion 440. - Experiments were conducted to determine the stability of the emulsion in the presence and absence of coal particles. Into non-sterile micro-centrifuge tubes, emulsified soybean oil emulsion was pipetted in varying dilutions with water filtered by reverse osmosis to a final volume of 1000 mL. Two identical sets were made. The first set contained emulsified oil only, and was examined at several concentrations including 100%, 50%, 10%, and 1%. The second set was prepared by adding 0.5 g of <600 μm ground Anderson coal to the tubes along with the emulsified oil. After liquid additions, all tubes were tightly capped and blended by vortex mixer prior to incubation over night at room temperature. The samples were viewed under magnification using direct light/oil emersion microscopy. Distinct round emulsion micelles were observed at similar numbers in both the coal amended and unamended samples within the same dilution level. This was true at all dilution levels, and both sets of samples were then monitored at set periods of time to determine if and when separation occurred.
- Microcosms tests were performed using fresh coal bed methane water from the Powder River Basin with and without 0.5 grams of ground Anderson coal. Emulsified oil amendment was tested for its ability to activate methanogenic coal conversion and compared to various nutritional controls. A microscopic examination of samples from methanogenically active microcosms tests showed the presence of emulsified oil micelles at significantly reduced particle numbers at 150 days after test initiation. These results suggest that amendment materials contained within the emulsion micelles may still be available for long-term slow-release to microorganism consortiums. Additionally, the oil that may be included within the emulsion is also available to the microorganisms for conversion.
- Experiments conducted also compared methane production by microorganism consortiums being provided emulsified oil and various other amendments.
FIG. 6 is a chart detailing various amendment compounds and methane produced over time in a controlled lability experiment. In this experiment, no carbonaceous material, i.e. coal, was provided to the microorganisms during the course of the treatment. As shown, emulsion based amendments were provided in addition to standard amendments as well as slow-release lactate amendments. As can be identified, the two amendments of emulsified oil produced significantly more methane at faster rates over the experimental period than did any other amendment. Without being limited to any specific theory, the inventors believe that fatty acids of the emulsified oils undergo metabolism through beta oxidation by which acetate units are cleaved from the long chain hydrocarbons. The derived acetate can be utilized as an activation agent to stimulate the microorganisms in the production of methane. Additionally, the emulsions that also contained a phosphate and yeast extract amendment produced substantially more methane over the course of the procedure. This may provide both the activation potential of the acetate source as well as the nutrients that can be utilized over a longer period of time due to the encapsulation. - A similar set of experiments was conducted to those illustrated in
FIG. 6 that compared methane production utilizing various amendment compounds over time that included a carbonaceous substrate. The results are included inFIG. 7 . Similar to the results reported for the lability experiment, the methane production was significantly higher in the samples receiving emulsified oil based amendments. Similar inferences can be made from the results of the experiments containing carbonaceous material as with the experiments without carbonaceous materials. The difference between the two figures illustrates the carbonaceous material metabolized by the microorganisms inFIG. 7 that may result in the increased methane production. -
FIG. 8 illustrates acetate turnover over time in an additional lability experiment performed with similar amendment samples. As shown the emulsified oil based samples produced acetate that was initially accumulated in the system. However, instead of producing a build-up of metabolic product, the released acetate was consumed over a course of time for the experiment. Thus, the microorganism consortium may consume the released acetate after metabolizing the emulsified oil. Additionally, microorganisms that may better utilize the acetate, such as acetoclastic methanogens, may flourish as a result of the acetate available and grow to a more significant portion of the consortium. As illustrated, the emulsified oil including the phosphate and yeast extract produced a greater initial amount of acetate in the system followed by a faster utilization of the produced acetate by the consortium. - An additional experiment including carbonaceous material was conducted with similar amendment samples to determine the amount and degree of acetate turnover as shown in
FIG. 9 . As with the methane production experiments, the inclusion of carbonaceous material increased the amount of acetate produced in the initial period of accumulation. Also, the emulsion including phosphate and yeast extract produced a faster turnover of the accumulated acetate than did the sample including the emulsified oil alone. This effect may be a result of the nutrient benefit provided by the phosphate and yeast amendment. - Considering
FIGS. 7 and 9 together indicates that the acetate accumulated initially is consumed, followed by enhanced production of methane. For example,FIG. 9 illustrates that after an initial accumulation of acetate during the first 50 days, the acetate is consumed. This consumption correlates to the increase of methane production beginning at roughlyday 50 ofFIG. 7 . This phenomenon may be a result of acetoclastic methanogenic organisms increasing in population followed by a period of activity during which the organisms consume the acetate thereby producing methane. The available acetate may activate the growth of the consortium, and release of the phosphate and yeast extract when the emulsified oil is metabolized may provide nutrients for stimulating methane production. As such, the encapsulated nutrients may be utilized in an improved fashion as compared to a bolus of nutrients as they may be consumed over a longer period of time due to the encapsulation. Thus, less of the nutrients may be needed due to a more efficient utilization by the consortium. - An additional experiment creates additional emulsion products useful according to the present technology. Polyglycerol polyricinoleate (“PGPR”) was blended into soybean oil at 8% by weight (8 g PGPR:100 g Soybean oil) until the solution appeared visibly homogenized in a first solution. Xanthan gum was blended into a solution of yeast extract, acetate, and phosphate at 0.2% by weight to make a second solution. Equal parts of the first and second solutions were blended in bursts at high speed (>5000 RPM) until a viscous product was formed as a third product.
- An additional solution of yeast extract, acetate, and phosphate was added to a fresh vessel and stirred at room temperature. To the stirring solution was added 0.5% by weight of a 1:1 ratio of Tween 20 and Tween 80. The solution was stirred for several minutes at low speed (<500 RPM) until bubbles developed. The third product was slowly added to the solution while mixing continued. The final product was blended for several minutes more at low to moderate speed (<1000 RPM) to avoid incorporating air into the mixture.
-
FIG. 10 shows a microscope image of the formed emulsions at 400× magnification directly after production. The graph shows formed emulsion droplets at 5 μm and 20 μm respectively as indicated, and shows the incorporation of yeast extract, acetate, and phosphate solution within the oil solution.FIG. 11 shows the same solution after 24 hours has elapsed showing that the yeast extract, acetate, and phosphate droplets have coalesced into cores of agent within the oil droplets.FIG. 12 shows additional views of emulsion species located within certain mixtures of the present technology.Droplets 1210 show large cores of the extract solution that are coalesced within the oil droplets.Droplets 1220 show water-in-oil nanodroplets that are dispersed throughout the mixture.Droplets 1230 show WOW particles produced in the mixture. - It will be understood by one of ordinary skill in the art that the embodiments may be practiced differently in different environments. For example, one environment may include wireless control of processes or machinery from a remote terminal that can provide automatic instruction, while another environment may include no such control and is operated locally at the site of the process based on then current operation conditions.
- Additionally, it will be understood by one of ordinary skill in the art that the embodiments may be practiced without these specific details. For example, machinery, systems, networks, processes, and other elements in the invention may be shown as components in block diagram form in order not to obscure the embodiments in unnecessary detail. In other instances, well-known processes, structures, and techniques may be shown without unnecessary detail in order to avoid obscuring the embodiments.
- Also, it is noted that individual embodiments may be described as a process which is depicted as a flowchart, a flow diagram, a data flow diagram, a structure diagram, or a block diagram. Although a flowchart may describe the operations as a sequential process, many of the operations can be performed in parallel or concurrently. In addition, the order of the operations may be rearranged. A process may be terminated when its operations are completed, but could have additional steps not discussed or included in a figure. Furthermore, not all operations in any particularly described process may occur in all embodiments. A process may correspond to a method, a system, a procedure, etc.
- As used herein and in the appended claims, the singular forms “a”, “an”, and “the” include plural references unless the context clearly dictates otherwise. Thus, for example, reference to “a process” includes a plurality of such processes, and reference to “the nutrient” includes reference to one or more nutrients and equivalents thereof known to those skilled in the art, and so forth.
- Also, the words “comprise”, “comprising”, “include”, “including”, and “includes”, when used in this specification and in the following claims, are intended to specify the presence of stated features, integers, components, or steps, but they do not preclude the presence or addition of one or more other features, integers, components, steps, acts, or groups.
- The description and examples above are not intended to limit the scope, applicability, or configuration of the application to only what has been described. It should be understood that various changes may be made in the function and arrangement of elements without departing from the spirit and scope of the invention as set forth in the appended claims.
Claims (34)
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CA2868028A CA2868028A1 (en) | 2012-03-20 | 2013-03-20 | Dispersion of compounds for the stimulation of biogenic gas generation in deposits of carbonaceous material |
US13/847,756 US20140034297A1 (en) | 2012-03-20 | 2013-03-20 | Dispersion of compounds for the stimulation of biogenic gas generation in deposits of carbonaceous material |
AU2013235122A AU2013235122A1 (en) | 2012-03-20 | 2013-03-20 | Dispersion of compounds for the stimulation of biogenic gas generation in deposits of carbonaceous material |
PCT/US2013/033184 WO2013142619A1 (en) | 2012-03-20 | 2013-03-20 | Dispersion of compounds for the stimulation of biogenic gas generation in deposits of carbonaceous material |
ZA2014/07134A ZA201407134B (en) | 2012-03-20 | 2014-10-01 | Dispersion of compounds for the stimulation of biogenic gas generation in deposits of carbonaceous material |
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US13/847,756 US20140034297A1 (en) | 2012-03-20 | 2013-03-20 | Dispersion of compounds for the stimulation of biogenic gas generation in deposits of carbonaceous material |
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Cited By (3)
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US9518211B2 (en) * | 2013-01-25 | 2016-12-13 | Basf Se | Method for recovering oil |
CN108138036A (en) * | 2015-08-12 | 2018-06-08 | 联邦科学技术研究组织 | Methane phase |
US20190300912A1 (en) * | 2018-03-29 | 2019-10-03 | Transworld Technologies Inc. | Biologically enhanced oil recovery methods |
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US10465121B2 (en) | 2013-11-20 | 2019-11-05 | Nelson Mandela Metropolitan University | Processing carbonaceous materials |
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US20100190203A1 (en) * | 2006-04-05 | 2010-07-29 | Luca Technologies, Inc. | Chemical amendments for the stimulation of biogenic gas generation in deposits of carbonaceous material |
US20100248321A1 (en) * | 2009-03-27 | 2010-09-30 | Luca Technologies, Inc. | Surfactant amendments for the stimulation of biogenic gas generation in deposits of carbonaceous materials |
-
2013
- 2013-03-20 AU AU2013235122A patent/AU2013235122A1/en not_active Abandoned
- 2013-03-20 WO PCT/US2013/033184 patent/WO2013142619A1/en active Application Filing
- 2013-03-20 US US13/847,756 patent/US20140034297A1/en not_active Abandoned
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US20100190203A1 (en) * | 2006-04-05 | 2010-07-29 | Luca Technologies, Inc. | Chemical amendments for the stimulation of biogenic gas generation in deposits of carbonaceous material |
US20100248321A1 (en) * | 2009-03-27 | 2010-09-30 | Luca Technologies, Inc. | Surfactant amendments for the stimulation of biogenic gas generation in deposits of carbonaceous materials |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
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US9518211B2 (en) * | 2013-01-25 | 2016-12-13 | Basf Se | Method for recovering oil |
CN108138036A (en) * | 2015-08-12 | 2018-06-08 | 联邦科学技术研究组织 | Methane phase |
US20180237683A1 (en) * | 2015-08-12 | 2018-08-23 | Commonwealth Scientific And Industrial Research Organisation | Methanogenesis |
US10738234B2 (en) * | 2015-08-12 | 2020-08-11 | Commonwealth Scientific And Industrial Research Organisation | Methanogenesis |
US20190300912A1 (en) * | 2018-03-29 | 2019-10-03 | Transworld Technologies Inc. | Biologically enhanced oil recovery methods |
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ZA201407134B (en) | 2017-04-26 |
WO2013142619A1 (en) | 2013-09-26 |
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