US20130324550A1

US20130324550A1 - 1-(2-phenoxymethylheteroaryl) piperidine and piperazine compounds

Info

Publication number: US20130324550A1
Application number: US13/963,255
Authority: US
Inventors: Eric L. Stangeland; Lori Jean Patterson; Sheila Zipfel
Original assignee: Theravance Inc
Current assignee: Theravance Biopharma R&D IP LLC
Priority date: 2010-03-22
Filing date: 2013-08-09
Publication date: 2013-12-05
Also published as: WO2011119461A1; EP2550252B1; ES2543064T3; EP2550252A1; JP5769348B2; US8530663B2; US20110230495A1; JP2013522363A

Abstract

The invention relates to compounds of formula I:

where X, HAr, a, and R¹through R⁶are as defined in the specification, or a pharmaceutically acceptable salt thereof. The compounds of formula I are serotonin and norepinephrine reuptake inhibitors. The invention also relates to pharmaceutical compositions comprising such compounds; methods of using such compounds; and process and intermediates for preparing such compounds.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS

This application claims the benefit of U.S. Provisional Application No. 61/316,090, filed on Mar. 22, 2010; the entire disclosure of which is incorporated herein by reference.

BACKGROUND OF THE INVENTION

1. Field of the Invention
The present invention relates to 1-(2-phenoxymethylheteroaryl)piperidine and 1-(2-phenoxymethylheteroaryl)piperazine compounds having activity as serotonin (5-HT) and norepinephrine (NE) reuptake inhibitors. The invention also relates to pharmaceutical compositions comprising such compounds, processes and intermediates for preparing such compounds and methods of using such compounds to treat a pain disorder, such as neuropathic pain, and other ailments.
2. State of the Art
Pain is an unpleasant sensory and emotional experience associated with actual or potential tissue damage, or described in terms of such damage (International Association for the Study of Pain (IASP), Pain Terminology). Chronic pain persists beyond acute pain or beyond the expected time for an injury to heal (American Pain Society. “Pain Control in the Primary Care Setting.” 2006:15). Neuropathic pain is pain initiated or caused by a primary lesion or dysfunction in the nervous system. Peripheral neuropathic pain occurs when the lesion or dysfunction affects the peripheral nervous system and central neuropathic pain when the lesion or dysfunction affects the central nervous system (IASP).
Several types of therapeutic agents are currently used to treat neuropathic pain including, for example, tricyclic antidepressants (TCAs), serotonin and norepinephrine reuptake inhibitors (SNRIs), calcium channel ligands (e.g., gabapentin and pregabalin), topical lidocaine, and opioid agonists (e.g., morphine, oxycodone, methadone, levorphanol and tramadol). However, neuropathic pain can be very difficult to treat with no more than 40-60% of patients achieving, at best, partial relief of their pain (Dworkin et al. (2007) Pain 132:237-251). Moreover, all of the therapeutic agents currently used to treat neuropathic pain have various side effects (e.g., nausea, sedation, dizziness and somnolence) that can limit their effectiveness in some patients (Dworkin et al. supra).
SNRIs, such as duloxetine and venlafaxine, are often used as first line therapy for treating neuropathic pain. These agents inhibit the reuptake of both serotonin (5-hydroxytrypamine, 5-HT) and norepinephrine (NE) by binding to the serotonin and norepinephrine transporters (SERT and NET, respectively). However, both duloxetine and venlafaxine have higher affinity for SERT relative to NET (Vaishnavi et al. (2004) Biol. Psychiatry 55(3):320-322).
Preclinical studies suggest that inhibition of both SERT and NET may be necessary for maximally effective treatment of neuropathic and other chronic pain states (Jones et al. (2006) Neuropharmacology 51(7-8):1172-1180; Vickers et al. (2008) Bioorg. Med. Chem. Lett. 18:3230-3235; Fishbain et al. (2000) Pain Med. 1(4):310-316; and Mochizucki (2004) Human Psychopharmacology 19:S15-S19). However, in clinical studies, the inhibition of SERT has been reported to be related to nausea and other side effects (Greist et al. (2004) Clin. Ther. 26(9):1446-1455). Thus, therapeutic agents having more balanced SERT and NET affinity or slightly higher NET affinity are expected to be particularly useful for treating chronic pain while producing fewer side effects, such as nausea.
Thus, a need exists for novel compounds that are useful for treating chronic pain, such as neuropathic pain. In particular, a need exists for novel compounds that are useful for treating chronic pain and that have reduced side effects, such as nausea. A need also exists for novel dual-acting compounds that inhibit both SERT and NET with fairly high affinity (e.g., pK_i≧7.0 or K_i≦100 nM) and balanced inhibition (e.g., a SERT/NET binding K_iratio of 0.1 to 100).

SUMMARY OF THE INVENTION

The present invention provides novel compounds that have been found to possess serotonin reuptake inhibitory activity and norepinephrine reuptake inhibitory activity. Accordingly, compounds of the invention are expected to be useful and advantageous as therapeutic agents for those diseases and disorders that can be treated by inhibition of the serotonin and/or norepinephrine transporter, such as neuropathic pain.
One aspect of the invention relates to compounds of formula I:
where: X is —CH— or —N—; HAr is C_3-5heteroaryl; a is 0 or 1; R¹is halo or trifluoromethyl; and R^2-6are independently hydrogen, halo, —C_1-6alkyl, —CF₃, —C_2-6alkynyl, —O—C_1-6alkyl, —OCF₃, —C_1-4alkylene-O—C_1-4alkyl, —C_0-1alkylene-phenyl, —O—C_0-3alkylene-phenyl, —C_0-6alkylene-OH, —CN, —COOH, —CHO, —C(O)—C_1-6alkyl, —C(O)O—C_1-4alkyl, —CH₂SH, —S—C_1-6alkyl, —C_1-4alkylene-S—C_1-4alkyl, or —NO₂; or a pharmaceutically acceptable salt thereof.
Yet another aspect of the invention relates to pharmaceutical compositions comprising a pharmaceutically acceptable carrier and a compound of the invention. Such compositions may optionally contain other active agents such as anti-Alzheimer's agents, anticonvulsants, antidepressants, anti-Parkinson's agents, dual serotonin-norepinephrine reuptake inhibitors, non-steroidal anti-inflammatory agents, norepinephrine reuptake inhibitors, opioid agonists, opioid antagonists, selective serotonin reuptake inhibitors, sodium channel blockers, sympatholytics, and combinations thereof. Accordingly, in yet another aspect of the invention, a pharmaceutical composition comprises a compound of the invention, a second active agent, and a pharmaceutically acceptable carrier. Another aspect of the invention relates to a combination of active agents, comprising a compound of the invention and a second active agent. The compounds of the invention can be formulated together or separately from the additional agent(s). When formulated separately, a pharmaceutically acceptable carrier may be included with the additional agent(s). Thus, yet another aspect of the invention relates to a combination of pharmaceutical compositions, the combination comprising: a first pharmaceutical composition comprising a compound of formula I or a pharmaceutically acceptable salt thereof and a first pharmaceutically acceptable carrier; and a second pharmaceutical composition comprising a second active agent and a second pharmaceutically acceptable carrier. The invention also relates to a kit containing such pharmaceutical compositions, for example where the first and second pharmaceutical compositions are separate pharmaceutical compositions.
Compounds of the invention possess serotonin reuptake inhibitory activity and norepinephrine reuptake inhibitory activity, and are therefore expected to be useful as therapeutic agents for treating patients suffering from a disease or disorder that is treated by the inhibition of the serotonin and/or the norepinephrine transporter. Thus, one aspect of the invention relates to a method of treating: a pain disorder such as neuropathic pain or fibromyalgia; a depressive disorder such as major depression; an affective disorder such as an anxiety disorder; attention deficit hyperactivity disorder; a cognitive disorder such as dementia; stress urinary incontinence; chronic fatigue syndrome; obesity; or vasomotor symptoms associated with menopause, comprising administering to a patient a therapeutically effective amount of a compound of the invention.
Still another aspect of the invention relates to a method for inhibiting serotonin reuptake in a mammal comprising administering to the mammal, a serotonin transporter-inhibiting amount of a compound of the invention. Yet another aspect of the invention relates to a method for inhibiting norepinephrine reuptake in a mammal comprising administering to the mammal, a norepinephrine transporter-inhibiting amount of a compound of the invention. And another aspect of the invention is directed to a method for inhibiting serotonin reuptake and norepinephrine reuptake in a mammal comprising administering to the mammal, a serotonin transporter- and norepinephrine transporter-inhibiting amount of a compound of the invention.
Among the compounds of formula I, compounds of particular interest are those having an inhibitory constant (pK_i) at SERT and at NET greater than or equal to about 5.0; and in another embodiment having a pK_iat SERT and at NET greater than or equal to about 7.0. In another embodiment, compounds of interest have balanced SERT and NET activity, i.e., have the same pK_ivalue at both SERT and NET±0.5. Further compounds of particular interest are those having serotonin reuptake inhibition pIC₅₀values of greater than or equal to about 7.0 and norepinephrine reuptake inhibition pIC₅₀values of greater than or equal to about 7.0.
Since compounds of the invention possess serotonin reuptake inhibitory activity and norepinephrine reuptake inhibitory activity, such compounds are also useful as research tools. Accordingly, one aspect of the invention relates to methods of using the compounds of the invention as research tools, comprising conducting a biological assay using a compound of the invention. Compounds of the invention can also be used to evaluate new chemical compounds. Thus another aspect of the invention relates to a method of evaluating a test compound in a biological assay, comprising: (a) conducting a biological assay with a test compound to provide a first assay value; (b) conducting the biological assay with a compound of the invention to provide a second assay value; wherein step (a) is conducted either before, after or concurrently with step (b); and (c) comparing the first assay value from step (a) with the second assay value from step (b). Exemplary biological assays include a serotonin reuptake assay and a norepinephrine reuptake assay. Still another aspect of the invention relates to a method of studying a biological system or sample comprising serotonin transporters, norepinephrine transporters, or both, the method comprising: (a) contacting the biological system or sample with a compound of the invention; and (b) determining the effects caused by the compound on the biological system or sample.
The invention also relates to processes and intermediates useful for preparing compounds of the invention. Accordingly, one aspect of the invention relates to a process for preparing compounds of formula I, the process comprising deprotecting a compound of formula IV:
or a salt thereof, where P is an amino-protecting group to provide compounds of formula I, where X, HAr, a, and R¹through R⁶are as defined for formula I. In other aspects, the invention relates to novel intermediates used in such processes.
Yet another aspect of the invention relates to compounds of the invention for use in therapy, the use of compounds of the invention for the manufacture of medicaments, especially for the manufacture of medicaments useful for treating pain disorders, depressive disorders, affective disorders, attention deficit hyperactivity disorder, cognitive disorders, stress urinary incontinence, for inhibiting serotonin reuptake in a mammal, or for inhibiting norepinephrine reuptake in a mammal Still another aspect of the invention relates to the use of compounds of the invention as research tools. Other aspects and embodiments of the invention are disclosed herein.

DETAILED DESCRIPTION OF THE INVENTION

Definitions

When describing the compounds, compositions, methods and processes of the invention, the following terms have the following meanings unless otherwise indicated. Additionally, as used herein, the singular forms “a,” “an” and “the” include the corresponding plural forms unless the context of use clearly dictates otherwise. The terms “comprising”, “including,” and “having” are intended to be inclusive and mean that there may be additional elements other than the listed elements. All numbers expressing quantities of ingredients, properties such as molecular weight, reaction conditions, and so forth used herein are to be understood as being modified in all instances by the term “about,” unless otherwise indicated. Accordingly, the numbers set forth herein are approximations that may vary depending upon the desired properties sought to be obtained by the present invention. At least, and not as an attempt to limit the application of the doctrine of equivalents to the scope of the claims, each number should at least be construed in light of the reported significant digits and by applying ordinary rounding techniques.
The term “alkyl” means a monovalent saturated hydrocarbon group which may be linear or branched. Unless otherwise defined, such alkyl groups typically contain from 1 to 10 carbon atoms and include, for example, —C_1-2alkyl, —C_1-3alkyl, —C_1-4alkyl, —C_1-6alkyl, and —C_1-8alkyl. Representative alkyl groups include, by way of example, methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl, isobutyl, tert-butyl, n-pentyl, n-hexyl, n-heptyl, n-octyl, n-nonyl, n-decyl, and the like.
When a specific number of carbon atoms is intended for a particular term used herein, the number of carbon atoms is shown preceding the term as subscript. For example, the term “—C_1-6alkyl” means an alkyl group having from 1 to 6 carbon atoms, and the term “—C_1-4alkylene” means an alkylene group having from 1 to 4 carbon atoms, where the carbon atoms are in any acceptable configuration.
The term “alkylene” means a divalent saturated hydrocarbon group that may be linear or branched. Unless otherwise defined, such alkylene groups typically contain from 0 to 10 carbon atoms and include, for example, —C_0-1alkylene, —C_0-2alkylene, —C_0-3alkylene, —C_0-6alkylene, —C_1-4alkylene, —C_2-4alkylene and —C_1-6alkylene. Representative alkylene groups include, by way of example, methylene (—CH₂—), ethylene (—CH₂—CH₂—), propylene (—CH₂—CH₂—CH₂—, —CH₂(CH₃)—CH₂—, or —CH₂—CH₂(CH₃)—), butylene (—CH₂—CH₂—CH₂—CH₂—, —CH₂(CH₃)—CH₂—CH₂—, —CH₂—CH₂(CH₃)—CH₂—, —CH₂—CH₂—CH₂(CH₃)—, —CH₂—CH₂(CH₂CH₃)—, or —CH₂(CH₂CH₃)—CH₂—,), pentylene, and the like. It is understood that when the alkylene term includes zero carbons such as —C_0-1alkylene-, —C_0-3alkylene- or —C_0-6alkylene-, such terms are intended to include the absence of carbon atoms, i.e., the alkylene group is not present except for a covalent bond attaching the groups separated by the alkylene term.
The term “alkynyl” means a monovalent unsaturated hydrocarbon group which may be linear or branched and which has at least one, and typically 1, 2 or 3, carbon-carbon triple bonds. Unless otherwise defined, such alkynyl groups typically contain from 2 to 10 carbon atoms and include, for example, —C_2-4alkynyl, —C_2-6alkynyl and —C_3-10alkynyl. Representative alkynyl groups include, by way of example, ethynyl, n-propynyl, i-butynyl, n-but-2-ynyl, n-hex-3-ynyl, and the like.
The term “halo” means fluoro, chloro, bromo and iodo.
The term “C_3-5heteroaryl” means a monovalent aromatic group having a single ring and containing in the ring one or two heteroatoms selected from nitrogen, oxygen, and sulfur. Unless otherwise defined, such heteroaryl groups typically contain from 5 to 7 total ring atoms. Typically, the point of attachment is at any available carbon atom. Representative C₃heteroaryl groups include, oxazole (e.g., 2-oxazole and 5-oxazole), isoxazole (e.g., 3-isoxazole), thiazole (e.g., 2-thiazole and 4-thiazole), isothiazole (e.g., 3-isothiazole), imidazole (e.g., 2-imidazole), and pyrazole (e.g., 1H-pyrazol-3-yl). Representative C₄heteroaryl groups include, thiophene (e.g., 2-thienyl), furan (e.g., 2-furyl and 3-furyl), pyrrole (e.g., 3-pyrrolyl and 2H-pyrrol-3-yl), pyrazine, pyrimidine (e.g., 2,6-pyrimidinyl and 3,5-pyrimidinyl), and pyridazine (e.g., 3-pyridazinyl). Representative C₅heteroaryl groups include pyridine (e.g., 2-pyridyl, 3-pyridyl, and 4-pyridyl) and pyran (e.g., 2H-pyran and 4H-pyran).
As used herein, the phrase “of the formula”, “having the formula” or “having the structure” is not intended to be limiting and is used in the same way that the term “comprising” is commonly used.
The term “pharmaceutically acceptable” refers to a material that is not biologically or otherwise unacceptable when used in the invention. For example, the term “pharmaceutically acceptable carrier” refers to a material that can be incorporated into a composition and administered to a patient without causing unacceptable biological effects or interacting in an unacceptable manner with other components of the composition. Such pharmaceutically acceptable materials typically have met the required standards of toxicological and manufacturing testing, and include those materials identified as suitable inactive ingredients by the U.S. Food and Drug Administration.
The term “pharmaceutically acceptable salt” means a salt prepared from a base or an acid which is acceptable for administration to a patient, such as a mammal (e.g., salts having acceptable mammalian safety for a given dosage regime). However, it is understood that the salts covered by the invention are not required to be pharmaceutically acceptable salts, such as salts of intermediate compounds that are not intended for administration to a patient. Pharmaceutically acceptable salts can be derived from pharmaceutically acceptable inorganic or organic bases and from pharmaceutically acceptable inorganic or organic acids. In addition, when a compound of formula I contains both a basic moiety, such as an amine, and an acidic moiety such as a carboxylic acid, zwitterions may be formed and are included within the term “salt” as used herein. Salts derived from pharmaceutically acceptable inorganic bases include ammonium, calcium, copper, ferric, ferrous, lithium, magnesium, manganic, manganous, potassium, sodium, and zinc salts, and the like. Salts derived from pharmaceutically acceptable organic bases include salts of primary, secondary and tertiary amines, including substituted amines, cyclic amines, naturally-occurring amines, and the like, such as arginine, betaine, caffeine, choline, N,N′-dibenzylethylenediamine, diethylamine, 2-diethylaminoethanol, 2-dimethylaminoethanol, ethanolamine, ethylenediamine, N-ethylmorpholine, N-ethylpiperidine, glucamine, glucosamine, histidine, hydrabamine, isopropylamine, lysine, methylglucamine, morpholine, piperazine, piperadine, polyamine resins, procaine, purines, theobromine, triethylamine, trimethylamine, tripropylamine, tromethamine, and the like. Salts derived from pharmaceutically acceptable inorganic acids include salts of boric, carbonic, hydrohalic (hydrobromic, hydrochloric, hydrofluoric or hydroiodic), nitric, phosphoric, sulfamic and sulfuric acids. Salts derived from pharmaceutically acceptable organic acids include salts of aliphatic hydroxyl acids (e.g., citric, gluconic, glycolic, lactic, lactobionic, malic, and tartaric acids), aliphatic monocarboxylic acids (e.g., acetic, butyric, formic, propionic and trifluoroacetic acids), amino acids (e.g., aspartic and glutamic acids), aromatic carboxylic acids (e.g., benzoic, p-chlorobenzoic, diphenylacetic, gentisic, hippuric, and triphenylacetic acids), aromatic hydroxyl acids (e.g., o-hydroxybenzoic, p-hydroxybenzoic, 1-hydroxynaphthalene-2-carboxylic and 3-hydroxynaphthalene-2-carboxylic acids), ascorbic, dicarboxylic acids (e.g., fumaric, maleic, oxalic and succinic acids), glucuronic, mandelic, mucic, nicotinic, orotic, pamoic, pantothenic, sulfonic acids (e.g., benzenesulfonic, camphorsulfonic, edisylic, ethanesulfonic, isethionic, methanesulfonic, naphthalenesulfonic, naphthalene-1,5-disulfonic, naphthalene-2,6-disulfonic and p-toluenesulfonic acids), xinafoic acid, and the like.
The term “solvate” means a complex or aggregate formed by one or more molecules of a solute, e.g., a compound of formula I or a pharmaceutically acceptable salt thereof, and one or more molecules of a solvent. Such solvates are typically crystalline solids having a substantially fixed molar ratio of solute and solvent. Representative solvents include, by way of example, water, methanol, ethanol, isopropanol, acetic acid, and the like. When the solvent is water, the solvate formed is a hydrate.
The term “therapeutically effective amount” means an amount sufficient to effect treatment when administered to a patient in need thereof, i.e., the amount of drug needed to obtain the desired therapeutic effect. For example, a therapeutically effective amount for treating neuropathic pain is an amount of compound needed to, for example, reduce, suppress, eliminate or prevent the symptoms of neuropathic pain or to treat the underlying cause of neuropathic pain. On the other hand, the term “effective amount” means an amount sufficient to obtain a desired result, which may not necessary be a therapeutic result. For example, when studying a system comprising a norepinephrine transporter, an “effective amount” may be the amount needed to inhibit norepinephrine reuptake.
The term “treating” or “treatment” as used herein means the treating or treatment of a disease or medical condition (such as neuropathic pain) in a patient, such as a mammal (particularly a human), that includes one or more of the following: (a) preventing the disease or medical condition from occurring, i.e., prophylactic treatment of a patient; (b) ameliorating the disease or medical condition, i.e., eliminating or causing regression of the disease or medical condition in a patient; (c) suppressing the disease or medical condition, i.e., slowing or arresting the development of the disease or medical condition in a patient; or (d) alleviating the symptoms of the disease or medical condition in a patient. For example, the term “treating neuropathic pain” would include preventing neuropathic pain from occurring, ameliorating neuropathic pain, suppressing neuropathic pain, and alleviating the symptoms of neuropathic pain. The term “patient” is intended to include those mammals, such as humans, that are in need of treatment or disease prevention, that are presently being treated for disease prevention or treatment of a specific disease or medical condition, as well as test subjects in which compounds of the invention are being evaluated or being used in a assay, for example an animal model.
All other terms used herein are intended to have their ordinary meaning as understood by those of ordinary skill in the art to which they pertain.
In one aspect, this invention relates to compounds of formula I:
or a pharmaceutically acceptable salt thereof.
As used herein, the term “compound of the invention” or “compounds of the invention” include all compounds encompassed by formulas I, II, III, and IV, such as the species embodied in formulas Ia, Ib, Ic, Id, Ie, If, Ig, Ih, and Ii, and all other subspecies of such formulas. In addition, when a compound of the invention contain a basic or acidic group (e.g., amino or carboxyl groups), the compound can exist as a free base, free acid, a zwitterion, or in various salt forms. All such salt forms are included within the scope of the invention. Accordingly, those skilled in the art will recognize that reference to compounds herein, for example, reference to a “compound of the invention” or a “compound of formula I” includes a compound of formula I as well as pharmaceutically acceptable salts of that compound unless otherwise indicated. Furthermore, solvates are also included within the scope of this invention.
Compounds of the invention, as well as those compounds used in their synthesis, may also include isotopically-labeled compounds, i.e., where one or more atoms have been enriched with atoms having an atomic mass different from the atomic mass predominately found in nature. Examples of isotopes that may be incorporated into the compounds of the invention, for example, include, but are not limited to, ²H, ³H, ¹³C, ¹⁴C, ¹⁵N, ¹⁸O, ¹⁷O, ³⁵S, ³⁶Cl, and ¹⁸F. Of particular interest are compounds of formula I enriched in tritium or carbon-14 which can be used, for example, in tissue distribution studies; compounds of formula I enriched in deuterium especially at a site of metabolism resulting, for example, in compounds having greater metabolic stability; and compounds of formula I enriched in a positron emitting isotope, such as ¹¹C, ¹⁸F, ¹⁵O and ¹³N, which can be used, for example, in Positron Emission Topography (PET) studies.
The compounds of the invention have been found to possess serotonin reuptake inhibitory activity and norepinephrine reuptake inhibitory activity. Among other properties, such compounds are expected to be useful as therapeutic agents for treating chronic pain, such as neuropathic pain. By combining dual activity into a single compound, double therapy can be achieved, i.e., serotonin reuptake inhibitory activity and norepinephrine reuptake inhibitory activity, using a single active component. Since pharmaceutical compositions containing one active component are typically easier to formulate than compositions containing two active components, such single-component compositions provide a significant advantage over compositions containing two active components.
Many combined serotonin and norepinephrine reuptake inhibitors (SNRIs) are more selective for SERT than for NET. For example, milnacipran, duloxetine, and venlafaxine exhibit 2.5-fold, 10-fold, and 100-fold selectivity (measured as pK_i) for SERT over NET, respectively. Some, however, are less selective, such as bicifadine, which has a pK_iat SERT of 7.0 and a pK_iat NET of 6.7. Since it may be desirable to avoid selective compounds, in one embodiment of the invention the compounds have a more balanced SERT and NET activity, i.e., have the same pK_ivalue at both SERT and NET±0.5.
The compounds described herein have typically been named using the AutoNom feature of the commercially-available MDL® ISIS/Draw software (Symyx, Santa Clara, Calif.).

Representative Embodiments

The following substituents and values are intended to provide representative examples of various aspects and embodiments of the invention. These representative values are intended to further define and illustrate such aspects and embodiments and are not intended to exclude other embodiments or to limit the scope of the invention. In this regard, the representation that a particular value or substituent is preferred is not intended in any way to exclude other values or substituents from the invention unless specifically indicated.
In one aspect, this invention relates to compounds of formula I:
The integer a can be zero or 1. Thus, in one embodiment there are no R¹groups present. In another embodiment, there is one R¹group selected from halo and trifluoromethyl.
The R², R³, R⁴, R⁵, and R⁶groups are independently hydrogen, halo, —C_1-6alkyl, —CF₃, —C_2-6alkyl, —O—C_1-6alkyl, —OCF₃, —C_1-4alkylene-O—C_1-4alkyl, —C_0-1alkylene-phenyl, —O—C_0-3alkylene-phenyl, —C_0-6alkylene-OH, —CN, —COOH, —CHO, —C(O)—C_1-6alkyl, —C(O)O—C_1-4alkyl, —CH₂SH, —S—C_1-6alkyl, —C_1-4alkylene-S—C_1-4alkyl, or —NO₂. Each R group is independently hydrogen or —C_1-4alkyl.
In some embodiments of the invention, one or more positions on the phenyl ring are substituted with a non-hydrogen moiety. For example, one such embodiment may be described by stating that that “R²is a non-hydrogen moiety”. It is understood that this means that R²can be any of the non-hydrogen moieties defined in formula I, i.e., halo, —C_1-6alkyl, —CF₃, —C_2-6alkynyl, —O—C_1-6alkyl, —OCF₃, —C_1-4alkylene-O—C_1-4alkyl, —C_0-1alkylene-phenyl, —O—C_0-3alkylene-phenyl, —C_0-6alkylene-OH, —CN, —COOH, —CHO, —C(O)—C_1-6alkyl, —C(O)O—C_1-4alkyl, —CH₂SH, —S—C_1-6alkyl, —C_1-4alkylene-S—C_1-4alkyl, and —NO₂.
Exemplary halo groups include fluoro, chloro, bromo, and iodo. Exemplary —C_1-6alkyl groups include —CH₃, —CH₂CH₃, and —CH(CH₃)₂. Exemplary —C_2-6alkyl groups include —CH═CH₂. Exemplary —O—C_1-6alkyl groups include —OCH₃, —O—CH₂CH₃, and —OCH(CH₃)₂. Exemplary —C_1-4alkylene-O—C_1-4alkyl groups include —CH₂—OCH₃and —CH₂—OCH₂CH₃. Exemplary —C_0-1alkylene-phenyl groups include phenyl and benzyl. Exemplary —O—C_0-3alkylene-phenyl groups include —O-phenyl and —O-benzyl. Exemplary —C_0-6alkylene-OH groups include —OH and —CH₂OH. Exemplary —C(O)—C_1-6alkyl groups include —C(O)CH₃and —C(O)CH₂CH₃. Exemplary —C(O)O—C_1-4alkyl groups include —C(O)OCH₃, —C(O)OCH₂CH₃, and —C(O)OCH(CH₃)₂. Exemplary —S—C_1-6alkyl groups include —SCH₃. Exemplary —C_1-4alkylene-S—C_1-4alkyl groups include —CH₂—S—CH₃.
In one particular embodiment, R²is hydrogen or halo (e.g., fluoro or chloro). In one embodiment, R³is hydrogen or halo (e.g., fluoro or chloro). In yet another embodiment, R⁴is hydrogen or halo (e.g., fluoro or chloro). In another embodiment, R⁵is hydrogen or halo (e.g., fluoro). In yet another embodiment, R⁶is hydrogen or halo (e.g., fluoro or chloro).
The “X” group is —CH— or —N—. In one particular embodiment, the “X” group is —CH—.
The “HAr” ring is a C_3-5heteroaryl group. In one embodiment of the invention, the “HAr” ring is a pyridine, such as 2-pyridyl, 3-pyridyl, 4-pyridyl, or 5-pyridyl, which can be depicted as formulas Ia-Id, respectively:
In another embodiment of the invention, the “HAr” ring is a furan, such as 2-furyl, 3-furyl, or 4-furyl, which can be depicted as formulas Ie-Ig, respectively:
In yet another embodiment of the invention, the “HAr” ring is a thiophene, such as 2-thienyl, 3-thienyl, or 4-thienyl, which can be depicted as formulas Ih-Ii, respectively:
In one embodiment, R^2-6are independently hydrogen or halo. Exemplary combinations include:

- R²-R⁶are hydrogen;
- R²is fluoro and R³-R⁶are hydrogen;
- R²is hydrogen, R³is chloro, and R⁴-R⁶are hydrogen;
- R²is fluoro, R³-R⁵are hydrogen, and R⁶is fluoro;
- R²is fluoro, R³-R⁵are hydrogen, and R⁶is chloro;
- R²is chloro, R³-R⁵are hydrogen, and R⁶is chloro;
- R²is chloro, R³-R⁵are hydrogen, and R⁶is fluoro;
- R²and R³are fluoro, R⁴and R⁵are hydrogen, and R⁶is fluoro;
- R²is fluoro, R³is hydrogen, R⁴is fluoro, R⁵is hydrogen, and R⁶is fluoro;
- R²is fluoro, R³is hydrogen, R⁴is chloro, R⁵is hydrogen, and R⁶is fluoro; and
- R²is fluoro, R³and R⁴are hydrogen, R⁵is fluoro, and R⁶is chloro.
  In one aspect, these embodiments have formulas (Ia)-(Ii).

In one particular embodiment, the compounds of the invention have formula II:
where: X, HAr, a, R¹, and R³through R⁶are as defined for formula I; or a pharmaceutically acceptable salt thereof. In one specific embodiment of formula II: X is —CH—; a is 0; HAr is selected from 2-pyridyl, 3-pyridyl, 4-pyridyl, 5-pyridyl, 2-furyl, 4-furyl, 2-thienyl, 3-thienyl, and 4-thienyl; and R^3-6are independently hydrogen or halo.
In one particular embodiment, the compounds of the invention have formula III:
where: HAr and R²through R⁶are as defined for formula I; or a pharmaceutically acceptable salt thereof. In one specific embodiment of formula III, HAr is selected from 2-pyridyl, 3-pyridyl, 4-pyridyl, 5-pyridyl, 2-furyl, 4-furyl, 2-thienyl, 3-thienyl, and 4-thienyl; and R^2-6are independently hydrogen or halo; or a pharmaceutically acceptable salt thereof.

General Synthetic Procedures

Compounds of the invention can be prepared from readily available starting materials using the following general methods, the procedures set forth in the Examples, or by using other methods, reagents, and starting materials that are known to those skilled in the art. Although the following procedures may illustrate a particular embodiment of the invention, it is understood that other embodiments of the invention can be similarly prepared using the same or similar methods or by using other methods, reagents and starting materials known to those of ordinary skill in the art. It will also be appreciated that where typical or preferred process conditions (i.e., reaction temperatures, times, mole ratios of reactants, solvents, pressures, etc.) are given, other process conditions can also be used unless otherwise stated. While optimum reaction conditions will typically vary depending on various reaction parameters such as the particular reactants, solvents and quantities used, those of ordinary skill in the art can readily determine suitable reaction conditions using routine optimization procedures.
Additionally, as will be apparent to those skilled in the art, conventional protecting groups may be necessary or desired to prevent certain functional groups from undergoing undesired reactions. The choice of a suitable protecting group for a particular functional group as well as suitable conditions and reagents for protection and deprotection of such functional groups are well-known in the art. Protecting groups other than those illustrated in the procedures described herein may be used, if desired. For example, numerous protecting groups, and their introduction and removal, are described in T. W. Greene and G. M. Wuts, Protecting Groups in Organic Synthesis, Third Edition, Wiley, New York, 1999, and references cited therein.
More particularly, in the schemes below, P represents an “amino-protecting group,” a term used herein to mean a protecting group suitable for preventing undesired reactions at an amino group. Representative amino-protecting groups include, but are not limited to, t-butoxycarbonyl (Boc), trityl (Tr), benzyloxycarbonyl (Cbz), 9-fluorenylmethoxycarbonyl (Fmoc), formyl, benzyl, and the like. Standard deprotection techniques and reagents such as TFA in DCM or HCl in 1,4-dioxane, methanol, or ethanol, are used to remove protecting groups, when present. For example, a Boc group can be removed using an acidic reagent such as hydrochloric acid, trifluoroacetic acid, and the like; while a Cbz group can be removed by employing catalytic hydrogenation conditions such as H₂(1 atm), 10% Pd/C in an alcoholic solvent. The schemes are illustrated with Boc as the protecting group.
Suitable inert diluents or solvents for use in these schemes include, by way of illustration and not limitation, tetrahydrofuran (THF), acetonitrile, N,N-dimethylformamide (DMF), dimethylsulfoxide (DMSO), toluene, dichloromethane (DCM), chloroform (CHCl₃), and the like.
All reactions are typically conducted at a temperature within the range of about −78° C. to about 110° C., for example at room temperature. Reactions may be monitored by use of thin layer chromatography (TLC), high performance liquid chromatography (HPLC), and/or LCMS until completion. Reactions may be complete in minutes, may take hours, typically from 1-2 hours and up to 48 hours, or days, such as up to 3-4 days. Upon completion, the resulting mixture or reaction product may be further treated in order to obtain the desired product. For example, the resulting mixture or reaction product may be subjected to one or more of the following procedures: dilution (for example with saturated NaHCO₃); extraction (for example, with ethyl acetate, CHCl₃, DCM, aqueous HCl); washing (for example, with DCM, saturated aqueous NaCl, or saturated aqueous NaHCO₃); drying (for example, over MgSO₄or Na₂SO₄, or in vacuo); filtration; being concentrated (for example, in vacuo); being redissolved (for example in a 1:1 acetic acid:H₂O solution); and/or purification (for example by preparative HPLC, reverse phase preparative HPLC, or crystallization).
By way of illustration, compounds of the invention can be prepared by Scheme A, B, or C, which are detailed in the examples.
Compounds of the invention may be prepared using the Mitsunobu coupling reaction (Mitsunobu and Yamada (1967) M. Bull. Chem. Soc. JPN. 40:2380-2382), followed by deprotection of the amine, as shown in Scheme A. This reaction is typically conducted using standard Mitsunobu coupling conditions, using a redox system containing an azodicarboxylate such as diisopropyl azodicarboxylate (DIAD) or diethyl azodicarboxylate (DEAD), and a phosphine catalyst such as triphenylphosphine (PPh₃).
The method of Scheme B involves first converting the hydroxyl group into a leaving group. Representative leaving groups include chloro, bromo and iodo groups; sulfonic ester groups, such as mesylate, tosylate, brosylate, nosylate, and the like; and acyloxy groups, such as acetoxy, trifluoroacetoxy, and the like. For example, the alcohol can undergo tosylation with an appropriate reagent such as p-toluenesulfonyl chloride (TsCl) in a suitable base such as DABCO (1,4-diazabicyclo[2.2.2]octane or triethylenediamine), to form the tosylate ester. See, for example, Hartung et al. (1997) Synthesis 12:1433-1438. This tosylate ester is then coupled with the desired phenol by nucleophilic displacement. The protected amine is then deprotected to yield a compound of the invention.
The method of Scheme C involves first coupling the starting material with the desired substituted phenyl (where X is —O—SO₂—CF₃(i.e., triflate) or iodide) under modified Ullmann reaction conditions. The modified Ullmann reaction is typically conducted in the presence of a copper(I) iodide/1,10-phenanthroline catalyst and a base such as cesium carbonate, in an appropriate solvent such as toluene or DMF. The resulting product is then deprotected to yield a compound of the invention. This final step is conducted under standard deprotection conditions, which will vary depending upon the protecting group used. For example, removal of the BOC group can be done using trifluoroacetic acid and dichloromethane. The triflate compound is either commercially available, or is readily synthesized by techniques that are well known in the art.
The starting material:
where X is carbon can be prepared by techniques that are well known in the art and the synthesis of each exemplary HAr group is depicted in the Preparations. The starting material where X is nitrogen can be prepared, for example, by techniques described in Louërat et al. (2005)Tetrahedron 61(20):4761-4768, Romero et al. (1994) J. Med. Chem. 37(7):999-1014, and Tran et al. (2007) J. Med. Chem. 50(25):6356-6366. The phenol compound:
is either commercially available, or is readily synthesized by techniques that are well known in the art.
If desired, pharmaceutically acceptable salts of the compounds of formula I or II can be prepared by contacting the free acid or base form of a compound of formula I or II, respectively, with a pharmaceutically acceptable base or acid.
Certain of the intermediates described herein are believed to be novel and accordingly, such compounds are provided as further aspects of the invention including, for example, compounds of formula IV:
or a salt thereof, where P represents an amino-protecting group, particularly t-butoxycarbonyl (Boc) where X, HAr, a, and R^1-6are as defined for formulas I, II, or III. In one embodiment of the invention, compounds of the invention can be prepared by deprotecting compounds of formula IV to provide compounds of formula I, II, or III, or a pharmaceutically acceptable salt thereof.
Further details regarding specific reaction conditions and other procedures for preparing representative compounds of the invention or intermediates thereof are described in the Examples set forth below.

Utility

Compounds of the invention possess serotonin and norepinephrine reuptake inhibitory activity. Thus, these compounds are expected to have therapeutic utility as combined serotonin and norepinephrine reuptake inhibitors (SNRIs). In one embodiment, compounds of the invention possess equal or approximately equal serotonin reuptake inhibitory activity and norepinephrine reuptake inhibitory activity.
The inhibition constant (K_i) of a compound is the concentration of ligand in a radioligand binding inhibition assay that would occupy 50% of the transporters if no radioligand were present. K_ivalues can be determined from radioligand binding studies with ³H-nisoxetine (for the norepinephrine transporter, NET) and ³H-citalopram (for the serotonin transporter, SERT), as described in Assay 1. These K_ivalues are derived from IC₅₀values in the binding assay using the Cheng-Prusoff equation and the K_dof the radioligand (Cheng & Prusoff (1973) Biochem. Pharmacol. 22(23):3099-3108). Functional IC₅₀values can be determined in the functional inhibition of uptake assays described in Assay 2. These IC₅₀values can be converted to K_ivalues using the Cheng-Prusoff equation and the K_mof the transmitter for the transporter. It is noted however, that the uptake assay conditions described in Assay 2 are such that the IC₅₀values are very close to the K_ivalues, should a mathematical conversion be desired, since the neurotransmitter concentration (5-HT or NE) used in the assay is well below its K_mfor the respective transporter.
One measure of the affinity of a compound for SERT or NET is the inhibitory constant (pK_i) for binding to the transporter. The pK_ivalue is the negative logarithm to base 10 of the K_i. Compounds of the invention of particular interest are those having a pK_iat SERT greater than or equal to about 5.0; and in another embodiment greater than or equal to about 7.0. Compounds of the invention of particular interest also include those having a pK_iat NET greater than or equal to about 5.0; and in another embodiment greater than or equal to about 7.0. In another embodiment, compounds of interest have a pK_iat both SERT and NET greater than or equal to about 7.0. In another embodiment, compounds of interest have balanced SERT and NET activity, i.e., have the same pK_ivalue at both SERT and NET±0.5. Such values can be determined by techniques that are well known in the art, as well as in the assays described herein.
In one embodiment, compounds of the invention exhibit a NET pK_i≧7 and: a SERT K_i/NET K_iin the range of 0.1 to 100; a SERT K_i/NET K_iin the range of 0.3 to 100; a SERT K_i/NET K_iin the range of 0.3 to 10; or a SERT K_i/NET K_iin the range of 0.1 to 30.
Another measure of serotonin and norepinephrine reuptake inhibition is the pIC₅₀value. In one embodiment, compounds of interest have a serotonin reuptake inhibition pIC₅₀value of greater than or equal to about 7.0 and a norepinephrine reuptake inhibition pIC₅₀value of greater than or equal to about 7.0. In one particular embodiment, the compounds have a serotonin reuptake inhibition pIC₅₀value of greater than or equal to about 7.0 and a norepinephrine reuptake inhibition pIC₅₀value of greater than or equal to about 7.0. In one particular embodiment, the compounds of the invention have balanced pIC₅₀values, i.e., have the same pIC₅₀value at both SERT and NET±0.5.
In another embodiment, compounds of the invention are selective for inhibition of SERT and NET over the dopamine transporter (DAT). For example in this embodiment, compounds of particular interest are those that exhibit a binding affinity for SERT and NET that is at least 5 times higher than the binding affinity for DAT, or that is at least 10 times higher than for DAT, or at least 20 or 30 times higher than for DAT. In another embodiment, the compounds do not exhibit significant DAT inhibition. In still another embodiment, the compounds exhibit less than 50% inhibition of DAT activity when measured at a concentration of 794 nM. Under the assay conditions used, a compound which exhibits ≦50% inhibition would have an estimated pK_ivalue at DAT of ≦6.1.
In still another embodiment, compounds of the invention possess dopamine reuptake inhibitory activity as well as SERT and NET activity. For example in this embodiment, compounds of particular interest are those that exhibit a pK_iat SERT and NET greater than or equal to about 7.0, and a pK_iat DAT greater than or equal to about 7.0.
It is noted that in some cases, compounds of the invention may possess either weak serotonin reuptake inhibitory activity or weak norepinephrine reuptake inhibitory activity. In these cases, those of ordinary skill in the art will recognize that such compounds still have utility as primarily either a NET inhibitor or a SERT inhibitor, respectively, or will have utility as research tools.
Exemplary assays to determine the serotonin and/or norepinephrine reuptake inhibiting activity of compounds of the invention include by way of illustration and not limitation, assays that measure SERT and NET binding, for example, as described in Assay 1 and in Tsuruda et al. (2010) Journal of Pharmacological and Toxicological Methods 61(2):192-204. In addition, it is useful to understand the level of DAT binding and uptake in an assay such as that described in Assay 1. Useful secondary assays include neurotransmitter uptake assays to measure inhibition of serotonin and norepinephrine uptake into cells expressing the respective human or rat recombinant transporter (hSERT, hNET, or hDAT) as described in Assay 2, and ex vivo radioligand binding and neurotransmitter uptake assays that are used to determine the in vivo occupancy of SERT, NET and DAT in tissue as described in Assay 3. Other assays that are useful to evaluate pharmacological properties of test compounds include those listed in Assay 4. Exemplary in vivo assays include the formalin paw test described in Assay 5, which is a reliable predictor of clinical efficacy for the treatment of neuropathic pain, and the spinal nerve ligation model described in Assay 6. The aforementioned assays are useful in determining the therapeutic utility, for example, the neuropathic pain relieving activity, of compounds of the invention. Other properties and utilities of compounds of the invention can be demonstrated using various in vitro and in vivo assays well-known to those skilled in the art.
Compounds of the invention are expected to be useful for the treatment and/or prevention of medical conditions in which the regulation of monoamine transporter function is implicated, in particular those conditions mediated by or responsive to the inhibition of serotonin and norepinephrine reuptake. Thus it is expected that patients suffering from a disease or disorder that is treated by the inhibition of the serotonin and/or the norepinephrine transporter can be treated by administering a therapeutically effective amount of a serotonin and norepinephrine reuptake inhibitor of the invention. Such medical conditions include, by way of example: pain disorders such as neuropathic pain, fibromyalgia, and chronic pain; depressive disorders such as major depression; affective disorders such as an anxiety disorder; attention deficit hyperactivity disorder; cognitive disorders such as dementia; stress urinary incontinence; chronic low back pain; and osteoarthritis.
The amount of active agent administered per dose or the total amount administered per day may be predetermined or it may be determined on an individual patient basis by taking into consideration numerous factors, including the nature and severity of the patient's condition, the condition being treated, the age, weight, and general health of the patient, the tolerance of the patient to the active agent, the route of administration, pharmacological considerations such as the activity, efficacy, pharmacokinetics and toxicology profiles of the active agent and any secondary agents being administered, and the like. Treatment of a patient suffering from a disease or medical condition (such as neuropathic pain) can begin with a predetermined dosage or a dosage determined by the treating physician, and will continue for a period of time necessary to prevent, ameliorate, suppress, or alleviate the symptoms of the disease or medical condition. Patients undergoing such treatment will typically be monitored on a routine basis to determine the effectiveness of therapy. For example, in treating neuropathic pain, a measure of the effectiveness of treatment may involve assessment of the patient's quality of life, e.g., improvements in the patient's sleeping patterns, work attendance, ability to exercise and be ambulatory, etc. Pain scales, operating on a point basis, may also be used to help evaluate a patient's pain level. Indicators for the other diseases and conditions described herein, are well-known to those skilled in the art, and are readily available to the treating physician. Continuous monitoring by the physician will insure that the optimal amount of active agent will be administered at any given time, as well as facilitating the determination of the duration of treatment. This is of particular value when secondary agents are also being administered, as their selection, dosage, and duration of therapy may also require adjustment. In this way, the treatment regimen and dosing schedule can be adjusted over the course of therapy so that the lowest amount of active agent that exhibits the desired effectiveness is administered and, further, that administration is continued only so long as is necessary to successfully treat the disease or medical condition.

Pain Disorders

SNRIs have been shown to have a beneficial effect on pain such as painful diabetic neuropathy (duloxetine, Goldstein et al. (2005) Pain 116:109-118; venlafaxine, Rowbotham et al. (2004) Pain 110:697-706), fibromyalgia (duloxetine, Russell et al. (2008) Pain 136(3):432-444; milnacipran, Vitton et al. (2004) Human Psychopharmacology 19:S27-S35), and migraine (venlafaxine, Ozyalcin et al. (2005) Headache 45(2):144-152). Thus, one embodiment of the invention relates to a method for treating a pain disorder, comprising administering to a patient a therapeutically effective amount of a compound of the invention. Typically, the therapeutically effective amount will be the amount that is sufficient to relieve the pain. Exemplary pain disorders include, by way of illustration, acute pain, persistent pain, chronic pain, inflammatory pain, and neuropathic pain. More specifically, these include pain associated with or caused by: arthritis; back pain including chronic low back pain; cancer, including tumor related pain (e.g., bone pain, headache, facial pain or visceral pain) and pain associated with cancer therapy (e.g., post-chemotherapy syndrome, chronic post-surgical pain syndrome and post-radiation syndrome); carpal tunnel syndrome; fibromyalgia; headaches including chronic tension headaches; inflammation associated with polymyalgia, rheumatoid arthritis and osteoarthritis; migraine; neuropathic pain including complex regional pain syndrome; overall pain; post-operative pain; shoulder pain; central pain syndromes, including post-stroke pain, and pain associated with spinal cord injuries and multiple sclerosis; phantom limb pain; pain associated with Parkinson's disease; and visceral pain (e.g., irritable bowel syndrome). Of particular interest is the treatment of neuropathic pain, which includes diabetic peripheral neuropathy (DPN), HIV-related neuropathy, post-herpetic neuralgia (PHN), and chemotherapy-induced peripheral neuropathy. When used to treat pain disorders such as neuropathic pain, compounds of the invention may be administered in combination with other therapeutic agents, including anticonvulsants, antidepressants, muscle relaxants, NSAIDs, opioid agonists, opioid antagonists, selective serotonin reuptake inhibitors, sodium channel blockers, and sympatholytics. Exemplary compounds within these classes are described herein.

Depressive Disorders

Another embodiment of the invention relates to a method of treating a depressive disorder, comprising administering to a patient a therapeutically effective amount of a compound of the invention. Typically, the therapeutically effective amount will be the amount that is sufficient to alleviate depression and provide a sense of general well-being. Exemplary depressive disorders include, by way of illustration and not limitation: depression associated with Alzheimer's disease, bipolar disorder, cancer, child abuse, infertility, Parkinson's disease, postmyocardial infarction, and psychosis; dysthymia; grumpy or irritable old man syndrome; induced depression; major depression; pediatric depression; postmenopausal depression; post partum depression; recurrent depression; single episode depression; and subsyndromal symptomatic depression. Of particular interest is the treatment of major depression. When used to treat depressive disorders, compounds of the invention may be administered in combination with other therapeutic agents, including antidepressants and dual serotonin-norepinephrine reuptake inhibitors. Exemplary compounds within these classes are described herein.

Affective Disorders

Another embodiment of the invention relates to a method of treating an affective disorder, comprising administering to a patient a therapeutically effective amount of a compound of the invention. Exemplary affective disorders include, by way of illustration and not limitation: anxiety disorders such as general anxiety disorder; avoidant personality disorder; eating disorders such as anorexia nervosa, bulimia nervosa and obesity; obsessive compulsive disorder; panic disorder; personality disorders such as avoidant personality disorder and attention deficit hyperactivity disorder (ADHD); post-traumatic stress syndrome; phobias such as agoraphobia, as well as simple and other specific phobias, and social phobia; premenstrual syndrome; psychotic disorders, such as schizophrenia and mania; seasonal affective disorder; sexual dysfunction, including premature ejaculation, male impotence, and female sexual dysfunction such as female sexual arousal disorder; social anxiety disorder; and substance abuse disorders, including chemical dependencies such as addictions to alcohol, benzodiazepines, cocaine, heroin, nicotine and phenobarbital, as well as withdrawal syndromes that may arise from these dependencies. When used to treat affective disorders, compounds of the invention may be administered in combination with other therapeutic agents, including antidepressants. Exemplary compounds within these classes are described herein.
Atomoxetine, which is 10-fold NET selective, is approved for attention deficit hyperactivity disorder (ADHD) therapy, and clinical studies have shown that the SNRI, venlafaxine, can also have a beneficial effect in treating ADHD (Mukaddes et al. (2002) Eur. Neuropsychopharm. 12(Supp 3):421). Thus, the compounds of the invention are also expected to be useful in methods for treating attention deficit hyperactivity disorder by administering to a patient a therapeutically effective amount of a compound of the invention. When used to treat depression, compounds of the invention may be administered in combination with other therapeutic agents, including antidepressants. Exemplary compounds within these classes are described herein.

Cognitive Disorders

Another embodiment of the invention relates to a method of treating a cognitive disorder, comprising administering to a patient a therapeutically effective amount of a compound of the invention. Exemplary cognitive disorders include, by way of illustration and not limitation: dementia, which includes degenerative dementia (e.g., Alzheimer's disease, Creutzfeldt-Jakob disease, Huntingdon's chorea, Parkinson's disease, Pick's disease, and senile dementia), vascular dementia (e.g., multi-infarct dementia), and dementia associated with intracranial space occupying lesions, trauma, infections and related conditions (including HIV infection), metabolism, toxins, anoxia and vitamin deficiency; and mild cognitive impairment associated with ageing, such as age associated memory impairment, amnesiac disorder and age-related cognitive decline. When used to treat cognitive disorders, compounds of the invention may be administered in combination with other therapeutic agents, including anti-Alzheimer's agents and anti-Parkinson's agents. Exemplary compounds within these classes are described herein.

Other Disorders

SNRIs have also been shown to be effective for the treatment of stress urinary incontinence (Dmochowski (2003) Journal of Urology 170(4): 1259-1263). Thus, another embodiment of the invention relates to a method for treating stress urinary incontinence, comprising administering to a patient a therapeutically effective amount of a compound of the invention. When used to treat stress urinary incontinence, compounds of the invention may be administered in combination with other therapeutic agents, including anticonvulsants. Exemplary compounds within these classes are described herein.
Duloxetine, an SNRI, is undergoing clinical trials for evaluating its efficacy in treating chronic fatigue syndrome, and has recently been shown to be effective in treating fibromyalgia (Russell et al. (2008) Pain 136(3):432-444). The compounds of the invention, due to their ability to inhibit SERT and NET, are also expected to have this utility, and another embodiment of the invention relates to a method for treating chronic fatigue syndrome, comprising administering to a patient a therapeutically effective amount of a compound of the invention.
Sibutramine, a norepinephrine and dopamine reuptake inhibitor, has been shown to be useful in treating obesity (Wirth et al. (2001) JAMA 286(11):1331-1339). The compounds of the invention, due to their ability to inhibit NET, are also expected to have this utility, and another embodiment of the invention relates to a method for treating obesity, comprising administering to a patient a therapeutically effective amount of a compound of the invention.
Desvenlafaxine, an SNRI, has been shown to relieve vasomotor symptoms associated with menopause (Deecher et al. (2007) Endocrinology 148(3):1376-1383). The compounds of the invention, due to their ability to inhibit SERT and NET, are also expected to have this utility, and another embodiment of the invention relates to a method for treating vasomotor symptoms associated with menopause, comprising administering to a patient a therapeutically effective amount of a compound of the invention.

Research Tools

Since compounds of the invention possess both serotonin reuptake inhibition activity and norepinephrine reuptake inhibition activity, such compounds are also useful as research tools for investigating or studying biological systems or samples having serotonin or norepinephrine transporters. Any suitable biological system or sample having serotonin and/or norepinephrine transporters may be employed in such studies which may be conducted either in vitro or in vivo. Representative biological systems or samples suitable for such studies include, but are not limited to, cells, cellular extracts, plasma membranes, tissue samples, isolated organs, mammals (such as mice, rats, guinea pigs, rabbits, dogs, pigs, humans, and so forth), and the like, with mammals being of particular interest. In one particular embodiment of the invention, serotonin reuptake in a mammal is inhibited by administering a serotonin reuptake-inhibiting amount of a compound of the invention. In another particular embodiment, norepinephrine reuptake in a mammal is inhibited by administering a norepinephrine reuptake-inhibiting amount of a compound of the invention. Compounds of the invention can also be used as research tools by conducting biological assays using such compounds.
When used as a research tool, a biological system or sample comprising a serotonin transporter and/or a norepinephrine transporter is typically contacted with a serotonin reuptake-inhibiting or norepinephrine reuptake-inhibiting amount of a compound of the invention. After the biological system or sample is exposed to the compound, the effects of inhibiting serotonin reuptake and/or norepinephrine reuptake are determined using conventional procedures and equipment. Exposure encompasses contacting cells or tissue with the compound, administering the compound to a mammal, for example by i.p. or i.v. administration, and so forth. This determining step may comprise measuring a response, i.e., a quantitative analysis or may comprise an observation, i.e., a qualitative analysis. Measuring a response involves, for example, determining the effects of the compound on the biological system or sample using conventional procedures and equipment, such as serotonin and norepinephrine reuptake assays. The assay results can be used to determine the activity level as well as the amount of compound necessary to achieve the desired result, i.e., a serotonin reuptake-inhibiting and a norepinephrine reuptake-inhibiting amount.
Additionally, compounds of the invention can be used as research tools for evaluating other chemical compounds, and thus are also useful in screening assays to discover, for example, new compounds having both serotonin reuptake-inhibiting activity and norepinephrine reuptake-inhibiting activity. In this manner, compounds of the invention are used as standards in an assay to allow comparison of the results obtained with a test compound and with compounds of the invention to identify those test compounds that have about equal or superior reuptake-inhibiting activity, if any. For example, reuptake data for a test compound or a group of test compounds is compared to the reuptake data for a compound of the invention to identify those test compounds that have the desired properties, e.g., test compounds having reuptake-inhibiting activity about equal or superior to a compound of the invention, if any. This aspect of the invention includes, as separate embodiments, both the generation of comparison data (using the appropriate assays) and the analysis of the test data to identify test compounds of interest. Thus, a test compound can be evaluated in a biological assay, by a method comprising the steps of: (a) conducting a biological assay with a test compound to provide a first assay value; (b) conducting the biological assay with a compound of the invention to provide a second assay value; wherein step (a) is conducted either before, after or concurrently with step (b); and (c) comparing the first assay value from step (a) with the second assay value from step (b). Exemplary biological assays include serotonin and norepinephrine reuptake assays.

Pharmaceutical Compositions and Formulations

Compounds of the invention are typically administered to a patient in the form of a pharmaceutical composition or formulation. Such pharmaceutical compositions may be administered to the patient by any acceptable route of administration including, but not limited to, oral, rectal, vaginal, nasal, inhaled, topical (including transdermal) and parenteral modes of administration. Further, the compounds of the invention may be administered, for example orally, in multiple doses per day (e.g., twice, three times or four times daily), in a single daily dose, in a twice daily dose, in a single weekly dose, and so forth. It will be understood that any form of the compounds of the invention, (i.e., free base, pharmaceutically acceptable salt, solvate, etc.) that is suitable for the particular mode of administration can be used in the pharmaceutical compositions discussed herein.
Accordingly, in one embodiment, the invention relates to a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of the invention. The compositions may contain other therapeutic and/or formulating agents if desired. When discussing compositions, the “compound of the invention” may also be referred to herein as the “active agent,” to distinguish it from other components of the formulation, such as the carrier. Thus, it is understood that the term “active agent” includes compounds of formula I as well as pharmaceutically acceptable salts and solvates of that compound.
Pharmaceutical compositions of the invention typically contain a therapeutically effective amount of a compound of the invention. Those skilled in the art will recognize, however, that a pharmaceutical composition may contain more than a therapeutically effective amount, i.e., bulk compositions, or less than a therapeutically effective amount, i.e., individual unit doses designed for multiple administration to achieve a therapeutically effective amount. Typically, the composition will contain from about 0.01-95 wt % of active agent, including, from about 0.01-30 wt %, such as from about 0.01-10 wt %, with the actual amount depending upon the formulation itself, the route of administration, the frequency of dosing, and so forth. In one embodiment, a composition suitable for an oral dosage form, for example, may contain about 5-70 wt %, or from about 10-60 wt % of active agent. In one exemplary embodiment, a pharmaceutical composition contains from about 1 to 20 mg of active agent, including from about 1 to 15 mg of active agent and from about 1 to 10 mg of active agent. In another exemplary embodiment, a pharmaceutical composition contains from about 5 to 20 mg of active agent, including from about 7.5 to 15 mg of active agent. For example the active agent may be formulated in 1 mg and 10 mg unit doses.
Any conventional carrier or excipient may be used in the pharmaceutical compositions of the invention. The choice of a particular carrier or excipient, or combinations of carriers or excipients, will depend on the mode of administration being used to treat a particular patient or type of medical condition or disease state. In this regard, the preparation of a suitable composition for a particular mode of administration is well within the scope of those skilled in the pharmaceutical arts. Additionally, carriers or excipients used in such compositions are commercially available. By way of further illustration, conventional formulation techniques are described in Remington: The Science and Practice of Pharmacy, 20^thEdition, Lippincott Williams & White, Baltimore, Md. (2000); and H. C. Ansel et al., Pharmaceutical Dosage Forms and Drug Delivery Systems, 7^thEdition, Lippincott Williams & White, Baltimore, Md. (1999).
Representative examples of materials which can serve as pharmaceutically acceptable carriers include, but are not limited to, the following: sugars, such as lactose, glucose and sucrose; starches, such as corn starch and potato starch; cellulose, such as microcrystalline cellulose, and its derivatives, such as sodium carboxymethyl cellulose, ethyl cellulose and cellulose acetate; powdered tragacanth; malt; gelatin; talc; excipients, such as cocoa butter and suppository waxes; oils, such as peanut oil, cottonseed oil, safflower oil, sesame oil, olive oil, corn oil and soybean oil; glycols, such as propylene glycol; polyols, such as glycerin, sorbitol, mannitol and polyethylene glycol; esters, such as ethyl oleate and ethyl laurate; agar; buffering agents, such as magnesium hydroxide and aluminum hydroxide; alginic acid; pyrogen-free water; isotonic saline; Ringer's solution; ethyl alcohol; phosphate buffer solutions; compressed propellant gases, such as chlorofluorocarbons and hydrofluorocarbons; and other non-toxic compatible substances employed in pharmaceutical compositions.
Pharmaceutical compositions are typically prepared by thoroughly and intimately mixing or blending the active agent with a pharmaceutically acceptable carrier and one or more optional ingredients. The resulting uniformly blended mixture may then be shaped or loaded into tablets, capsules, pills, canisters, cartridges, dispensers, and the like, using conventional procedures and equipment.
In one embodiment, the pharmaceutical compositions are suitable for oral administration. One exemplary dosing regimen would be an oral dosage form administered once or twice daily. Suitable compositions for oral administration may be in the form of capsules, tablets, pills, lozenges, cachets, dragees, powders, granules; solutions or suspensions in an aqueous or non-aqueous liquid; oil-in-water or water-in-oil liquid emulsions; elixirs or syrups; and the like; each containing a predetermined amount of the active agent.
When intended for oral administration in a solid dosage form (i.e., as capsules, tablets, pills, and the like), the composition will typically comprise the active agent and one or more pharmaceutically acceptable carriers, such as sodium citrate or dicalcium phosphate. Solid dosage forms may also comprise: fillers or extenders, such as starches, microcrystalline cellulose, lactose, sucrose, glucose, mannitol, and/or silicic acid; binders, such as carboxymethylcellulose, alginates, gelatin, polyvinyl pyrrolidone, sucrose and/or acacia; humectants, such as glycerol; disintegrating agents, such as agar-agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, and/or sodium carbonate; solution retarding agents, such as paraffin; absorption accelerators, such as quaternary ammonium compounds; wetting agents, such as cetyl alcohol and/or glycerol monostearate; absorbents, such as kaolin and/or bentonite clay; lubricants, such as talc, calcium stearate, magnesium stearate, solid polyethylene glycols, sodium lauryl sulfate, and/or mixtures thereof; coloring agents; and buffering agents.
Release agents, wetting agents, coating agents, sweetening, flavoring and perfuming agents, preservatives and antioxidants may also be present in the pharmaceutical compositions. Exemplary coating agents for tablets, capsules, pills and like, include those used for enteric coatings, such as cellulose acetate phthalate, polyvinyl acetate phthalate, hydroxypropyl methylcellulose phthalate, methacrylic acid-methacrylic acid ester copolymers, cellulose acetate trimellitate, carboxymethyl ethyl cellulose, hydroxypropyl methyl cellulose acetate succinate, and the like. Examples of pharmaceutically acceptable antioxidants include: water-soluble antioxidants, such as ascorbic acid, cysteine hydrochloride, sodium bisulfate, sodium metabisulfate, sodium sulfite, and the like; oil-soluble antioxidants, such as ascorbyl palmitate, butylated hydroxyanisole, butylated hydroxytoluene, lecithin, propyl gallate, alpha-tocopherol, and the like; and metal-chelating agents, such as citric acid, ethylenediamine tetraacetic acid, sorbitol, tartaric acid, phosphoric acid, and the like.
Compositions may also be formulated to provide slow or controlled release of the active agent using, by way of example, hydroxypropyl methyl cellulose in varying proportions or other polymer matrices, liposomes and/or microspheres. In addition, the pharmaceutical compositions of the invention may contain opacifying agents and may be formulated so that they release the active agent only, or preferentially, in a certain portion of the gastrointestinal tract, optionally, in a delayed manner. Examples of embedding compositions which can be used include polymeric substances and waxes. The active agent can also be in micro-encapsulated form, if appropriate, with one or more of the above-described excipients.
Suitable liquid dosage forms for oral administration include, by way of illustration, pharmaceutically acceptable emulsions, microemulsions, solutions, suspensions, syrups and elixirs. Liquid dosage forms typically comprise the active agent and an inert diluent, such as, for example, water or other solvents, solubilizing agents and emulsifiers, such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, oils (e.g., cottonseed, groundnut, corn, germ, olive, castor and sesame oils), glycerol, tetrahydrofuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof. Suspensions may contain suspending agents such as, for example, ethoxylated isostearyl alcohols, polyoxyethylene sorbitol and sorbitan esters, microcrystalline cellulose, aluminium metahydroxide, bentonite, agar-agar and tragacanth, and mixtures thereof.
When intended for oral administration, the pharmaceutical compositions of the invention may be packaged in a unit dosage form. The term “unit dosage form” refers to a physically discrete unit suitable for dosing a patient, i.e., each unit containing a predetermined quantity of the active agent calculated to produce the desired therapeutic effect either alone or in combination with one or more additional units. For example, such unit dosage forms may be capsules, tablets, pills, and the like.
In another embodiment, the compositions of the invention are suitable for inhaled administration, and will typically be in the form of an aerosol or a powder. Such compositions are generally administered using well-known delivery devices, such as a nebulizer, dry powder, or metered-dose inhaler. Nebulizer devices produce a stream of high velocity air that causes the composition to spray as a mist that is carried into a patient's respiratory tract. An exemplary nebulizer formulation comprises the active agent dissolved in a carrier to form a solution, or micronized and combined with a carrier to form a suspension of micronized particles of respirable size. Dry powder inhalers administer the active agent as a free-flowing powder that is dispersed in a patient's air-stream during inspiration. An exemplary dry powder formulation comprises the active agent dry-blended with an excipient such as lactose, starch, mannitol, dextrose, polylactic acid, polylactide-co-glycolide, and combinations thereof. Metered-dose inhalers discharge a measured amount of the active agent using compressed propellant gas. An exemplary metered-dose formulation comprises a solution or suspension of the active agent in a liquefied propellant, such as a chlorofluorocarbon or hydrofluoroalkane. Optional components of such formulations include co-solvents, such as ethanol or pentane, and surfactants, such as sorbitan trioleate, oleic acid, lecithin, and glycerin. Such compositions are typically prepared by adding chilled or pressurized hydrofluoroalkane to a suitable container containing the active agent, ethanol (if present) and the surfactant (if present). To prepare a suspension, the active agent is micronized and then combined with the propellant. Alternatively, a suspension formulation can be prepared by spray drying a coating of surfactant on micronized particles of the active agent. The formulation is then loaded into an aerosol canister, which forms a portion of the inhaler.
Compounds of the invention can also be administered parenterally (e.g., by subcutaneous, intravenous, intramuscular, or intraperitoneal injection). For such administration, the active agent is provided in a sterile solution, suspension, or emulsion. Exemplary solvents for preparing such formulations include water, saline, low molecular weight alcohols such as propylene glycol, polyethylene glycol, oils, gelatin, fatty acid esters such as ethyl oleate, and the like. A typical parenteral formulation is a sterile pH 4-7 aqueous solution of the active agent. Parenteral formulations may also contain one or more solubilizers, stabilizers, preservatives, wetting agents, emulsifiers, and dispersing agents. These formulations may be rendered sterile by use of a sterile injectable medium, a sterilizing agent, filtration, irradiation, or heat.
Compounds of the invention can also be administered transdermally using known transdermal delivery systems and excipients. For example, the compound can be admixed with permeation enhancers, such as propylene glycol, polyethylene glycol monolaurate, azacycloalkan-2-ones, and the like, and incorporated into a patch or similar delivery system. Additional excipients including gelling agents, emulsifiers and buffers, may be used in such transdermal compositions if desired.
If desired, compounds of the invention may be administered in combination with one or more other therapeutic agents. Thus, in one embodiment, compositions of the invention may optionally contain other drugs that are co-administered with a compound of the invention. For example, the composition may further comprise one or more drugs (also referred to as “secondary agents(s)”) selected from the group of anti-Alzheimer's agents, anticonvulsants (antiepileptics), antidepressants, anti-Parkinson's agents, dual serotonin-norepinephrine reuptake inhibitors (SNRIs), non-steroidal anti-inflammatory agents (NSAIDs), norepinephrine reuptake inhibitors, opioid agonists (opioid analgesics), opioid antagonists, selective serotonin reuptake inhibitors, sodium channel blockers, sympatholytics, and combinations thereof. Numerous examples of such therapeutic agents are well known in the art, and examples are described herein. By combining a compound of the invention with a secondary agent, triple therapy can be achieved, i.e., serotonin reuptake inhibitory activity, norepinephrine reuptake inhibitory activity, and activity associated with the secondary agent (e.g., antidepressant activity), using only two active components. Since pharmaceutical compositions containing two active components are typically easier to formulate than compositions containing three active components, such two-component compositions provide a significant advantage over compositions containing three active components. Accordingly, in yet another aspect of the invention, a pharmaceutical composition comprises a compound of the invention, a second active agent, and a pharmaceutically acceptable carrier. Third, fourth, etc., active agents may also be included in the composition. In combination therapy, the amount of compound of the invention that is administered, as well as the amount of secondary agents, may be less than the amount typically administered in monotherapy.
A compound of the invention may be either physically mixed with the second active agent to form a composition containing both agents; or each agent may be present in separate and distinct compositions which are administered to the patient simultaneously or sequentially. For example, a compound of the invention can be combined with a second active agent using conventional procedures and equipment to form a combination of active agents comprising a compound of the invention and a second active agent. Additionally, the active agents may be combined with a pharmaceutically acceptable carrier to form a pharmaceutical composition comprising a compound of the invention, a second active agent and a pharmaceutically acceptable carrier. In this embodiment, the components of the composition are typically mixed or blended to create a physical mixture. The physical mixture is then administered in a therapeutically effective amount using any of the routes described herein.
Alternatively, the active agents may remain separate and distinct before administration to the patient. In this embodiment, the agents are not physically mixed together before administration but are administered simultaneously or at separate times as separate compositions. Such compositions can be packaged separately or may be packaged together in a kit. When administered at separate times, the secondary agent will typically be administered less than 24 hours after administration of the compound of the invention, ranging anywhere from concurrent with administration of the compound of the invention to about 24 hours post-dose. This is also referred to as sequential administration. Thus, a compound of the invention can be orally administered simultaneously or sequentially with another active agent using two tablets, with one tablet for each active agent, where sequential may mean being administered immediately after administration of the compound of the invention or at some predetermined time later (e.g., one hour later or three hours later). Alternatively, the combination may be administered by different routes of administration, i.e., one orally and the other by inhalation.
In one embodiment, the kit comprises a first dosage form comprising a compound of the invention and at least one additional dosage form comprising one or more of the secondary agents set forth herein, in quantities sufficient to carry out the methods of the invention. The first dosage form and the second (or third, etc.) dosage form together comprise a therapeutically effective amount of active agents for the treatment or prevention of a disease or medical condition in a patient.
Secondary agent(s), when included, are present in a therapeutically effective amount, i.e., are typically administered in an amount that produces a therapeutically beneficial effect when co-administered with a compound of the invention. The secondary agent can be in the form of a pharmaceutically acceptable salt, solvate, optically pure stereoisomer, and so forth. Thus, secondary agents listed below are intended to include all such forms, and are commercially available or can be prepared using conventional procedures and reagents.
Representative anti-Alzheimer's agents include, but are not limited to: donepezil, galantamine, memantine, rivastigmine, selegiline, tacrine, and combinations thereof.
Representative anticonvulsants (antiepileptics) include, but are not limited to: acetazolamide, albutoin, 4-amino-3-hydroxybutyric acid, beclamide, carbamazepine, cinromide, clomethiazole, clonazepam, diazepam, dimethadione, eterobarb, ethadione, ethosuximide, ethotoin, felbamate, fosphenyloin, gabapentin, lacosamide, lamotrigine, lorazepam, magnesium bromide, magnesium sulfate, mephenyloin, mephobarbital, methsuximide, midazolam, nitrazepam, oxazepam, oxcarbazepine, paramethadione, phenacemide, pheneturide, phenobarbital, phensuximide, phenyloin, potassium bromide, pregabalin, primidone, progabide, sodium bromide, sodium valproate, sulthiame, tiagabine, topiramate, trimethadione, valproic acid, valpromide, vigabatrin, zonisamide, and combinations thereof. In a particular embodiment, the anticonvulsant is selected from carbamazepine, gabapentin, pregabalin, and combinations thereof.
Representative antidepressants include, but are not limited to: adinazolam, amitriptyline, clomipramine, desipramine, dothiepin (e.g., dothiepin hydrochloride), doxepin, imipramine, lofepramine, mirtazapine, nortriptyline, protriptyline, trimipramine, venlafaxine, zimelidine, and combinations thereof.
Representative anti-Parkinson's agents include, but are not limited to: amantadine, apomorphine, benztropine, bromocriptine, carbidopa, diphenhydramine, entacapone, levodopa, pergolide, pramipexole, ropinirole, selegiline, tolcapone, trihexyphenidyl, and combinations thereof.
Representative dual serotonin-norepinephrine reuptake inhibitors (SNRIs) include, but are not limited to: bicifadine, desvenlafaxine, duloxetine, milnacipran, nefazodone, venlafaxine, and combinations thereof.
Representative non-steroidal anti-inflammatory agents (NSAIDs) include, but are not limited to: acemetacin, acetaminophen, acetyl salicylic acid, alclofenac, alminoprofen, amfenac, amiprilose, amoxiprin, anirolac, apazone, azapropazone, benorilate, benoxaprofen, bezpiperylon, broperamole, bucloxic acid, carprofen, clidanac, diclofenac, diflunisal, diftalone, enolicam, etodolac, etoricoxib, fenbufen, fenclofenac, fenclozic acid, fenoprofen, fentiazac, feprazone, flufenamic acid, flufenisal, fluprofen, flurbiprofen, furofenac, ibufenac, ibuprofen, indomethacin, indoprofen, isoxepac, isoxicam, ketoprofen, ketorolac, lofemizole, lornoxicam, meclofenamate, meclofenamic acid, mefenamic acid, meloxicam, mesalamine, miroprofen, mofebutazone, nabumetone, naproxen, niflumic acid, nimesulide, nitroflurbiprofen, olsalazine, oxaprozin, oxpinac, oxyphenbutazone, phenylbutazone, piroxicam, pirprofen, pranoprofen, salsalate, sudoxicam, sulfasalazine, sulindac, suprofen, tenoxicam, tiopinac, tiaprofenic acid, tioxaprofen, tolfenamic acid, tolmetin, triflumidate, zidometacin, zomepirac, and combinations thereof. In a particular embodiment, the NSAID is selected from etodolac, flurbiprofen, ibuprofen, indomethacin, ketoprofen, ketorolac, meloxicam, naproxen, oxaprozin, piroxicam, and combinations thereof. In a particular embodiment, the NSAID is selected from ibuprofen, indomethacin, nabumetone, naproxen (for example, naproxen sodium), and combinations thereof.
Representative muscle relaxants include, but are not limited to: carisoprodol, chlorzoxazone, cyclobenzaprine, diflunisal, metaxalone, methocarbamol, and combinations thereof.
Representative norepinephrine reuptake inhibitors include, but are not limited to: atomoxetine, buproprion and the buproprion metabolite hydroxybuproprion, maprotiline, reboxetine (for example, (S,S)-reboxetine), viloxazine, and combinations thereof. In a particular embodiment, the norepinephrine reuptake inhibitor is selected from atomoxetine, reboxetine, and combinations thereof.
Representative opioid agonists (opioid analgesics) and antagonists include, but are not limited to: buprenorphine, butorphanol, codeine, dihydrocodeine, fentanyl, hydrocodone, hydromorphone, levallorphan, levorphanol, meperidine, methadone, morphine, nalbuphine, nalmefene, nalorphine, naloxone, naltrexone, nalorphine, oxycodone, oxymorphone, pentazocine, propoxyphene, tramadol, and combinations thereof. In certain embodiments, the opioid agonist is selected from codeine, dihydrocodeine, hydrocodone, hydromorphone, morphine, oxycodone, oxymorphone, tramadol, and combinations thereof.
Representative selective serotonin reuptake inhibitors (SSRIs) include, but are not limited to: citalopram and the citalopram metabolite desmethylcitalopram, dapoxetine, escitalopram (e.g., escitalopram oxalate), fluoxetine and the fluoxetine desmethyl metabolite norfluoxetine, fluvoxamine (e.g., fluvoxamine maleate), paroxetine, sertraline and the sertraline metabolite demethylsertraline, and combinations thereof. In certain embodiments, the SSRI is selected from citalopram, paroxetine, sertraline, and combinations thereof.
Representative sodium channel blockers include, but are not limited to: carbamazepine, fosphenyloin, lamotrignine, lidocaine, mexiletine, oxcarbazepine, phenyloin, and combinations thereof.
Representative sympatholytics include, but are not limited to: atenolol, clonidine, doxazosin, guanethidine, guanfacine, modafinil, phentolamine, prazosin, reserpine, tolazoline (e.g., tolazoline hydrochloride), tamsulosin, and combinations thereof.
The following formulations illustrate representative pharmaceutical compositions of the present invention:

Exemplary Hard Gelatin Capsules for Oral Administration

A compound of the invention (50 g), spray-dried lactose (440 g) and magnesium stearate (10 g) are thoroughly blended. The resulting composition is then loaded into hard gelatin capsules (500 mg of composition per capsule).
Alternately, a compound of the invention (20 mg) is thoroughly blended with starch (89 mg), microcrystalline cellulose (89 mg) and magnesium stearate (2 mg). The mixture is then passed through a No. 45 mesh U.S. sieve and loaded into a hard gelatin capsule (200 mg of composition per capsule).

Exemplary Gelatin Capsule Formulation for Oral Administration

A compound of the invention (100 mg) is thoroughly blended with polyoxyethylene sorbitan monooleate (50 mg) and starch powder (250 mg). The mixture is then loaded into a gelatin capsule (400 mg of composition per capsule).
Alternately, a compound of the invention (40 mg) is thoroughly blended with microcrystalline cellulose (Avicel PH 103; 259.2 mg) and magnesium stearate (0.8 mg). The mixture is then loaded into a gelatin capsule (Size #1, White, Opaque) (300 mg of composition per capsule).

Exemplary Tablet Formulation for Oral Administration

A compound of the invention (10 mg), starch (45 mg) and microcrystalline cellulose (35 mg) are passed through a No. 20 mesh U.S. sieve and mixed thoroughly. The granules so produced are dried at 50-60° C. and passed through a No. 16 mesh U.S. sieve.
A solution of polyvinylpyrrolidone (4 mg as a 10% solution in sterile water) is mixed with sodium carboxymethyl starch (4.5 mg), magnesium stearate (0.5 mg), and talc (1 mg), and this mixture is then passed through a No. 16 mesh U.S. sieve. The sodium carboxymethyl starch, magnesium stearate and talc are then added to the granules. After mixing, the mixture is compressed on a tablet machine to afford a tablet weighing 100 mg.
Alternately, a compound of the invention (250 mg) is thoroughly blended with microcrystalline cellulose (400 mg), silicon dioxide fumed (10 mg), and stearic acid (5 mg). The mixture is then compressed to form tablets (665 mg of composition per tablet).
Alternately, a compound of the invention (400 mg) is thoroughly blended with cornstarch (50 mg), croscarmellose sodium (25 mg), lactose (120 mg), and magnesium stearate (5 mg). The mixture is then compressed to form a single-scored tablet (600 mg of compositions per tablet).

Exemplary Suspension Formulation for Oral Administration

The following ingredients are mixed to form a suspension containing 100 mg of active agent per 10 mL of suspension:


	Ingredients	Amount

Compound of the invention	1.0	g
Fumaric acid	0.5	g
Sodium chloride	2.0	g
Methyl paraben	0.15	g
Propyl paraben	0.05	g
Granulated sugar	25.5	g
Sorbitol (70% solution)	12.85	g
Veegum ® K (magnesium	1.0	g
aluminum silicate)
Flavoring	0.035	mL
Colorings	0.5	mg
Distilled water	q.s. to 100	mL

Exemplary Injectable Formulation for Administration by Injection

A compound of the invention (0.2 g) is blended with 0.4 M sodium acetate buffer solution (2.0 mL). The pH of the resulting solution is adjusted to pH 4 using 0.5 N aqueous hydrochloric acid or 0.5 N aqueous sodium hydroxide, as necessary, and then sufficient water for injection is added to provide a total volume of 20 mL. The mixture is then filtered through a sterile filter (0.22 micron) to provide a sterile solution suitable for administration by injection.

Exemplary Compositions for Administration by Inhalation

A compound of the invention (0.2 mg) is micronized and then blended with lactose (25 mg). This blended mixture is then loaded into a gelatin inhalation cartridge. The contents of the cartridge are administered using a dry powder inhaler, for example.
Alternately, a micronized compound of the invention (10 g) is dispersed in a solution prepared by dissolving lecithin (0.2 g) in demineralized water (200 mL). The resulting suspension is spray dried and then micronized to form a micronized composition comprising particles having a mean diameter less than about 1.5 μm. The micronized composition is then loaded into metered-dose inhaler cartridges containing pressurized 1,1,1,2-tetrafluoroethane in an amount sufficient to provide about 10 μg to about 500 μg of the compound of the invention per dose when administered by the inhaler.
Alternately, a compound of the invention (25 mg) is dissolved in citrate buffered (pH 5) isotonic saline (125 mL). The mixture is stirred and sonicated until the compound is dissolved. The pH of the solution is checked and adjusted, if necessary, to pH 5 by slowly adding aqueous 1N sodium hydroxide. The solution is administered using a nebulizer device that provides about 10 μg to about 500 μg of the compound of the invention per dose.

EXAMPLES

The following Preparations and Examples are provided to illustrate specific embodiments of the invention. These specific embodiments, however, are not intended to limit the scope of the invention in any way unless specifically indicated.
The following abbreviations have the following meanings unless otherwise indicated and any other abbreviations used herein and not defined have their standard meaning:

- AcOH acetic acid
- BSA bovine serum albumin
- DABCO 1,4-diazabicyclo[2.2.2]octane or triethylenediamine
- DCM dichloromethane (i.e., methylene chloride)
- DIAD diisopropyl azodicarboxylate
- DMAP N,N-dimethylpyridin-4-amine
- DMEM Dulbecco's Modified Eagle's Medium
- DPPF 1,1′-bis(Diphenylphosphino)ferrocene
- DMSO dimethylsulfoxide
- EDC N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride
- EDTA ethylenediaminetetraacetic acid
- EtOAc ethyl acetate
- EtOH ethanol
- FBS fetal bovine serum
- hDAT human dopamine transporter
- HEPES 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
- hNET human norepinephrine transporter
- hSERT human serotonin transporter
- 5-HT 5-hydroxytryptamine
- Me methyl
- MeCN acetonitrile (CH₃CN)
- MeOH methanol
- Pd(PPh₃)₂Cl₂dichlorobis(triphenylphosphine)palladium (II)
- PBS phosphate buffered saline
- PE petroleum ether
- PPh₃triphenylphosphine
- Tf triflate or trifluoromethanesulfonate (—S(O)₂—CF₃)
- TFA trifluoroacetic acid
- THF tetrahydrofuran
- TsCl p-toluenesulfonyl chloride or 4-methylbenzenesulfonyl chloride

Any other abbreviations used herein but not defined have their standard, generally accepted meaning. Unless noted otherwise, all materials, such as reagents, starting materials and solvents, were purchased from commercial suppliers (such as Sigma-Aldrich, Fluka Riedel-de Haën, and the like) and were used without further purification.

Preparation 1

4-(4,4,5,5-Tetramethyl-[1,3,2]dioxaborolan-2-yl)-3,6-dihydro-2H-pyridine-1-carboxylic Acid t-Butyl Ester

Triethylamine (280 mL, 2.0 mol, 2.5 eq.) was added, in several batches, to a solution of phenylamine (75.0 g, 805.3 mmol, 1.0 eq.) in DCM (550 mL) under nitrogen. This solution was cooled to −78° C. then trifluoromethanesulfonic anhydride (270 mL, 1.6 mol, 2.0 eq.) was added. The resulting solution was stirred for 2 hours while the temperature was maintained at −78° C. in an EtOH/liquid N₂bath. The resulting solution was stirred while the temperature was allowed to increase to room temperature. The mixture was stirred overnight at room temperature, then washed with 10% HCl (3×300 mL). The organic layer was then washed with saturated aqueous NaCl (3×300 mL). The combined organic layers were dried over MgSO₄and concentrated under vacuum. The crude product was purified by re-crystallization from MeOH to yield N-phenyl-bis(trifluoromethanesulfonimide) (140 g) as a pink solid.
n-Butyllithium (188 mL) was added dropwise to a solution of diisopropylamine (47.6 g, 470.3 mmol, 1.2 eq.) in THF (550 mL), at −78° C. under nitrogen. The resulting solution was stirred for one hour at −78° C. This was followed by the addition of a solution of 4-oxopiperidine-1-carboxylic acid t-butyl ester (84.4 g, 423.6 mmol, 1.1 eq.) in THF (400 mL), which was added dropwise with stirring at −78° C. The resulting solution was stirred for two hours at −60 to −70° C. A solution of N-phenyl-bis(trifluoromethane-sulfonimide) (140.0 g, 391.98 mmol, 1.0 eq.) in THF (300 mL) was then added dropwise with stirring at −78° C. The resulting solution was stirred for two hours at −60 to −70° C., then continuously stirred while the temperature was allowed to reach room temperature. The mixture was then stirred overnight at room temperature and concentrated under vacuum. The residue was dissolved in THF (300 mL) and washed with 5% NaHCO₃(2×400 mL) and 15% NaHSO₄(2×400 mL). The combined organic layers were washed with Na₂CO₃(2×100 mL) and saturated aqueous NaCl (200 mL), then dried over anhydrous Na₂SO₄. The residue was applied onto a silica gel column and eluted with EtOAc/PE (1:10) to yield t-butyl 4-(trifluoromethylsulfonyloxy)-5,6-dihydropyridine-1(2H)-carboxylate (92.0 g) as yellow oil.
DPPF (6.0 g, 10.8 mmol, 0.05 eq.) was added to a solution of 4,4,5,5,4′,4′,5′,5′-cctamethyl-[2,2′]bi[[1,3,2]dioxaborolanyl] (72.8 g, 286.7 mmol, 1.2 eq.) in 1,4-dioxane (1000 mL), under nitrogen. PdCl₂/DPPF/CHCl₃(6.7 g, 7.9 mmol, 0.03 eq.) was then added, followed by the addition of potassium acetate (76.0 g, 774.4 mmol, 3.3 eq.). To the mixture was added t-butyl 4-(trifluoromethylsulfonyloxy)-5,6-dihydropyridine-1(2H)-carboxylate (78.0 g, 235.4 mmol, 1.0 eq.). The resulting solution was stirred overnight while the temperature was maintained at 80° C. in an oil bath. The mixture was then cooled to 20° C. with a water/ice bath. The reaction mixture was then filtered and the filter cake was washed with EtOAc and the filtrate was washed with saturated aqueous NaCl (3×500 mL). The organic layer was dried over anhydrous Na₂SO₄and concentrated under vacuum. The residue was applied onto a silica gel column and eluted with EtOAc/PE (1:20) to yield the title compound (65.0 g) as a white solid.

Preparation 2

2-Hydroxymethyl-3′,4′,5′,6′-tetrahydro-2′H-[3,4′]-bipyridinyl-1′-carboxylic Acid t-Butyl Ester

H₂SO₄(1.2 mL, 23.4 mmol, 1.0 eq.) was added to a solution of 3-bromopyridine-2-carboxylic acid (4.7 g, 23.4 mmol, 1.0 eq.) in MeOH (50 mL). The resulting solution was stirred for 14 hours while the temperature was maintained at reflux in an oil bath. The mixture was cooled to room temperature and concentrated under vacuum. The residue was dissolved in EtOAc (50 mL) and the pH was adjusted to 10 with Na₂CO₃(5 mol/L). The resulting solution was extracted with EtOAc (100 mL). The organic layer was washed with water (1×100 mL) and saturated aqueous NaCl (2×100 mL), dried over anhydrous Na₂SO₄and concentrated under vacuum. The residue was applied onto a silica gel column and eluted with EtOAc/PE (1:20) to yield 3-bromopyridine-2-carboxylic acid methyl ester (4.0 g) as a yellow liquid.
3-Bromopyridine-2-carboxylic acid methyl ester (4.0 g, 18.6 mmol, 1.0 eq.) was combined with a solution of 4-(4,4,5,5-tetramethyl-[1,3,2]dioxaborolan-2-yl)-3,6-dihydro-2H-pyridine-1-carboxylic acid t-butyl ester (5.8 g, 18.6 mmol, 1.0 eq.) in THF (100 mL), under nitrogen. A solution of Na₂CO₃(7.9 g, 74.5 mmol, 4.0 eq.) in water (37 mL) was added, followed by the addition of PdCl₂(PPh₃)₂(650 mg, 930 μmol, 0.05 eq.). The resulting solution was stirred for 14 hours while the temperature was maintained at reflux in an oil bath. The resulting solution was diluted with EtOAc (100 mL), then washed with saturated aqueous NaCl (2×100 mL). The organic layer was dried over anhydrous Na₂SO₄and concentrated under vacuum. The residue was applied onto a silica gel column and eluted with EtOAc/PE (1:20) to yield 3′,6′-dihydro-2′H-[3,4′]bipyridinyl-2,1′-dicarboxylic acid 1′-t-butyl ester 2-methyl ester (3.8 g) as colorless oil.
H-NMR: (300 MHz, CDCl₃, ppm) 8.67-8.64 (1H, dd, J=4.5 Hz), 7.60-7.63 (1H, dd, J=7.8 Hz), 7.42-7.46 (1H, t, J=7.8 Hz), 5.62 (1H, t), 4.06-4.07 (2H, d, J=2.4 Hz), 3.96 (3H, s), 3.67-3.69 (2H, t, J=12.1 Hz), 2.40 (2H, m), 1.52 (9H, s).
3′,6′-dihydro-2′H-[3,4′]bipyridinyl-2,1′-dicarboxylic acid F-t-butyl ester 2-methyl ester (3.8 g, 11.0 mmol, 1.0 eq.) was dissolved in MeOH (100 mL) and the flask was flushed and maintained under hydrogen at 1 atm. To the mixture was added Pd(OH)₂/C (1.7 g, 2.2 mmol, 0.2 eq.). The resulting solution was stirred overnight at room temperature. The solids were filtered, and the remaining mixture was concentrated under vacuum. The residue was applied onto a silica gel column and eluted with EtOAc/PE (1:1) to yield 3′,4′,5′,6′-tetrahydro-2′H-[3,4′]bipyridinyl-2,1′-dicarboxylic acid F-t-butyl ester 2-methyl ester (2.2 g) as a white solid.
H-NMR: (300 MHz, CDCl₃, ppm) 8.59-8.58 (1H, dd, J=4.5 Hz), 7.72-7.75 (1H, dd, J=8.1 Hz), 7.41-7.45 (1H, t, J=8.1 Hz), 4.26 (2H, m), 4.01 (3H, s), 3.41-3.50 (1H, t), 2.82-2.90 (2H, t, J=24.3 Hz), 1.84-1.89 (2H, t, J=12.9 Hz), 1.54-1.67 (2H, t), 1.47 (9H, s).
A solution of 3′,4′,5′,6′-tetrahydro-2′H-[3,4′]bipyridinyl-2,1′-dicarboxylic acid 1′-t-butyl ester 2-methyl ester (1.9 g, 5.9 mmol, 1.0 eq.) in THF (50 mL) was maintained under nitrogen. To the mixture was added NaBH₄(1.1 g, 29.5 mmol, 5.0 eq.), followed by the dropwise addition of MeOH (15 mL), while stirring at 0° C. The resulting solution was stirred for 30 minutes at room temperature. The resulting solution was diluted with EtOAc (150 mL). The resulting mixture was washed with a saturated solution of NH₄Cl (1×100 mL), then with saturated aqueous NaCl (2×100 mL). The organic layer was dried over anhydrous Na₂SO₄and concentrated under vacuum. The residue was applied onto a silica gel column and eluted with EtOAc/PE (1:1) to yield the title compound (860 mg) as a white solid. MS m/z: [M+H]⁺ calcd for C₁₆H₂₅N₂O₃, 293. found 293.
H-NMR: (300 MHz, CDCl₃, ppm) 8.46-8.47 (1H, d, J=3.6 Hz), 7.57-7.60 (1H, d, J=7.8 Hz), 7.25-7.29 (1H, t, J=7.8 Hz), 4.87 (2H, s), 4.28 (2H, m), 2.80-2.88 (2H, m), 2.63-2.69 (1H, t, J=12 Hz), 1.68-1.78 (2H, m), 1.60-1.65 (2H, t, J=12.3 Hz), 1.51 (9H, s).

Example 1

2-(2,4,6-Trifluorophenoxymethyl)-1′,2′,3′,4′,5′,6′-hexahydro[3,4′]bipyridinyl

PPh₃(28.9 mg, 0.1 mmol, 1.1 eq.) and 2-hydroxymethyl-3′,4′,5′,6′-tetrahydro-2′H-[3,4′]bipyridinyl-1′-carboxylic acid t-butyl ester (29.2 mg, 0.1 mmol, 1.0 eq.) were dissolved in toluene (533 μL). 2,4,6-Trifluorophenol (14.8 mg, 0.1 mmol, 1.0 eq.) was added, followed by the addition of DIAD (23.6 μL, 0.1 mmol, 1.2 eq.) in toluene (5 mL). The mixture was heated at 80° C. for 3 hours, then concentrated using an evaporator. The material was then dissolved in 1.25M HCl in EtOH (1.5 mL), and stirred at room temperature overnight. The material was again concentrated using an evaporator, then dissolved in 1:1 AcOH:H₂O, filtered, and purified by preparative HPLC to yield the title compound as a TFA salt (37.9 mg, 100% purity). MS m/z: [M+H]⁺ calcd for C₁₇H₁₇F₃N₂O, 323.13. found 323.0.

Example 2

Following the procedures described in the Schemes and/or the examples above, and substituting the appropriate starting materials and reagents, compounds 2-1 to 2-9, having formula Ia′, were also prepared:


	(Ia′)

MS m/z: [M + H]⁺

Ex.	R²	R³	R⁴	R⁵	R⁶	Formula	calcd	found

1	H	H	H	H	H	C₁₇H₂₀N₂O	269.16	269.2
2	F	H	H	H	H	C₁₇H₁₉FN₂O	287.15	287.2
3	H	Cl	H	H	H	C₁₇H₁₉ClN₂O	303.12	303.0
4	F	H	H	H	F	C₁₇H₁₈F₂N₂O	305.14	305.0
5	F	H	H	H	Cl	C₁₇H₁₈ClFN₂O	321.11	321.0
6	Cl	H	H	H	Cl	C₁₇H₁₈Cl₂N₂O	337.08	337.0
7	F	F	H	H	F	C₁₇H₁₇F₃N₂O	323.13	323.0
8	F	H	Cl	H	F	C₁₇H₁₇ClF₂N₂O	339.10	339.0
9	F	H	H	F	Cl	C₁₇H₁₇ClF₂N₂O	339.10	339.0

Example 3

Following the procedures described in the Schemes and/or the examples above, and substituting the appropriate starting materials and reagents, compounds 3-1 to 3-47, having formula Ia″, can also be prepared:


	(Ia″)

Ex.	R^1a	R^1b	R^1c	R²	R³	R⁴	R⁵	R⁶

1	H	H	H	F	H	H	H	Br
2	H	H	H	F	Cl	H	H	F
3	H	H	H	F	H	Br	H	Cl
4	H	H	H	F	H	F	H	Cl
5	H	H	H	F	Me	H	H	F
6	F	H	H	F	H	H	H	H
7	H	F	H	F	H	H	H	H
8	H	H	F	F	H	H	H	H
9	F	H	H	F	H	H	H	F
10	H	F	H	F	H	H	H	F
11	H	H	F	F	H	H	H	F
12	F	H	H	F	H	H	H	Cl
13	H	F	H	F	H	H	H	Cl
14	H	H	F	F	H	H	H	Cl
15	F	H	H	F	F	H	H	F
16	H	F	H	F	F	H	H	F
17	H	H	F	F	F	H	H	F
18	F	H	H	F	H	F	H	F
19	H	F	H	F	H	F	H	F
20	H	H	F	F	H	F	H	F
21	F	H	H	F	H	H	F	Cl
22	H	F	H	F	H	H	F	Cl
23	H	H	F	F	H	H	F	Cl
24	—CF₃	H	H	F	H	H	H	Cl
25	H	—CF₃	H	F	H	H	H	Cl
26	H	H	—CF₃	F	H	H	H	Cl
27	—CF₃	H	H	F	F	H	H	F
28	H	—CF₃	H	F	F	H	H	F
29	H	H	—CF₃	F	F	H	H	F
30	—CF₃	H	H	F	H	F	H	F
31	H	—CF₃	H	F	H	F	H	F
32	H	H	—CF₃	F	H	F	H	F
33	F	F	H	F	H	H	H	F
34	F	H	F	F	H	H	H	F
35	H	F	F	F	H	H	H	F
36	F	F	H	F	H	H	H	Cl
37	F	H	F	F	H	H	H	Cl
38	H	F	F	F	H	H	H	Cl
39	F	F	H	F	H	F	H	F
40	F	H	F	F	H	F	H	F
41	H	F	F	F	H	F	H	F
42	F	F	H	F	F	H	H	F
43	F	H	F	F	F	H	H	F
44	H	F	F	F	F	H	H	F
45	F	F	H	F	H	H	F	Cl
46	F	H	F	F	H	H	F	Cl
47	H	F	F	F	H	H	F	Cl

Example 4

Following the procedures described in the Schemes and/or the examples above (for example, using the Ullmann reaction), and substituting the appropriate starting materials and reagents, compounds 4-1 to 4-15, having formula Ia′″, can also be prepared:


		(Ia′′′)

Ex.	R²	R³	R⁴	R⁵	R⁶

1	F	H	F	H	F
2	H	H	H	H	H
3	F	H	H	H	H
4	H	Cl	H	H	H
5	F	H	H	H	F
6	F	H	H	H	Cl
7	Cl	H	H	H	Cl
8	F	F	H	H	F
9	F	H	Cl	H	F
10	F	H	H	F	Cl
11	F	H	H	H	Br
12	F	Cl	H	H	F
13	F	H	Br	H	Cl
14	F	H	F	H	Cl
15	F	Me	H	H	F

Preparation 3

3′-Hydroxymethyl-3,4,5,6-tetrahydro-2H-[4,4′]bipyridinyl-1-carboxylic Acid t-Butyl Ester

Butyllithium (82.0 mL, 205.6 mmol, 2.0 eq.) was added dropwise to a solution of diisopropylamine (20.8 g, 205.6 mmol, 2.0 eq.) in THF (100 mL) at −78° C., under nitrogen. The resulting solution was stirred for 1 hour at approximately −60 to −50° C. The resulting solution was added dropwise to a solution of 4-bromopyridine hydrochloride (20.0 g, 102.9 mmol, 1.0 eq.) in THF (100 mL) at −78° C. The resulting solution was stirred for 2 hours. Carbon dioxide gas was introduced into the reaction vessel for 30 minutes. The resulting solution was stirred overnight at room temperature. The reaction was then quenched with water (100 mL). The resulting solution was extracted with EtOAc (3×200 mL) and the aqueous layers were combined. The pH of the solution was adjusted to 3-4 with 1N HCl to yield 4-bromonicotinic acid (16.8 g) as a white solid. ES m/z: [M+H]⁺202.
H-NMR: (300 MHz, DMSO, ppm): 8.88 (1H, s), 8.53 (1H, d, J=5.4 Hz), 7.84 (1H, d, J=5.4 Hz).
To a solution of 4-bromonicotinic acid (16.8 g, 83.2 mmol, 1.0 eq.) in DCM (250 mL) at 0° C. was added DMAP (1.0 g, 8.2 mmol, 0.1 eq.). MeOH (2.7 g, 83.7 mmol, 1.0 eq.) was added dropwise with stirring at 0° C. EDC (16.8 g, 87.6 mmol, 1.1 eq.) was then added. The resulting solution was stirred for 3 hours at 0° C. The reaction was then quenched with water (100 mL). The resulting solution was extracted with DCM. The organic layer was washed with saturated aqueous NaCl (3×100 mL), dried over anhydrous Na₂SO₄and concentrated under vacuum to yield 4-bromonicotinic acid methyl ester (8.2 g) as a yellow oil.
A solution of PdCl₂(PPh₃)₂(1.3 g, 1.9 mmol, 0.05 eq.) in THF (250 mL) was stirred for 10 minutes under nitrogen. 4-(4,4,5,5-Tetramethyl-[1,3,2]dioxaborolan-2-yl)-3,6-dihydro-2H-pyridine-1-carboxylic acid t-butyl ester (11.7 g, 37.8 mmol, 1.0 eq.) was added and the resulting solution was stirred for another 10 minutes. 4-Bromonicotinic acid methyl ester (8.2 g, 38.0 mmol, 1.0 eq.) in THF (100 mL) and NaHCO₃(16.1 g, 151.9 mmol, 4.0 eq.) in H₂O (76 mL) were added, and the resulting solution was stirred for 6 hours while the temperature was maintained at 72° C. in an oil bath. The mixture was cooled to room temperature and the reaction was then quenched with water (50 mL). The resulting solution was extracted with EtOAc (150 mL). The organic layer was washed with saturated aqueous NaCl (3×200 mL), dried over anhydrous Na₂SO₄and concentrated under vacuum. The residue was applied onto a silica gel column and eluted with EtOAc/PE (1:5) to yield 3,6-dihydro-2H-[4,4′]bipyridinyl-1,3′-dicarboxylic acid 1-t-butyl ester 3′-methyl ester (8.7 g) as a white solid. ES m/z: [M+H]⁺319.
H-NMR: (300 MHz, CDCl₃, ppm): 9.05 (1H, s), 8.67 (1H, d, J=5.1 Hz), 7.17 (1H, d, J=5.1 Hz), 5.64 (1H, s), 4.07 (2H, m), 3.92 (3H, s), 3.66 (2H, m), 2.35 (2H, s), 1.52 (9H, s).
A solution of Pd(OH)₂/C (5.2 g, 7.4 mmol, 0.3 eq., 20%) in EtOAc (100 mL) was stirred for 10 minutes at room temperature under nitrogen. 3,6-Dihydro-2H-[4,4′]bipyridinyl-1,3′-dicarboxylic acid 1-t-butyl ester 3′-methyl ester (8.7 g, 27.3 mmol, 1.0 eq.) was added. Hydrogen gas was introduced into the reaction vessel (at 1 atm) and the solution was stirred for 5 hours at room temperature. The solids were filtered and the resulting mixture was concentrated under vacuum. The residue was applied onto a silica gel column and eluted with EtOAc/PE (1:5) to yield 3,4,5,6-tetrahydro-2H-[4,4′]bipyridinyl-1,3′-dicarboxylic acid 1-t-butyl ester 3′-methyl ester (6.7 g) as a colorless oil.
H-NMR: (300 MHz, CDCl₃, ppm): 9.04 (1H, s), 8.66 (1H, d, J=5.1 Hz), 7.3 (1H, d, J=5.1 Hz), 4.14 (2H, m), 3.96 (3H, s), 3.67 (1H, m), 2.86 (2H, m), 2.08 (2H, m), 1.85 (2H, m), 1.29 (9H, m).
A solution of methyl 4-(1-(tert-butoxycarbonyl)piperidin-4-yl)nicotinate (2.6 g, 8.1 mmol, 1.0 eq.) in THF (50 mL) was added dropwise to a solution of lithium aluminium hydride (617 mg, 16.3 mmol, 2.0 eq.) in THF (100 mL) at 0° C. under nitrogen. The resulting solution was stirred for 0.5 hours while the temperature was maintained at 0° C. in an ice/salt bath. The reaction was then quenched with water (0.6 mL) and 15% NaOH (1.8 mL). The mixture was stirred for 10 minutes. The solids were filtered and washed with EtOAc (30 mL). The filtrate was dried over anhydrous Na₂SO₄and concentrated under vacuum. The residue was applied onto a silica gel column and eluted with EtOAc/MeOH (10:1) to yield the title compound (1.6 g) as a white solid. ES m/z: [M+H]⁺293.
H-NMR: (300 MHz, CDCl₃, ppm): 8.47 (1H, s), 8.42 (1H, d, J=5.1 Hz), 7.38 (1H, d, J=5.4 Hz), 4.75 (2H, s), 4.25 (2H, m), 3.17 (1H, m), 2.92 (2H, m), 1.76 (2H, m), 1.63 (2H, m), 1.50 (9H, s).

Example 5

3′(2,6-Difluorophenoxymethyl)-1,2,3,4,5,6-hexahydro-[4,4′]bipyridinyl

PPh₃(28.9 mg, 0.1 mmol, 1.1 eq.) and 3′-hydroxymethyl-3,4,5,6-tetrahydro-2H-[4,4′]bipyridinyl-1-carboxylic acid t-butyl ester (29.2 mg, 0.1 mmol, 1.0 eq.) were dissolved in toluene (533 μL). 2,6-Difluorophenol (13.0 mg, 0.1 mmol, 1.0 eq.) was added, followed by the addition of DIAD (23.6 μL, 0.1 mmol, 1.2 eq.) in toluene (5 mL). The mixture was heated at 80° C. for 4 hours, then concentrated using an evaporator. The material was then dissolved in 1.25M HCl in EtOH (1.0 mL), and stirred at room temperature overnight. The material was again concentrated using an evaporator, then dissolved in 1:1 AcOH:H₂O, filtered, and purified by preparative HPLC to yield the title compound as a TFA salt (13.8 mg, 100% purity). MS m/z: [M+H]⁺ calcd for C₁₇H₁₈F₂N₂O, 305.14. found 305.2.

Example 6

Following the procedures described in the Schemes and/or the examples above, and substituting the appropriate starting materials and reagents, compounds 6-1 to 6-9, having formula Ib′, were also prepared:


	(Ib′)

MS m/z: [M + H]⁺

Ex.	R²	R³	R⁴	R⁵	R⁶	Formula	calcd	found

1	H	H	H	H	H	C₁₇H₂₀N₂O	269.16	269.2
2	F	H	H	H	H	C₁₇H₁₉FN₂O	287.15	287.2
3	H	Cl	H	H	H	C₁₇H₁₉ClN₂O	303.12	303.0
4	F	H	H	H	Cl	C₁₇H₁₈ClFN₂O	321.11	321.0
5	Cl	H	H	H	Cl	C₁₇H₁₈Cl₂N₂O	337.08	337.0
6	F	F	H	H	F	C₁₇H₁₇F₃N₂O	323.13	323.0
7	F	H	F	H	F	C₁₇H₁₇F₃N₂O	323.13	323.0
8	F	H	Cl	H	F	C₁₇H₁₇ClF₂N₂O	339.10	339.0
9	F	H	H	F	Cl	C₁₇H₁₇ClF₂N₂O	339.10	339.0

Example 7

Following the procedures described in the Schemes and/or the examples above, and substituting the appropriate starting materials and reagents, compounds 7-1 to 7-47, having formula Ib″, can also be prepared:


	(Ib″)

Example 8

Following the procedures described in the Schemes and/or the examples above (for example, using the Ullmann reaction), and substituting the appropriate starting materials and reagents, compounds 8-1 to 8-15, having formula Ib′″, can also be prepared:


		(Ib′′′)

Ex.	R²	R³	R⁴	R⁵	R⁶

1	F	H	H	H	F
2	H	H	H	H	H
3	F	H	H	H	H
1	F	H	H	H	F
2	H	H	H	H	H
3	F	H	H	H	H
4	H	Cl	H	H	H
5	F	H	H	H	Cl
6	Cl	H	H	H	Cl
7	F	F	H	H	F
8	F	H	F	H	F
9	F	H	Cl	H	F
10	F	H	H	F	Cl
11	F	H	H	H	Br
12	F	Cl	H	H	F
13	F	H	Br	H	Cl
14	F	H	F	H	Cl
15	F	Me	H	H	F

Preparation 4

4-Hydroxymethyl-3′,4′,5′,6′-tetrahydro-2′H-[3,4′]-bipyridinyl-1′-carboxylic Acid t-Butyl Ester

H₂SO₄(2.5 mL, 48.3 mmol, 1.0 eq.) was added to a solution of 3-bromoisonicotinic acid (9.7 g, 48.3 mmol, 1.0 eq.) in MeOH (150 mL). The resulting solution was stirred for 14 hours while the temperature was maintained at reflux in an oil bath. The mixture was then cooled to room temperature and concentrated under vacuum. The residue was dissolved in EtOAc (200 mL). The pH of the solution was adjusted to 10 with Na₂CO₃. The resulting solution was extracted with EtOAc (100 mL). The organic layer was washed with water (1×200 mL) and saturated aqueous NaCl (2×100 mL). The mixture was dried over anhydrous Na₂SO₄and concentrated under vacuum. The residue was applied onto a silica gel column and eluted with EtOAc/PE (1:20) to yield 3-bromoisonicotinic acid methyl ester (4.4 g) as a colorless liquid.
PdCl₂(PPh₃)₂(0.7 g, 1.03 mmol, 0.05 eq.) was added to a solution of 4-(4,4,5,5-tetramethyl-[1,3,2]dioxaborolan-2-yl)-3,6-dihydro-2H-pyridine-1-carboxylic acid t-butyl ester (6.3 g, 20.5 mmol, 1.0 eq.) in THF (120 mL) under nitrogen. To this was added 3-bromoisonicotinic acid methyl ester (4.4 g, 20.5 mmol, 1.0 eq.). This was followed by the addition of a solution of Na₂CO₃(8.7 g, 82.1 mmol, 4.0 eq.) in water (41 mL). The resulting solution was stirred for 14 hours while the temperature was maintained at reflux in an oil bath. The resulting solution was diluted with EtOAc (100 mL), and washed with saturated aqueous NaCl (2×100 mL). The organic layer was dried over anhydrous Na₂SO₄and concentrated under vacuum. The residue was applied onto a silica gel column and eluted with EtOAc/PE (1:10) to yield 3′,6′-dihydro-2′H-[3,4′]bipyridinyl-4,1′-dicarboxylic acid 1′-t-butyl ester 4-methyl ester (5.6 g) as a yellow oil.
H-NMR: (300 MHz, CDCl₃, ppm): 8.65 (1H, d, J=4.8 Hz), 8.54 (1H, s), 7.65 (1H, d, J=4.8 Hz), 5.65 (1H, m), 4.08 (2H, m), 3.90 (3H, s), 3.65 (2H, m), 2.34 (2H, m), 1.51 (9H, s).
Pd(OH)₂/C (0.4 g) was added to a solution of 3′,6′-dihydro-2′H-[3,4′]bipyridinyl-4,1′-dicarboxylic acid 1′-t-butyl ester 4-methyl ester (500 mg, 1.2 mmol, 1.0 eq.) in EtOAc (15 mL), maintained under hydrogen at 1 atm. The resulting solution was stirred over night at room temperature. The solids were filtered. The resulting mixture was concentrated under vacuum. The residue was applied onto a silica gel column and eluted with EtOAc/PE (1:3) to yield 3′,4′,5′,6′-tetrahydro-2′H-[3,4′]bipyridinyl-4,1′-dicarboxylic acid 1′-t-butyl ester 4-methyl ester (240 mg) as a colorless liquid.
H-NMR: (300 MHz, CDCl₃, ppm): 8.70 (1H, s), 8.59 (1H, d, J=4.8 Hz), 7.61 (1H, d, J=4.8 Hz), 4.27 (2H, m), 3.96 (3H, s), 3.47 (1H, m), 2.82 (2H, m), 1.88 (2H, m), 1.73 (2H, m), 1.51 (9H, s).
A solution of 3′,4′,5′,6′-tetrahydro-2′H-[3,4′]bipyridinyl-4,1′-dicarboxylic acid 1′-t-butyl ester 4-methyl ester (3.3 g, 8.2 mmol, 1.0 eq.) in THF (50 mL) was added dropwise to a solution of LiAlH₄(590 mg, 15.5 mmol, 1.5 eq.) in THF (50 mL) at 0° C. under nitrogen. The resulting solution was stirred for 10 minutes at 0° C. The reaction was then quenched with water (0.6 mL) and 15% NaOH (1.8 mL). The solids were filtered. The mixture was dried over anhydrous Na₂SO₄and concentrated under vacuum. The residue was applied onto a silica gel column and eluted with EtOAc/PE (1:1) to yield the title compound (1.8 g) as a white solid. MS m/z: [M+H]⁺ calcd for C₁₆H₂₅N₂O₃, 293. found 293.
H-NMR: (300 MHz, CDCl₃, ppm): 8.49 (2H, s), 7.42 (1H, d, J=4.8 Hz), 4.82 (2H, s), 4.29 (2H, m), 2.88 (2H, m), 2.82 (2H, t), 1.78 (2H, m), 1.72 (2H, m), 1.51 (9H, s).

Example 9

4-(2,4,6-Trifluorophenoxymethyl)-1′,2′,3′,4′,5′,6′-hexahydro[3,4′]bipyridinyl

PPh₃(28.9 mg, 0.1 mmol, 1.1 eq.) and 4-hydroxymethyl-3′,4′,5′,6′-tetrahydro-2′H-[3,4′]bipyridinyl-1′-carboxylic acid t-butyl ester (29.2 mg, 0.1 mmol, 1.0 eq.) were dissolved in toluene (533 μL). 2,4,6-Trifluorophenol (14.8 mg, 0.1 mmol, 1.0 eq.) was added, followed by the addition of DIAD (23.6 μL, 0.1 mmol, 1.2 eq.) in toluene (5 mL). The mixture was heated at 80° C. for 4 hours, then concentrated using an evaporator. The material was then dissolved in 1.25M HCl in EtOH (1.0 mL), and stirred at room temperature overnight. The material was again concentrated using an evaporator, then dissolved in 1:1 AcOH:H₂O, filtered, and purified by preparative HPLC to yield the title compound as a TFA salt (30.4 mg, 100% purity). MS m/z: [M+H]⁺ calcd for C₁₇H₁₂F₃N₂O, 323.13. found 323.0.

Example 10

Following the procedures described in the Schemes and/or the examples above, and substituting the appropriate starting materials and reagents, compounds 10-1 to 10-9, having formula Ic′, were also prepared:


	(Ic′)

MS m/z: [M + H]⁺

Ex.	R²	R³	R⁴	R⁵	R⁶	Formula	calcd	found

1	H	H	H	H	H	C₁₇H₂₀N₂O	269.16	269.2
2	F	H	H	H	H	C₁₇H₁₉FN₂O	287.15	287.2
3	F	H	H	H	F	C₁₇H₁₈F₂N₂O	305.14	305.2
4	F	H	H	H	Cl	C₁₇H₁₈ClFN₂O	321.11	321.0
5	Cl	H	H	H	Cl	C₁₇H₁₈Cl₂N₂O	337.08	337.0
6	H	Cl	H	H	H	C₁₇H₁₉ClN₂O	303.12	303.0
7	F	F	H	H	F	C₁₇H₁₇F₃N₂O	323.13	323.2
8	F	H	Cl	H	F	C₁₇H₁₇ClF₂N₂O	339.10	339.0
9	F	H	H	F	Cl	C₁₇H₁₇ClF₂N₂O	339.10	339.0

Example 11

Following the procedures described in the Schemes and/or the examples above, and substituting the appropriate starting materials and reagents, compounds 11-1 to 11-47, having formula Ic″, can also be prepared:


	(Ic″)

Example 12

Following the procedures described in the Schemes and/or the examples above (for example, using the Ullmann reaction), and substituting the appropriate starting materials and reagents, compounds 12-1 to 12-15, having formula Ic′″, can also be prepared:


		(Ic′′′)

Ex.	R²	R³	R⁴	R⁵	R⁶

1	F	H	F	H	F
2	H	H	H	H	H
3	F	H	H	H	H
4	F	H	H	H	F
5	F	H	H	H	Cl
6	Cl	H	H	H	Cl
7	H	Cl	H	H	H
8	F	F	H	H	F
9	F	H	Cl	H	F
10	F	H	H	F	Cl
11	F	H	H	H	Br
12	F	Cl	H	H	F
13	F	H	Br	H	Cl
14	F	H	F	H	Cl
15	F	Me	H	H	F

Preparation 5

3-Hydroxymethyl-3′,4′,5′,6′-tetrahydro-2′H-[2,4′]-bipyridinyl-1′-carboxylic Acid t-Butyl Ester

H₂SO₄(0.6 mL) was added to a solution of 2-bromonicotinic acid (1.5 g, 7.43 mmol, 1.00 eq.) in MeOH (20 mL). The resulting solution was stirred for 5 hours while the temperature was maintained at reflux in an oil bath. The mixture was cooled to room temperature and concentrated under vacuum. The resulting solution was diluted with EtOAc (50 mL) and the pH of the solution was adjusted to 10 with Na₂CO₃(20%). The resulting organic layer was washed with water (1×50 mL) and saturated aqueous NaCl (2×100 mL), dried over anhydrous Na₂SO₄, and concentrated under vacuum to yield 2-bromonicotinic acid methyl ester (1.1 g) as a yellow liquid.
2-bromonicotinic acid methyl ester (7.1 g, 33.0 mmol, 1.0 eq.) was combined with a solution of 4-(4,4,5,5-tetramethyl-[1,3,2]dioxaborolan-2-yl)-3,6-dihydro-2H-pyridine-1-carboxylic acid t-butyl ester (10.2 g, 33.0 mmol, 1.0 eq.) in THF (330 mL), under nitrogen. A solution of Na₂CO₃(14 g) in water (66 mL) was then added, followed by the addition of PdCl₂(PPh₃)₂(1.2 g, 1.7 mmol, 0.05 eq.). The resulting solution was stirred for 32 hours while the temperature was maintained at reflux in an oil bath. The solution was then diluted with EtOAc (200 mL) and washed with saturated aqueous NaCl (2×300 mL). The organic layer was dried over anhydrous Na₂SO₄and concentrated under vacuum. The residue was applied onto a silica gel column and eluted with EtOAc/PE (1:10) to yield 3′,6′-dihydro-2′H-[2,4′]bipyridinyl-3,1′-dicarboxylic acid F-t-butyl ester 3-methyl ester (6.0 g) as a yellow oil.
Pd(OH)₂/C (690 mg) was added to a solution of 3′,6′-dihydro-2′H-[2,4′]bipyridinyl-3,1′-dicarboxylic acid 1′-t-butyl ester 3-methyl ester (2.0 g, 6.3 mmol, 1.0 eq.) in PE (220 mL) and EtOAc (50 mL). Hydrogen gas (1 atm) was introduced into the reaction vessel, and the solution was stirred for 20 hours at room temperature. The reaction mixture was filtered and the remaining mixture was concentrated under vacuum. The residue was applied onto a silica gel column and eluted with EtOAc/PE (1:8) to yield 3′,4′,5′,6′-tetrahydro-2′H-[2,4′]bipyridinyl-3,1′-dicarboxylic acid 1′-t-butyl ester 3-methyl ester (1.2 g) as a colorless oil.
NaBH₄(0.6 g, 15.8 mmol, 5 eq.) was added to a solution of 3′,4′,5′,6′-tetrahydro-2′H-[2,4′]bipyridinyl-3,1′-dicarboxylic acid 1′-t-butyl ester 3-methyl ester (1.0 g, 3.1 mmol, 1.0 eq.) in THF (40 mL), under nitrogen. This mixture was cooled to 0-5° C. and MeOH (8 mL) was added dropwise. The resulting solution was stirred for 2.5 hours at room temperature. The solution was diluted with EtOAc (50 mL), then washed with saturated NH₄Cl (1×100 mL) and saturated aqueous NaCl (2×100 mL). The organic layer was dried over anhydrous Na₂SO₄and concentrated under vacuum. The residue was applied onto a silica gel column and eluted with EtOAc/PE (1:5) to yield 3-hydroxymethyl-3′,4′,5′,6′-tetrahydro-2′H-[2,4′]-bipyridinyl-1′-carboxylic acid t-butyl ester (0.4 g) as a white solid. MS m/z: [M+H]⁺ calcd for C₁₆H₂₅N₂O₃, 293. found 293.
H-NMR: (300 MHz, CDCl₃, ppm): 8.52 (1H, m), 7.26 (1H, d, J=7.5), 7.17 (1H, m), 4.80 (2H, s), 4.28 (2H, s), 3.11 (1H, m), 3.03 (2H, m), 2.06 (3H, m), 1.74 (2H, m), 1.48 (9H, s).

Example 13

3-(2,4,6-Trifluorophenoxymethyl)-1′,2′,3′,4′,5′,6′-hexahydro[2,4′]bipyridinyl

PPh₃(28.9 mg, 0.1 mmol, 1.1 eq.) and 3-hydroxymethyl-3′,4′,5′,6′-tetrahydro-2′H-[2,4′]bipyridinyl-1′-carboxylic acid t-butyl ester (29.2 mg, 0.1 mmol, 1.0 eq.) were dissolved in toluene (533 μL). 2,4,6-Trifluorophenol (14.8 mg, 0.1 mmol, 1.0 eq.) was added, followed by the addition of DIAD (23.6 μL, 0.1 mmol, 1.2 eq.) in toluene (5 mL). The mixture was heated at 80° C. for 4 hours, then concentrated using an evaporator. The material was then dissolved in 1.25M HCl in EtOH (1.0 mL), and stirred at room temperature overnight. The material was again concentrated using an evaporator, then dissolved in 1:1 AcOH:H₂O, filtered, and purified by preparative HPLC to yield the title compound as a TFA salt (12.8 mg, 97% purity). MS m/z: [M+H]⁺ calcd for C₁₇H₁₇F₃N₂O, 323.13. found 323.2.

Example 14

Following the procedures described in the Schemes and/or the examples above, and substituting the appropriate starting materials and reagents, compounds 14-1 to 14-9, having formula Id′, were also prepared:


	(Id′)

MS m/z: [M + H]⁺

Example 15

Following the procedures described in the Schemes and/or the examples above, and substituting the appropriate starting materials and reagents, compounds 15-1 to 15-48, having formula Id″, can also be prepared:


	(Id″)

Ex.	R^1a	R^1b	R^1c	R²	R³	R⁴	R⁵	R⁶

1	H	H	H	F	H	H	H	Br
2	H	H	H	F	H	F	H	F
3	H	H	H	F	Cl	H	H	F
4	H	H	H	F	H	Br	H	Cl
5	H	H	H	F	H	F	H	Cl
6	H	H	H	F	Me	H	H	F
7	F	H	H	F	H	H	H	H
8	H	F	H	F	H	H	H	H
9	H	H	F	F	H	H	H	H
10	F	H	H	F	H	H	H	F
11	H	F	H	F	H	H	H	F
12	H	H	F	F	H	H	H	F
13	F	H	H	F	H	H	H	Cl
14	H	F	H	F	H	H	H	Cl
15	H	H	F	F	H	H	H	Cl
16	F	H	H	F	F	H	H	F
17	H	F	H	F	F	H	H	F
18	H	H	F	F	F	H	H	F
19	F	H	H	F	H	F	H	F
20	H	F	H	F	H	F	H	F
21	H	H	F	F	H	F	H	F
22	F	H	H	F	H	H	F	Cl
23	H	F	H	F	H	H	F	Cl
24	H	H	F	F	H	H	F	Cl
25	—CF₃	H	H	F	H	H	H	Cl
26	H	—CF₃	H	F	H	H	H	Cl
27	H	H	—CF₃	F	H	H	H	Cl
28	—CF₃	H	H	F	F	H	H	F
29	H	—CF₃	H	F	F	H	H	F
30	H	H	—CF₃	F	F	H	H	F
31	—CF₃	H	H	F	H	F	H	F
32	H	—CF₃	H	F	H	F	H	F
33	H	H	—CF₃	F	H	F	H	F
34	F	F	H	F	H	H	H	F
35	F	H	F	F	H	H	H	F
36	H	F	F	F	H	H	H	F
37	F	F	H	F	H	H	H	Cl
38	F	H	F	F	H	H	H	Cl
39	H	F	F	F	H	H	H	Cl
40	F	F	H	F	H	F	H	F
41	F	H	F	F	H	F	H	F
42	H	F	F	F	H	F	H	F
43	F	F	H	F	F	H	H	F
44	F	H	F	F	F	H	H	F
45	H	F	F	F	F	H	H	F
46	F	F	H	F	H	H	F	Cl
47	F	H	F	F	H	H	F	Cl
48	H	F	F	F	H	H	F	Cl

Example 16

Following the procedures described in the Schemes and/or the examples above, and substituting the appropriate starting materials and reagents, compounds 16-1 to 16-16, having formula Id′″, can also be prepared:


	(Id′′′)

Ex.	R²	R³	R⁴	R⁵	R⁶

1	F	H	F	H	F
2	H	H	H	H	H
3	F	H	H	H	H
4	H	Cl	H	H	H
5	F	H	H	H	F
6	F	H	H	H	Cl
7	Cl	H	H	H	Cl
8	F	F	H	H	F
9	F	H	Cl	H	F
10	F	H	H	F	Cl
11	F	H	H	H	Br
12	F	H	F	H	F
13	F	Cl	H	H	F
14	F	H	Br	H	Cl
15	F	H	F	H	Cl
16	F	Me	H	H	F

Preparation 6

4-[2-(Toluene-4-sulfonyloxymethyl)furan-3-yl]piperidine-1-carboxylic Acid t-Butyl Ester

3-Bromofuran-2-carboxylic acid methyl ester (2.0 g, 9.8 mmol, 1.0 eq.), 4-(4,4,5,5-tetramethyl-[1,3,2]dioxaborolan-2-yl)-3,6-dihydro-2H-pyridine-1-carboxylic acid t-butyl ester (3.1 g, 10 mmol, 1.0 eq.), THF (100 mL) and Na₂CO₃(20 mL, 40 mmol, 4.0 eq.) were combined. The mixture was degassed and purged with nitrogen (3×). PdCl₂(PPh₃)₂(0.2 g, 0.3 mmol, 0.03 eq.) was added, and the mixture was again degassed and purged with nitrogen (3×), then heated at 80° C. overnight. The mixture was then cooled and the layers were separated. The THF layer was diluted with EtOAc (100 mL), washed with saturated aqueous NaCl (50 mL) and dried over Na₂SO₄. The material was filtered and applied onto a silica gel column and purified by chromatography with PE/EtOAc (50:1 to 10:1) to yield 4-(2-methoxycarbonylfuran-3-yl)-3,6-dihydro-2H-pyridine-1-carboxylic acid t-butyl ester (2.1 g) as a white solid.
A solution of 4-(2-methoxycarbonylfuran-3-yl)-3,6-dihydro-2H-pyridine-1-carboxylic acid t-butyl ester (2.1 g, 6.8 mmol, 1.0 eq.) and Raney Ni (1.0 g) in MeOH (150 mL) was degassed and purged with hydrogen (3×). The mixture was hydrogenated (1 atm) for 2 days, then purged with nitrogen, filtered and washed with MeOH (50 mL). The filtrate was concentrated to yield 4-(2-methoxycarbonylfuran-3-yl)piperidine-1-carboxylic acid t-butyl ester (2.0 g) as a colorless oil.
A solution of 4-(2-methoxycarbonylfuran-3-yl)piperidine-1-carboxylic acid t-butyl ester (2.0 g, 6.5 mmol, 1.0 eq.) in THF (60 mL) was added to a solution of LiAlH₄(490 mg, 13 mmol, 2.0 eq.) in THF (100 mL) at −30° C. The resulting solution was stirred for 1 hour and allowed to warm to 0° C., then stirred for 1 hour at 0° C. Water (0.5 mL) was added dropwise to quench the reaction. The mixture was allowed to warm to room temperature, and 15% NaOH (0.5 mL) and water (1.5 mL) was added. The solids were filtered and the filter cake was washed with EtOAc (50 mL). The filtrate was concentrated and the residue was applied onto a silica gel column and purified by chromatography with PE/EtOAc (10:1˜5:1), then by preparative HPLC to yield 4-(2-hydroxymethylfuran-3-yl)piperidine-1-carboxylic acid t-butyl ester (1.1 g) as a yellow oil.
4-(2-Hydroxymethylfuran-3-yl)piperidine-1-carboxylic acid t-butyl ester (0.3 g, 1.1 mmol, 1.0 eq.) was dissolved in DCM (5.7 mL). The mixture was cooled to 0° C. and DABCO (132 mg, 1.2 mmol, 1.1 eq.) was added, followed by TsCl (224 mg, 1.2 mmol, 1.1 eq.). The mixture was stirred at 0° C. for 90 minutes, then diluted with EtOAc (500 ml) and washed with water (2×250 mL). The organic layer was dried over anhydrous Na₂SO₄, filtered and concentrated by rotary evaporation to yield the title compound (340 mg), which was used without further purification.

Example 17

4-(2-Phenoxymethylfuran-3-yl)piperidine

4-[2-(Toluene-4-sulfonyloxymethyl)furan-3-yl]piperidine-1-carboxylic acid t-butyl ester (65 mg, 0.15 mmol, 1.0 eq.) was dissolved in MeCN (770 μL) and added to a mixture of potassium carbonate (32 mg, 230 mmol, 1.6 eq.) and phenol (21.1 mg, 224 μmol, 1.5 eq.). The resulting mixture was shaken at 50° C. overnight. The mixture was then cooled and the solids removed. The supernatant was combined with TFA (12 μL, 160 mmol, 1.0 eq.) and the resulting mixture was shaken overnight at room temperature. The solution was concentrated using an evaporator, then dissolved in 1:1 AcOH:H₂O (5.0 mL) then an additional 2.0 ml AcOH, filtered, and purified by preparative HPLC to yield the title compound as a TFA salt (1.4 mg, 88% purity). MS m/z: [M+H]⁺ calcd for C₁₆H₁₉NO₂, 258.14. found 258.2.

Example 18

Following the procedures described in the Schemes and/or the examples above, and substituting the appropriate starting materials and reagents, compounds 18-1 to 18-3, having formula Ie′, were also prepared:


	(Ie′)

MS m/z: [M + H]⁺

Ex.	R²	R³	R⁴	R⁵	R⁶	Formula	calcd	found

1	Cl	H	H	H	Cl	C₁₆H₁₇Cl₂NO₂	326.06	326.0
2	H	Cl	H	H	H	C₁₆H₁₈ClNO₂	292.10	292.0
3	F	H	H	H	F	C₁₆H₁₇F₂NO₂	294.12	294.2

Example 19

Following the procedures described in the Schemes and/or the examples above, and substituting the appropriate starting materials and reagents, compounds 19-1 to 19-34, having formula Ie″, can also be prepared:


	(Ie″)

Ex.	R^1a	R^1b	R²	R³	R⁴	R⁵	R⁶

1	H	H	F	H	H	H	H
2	H	H	F	H	H	H	Cl
3	H	H	F	H	H	H	Br
4	H	H	F	H	F	H	F
5	H	H	F	F	H	H	F
6	H	H	F	H	Cl	H	F
7	H	H	F	H	H	F	Cl
8	H	H	F	Cl	H	H	F
9	H	H	F	H	Br	H	Cl
10	H	H	F	H	F	H	Cl
11	H	H	F	Me	H	H	F
12	F	H	F	H	H	H	H
13	H	F	F	H	H	H	H
14	F	H	F	H	H	H	F
15	H	F	F	H	H	H	F
16	F	H	F	H	H	H	Cl
17	H	F	F	H	H	H	Cl
18	F	H	F	F	H	H	F
19	H	F	F	F	H	H	F
20	F	H	F	H	F	H	F
21	H	F	F	H	F	H	F
22	F	H	F	H	H	F	Cl
23	H	F	F	H	H	F	Cl
24	—CF₃	H	F	H	H	H	Cl
25	H	—CF₃	F	H	H	H	Cl
26	—CF₃	H	F	F	H	H	F
27	H	—CF₃	F	F	H	H	F
28	—CF₃	H	F	H	F	H	F
29	H	—CF₃	F	H	F	H	F
30	F	F	F	H	H	H	F
31	F	F	F	H	H	H	Cl
32	F	F	F	H	F	H	F
33	F	F	F	F	H	H	F
34	F	F	F	H	H	F	Cl

Example 20

Following the procedures described in the Schemes and/or the examples above, and substituting the appropriate starting materials and reagents, compounds 20-1 to 20-35, having formula If″, can also be prepared:


	(If″)

Ex.	R^1a	R^1b	R²	R³	R⁴	R⁵	R⁶

1	H	H	F	H	F	H	F
2	H	H	F	H	H	H	F
3	H	H	F	H	H	H	H
4	H	H	F	H	Cl	H	F
5	H	H	F	F	H	H	F
6	H	H	F	Cl	H	H	F
7	H	H	F	—CH₃	H	H	F
8	H	H	F	H	H	F	Cl
9	H	H	F	H	H	H	Cl
10	H	H	F	H	H	H	Br
11	H	H	F	H	Br	H	Cl
12	H	H	F	H	F	H	Cl
13	F	H	F	H	H	H	H
14	H	F	F	H	H	H	H
15	F	H	F	H	H	H	F
16	H	F	F	H	H	H	F
17	F	H	F	H	H	H	Cl
18	H	F	F	H	H	H	Cl
19	F	H	F	F	H	H	F
20	H	F	F	F	H	H	F
21	F	H	F	H	F	H	F
22	H	F	F	H	F	H	F
23	F	H	F	H	H	F	Cl
24	H	F	F	H	H	F	Cl
25	—CF₃	H	F	H	H	H	Cl
26	H	—CF₃	F	H	H	H	Cl
27	—CF₃	H	F	F	H	H	F
28	H	—CF₃	F	F	H	H	F
29	—CF₃	H	F	H	F	H	F
30	H	—CF₃	F	H	F	H	F
31	F	F	F	H	H	H	F
32	F	F	F	H	H	H	Cl
33	F	F	F	H	F	H	F
34	F	F	F	F	H	H	F
35	F	F	F	H	H	F	Cl

Preparation 7

4-[3-(Toluene-4-sulfonyloxymethyl)furan-2-yl]-piperidine-1-carboxylic Acid t-Butyl Ester
2-Bromofuran-3-carboxylic acid (5.0 g, 26 mmol, 1 eq.) was dissolved in MeOH (100 mL) and the mixture was degassed and purged with nitrogen (2×). SO₂Cl₂(15 mL, 0.2 mol, 7.7 eq.) was added dropwise and the resulting mixture was stirred for two days at room temperature. The mixture was then concentrated and DCM (150 mL) was added. Saturated NaHCO₃(20 mL) was added, and the resulting mixture was stirred at room temperature for 15 minutes. The organic layer was washed with saturated NaHCO₃(20 mL), water (20 mL), and saturated aqueous NaCl (20 mL), then dried over Na₂SO₄, filtered and concentrated to yield 2-bromofuran-3-carboxylic acid methyl ester (5.0 g) as a brown solid.
2-Bromofuran-3-carboxylic acid methyl ester (3.0 g, 15 mmol, 1.0 eq.), 4-(4,4,5,5-tetramethyl-[1,3,2]dioxaborolan-2-yl)-3,6-dihydro-2H-pyridine-1-carboxylic acid t-butyl ester (4.5 g, 15 mmol, 1.0 eq.), THF (150 mL) and Na₂CO₃(30 mL, 60 mmol, 4.0 eq.) were combined. The mixture was degassed and purged with nitrogen (3×). PdCl₂(PPh₃)₂(0.3 g, 0.4 mmol, 0.03 eq.) was added, and the mixture was again degassed and purged with nitrogen (3×), then heated at 80° C. overnight. The mixture was then cooled and the layers were separated. The THF layer was diluted with EtOAc (150 mL), washed with saturated aqueous NaCl (80 mL) and dried over Na₂SO₄. The material was filtered and applied onto a silica gel column and purified by chromatography with PE/EtOAc (20:1 to 10:1) to yield 4-(3-methoxycarbonylfuran-2-yl)-3,6-dihydro-2H-pyridine-1-carboxylic acid t-butyl ester (3.5 g) as a white solid.
A solution of 4-(3-methoxycarbonylfuran-2-yl)-3,6-dihydro-2H-pyridine-1-carboxylic acid t-butyl ester (0.8 g, 2.1 mmol, 1.0 eq.) and Raney Ni (0.4 g) in MeOH (50 mL) was degassed and purged with hydrogen (3×). The mixture was hydrogenated (1 atm) for 1 hour, then purged with nitrogen, filtered and washed with MeOH (15 mL). The filtrate was concentrated to yield 4-(3-methoxycarbonylfuran-2-yl)piperidine-1-carboxylic acid t-butyl ester (550 mg) as a white solid.
A solution of 4-(3-methoxycarbonylfuran-2-yl)piperidine-1-carboxylic acid t-butyl ester (550 mg, 1.6 mmol, 1.0 eq.) in THF (15 mL) was added to a solution of LiAlH₄(120 mg, 3.2 mmol, 2.0 eq.) in THF (25 mL) at −30° C. The resulting solution was stirred for 1 hour and allowed to warm to 0° C., then stirred for 1 hour at 0° C. Water (120 mg) was added dropwise to quench the reaction. The mixture was allowed to warn to room temperature, and 15% NaOH (360 mg) and water (120 mg) was added. The mixture was stirred for 1 hour at room temperature. The solids were filtered and the filter cake was washed with EtOAc (20 mL). The residue was applied onto a silica gel column and purified by chromatography with PE/EtOAc (10:1˜5:1). The product was combined with another lot of material, then crystallized (PE/EtOAc=3:1, 1.2 mL), to yield 4-(3-hydroxymethylfuran-2-yl)piperidine-1-carboxylic acid t-butyl ester (0.8 g) as a white solid.
4-(3-Hydroxymethylfuran-2-yl)piperidine-1-carboxylic acid t-butyl ester (0.3 g, 1.1 mmol, 1.0 eq.) was dissolved in DCM (5.7 mL). The mixture was cooled to 0° C. and DABCO (132 mg, 1.2 mmol, 1.1 eq.) was added, followed by TsCl (224 mg, 1.2 mmol, 1.1 eq.). The mixture was stirred at 0° C. for 90 minutes, then diluted with EtOAc (500 mL) and washed with water (2×250 mL). The organic layer was dried over anhydrous Na₂SO₄, filtered and concentrated by rotary evaporation to yield the title compound (233 mg), which was used without further purification.

Example 21

4-[3-(3-Chlorophenoxymethyl)furan-2-yl]piperidine

4-[2-(Toluene-4-sulfonyloxymethyl)furan-3-yl]piperidine-1-carboxylic acid t-butyl ester (65 mg, 0.15 mmol was dissolved in MeCN (770 μL) and added to a mixture of potassium carbonate (32 mg, 230 mmol, 1.6 eq.) and 3-chlorophenol (28.8 mg, 224 μmol, 1.5 eq.). The resulting mixture was shaken at 50° C. overnight. The mixture was then cooled and the solids removed. The supernatant was combined with TFA (12 μL, 160 μmol, 1.0 eq.) and the resulting mixture was shaken overnight at room temperature. The solution was concentrated using an evaporator, then dissolved in 1:1 AcOH:H₂O (5.0 mL) then an additional 2.0 ml AcOH, filtered, and purified by preparative HPLC to yield the title compound as a TFA salt (4.3 mg, 98% purity). MS m/z: [M+H]⁺ calcd for C₁₆H₁₈ClNO₂, 292.10. found 292.0.

Example 22

Following the procedures described in the Schemes and/or the examples above, and substituting the appropriate starting materials and reagents, compounds 22-1 to 22-4, having formula Ig′, were also prepared:


	(Ig′)

MS m/z: [M + H]⁺

Ex.	R²	R³	R⁴	R⁵	R⁶	Formula	calcd	found

1	H	H	H	H	H	C₁₆H₁₉NO₂	258.14	258.2
2	F	H	H	H	F	C₁₆H₁₇F₂NO₂	294.12	294.0
3	Cl	H	H	H	Cl	C₁₆H₁₇Cl₂NO₂	326.06	326.0
4	F	H	F	H	F	C₁₆H₁₆F₃NO₂	312.11	312.0

Example 23

Following the procedures described in the Schemes and/or the examples above, and substituting the appropriate starting materials and reagents, compounds 23-1 to 23-33, having formula Ig″, can also be prepared:


	(Ig″)

Ex.	R^1a	R^1b	R²	R³	R⁴	R⁵	R⁶

1	H	H	F	H	H	H	H
2	H	H	F	H	H	H	Cl
3	H	H	F	H	H	H	Br
4	H	H	F	F	H	H	F
5	H	H	F	H	Cl	H	F
6	H	H	F	H	H	F	Cl
7	H	H	F	Cl	H	H	F
8	H	H	F	H	Br	H	Cl
9	H	H	F	H	F	H	Cl
10	H	H	F	Me	H	H	F
11	F	H	F	H	H	H	H
12	H	F	F	H	H	H	H
13	F	H	F	H	H	H	F
14	H	F	F	H	H	H	F
15	F	H	F	H	H	H	Cl
16	H	F	F	H	H	H	Cl
17	F	H	F	F	H	H	F
18	H	F	F	F	H	H	F
19	F	H	F	H	F	H	F
20	H	F	F	H	F	H	F
21	F	H	F	H	H	F	Cl
22	H	F	F	H	H	F	Cl
23	—CF₃	H	F	H	H	H	Cl
24	H	—CF₃	F	H	H	H	Cl
25	—CF₃	H	F	F	H	H	F
26	H	—CF₃	F	F	H	H	F
27	—CF₃	H	F	H	F	H	F
28	H	—CF₃	F	H	F	H	F
29	F	F	F	H	H	H	F
30	F	F	F	H	H	H	Cl
31	F	F	F	H	F	H	F
32	F	F	F	F	H	H	F
33	F	F	F	H	H	F	Cl

Preparation 8

4-(2-Hydroxymethylthiophen-3-yl)piperidine-1-carboxylic Acid t-Butyl Ester

3-Bromothiophene-2-carboxylic acid (5.0 g, 24 mmol, 1 eq.) was dissolved in MeOH (100 mL) and the mixture was degassed and purged with nitrogen (2×). SOCl₂(15 mL, 0.2 mol, 8.3 eq.) was added dropwise and the resulting mixture was stirred overnight at room temperature. The mixture was then concentrated and DCM (150 mL) was added. Saturated NaHCO₃(20 mL) was added, and the resulting mixture was stirred at room temperature for 15 minutes. The organic layer was washed with saturated NaHCO₃(20 mL), water (20 mL), and saturated aqueous NaCl (20 mL), then dried over Na₂SO₄, filtered and concentrated to yield 3-bromothiophene-2-carboxylic acid methyl ester (4.0 g) as a yellow solid.
3-Bromothiophene-2-carboxylic acid methyl ester (1.0 g, 4.5 mmol, 1.0 eq.), 4-(4,4,5,5-tetramethyl-[1,3,2]dioxaborolan-2-yl)-3,6-dihydro-2H-pyridine-1-carboxylic acid t-butyl ester (1.4 g, 4.5 mmol, 1.0 eq.), THF (50 mL) and Na₂CO₃(9 mL, 18 mmol, 4.0 eq.) were combined. The mixture was degassed and purged with nitrogen (3×). PdCl₂(PPh₃)₂(10.1 g, 0.14 mmol, 0.03 eq.) was added, and the mixture was again degassed and purged with nitrogen (3×), then heated at 80° C. overnight. The mixture was then cooled and the layers were separated. The THF layer was diluted with EtOAc (50 mL), washed with saturated aqueous NaCl (20 mL) and dried over Na₂SO₄. The material was filtered and applied onto a silica gel column and purified by chromatography with PE/EtOAc (20:1˜10:1) to yield 4-(2-methoxycarbonylthiophen-3-yl)-3,6-dihydro-2H-pyridine-1-carboxylic acid t-butyl ester (0.5 g) as an oil.
A solution of 4-(2-methoxycarbonylthiophen-3-yl)-3,6-dihydro-2H-pyridine-1-carboxylic acid t-butyl ester (2.5 g, 7.7 mmol, 1.0 eq.) and Pd(OH)₂/C (0.5 g, 0.6 mmol) in MeOH (70 mL) was degassed and purged with hydrogen (3×). The mixture was hydrogenated (4 atm) at room temperature overnight, then the vessel was evacuated and purged with nitrogen, and the material was filtered and washed with MeOH (150 mL). The remaining material was concentrated then again was hydrogenated (4 atm) at room temperature overnight. Filtering again as above, and concentrating the filtrate provided 4-(2-methoxycarbonylthiophen-3-yl)piperidine-1-carboxylic acid t-butyl ester (2.4 g).
A solution of 4-(2-methoxycarbonylthiophen-3-yl)piperidine-1-carboxylic acid t-butyl ester (2.4 g, 7.4 mmol, 1.0 eq.) in THF (20 mL) was degassed and purged with nitrogen (2×). Borane dimethyl sulfide complex (35 mL, 70 mmol, 110.8 eq.) was added at room temperature and the mixture was heated to reflux overnight. The mixture was allowed to cool to room temperature and MeOH (20 mL) was slowly added. The mixture was then concentrated under vacuum. The addition of MeOH (20 mL) and concentrating was repeated two additional times. The product was applied onto a silica gel column and purified by chromatography with PE/EtOAc (10:1). The product was then purified by preparative HPLC to yield the title compound (1.5 g) as a yellow oil, which gradually solidified.

Example 24

4-[2-(3-Chlorophenoxymethyl)thiophen-3-yl]piperidine

PPh₃(28.9 mg, 0.1 mmol, 1.1 eq.) and 4-(2-hydroxymethylthiophen-3-yl)piperidine-1-carboxylic acid t-butyl ester (29.7 mg, 0.1 mmol; 1.0 eq.) were dissolved in toluene (533 μL). 3-Chlorophenol (12.9 mg, 0.1 mmol, 1.0 eq.) was added, followed by the addition of DIAD (23.6 μL, 0.1 mmol, 1.2 eq.) in toluene (5 mL). The reactions were heated at 80° C. for 4 hours, then concentrated using an evaporator. The material was then dissolved in 1.25M HCl in EtOH (1 mL), and stirred at room temperature overnight. The material was again concentrated using an evaporator, then dissolved in 1:1 AcOH:H₂O, filtered, and purified by preparative HPLC to yield the title compound as a TFA salt (3.2 mg, 100% purity). MS m/z: [M+H]⁺ calcd for C₁₆H₁₈ClNOS, 308.08. found 308.0.

Example 25

Following the procedures described in the Schemes and/or the examples above, and substituting the appropriate starting materials and reagents, compounds 25-1 to 25-5, having formula Ih′, were also prepared:


	(Ih′)

MS m/z: [M + H]⁺

Ex.	R²	R³	R⁴	R⁵	R⁶	Formula	calcd	found

1	Cl	H	H	H	Cl	C₁₆H₁₇Cl₂NOS	342.04	342.0
2	F	H	H	H	F	C₁₆H₁₇F₂NOS	310.10	310.0
3	F	H	H	H	Cl	C₁₆H₁₇ClFNOS	326.07	326.0
4	F	F	H	H	F	C₁₆H₁₆F₃NOS	328.09	328.0
5	F	H	H	F	Cl	C₁₆H₁₆ClF₂NOS	344.06	344.0

Example 26

Following the procedures described in the Schemes and/or the examples above, and substituting the appropriate starting materials and reagents, compounds 26-1 to 26-31, having formula Ih″, can also be prepared:


	(Ih″)

Ex.	R^1a	R^1b	R²	R³	R⁴	R⁵	R⁶

1	H	H	F	H	H	H	H
2	H	H	F	H	H	H	Br
3	H	H	F	H	F	H	F
4	H	H	F	H	Cl	H	F
5	H	H	F	Cl	H	H	F
6	H	H	F	H	Br	H	Cl
7	H	H	F	H	F	H	Cl
8	H	H	F	Me	H	H	F
9	F	H	F	H	H	H	H
10	H	F	F	H	H	H	H
11	F	H	F	H	H	H	F
12	H	F	F	H	H	H	F
13	F	H	F	H	H	H	Cl
14	H	F	F	H	H	H	Cl
15	F	H	F	F	H	H	F
16	H	F	F	F	H	H	F
17	F	H	F	H	F	H	F
18	H	F	F	H	F	H	F
19	F	H	F	H	H	F	Cl
20	H	F	F	H	H	F	Cl
21	—CF₃	H	F	H	H	H	Cl
22	H	—CF₃	F	H	H	H	Cl
23	—CF₃	H	F	F	H	H	F
24	H	—CF₃	F	F	H	H	F
25	—CF₃	H	F	H	F	H	F
26	H	—CF₃	F	H	F	H	F
27	F	F	F	H	H	H	F
28	F	F	F	H	H	H	Cl
29	F	F	F	H	F	H	F
30	F	F	F	F	H	H	F
31	F	F	F	H	H	F	Cl

Preparation 9

4-(4-Hydroxymethylthiophen-3-yl)piperidine-1-carboxylic Acid t-Butyl Ester

A solution of isopropylmagnesium chloride (1.0 g, 9.7 mmol, 1.2 eq.) in THF (5 mL) was added to a solution of 3,4-dibromo-thiophene (2 g, 8.3 mmol, 1.0 eq.) in THF (15 mL), and stirred for 3 hours while the temperature was maintained at 0-5° C. in an ice/salt bath. Methyl chloroformate (1.6 g, 16.9 mmol, 2 eq.) was added dropwise with stirring. The resulting solution was stirred for an additional 16 hours at room temperature. The reaction was then quenched with water (1 mL). The resulting mixture was concentrated under vacuum, then diluted with EtOAc (50 mL). The resulting mixture was washed 1N HCl (1×100 mL) and saturated aqueous NaCl (2×100). The organic layer was concentrated to provide a crude residue, which was applied onto a silica gel column and eluted with EtOAc/PE (1:50) to yield 4-bromothiophene-3-carboxylic acid methyl ester (1.0 g) as a light yellow solid.
H-NMR: (300 MHz, CDCl₃, ppm): 8.12 (1H, d, J=3.6 Hz), 7.32 (1H, d, J=3.6 Hz), 3.88 (3H, s).
A solution of 4-bromothiophene-3-carboxylic acid methyl ester (500 mg, 2.3 mmol, 1.0 eq.) in THF (40 mL) was combined with 4-(4,4,5,5-tetramethyl-[1,3,2]dioxaborolan-2-yl)-3,6-dihydro-2H-pyridine-1-carboxylic acid t-butyl ester (0.7 g, 2.3 mmol, 1 eq.) under nitrogen. A solution of Na₂CO₃(960 mg) in water (4.5 mL) was added, followed by the addition of PdCl₂(PPh₃)₂(80 mg, 0.1 mmol, 0.4 eq.). The resulting solution was stirred for 18 hours while the temperature was maintained at reflux in an oil bath. The resulting solution was diluted with EtOAc (50 mL), then washed with saturated aqueous NaCl (2×100 mL). The organic layer was concentrated and the residue was applied onto a silica gel column and eluted with EtOAc/PE (1:30) to yield 4-(4-methoxycarbonylthiophen-3-yl)-3,6-dihydro-2H-pyridine-1-carboxylic acid t-butyl ester (0.4 g) as a light yellow oil.
H-NMR: (300 MHz, CDCl₃, ppm): 8.10 (1H, d, J=3.6 Hz), 7.05 (1H, d, J=3.6 Hz), 4.15 (2H, m), 3.85 (3H, s), 3.66 (2H, m), 2.38 (2H, s), 1.51 (9H, s).
A solution of 4-(4-methoxycarbonylthiophen-3-yl)-3,6-dihydro-2H-pyridine-1-carboxylic acid t-butyl ester (200 mg, 620 μmol, 1.0 eq.) in EtOAc (25 mL) was combined with Pd(OH)₂/C (70 mg) under hydrogen (1 atm). MeOH (10 mL) was added and the resulting solution was stirred for 20 hours at room temperature. The solution was then stirred for an additional 16 hours while the temperature was maintained at 40° C. in an oil bath. The solids were filtered and the filtrate was concentrated under vacuum. The residue was applied onto a silica gel column and eluted with EtOAc/PE (1:20) to yield 4-(4-methoxycarbonylthiophen-3-yl)-piperidine-1-carboxylic acid t-butyl ester (0.1 g) as a colorless oil.
H-NMR: (400 MHz, CDCl₃, ppm): 8.13 (1H, d, J=3.6 Hz), 7.00 (1H, d, J=2.8 Hz), 4.12 (2H, m), 3.86 (3H, s), 3.50 (1H, m), 2.85 (2H, m), 1.97 (2H, d), 1.51 (12H, s).
A solution of 4-(4-methoxycarbonylthiophen-3-yl)-piperidine-1-carboxylic acid t-butyl ester (100 mg, 310 μmol, 1.0 eq.) in THF (5 mL) was combined with a solution of LiAlH₄(23 mg) under nitrogen. The resulting solution was stirred for 30 minutes while the temperature was maintained at 0-5° C. in an ice/salt bath. The reaction was then quenched by the addition of water (50 μL) and aqueous NaOH (150 μL). The solids were filtered and the filtrate was concentrated under vacuum. The residue was applied onto a silica gel column and eluted with EtOAc/PE (1:10) to yield the titled compound (50 mg) as a colorless oil. MS m/z: [M+H]⁺ calcd for C₁₅H₂₄NO₃S, 298. found 298.
H-NMR: (300 MHz, CDCl₃, ppm): 7.28 (1H, d, J=3.3 Hz), 7.00 (1H, d, J=3 Hz), 4.68 (2H, s), 4.26 (2H, m), 2.82 (3H, m), 1.93 (2H, m), 1.58 (3H, m), 1.50 (9H, s).

Example 27

4-[4-(2,6-Difluorophenoxymethyl)thiophen-3-yl]piperidine

PPh₃(28.9 mg, 0.1 mmol, 1.1 eq.) and 4-(4-hydroxymethylthiophen-3-yl)piperidine-1-carboxylic acid t-butyl ester (29.7 mg, 0.1 mmol; 1.0 eq.) were dissolved in toluene (533 μL). 2,6-Difluorophenol (13.0 mg, 0.1 mmol, 1.0 eq.) was added, followed by the addition of DIAD (23.6 μL, 0.1 mmol, 1.2 eq.) in toluene (5 mL). The mixture was heated at 80° C. for 4 hours, then concentrated using an evaporator. The material was then dissolved in 1.25M HCl in EtOH (1 mL), and stirred at room temperature overnight. The material was again concentrated using an evaporator, then dissolved in 1:1 AcOH:H₂O, filtered, and purified by preparative HPLC to yield the title compound as a TFA salt (4.5 mg, 100% purity). MS m/z: [M+H]⁺ calcd for C₁₆H₁₂F₂NOS, 310.10. found 310.0.

Example 28

Following the procedures described in the Schemes and/or the examples above, and substituting the appropriate starting materials and reagents, compounds 28-1 to 28-5, having formula Ii′, were also prepared:


	(Ii′)

MS m/z: [M + H]⁺

Ex.	R²	R³	R⁴	R⁵	R⁶	Formula	calcd	found

1	F	H	H	H	H	C₁₆H₁₈FNOS	292.11	292.0
2	H	Cl	H	H	H	C₁₆H₁₈ClNOS	308.08	308.0
3	F	H	F	H	F	C₁₆H₁₆F₃NOS	328.09	328.0
4	F	H	Cl	H	F	C₁₆H₁₆ClF₂NOS	344.06	344.0
5	F	H	H	F	Cl	C₁₆H₁₆ClF₂NOS	344.06	344.0

Example 29

Following the procedures described in the Schemes and/or the examples above, and substituting the appropriate starting materials and reagents, compounds 29-1 to 29-30, having formula Ii″, can also be prepared:


	(Ii″)

Ex.	R^1a	R^1b	R²	R³	R⁴	R⁵	R⁶

1	H	H	F	H	H	H	Cl
2	H	H	F	H	H	H	Br
3	H	H	F	F	H	H	F
4	H	H	F	Cl	H	H	F
5	H	H	F	H	Br	H	Cl
6	H	H	F	H	F	H	Cl
7	H	H	F	Me	H	H	F
8	F	H	F	H	H	H	H
9	H	F	F	H	H	H	H
10	F	H	F	H	H	H	F
11	H	F	F	H	H	H	F
12	F	H	F	H	H	H	Cl
13	H	F	F	H	H	H	Cl
14	F	H	F	F	H	H	F
15	H	F	F	F	H	H	F
16	F	H	F	H	F	H	F
17	H	F	F	H	F	H	F
18	F	H	F	H	H	F	Cl
19	H	F	F	H	H	F	Cl
20	—CF₃	H	F	H	H	H	Cl
21	H	—CF₃	F	H	H	H	Cl
22	—CF₃	H	F	F	H	H	F
23	H	—CF₃	F	F	H	H	F
24	—CF₃	H	F	H	F	H	F
25	H	—CF₃	F	H	F	H	F
26	F	F	F	H	H	H	F
27	F	F	F	H	H	H	Cl
28	F	F	F	H	F	H	F
29	F	F	F	F	H	H	F
30	F	F	F	H	H	F	Cl

Preparation 10

4-(3-Hydroxymethylthiophen-2-yl)piperidine-1-carboxylic Acid t-Butyl Ester

2-Bromothiophene-3-carboxylic acid (5.0 g, 24 mmol, 1 eq.) was dissolved in MeOH (100 mL) and the mixture was degassed and purged with nitrogen (2×). SOCl₂(15 mL, 0.2 mol, 8.3 eq.) was added dropwise and the resulting mixture was stirred for two days at room temperature. The mixture was then concentrated and DCM (150 mL) was added. Saturated NaHCO₃(20 mL) was added, and the resulting mixture was stirred at room temperature for 15 minutes. The organic layer was washed with saturated NaHCO₃(20 mL), water (20 mL), and saturated aqueous NaCl (20 mL), then dried over Na₂SO₄, filtered and concentrated to yield 2-bromothiophene-3-carboxylic acid methyl ester (5.1 g) as a yellow oil.
2-Bromothiophene-3-carboxylic acid methyl ester (3.0 g, 14.0 mmol, 1.0 eq.), 4-(4,4,5,5-tetramethyl-[1,3,2]dioxaborolan-2-yl)-3,6-dihydro-2H-pyridine-1-carboxylic acid t-butyl ester (4.2 g, 14.0 mmol, 1.0 eq.), THF (150 mL) and Na₂CO₃(28 mL, 56 mmol, 4.0 eq.) were combined. The mixture was degassed and purged with nitrogen (3×). PdCl₂(PPh₃)₂(0.3 g, 0.4 mmol, 0.03 eq.) was added, and the mixture was again degassed and purged with nitrogen (3×), then heated at 80° C. overnight. The mixture was then cooled and the layers were separated. The THF layer was diluted with EtOAc (150 mL), washed with saturated aqueous NaCl (50 mL) and dried over Na₂SO₄. The material was filtered and applied onto a silica gel column and purified by chromatography with PE/EtOAc (20:1˜10:1) to yield 4-(3-methoxycarbonylthiophen-2-yl)-3,6-dihydro-2H-pyridine-1-carboxylic acid t-butyl ester (4.2 g) as a yellow solid.
A solution of 4-(3-methoxycarbonylthiophen-2-yl)-3,6-dihydro-2H-pyridine-1-carboxylic acid t-butyl ester (4.2 g, 13 mmol, 1.0 eq.) and Pd(OH)₂/C (1.0 g, 1.4 mmol, 0.1 eq.) in MeOH (150 mL) was degassed and purged with hydrogen (3×). The mixture was hydrogenated (1 atm) at room temperature overnight, then the vessel was evacuated and purged with nitrogen, and the material was filtered and washed with MeOH (100 mL). The filtrate was concentrated then again allowed to hydrogenate at 1 atm at room temperature overnight. Filtering again as above, and concentrating the filtrate provided 4-(3-methoxycarbonylthiophen-2-yl)piperidine-1-carboxylic acid t-butyl ester (3.2 g) as a white solid.
A solution of 4-(3-methoxycarbonylthiophen-2-yl)piperidine-1-carboxylic acid t-butyl ester (3.8 g, 12 mmol, 1.0 eq.) in THF (70 mL) was degassed and purged with nitrogen (2×). Borane dimethyl sulfide complex (45 mL, 90 mmol, 110 eq.) was added at room temperature and the mixture was heated to reflux for 3 hours. The mixture was allowed to cool to room temperature and MeOH (20 mL) was slowly added. The mixture was then concentrated under vacuum. The addition of MeOH (20 mL) and concentrating was repeated two additional times. The crude material was applied onto a silica gel column and purified by chromatography with PE/EtOAc (10:1). The product was then purified by preparative HPLC to yield the title compound (1.8 g) as a white solid.

Example 30

4-(3-Phenoxymethylthiophen-2-yl)piperidine

PPh₃(28.9 mg, 0.1 mmol, 1.1 eq.) and 4-(3-Hydroxymethyl-thiophen-2-yl)piperidine-1-carboxylic acid t-butyl ester (29.7 mg, 0.1 mmol; 1.0 eq.) were dissolved in toluene (533 μL). Phenol (9.4 mg, 0.1 mmol, 1.0 eq.) was added, followed by the addition of DIAD (23.6 μL, 0.1 mmol, 1.2 eq.) in toluene (5 mL). The mixture was heated at 80° C. for 4 hours, then concentrated using an evaporator. The material was then dissolved in 1.25M HCl in EtOH (1 mL), and stirred at room temperature overnight. The material was again concentrated using an evaporator, then dissolved in 1:1 AcOH:H₂O, filtered, and purified by preparative HPLC to yield the title compound as a TFA salt (1.9 mg, 96% purity). MS m/z: [M+H]⁺ calcd for C₁₆H₁₉NOS, 274.12. found 274.2.

Example 31

Following the procedures described in the Schemes and/or the examples above, and substituting the appropriate starting materials and reagents, compounds 31-1 to 31-5, having formula Ij′, were also prepared:


	(Ij′)

MS m/z: [M + H]⁺

Ex.	R²	R³	R⁴	R⁵	R⁶	Formula	calcd	found

1	F	H	H	H	H	C₁₆H₁₈FNOS	292.11	292.2
2	H	Cl	H	H	H	C₁₆H₁₈ClNOS	308.08	308.0
3	F	H	F	H	F	C₁₆H₁₆F₃NOS	328.09	328.2
4	F	H	Cl	H	F	C₁₆H₁₆ClF₂NOS	344.06	344.0
5	F	H	H	F	Cl	C₁₆H₁₆ClF₂NOS	344.06	344.0

Example 36

Following the procedures described in the Schemes and/or the examples above, and substituting the appropriate starting materials and reagents, compounds 36-1 to 36-31, having formula Ij″, can also be prepared:


	(Ij″)

Ex.	R^1a	R^1b	R²	R³	R⁴	R⁵	R⁶

1	H	H	F	H	H	H	F
2	H	H	F	H	H	H	Cl
3	H	H	F	H	H	H	Br
4	H	H	F	F	H	H	F
5	H	H	F	Cl	H	H	F
6	H	H	F	H	Br	H	Cl
7	H	H	F	H	F	H	Cl
8	H	H	F	Me	H	H	F
9	F	H	F	H	H	H	H
10	H	F	F	H	H	H	H
11	F	H	F	H	H	H	F
12	H	F	F	H	H	H	F
13	F	H	F	H	H	H	Cl
14	H	F	F	H	H	H	Cl
15	F	H	F	F	H	H	F
16	H	F	F	F	H	H	F
17	F	H	F	H	F	H	F
18	H	F	F	H	F	H	F
19	F	H	F	H	H	F	Cl
20	H	F	F	H	H	F	Cl
21	—CF₃	H	F	H	H	H	Cl
22	H	—CF₃	F	H	H	H	Cl
23	—CF₃	H	F	F	H	H	F
24	H	—CF₃	F	F	H	H	F
25	—CF₃	H	F	H	F	H	F
26	H	—CF₃	F	H	F	H	F
27	F	F	F	H	H	H	F
28	F	F	F	H	H	H	Cl
29	F	F	F	H	F	H	F
30	F	F	F	F	H	H	F
31	F	F	F	H	H	F	Cl

Assay 1

hSERT, hNET, and hDAT Binding Assays

Membrane radioligand binding assays were used to measure inhibition of labeled ligand (³H-citalopram or ³H-nisoxetine or ³H-WIN35428) binding to membranes prepared from cells expressing the respective human recombinant transporter (hSERT or hNET or hDAT) in order to determine the pK_ivalues of test compounds at the transporters.

Membrane Preparation from Cells Expressing hSERT, hNET, or hDAT

Recombinant human embryonic kidney (HEK-293) derived cell lines stably transfected with hSERT or hNET, respectively, were grown in DMEM medium supplemented with 10% dialyzed FBS (for hSERT) or FBS (for hNET), 100 μg/ml penicillin, 100 μg/ml streptomycin, 2 mM L-glutamine and 250 μg/ml of the aminoglycoside antibiotic G418, in a 5% CO₂humidified incubator at 37° C. When cultures reached 80% confluence, the cells were washed thoroughly in PBS (without Ca²⁺ and Mg²⁺) and lifted with 5 mM EDTA in PBS. Cells were pelleted by centrifugation, resuspended in lysis buffer (10 mM Tris-HCl, pH7.5 containing 1 mM EDTA), homogenized, pelleted by centrifugation, then resuspended in 50 mM Tris-HCl, pH 7.5 and 10% sucrose at 4° C. Protein concentration of the membrane suspension was determined using a Bio-Rad Bradford Protein Assay kit. Membranes were snap frozen and stored at −80° C. Chinese hamster ovary membranes expressing hDAT (CHO-DAT) were purchased from PerkinElmer and stored at −80° C.

Binding Assays

Binding assays were performed in a 96-well assay plate in a total volume of 200 μL assay buffer (50 mM Tris-HCl, 120 mM NaCl, 5 mM KCl, pH 7.4) with 0.5, 1, and 3 μg membrane protein, for SERT, NET and DAT, respectively. Saturation binding studies, to determine radioligand K_dvalues for ³H-citalopram, ³H-nisoxetine, or ³H-WIN35428, respectively were conducted using 12 different radioligand concentrations ranging from 0.005-10 nM (³H-citalopram); 0.01-20 nM (³H-nisoxetine) and 0.2-50 nM (³H-WIN35428). Inhibition assays for determination of pK_ivalues of test compounds were conducted with 1.0 nM ³H-citalopram, 1.0 nM ³H-nisoxetine or 3.0 nM ³H-WIN35428, at 11 different concentrations of test compound ranging from 10 pM to 100 μM.
Stock solutions (10 mM in DMSO) of test compound were prepared and serial dilutions made using Dilution Buffer (50 mM Tris-HCl, 120 mM NaCl, 5 mM KCl, pH 7.4, 0.1% BSA, 400 μM ascorbic acid). Non-specific radioligand binding was determined in the presence of 1 μM duloxetine, 1 μM desipramine or 10 μM GBR12909 (each in Dilution Buffer) for the hSERT, hNET or hDAT assays, respectively.
Following a 60 minute incubation at 22° C. (or a period sufficient to reach equilibrium), the membranes were harvested by rapid filtration over a 96-well UniFilter GF/B plate, pretreated with 0.3% polyethyleneimine, and washed 6 times with 300 μl wash buffer (50 mM Tris-HCl, 0.9% NaCl, pH 7.5 at 4° C.). Plates were dried overnight at room temperature, ˜45 μl of MicroScint™-20 (Perkin Elmer) added and bound radioactivity quantitated via liquid scintillation spectroscopy. Inhibition curves and saturation isotherms were analyzed using GraphPad Prism Software package (GraphPad Software, Inc., San Diego, Calif.). IC₅₀values were generated from concentration response curves using the Sigmoidal Dose Response (variable slope) algorithm in Prism GraphPad. K_dand B_maxvalues for the radioligand were generated from saturation isotherms using the Saturation Binding Global Fit algorithm in Prism GraphPad. pK_i(negative decadic logarithm of K_i) values for test compounds were calculated from the best-fit IC₅₀values, and the K_dvalue of the radioligand, using the Cheng-Prusoff equation (Cheng & Prusoff (1973) Biochem. Pharmacol. 22(23):3099-3108): K_i=IC₅₀/(1+[L]/K_d), where [L]=concentration radioligand.
Compounds of the invention were tested in this assay and found to exhibit SERT and NET pK_ivalues as follows:


Ex.	SERT pK_i	NET pK_i

1	≧7.0	≧7.0
2-1	≧5.0	≧6.0
2-2	≧6.0	≧7.0
2-3	≧7.0	≧5.0
2-4	≧6.0	≧7.0
2-5	≧7.0	≧7.0
2-6	≧7.0	≧7.0
2-7	≧7.0	≧6.0
2-8	≧7.0	≧6.0
2-9	≧7.0	≧6.0
5	≧7.0	≧7.0
6-1	≧6.0	≧5.0
6-2	≧7.0	≧6.0
6-3	≧7.0	≧5.0
6-4	≧7.0	≧7.0
6-5	≧7.0	≧7.0
6-6	≧7.0	≧6.0
6-7	≧7.0	≧7.0
6-8	≧7.0	≧6.0
6-9	≧7.0	≧7.0
9	≧7.0	≧7.0
10-1	≧6.0	≧6.0
10-2	≧7.0	≧7.0
10-3	≧7.0	≧7.0
10-4	≧7.0	≧7.0
10-5	≧7.0	≧7.0
10-6	≧7.0	≧6.0
10-7	≧7.0	≧7.0
10-8	≧7.0	≧7.0
10-9	≧7.0	≧7.0
13	≧7.0	≧7.0
14-1	≧6.0	≧6.0
14-2	≧6.0	≧7.0
14-3	≧7.0	≧5.0
14-4	≧6.0	≧7.0
14-5	≧7.0	≧7.0
14-6	≧7.0	≧7.0
14-7	≧7.0	≧7.0
14-8	≧7.0	≧7.0
14-9	≧7.0	≧7.0
17	n.d.	n.d.
18-1	n.d.	n.d.
18-2	n.d.	n.d.
18-3	n.d.	n.d.
21	n.d.	n.d.
22-1	n.d.	n.d.
22-2	n.d.	n.d.
22-3	n.d.	n.d.
22-4	n.d.	n.d.
24	≧7.0	≧7.0
25-1	≧7.0	≧5.0
25-2	≧6.0	≧6.0
25-3	≧7.0	≧5.0
25-4	≧6.0	≧6.0
25-5	≧7.0	≧5.0
27	≧7.0	≧7.0
28-1	≧7.0	≧7.0
28-2	≧7.0	≧6.0
28-3	≧7.0	≧7.0
28-4	≧7.0	≧7.0
28-5	≧7.0	≧7.0
30	≧7.0	≧7.0
31-1	≧6.0	≧7.0
31-2	≧7.0	≧7.0
31-3	≧7.0	≧7.0
31-4	≧7.0	≧7.0
31-5	≧7.0	≧6.0

n.d = not determined

Assay 2

hSERT, hNET, and hDAT Neurotransmitter Uptake Assays

Neurotransmitter uptake assays were used to measure inhibition of ³H-serotonin (³H-5-HT), ³H-norepinephrine (³H-NE), and ³H-dopamine (³H-DA) uptake into cells expressing the respective transporter (hSERT, hNET or hDAT) in order to determine the pIC₅₀values of test compounds at the transporters.

³H-5-HT, ³H-NE, and ³H-DA Uptake Assays

HEK-293 derived cell lines stably-transfected with hSERT, hNET, or hDAT, respectively, were grown in DMEM medium supplemented with 10% dialyzed FBS (for hSERT) or FBS (for hNET and hDAT), 100 μg/ml penicillin, 100 μg/ml streptomycin, 2 mM L-glutamine and 250 μg/ml of the aminoglycoside antibiotic G418 (for hSERT and hNET) or 800 ug/ml (for hDAT), in a 5% CO₂humidified incubator at 37° C. When cultures reached 80% confluence, the cells were washed thoroughly in PBS (without Ca²⁺ and Mg²⁺) and lifted with 5 mM EDTA in PBS. Cells were harvested by centrifugation at 1100 rpm for 5 minutes, washed once by resuspension in PBS, then centrifuged. The supernatant was discarded and the cell pellet resuspended, by gentle trituration, in room temperature Krebs-Ringer bicarbonate buffer containing HEPES (10 mM), CaCl₂(2.2 mM), ascorbic acid (200 μM) and pargyline (200 μM), pH 7.4. The final concentration of cells in the cell suspension was 7.5×10⁴cells/ml, 1.25×10⁵cells/ml, and 5.0×10⁴cells/ml for SERT, NET, and DAT cell lines, respectively.
Neurotransmitter uptake assays were performed in a 96-well assay plate in a total volume of 400 μL assay buffer (Krebs-Ringer bicarbonate buffer containing HEPES (10 mM), CaCl₂(2.2 mM), ascorbic acid (200 μM) and pargyline (200 μM), pH 7.4) with 1.5×10⁴and 2.5×10⁴cells, for SERT and NET, respectively Inhibition assays for determination of pIC₅₀values of test compounds were conducted with 11 different concentrations, ranging from 10 pM to 100 μM. Stock solutions (10 mM in DMSO) of test compound were prepared and serial dilutions prepared using 50 mM Tris-HCl, 120 mM NaCl, 5 mM KCl, pH 7.4, 0.1% BSA, 400 μM ascorbic acid. Test compounds were incubated for 30 minutes at 37° C. with the respective cells, prior to addition of radiolabeled neurotransmitter, ³H-5-HT (20 nM final concentration), ³H-NE (50 nM final concentration), or ³H-DA (100 nM final concentration). Non-specific neurotransmitter uptake was determined in the presence of 2.5 μM duloxetine or 2.5 μM desipramine (each in Dilution Buffer) for the hSERT, hNET, or hDAT assays, respectively.
Following a 10 minute incubation, at 37° C., with radioligand, the cells were harvested by rapid filtration over a 96-well UniFilter GF/B plate, pretreated with 1% BSA, and washed 6 times with 650 μl wash buffer (ice cold PBS). Plates were dried overnight at 37° C., ˜45 μl of MicroScint™-20 (Perkin Elmer) added and incorporated radioactivity quantitated via liquid scintillation spectroscopy. Inhibition curves were analyzed using GraphPad Prism Software package (GraphPad Software, Inc., San Diego, Calif.). IC₅₀values were generated from concentration response curves using the Sigmoidal Dose Response (variable slope) algorithm in Prism GraphPad.
Compounds of the invention that were tested in this assay or in a fluorescence-based assay as described in Tsuruda et al. (2010) Journal of Pharmacological and Toxicological Methods 61(2):192-204 (data indicated by an asterisk in the table), were found to have serotonin and norepinephrine reuptake inhibition pIC₅₀values as follows:


Ex.	SERT pIC₅₀	NET pIC₅₀

2-9	≧7.0*	≧7.0*
9	≧7.0	≧7.0
10-4	≧7.0	≧7.0
13	≧7.0	≧7.0
17	≧7.0*	≧7.0*
18-1	≧7.0*	≧4.5*
18-2	≧7.0*	≧7.0*
18-3	≧6.0*	≧5.0*
21	≧7.0*	≧7.0*
22-1	≧6.0*	≧7.0*
22-2	≧5.0*	≧6.0*
22-3	≧6.0*	≧6.0*
22-4	≧7.0*	≧7.0*
24	≧7.0*	≧7.0*
27	≧7.0	≧7.0
28-3	≧7.0	≧7.0
31-2	≧7.0*	≧7.0*
31-3	≧7.0*	≧7.0*

Assay 3

Ex Vivo SERT and NET Transporter Occupancy Studies

Ex vivo radioligand binding and neurotransmitter uptake assays are used to determine the in vivo occupancy of SERT and NET, in selected brain regions, following in vivo administration (acute or chronic) of test compounds. Following administration of test compound (by intravenous, intraperitoneal, oral, subcutaneous or other route) at the appropriate dose (0.0001 to 100 mg/kg), rats (≧n=4 per group) are euthanized at specific time points (10 minutes to 48 hours) by decapitation and the brain dissected on ice. Relevant brain regions are dissected, frozen and stored at −80° C. until use.

Ex Vivo SERT and NET Radioligand Binding Assays

For ex vivo radioligand binding assays, the initial rates of association of SERT (³H-citalopram), and NET-(³H-nisoxetine) selective radioligands with rat brain crude homogenates, prepared from vehicle and test compound-treated animals, are monitored (see Hess et al. (2004) J. Pharmacol. Exp. Ther. 310(2):488-497). Crude brain tissue homogenates are prepared by homogenizing frozen tissue pieces in 0.15 mL (per mg wet weight) of 50 mM Tris-HCl, 120 mM NaCl, 5 mM KCl, pH 7.4 buffer. Radioligand association assays are performed in a 96-well assay plate in a total volume of 200 μl assay buffer (50 mM Tris-HCl, 120 mM NaCl, 5 mM KCl, 0.025% BSA, pH 7.4) with 650 μg wet weight tissue (equivalent to 25 μg protein). Homogenates are incubated for up to 5 minutes with ³H-citalopram (3 nM) and ³H-nisoxetine (5 nM), respectively, prior to termination of the assay by rapid filtration over a 96-well UniFilter GF/B plate, pretreated with 0.3% polyethyleneimine. Filters then are washed 6 times with 300 μl wash buffer (50 mM Tris-HCl, 0.9% NaCl, pH 7.4 at 4° C.). Non-specific radioligand binding is determined in the presence of 1 μM duloxetine, or 1 μM despiramine, for ³H-citalopram or ³H-nisoxetine, respectively. The plates are dried overnight at room temperature, ˜45 μl of MicroScint™-20 (Perkin Elmer) is added, and bound radioactivity is quantitated via liquid scintillation spectroscopy. The initial rates of association of ³H-citalopram and ³H-nisoxetine are determined by linear regression using GraphPad Prism Software package (GraphPad Software, Inc., San Diego, Calif.). The average rate of radioligand association to brain tissue homogenates from vehicle-treated animals is determined. The % occupancy of test compounds is then determined using the following equation:
% occupancy=100×(1−(initial rate association for test compound-treated tissue/mean rate association for vehicle-treated tissue))
ED₅₀values are determined by plotting the log 10 of the dose of the test compound against the % occupancy. ED₅₀values are generated from concentration response curves using the Sigmoidal Dose Response (variable slope) algorithm in GraphPad Prism.

Ex Vivo SERT and NET Uptake Assays

Ex vivo neurotransmitter uptake assays, in which the uptake of ³H-5-HT or ³H-NE into rat brain crude homogenates, prepared from vehicle and test compound-treated animals, are used to measure in vivo SERT and NET transporter occupancy (see Wong et al. (1993) Neuropsychopharmacology 8(1):23-33). Crude brain tissue homogenates are prepared by homogenizing frozen tissue pieces in 0.5 mL (per mg wet weight) of 10 mM HEPES buffer pH 7.4, containing 0.32 M sucrose, 200 μM ascorbic acid and 200 μM pargyline, at 22° C. Neurotransmitter uptake assays are performed in a 96-well Axygen plate in a total volume of 350 μl assay buffer (Krebs-Ringer bicarbonate buffer with 10 mM HEPES, 2.2 mM CaCl₂, 200 μM ascorbic acid and 200 μM pargyline, pH 7.4) with 50 μg protein. Homogenates are incubated for 5 minutes at 37° C. with ³H-5-HT (20 nM) and ³H-NE (50 nM), respectively, prior to termination of the assay by rapid filtration over a 96-well UniFilter GF/B plate, pretreated with 1% BSA. Plates are washed 6 times with 650 μl wash buffer (ice cold PBS) and dried overnight at 37° C., prior to addition of ˜45 μL of MicroScint™-20 (Perkin Elmer). Incorporated radioactivity is quantitated via liquid scintillation spectroscopy. Non-specific neurotransmitter uptake is determined in parallel assays in which tissue homogenates are incubated with ³H-5-HT (20 nM) or ³H-NE (50 nM) for 5 minutes at 4° C.

Assay 4

Other Assays

Other assays that are used to evaluate the pharmacological properties of test compounds include, but are not limited to, cold ligand binding kinetics assays (Motulsky and Mahan (1984) Molecular Pharmacol. 25(1):1-9) with membranes prepared from cells expressing hSERT or hNET; conventional membrane radioligand binding assays using radiolabeled, for example, tritiated, test compound; radioligand binding assays using native tissue from, for example rodent or human brain; neurotransmitter uptake assays using human or rodent platelets; neurotransmitter uptake assays using crude, or pure, synaptosome preparations from rodent brain.

Assay 5

Formalin Paw Test

Compounds are assessed for their ability to inhibit the behavioral response evoked by a 50 μl injection of formalin (5%). A metal band is affixed to the left hind paw of male Sprague-Dawley rats (200-250 g) and each rat is conditioned to the band for 60 minutes within a plastic cylinder (15 cm diameter). Compounds are prepared in pharmaceutically acceptable vehicles and administered systemically (i.p., p.o.) at pre-designated times before formalin challenge. Spontaneous nociceptive behaviors consisting of flinching of the injected (banded) hind paw are counted continuously for 60 minutes using an automated nociception analyzer (UCSD Anesthesiology Research, San Diego, Calif.). Antinociceptive properties of test articles are determined by comparing the number of flinches in the vehicle and compound-treated rats (Yaksh et al., “An automated flinch detecting system for use in the formalin nociceptive bioassay” (2001) J. Appl. Physiol. 90(6):2386-2402).

Assay 6

Spinal Nerve Ligation Model

Compounds are assessed for their ability to reverse tactile allodynia (increased sensitivity to an innocuous mechanical stimulus) induced by nerve injury. Male Sprague-Dawley rats are surgically prepared as described in Kim and Chung “An experimental model for peripheral neuropathy produced by segmental spinal nerve ligation in the rat” (1992) Pain 50(3):355-363. Mechanical sensitivity is determined as the 50% withdrawal response to innocuous mechanical stimuli (Chaplan et al., “Quantitative assessment of tactile allodynia in the rat paw” (1994) J. Neurosci. Methods 53(1):55-63) before and after nerve injury. One to four weeks post-surgery, compounds are prepared in pharmaceutically acceptable vehicles and administered systemically (i.p., p.o.). The degree of nerve injury-induced mechanical sensitivity before and after treatment serves as an index of the compounds' antinociceptive properties.
While the present invention has been described with reference to specific aspects or embodiments thereof, it will be understood by those of ordinary skilled in the art that various changes can be made or equivalents can be substituted without departing from the true spirit and scope of the invention. Additionally, to the extent permitted by applicable patent statues and regulations, all publications, patents and patent applications cited herein are hereby incorporated by reference in their entirety to the same extent as if each document had been individually incorporated by reference herein.

Claims

1. A compound of formula I:

where: X is —N—; HAr is a 5-7 membered heteroaryl with one or two heteroatoms selected from nitrogen, oxygen, and sulfur; a is 0 or 1; R¹is halo or trifluoromethyl; and R^2-6are independently hydrogen, halo, —C_1-6alkyl, —CF₃, —C_2-6alkynyl, —O—C_1-6alkyl, —OCF₃, —C_1-4alkylene-O—C_1-4alkyl, —C_0-1alkylene-phenyl, —O—C_0-3alkylene-phenyl, —C_0-6alkylene-OH, —CN, —COOH, —CHO, —C(O)—C_1-6alkyl, —C(O)—C_1-4alkyl, —CH₂SH, —S—C_1-6alkyl, —C_1-4alkylene-S—C_1-4alkyl, or —NO₂; wherein the phenoxymethyl group and the piperazine ring are attached to adjacent carbon atoms on HAr; or a pharmaceutically acceptable salt thereof.

2-4. (canceled)

5. The compound of claim 1, where R²is hydrogen or halo.

6. The compound of claim 1, where R³is hydrogen or halo.

7. The compound of claim 1, where R⁴is hydrogen or halo.

8. The compound of claim 1, where R⁵is hydrogen or halo.

9. The compound of claim 1, where R⁶is hydrogen or halo.

10. The compound of claim 1, where a is 0.

11. The compound of claim 1, where HAr is selected from 2-pyridyl, 3-pyridyl, 4-pyridyl, 5-pyridyl, 2-furyl, 4-furyl, 2-thienyl, 3-thienyl, and 4-thienyl.

12. The compound of claim 11, where R^2-6are independently hydrogen or halo.

13-14. (canceled)

15. A method of preparing the compound of claim 1, the process comprising deprotecting a compound of formula IV:

or a salt thereof, where P represents an amino-protecting group, to provide a compound of formula I.

16. The method of claim 15, where a is 0; HAr is selected from 2-pyridyl, 3-pyridyl, 4-pyridyl, 5-pyridyl, 2-furyl, 4-furyl, 2-thienyl, 3-thienyl, and 4-thienyl; and R^2-6are independently hydrogen or halo.

17. A pharmaceutical composition comprising the compound of claim 1 and a pharmaceutically acceptable carrier.

18-20. (canceled)