US20120112093A1 - System and method for measuring depolarization - Google Patents
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- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
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Definitions
- Application ARL 04-11 entitled “SYSTEM AND METHOD FOR DETERMINING THREE-DIMENSIONAL INFORMATION FROM TWO-DIMENSIONAL IMAGES,” invented by Ronald Meyers, David Rosen, and Keith Deacon
- Application ARL 04-12 entitled “SYSTEM AND METHOD FOR DETERMINING THREE-DIMENSIONAL INFORMATION FROM PHOTOEMISSION INTENSITY DATA,” invented by Ronald Meyers and David Rosen, both of which are filed on even date herewith and hereby incorporated by reference.
- the present invention relates to methods and apparatus to determine the depolarization of electromagnetic waves passing through an environment.
- the present invention relates to the determination of the depolarization efficiency of the atmosphere, using the dependence of photoexcited emission (photoluminescence emission, Raman scatter, or diffuse reflection) of a reference object.
- Raman scattering is the inelastic scattering of a photon. When light is scattered from an atom or molecule, most photons are elastically scattered (Rayleigh scattering), such that the scattered photons have the same energy (frequency) and wavelength as the incident photons. However, a small fraction of the scattered light (approximately 1 in 10 million photons) is scattered by an excitation, with the scattered photons having a frequency different from, and usually lower than, the frequency of the incident photons.
- Raman scattering can occur with a change in vibrational, rotational or electronic energy of a molecule (see energy level).
- Chemists are concerned primarily with the vibrational Raman Effect.
- Depolarization efficiency is the fractional change in degree of polarization per unit length for electromagnetic radiation propagating through an environment, such as the atmosphere. Measurement of depolarization efficiency would improve imaging of remote objects through environments.
- the environments include atmospheric environments and underwater environments.
- Depolarization of scattered laser radiation has been measured remotely using depolarization lidar.
- Depolarization lidar has been used to study nonspherical dust particles in the atmosphere, and multiscattering in clouds.
- a laser beam is emitted from the lidar system, passes through the atmosphere, backscatters off an atmospheric particle, and passes through the atmosphere again, and is collected in a detection device in the lidar which determines its polarization state.
- the scattering processes that affect the depolarization measured by lidar include both forward scattering processes and backscattering processes.
- the radiation that comes back to the lidar system has to be backscattered from an atmospheric particle at least once, and the impact of this single scattering process may or may not be greater than the forward scattering processes that depolarize the laser beam.
- the depolarization of radiation in the atmosphere biases polarimetric sensors.
- the application of depolarization lidar would be extended if the forward and backward scattering processes could be separately determined.
- Some methods that may discriminate forward scattering processes from backscattering process requires either a one way path for the laser beam or a well characterized reflector a known distance from the laser and receiver. Both types of measurements are biased by receivers more sensitive to one polarization state over the others.
- a technique is provided that uses a photoexcited emitter (i.e., photoluminescent or Raman scattering material) with specular reflection to replace the reflector in methods where the laser and reflector are near each other.
- a photoexcited emitter i.e., photoluminescent or Raman scattering material
- specular reflection i.e., specular reflection
- One advantage provided over conventional depolarization methods is that the photoemitted radiation is almost completely randomized. Therefore, the polarization bias of the receiver cannot impact the measurements.
- Another advantage is that the photoemission radiance of the photoemitter can be varied with incident polarization without an optical element. Therefore, optical elements that may have an uncharacterized birefringence are not required at the target.
- An apparatus for determining the depolarization efficiency of an environment where the environment is the atmosphere or water, and includes a transmitter, a receiver, and signal processing circuitry, for example, a computer.
- a reference object, or reference is located within the environment at a reference distance from the apparatus.
- the transmitter includes a radiation source, such as a laser, providing incident radiation that is directed to an incidence point on a reference.
- the incident radiation has an initial polarization as it enters the environment, and the returned radiation photoemission radiance that varies with the polarization state of the transmitter beam near the target and just before it is incident on the reference.
- the transmitter illustratively includes a laser or lamp source inclusive of a polarizer or polarization modulator, and, optionally, a filter that selectively transmits incident radiation.
- the receiver receives returned radiation from the reference, and determines the radiance of the photoexcited radiation.
- the receiver includes a detector, such as a camera, and may also include an analyzer and a filter that selectively transmits the returned radiation of interest.
- the returned radiation may be diffuse reflection, in which case the receiver may require a polarizer to filter out the specular reflection.
- the returned radiation may be photoluminescence (including fluorescence) or Raman scattering, and optionally has a wavelength significantly different from the incident radiation.
- the receiver optionally includes a filter that transmits the photoluminescence while blocking radiation at the incident radiation wavelength.
- the shape and optical properties of the reference object are known, the distance between source/ receiver system, and reference object are known, but the depolarization strength of the environment between source/receiver system and reference object are unknown and must be calculated.
- the signal processing circuitry such as a computer, is able to calculate the depolarization efficiency of the environment from the initial polarization, final polarization, and the reference distance.
- the circuitry receives detector data from the detector, the detector data being correlated with the intensity of returned radiation.
- the apparatus optionally includes timing electronics, to allow detector data to be correlated with initial polarization, the timing electronics being in electronic communication with the polarization modulator and with the detector.
- the apparatus may receive the reference distance from an associated device with which the apparatus is in communication.
- an asphalt road could be a photoluminescent target that is already in the environment.
- a plastic target of known shape and composition could be introduced in the environment as a target for atmospheric studies. In either case, the shape and orientation of the target is what makes the intensity of emission dependent on transmitter polarization state.
- the depolarization strength of the medium can be determined using the object distance, the initial polarization of the incident radiation, the orientation and shape of the object, and the variation of the radiance of the received radiation with the polarization of the transmitted radiation.
- FIG. 1 shows a schematic of an apparatus used to determine depolarization efficiency of an environment using photoluminescence measurements and temporal variations in receiver intensity.
- FIG. 2 shows a schematic of an apparatus used to determine depolarization efficiency of an environment using photoluminescence measurements and spatial variations in receiver intensity.
- the present invention has utility as part of a remote imaging method, enabling the remote imaging method to compensate for image distortion associated with light passing through a distorting environment.
- a method is provided according to the present invention for measuring depolarization efficiency using a polarimetric stereo approach.
- a volumetric (i.e., three-dimensional) object with known properties is acquired as a reference object (or reference), illuminated with an electromagnetic radiation source that is completely polarized and at a known distance from the reference, and imaged using either photoluminescence or diffuse reflectance from the reference.
- the variations in radiance intensity at the receiver is measured and correlated to the polarization of the electromagnetic source. From the variations of emission radiance at the receiver, the degree of depolarization between the electromagnetic source and the receiver can be calculated using the correlation.
- the depolarization efficiency is the fraction of polarized radiation depolarized in a unit distance.
- An inventive method uses the variations in pixel intensity to calculate the depolarization without any temporal variation of laser polarization.
- the detector is a camera, where the intensity of received radiation is measured at each pixel.
- the polarization-averaged intensity at each pixel is proportionate to the depolarization of the object at each corresponding object point, where the transmittance at each object point is a function of both the polarization state of the incident radiation at the object surface and the angle of incidence at that object point.
- the three-dimensional shape of the target is known, so the depolarization of the surface of the reference-object can be calculated at each point.
- the variations in radiance intensity at each pixel of the receiver is measured and correlated to the polarization of the electromagnetic source.
- the depolarization can be calculated by dividing the measured polarization of the return radiation by the depolarization of the surface.
- the distance-averaged depolarization efficiency of the atmosphere between the object and the radiation source is found from the difference in degree of polarization between the radiation source and the object surface, divided by the distance to the reference.
- the geometric shape of the reference can be chosen to present a curved surface, such as a circular cross-section, to the incident light source.
- the reference can be a cylinder.
- the radiance values along the axis vary less with polarization than radiance values around the circumference.
- the composition, at least of a surface layer of the reference, can be chosen to present desired optical properties, such as photoluminescence or diffuse reflection, as described in more detail below.
- An exemplary inventive apparatus for determining the depolarization efficiency of an environment includes a transmitter, inclusive of a radiation source that provides the incident radiation, and a receiver, inclusive of a radiation detector.
- the radiation detector is optionally a laser
- the transmitter optionally includes a polarizer (a fixed polarizer or polarization modulator), and the transmitter optionally includes a camera and an analyzer.
- An exemplary analyzer includes a fixed polarizer with a polarization transmission detection orthogonal to that of a fixed polarizer in the transmitter.
- Apparatus and methods according to the present invention are readily used to determine the depolarization efficiency of an environment in which the reference is located.
- the incident radiation passes through the environment on the way to the object. Determination of environmental depolarization efficiency is critical to improving remote object imaging and tracking through the environment and as such has applications in satellite, atmospheric, and oceanographic imaging.
- the environment is the atmosphere
- the incident radiation is depolarized by atmospheric particles. Therefore, if the shape of the reference is known independently of the photoluminescent image, then the depolarization efficiency of the atmosphere between a polarized radiation source and the reference is calculated.
- the dependence of specular reflectivity on incident angle and polarization of the incident radiation is used to determine stereo information from radiation returned from the reference (such as the photoluminescence emission intensity, or diffuse reflectance).
- Apparatus according to embodiments of the present invention is also used to determine three-dimensional shape information of remote objects, and spectroscopic information relating to an object surface may also be also obtained.
- a source of electromagnetic radiation with time-varying differential-polarization provides incident radiation that illuminates a remote object.
- Photoexcited emission i.e., photoluminescence, Raman scattering, or diffuse reflection
- time variations in photoluminescence intensity or emission intensity
- stereo vision information i.e. three-dimensional information
- Photoluminescence spectra regardless of whether the spectra are excitation and emission based, contain chemical information on the composition of points on the object.
- the difference between emission intensities at two polarization states, divided by the time-averaged emission intensity, is sensitive to the three-dimensional contours of the object. Therefore, the radiance of emission measured at the receiver is sensitive both to the three-dimensional contours of the object and to the orientation of the object.
- the difference between photoluminescence intensities at the two incident radiation polarizations varies between one angle of incidence and another, hence varies at different incident points on a curved reference surface.
- the polarimetric photoluminescence signal is a function of time variations in emission intensity.
- the difference in photoluminescence intensities at two polarization states divided by the polarization-averaged photoluminescence intensity is designated the degree of polarization of the polarimetric photoluminescence signal.
- the degree of polarization of the polarimetric photoluminescence signal differs from the degree of polarization of emitted photoluminescence, which describes the polarization of emitted radiation.
- the specular reflectance of the object varies with the polarization of the incident radiation, because incident radiation that is removed by specular reflection is not available to become photoluminescence emission, as described in Miyazaki et al., “Determining surface orientations of transparent objects based on polarization degrees in visible and infrared wavelengths,” J. Opt. Soc. Am. A 19, 687-694 (2004).
- the emission intensity is anti-correlated with the specular reflectance due to radiative transfer, related to the conservation of energy. Therefore, if the incident angle is greater than zero but less than a critical angle, and if the total intensity of incident radiation is constant in time, this intensity of emission will vary with the polarization of the incident radiation.
- the photoluminescence carries information on the three-dimensional shape of the object, because specular reflectance varies with incident angle as well as incident polarization. Surface curvature and changes in angle of incidence are thus embedded in the time variation of emission intensity.
- the response of the photoluminescence to incident radiation can be used to determine the strength of depolarization of the medium (i.e., atmosphere or water). Therefore, if the object has a curved or bent surface, the photoluminescence across the surface varies with position.
- This invention concerns the use of polarization measurements in photoexcited emission or diffuse reflection to determine depolarization of the medium.
- the emission intensity varies with the linear polarization of the incident radiation.
- the Fresnel equations govern the specular reflectance of dielectric materials and indicate that the reflectance of s-polarized radiation is greater than reflectance of p-polarized radiation.
- FIG. 1 shows a schematic of atmospheric depolarization efficiency measured by using a reference having photoluminescence, and incident radiation having time-varying degree of initial polarization.
- Electromagnetic radiation from a radiation source 10 passes through a polarization modulator 12 and excitation wavelength filter 14 .
- the resulting incident radiation then passes through an environment, in this example the atmosphere, and is incident on the outer surface of a reference object (or more concisely, a reference) 16 .
- FIG. 1 shows the incident radiation, I, incident at an incidence point 18 on the reference 16 , and also shows a dashed line representing the tangent to the surface at the incidence point.
- a specular reflection beam is shown as R, and a transmitted beam (T) is transmitted into the reference and may be completely absorbed.
- Photoluminescence emission P induced by the incident radiation, is collected by a lens 20 , passes through emission wavelength filter 22 , and is incident on a detector 24 , in this example a camera.
- the wavelength filter 22 operates to separate the photoluminescence from reflected light.
- Signal processing circuitry 26 is in communication with the polarization modulator 12 and detector 24 , so that detected photoluminescence intensity is correlated with incident polarization.
- the detector 24 is illustratively a camera producing an intensity image matrix for photoluminescence. Three-dimensional data relating to the object may be shown on display 28 .
- the incident radiation I has time-varying differential polarization, P o (t), where P o is an initial degree of polarization (before passing through the environment) and t is time, and constant polarization-averaged intensity.
- P o is an initial degree of polarization (before passing through the environment) and t is time, and constant polarization-averaged intensity.
- the incident radiation passes through the environment, in this example the atmosphere, where it is partially depolarized by atmospheric particles.
- the radiation incident on the surface of the reference has a degree of polarization P(t+y/c), where P(t) is degree of polarization on the reference surface, t is time that the laser pulse is emitted and y is the distance between laser and surface.
- the reference is preferably an object having a known shape, such as a cylinder or sphere, or other shape having a known cross-section or a shape that may be approximated.
- the reference has a surface that produces photoluminescence on excitation by the incident radiation.
- the reference may have an outer layer, such as a coating containing a luminescent substance.
- a laser radiation source operating at a wavelength that excites photoluminescence from the surface reference is well suited for this exemplary inventive methodology.
- Photoluminescence emission is generated that has a radiance that varies with time and angle of incidence.
- the depth of modulation i.e., emission intensity for one polarization state of excitation minus the emission state for the orthogonal state of polarization
- P(t) degree of polarization
- the time electronics stores the initial polarization P 0 (t) and the fractional change in emission intensity P(t), and from this the total depolarization factor P/P 0 is calculated. From the distance, y, over which the depolarization occurs, the path-averaged depolarization efficiency can be calculated.
- FIG. 2 shows the reference is a known three-dimensional shape, such as a cylinder or sphere, having an outer surface comprising a material producing both diffuse and specular reflectance at the wavelength, ⁇ of the incident radiation.
- the electromagnetic radiation source in this example is a laser having a laser emission wavelength and in this example the radiance of photoexcited emission or diffuse reflected radiation is measured using a laser beam having a constant (time-independent) intensity and polarization.
- photoexcited emission photoluminescence or Raman
- no polarizer is necessary on the camera receiver end because the photoexcited radiation is at a different wavelength than the transmitter radiation (i.e., ⁇ i ⁇ e ).
- the narrow band filters on source and camera both pass the wavelength and absorb other wavelengths.
- the path-averaged depolarization efficiency can be calculated using the distance, y, over which depolarization occurs in the environment, in this case between the radiation source and the reference.
- the laser beam is fully polarized in the vertical direction.
- Diffuse reflection (scattered radiation) from the reference is imaged and filtered by the analyzer 54 set orthogonal to the polarization of the laser.
- the analyzer has a horizontal orientation (out of the plane of the paper) so that the specular reflection is blocked.
- an analyzer is a polarizer within the receiver. From the variation of intensity of the diffuse reflectance on the image, the polarization state of the incident radiation can be calculated. Using the polarization state on the object, and the distance y between object and source, the depolarization efficiency ⁇ can be calculated.
- ⁇ is the depolarizance of the surface of the reference object
- m is the index of refraction of the object
- ⁇ is an angle of transmitter polarization
- ⁇ is the angle of incidence
- ⁇ is the azimuthal angle.
- the depolarization efficiency, D can be determined by the following equation,
- ⁇ 0 cos 2 ( ⁇ arccos( m ⁇ 1 sin ⁇ )),
- P is calculated differently for the two cases in FIG. 1 and FIG. 2 .
- the measured luminescence in both cases is described by a function L( ⁇ ; ⁇ , ⁇ ,y) where L is the luminescence intensity.
- ⁇ varies with time, t.
- the intensity L is measured at various times, t.
- the other angles ( ⁇ and ⁇ ) are fixed during the determination.
- the polar angle, ⁇ must be known previous to the determination.
- the polarization for this case is,
- the angle ⁇ may be known before the determination of D. However, a variation on the technique would be that the angle ⁇ be determined from the values measured of L at various times, t. In the case of FIG. 2 , ⁇ varies with the position on the object. The other angles ( ⁇ and ⁇ ) are fixed during the determination. The angles, ⁇ and ⁇ , must be known and fixed to the determination. The polarization for this case is,
- D can be calculated in the case of FIG. 1 if the polarization varies with time.
- D can be calculated in the case of FIG. 2 when the polarization is constant in time.
- the total polarization of the received light at distance z which includes the depolarization of the atmosphere, is P f
- the strength of depolarization, D, of the medium between transmitter and receiver is calculated using the following formula.
- the reference object may be a natural or man-made object.
- a reference may be positioned a certain distance from the apparatus by placement, firing a projectile, dropping from an aircraft, or other method, at the time of measurement or at some earlier time.
- the reference may include an electronic circuit or transponder, so as to facilitate determination of the reference object distance.
- a locator device may be placed on a natural object.
- the reference can be an object chosen to have desired properties, such as photoluminescence or diffuse scattering.
- the reference can be an object presenting a curved surface, for example providing a plurality of incident angles for incident radiation.
- the reference distance is the path length of the incident radiation through the environment to the point of incidence on the reference.
- the reference distance may be much larger than the dimensions of the apparatus used, and may also be much larger than the size of the reference object.
- the reference distance may be equivalently referred to as, for example, the distance between the radiation source and the center of the reference object, without any significant loss of accuracy.
- determining the position of the point of incidence on the reference object is used in order to confirm the path length.
- the reference distance may be determined by satellite imaging, a ranging device (such as laser ranging device, ultrasound ranging device, optical rangefinder, and the like), relative absolute position as determined using a GPS or similar device, from the angle subtended at the apparatus location (for an object of known size), apparent object position relative to known landmarks, or any other distance measurement method.
- the apparatus may include additional functionalities related to reference distance determination, such as ranging devices, wireless transceiver in communication with the reference (if appropriate), and the like.
- the radiation source is optionally a pulsed laser, so that ranging can be achieved using gating times.
- the reference distance can be determined using photometry, such as photometric stereo measurements using a color camera.
- the transmitter provides incident radiation having desired properties.
- the transmitter comprises at least one radiation source, such as a laser, and optionally includes additional components to modify the properties of the incident beam, such as one or more lenses, an aperture, a polarizer or polarization modulator, a filter, and the like.
- the transmitter also optionally includes beam steering components to select an incidence location on a reference.
- Other possible transmitters include lamps and light emitting diodes.
- the incident wavelength may be varied, for example by selecting one of a plurality of radiation sources, tuning a radiation source (such as a dye laser or wavelength adjustable semiconductor laser), or modifying or changing a filter.
- tuning a radiation source such as a dye laser or wavelength adjustable semiconductor laser
- the radiation source may be a directional source of electromagnetic radiation such as a laser providing laser radiation.
- the laser radiation is inherently polarized, or alternatively is polarized by an external polarizer.
- the laser radiation polarization is controllable by electrical signals applied to the laser, for example applied to an electrooptic material within the laser structure. Electrical signals are applied to a laser, such as a semiconductor laser structure, to control polarization and/or emission wavelength.
- a polarization modulator may be an electrooptic modulator, for example comprising an electrooptic crystal.
- the function of a polarization modulator is included in a laser.
- a laser such as a semiconductor laser, is optionally used having a laser radiation output having electrically controllable polarization properties.
- the receiver comprises the detector and any associated components, such as a lens or filter, are used to collect and detect the returned radiation.
- Returned radiation from the reference is collected by the receiver.
- the receiver includes a detector, such as an optical detector.
- the receiver is sensitive to at least one spectrum of: visible wavelengths, IR wavelengths (including near-IR and thermal radiation wavelengths), THz, GHz, or other electromagnetic radiation ranges.
- the receiver is sensitive to near-IR wavelengths and visible, or UV-visible ranges.
- a polarizer at the receiver end is used to eliminate specular reflected radiation from the receiver.
- the returned radiation also is optionally Raman scattering.
- Holographic filters in the receiver are used to selectively transmit Raman scattered radiation.
- Apparatus preferably includes signal processing circuitry, operational to determine the depolarization of the environment from the initial polarization, the final polarization, and the reference distance.
- the signal processing circuitry allows the detector signal to be correlated with the polarization modulator, if used, or equivalently with the polarization output of the radiation source if this is controllable.
- the signal processing circuitry operates on a computer, the computer including a processor, clock, memory, data input, and data output (such as a display driver coupled to a display or transmitter in communication with a network).
- the processor may execute one or more algorithms to provide the results of calculations described herein.
- examples of the present invention can be used to determine depolarization of various media, such as other gases, liquids (such as water, including river water and sea water), and solids, such as glasses.
- the transmitter and receiver may be disposed within a single housing, to form a unitary apparatus.
- the apparatus is carried or otherwise supported by a land vehicle, boat, submarine, aircraft, satellite, a person, or may be set on the ground, for example using a stand.
- the apparatus optionally includes a display, which may be used to provide a visual indication of determined data.
- the display also is useful to show the part of the environment towards which the incident radiation is directed, for example in combination with zoom or image enhancement capabilities, in order to direct the incident radiation onto the surface of the desired reference.
- a camera optionally is used to image the reference object, the same camera being used as a sensor in the receiver, and/or for photometric stereo measurements.
- the transmitter and receiver are contained in separate housings, and are in data communication through a cable or wireless communications link. It is appreciated that multiple transmitters and/or receivers optionally are used, for example to allow data to be collected for multiple angular positions, wavelengths, reference distances, and the like.
- An apparatus optionally provides for collection of other ambient condition data, such as temperature, pressure, humidity, other meteorological conditions such as cloud cover or precipitation, time, sun position, pollutant analysis, particulate form and concentration, altitude, position (for example, using a GPS), and the like. Atmospheric depolarization may be correlated with ambient conditions, and may be used to interpret data collected under similar conditions.
- ambient condition data such as temperature, pressure, humidity, other meteorological conditions such as cloud cover or precipitation, time, sun position, pollutant analysis, particulate form and concentration, altitude, position (for example, using a GPS), and the like.
- Atmospheric depolarization may be correlated with ambient conditions, and may be used to interpret data collected under similar conditions.
- An apparatus also optionally includes an operational mode selector, the operational mode selector having a first setting in which photoluminescence is detected, and a second setting in which diffuse reflection is detected.
- An apparatus also optionally includes a data input, which can be the data input of the computer, the data input receiving the object distance so as to allow determination of the depolarization efficiency.
- Ambient condition data is collected by instrumentation included within the device, received directly from other devices over a communications link, or may be entered by an apparatus operator using a data entry mechanism.
- the apparatus also optionally includes a housing, which in some examples may seal against ingress of the environment under test (for example, if the environment is water, such as seawater).
- the housing may be provided with one or more windows to allow radiation to enter and/or leave the housing.
- photoluminescence or “photoluminescent radition” refers to a process in which a substance absorbs photons (electromagnetic radiation) and then re-radiates photons. Quantum mechanically, the process can be described as an excitation to a higher energy state and then a return to a lower energy state accompanied by the emission of a photon.
- the photoluminescent processes include, but are not limited to, resonant radiations, in which a photon of a particular wavelength is absorbed and an equivalent photon is immediately emitted.
Abstract
Description
- The invention described herein may be manufactured, used, and licensed by or for the United States Government.
- This application is related to Application ARL 04-11 entitled “SYSTEM AND METHOD FOR DETERMINING THREE-DIMENSIONAL INFORMATION FROM TWO-DIMENSIONAL IMAGES,” invented by Ronald Meyers, David Rosen, and Keith Deacon, and Application ARL 04-12 entitled “SYSTEM AND METHOD FOR DETERMINING THREE-DIMENSIONAL INFORMATION FROM PHOTOEMISSION INTENSITY DATA,” invented by Ronald Meyers and David Rosen, both of which are filed on even date herewith and hereby incorporated by reference.
- The present invention relates to methods and apparatus to determine the depolarization of electromagnetic waves passing through an environment.
- The present invention relates to the determination of the depolarization efficiency of the atmosphere, using the dependence of photoexcited emission (photoluminescence emission, Raman scatter, or diffuse reflection) of a reference object. Raman scattering is the inelastic scattering of a photon. When light is scattered from an atom or molecule, most photons are elastically scattered (Rayleigh scattering), such that the scattered photons have the same energy (frequency) and wavelength as the incident photons. However, a small fraction of the scattered light (approximately 1 in 10 million photons) is scattered by an excitation, with the scattered photons having a frequency different from, and usually lower than, the frequency of the incident photons. In a gas, Raman scattering can occur with a change in vibrational, rotational or electronic energy of a molecule (see energy level). Chemists are concerned primarily with the vibrational Raman Effect. Depolarization efficiency is the fractional change in degree of polarization per unit length for electromagnetic radiation propagating through an environment, such as the atmosphere. Measurement of depolarization efficiency would improve imaging of remote objects through environments. The environments include atmospheric environments and underwater environments.
- Depolarization of scattered laser radiation has been measured remotely using depolarization lidar. Depolarization lidar has been used to study nonspherical dust particles in the atmosphere, and multiscattering in clouds. In depolarization lidar measurements, a laser beam is emitted from the lidar system, passes through the atmosphere, backscatters off an atmospheric particle, and passes through the atmosphere again, and is collected in a detection device in the lidar which determines its polarization state. The scattering processes that affect the depolarization measured by lidar include both forward scattering processes and backscattering processes. However, the radiation that comes back to the lidar system has to be backscattered from an atmospheric particle at least once, and the impact of this single scattering process may or may not be greater than the forward scattering processes that depolarize the laser beam. The depolarization of radiation in the atmosphere biases polarimetric sensors. The application of depolarization lidar would be extended if the forward and backward scattering processes could be separately determined.
- Some methods that may discriminate forward scattering processes from backscattering process requires either a one way path for the laser beam or a well characterized reflector a known distance from the laser and receiver. Both types of measurements are biased by receivers more sensitive to one polarization state over the others.
- One way methods have the disadvantage that both receiver and transmitter must be at a large distance from each other. Methods where the receiver and the laser are near each other have required a well characterized reflector and a known distance from the reflector. Well characterized types of reflector include retroreflector and dielectric mirror. However, reflectors often have a bias for one polarization or the other because of their intrinsic birefringence. Birefringence is the decomposition of a ray of light into two rays (the ordinary ray and the extraordinary ray) when it passes through certain types of material.
- Thus, there exists a need for a method of measuring the polarization state of the laser beam unbiased by receiver and by reflector would be convenient for depolarization studies near the ground. For example, the capability of distinguishing forward from backward scattering could be very useful in distinguishing the scattering of nonspherical dust particles from multiscattering of water droplets. There also exists a need for the ability to measure the forward scatter of a transmitter beam independently of the backscatter in real-world environments.
- A technique is provided that uses a photoexcited emitter (i.e., photoluminescent or Raman scattering material) with specular reflection to replace the reflector in methods where the laser and reflector are near each other. One advantage provided over conventional depolarization methods is that the photoemitted radiation is almost completely randomized. Therefore, the polarization bias of the receiver cannot impact the measurements. Another advantage is that the photoemission radiance of the photoemitter can be varied with incident polarization without an optical element. Therefore, optical elements that may have an uncharacterized birefringence are not required at the target.
- An apparatus for determining the depolarization efficiency of an environment is provided, where the environment is the atmosphere or water, and includes a transmitter, a receiver, and signal processing circuitry, for example, a computer. A reference object, or reference, is located within the environment at a reference distance from the apparatus. The transmitter includes a radiation source, such as a laser, providing incident radiation that is directed to an incidence point on a reference. The incident radiation has an initial polarization as it enters the environment, and the returned radiation photoemission radiance that varies with the polarization state of the transmitter beam near the target and just before it is incident on the reference. The transmitter illustratively includes a laser or lamp source inclusive of a polarizer or polarization modulator, and, optionally, a filter that selectively transmits incident radiation. The receiver receives returned radiation from the reference, and determines the radiance of the photoexcited radiation. The receiver includes a detector, such as a camera, and may also include an analyzer and a filter that selectively transmits the returned radiation of interest. The returned radiation may be diffuse reflection, in which case the receiver may require a polarizer to filter out the specular reflection. The returned radiation may be photoluminescence (including fluorescence) or Raman scattering, and optionally has a wavelength significantly different from the incident radiation. In this case, the receiver optionally includes a filter that transmits the photoluminescence while blocking radiation at the incident radiation wavelength. The shape and optical properties of the reference object are known, the distance between source/ receiver system, and reference object are known, but the depolarization strength of the environment between source/receiver system and reference object are unknown and must be calculated.
- The signal processing circuitry, such as a computer, is able to calculate the depolarization efficiency of the environment from the initial polarization, final polarization, and the reference distance. The circuitry receives detector data from the detector, the detector data being correlated with the intensity of returned radiation. The apparatus optionally includes timing electronics, to allow detector data to be correlated with initial polarization, the timing electronics being in electronic communication with the polarization modulator and with the detector. The apparatus may receive the reference distance from an associated device with which the apparatus is in communication.
- The reference is already present within the environment, or may be introduced for inventive purpose. In one example, an asphalt road could be a photoluminescent target that is already in the environment. In a second example, a plastic target of known shape and composition could be introduced in the environment as a target for atmospheric studies. In either case, the shape and orientation of the target is what makes the intensity of emission dependent on transmitter polarization state. The depolarization strength of the medium can be determined using the object distance, the initial polarization of the incident radiation, the orientation and shape of the object, and the variation of the radiance of the received radiation with the polarization of the transmitted radiation.
-
FIG. 1 shows a schematic of an apparatus used to determine depolarization efficiency of an environment using photoluminescence measurements and temporal variations in receiver intensity. -
FIG. 2 shows a schematic of an apparatus used to determine depolarization efficiency of an environment using photoluminescence measurements and spatial variations in receiver intensity. - The present invention has utility as part of a remote imaging method, enabling the remote imaging method to compensate for image distortion associated with light passing through a distorting environment. A method is provided according to the present invention for measuring depolarization efficiency using a polarimetric stereo approach. A volumetric (i.e., three-dimensional) object with known properties is acquired as a reference object (or reference), illuminated with an electromagnetic radiation source that is completely polarized and at a known distance from the reference, and imaged using either photoluminescence or diffuse reflectance from the reference. The variations in radiance intensity at the receiver is measured and correlated to the polarization of the electromagnetic source. From the variations of emission radiance at the receiver, the degree of depolarization between the electromagnetic source and the receiver can be calculated using the correlation. The depolarization efficiency is the fraction of polarized radiation depolarized in a unit distance.
- An inventive method also is provided that uses the variations in pixel intensity to calculate the depolarization without any temporal variation of laser polarization. The detector is a camera, where the intensity of received radiation is measured at each pixel. The polarization-averaged intensity at each pixel is proportionate to the depolarization of the object at each corresponding object point, where the transmittance at each object point is a function of both the polarization state of the incident radiation at the object surface and the angle of incidence at that object point. The three-dimensional shape of the target is known, so the depolarization of the surface of the reference-object can be calculated at each point. The variations in radiance intensity at each pixel of the receiver is measured and correlated to the polarization of the electromagnetic source. The depolarization can be calculated by dividing the measured polarization of the return radiation by the depolarization of the surface. The distance-averaged depolarization efficiency of the atmosphere between the object and the radiation source is found from the difference in degree of polarization between the radiation source and the object surface, divided by the distance to the reference.
- The geometric shape of the reference can be chosen to present a curved surface, such as a circular cross-section, to the incident light source. For example, the reference can be a cylinder. For a cylindrical reference, the radiance values along the axis vary less with polarization than radiance values around the circumference. The composition, at least of a surface layer of the reference, can be chosen to present desired optical properties, such as photoluminescence or diffuse reflection, as described in more detail below.
- An exemplary inventive apparatus for determining the depolarization efficiency of an environment includes a transmitter, inclusive of a radiation source that provides the incident radiation, and a receiver, inclusive of a radiation detector. The radiation detector is optionally a laser, and the transmitter optionally includes a polarizer (a fixed polarizer or polarization modulator), and the transmitter optionally includes a camera and an analyzer. An exemplary analyzer includes a fixed polarizer with a polarization transmission detection orthogonal to that of a fixed polarizer in the transmitter.
- Apparatus and methods according to the present invention are readily used to determine the depolarization efficiency of an environment in which the reference is located. The incident radiation passes through the environment on the way to the object. Determination of environmental depolarization efficiency is critical to improving remote object imaging and tracking through the environment and as such has applications in satellite, atmospheric, and oceanographic imaging. In the case where the environment is the atmosphere, the incident radiation is depolarized by atmospheric particles. Therefore, if the shape of the reference is known independently of the photoluminescent image, then the depolarization efficiency of the atmosphere between a polarized radiation source and the reference is calculated. The dependence of specular reflectivity on incident angle and polarization of the incident radiation is used to determine stereo information from radiation returned from the reference (such as the photoluminescence emission intensity, or diffuse reflectance). Apparatus according to embodiments of the present invention is also used to determine three-dimensional shape information of remote objects, and spectroscopic information relating to an object surface may also be also obtained.
- In embodiments of the present invention, a source of electromagnetic radiation with time-varying differential-polarization provides incident radiation that illuminates a remote object. Photoexcited emission (i.e., photoluminescence, Raman scattering, or diffuse reflection) is excited in the remote object, and time variations in photoluminescence intensity (or emission intensity) are used to form an image of the object. The time variations in photoluminescence intensity allow stereo vision information (i.e. three-dimensional information) to be determined for the object, and can be used to calculate a surface-height profile of the object from a single viewpoint. Photoluminescence spectra, regardless of whether the spectra are excitation and emission based, contain chemical information on the composition of points on the object. The difference between emission intensities at two polarization states, divided by the time-averaged emission intensity, is sensitive to the three-dimensional contours of the object. Therefore, the radiance of emission measured at the receiver is sensitive both to the three-dimensional contours of the object and to the orientation of the object.
- The difference between photoluminescence intensities at the two incident radiation polarizations varies between one angle of incidence and another, hence varies at different incident points on a curved reference surface. The polarimetric photoluminescence signal is a function of time variations in emission intensity. The difference in photoluminescence intensities at two polarization states divided by the polarization-averaged photoluminescence intensity is designated the degree of polarization of the polarimetric photoluminescence signal. The degree of polarization of the polarimetric photoluminescence signal differs from the degree of polarization of emitted photoluminescence, which describes the polarization of emitted radiation.
- The specular reflectance of the object varies with the polarization of the incident radiation, because incident radiation that is removed by specular reflection is not available to become photoluminescence emission, as described in Miyazaki et al., “Determining surface orientations of transparent objects based on polarization degrees in visible and infrared wavelengths,” J. Opt. Soc. Am. A 19, 687-694 (2004). Hence, the emission intensity is anti-correlated with the specular reflectance due to radiative transfer, related to the conservation of energy. Therefore, if the incident angle is greater than zero but less than a critical angle, and if the total intensity of incident radiation is constant in time, this intensity of emission will vary with the polarization of the incident radiation.
- The photoluminescence carries information on the three-dimensional shape of the object, because specular reflectance varies with incident angle as well as incident polarization. Surface curvature and changes in angle of incidence are thus embedded in the time variation of emission intensity. However, if the three-dimensional structure of the target surface and the distance to the target is determined a priori on a reference object, the response of the photoluminescence to incident radiation can be used to determine the strength of depolarization of the medium (i.e., atmosphere or water). Therefore, if the object has a curved or bent surface, the photoluminescence across the surface varies with position. This invention concerns the use of polarization measurements in photoexcited emission or diffuse reflection to determine depolarization of the medium.
- In the case of a dielectric reference, the emission intensity varies with the linear polarization of the incident radiation. The Fresnel equations govern the specular reflectance of dielectric materials and indicate that the reflectance of s-polarized radiation is greater than reflectance of p-polarized radiation.
-
FIG. 1 shows a schematic of atmospheric depolarization efficiency measured by using a reference having photoluminescence, and incident radiation having time-varying degree of initial polarization. Electromagnetic radiation from aradiation source 10 passes through apolarization modulator 12 andexcitation wavelength filter 14. The resulting incident radiation then passes through an environment, in this example the atmosphere, and is incident on the outer surface of a reference object (or more concisely, a reference) 16.FIG. 1 shows the incident radiation, I, incident at anincidence point 18 on thereference 16, and also shows a dashed line representing the tangent to the surface at the incidence point. A specular reflection beam is shown as R, and a transmitted beam (T) is transmitted into the reference and may be completely absorbed. Photoluminescence emission P, induced by the incident radiation, is collected by alens 20, passes throughemission wavelength filter 22, and is incident on adetector 24, in this example a camera. Thewavelength filter 22 operates to separate the photoluminescence from reflected light. -
Signal processing circuitry 26 is in communication with thepolarization modulator 12 anddetector 24, so that detected photoluminescence intensity is correlated with incident polarization. Thedetector 24 is illustratively a camera producing an intensity image matrix for photoluminescence. Three-dimensional data relating to the object may be shown ondisplay 28. - The incident radiation I has time-varying differential polarization, Po(t), where Po is an initial degree of polarization (before passing through the environment) and t is time, and constant polarization-averaged intensity. The incident radiation passes through the environment, in this example the atmosphere, where it is partially depolarized by atmospheric particles. The radiation incident on the surface of the reference has a degree of polarization P(t+y/c), where P(t) is degree of polarization on the reference surface, t is time that the laser pulse is emitted and y is the distance between laser and surface.
- The reference is preferably an object having a known shape, such as a cylinder or sphere, or other shape having a known cross-section or a shape that may be approximated. The reference has a surface that produces photoluminescence on excitation by the incident radiation. For example, the reference may have an outer layer, such as a coating containing a luminescent substance. A laser radiation source operating at a wavelength that excites photoluminescence from the surface reference is well suited for this exemplary inventive methodology.
- Photoluminescence emission is generated that has a radiance that varies with time and angle of incidence. The depth of modulation (i.e., emission intensity for one polarization state of excitation minus the emission state for the orthogonal state of polarization) is recorded by the system and depends on the angle of incidence, and so contains information on the degree of polarization, P(t).
- The time electronics stores the initial polarization P0(t) and the fractional change in emission intensity P(t), and from this the total depolarization factor P/P0 is calculated. From the distance, y, over which the depolarization occurs, the path-averaged depolarization efficiency can be calculated. The path-averaged depolarization efficiency is here defined as β=[−ln(P/P0)]/y.
-
FIG. 2 shows the reference is a known three-dimensional shape, such as a cylinder or sphere, having an outer surface comprising a material producing both diffuse and specular reflectance at the wavelength, λ of the incident radiation. The electromagnetic radiation source in this example is a laser having a laser emission wavelength and in this example the radiance of photoexcited emission or diffuse reflected radiation is measured using a laser beam having a constant (time-independent) intensity and polarization. In the case of photoexcited emission (photoluminescence or Raman), no polarizer is necessary on the camera receiver end because the photoexcited radiation is at a different wavelength than the transmitter radiation (i.e., λi≠λe). However, in the case of diffuse reflected radiation, a polarizer on the receiver end is necessary to eliminate the specular reflected radiation because the photoexcited radiation is at the same wavelength as the transmitter information (i.e., λi=λe). - The narrow band filters on source and camera both pass the wavelength and absorb other wavelengths. The path-averaged depolarization efficiency can be calculated using the distance, y, over which depolarization occurs in the environment, in this case between the radiation source and the reference. In this example, the laser beam is fully polarized in the vertical direction. Diffuse reflection (scattered radiation) from the reference is imaged and filtered by the
analyzer 54 set orthogonal to the polarization of the laser. In this example the analyzer has a horizontal orientation (out of the plane of the paper) so that the specular reflection is blocked. In this context, an analyzer is a polarizer within the receiver. From the variation of intensity of the diffuse reflectance on the image, the polarization state of the incident radiation can be calculated. Using the polarization state on the object, and the distance y between object and source, the depolarization efficiency β can be calculated. - A technique using the following mathematical procedure can be applied to the examples shown in
FIG. 1 andFIG. 2 . The procedure is based on a novel polarimetric equation which describes the photoexcited emission, at a specific emission wavelength, from an ideal surface: -
ρ=cos2(θ−arccos(m −1 sin θ))cos2(φ−ψ)+sin2(φ−ψ) - where ρ is the depolarizance of the surface of the reference object, m is the index of refraction of the object, ψ is an angle of transmitter polarization, θ is the angle of incidence, and φ is the azimuthal angle. The method is based on the idea of measuring the luminescence at least two different times. One time would be when φ−ψ=0°, and the other time when φ−ψ=90°. Based on this equation, the following method can be shown for the determination of D. The depolarization efficiency, D, can be determined by the following equation,
-
P=P 0 exp(−Dy) -
where, -
P 0=2(1−ρ0)/(1+ρ0), -
ρ0=cos2(θ−arccos(m −1 sin θ)), -
and -
P=L 2 /L 1, - where L1 is the luminescence intensity measured when φ−ψ=0° and L2 is the luminescence intensity measured when φ−ψ=90°. However, P is calculated differently for the two cases in
FIG. 1 andFIG. 2 . The measured luminescence in both cases is described by a function L(ψ;φ,θ,y) where L is the luminescence intensity. In the case ofFIG. 1 , ψ varies with time, t. The intensity L is measured at various times, t. The other angles (φ and θ) are fixed during the determination. The polar angle, θ, must be known previous to the determination. The polarization for this case is, -
P=L(φ;φ+90°,θ,y)/L(φ;φ,θ,y), - The angle φ may be known before the determination of D. However, a variation on the technique would be that the angle φ be determined from the values measured of L at various times, t. In the case of
FIG. 2 , φ varies with the position on the object. The other angles (ψ and θ) are fixed during the determination. The angles, ψ and θ, must be known and fixed to the determination. The polarization for this case is, -
P=L(ψ;ψ+90°,θ,y)/L(ψ;ψ,θ,y). - The above method will work for photoluminescence emission, Raman scattering or diffuse reflection. D can be calculated in the case of
FIG. 1 if the polarization varies with time. D can be calculated in the case ofFIG. 2 when the polarization is constant in time. - The total polarization of the received light at distance z, which includes the depolarization of the atmosphere, is Pf where
-
R f=ρa/ρ. - The strength of depolarization, D, of the medium between transmitter and receiver is calculated using the following formula.
-
R f=exp(−Dy). - The reference object, also referred to more concisely as a reference, may be a natural or man-made object. For example, a reference may be positioned a certain distance from the apparatus by placement, firing a projectile, dropping from an aircraft, or other method, at the time of measurement or at some earlier time. The reference may include an electronic circuit or transponder, so as to facilitate determination of the reference object distance. In other examples, a locator device may be placed on a natural object. The reference can be an object chosen to have desired properties, such as photoluminescence or diffuse scattering. The reference can be an object presenting a curved surface, for example providing a plurality of incident angles for incident radiation.
- The reference distance is the path length of the incident radiation through the environment to the point of incidence on the reference. For atmospheric depolarization determination, the reference distance may be much larger than the dimensions of the apparatus used, and may also be much larger than the size of the reference object. Hence, the reference distance may be equivalently referred to as, for example, the distance between the radiation source and the center of the reference object, without any significant loss of accuracy. For other media, or with shorter path lengths, determining the position of the point of incidence on the reference object is used in order to confirm the path length.
- The reference distance may be determined by satellite imaging, a ranging device (such as laser ranging device, ultrasound ranging device, optical rangefinder, and the like), relative absolute position as determined using a GPS or similar device, from the angle subtended at the apparatus location (for an object of known size), apparent object position relative to known landmarks, or any other distance measurement method. The apparatus may include additional functionalities related to reference distance determination, such as ranging devices, wireless transceiver in communication with the reference (if appropriate), and the like. For example, the radiation source is optionally a pulsed laser, so that ranging can be achieved using gating times.
- The reference distance can be determined using photometry, such as photometric stereo measurements using a color camera.
- The transmitter provides incident radiation having desired properties. The transmitter comprises at least one radiation source, such as a laser, and optionally includes additional components to modify the properties of the incident beam, such as one or more lenses, an aperture, a polarizer or polarization modulator, a filter, and the like. The transmitter also optionally includes beam steering components to select an incidence location on a reference. Other possible transmitters include lamps and light emitting diodes.
- The incident wavelength may be varied, for example by selecting one of a plurality of radiation sources, tuning a radiation source (such as a dye laser or wavelength adjustable semiconductor laser), or modifying or changing a filter.
- The radiation source may be a directional source of electromagnetic radiation such as a laser providing laser radiation. The laser radiation is inherently polarized, or alternatively is polarized by an external polarizer. The laser radiation polarization is controllable by electrical signals applied to the laser, for example applied to an electrooptic material within the laser structure. Electrical signals are applied to a laser, such as a semiconductor laser structure, to control polarization and/or emission wavelength.
- A polarization modulator, if used, may be an electrooptic modulator, for example comprising an electrooptic crystal. In other examples, the function of a polarization modulator is included in a laser. For example, a laser, such as a semiconductor laser, is optionally used having a laser radiation output having electrically controllable polarization properties.
- The receiver comprises the detector and any associated components, such as a lens or filter, are used to collect and detect the returned radiation.
- Returned radiation from the reference, such as photoluminescence or diffuse reflection, is collected by the receiver. The receiver includes a detector, such as an optical detector. The receiver is sensitive to at least one spectrum of: visible wavelengths, IR wavelengths (including near-IR and thermal radiation wavelengths), THz, GHz, or other electromagnetic radiation ranges. For example, the receiver is sensitive to near-IR wavelengths and visible, or UV-visible ranges. For diffuse reflected radiation, a polarizer at the receiver end is used to eliminate specular reflected radiation from the receiver.
- It is appreciated that the returned radiation also is optionally Raman scattering. Holographic filters in the receiver are used to selectively transmit Raman scattered radiation.
- Apparatus according to the present invention preferably includes signal processing circuitry, operational to determine the depolarization of the environment from the initial polarization, the final polarization, and the reference distance. The signal processing circuitry allows the detector signal to be correlated with the polarization modulator, if used, or equivalently with the polarization output of the radiation source if this is controllable. The signal processing circuitry operates on a computer, the computer including a processor, clock, memory, data input, and data output (such as a display driver coupled to a display or transmitter in communication with a network). The processor may execute one or more algorithms to provide the results of calculations described herein.
- In examples discussed above, the environment discussed is the atmosphere. However, examples of the present invention can be used to determine depolarization of various media, such as other gases, liquids (such as water, including river water and sea water), and solids, such as glasses.
- The transmitter and receiver may be disposed within a single housing, to form a unitary apparatus. The apparatus is carried or otherwise supported by a land vehicle, boat, submarine, aircraft, satellite, a person, or may be set on the ground, for example using a stand.
- The apparatus optionally includes a display, which may be used to provide a visual indication of determined data. The display also is useful to show the part of the environment towards which the incident radiation is directed, for example in combination with zoom or image enhancement capabilities, in order to direct the incident radiation onto the surface of the desired reference. A camera optionally is used to image the reference object, the same camera being used as a sensor in the receiver, and/or for photometric stereo measurements.
- In other inventive embodiments, the transmitter and receiver are contained in separate housings, and are in data communication through a cable or wireless communications link. It is appreciated that multiple transmitters and/or receivers optionally are used, for example to allow data to be collected for multiple angular positions, wavelengths, reference distances, and the like.
- An apparatus according to an embodiment of the present invention optionally provides for collection of other ambient condition data, such as temperature, pressure, humidity, other meteorological conditions such as cloud cover or precipitation, time, sun position, pollutant analysis, particulate form and concentration, altitude, position (for example, using a GPS), and the like. Atmospheric depolarization may be correlated with ambient conditions, and may be used to interpret data collected under similar conditions.
- An apparatus also optionally includes an operational mode selector, the operational mode selector having a first setting in which photoluminescence is detected, and a second setting in which diffuse reflection is detected.
- An apparatus also optionally includes a data input, which can be the data input of the computer, the data input receiving the object distance so as to allow determination of the depolarization efficiency.
- Ambient condition data is collected by instrumentation included within the device, received directly from other devices over a communications link, or may be entered by an apparatus operator using a data entry mechanism.
- The apparatus also optionally includes a housing, which in some examples may seal against ingress of the environment under test (for example, if the environment is water, such as seawater). The housing may be provided with one or more windows to allow radiation to enter and/or leave the housing.
- As used herein the terminology “photoluminescence” or “photoluminescent radition” refers to a process in which a substance absorbs photons (electromagnetic radiation) and then re-radiates photons. Quantum mechanically, the process can be described as an excitation to a higher energy state and then a return to a lower energy state accompanied by the emission of a photon. The photoluminescent processes include, but are not limited to, resonant radiations, in which a photon of a particular wavelength is absorbed and an equivalent photon is immediately emitted.
- In general, the present invention is not restricted to the illustrative examples described above. Examples are not intended as limitations on the scope of the invention. Methods, apparatus, compositions, and the like described herein are exemplary and not intended as limitations on the scope of the invention. Changes therein and other uses will occur to those skilled in the art. The scope of the invention is defined by the scope of the claims. Patents, patent applications, or publications mentioned in this specification are incorporated herein by reference to the same extent as if each individual document was specifically and individually indicated to be incorporated by reference. Having described the invention, the invention is now claimed.
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