US20100219115A1 - System and method for assembling a large scale chromatography structure - Google Patents
System and method for assembling a large scale chromatography structure Download PDFInfo
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- US20100219115A1 US20100219115A1 US12/663,750 US66375008A US2010219115A1 US 20100219115 A1 US20100219115 A1 US 20100219115A1 US 66375008 A US66375008 A US 66375008A US 2010219115 A1 US2010219115 A1 US 2010219115A1
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- Prior art keywords
- housing
- cartridge
- chromatography
- container system
- mobile phase
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/22—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/14—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the introduction of the feed to the apparatus
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6091—Cartridges
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T29/00—Metal working
- Y10T29/49—Method of mechanical manufacture
- Y10T29/49826—Assembling or joining
Definitions
- the present invention relates to a system and method for assembling a large-scale chromatography structure.
- purification by liquid chromatography is a process of separation of one or more liquid components from all other liquid components of a mixture.
- Large-scale liquid chromatography is currently the biotech industry's most widely utilized, if not the only, means of purification of biopharmaceuticals. Such purification is necessary in order to separate the therapeutic component of interest from all other impurities with which it coexists during the process of manufacture.
- chromatography is carried out in cylindrical vessels, termed columns, which are packed with a chromatography medium itself comprising of porous microscopic spherical particles.
- the liquid mixture containing the therapeutic product of interest is pumped through the column where the therapeutic binds to and is retained by the chromatography medium while the undesirable impurities flow through and out of the column without binding.
- the therapeutic product is thus separated out of the mixture, i.e. it has been purified.
- the therapeutic product can later be unbound from the chromatography medium and extracted, or eluted, from the column.
- a large-scale purification process utilizes 3 or 4 different columns packed with different chromatography media in order to obtain the required high degree of purity.
- HETP Height Equivalent to a Theoretical Plate
- Asymmetry HETP is, in essence, a measure for the efficiency with which the column can perform the separation.
- the asymmetry is in a way a measure of the uniformity of the packed medium, which in itself determines how evenly the process liquid will flow though the column.
- the present invention has been accomplished in view of the above-mentioned technical background, and it is an object of the present invention to provide a system and method for assembling a large-scale chromatography structure.
- a container system in a preferred embodiment of the invention, where the container system includes a housing.
- a chromatography cartridge is disposed vertically in the housing.
- a structural support in the housing is configured to retain the chromatography cartridge within the housing.
- the housing is configured to withstand a certain amount of pressure generated inside the chromatography cartridge.
- another container system in another preferred embodiment of the invention includes a housing.
- a chromatography cartridge is disposed horizontally in the housing.
- a structural support in the housing is configured to retain the chromatography cartridge within the housing.
- the housing is configured to withstand a certain amount of pressure generated inside the chromatography cartridge.
- a method for assembling a chromatography structure includes: providing a housing, wherein the housing is configured to open, wherein the housing is configured to withstand a certain amount of pressure; inserting a cartridge into the housing; and securing the cartridge in the housing.
- FIG. 1 illustrates a block diagram of a large scale chromatography cartridge in accordance with the invention
- FIG. 2 illustrates a block diagram of a housing for the large-scale chromatography cartridge of FIG. 1 in accordance with the invention
- FIG. 3 illustrates a block diagram of another housing for the large-scale chromatography cartridge of FIG. 1 in accordance with the invention.
- FIG. 4 is a flow-chart that of how the large-scale chromatography cartridge of FIG. 1 is inserted into the housing in accordance with the invention.
- FIG. 1 illustrates a block diagram of a chromatography cartridge 100 .
- the chromatography cartridge 100 includes a left tube wall 101 a, a right tube wall 101 b, a top distributor 102 a, a bottom distributor 102 b, a top bed support 103 a, a bottom bed support 103 a, an inlet port 104 and a packing device 105 .
- the inlet port 104 may also be a mobile phase inlet port.
- Packing device 105 may be a packing device nozzle.
- Cartridge 100 is a self-contained device or pre-packed disposable cartridge that is packed with typical chromatography media under controlled conditions, tested and certified it constitutes a ready-to-process disposable chromatography column. When in operation, this cartridge is contained in a suitable mechanical framework or housing that conforms to international pressure-vessel codes and standards. This cartridge is intended for industrial applications in the biopharmaceutical, food and beverage, and other industries where chromatography is utilized in conventional columns. The cartridge is manufactured in sizes representative of industry standards and ranging in diameter from 400 to 2000 MM. The cartridge 100 is placed in the housing 200 as shown in FIG. 2 , and is positioned in a way which provides for a flow direction through the packed bed of chromatography media from top to bottom or bottom to top.
- cartridge 100 When positioned in such a way, cartridge 100 is said to be in a horizontal position.
- the cartridge 100 is placed in the housing 300 as shown in FIG. 3 and is positioned in a way, which provides for a flow direction through the packed bed of chromatography media from side to side, i.e. from left to right or from right to left.
- cartridge 100 When positioned in such a way, cartridge 100 is said to be in a vertical position.
- the vertical position of the cartridge 100 is a desired position where the floor area is limited and lifting devices are unavailable as the cartridge 100 is rolled into position.
- Packing device 105 is a typical packing device or packing nozzle that sits on top of the cartridge 100 .
- This packing device 105 is a removable element that gives access to the interior of the cartridge 100 for the introduction of and packing of typical chromatography media between the top bed support 103 a and bottom bed support 103 b .
- the distributors 102 a and 102 b are utilized to provide equal flow over the entire cross section of the packed cartridge 100 .
- Bottom bed support 103 a and the top bed support 103 b are sealed to the bottom distributor 102 a and top distributor 102 b.
- the purpose of the bottom bed support 103 a and the top bed support 103 b is to contain the chromatography medium within the cartridge 100 but allows the flow of liquid through.
- the bed supports 103 a and 103 b are made from woven material with controlled pore size made from either plastic or metal.
- the typical distributors 102 a and 102 b are fixed and sealed to the standard tube walls 101 a and 101 b.
- the tube walls 101 a 101 bs are utilized to contain the packed chromatography media in the chromatography cartridge 100 and have the appropriate strength to contain the packed chromatography media.
- the tube walls 101 a and 101 b may have a cylindrical, rectangular or any kind of shape and are made from acrylic, polypropylene, polyethylene, other suitable class VI plastics, metal, or other suitable materials accepted by the industry.
- Distributors 102 a and 102 b could be all or in part included within the cartridge 100 insert or as stand alone piece as can the bed supports 103 a and 103 b
- the packing device 105 can be removed and replaced with a mobile phase port similar to the inlet port 104 .
- the inlet port 104 is utilized to provide a pathway for pumping liquids into or out of the /cartridge via the bottom distributor 102 b and the bed support 103 b.
- FIG. 2 shows a housing with the large scale chromatography cartridge of FIG. 1 .
- Housing 200 includes a hinge 201 , a lid 202 , a first mobile phase port 203 , a second mobile phase port 205 , the large-scale chromatography cartridge 100 , rib 207 a, rib 207 b , rib 207 c, rib 207 d, rib 207 e, rib 207 f, rib 207 g, rib 209 a, rib 209 b, rib 209 c, rib 209 d, rib 209 e, rib 209 f, rib 209 g, left leg 211 a and right leg 211 b .
- Hinge 201 is utilized to open housing 200 to insert the chromatography cartridge 100 into the housing 200 and the hinge 201 is utilized to release the chromatography cartridge 100 from the housing 200 .
- Hinge 201 is utilized to open and close the lid 202 that retains the chromatography cartridge 100 in housing 200 .
- the first mobile phase port 203 is utilized to receive the pumped liquids into the chromatography cartridge 100 and the second mobile phase port 205 is for releasing the pumped liquids from the cartridge 100 .
- a nozzle or packing device may be placed on top of the housing 200 to transfer chromatography media into the cartridge 100 .
- the first mobile phase port 203 or the second mobile phase port 205 may be attached to a disposable expansion device (not shown), which allows for any liquid expansion during transit of the cartridge 100 .
- Ribs 207 a - f and ribs 209 a - g are utilized to provide structural support for the cartridge 100 to retain the cartridge 100 in the housing 200 .
- Legs 211 a and 211 b are utilized as support to carry or stabilize the chromatography cartridge 100 .
- the housing 200 is constructed of a metal such as stainless steel or another suitable material or combination of suitable materials. This housing will conform to internationally accepted pressure level codes and standards and will be capable of containing the forces generated within the cartridge 100 during operation of the cartridge 100 . Housing 200 is capable of withstanding a pressure of less than 3 bar (45 psi). As shown in FIG. 2 , the chromatography cartridge 100 is positioned inside the housing where the process liquids flow from the first mobile phase port 203 to the second mobile phase port 205 . In another embodiment of the invention, the process liquids may flow from the second mobile phase port 205 to the first mobile phase port 203 . Cartridge 100 is disposable whereas the housing 200 is not.
- the lid of the housing 200 is opened.
- the cartridge 100 is then removed and a new cartridge is inserted into the housing 200 , then the lid is closed and the system is ready for operation. Since the housing 200 does not come into contact with the product (I.E. the biopharmaceutical or drug) it does not need to be made from materials which must meet relevant FDA regulations.
- FIG. 3 shows another housing with the large scale chromatography cartridge of FIG. 1 .
- Housing 300 includes a central flange 301 , a first mobile phase port 303 , a second mobile phase port 305 , the large-scale chromatography cartridge 100 , rib 307 a, rib 307 b, rib 307 c, rib 307 d, rib 307 e, rib 307 f, rib 307 g, rib 309 a, rib 309 b, rib 309 c, rib 309 d, rib 309 e, rib 309 f, rib 309 g, left rail 311 , right rail 313 , left leg 315 and right leg 317 .
- Housing 300 is equivalent to housing 200 so a description of housing 300 will not be described herein.
- Central flange 301 is utilized to open housing 300 to insert the chromatography cartridge 100 into the housing 300 and the central flange 301 is utilized to release the chromatography cartridge from the housing 300 .
- the central flange 301 may be a bolt or clamp used to retain the cartridge 100 in housing 300 .
- first mobile phase port 303 and second mobile phase port 305 are equivalent to their counterparts first mobile phase port 203 and the second mobile phase port 205 described above so a description will not be included herein.
- Ribs 307 a, 307 b , 307 c, 307 d, 307 e, 307 f and 307 g are equivalent to ribs 207 a, 207 b, 207 c, 207 d, 207 e , 207 f and 207 g described above so a description will not be included herein.
- Ribs 309 a , 309 b, 309 c, 309 d, 309 e, 309 f, 309 g are equivalent to ribs 209 a, 209 b, 208 c, 209 d, 209 e , 209 f and 209 g described above so the description will not be included herein.
- Rail 311 and rail 313 are utilized to allow the two sides of the housing, the left side and the right side, to slide away from each other and thus provide an opening through which cartridge 100 can be inserted and removed from the housing 300 .
- Legs 315 and 317 operate in the same manner as left leg 211 a and right leg 211 b to support the cartridge 100 in the respective housing 300 and housing 200 .
- the chromatography cartridge 100 is positioned in a vertical state. This positioning of the chromatography cartridge 100 enables the process liquids to flow from the first mobile phase port 303 to the second mobile phase port 305 in a left to right motion instead of a top down motion as the cartridge 100 in FIG. 2 .
- the process liquids may flow in a right to left motion in the cartridge 100 in housing 300 .
- Housing 300 is constructed from the same materials as housing 200 .
- housing 300 occupies significantly less floor space; and does not require use of hoists or other lifting devices for removing and inserting cartridges.
- the center flange 301 is unbolted or unclamped, and then the two halves of the housing 300 slide open along the rails 311 and 313 .
- the old cartridge 100 is then rolled out and the new cartridge is rolled in.
- FIG. 4 shows a flow-chart of how the cartridge is inserted into the housing.
- a user opens the housing 200 ( FIG. 2 ). Housing 200 is opened when the user opens the lid 201 .
- the user opens up the housing 300 ( FIG. 3 ) by unbolting or unclamping the center flange 301 and sliding the two halves along rails 311 and 313 .
- the user at block 403 can insert the chromatography cartridge 100 into the housing 200 .
- Cartridge 100 typically includes a storage solution that is used for the prevention of bacterial growth in the cartridge when the cartridge 100 is in transit to the user.
- the storage solution may be 20% ethanol, an antimicrobial agent, weak benzyl alcohol solution or a weak caustic solution.
- the cartridge 100 is secured in the housing 200 by the multiple ribs 209 a - g and 207 a - g and then the housing is closed when the lid 201 is closed.
- the housing 300 FIG. 3
- the cartridge 100 is secured in the housing 300 by the multiple ribs 309 a - g and 307 a - g and then the housing 300 is closed when the center flange 301 is bolted or clamped.
- the user connects the chromatography structure as shown FIG. 2 or FIG. 3 . to a liquid processing unit.
- the standard liquid processing unit includes: a skid, sanitary hoses, sanitary clamps and gaskets.
- Mobile phase ports 203 and 205 are connected to the standard skid.
- Housing 200 and cartridge 100 are designed in such a way that when the lid 201 is closed the mobile phase ports 203 and 205 will be accessible so the necessary connections to the liquid processing unit, i.e. the skid, can be easily made.
- the user washes a storage solution (20% ethanol) out of the cartridge 100 by utilizing a water or buffer that is transferred through the liquid processing unit and mobile phase port 203 through the cartridge 100 out of the mobile phase port 205 .
- the user washes the storage solution out of the cartridge 100 by utilizing a water or buffer that is transferred through the mobile phase port 303 through the cartridge 100 and out of the mobile phase port 305 .
- the user may test the washed cartridge to verify test results provided by the manufacturer of the cartridge in the certificate of analysis.
- the cartridge 100 is ready for use by the user.
- the user determines if the cartridge 100 can be used again. If it is determined that it can be used again then the process returns to block 407 , if the cartridge 100 can not be used again then at block 411 the cartridge 100 is removed from the housing 200 . After the user removes cartridge 100 by taking the cartridge 100 out of housing 200 by opening the lid 201 and removing the cartridge 100 , then this process ends. In another embodiment of the invention, when the user removes the cartridge 100 from the housing 300 by unbolting or unclamping the central flange and removing the cartridge 100 , then the process ends. The user may insert a new cartridge in the housing 200 . In another embodiment of the invention, the user may insert a new cartridge in the housing 300 .
- This invention provides a system and method for assembling a large-scale chromatography structure.
- This large-scale chromatography structure provides the user with an immediately available and fully functioning cartridge that the user can use without worry about the complexity of packing it and using it. Thus, the user can avoid repeated packing and unpacking that cause unnecessary delays in manufacturing valuable therapeutic products.
- this large-scale chromatography structure is able to withstand pressures that may be exerted on the cartridge as the cartridge undergoes testing.
- this invention provides the user with a simple product that can eliminate all failures and uncertainties of on-site column packing and testing.
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Abstract
A container system is disclosed, where the container system includes a housing. A chromatography cartridge is disposed in the housing. A structural support in the housing is configured to retain the chromatography cartridge within the housing. The housing is configured to withstand a certain amount of pressure generated inside the chromatography cartridge. The chromatography cartridge is disposed in the housing either in horizontal or in vertical position.
Description
- This application is a filing under 35 U.S.C. §371 and claims priority to international patent application number PCT/US2008/067338 filed Jun. 18, 2008, published on Dec. 31, 2008, as WO 2009/002780, which claims priority to U.S. provisional patent application No. 60/946,404 filed Jun. 27, 2007; the disclosure of which is incorporated herein by reference in its entirety.
- The present invention relates to a system and method for assembling a large-scale chromatography structure.
- Generally, purification by liquid chromatography is a process of separation of one or more liquid components from all other liquid components of a mixture. Large-scale liquid chromatography is currently the biotech industry's most widely utilized, if not the only, means of purification of biopharmaceuticals. Such purification is necessary in order to separate the therapeutic component of interest from all other impurities with which it coexists during the process of manufacture.
- Large-scale chromatography is carried out in cylindrical vessels, termed columns, which are packed with a chromatography medium itself comprising of porous microscopic spherical particles. In a typical purification procedure, the liquid mixture containing the therapeutic product of interest is pumped through the column where the therapeutic binds to and is retained by the chromatography medium while the undesirable impurities flow through and out of the column without binding. The therapeutic product is thus separated out of the mixture, i.e. it has been purified. The therapeutic product can later be unbound from the chromatography medium and extracted, or eluted, from the column. Normally, a large-scale purification process utilizes 3 or 4 different columns packed with different chromatography media in order to obtain the required high degree of purity.
- In the industry, when a packed bed of media contained within a column has been used several times to purify several batches of the therapeutic product, the chromatography medium may become incapable of performing the purification and therefore must be taken out and replaced. The process of removing the spent chromatography medium from the column is called unpacking Similarly, the process of filling the same column with new chromatography medium is called packing Therefore, the column itself can be viewed as a fixed piece of equipment whereas the chromatography medium is viewed as a consumable.
- Currently, the chromatography columns utilized by the industry need to be unpacked and repacked frequently. Such unpacking and repacking is always carried out on site and is performed by operators who are trained and highly skilled in the art. Also, these operators may require specialized training and a high skill level to unpack and repack chromatography columns. The packing of a column with chromatography medium is a critical and crucial process because it is the quality of the packed bed of medium that will determine how well the column will perform the separation. A poorly packed column will not perform the separation task assigned to it. Furthermore, a poorly packed column becomes unstable and its performance is unpredictable and inconsistent—a highly undesirable state of affairs.
- To ensure consistency and reproducibility of column performance, every newly packed column is subjected to an evaluation test, or a battery of tests, whose objective is to determine whether or not the column can perform the separation expected of it. There are two commonly used parameters for measuring a column's performance: 1) HETP (Height Equivalent to a Theoretical Plate) and 2) Asymmetry. HETP is, in essence, a measure for the efficiency with which the column can perform the separation. The asymmetry is in a way a measure of the uniformity of the packed medium, which in itself determines how evenly the process liquid will flow though the column.
- Due to the complexity of packing procedures and the skills required to perform the packing, newly packed columns in the biotech industry frequently fail the evaluation tests. Such columns need to be unpacked, repacked and tested. These three processes are lengthy and costly. More importantly, repeated packing and unpacking cause unnecessary delays in manufacturing of valuable therapeutic products. Ultimately, this results in reduced quantities of product manufactured annually and subsequently to a substantial revenue loss. Therefore, there is a need for a product, which can greatly reduce the uncertainties and failures from on site packing
- The present invention has been accomplished in view of the above-mentioned technical background, and it is an object of the present invention to provide a system and method for assembling a large-scale chromatography structure.
- In a preferred embodiment of the invention a container system is disclosed, where the container system includes a housing. A chromatography cartridge is disposed vertically in the housing. A structural support in the housing is configured to retain the chromatography cartridge within the housing. The housing is configured to withstand a certain amount of pressure generated inside the chromatography cartridge.
- In another preferred embodiment of the invention another container system is disclosed that includes a housing. A chromatography cartridge is disposed horizontally in the housing. A structural support in the housing is configured to retain the chromatography cartridge within the housing. The housing is configured to withstand a certain amount of pressure generated inside the chromatography cartridge.
- In yet another preferred embodiment of the invention, a method for assembling a chromatography structure is disclosed that includes: providing a housing, wherein the housing is configured to open, wherein the housing is configured to withstand a certain amount of pressure; inserting a cartridge into the housing; and securing the cartridge in the housing.
- These and other advantages of the present invention will become more apparent as the following description is read in conjunction with the accompanying drawings, wherein:
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FIG. 1 illustrates a block diagram of a large scale chromatography cartridge in accordance with the invention; -
FIG. 2 illustrates a block diagram of a housing for the large-scale chromatography cartridge ofFIG. 1 in accordance with the invention; -
FIG. 3 illustrates a block diagram of another housing for the large-scale chromatography cartridge ofFIG. 1 in accordance with the invention; and -
FIG. 4 is a flow-chart that of how the large-scale chromatography cartridge ofFIG. 1 is inserted into the housing in accordance with the invention. - The presently preferred embodiments of the invention are described with reference to the drawings, where like components are identified with the same numerals. The descriptions of the preferred embodiments are exemplary and are not intended to limit the scope of the invention.
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FIG. 1 illustrates a block diagram of achromatography cartridge 100. Thechromatography cartridge 100 includes aleft tube wall 101 a, aright tube wall 101 b, atop distributor 102 a, abottom distributor 102 b, atop bed support 103 a, abottom bed support 103 a, aninlet port 104 and apacking device 105. Theinlet port 104 may also be a mobile phase inlet port.Packing device 105 may be a packing device nozzle. - Cartridge 100 is a self-contained device or pre-packed disposable cartridge that is packed with typical chromatography media under controlled conditions, tested and certified it constitutes a ready-to-process disposable chromatography column. When in operation, this cartridge is contained in a suitable mechanical framework or housing that conforms to international pressure-vessel codes and standards. This cartridge is intended for industrial applications in the biopharmaceutical, food and beverage, and other industries where chromatography is utilized in conventional columns. The cartridge is manufactured in sizes representative of industry standards and ranging in diameter from 400 to 2000 MM. The
cartridge 100 is placed in thehousing 200 as shown inFIG. 2 , and is positioned in a way which provides for a flow direction through the packed bed of chromatography media from top to bottom or bottom to top. When positioned in such a way,cartridge 100 is said to be in a horizontal position. Alternatively, thecartridge 100 is placed in thehousing 300 as shown inFIG. 3 and is positioned in a way, which provides for a flow direction through the packed bed of chromatography media from side to side, i.e. from left to right or from right to left. When positioned in such a way,cartridge 100 is said to be in a vertical position. The vertical position of thecartridge 100 is a desired position where the floor area is limited and lifting devices are unavailable as thecartridge 100 is rolled into position. -
Packing device 105 is a typical packing device or packing nozzle that sits on top of thecartridge 100. Thispacking device 105 is a removable element that gives access to the interior of thecartridge 100 for the introduction of and packing of typical chromatography media between thetop bed support 103 a andbottom bed support 103 b. Thedistributors cartridge 100.Bottom bed support 103 a and thetop bed support 103 b are sealed to thebottom distributor 102 a andtop distributor 102 b. The purpose of thebottom bed support 103 a and thetop bed support 103 b is to contain the chromatography medium within thecartridge 100 but allows the flow of liquid through. The bed supports 103 a and 103 b are made from woven material with controlled pore size made from either plastic or metal. - These
typical distributors standard tube walls tube walls 101 a 101 bs are utilized to contain the packed chromatography media in thechromatography cartridge 100 and have the appropriate strength to contain the packed chromatography media. Also, thetube walls Distributors cartridge 100 insert or as stand alone piece as can the bed supports 103 a and 103 b - When the
cartridge 100 is filled with the packed chromatography media, then thepacking device 105 can be removed and replaced with a mobile phase port similar to theinlet port 104. Theinlet port 104 is utilized to provide a pathway for pumping liquids into or out of the /cartridge via thebottom distributor 102 b and thebed support 103 b. -
FIG. 2 shows a housing with the large scale chromatography cartridge ofFIG. 1 .Housing 200 includes ahinge 201, alid 202, a firstmobile phase port 203, a secondmobile phase port 205, the large-scale chromatography cartridge 100,rib 207 a,rib 207 b,rib 207 c,rib 207 d,rib 207 e,rib 207 f,rib 207 g,rib 209 a,rib 209 b,rib 209 c,rib 209 d,rib 209 e,rib 209 f,rib 209 g,left leg 211 a andright leg 211 b.Hinge 201 is utilized to openhousing 200 to insert thechromatography cartridge 100 into thehousing 200 and thehinge 201 is utilized to release thechromatography cartridge 100 from thehousing 200.Hinge 201 is utilized to open and close thelid 202 that retains thechromatography cartridge 100 inhousing 200. The firstmobile phase port 203 is utilized to receive the pumped liquids into thechromatography cartridge 100 and the secondmobile phase port 205 is for releasing the pumped liquids from thecartridge 100. A nozzle or packing device (not shown) may be placed on top of thehousing 200 to transfer chromatography media into thecartridge 100. In another embodiment of the invention after packing thecartridge 100 with chromatography media, the firstmobile phase port 203 or the secondmobile phase port 205 may be attached to a disposable expansion device (not shown), which allows for any liquid expansion during transit of thecartridge 100. Ribs 207 a-f and ribs 209 a-g are utilized to provide structural support for thecartridge 100 to retain thecartridge 100 in thehousing 200.Legs chromatography cartridge 100. - The
housing 200 is constructed of a metal such as stainless steel or another suitable material or combination of suitable materials. This housing will conform to internationally accepted pressure level codes and standards and will be capable of containing the forces generated within thecartridge 100 during operation of thecartridge 100.Housing 200 is capable of withstanding a pressure of less than 3 bar (45 psi). As shown inFIG. 2 , thechromatography cartridge 100 is positioned inside the housing where the process liquids flow from the firstmobile phase port 203 to the secondmobile phase port 205. In another embodiment of the invention, the process liquids may flow from the secondmobile phase port 205 to the firstmobile phase port 203.Cartridge 100 is disposable whereas thehousing 200 is not. When thecartridge 100 expires or has been used for a predetermined number of cycles, the lid of thehousing 200 is opened. Thecartridge 100 is then removed and a new cartridge is inserted into thehousing 200, then the lid is closed and the system is ready for operation. Since thehousing 200 does not come into contact with the product (I.E. the biopharmaceutical or drug) it does not need to be made from materials which must meet relevant FDA regulations. -
FIG. 3 shows another housing with the large scale chromatography cartridge ofFIG. 1 .Housing 300 includes acentral flange 301, a firstmobile phase port 303, a secondmobile phase port 305, the large-scale chromatography cartridge 100,rib 307 a,rib 307 b,rib 307 c,rib 307 d,rib 307 e,rib 307 f,rib 307 g,rib 309 a,rib 309 b,rib 309 c,rib 309 d,rib 309 e,rib 309 f,rib 309 g, leftrail 311,right rail 313,left leg 315 andright leg 317.Housing 300 is equivalent tohousing 200 so a description ofhousing 300 will not be described herein.Central flange 301 is utilized to openhousing 300 to insert thechromatography cartridge 100 into thehousing 300 and thecentral flange 301 is utilized to release the chromatography cartridge from thehousing 300. Thecentral flange 301 may be a bolt or clamp used to retain thecartridge 100 inhousing 300. - The components first
mobile phase port 303 and secondmobile phase port 305 are equivalent to their counterparts firstmobile phase port 203 and the secondmobile phase port 205 described above so a description will not be included herein.Ribs ribs Ribs ribs Rail 311 andrail 313 are utilized to allow the two sides of the housing, the left side and the right side, to slide away from each other and thus provide an opening through whichcartridge 100 can be inserted and removed from thehousing 300.Legs left leg 211 a andright leg 211 b to support thecartridge 100 in therespective housing 300 andhousing 200. - For this
housing 300, thechromatography cartridge 100 is positioned in a vertical state. This positioning of thechromatography cartridge 100 enables the process liquids to flow from the firstmobile phase port 303 to the secondmobile phase port 305 in a left to right motion instead of a top down motion as thecartridge 100 inFIG. 2 . Referring toFIG. 3 , in another embodiment of the invention, the process liquids may flow in a right to left motion in thecartridge 100 inhousing 300.Housing 300 is constructed from the same materials ashousing 200. - The advantages of using
housing 300 are: 1. it occupies significantly less floor space; and does not require use of hoists or other lifting devices for removing and inserting cartridges. When the expired or usedcartridge 100 needs to be removed, thecenter flange 301 is unbolted or unclamped, and then the two halves of thehousing 300 slide open along therails old cartridge 100 is then rolled out and the new cartridge is rolled in. -
FIG. 4 shows a flow-chart of how the cartridge is inserted into the housing. At block 401, a user opens the housing 200 (FIG. 2 ).Housing 200 is opened when the user opens thelid 201. In another embodiment of the invention, the user opens up the housing 300 (FIG. 3 ) by unbolting or unclamping thecenter flange 301 and sliding the two halves alongrails FIG. 4 , when thehousing 200 is open then the user atblock 403 can insert thechromatography cartridge 100 into thehousing 200.Cartridge 100 typically includes a storage solution that is used for the prevention of bacterial growth in the cartridge when thecartridge 100 is in transit to the user. The storage solution may be 20% ethanol, an antimicrobial agent, weak benzyl alcohol solution or a weak caustic solution. Thecartridge 100 is secured in thehousing 200 by the multiple ribs 209 a-g and 207 a-g and then the housing is closed when thelid 201 is closed. In another embodiment of the invention, the housing 300 (FIG. 3 ) is opened, and then the user inserts thechromatography cartridge 100 into thehousing 300. Thecartridge 100 is secured in thehousing 300 by the multiple ribs 309 a-g and 307 a-g and then thehousing 300 is closed when thecenter flange 301 is bolted or clamped. - At block 405, the user connects the chromatography structure as shown
FIG. 2 orFIG. 3 . to a liquid processing unit. The standard liquid processing unit includes: a skid, sanitary hoses, sanitary clamps and gaskets.Mobile phase ports Housing 200 andcartridge 100 are designed in such a way that when thelid 201 is closed themobile phase ports block 407, the user washes a storage solution (20% ethanol) out of thecartridge 100 by utilizing a water or buffer that is transferred through the liquid processing unit andmobile phase port 203 through thecartridge 100 out of themobile phase port 205. In another embodiment of the invention, atblock 407 the user washes the storage solution out of thecartridge 100 by utilizing a water or buffer that is transferred through themobile phase port 303 through thecartridge 100 and out of themobile phase port 305. Optionally, the user may test the washed cartridge to verify test results provided by the manufacturer of the cartridge in the certificate of analysis. Thecartridge 100 is ready for use by the user. - Next, at
block 409 the user determines if thecartridge 100 can be used again. If it is determined that it can be used again then the process returns to block 407, if thecartridge 100 can not be used again then atblock 411 thecartridge 100 is removed from thehousing 200. After the user removescartridge 100 by taking thecartridge 100 out ofhousing 200 by opening thelid 201 and removing thecartridge 100, then this process ends. In another embodiment of the invention, when the user removes thecartridge 100 from thehousing 300 by unbolting or unclamping the central flange and removing thecartridge 100, then the process ends. The user may insert a new cartridge in thehousing 200. In another embodiment of the invention, the user may insert a new cartridge in thehousing 300. - This invention provides a system and method for assembling a large-scale chromatography structure. This large-scale chromatography structure provides the user with an immediately available and fully functioning cartridge that the user can use without worry about the complexity of packing it and using it. Thus, the user can avoid repeated packing and unpacking that cause unnecessary delays in manufacturing valuable therapeutic products. Also, this large-scale chromatography structure is able to withstand pressures that may be exerted on the cartridge as the cartridge undergoes testing. Thus, this invention provides the user with a simple product that can eliminate all failures and uncertainties of on-site column packing and testing.
- Although the present invention has been described above in terms of specific embodiments, many modification and variations of this invention can be made as will be obvious to those skilled in the art, without departing from its spirit and scope as set forth in the following claims.
Claims (23)
1. A container system comprising:
a housing:
a chromatography cartridge disposed in the housing;
a structural support in the housing configured to retain the chromatography cartridge within the housing; and
the housing is configured to withstand a certain amount of pressure generated inside the chromatography cartridge.
2. The container system of claim 1 , wherein the structural support is a plurality of ribs located in the housing configured to retain the chromatography cartridge.
3. The container system of claim 1 , wherein the housing is made of a metal.
4. The container system of claim 3 , wherein the metal housing is configured to withstand a certain amount of pressure from the chromatography cartridge.
5. The container system of claim 1 , wherein the chromatography cartridge is disposed vertically in the housing.
6. The container system of claim 1 , further comprising chromatography media included in the chromatography cartridge.
7. The container system of claim 6 , wherein process liquids flow from a top portion of the chromatography cartridge to a bottom portion of the chromatograph cartridge.
8. The container system of claim 1 , wherein the chromatography cartridge is a pre-packed disposable cartridge.
9. The container system of claim 1 , wherein the amount of pressure is less than 3 bar.
10. The container system of claim 1 , wherein the housing is made of plastic.
11. A container system comprising:
a housing:
a chromatography cartridge disposed horizontally in the housing;
a structural support in the housing configured to retain the chromatography cartridge within the housing; and
the housing is configured to withstand a certain amount of pressure generated inside the chromatography cartridge.
12. The container system of claim 11 , wherein the chromatography cartridge includes a first mobile phase port and a second mobile phase port.
13. The container system of claim 11 , further comprising a chromatography media in the chromatography cartridge.
14. The container system of claim 13 , wherein the first mobile phase port is configured to receive process liquids.
15. The container system of claim 14 , wherein the second mobile phase port is configured to release process liquids.
16. A method for assembling a chromatography structure, comprising:
providing a housing, wherein the housing is configured to open, wherein the housing is configured to withstand a certain amount of pressure;
inserting a cartridge into the housing; and
securing the cartridge in the housing.
17. The method of claim 16 , wherein the housing is opened by opening a lid.
18. The method of claim 16 , wherein the housing is opened by unbolting a central flange.
19. The method of claim 16 , wherein the cartridge includes a storage solution.
20. The method of claim 19 , wherein the storage solution is selected from the group consisting of 20% ethanol, an antimicrobial agent, a weak benzyl alcohol and a weak caustic solution.
21. The method of claim 16 , wherein securing the cartridge in the housing further comprises of providing a plurality of ribs disposed in the housing to secure the cartridge in the housing.
22. The method of claim 20 , further comprising washing the storage solution out of the cartridge.
23. The method of claim 22 , further comprising providing water or buffer to wash the storage solution.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US12/663,750 US20100219115A1 (en) | 2007-06-27 | 2008-06-18 | System and method for assembling a large scale chromatography structure |
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Application Number | Priority Date | Filing Date | Title |
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US94640407P | 2007-06-27 | 2007-06-27 | |
US12/663,750 US20100219115A1 (en) | 2007-06-27 | 2008-06-18 | System and method for assembling a large scale chromatography structure |
PCT/US2008/067338 WO2009002780A1 (en) | 2007-06-27 | 2008-06-18 | System and method for assembling a large scale chromatograpy structure |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
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PCT/US2008/067338 A-371-Of-International WO2009002780A1 (en) | 2007-06-27 | 2008-06-18 | System and method for assembling a large scale chromatograpy structure |
Related Child Applications (1)
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US15/064,438 Continuation US11045746B2 (en) | 2007-06-27 | 2016-03-08 | System and method for assembling a large scale chromatography structure |
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US20100219115A1 true US20100219115A1 (en) | 2010-09-02 |
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US12/663,750 Abandoned US20100219115A1 (en) | 2007-06-27 | 2008-06-18 | System and method for assembling a large scale chromatography structure |
US15/064,438 Active 2029-10-12 US11045746B2 (en) | 2007-06-27 | 2016-03-08 | System and method for assembling a large scale chromatography structure |
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US15/064,438 Active 2029-10-12 US11045746B2 (en) | 2007-06-27 | 2016-03-08 | System and method for assembling a large scale chromatography structure |
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US (2) | US20100219115A1 (en) |
EP (1) | EP2160226B1 (en) |
WO (1) | WO2009002780A1 (en) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2015109246A1 (en) * | 2014-01-17 | 2015-07-23 | Repligen Corporation | Sterilizing chromatography columns |
US20180154280A1 (en) * | 2014-01-17 | 2018-06-07 | Repligen Corporation | Sterilizing chromatography columns |
US9993751B2 (en) | 2008-09-24 | 2018-06-12 | Repligen Corporation | Screen filter module for alternating flow filtration |
US10081788B2 (en) | 2010-08-25 | 2018-09-25 | Repligen Corporation | Device, system and process for modification or concentration of cell-depleted fluid |
US11156219B2 (en) | 2015-11-10 | 2021-10-26 | Repligen Corporation | Disposable alternating tangential flow filtration units |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2014102589A1 (en) | 2012-12-27 | 2014-07-03 | Purdue Pharma L.P. | Quinazolin-4(3h)-one-type piperidine compounds and uses thereof |
AU2018385394B2 (en) * | 2017-12-13 | 2024-05-02 | Regeneron Pharmaceuticals, Inc. | Devices and systems for chromatography column bed support management and related methods |
Citations (30)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2323830A (en) * | 1941-01-27 | 1943-07-06 | Shell Dev | Catalytic conversion process |
US3265214A (en) * | 1963-04-15 | 1966-08-09 | Esco Products Inc | Chromatography cabinet |
US4151092A (en) * | 1977-07-11 | 1979-04-24 | Teledyne Industries, Inc. | Portable water filter |
US4350586A (en) * | 1981-09-25 | 1982-09-21 | The Perkin-Elmer Corporation | Apparatus useful with a chromatography column |
US4496461A (en) * | 1983-06-17 | 1985-01-29 | Amf Incorporated | Chromatography column |
US4578187A (en) * | 1984-06-29 | 1986-03-25 | Alhaeuser Erich | Ion exchange apparatus to improve the quality of potable water |
US4627918A (en) * | 1985-11-04 | 1986-12-09 | Sepragen Corporation | Chromatography column using horizontal flow |
US4692246A (en) * | 1986-04-17 | 1987-09-08 | Simon Abraham E | Cartridge filter with plural medias |
US5221473A (en) * | 1989-10-13 | 1993-06-22 | Burrows Bruce D | Filter cartridge assembly for a reverse osmosis purification system |
US5431813A (en) * | 1994-02-14 | 1995-07-11 | Daniels; Jack E. | Water filtering bottle |
US5562824A (en) * | 1994-10-12 | 1996-10-08 | Wtc/Ecomaster Corporation | Gravity water purifier |
US6001253A (en) * | 1995-05-16 | 1999-12-14 | Dyax Corporation | Liquid chromatography column |
US6136189A (en) * | 1998-01-20 | 2000-10-24 | Innova Pure Water Inc. | Enhanced in-bottle filtration mechanism and techniques |
US6444122B1 (en) * | 1997-11-14 | 2002-09-03 | Dyax Corporation | Liquid chromatography column |
US20030098280A1 (en) * | 1994-10-03 | 2003-05-29 | John Davis | Access valve devices, their use in separation apparatus, and corresponding methods |
US20030102266A1 (en) * | 2002-09-20 | 2003-06-05 | Ritacco Robert P. | Process flash chromatography column |
US20030173279A1 (en) * | 2002-03-15 | 2003-09-18 | Giacomo Aste | Chromatographic apparatus |
US20040129617A1 (en) * | 2001-08-23 | 2004-07-08 | Pur Water Purification Products, Inc. | Water filter device |
US20040164012A1 (en) * | 2003-02-24 | 2004-08-26 | Dunkley John Graham | Chromatography column |
US20060011532A1 (en) * | 2004-07-16 | 2006-01-19 | Van Davelaar Peter C | Chromatography cartridge |
US6998273B1 (en) * | 2000-02-09 | 2006-02-14 | A-Fem Medical Corporation | Collection device for lateral flow chromatography |
US7001550B2 (en) * | 1999-07-30 | 2006-02-21 | Genentech, Inc. | Charged filtration membranes and uses therefor |
US20060070950A1 (en) * | 2004-10-01 | 2006-04-06 | 3M Innovative Properties Company | Composite filtration article |
US7074331B2 (en) * | 2002-06-26 | 2006-07-11 | Dionex Corporation | Separation system, components of a separation system and methods of making and using them |
US20060213822A1 (en) * | 2005-03-22 | 2006-09-28 | Analogix, Inc. | Method and apparatus for injecting a sample into a chromatography system |
US20060273012A1 (en) * | 2005-03-02 | 2006-12-07 | Agilent Technologies, Inc. | Column with additional fluid introduction |
US20070119770A1 (en) * | 2005-11-29 | 2007-05-31 | Koji Suzuki | Ion exchange filter |
US20080164195A1 (en) * | 2004-03-19 | 2008-07-10 | Millipore Corporation | Prefilter system for biological systems |
US7452464B2 (en) * | 2004-11-02 | 2008-11-18 | Ge Healthcare Bio-Sciences Ab | Chromatography column |
US20090230045A1 (en) * | 2005-05-24 | 2009-09-17 | Hellerman Tyton Co., Ltd | Column and cartridge column using the same |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2640115C2 (en) * | 1975-09-29 | 1986-06-19 | Teledyne Industries, Inc., Los Angeles, Calif. | Device for water purification |
US4676898A (en) * | 1985-11-04 | 1987-06-30 | Sepragen Corporation | Chromatography column using horizontal flow |
FR2681138A1 (en) * | 1991-09-09 | 1993-03-12 | Couillard Francois | Improvements to chromatography columns |
US20080257838A1 (en) * | 2007-04-23 | 2008-10-23 | Gene Hirs | Cartridge based filter assembly for use in removing sub micronic particles from fluids |
CN204699732U (en) * | 2015-06-10 | 2015-10-14 | 中国农业科学院兰州畜牧与兽药研究所 | A kind of chromatographic column case |
-
2008
- 2008-06-18 WO PCT/US2008/067338 patent/WO2009002780A1/en active Application Filing
- 2008-06-18 US US12/663,750 patent/US20100219115A1/en not_active Abandoned
- 2008-06-18 EP EP08771360.8A patent/EP2160226B1/en active Active
-
2016
- 2016-03-08 US US15/064,438 patent/US11045746B2/en active Active
Patent Citations (31)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2323830A (en) * | 1941-01-27 | 1943-07-06 | Shell Dev | Catalytic conversion process |
US3265214A (en) * | 1963-04-15 | 1966-08-09 | Esco Products Inc | Chromatography cabinet |
US4151092A (en) * | 1977-07-11 | 1979-04-24 | Teledyne Industries, Inc. | Portable water filter |
US4350586A (en) * | 1981-09-25 | 1982-09-21 | The Perkin-Elmer Corporation | Apparatus useful with a chromatography column |
US4675104A (en) * | 1983-06-17 | 1987-06-23 | Cuno Inc. | Chromatography column |
US4496461A (en) * | 1983-06-17 | 1985-01-29 | Amf Incorporated | Chromatography column |
US4578187A (en) * | 1984-06-29 | 1986-03-25 | Alhaeuser Erich | Ion exchange apparatus to improve the quality of potable water |
US4627918A (en) * | 1985-11-04 | 1986-12-09 | Sepragen Corporation | Chromatography column using horizontal flow |
US4692246A (en) * | 1986-04-17 | 1987-09-08 | Simon Abraham E | Cartridge filter with plural medias |
US5221473A (en) * | 1989-10-13 | 1993-06-22 | Burrows Bruce D | Filter cartridge assembly for a reverse osmosis purification system |
US5431813A (en) * | 1994-02-14 | 1995-07-11 | Daniels; Jack E. | Water filtering bottle |
US20030098280A1 (en) * | 1994-10-03 | 2003-05-29 | John Davis | Access valve devices, their use in separation apparatus, and corresponding methods |
US5562824A (en) * | 1994-10-12 | 1996-10-08 | Wtc/Ecomaster Corporation | Gravity water purifier |
US6001253A (en) * | 1995-05-16 | 1999-12-14 | Dyax Corporation | Liquid chromatography column |
US6444122B1 (en) * | 1997-11-14 | 2002-09-03 | Dyax Corporation | Liquid chromatography column |
US6136189A (en) * | 1998-01-20 | 2000-10-24 | Innova Pure Water Inc. | Enhanced in-bottle filtration mechanism and techniques |
US7001550B2 (en) * | 1999-07-30 | 2006-02-21 | Genentech, Inc. | Charged filtration membranes and uses therefor |
US6998273B1 (en) * | 2000-02-09 | 2006-02-14 | A-Fem Medical Corporation | Collection device for lateral flow chromatography |
US20040129617A1 (en) * | 2001-08-23 | 2004-07-08 | Pur Water Purification Products, Inc. | Water filter device |
US20030173279A1 (en) * | 2002-03-15 | 2003-09-18 | Giacomo Aste | Chromatographic apparatus |
US7074331B2 (en) * | 2002-06-26 | 2006-07-11 | Dionex Corporation | Separation system, components of a separation system and methods of making and using them |
US20030102266A1 (en) * | 2002-09-20 | 2003-06-05 | Ritacco Robert P. | Process flash chromatography column |
US20040164012A1 (en) * | 2003-02-24 | 2004-08-26 | Dunkley John Graham | Chromatography column |
US20080164195A1 (en) * | 2004-03-19 | 2008-07-10 | Millipore Corporation | Prefilter system for biological systems |
US20060011532A1 (en) * | 2004-07-16 | 2006-01-19 | Van Davelaar Peter C | Chromatography cartridge |
US20060070950A1 (en) * | 2004-10-01 | 2006-04-06 | 3M Innovative Properties Company | Composite filtration article |
US7452464B2 (en) * | 2004-11-02 | 2008-11-18 | Ge Healthcare Bio-Sciences Ab | Chromatography column |
US20060273012A1 (en) * | 2005-03-02 | 2006-12-07 | Agilent Technologies, Inc. | Column with additional fluid introduction |
US20060213822A1 (en) * | 2005-03-22 | 2006-09-28 | Analogix, Inc. | Method and apparatus for injecting a sample into a chromatography system |
US20090230045A1 (en) * | 2005-05-24 | 2009-09-17 | Hellerman Tyton Co., Ltd | Column and cartridge column using the same |
US20070119770A1 (en) * | 2005-11-29 | 2007-05-31 | Koji Suzuki | Ion exchange filter |
Non-Patent Citations (1)
Title |
---|
PTO Translation No. 12-3140 of French Patent No. 2,681,138, pages 1-14, April 2012 * |
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US11225637B2 (en) | 2010-08-25 | 2022-01-18 | Repligen Corporation | Enclosed filtration system processes |
WO2015109246A1 (en) * | 2014-01-17 | 2015-07-23 | Repligen Corporation | Sterilizing chromatography columns |
CN105916561A (en) * | 2014-01-17 | 2016-08-31 | 瑞普利根公司 | Sterilizing chromatography columns |
KR20160108355A (en) * | 2014-01-17 | 2016-09-19 | 리플리겐 코포레이션 | Sterilizing chromatography columns |
JP2017509866A (en) * | 2014-01-17 | 2017-04-06 | レプリゲン・コーポレイションRepligen Corporation | Sterilization of chromatography columns |
US20180154280A1 (en) * | 2014-01-17 | 2018-06-07 | Repligen Corporation | Sterilizing chromatography columns |
US10610807B2 (en) | 2014-01-17 | 2020-04-07 | Repligen Corporation | Sterilizing chromatography columns |
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Also Published As
Publication number | Publication date |
---|---|
WO2009002780A1 (en) | 2008-12-31 |
US20160228791A1 (en) | 2016-08-11 |
EP2160226A1 (en) | 2010-03-10 |
EP2160226B1 (en) | 2015-11-11 |
US11045746B2 (en) | 2021-06-29 |
EP2160226A4 (en) | 2011-12-14 |
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