US20100078323A1 - Component separating device and chemical analysis device using the same - Google Patents
Component separating device and chemical analysis device using the same Download PDFInfo
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- US20100078323A1 US20100078323A1 US12/444,485 US44448507A US2010078323A1 US 20100078323 A1 US20100078323 A1 US 20100078323A1 US 44448507 A US44448507 A US 44448507A US 2010078323 A1 US2010078323 A1 US 2010078323A1
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- 239000000126 substance Substances 0.000 title claims description 22
- 239000000758 substrate Substances 0.000 claims abstract description 46
- 239000007787 solid Substances 0.000 claims description 34
- 239000007788 liquid Substances 0.000 claims description 20
- 239000000203 mixture Substances 0.000 claims description 13
- 238000006243 chemical reaction Methods 0.000 claims description 10
- 238000012360 testing method Methods 0.000 claims description 9
- 238000000926 separation method Methods 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 5
- 239000008280 blood Substances 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 5
- 239000012141 concentrate Substances 0.000 description 5
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 229910052710 silicon Inorganic materials 0.000 description 4
- 239000010703 silicon Substances 0.000 description 4
- 238000006073 displacement reaction Methods 0.000 description 3
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 229910052719 titanium Inorganic materials 0.000 description 2
- 239000010936 titanium Substances 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 238000009534 blood test Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000002542 deteriorative effect Effects 0.000 description 1
- 238000001312 dry etching Methods 0.000 description 1
- 239000010419 fine particle Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- HFGPZNIAWCZYJU-UHFFFAOYSA-N lead zirconate titanate Chemical compound [O-2].[O-2].[O-2].[O-2].[O-2].[Ti+4].[Zr+4].[Pb+2] HFGPZNIAWCZYJU-UHFFFAOYSA-N 0.000 description 1
- 229910052451 lead zirconate titanate Inorganic materials 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 239000012798 spherical particle Substances 0.000 description 1
- 238000004544 sputter deposition Methods 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D21/00—Separation of suspended solid particles from liquids by sedimentation
- B01D21/28—Mechanical auxiliary equipment for acceleration of sedimentation, e.g. by vibrators or the like
- B01D21/283—Settling tanks provided with vibrators
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D21/00—Separation of suspended solid particles from liquids by sedimentation
- B01D21/30—Control equipment
- B01D21/32—Density control of clear liquid or sediment, e.g. optical control ; Control of physical properties
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2221/00—Applications of separation devices
- B01D2221/10—Separation devices for use in medical, pharmaceutical or laboratory applications, e.g. separating amalgam from dental treatment residues
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0652—Sorting or classification of particles or molecules
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
- B01L2400/0439—Moving fluids with specific forces or mechanical means specific forces vibrational forces ultrasonic vibrations, vibrating piezo elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
Landscapes
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Health & Medical Sciences (AREA)
- Hematology (AREA)
- Dispersion Chemistry (AREA)
- Fluid Mechanics (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Clinical Laboratory Science (AREA)
- Piezo-Electric Or Mechanical Vibrators, Or Delay Or Filter Circuits (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Separation Of Solids By Using Liquids Or Pneumatic Power (AREA)
Abstract
Description
- The present invention relates to a small component separating device for separating a liquid, such as blood or emulsion, into a liquid component and a solid component, and to a chemical analysis device chemically analyzing a test substance using the device.
- A micro total analysis system which has been paid attention for as a next-generation analysis technique refers to a micrometer-scale, chemical analysis device integrating processes for introducing a test substance, i.e., a mixture of liquid and solid components, such as blood, for transferring the substance to a component separating device to separate the liquid into the components, for causing the components react with reagents, and for analyzing the reaction.
-
FIG. 18 is a sectional view of a conventional component separating device. As shown inFIG. 18 , conventionalcomponent separating device 1 includessubstrate 2,seal 3 covering abovesubstrate 2, andvibrator 4 provided on a side ofsubstrate 2.Substrate 2 haschannel groove 5 therein for transferring a test substance.Vibrator 4 including a piezoelectric element generates an acoustic wave with a predetermined frequency.Vibrator 4 causes a solid component to concentrate at a node of an acoustic standing wave generated inchannel groove 5 to separate various components. Such separation methods using acoustic waves are described inNon-patent Documents - However, in conventional
component separating device 1 described above,vibrator 4 requires a high driving voltage. This is because vibration fromvibrator 4 diffuses toentire substrate 2 and seal 3 to attenuate the standing wave generated inchannel groove 5, accordingly preventing the component from being separated accurately. - Non-patent Document 1: Carl Siversson, Micro Total Analysis Systems 2004, pp 330-332, vol. 2
- Non-patent Document 2: Holden Li, Micro Total Analysis Systems 2004, pp 12-14, vol. 1
- According to the present invention, the strength of a standing wave is increased even with a low driving voltage to
vibrator 4. A component separating device according to the present invention includes a projection provided on an outer side wall opposite to a channel groove. A vibrator provides this projection with warping vibration in a depth direction of the channel groove. This component separating device increases the strength of a standing wave even with a low driving voltage applied to the vibrator. The warping vibration of the projection is amplified due to an effect of leverage, thereby generating a large stress at the outer side wall of the channel groove having the projection thereon. Thus, the device increases the strength of the standing wave inside the channel groove even with the low driving voltage, thereby separating the component accurately. -
FIG. 1 is an exploded perspective view of a component separating device according to the present invention. -
FIG. 2 is a sectional schematic view of a vibrator according to the invention. -
FIG. 3 is a schematic sectional view of the component separating device according to the invention for illustrating an operation of the device. -
FIG. 4 is a schematic sectional view of a channel groove according to the invention. -
FIG. 5 is a schematic top view of the channel groove according to the invention. -
FIG. 6 is an exploded perspective view of the component separating device according to the invention. -
FIG. 7 is an exploded perspective view of the component separating device according to the invention. -
FIG. 8 is a schematic sectional view of the component separating device according to the invention for illustrating an operation of the device. -
FIG. 9 is an exploded perspective view of the component separating device according to the invention. -
FIG. 10 is an exploded perspective view of the component separating device according to the invention. -
FIG. 11 is an exploded perspective view of the component separating device according to the invention. -
FIG. 12 is a schematic top view of the component separating device according to the invention. -
FIG. 13 is a schematic top view of the component separating device according to the invention. -
FIG. 14 is an exploded perspective view of the component separating device according to the invention. -
FIG. 15 is a perspective view of a substrate and a projection according to the invention. -
FIG. 16 is a schematic sectional view of the component separating device according to the invention for illustrating an operation of the device. -
FIG. 17 is a perspective view of a chemical analysis device according to the invention. -
FIG. 18 is a sectional view of a conventional component separating device. -
- 6 Component Separating Device
- 7 Channel Groove
- 7 a, 7 b Outer Side Wall
- 8 Substrate
- 9 Seal
- 10, 10 a to 10 e Projection
- 11, 11 a to 11 e Vibrator
- 12 First Electrode
- 13 Piezoelectric Body
- 14 Second Electrode
- 15 Liquid Component
- 16, 16 a, 16 b Solid Component
- 17 Standing Wave
- 18 Node
- 19 a Warping Vibration
- 19 b Acoustic Wave Vibration
- 20 a, 20 b Flow
- 21 Chemical Analysis Device
- 22 Test Substance Inlet
- 23 Transfer Section
- 24 Reaction Section
- 25 Analysis Section
- 26 Silicon Substrate
- 27 Space
-
Component separating device 6 according toExemplary Embodiment 1 of the present invention will be described below.FIG. 1 shows an exploded perspective view ofcomponent separating device 6 according to the embodiment.Component separating device 6 according to the embodiment shown inFIG. 1 includessubstrate 8 havingchannel groove 7 opening in a upper surface of the substrate,seal 9 provided abovesubstrate 8 so as to cover the opening ofchannel groove 7,projection 10 provided onouter side wall 7 a of the substrate opposite to channelgroove 7, andvibrator 11 provided on an upper surface ofprojection 10.Substrate 8 andseal 9 are joined together with an adhesive. According to this embodiment,substrate 8 andseal 9 are made of silicon and glass, respectively.Seal 9 may be made of plastic or silicon besides glass. -
FIG. 2 is a schematic sectional view ofvibrator 11. As shown inFIG. 2 ,vibrator 11 includesfirst electrode 12 made of titanium or platinum,piezoelectric body 13 made of lead zirconate titanate, andsecond electrode 14 made of titanium or gold in the order from projection 10 (shown inFIG. 1 ). An alternating-current (AC) voltage is applied betweenfirst electrode 12 andsecond electrode 14 to causeprojection 10 to warping and vibrating in a depth direction ofchannel groove 7. The vibrator made of these materials converts electric energy to mechanical energy efficiently, thereby being displaced even if with the voltage driving the vibrator is low. -
FIG. 3 is a schematic sectional view ofcomponent separating device 6 for illustrating an operation of the device. As shown inFIG. 3 ,channel groove 7 has a predetermined width and a predetermined depth so as to contain a mixture ofliquid component 15 andsolid component 16 therein and to allow the mixture through the groove. The width ofchannel groove 7 is determined to generate a predetermined standing wave inchannel groove 7.FIG. 4 shows the channel groove. Standingwave 17 is represented by the dotted lines inFIG. 4 . - A method of designing
channel groove 7 will be described below with reference toFIG. 4 .Channel groove 7 has width W. A speed of sound inliquid component 15 out of a mixture ofliquid component 15 andsolid component 16 introduced tochannel groove 7 is v. An acoustic wave with frequency f satisfying: -
f=(n/2)×v/W (where n is a natural number) - is applied to
channel groove 7 to generate standingwave 17 inchannel groove 7. - According to this embodiment,
projection 10 has a shape having frequency f as its primary resonance frequency. -
Substrate 8 andprojection 10 are made of a single substrate. This structure maintains the strength ofsubstrate 8 andprojection 10 even if a portion at whichsubstrate 8 is connected withprojection 10 has a stress due to warpingvibration 19 a ofprojection 10. InFIG. 3 arrow 19 a represents the direction of warpingvibration 19 a. - An operation of
component separating device 6 according to the embodiment will be described below. First, as shown inFIG. 3 , a mixture ofliquid component 15 andsolid component 16 is introduced intochannel groove 7. When a driving voltage providing vibration with frequency f is applied tovibrator 11,projection 10 warps and vibrates as warpingvibration 19 a so as to have its shape change as shown by the dotted lines inFIG. 3 in response to this driving voltage.Projection 10 is shorter thansubstrate 8 in a longitudinal direction of the substrate, hence allowingprojection 10 to deform and vibrate easily. Sinceouter side wall 7 a opposite to channelgroove 7 is connected withprojection 10, vibration caused by warpingvibration 19 a ofprojection 10 propagates, as an acoustic wave, toouter side wall 7 a ofchannel groove 7.Arrow 19 b inFIG. 3 represents the vibration due to this acoustic wave. According to this embodiment,projection 10 andouter side wall 7 a are made of a single substrate, and allows the acoustic wave to efficiently propagate with a low propagation resistance. Then, this acoustic wave generates standingwave 17 inchannel groove 7, as shown inFIG. 4 . Standingwave 17 applies a force tosolid component 16 in a direction towardnode 18 of standingwave 17, thereby allowingsolid component 16 to concentrate tonode 18. -
FIG. 5 is a schematic top view of a channel according to the invention. As shown in a top view ofchannel groove 7 inFIG. 5 , the mixture is separated intoliquid component 15 andsolid component 16 inchannel groove 7, and then flow 20 a mainly containingliquid component 15 andflow 20 b ofsolid component 16 highly concentrating are divided at a branch ofchannel groove 7, thus extractingcomponent 15 andsolid component 16 separately. -
Projection 10 has a primary resonance frequency equal to frequency f, hence producing warpingvibration 19 a with a large displacement more efficiently than a projection having other shapes. This structure provides standingwave 17 with a large strength, providingcomponent separating device 6 with a small size. - Effects according to the embodiment will be described below.
Component separating device 6 according to this embodiment can increase the strength of standingwave 17 even with a low driving voltage tovibrator 11. More specifically, in conventionalcomponent separating device 1,vibrator 4 is directly stuck on the side ofsubstrate 2, as shown inFIG. 18 . According to this embodiment, on the other hand,channel groove 7 hasprojection 10 onouter side wall 7 a opposite to channelgroove 7, as shown inFIG. 3 .Projection 10 hasvibrator 11 thereon. According to this embodiment, warpingvibration 19 a ofprojection 10 propagates as an acoustic wave intensively toouter side wall 7 a opposite to channelgroove 7 havingprojection 10 provided thereon. At this moment, the vibration is amplified due to effect of leverage, thereby producing a large stress atouter side wall 7 a opposite to channelgroove 7, which is a supporting point and a working point of the leverage. Consequently, the strength of standingwave 17 inchannel groove 7 increases even for a low driving voltage and for asmall vibrator 11, thereby improving the component-separation accuracy. The vibration propagates toentire substrate 8 andseal 9, and attenuates. However, according to this embodiment, the strength of standingwave 17 is previously increased, accordingly preventing separation accuracy from deteriorating. - The width and arrangement of
projections 10 may be appropriately adjusted to generate standingwave 17 at a predetermined position ofchannel groove 7, thereby improving the accuracy of component separation. -
Vibrator 11 according to this embodiment having a laminated structure with superior adhesion maintains high durability even against repetitive displacement.Vibrator 11 according to this embodiment made of the aforementioned materials can be pattern-molded accurately by, e.g. dry etching onprojection 10 after sputtering. This process formsvibrator 11 accurately at a desired portion ofchannel groove 7 where standingwave 17 is generated. -
FIG. 6 is an exploded perspective view of anothercomponent separating device 6 according to the present invention. In the above mentioned device,vibrator 11 is provided on the upper surface ofprojection 10. As shown inFIG. 6 ,vibrator 11 may be provided on a lower surface of the projection.Vibrator 11 provided on the lower surface can be wired arbitrarily without interfering withchannel groove 7 andseal 9. -
FIG. 7 is an exploded perspective view ofcomponent separating device 6 according toExemplary Embodiment 2 of the present invention. The device according toEmbodiment 2 is different from the device according toEmbodiment 1 in that twovibrators projection 10, as shown inFIG. 7 , and that the shape ofprojection 10 is designed so that frequency f is a secondary resonance frequency of the projection.FIG. 8 schematically shows the relationship between the shape ofprojection 10 and the resonance frequency represented by curved dotted lines. - According to this embodiment, this structure increases the strength of standing
wave 17 generated inchannel groove 7, thereby improving the separation accuracy ofcomponent separating device 6. In the case that frequency f is extremely high, only onesmall vibrator 11 can be provided on the projection to use a primary resonance of the warping vibration ofprojection 10, hence causing generatingweak standing wave 17. If only one large vibrator is provided to use higher-order resonance, the resonance is not produced efficiently since the piezoelectric body can hardly deform. According to this embodiment,plural vibrators 11 arranged onprojection 10 produce higher-order resonance atprojection 10 efficiently, thus generatingstanding wave 17 with sufficient strength. - For example, according to this embodiment, driving voltages having frequency f and having phases different from each other by 180 degrees are applied to
vibrators 11 a and lib, respectively, as shown inFIG. 8 , thereby producing warpingvibration 19 a of the secondary resonance efficiently. Consequently, the mixture ofliquid component 15 andsolid component 16 can be separated and extracted efficiently intoliquid component 15 andsolid component 16 even for extremely high frequency f similarly to the device according toEmbodiment 1. The number ofvibrators 11 may be changed to adjust the strength of standingwave 17 over a wide range. -
FIG. 9 is an exploded perspective view ofcomponent separating device 6 according toExemplary Embodiment 3 of the present invention. The device according toEmbodiment 3 is different from the device according toEmbodiment 1 in thatplural projections 10 a to 10 c having shapes identical to each other are provided onouter side wall 7 a of one side of the substrate opposite to channelgroove 7, and thatvibrators 11 a to 11 c are provided on upper surfaces ofprojections 10 a to 10 c, respectively, as shown inFIG. 9 . This structure generates standingwave 17 in a large area, accordingly causingsolid component 16 to concentrate sufficiently even ifsolid component 16 is too small to receive a large force due to a sound pressure of the standing wave for concentrating. Further, this device can suppress side-effect vibrations more than adevice including projection 10 having an elongated side parallel tochannel groove 7, accordingly enabling more efficient component separation. -
FIG. 10 is an exploded perspective view ofcomponent separating device 6 having another shape. InFIG. 9 ,projections 10 are provided onouter side wall 7 a at one side opposite to channelgroove 7. InFIG. 10 ,projections 10 are provided onouter side walls groove 7. In this case, acoustic waves propagating from both sides ofchannel groove 7 generates standingwave 17 with large strength. -
FIG. 11 is an exploded perspective view ofcomponent separating device 6 according toExemplary Embodiment 4. The device according toEmbodiment 4 is different from the device according toEmbodiment 1 in that twoprojections outer side wall 7 a of the substrate opposite to channelgroove 7, as shown inFIG. 11 . -
FIG. 12 is a schematic top view ofchannel groove 7. Smallsolid component 16 a and largesolid component 16 b are mixed withliquid component 15. According to this embodiment, the shape offirst projection 10 d is determined so that frequency f1 satisfying -
f1=n×v/W (n is a natural number) - is a resonance frequency of warping
vibration 19 a, where W is the width ofgroove 7, and v is a speed of sound inliquid component 15 out of the mixture ofliquid component 15 andsolid components channel groove 7. - The shape of
second projection 10 e is determined so that frequency f2 satisfying: -
f2=(½)×v/W; or -
f2=(½+n)×v/W (n is a natural number) - is the resonance frequency of warping
vibration 19 a.Vibrators projections - In the above-described structure, standing
waves 17 withnodes 18 of which numbers are different from each other are generated, as shown inFIG. 12 , thereby separatingsolid components 16 with different properties from each other. - A separation using
component separating device 6 according to this embodiment will be described below. First, as shown in the top view ofchannel groove 7 ofFIG. 12 , the mixture ofliquid component 15, smallsolid component 16 a, and largesolid component 16 b is introduced tochannel groove 7. When a driving voltage with frequency f3 satisfying -
f3=v/W - is applied to
vibrator 11 d, twonodes 18 of standingwave 17 are generated in an area ofchannel groove 7 facingvibrator 11 d. In this case, if the driving voltage is increased, both smallsolid component 16 a and largesolid component 16 b sufficiently concentrate. - When a driving voltage with frequency f4 satisfying
-
f4=(½)×v/W - is applied to
vibrator 11 e,single node 18 of standingwave 17 is generated in an area ofchannel groove 7 facingvibrator 11 e. At this moment, if the driving voltage is decreased, only largesolid component 16 b concentrates. - It is known that, if
solid components wave 17 is proportional to the cube of the particle diameter of the particles, namely, to the volume of each particle. The area where standingwave 17 is generated and its strength may be controlled to change the density of particles concentrating tonode 18 of standingwave 17 according to the sizes ofsolid components - Thus, the device according to this embodiment separates the mixture into
flow 20 a of highly-concentrating smallsolid component 16 a andflow 20 b of highly-concentrating largesolid component 16 b. As shown inFIG. 13 , flows 20 a and 20 b are divided by a branch ofchannel groove 7 to extract smallsolid component 16 a and largesolid component 16 b. -
FIG. 14 is an exploded perspective view ofcomponent separating device 6 having another shape. As shown inFIG. 14 ,projections outer side walls groove 7, respectively. In this structure, vibration sources are provided on the outer walls facing each other, and reduce interference of the respective frequencies even whenprojection 10 a andprojection 10 b are driven simultaneously to each other. -
FIG. 15 is a perspective view ofsubstrate 8 andprojection 10 according toExemplary Embodiment 5 of the present invention. As shown inFIG. 15 , the device according to this embodiment is different from the device according toEmbodiment 2 in thatplural channel grooves 7 are provided, and thatprojections 10 provided onouter side walls 7 a of the substrate which are opposite to channelgrooves 7 and face each other are adjacent to each other. - According to this embodiment,
vibrators 11 are provided onprojections 10. Even ifvibrators 11 are adjacent to each other, as shown in the figure, the vibrators have spaces between them, thereby suppressing interference of respective acoustic waves.Plural vibrators 11 can be positioned close to each other, and thus the space inside the device is effectively used. - The device according to this embodiment is applicable not only to the device having
plural channel grooves 7, but also to the device havingchannel groove 7 is curved and branched as well since portions ofchannel groove 7 may face each other. In these cases,projections 10 arranged on the side walls of the portions ofchannel groove 7 adjacent to each other can be adjacent to each other as well, thereby suppressing interference of acoustic waves in a small space. -
FIG. 16 is a schematic sectional view ofcomponent separating device 6 according toExemplary Embodiment 6 of the present invention for illustrating an operation of the device. As shown inFIG. 16 , the device according to this embodiment is different from the device according toEmbodiment 1 in thatprojection 10 has a thickness smaller than that ofsubstrate 8 and substantially identical to the depth ofchannel groove 7. This structure increases the displacement of warpingvibration 19 a and the strength of standingwave 17 generated inchannel groove 7. The depth ofchannel groove 7 is substantially identical to the thickness ofprojection 10. This arrangement allows vibration to propagate intensively tochannel groove 7, thereby further increasing the strength of standingwave 17 generated inchannel groove 7. -
FIG. 17 showschemical analysis device 21 for a blood test according to this embodiment includingcomponent separating device 6 according toEmbodiment 1 described above.Chemical analysis device 21 includestest substance inlet 22, transfer section (pump) 23 connected withtest substance inlet 22,component separating device 6 connected withtransfer section 23,reaction section 24 connected withcomponent separating device 6, andanalysis section 25 connected withreaction section 24. - Samples blood, upon being put into
test substance inlet 22, is transferred tocomponent separating device 6 throughtransfer section 23, and then is separated into the components of the blood through channel groove 7 (not shown). When each component reaches eachreaction section 24, a reagent is put intoreaction section 24 to start a chemical reaction. Then,analysis section 25 reads data on this chemical reaction.Chemical analysis device 21 according to this embodiment is made ofsilicon substrate 26 having a square shape having sides ranging from 20 mm to 30 mm as a base. -
Chemical analysis device 21 hasspace 27 provided aroundprojection 10 ofcomponent separating device 6. This space prevents vibration of the vibrator (not shown) onprojection 10 from diffusing into surroundings, thereby increasing the strength of standingwave 17 generated insidechannel groove 7. -
Space 27 aroundprojection 10 reduces the weight of the device. -
Chemical analysis device 21 according to the embodiment may includecomponent separating device 6 according to any one ofEmbodiments 2 to 6, providing the same effects.Chemical analysis device 21 particularly includingcomponent separating device 6 according toEmbodiment 6 havespace 27 formed above and belowprojection 10, thus reducing the weight of the entire device and reducing the attenuation of acoustic waves. - According to the present invention, components of a mixed solution, such as blood or emulsion, of liquid component and solid component can be separated into the components, and thus useful for a component separator and a component analyzer.
Claims (15)
f=(n/2)×v/W,
f1=n×v/W, and
f2=(½)×v/W or f2=(½+n)×v/W.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
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JP2006318239 | 2006-11-27 | ||
JP2006-318239 | 2006-11-27 | ||
JP2006318239A JP4984849B2 (en) | 2006-11-27 | 2006-11-27 | Component separation device and chemical analysis device using the component separation device |
PCT/JP2007/072173 WO2008065897A1 (en) | 2006-11-27 | 2007-11-15 | Component separating device and chemical analysis device using the same |
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US20100078323A1 true US20100078323A1 (en) | 2010-04-01 |
US8080202B2 US8080202B2 (en) | 2011-12-20 |
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US12/444,485 Expired - Fee Related US8080202B2 (en) | 2006-11-27 | 2007-11-15 | Component separating device and chemical analysis device using the same |
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US (1) | US8080202B2 (en) |
EP (1) | EP2053411B1 (en) |
JP (1) | JP4984849B2 (en) |
WO (1) | WO2008065897A1 (en) |
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Also Published As
Publication number | Publication date |
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EP2053411B1 (en) | 2015-07-15 |
EP2053411A4 (en) | 2013-06-05 |
US8080202B2 (en) | 2011-12-20 |
JP4984849B2 (en) | 2012-07-25 |
JP2008134063A (en) | 2008-06-12 |
WO2008065897A1 (en) | 2008-06-05 |
EP2053411A1 (en) | 2009-04-29 |
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