US20100069477A1 - Methioninase inhibitor and composition and food or drink containing the same - Google Patents
Methioninase inhibitor and composition and food or drink containing the same Download PDFInfo
- Publication number
- US20100069477A1 US20100069477A1 US12/309,645 US30964507A US2010069477A1 US 20100069477 A1 US20100069477 A1 US 20100069477A1 US 30964507 A US30964507 A US 30964507A US 2010069477 A1 US2010069477 A1 US 2010069477A1
- Authority
- US
- United States
- Prior art keywords
- myrsinoic
- extract
- myrsinoic acid
- ethanol
- methioninase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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Classifications
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- A61K31/34—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having five-membered rings with one oxygen as the only ring hetero atom, e.g. isosorbide
- A61K31/343—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having five-membered rings with one oxygen as the only ring hetero atom, e.g. isosorbide condensed with a carbocyclic ring, e.g. coumaran, bufuralol, befunolol, clobenfurol, amiodarone
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- A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline
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- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
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- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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- A61K2800/74—Biological properties of particular ingredients
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- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
Definitions
- the present invention relates to an inhibitor of methioninase engaged in production of methyl mercaptan that is a causative substance of a bad smell, as well as a composition and a food or drink containing the same.
- the main components of a bad smell from an oral cavity are volatile sulfur compounds, and especially methyl mercaptan is known to have a high correlation with the intensity of bad breath.
- Methyl mercaptan is generated from food debris, intraoral desquamated epithelial cells and salivary proteins metabolized or decomposed by oral bacteria.
- Volatile sulfur compounds such as methyl mercaptan are known to have adverse effects, such as inhibition of intraoral synthesis of proteins, inhibition of collagen synthesis, inhibition of growth and division of endothelial cells, and increase of permeability of intraoral mucosa. Consequently inhibition of methyl mercaptan is an important task not only for suppression of the bad breath, but also for prevention of a periodontal disease or maintenance of the human body's internal environment.
- Methyl mercaptan is generated from L-methionine, that is an intraoral protein decomposition product, utilized as a substrate, by an oral bacterial enzyme of methioninase (L-methionine- ⁇ -lyase).
- L-methionine- ⁇ -lyase an oral bacterial enzyme of methioninase
- Fusobacterium nucleatum and Porphyromonas gingivalis have high methioninase activity, and by inhibiting their methioninase activity the generation of methyl mercaptan, a causative substance of bad smell, as well as hydrogen sulfide, ammonia and ⁇ -ketobutyrate can be inhibited.
- Methyl mercaptan is generated also by enteric bacteria, and known as a causative substance of odors of kitchen garbage and feces. Consequently, by inhibiting methioninase, the odors of garbage and feces are expected to be eliminated.
- the use of a disinfectant might have an adverse effect by disturbing a balance of an intraoral bacterial flora, and the odor eliminating by chemical conversion or masking does not inhibit generation of the odor itself and the effect does not last long.
- the methioninase inhibitor of the present invention is safe, inhibits the generation of methyl mercaptan itself and therefore shows high sustainability.
- Methioninase inhibitors originated from natural materials, such as a tomato extract and a ginger extract (e.g. Patent Documents 1 and 2), or an extract of Iceland moss, an extract of alkanet, an extract of green tea and the like (e.g. Patent Document 3) have been reported, and their inhibition of the methyl mercaptan production by oral bacteria, such as Porphyromonas gingivalis and Fusobacterium nucleatum , has been disclosed. Inhibitory activity on the methyl mercaptan production by oral bacteria of a plant belonging to the family Asteraceae , genera Chrysanthemum, Cynara , and Tagetes (e.g.
- Patent Document 4 the family Rutaceae , genus Zanthoxylum (e.g. Patent Document 5) and a plant essential oil component (e.g. Non-Patent Document 1); and furthermore inhibitory activity on the methyl mercaptan production by oral bacteria of a certain perfume component (e.g. Patent Documents 6 and 7), and ⁇ -ketobutyrate and a salt thereof (e.g. Patent Document 8) have been reported. However, none of them are satisfactory in terms of sustainability of the activity and the like.
- myrsinoic acid A, myrsinoic acid B, myrsinoic acid C, myrsinoic acid E and myrsinoic acid F have been identified from Myrsine seguinii or Rapanaea neriifolia , a plant belonging to the family Myrsinaceae , genus Myrsine , which are substances inhibiting cutaneous inflammation (e.g. Non-Patent Documents 2, 3 and 4).
- myrsinoic acid A is known to inhibit cutaneous inflammation by inhibiting a DNA polymerase, but its odor eliminating activity has not been disclosed.
- Methyl esters of myrsinoic acids A, B and C have been identified from Rapanea unbellata , a plant of the same genus, but the bioactivities have not been disclosed (e.g. Non-Patent Documents 5 and 6).
- Patent Document 1 Japanese Patent Application Laid-Open No. 2002-3353
- Patent Document 2 Japanese Patent Application Laid-Open No. 2003-160459
- Patent Document 3 Japanese Patent Application Laid-Open No. 2005-162697
- Patent Document 4 Japanese Patent Application Laid-Open No. 2002-114660
- Patent Document 5 Japanese Patent Application Laid-Open No. 2003-26527
- Patent Document 6 Japanese Patent Application Laid-Open No. 2001-348308
- Patent Document 7 Japanese Patent Application Laid-Open No. 2002-3369
- Patent Document 8 Japanese Patent Application Laid-Open No. H07-138139
- Non-Patent Document 1 Tsuneda, F., Journal of Odor Research and Engineering, 2000, Vol. 31 (2), p. 91-96
- Non-Patent Document 2 Biosci. Biotechnol. Biochem., 63(9), 1650-1653, 1999
- Non-Patent Document 3 Biosci. Biotechnol. Biochem., 66(3), 655-659, 2002
- Non-Patent Document 4 Biosci. Biotechnol. Biochem., 67(9), 2038-2041, 2003
- Non-Patent Document 5 Biochimicaet Biophysica Acta, 2000 Jun. 1; 1475 (1): 1-4
- Non-Patent Document 6 Phytochemistry, 1991; 30 (6): 2019-2023
- An object of the present invention is to provide a methioninase inhibitor, as well as a composition and a food or drink containing the same, which includes a plant extract that has no adverse effect on human bodies and is highly safe, or myrsinoic acids originated from the plant extract, as an active ingredient, and inhibits methioninase originated from bacteria to suppress the production of an odor substance of methyl mercaptan.
- the present inventors have directed attention to natural extracts, such as galenicals or herbs, that have been used since ancient times to establish time-tested safety, and carried out inhibition tests using a cell lysate and a live cell suspension originated from Fusobacterium nucleatum that is a causative bacterium of bad breath having high methioninase activity, and discovered that an extract obtained from a plant of family Myrsinaceae , genus Myrsine has an inhibitory activity against methioninase and that the active ingredient thereof is myrsinoic acids, thereby completing the present invention.
- natural extracts such as galenicals or herbs
- the present invention provides a methioninase inhibitor, a composition and a food or drink characterized by containing an extract obtained from a plant belonging to the family Myrsinaceae , genus Myrsine , preferably Myrsine seguinii belonging to the family Myrsinaceae , genus Myrsine , as an active ingredient; a methioninase inhibitor, a composition and a food or drink characterized by containing one or more selected from the group consisting of myrsinoic acid A, myrsinoic acid B, myrsinoic acid C, myrsinoic acid E and myrsinoic acid F as an active ingredient; and the methioninase inhibitor, the composition and the food or drink characterized in that the myrsinoic acid A, myrsinoic acid B, myrsinoic acid C, myrsinoic acid E and myrsinoic acid F are obtained from a plant
- the methioninase inhibitor of the present invention has activity to suppress the production of methyl mercaptan, ammonia and ⁇ -ketobutyrate. Consequently, by ingesting orally a food or drink containing the same, or using the same in an oral composition, such as a mouth freshener or a tooth paste, reduction of bad breath, reduction of feces odor and improvement of mouth's and body's internal environment are possible. Additionally the same can be used as an odor inhibitor for the living environment, as for kitchen garbage.
- myrsinoic acid A, myrsinoic acid B, myrsinoic acid C, myrsinoic acid E and myrsinoic acid F (the respective chemical formulas (I) to (V) being shown below) to be used in the present invention can be obtained by synthesis, but are preferably obtained from an extract of the whole, or a leaf, flower, twig, root or fruit part of a plant belonging to the family Myrsinaceae , genus Myrsine .
- Examples of the plant belonging to the family Myrsinaceae include genus Myrsine containing the myrsinoic acids, taimin - tachibana that is a shrub native in middle to southern Japan and a tropical zone.
- Taimin - tachibana also referred to as hichinoki or sogeki has scientific names Myrsine seguinii and Rapanaea neriifolia . Since the fruit of Myrsine seguinii has been traditionally eaten, there is no concern about its safety. As especially preferable parts for extraction, leaf and fruit may be named, because their production masses are large and the extraction rates can be high.
- Myrsinoic acids have very high inhibitory activity, and among them myrsinoic acid B can be exemplified as an especially preferable type due to its strong methioninase inhibitory activity and a high content in the extract.
- the plant is generally first crushed by an appropriate means. Then extraction is conducted according to a conventional extraction method using a mixed solvent of one or more of water, lower alcohols, such as methanol, ethanol, n-propanol, and n-butanol, organic solvents, such as ether, chloroform, ethyl acetate, acetone, glycerin and propylene glycol.
- organic solvents such as ether, chloroform, ethyl acetate, acetone, glycerin and propylene glycol.
- the present invention is directed to a medicament, an oral composition and a food or drink, as an extraction solvent a combination of water and
- the thus obtained extract may be filtered, concentrated under a reduced pressure or freeze-dried before use.
- the extract may be separated and purified to obtain myrsinoic acids by a publicly known means for separation and purification, such as adsorption chromatography, partition chromatography, high performance liquid chromatography and thin-layer chromatography. More specifically, the extract is subjected to a liquid-liquid extraction, to silica gel column chromatography with an ethyl acetate/hexane mixed solvent, and further to octadecyl silylated column chromatography with a methanol/water (85%) mixed solvent containing additionally 0.1% acetic acid to obtain high purity myrsinoic acids by elution, fraction, concentration and drying.
- a publicly known means for separation and purification such as adsorption chromatography, partition chromatography, high performance liquid chromatography and thin-layer chromatography. More specifically, the extract is subjected to a liquid-liquid extraction, to silica
- the methioninase inhibitor of the present invention can be prepared by using as an active ingredient the myrsinoic acids and an extract of a plant belonging to the family Myrsinaceae , genus Myrsine , for example an extract of Myrsine seguinii , prepared as above.
- the ingredient may be dissolved or dispersed in an appropriate liquid vehicle, or mixed with an appropriate powder carrier or adsorbed thereon, and according to need mixed additionally with an emulsifier, a stabilizer or a dispersant to be formulated to a tablet, a powder, an emulsion, a water-dispersible powder, etc.
- the content of the dry extract with respect to the total formulation is preferably 0.001 to 50% by weight, and more preferably 0.01 to 25% by weight.
- the methioninase inhibitor of the present invention is superior in fragrance, taste and safety, it can be mixed and consumed routinely in an oral composition, such as a tooth paste, a mouthwash and a deodorant spray, confections, such as a chewing gum, a candy, a tablet candy, a gummy jelly, a chocolate and a biscuit, frozen desserts, such as an ice cream, a sherbet and a water ice, foods or drinks, such as beverages, soup and jam.
- the content of the dry extract in the food or drink, or composition is about 0.001% by weight or more, and preferably about 0.01% by weight or more. In case of the food or drink, it is favorable to add the same to the content of about 0.001 to 5% by weight, and preferably about 0.01 to 1% by weight considering the taste.
- This test was conducted to prepare a myrsinoic acid, an extract of Myrsine seguinii and comparative various plant extracts.
- Myrsine seguinii and for comparison a whole grass dry powder of Iceland moss, an alkanet root, a guava leaf, and green tea were used.
- a 1% aqueous solution of the extract of a leaf of Myrsine seguinii by 100% ethanol was solvent-fractionated three times with the same volume of hexane, and the solvent was removed by distillation to obtain the hexane fractions (yield about 20%).
- the hexane fractions were subjected to silica gel column chromatography using a mixed solvent of ethyl acetate/hexane (10 to 20%) to separate about 20% of non-polar components to obtain a fraction containing myrsinoic acids (yield about 40%).
- This fraction was subjected to octadecyl silylated column chromatography using 0.1% acetic acid added methanol/water (85%) for elution and separation to obtain 400 mg of myrsinoic acid B and 100 mg of myrsinoic acid C.
- the hexane fractions were subjected to silica gel column chromatography and octadecyl silylated column chromatography for fractionation to obtain 60 mg of myrsinoic acid A, 3 mg of myrsinoic acid E and 1.5 mg of myrsinoic acid F.
- Myrsinoic acid E was obtained by hydrolysis of a methyl ester prepared from 2-iodophenol by geranylation and carbonylation according to the method of Proceedings of Symposium on the Chemistry of Terpenes, Essential Oils, and Aromatics, Vol. 46, 396-398 (2002).
- an alkanet root, a guava leaf and green tea were extracted respectively by 50% ethanol, and the extract liquids were concentrated or freeze-dried to prepare the extracts.
- This test was conducted to examine the methioninase inhibitory activity of myrsinoic acids and the Myrsine seguinii extract.
- the Myrsine seguinii extract and myrsinoic acids as well as for comparison extracts of the whole grass dry powder of Iceland moss, the alkanet root, the guava leaf, and the green tea prepared in Test Example 1 were used.
- Methyl mercaptan, ammonia and ⁇ -ketobutyrate are produced from methionine by a reaction according to the methioninase.
- the production amount of ⁇ -ketobutyrate that is a considerably stable compound among the reaction products was used as an index of the enzyme reaction.
- Fusobacterium nucleatum JCM 8532 was cultured for 2 days under an anaerobic condition and disrupted by sonication to obtain the enzyme (final concentration: 300 ⁇ g-protein/mL), which was then mixed with methionine (final concentration: 30 mM), pyridoxal phosphate (final concentration: 50 ⁇ M), and the sample (final concentration: 200 ⁇ g/mL) in a phosphate buffer solution (50 mM, pH 7.6). After incubation at 37° C.
- the absorbance was corrected using a blank sample (a reaction liquid of the aforedescribed reaction except that methionine is excluded), which value was deducted for compensation.
- the amount of ⁇ -ketobutyrate was determined using a calibration curve prepared in advance, and an inhibition rate was calculated according to the following formula:
- Inhibition rate(%) (( C ⁇ S )/ C ) ⁇ 100
- C is the amount of ⁇ -ketobutyrate in the control
- S is the amount of ⁇ -ketobutyrate when the sample is added.
- the measurement results of the methioninase inhibitory activity are shown in Table 2.
- the extract of the whole grass dry powder of Iceland moss, the extract of the alkanet root, the extract of the guava leaf, and the extract of the green tea see e.g. Japanese Patent Application Laid-Open No. 2003-26527
- whose inhibitory activities against methioninase originated from oral bacterium Fusobacterium nucleatum
- the respective inhibition rates were 29% for the extract of the whole grass dry powder of Iceland moss, 28% for the extract of the alkanet root, 22% for the extract of the guava leaf, and 26% for the extract of the green tea.
- the extracts of a leaf, a twig and a fruit of Myrsine seguinii of the present invention exhibited respectively high inhibitory activities against methioninase.
- the test results showed that the extracts of a leaf, a twig and a fruit of Myrsine seguinii of the present invention have strong inhibitory activities against methioninase.
- myrsinoic acids A, B, C, E, and F inhibited methioninase by 50% at a concentration of 5 ⁇ g/mL or less. Consequently an isolated myrsinoic acid has strong inhibitory activity, indicating that the same is the active ingredient contained in the Myrsine seguinii extract.
- This test was conducted to examine the inhibitory activity against methioninase of living bacteria by myrsinoic acids and the Myrsine seguinii extract.
- the Myrsine seguinii extract as well as for comparison extracts of the whole grass dry powder of Iceland moss, the alkanet root, the guava leaf, and the green tea prepared in Test Example 1 were used.
- Fusobacterium nucleatum JCM8532 cultured for about 16 hours under an anaerobic condition was centrifuged and suspended in a physiological saline buffer solution (40 mM phosphate buffer/50 mM sodium chloride, pH 7.7) to obtain a living bacterial suspension (10% of a reaction system), which was then reacted with methionine (final concentration: 1 mM) and the sample (final concentration: 1 to 200 ⁇ g/mL) in a physiological saline buffer solution at 37° C. for 90 min. Then 0.5 mL of the head-space gas was analyzed by gas chromatography.
- a physiological saline buffer solution 40 mM phosphate buffer/50 mM sodium chloride, pH 7.7
- the results are shown in Table 4.
- the green tea extract was evaluated as a comparative sample, which inhibition rate was 66%.
- the extracts of a Myrsine seguinii leaf of the present invention exhibited a high inhibitory activity against methioninase at a lower concentration.
- compositions such as a tooth paste, a mouthwash, a deodorant spray, a breath spray, a tablet and a powder; and confections, such as a chewing gum, a candy, a tablet candy, a gummy jelly, a chocolate and a biscuit, frozen desserts, such as an ice cream, a sherbet and a water ice, foods or drinks, such as beverage, soup and jam were produced.
- the mixture was filled in an aerosol container together with a propellant gas (nitrogen gas) to prepare a deodorant spray.
- a propellant gas nitrogen gas
- Powder sugar (39.8% by weight)
- the mixture was filled in an aerosol container together with a propellant gas (nitrogen gas) to prepare a deodorant spray.
- a propellant gas nitrogen gas
- the components were finely milled, mixed and formed into tablets by a direct tableting method.
- the total weight of each tablet is 100 mg and the active ingredient therein is 10 mg.
- the components were finely milled and mixed to form powder.
- One hundred (100) mg of the powder was filled in a hard capsule to produce a capsule formulation.
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Abstract
The invention provides a methioninase inhibitor to suppress the production of methyl mercaptan that is a causative substance of a bad smell by inhibiting methioninase originated from bacteria, as well as a composition and a food or drink containing the same, wherein the methioninase inhibitor contains an extract obtained from a plant of the family Myrsinaceae, genus Myrsine, preferably Myrsine seguinii as an active ingredient; and further provides a methioninase inhibitor, as well as a composition and a food or drink containing the same, wherein the methioninase inhibitor contains as an active ingredient one or more selected from the group consisting of myrsinoic acid A, myrsinoic acid B, myrsinoic acid C, myrsinoic acid E and myrsinoic acid F; preferably the myrsinoic acid A, myrsinoic acid B, myrsinoic acid C, myrsinoic acid E and myrsinoic acid F are obtained from a plant of the family Myrsinaceae, genus Myrsine, preferably Myrsine seguinii.
Description
- The present invention relates to an inhibitor of methioninase engaged in production of methyl mercaptan that is a causative substance of a bad smell, as well as a composition and a food or drink containing the same.
- The main components of a bad smell from an oral cavity are volatile sulfur compounds, and especially methyl mercaptan is known to have a high correlation with the intensity of bad breath. Methyl mercaptan is generated from food debris, intraoral desquamated epithelial cells and salivary proteins metabolized or decomposed by oral bacteria. Volatile sulfur compounds such as methyl mercaptan are known to have adverse effects, such as inhibition of intraoral synthesis of proteins, inhibition of collagen synthesis, inhibition of growth and division of endothelial cells, and increase of permeability of intraoral mucosa. Consequently inhibition of methyl mercaptan is an important task not only for suppression of the bad breath, but also for prevention of a periodontal disease or maintenance of the human body's internal environment.
- Methyl mercaptan is generated from L-methionine, that is an intraoral protein decomposition product, utilized as a substrate, by an oral bacterial enzyme of methioninase (L-methionine-γ-lyase). Among various bacteria present in the human oral cavity, Fusobacterium nucleatum and Porphyromonas gingivalis have high methioninase activity, and by inhibiting their methioninase activity the generation of methyl mercaptan, a causative substance of bad smell, as well as hydrogen sulfide, ammonia and α-ketobutyrate can be inhibited. Methyl mercaptan is generated also by enteric bacteria, and known as a causative substance of odors of kitchen garbage and feces. Consequently, by inhibiting methioninase, the odors of garbage and feces are expected to be eliminated.
- For suppressing the bad breath, there are various methods, such as disinfection of the oral bacteria generating the odor, chemical conversion of the odor substance to an odorless substance, and masking of the odor by a perfume. However, the use of a disinfectant might have an adverse effect by disturbing a balance of an intraoral bacterial flora, and the odor eliminating by chemical conversion or masking does not inhibit generation of the odor itself and the effect does not last long. Meanwhile the methioninase inhibitor of the present invention is safe, inhibits the generation of methyl mercaptan itself and therefore shows high sustainability.
- Methioninase inhibitors originated from natural materials, such as a tomato extract and a ginger extract (e.g. Patent Documents 1 and 2), or an extract of Iceland moss, an extract of alkanet, an extract of green tea and the like (e.g. Patent Document 3) have been reported, and their inhibition of the methyl mercaptan production by oral bacteria, such as Porphyromonas gingivalis and Fusobacterium nucleatum, has been disclosed. Inhibitory activity on the methyl mercaptan production by oral bacteria of a plant belonging to the family Asteraceae, genera Chrysanthemum, Cynara, and Tagetes (e.g. Patent Document 4), the family Rutaceae, genus Zanthoxylum (e.g. Patent Document 5) and a plant essential oil component (e.g. Non-Patent Document 1); and furthermore inhibitory activity on the methyl mercaptan production by oral bacteria of a certain perfume component (e.g. Patent Documents 6 and 7), and α-ketobutyrate and a salt thereof (e.g. Patent Document 8) have been reported. However, none of them are satisfactory in terms of sustainability of the activity and the like.
- Meanwhile, myrsinoic acid A, myrsinoic acid B, myrsinoic acid C, myrsinoic acid E and myrsinoic acid F have been identified from Myrsine seguinii or Rapanaea neriifolia, a plant belonging to the family Myrsinaceae, genus Myrsine, which are substances inhibiting cutaneous inflammation (e.g. Non-Patent Documents 2, 3 and 4).
- Furthermore, myrsinoic acid A is known to inhibit cutaneous inflammation by inhibiting a DNA polymerase, but its odor eliminating activity has not been disclosed. Methyl esters of myrsinoic acids A, B and C have been identified from Rapanea unbellata, a plant of the same genus, but the bioactivities have not been disclosed (e.g. Non-Patent Documents 5 and 6).
- Patent Document 1: Japanese Patent Application Laid-Open No. 2002-3353
- Patent Document 2: Japanese Patent Application Laid-Open No. 2003-160459
- Patent Document 3: Japanese Patent Application Laid-Open No. 2005-162697
- Patent Document 4: Japanese Patent Application Laid-Open No. 2002-114660
- Patent Document 5: Japanese Patent Application Laid-Open No. 2003-26527
- Patent Document 6: Japanese Patent Application Laid-Open No. 2001-348308
- Patent Document 7: Japanese Patent Application Laid-Open No. 2002-3369
- Patent Document 8: Japanese Patent Application Laid-Open No. H07-138139
- Non-Patent Document 1: Tsuneda, F., Journal of Odor Research and Engineering, 2000, Vol. 31 (2), p. 91-96
- Non-Patent Document 2: Biosci. Biotechnol. Biochem., 63(9), 1650-1653, 1999
- Non-Patent Document 3: Biosci. Biotechnol. Biochem., 66(3), 655-659, 2002
- Non-Patent Document 4: Biosci. Biotechnol. Biochem., 67(9), 2038-2041, 2003
- Non-Patent Document 5: Biochimicaet Biophysica Acta, 2000 Jun. 1; 1475 (1): 1-4
- Non-Patent Document 6: Phytochemistry, 1991; 30 (6): 2019-2023
- An object of the present invention is to provide a methioninase inhibitor, as well as a composition and a food or drink containing the same, which includes a plant extract that has no adverse effect on human bodies and is highly safe, or myrsinoic acids originated from the plant extract, as an active ingredient, and inhibits methioninase originated from bacteria to suppress the production of an odor substance of methyl mercaptan.
- To solve the problems the present inventors have directed attention to natural extracts, such as galenicals or herbs, that have been used since ancient times to establish time-tested safety, and carried out inhibition tests using a cell lysate and a live cell suspension originated from Fusobacterium nucleatum that is a causative bacterium of bad breath having high methioninase activity, and discovered that an extract obtained from a plant of family Myrsinaceae, genus Myrsine has an inhibitory activity against methioninase and that the active ingredient thereof is myrsinoic acids, thereby completing the present invention.
- More particularly, the present invention provides a methioninase inhibitor, a composition and a food or drink characterized by containing an extract obtained from a plant belonging to the family Myrsinaceae, genus Myrsine, preferably Myrsine seguinii belonging to the family Myrsinaceae, genus Myrsine, as an active ingredient; a methioninase inhibitor, a composition and a food or drink characterized by containing one or more selected from the group consisting of myrsinoic acid A, myrsinoic acid B, myrsinoic acid C, myrsinoic acid E and myrsinoic acid F as an active ingredient; and the methioninase inhibitor, the composition and the food or drink characterized in that the myrsinoic acid A, myrsinoic acid B, myrsinoic acid C, myrsinoic acid E and myrsinoic acid F are obtained from a plant belonging to the family Myrsinaceae, genus Myrsine, preferably Myrsine seguinii belonging to the family Myrsinaceae, genus Myrsine.
- The methioninase inhibitor of the present invention has activity to suppress the production of methyl mercaptan, ammonia and α-ketobutyrate. Consequently, by ingesting orally a food or drink containing the same, or using the same in an oral composition, such as a mouth freshener or a tooth paste, reduction of bad breath, reduction of feces odor and improvement of mouth's and body's internal environment are possible. Additionally the same can be used as an odor inhibitor for the living environment, as for kitchen garbage.
- The present invention will be described in more detail below. Thereby the methioninase inhibitor of the present invention, a composition and a food or drink containing the same as an active ingredient, a process for producing them, and advantages thereof will be described, provided that the present invention be not limited thereto.
- Although myrsinoic acid A, myrsinoic acid B, myrsinoic acid C, myrsinoic acid E and myrsinoic acid F (the respective chemical formulas (I) to (V) being shown below) to be used in the present invention can be obtained by synthesis, but are preferably obtained from an extract of the whole, or a leaf, flower, twig, root or fruit part of a plant belonging to the family Myrsinaceae, genus Myrsine. Examples of the plant belonging to the family Myrsinaceae include genus Myrsine containing the myrsinoic acids, taimin-tachibana that is a shrub native in middle to southern Japan and a tropical zone. Taimin-tachibana also referred to as hichinoki or sogeki has scientific names Myrsine seguinii and Rapanaea neriifolia. Since the fruit of Myrsine seguinii has been traditionally eaten, there is no concern about its safety. As especially preferable parts for extraction, leaf and fruit may be named, because their production masses are large and the extraction rates can be high.
- Myrsinoic acids have very high inhibitory activity, and among them myrsinoic acid B can be exemplified as an especially preferable type due to its strong methioninase inhibitory activity and a high content in the extract.
- Although there is no particular restriction on a preparation method for the plant extraction according to the present invention and a conventionally known method can be applicable, the plant is generally first crushed by an appropriate means. Then extraction is conducted according to a conventional extraction method using a mixed solvent of one or more of water, lower alcohols, such as methanol, ethanol, n-propanol, and n-butanol, organic solvents, such as ether, chloroform, ethyl acetate, acetone, glycerin and propylene glycol. However, since the present invention is directed to a medicament, an oral composition and a food or drink, as an extraction solvent a combination of water and ethanol is preferable in view of the safety. Although extraction any of at a higher temperature, at room temperature and a lower temperature is possible, preferable extraction conditions are approximately at 50 to 80° C. and for 1 to 5 hours.
- The thus obtained extract may be filtered, concentrated under a reduced pressure or freeze-dried before use. The extract may be separated and purified to obtain myrsinoic acids by a publicly known means for separation and purification, such as adsorption chromatography, partition chromatography, high performance liquid chromatography and thin-layer chromatography. More specifically, the extract is subjected to a liquid-liquid extraction, to silica gel column chromatography with an ethyl acetate/hexane mixed solvent, and further to octadecyl silylated column chromatography with a methanol/water (85%) mixed solvent containing additionally 0.1% acetic acid to obtain high purity myrsinoic acids by elution, fraction, concentration and drying.
- The methioninase inhibitor of the present invention can be prepared by using as an active ingredient the myrsinoic acids and an extract of a plant belonging to the family Myrsinaceae, genus Myrsine, for example an extract of Myrsine seguinii, prepared as above. As necessary the ingredient may be dissolved or dispersed in an appropriate liquid vehicle, or mixed with an appropriate powder carrier or adsorbed thereon, and according to need mixed additionally with an emulsifier, a stabilizer or a dispersant to be formulated to a tablet, a powder, an emulsion, a water-dispersible powder, etc. The content of the dry extract with respect to the total formulation is preferably 0.001 to 50% by weight, and more preferably 0.01 to 25% by weight.
- Since the methioninase inhibitor of the present invention is superior in fragrance, taste and safety, it can be mixed and consumed routinely in an oral composition, such as a tooth paste, a mouthwash and a deodorant spray, confections, such as a chewing gum, a candy, a tablet candy, a gummy jelly, a chocolate and a biscuit, frozen desserts, such as an ice cream, a sherbet and a water ice, foods or drinks, such as beverages, soup and jam. The content of the dry extract in the food or drink, or composition is about 0.001% by weight or more, and preferably about 0.01% by weight or more. In case of the food or drink, it is favorable to add the same to the content of about 0.001 to 5% by weight, and preferably about 0.01 to 1% by weight considering the taste.
- The product of the present invention will be described in more detail by means of test examples, provided that they should not be interpreted in any restrictive way concerning the scope of the product of the present invention.
- This test was conducted to prepare a myrsinoic acid, an extract of Myrsine seguinii and comparative various plant extracts.
- 1) Test Samples
- Myrsine seguinii, and for comparison a whole grass dry powder of Iceland moss, an alkanet root, a guava leaf, and green tea were used.
- 2) Test Method
- Each of plant extracts and myrsinoic acids were prepared as described below, provided that the present invention be not limited thereto.
- To 5 g of a dry powder leaf of Myrsine seguinii, 50 mL of water was added for extraction at 70° C. for 2 hours. The obtained extract liquid was filtered and freeze-dried to obtain 0.75 g of an extract.
- Similarly a twig and a fruit of Myrsine seguinii were extracted respectively by water, and the extract liquids were concentrated or freeze-dried to prepare the extracts. The yields of the extracts are shown in Table 1.
- To 5 g of a dry powder twig of Myrsine seguinii, 50 mL of 25% ethanol was added for extraction at 70° C. for 2 hours. The obtained extract liquid was filtered, the solvent was removed, and the residue was freeze-dried to obtain 0.80 g of an extract.
- Similarly a leaf and a fruit of Myrsine seguinii were extracted respectively by 25% ethanol, and the extract liquids were concentrated or freeze-dried to prepare the extracts. The yields of the extracts are shown in Table 1.
- To 5 g of a dry powder fruit of Myrsine seguinii, 50 mL of 50% ethanol was added for extraction at 70° C. for 2 hours. The obtained extract liquid was filtered, the solvent was removed, and the residue was freeze-dried to obtain 0.80 g of an extract.
- Similarly a leaf and a twig of Myrsine seguinii were extracted respectively by 50% ethanol, and the extract liquids were concentrated or freeze-dried to prepare the extracts. The yields of the extracts are shown in Table 1.
- To 5 g of a dry powder leaf of Myrsine seguinii, 50 mL of 75% ethanol was added for extraction at 70° C. for 2 hours. The obtained extract liquid was filtered, the solvent was removed, and the residue was freeze-dried to obtain 0.80 g of an extract.
- Similarly a twig and a fruit of Myrsine seguinii were extracted respectively by 75% ethanol, and the extract liquids were concentrated or freeze-dried to prepare the extracts. The yields of the extracts are shown in Table 1.
- To 5 g of a dry powder leaf of Myrsine seguinii, 50 mL of 100% ethanol was added for extraction at 70° C. for 2 hours. The obtained extract liquid was filtered, and freeze-dried to obtain 0.45 g of an extract.
- Similarly a twig and a fruit of Myrsine seguinii were extracted respectively by 100% ethanol, and the extract liquids were concentrated or freeze-dried to prepare the extracts. The yields of the extracts are shown in Table 1.
- To 5 g of a dry powder fruit of Myrsine seguinii, 50 mL of acetone was added for extraction at 70° C. for 2 hours. The obtained extract liquid was filtered, the solvent was removed, and the residue was freeze-dried to obtain 0.40 g of an extract.
- Similarly a leaf and a twig of Myrsine seguinii were extracted respectively by acetone, and the extract liquids were concentrated or freeze-dried to prepare the extracts. The yields of the extracts are shown in Table 1.
- A 1% aqueous solution of the extract of a leaf of Myrsine seguinii by 100% ethanol was solvent-fractionated three times with the same volume of hexane, and the solvent was removed by distillation to obtain the hexane fractions (yield about 20%). The hexane fractions were subjected to silica gel column chromatography using a mixed solvent of ethyl acetate/hexane (10 to 20%) to separate about 20% of non-polar components to obtain a fraction containing myrsinoic acids (yield about 40%). This fraction was subjected to octadecyl silylated column chromatography using 0.1% acetic acid added methanol/water (85%) for elution and separation to obtain 400 mg of myrsinoic acid B and 100 mg of myrsinoic acid C.
- Similarly, the hexane fractions were subjected to silica gel column chromatography and octadecyl silylated column chromatography for fractionation to obtain 60 mg of myrsinoic acid A, 3 mg of myrsinoic acid E and 1.5 mg of myrsinoic acid F.
- Myrsinoic acid E was obtained by hydrolysis of a methyl ester prepared from 2-iodophenol by geranylation and carbonylation according to the method of Proceedings of Symposium on the Chemistry of Terpenes, Essential Oils, and Aromatics, Vol. 46, 396-398 (2002).
- To 5 g of a dry powder whole grass of Iceland moss, 50 mL of 50% ethanol was added for extraction at 70° C. for 2 hours. The obtained extract liquid was filtered, the solvent was removed, and the residue was freeze-dried to obtain 1.10 g of an extract.
- Similarly an alkanet root, a guava leaf and green tea were extracted respectively by 50% ethanol, and the extract liquids were concentrated or freeze-dried to prepare the extracts.
- 3) Test Results
- The yields of the extracts are shown in Table 1.
-
TABLE 1 Extraction examples of products of the present invention and comparative products Extraction Extraction Plant name solvent yield (%) Part Myrsine seguinii 100% Ethanol 9 Leaf 75% Ethanol 16 50% Ethanol 19 25% Ethanol 19 Water 15 100% Ethanol 5 Twig 75% Ethanol 14 50% Ethanol 15 25% Ethanol 16 Water 13 100% Ethanol 10 Fruit 75% Ethanol 15 50% Ethanol 16 25% Ethanol 16 Water 13 Acetone 8 Iceland moss 50% Ethanol 22 Whole grass Alkanet 50% Ethanol 6 Root Guava 50% Ethanol 22 Leaf Green tea 50% Ethanol 24 Leaf - This test was conducted to examine the methioninase inhibitory activity of myrsinoic acids and the Myrsine seguinii extract.
- 1) Test Samples
- The Myrsine seguinii extract and myrsinoic acids, as well as for comparison extracts of the whole grass dry powder of Iceland moss, the alkanet root, the guava leaf, and the green tea prepared in Test Example 1 were used.
- 2) Test Method (Cell-free Methioninase Inhibition Test)
- Methyl mercaptan, ammonia and α-ketobutyrate are produced from methionine by a reaction according to the methioninase. The production amount of α-ketobutyrate that is a considerably stable compound among the reaction products was used as an index of the enzyme reaction.
- More specifically, Fusobacterium nucleatum JCM 8532 was cultured for 2 days under an anaerobic condition and disrupted by sonication to obtain the enzyme (final concentration: 300 μg-protein/mL), which was then mixed with methionine (final concentration: 30 mM), pyridoxal phosphate (final concentration: 50 μM), and the sample (final concentration: 200 μg/mL) in a phosphate buffer solution (50 mM, pH 7.6). After incubation at 37° C. for 1 hour, 1 mL of the reaction liquid was mixed with a ½ volume of an aqueous solution of perchloric acid (6%) to degenerate the proteins and the mixture was centrifuged at 3000×g for 10 min for separating the precipitates to obtain the sample liquid. In order to quantify the reaction byproduct of α-ketobutyrate, to 0.4 mL of the sample liquid were added 0.4 mL of a 0.05% 3-methyl-2-benzothiazolinonehydrazone (MBTH) solution and 0.8 mL of a 1 M sodium acetate buffer solution (pH 5.0), and the mixture was reacted at 50° C. for 30 min. After the reaction and confirming that the temperature of the reaction liquid was cooled down to room temperature, the absorbance (335 nm) was measured.
- Since the color of the sample to be evaluated may have some influence on the result of the quantification reaction of α-ketobutyrate, the absorbance was corrected using a blank sample (a reaction liquid of the aforedescribed reaction except that methionine is excluded), which value was deducted for compensation.
- The amount of α-ketobutyrate was determined using a calibration curve prepared in advance, and an inhibition rate was calculated according to the following formula:
-
Inhibition rate(%)=((C−S)/C)×100 - wherein C is the amount of α-ketobutyrate in the control, and S is the amount of α-ketobutyrate when the sample is added.
- 3) Test Results
- The measurement results of the methioninase inhibitory activity are shown in Table 2. For comparison, the extract of the whole grass dry powder of Iceland moss, the extract of the alkanet root, the extract of the guava leaf, and the extract of the green tea (see e.g. Japanese Patent Application Laid-Open No. 2003-26527), whose inhibitory activities against methioninase originated from oral bacterium (Fusobacterium nucleatum) have been known, were also evaluated. The respective inhibition rates were 29% for the extract of the whole grass dry powder of Iceland moss, 28% for the extract of the alkanet root, 22% for the extract of the guava leaf, and 26% for the extract of the green tea.
- The extracts of a leaf, a twig and a fruit of Myrsine seguinii of the present invention exhibited respectively high inhibitory activities against methioninase. The test results showed that the extracts of a leaf, a twig and a fruit of Myrsine seguinii of the present invention have strong inhibitory activities against methioninase.
- As shown in Table 3, myrsinoic acids A, B, C, E, and F inhibited methioninase by 50% at a concentration of 5 μg/mL or less. Consequently an isolated myrsinoic acid has strong inhibitory activity, indicating that the same is the active ingredient contained in the Myrsine seguinii extract.
-
TABLE 2 Inhibitory activities against methioninase of products of the present invention and comparative products Extraction Inhibition Plant name solvent rate (%) Part Myrsine seguinii 100% Ethanol 100 Leaf 75% Ethanol 100 50% Ethanol 100 25% Ethanol 100 Water 24 100% Ethanol 100 Twig 75% Ethanol 100 50% Ethanol 100 25% Ethanol 100 Water 78 100% Ethanol 100 Fruit 75% Ethanol 100 50% Ethanol 100 25% Ethanol 100 Water 50 Acetone 100 Iceland moss 50% Ethanol 29 Whole grass Alkanet 50% Ethanol 28 Root Guava 50% Ethanol 22 Leaf Green tea 50% Ethanol 26 Leaf -
TABLE 3 Inhibitory activities (IC50) against methioninase of products of the present invention and comparative products Sample IC50 (μg/ml) Extract of Myrsine seguinii by 30 100% ethanol Myrsinoic acid A 5 Myrsinoic acid B 4 Myrsinoic acid C 5 Myrsinoic acid E 5 Myrsinoic acid F 3 Myrsinoic acid E (Synthesized) 5 Green tea 200 Extract of green tea by 50% ethanol - This test was conducted to examine the inhibitory activity against methioninase of living bacteria by myrsinoic acids and the Myrsine seguinii extract.
- 1) Test Samples
- The Myrsine seguinii extract, as well as for comparison extracts of the whole grass dry powder of Iceland moss, the alkanet root, the guava leaf, and the green tea prepared in Test Example 1 were used.
- 2) Test Method (Inhibition Test against Methioninase of Living Bacteria)
- In order to evaluate an odor eliminating activity under conditions closer to human intraoral conditions, Fusobacterium nucleatum JCM8532 cultured for about 16 hours under an anaerobic condition was centrifuged and suspended in a physiological saline buffer solution (40 mM phosphate buffer/50 mM sodium chloride, pH 7.7) to obtain a living bacterial suspension (10% of a reaction system), which was then reacted with methionine (final concentration: 1 mM) and the sample (final concentration: 1 to 200 μg/mL) in a physiological saline buffer solution at 37° C. for 90 min. Then 0.5 mL of the head-space gas was analyzed by gas chromatography.
- 3) Test Results
- The results are shown in Table 4. The green tea extract was evaluated as a comparative sample, which inhibition rate was 66%. The extracts of a Myrsine seguinii leaf of the present invention exhibited a high inhibitory activity against methioninase at a lower concentration.
-
TABLE 4 Inhibitory activities against methioninase of living bacteria by product of the present invention and comparative product Concentration Inhibition Sample (μg/mL) rate (%) Extract of Myrsine 200 100 seguinii leaf by 100% 100 86 ethanol 50 83 25 55 10 24 1 10 Extract of green tea 200 66 by 50% ethanol - The present invention will now be described in more detail by way of examples thereof, provided that the examples should not be interpreted in any restrictive way concerning the scope of the present invention.
- Using a product of the present invention prepared by a method described in Sample Preparation Examples 1 to 7, compositions, such as a tooth paste, a mouthwash, a deodorant spray, a breath spray, a tablet and a powder; and confections, such as a chewing gum, a candy, a tablet candy, a gummy jelly, a chocolate and a biscuit, frozen desserts, such as an ice cream, a sherbet and a water ice, foods or drinks, such as beverage, soup and jam were produced.
- Formulation of tooth paste
- Calcium carbonate (50.0% by weight)
- Glycerin (20.0)
- Carboxymethylcellulose (2.0)
- Sodium lauryl sulfate (2.0)
- Perfume (1.0)
- Saccharin (0.1)
- Extract of Myrsine seguinii fruit by water in Sample Preparation Example 1 (1.0)
- Chlorhexidine (0.01)
- Water (balance)
- Total (100.0)
- Formulation of mouthwash
- Ethanol (2.0% by weight)
- Perfume (1.0)
- Saccharin (0.05)
- Chlorhexidine hydrochloride (0.01)
- Extract of Myrsine seguinii twig by 25% ethanol in Sample Preparation Example 2 (0.5)
- Water (balance)
- Total (100.0)
- Formulation of deodorant spray
- Ethanol (49.5% by weight)
- Extract of Myrsine seguinii fruit by 50% ethanol in Sample Preparation Example 3 (0.5)
- Water (50.0)
- Total (100.0)
- The mixture was filled in an aerosol container together with a propellant gas (nitrogen gas) to prepare a deodorant spray.
- Formulation of breath spray
- Ethanol (10.0% by weight)
- Glycerin (5.0)
- Extract of Myrsine seguinii leaf by 50% ethanol in Sample Preparation Example 3 (1.0)
- Perfume (0.05)
- Colorant (0.001)
- Water (balance)
- Total (100.0)
- Formulation of lozenge
- Dextrose (72.3% by weight)
- Lactose (19.0)
- Gum arabic (6.0)
- Perfume (1.0)
- Sodium monofluorophosphate (0.7)
- Extract of Myrsine seguinii fruit by 75% ethanol in Sample Preparation Example 4 (1.0)
- Total (100.0)
- Formulation of chewing gum
- Gum base (20.0% by weight)
- Sugar (55.0)
- Glucose (15.0)
- Glutinous starch syrup (9.0)
- Perfume (0.5)
- Extract of Myrsine seguinii leaf by 100% ethanol in Sample Preparation Example 5 (0.5)
- Total (100.0)
- Formulation of candy
- Sugar (50.0% by weight)
- Glutinous starch syrup (34.0)
- Perfume (0.5)
- Extract of Myrsine seguinii fruit by acetone in Sample Preparation Example 6 (0.5)
- Water (balance)
- Total (100.0)
- Formulation of tablet candy
- Sugar (76.4% by weight)
- Glucose (19.0)
- Sucrose fatty acid ester (0.2)
- Perfume (0.2)
- Extract of Myrsine seguinii twig by water in Sample Preparation Example 1 (0.1)
- Water (balance)
- Total (100.0)
- Formulation of gummy jelly
- Gelatin (60.0% by weight)
- Glutinous starch syrup (23.0)
- Sugar (8.5)
- Vegetable fat and oil (4.5)
- Mannitol (2.95)
- Lemon fruit juice (1.0)
- Extract of Myrsine seguinii leaf by 25% ethanol in Sample Preparation Example 2 (0.05)
- Total (100.0)
- Formulation of chocolate
- Powder sugar (39.8% by weight)
- Cacao bitter (20.0)
- Whole milk powder (20.0)
- Cocoa butter (17.0)
- Mannitol (2.0)
- Extract of Myrsine seguinii fruit by 50% ethanol in Sample Preparation Example 3 (1.0)
- Perfume (0.2)
- Total (100.0)
- Formulation of biscuit
- Weak flour class 1 (25.59% by weight)
- All-purpose flour class 1 (22.22)
- Refined sugar (4.8)
- Cooking salt (0.73)
- Dextrose (0.78)
- Palm shortening (11.78)
- Sodium bicarbonate (0.17)
- Sodium bisulfite (0.16)
- Rice flour (1.45)
- Whole milk powder (1.16)
- Milk substitute powder (0.29)
- Extract of Myrsine seguinii twig by 75% ethanol in Sample Preparation Example 4 (0.5)
- Water (balance)
- Total (100.0)
- Formulation of ice cream
- Skim milk powder (50.0% by weight)
- Cream (25.0)
- Sugar (10.0)
- Yolk (10.0)
- Myrsinoic acid B in Sample Preparation Example 7 (0.1)
- Perfume (0.1)
- Water (balance)
- Total (100.0)
- Formulation of sherbet
- Orange fruit juice (25.0% by weight)
- Albumen (10.0)
- Myrsinoic acid A in Sample Preparation Example 7 (0.2)
- Water (balance)
- Total (100.0)
- Formulation of drink
- Orange fruit juice (30.0% by weight)
- Isomerized sugar (15.24)
- Citric acid (0.1)
- Vitamin C (0.04)
- Perfume (0.1)
- Myrsinoic acid C in Sample Preparation Example 7 (0.1)
- Water (balance)
- Total (100.0)
- Formulation of soup
- Milk (60.00% by weight)
- Onion (20.00)
- Carrot (10.00)
- Vegetable stock (1.00)
- Butter (0.10)
- Pepper (0.05)
- Cooking salt (0.05)
- Myrsinoic acid E in Sample Preparation Example 7 (0.01)
- Water (balance)
- Total (100.0)
- Formulation of jam
- Fruit flesh (4.0% by weight)
- Sugar (65.0)
- Clear fruit juice (25.0)
- Citric acid (0.5)
- Myrsinoic acid F in Sample Preparation Example 7 (0.02)
- Water (balance)
- Total (100.0)
- Formulation of tooth paste
- Calcium carbonate (50.0% by weight)
- Glycerin (20.0)
- Carboxymethylcellulose (2.0)
- Sodium lauryl sulfate (2.0)
- Perfume (1.0)
- Saccharin (0.1)
- Myrsinoic acid B in Sample Preparation Example 7 (0.1)
- Myrsinoic acid C in Sample Preparation Example 7 (0.01)
- Chlorhexidine (0.01)
- Water (balance)
- Total (100.0)
- Formulation of deodorant spray
- Ethanol (49.5% by weight)
- Myrsinoic acid B in Sample Preparation Example 7 (0.05)
- Water (50.45)
- Total (100.0)
- The mixture was filled in an aerosol container together with a propellant gas (nitrogen gas) to prepare a deodorant spray.
- Formulation of breath spray
- Ethanol (10.0% by weight)
- Glycerin (5.0)
- Myrsinoic acid B in Sample Preparation Example 7 (0.1)
- Myrsinoic acid E in Sample Preparation Example 7 (0.01)
- Perfume (0.05)
- Colorant (0.001)
- Water (balance)
- Total (100.0)
- Formulation of chewing gum
- Gum base (20.0% by weight)
- Sugar (55.0)
- Glucose (15.0)
- Glutinous starch syrup (9.0)
- Perfume (0.5)
- Myrsinoic acid A in Sample Preparation Example 7 (0.1)
- Myrsinoic acid B in Sample Preparation Example 7 (0.01)
- Total (100.0)
- Formulation of candy
- Sugar (50.0% by weight)
- Glutinous starch syrup (34.0)
- Perfume (0.5)
- Myrsinoic acid B in Sample Preparation Example 7 (0.05)
- Myrsinoic acid F in Sample Preparation Example 7 (0.005)
- Water (balance)
- Total (100.0)
- Formulation of tablet candy
- Sugar (76.4% by weight)
- Glucose (19.0)
- Sucrose fatty acid ester (0.2)
- Perfume (0.2)
- Myrsinoic acid A in Sample Preparation Example 7 (0.01)
- Myrsinoic acid E in Sample Preparation Example 7 (0.001)
- Water (balance)
- Total (100.0)
- Formulation of tablet
- Myrsinoic acid B in Sample Preparation Example 7 (0.5% by weight)
- Lactose (70.0)
- Crystalline cellulose (15.0)
- Magnesium stearate (5.0)
- Total (100.0)
- The components were finely milled, mixed and formed into tablets by a direct tableting method. The total weight of each tablet is 100 mg and the active ingredient therein is 10 mg.
- Formulation of powder
- Myrsinoic acid C in Sample Preparation Example 7 (0.05% by weight)
- Corn starch (59.05)
- Carboxycellulose (40.0)
- Total (100.0)
- The components were finely milled and mixed to form powder. One hundred (100) mg of the powder was filled in a hard capsule to produce a capsule formulation.
- Formulation of chewing gum
- Gum base (19.4% by weight)
- Sugar (55.0)
- Glucose (15.0)
- Glutinous starch syrup (9.0)
- Perfume (0.5)
- Extract of Myrsine seguinii leaf by 75% ethanol in
- Sample Preparation Example 4 (1.0)
- Myrsinoic acid B in Sample Preparation Example 7 (0.1)
- Total (100.0)
- Formulation of breath spray
- Ethanol (10.0% by weight)
- Glycerin (5.0)
- Extract of Myrsine seguinii leaf by water in Sample Preparation Example 1 (1.1)
- Myrsinoic acid B in Sample Preparation Example 7 (0.01)
- Myrsinoic acid C in Sample Preparation Example 7 (0.01)
- Perfume (0.05)
- Colorant (0.001)
- Water (balance)
- Total (100.0)
- Formulation of candy
- Sugar (50.0% by weight)
- Glutinous starch syrup (34.0)
- Perfume (0.5)
- Myrsinoic acid E in Sample Preparation Example 9
- Water (balance)
- Total (100.0)
Claims (15)
1. A methioninase inhibitor comprising an extract of a plant belonging to family Myrsinaceae, genus Myrsine as an active ingredient.
2. The methioninase inhibitor according to claim 1 , wherein the plant belonging to family Myrsinaceae, genus Myrsine is Myrsine seguinii.
4. The methioninase inhibitor according to claim 3 , wherein the myrsinoic acid A, myrsinoic acid B, myrsinoic acid C, myrsinoic acid E and myrsinoic acid F are extracted from a plant belonging to family Myrsinaceae, genus Myrsine.
5. The methioninase inhibitor according to claim 4 , wherein the plant belonging to family Myrsinaceae, genus Myrsine is Myrsine seguinii.
6. A composition comprising the methioninase inhibitor according to claim 1 as an active ingredient.
7. A food or drink comprising the methioninase inhibitor according to claim 1 as an active ingredient.
8. A composition comprising the methioninase inhibitor according to claim 2 as an active ingredient.
9. A composition comprising the methioninase inhibitor according to claim 3 as an active ingredient.
10. A composition comprising the methioninase inhibitor according to claim 4 as an active ingredient.
11. A composition comprising the methioninase inhibitor according to claim 5 as an active ingredient.
12. A food or drink comprising the methioninase inhibitor according to claim 2 as an active ingredient.
13. A food or drink comprising the methioninase inhibitor according to claim 3 as an active ingredient.
14. A food or drink comprising the methioninase inhibitor according to claim 4 as an active ingredient.
15. A food or drink comprising the methioninase inhibitor according to claim 5 as an active ingredient.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2006204494A JP5089100B2 (en) | 2006-07-27 | 2006-07-27 | Methioninase inhibitor, composition containing the same, and food and drink |
JP2006-204494 | 2006-07-27 | ||
PCT/JP2007/063908 WO2008013062A1 (en) | 2006-07-27 | 2007-07-12 | Methioninase inhibitor and composition and food or drink containing the same |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2007/063908 A-371-Of-International WO2008013062A1 (en) | 2006-07-27 | 2007-07-12 | Methioninase inhibitor and composition and food or drink containing the same |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US14/314,851 Division US9144539B2 (en) | 2006-07-27 | 2014-06-25 | Methioninase inhibitor and composition and food or drink containing the same |
Publications (1)
Publication Number | Publication Date |
---|---|
US20100069477A1 true US20100069477A1 (en) | 2010-03-18 |
Family
ID=38981375
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US12/309,645 Abandoned US20100069477A1 (en) | 2006-07-27 | 2007-07-12 | Methioninase inhibitor and composition and food or drink containing the same |
US14/314,851 Expired - Fee Related US9144539B2 (en) | 2006-07-27 | 2014-06-25 | Methioninase inhibitor and composition and food or drink containing the same |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US14/314,851 Expired - Fee Related US9144539B2 (en) | 2006-07-27 | 2014-06-25 | Methioninase inhibitor and composition and food or drink containing the same |
Country Status (6)
Country | Link |
---|---|
US (2) | US20100069477A1 (en) |
JP (1) | JP5089100B2 (en) |
KR (1) | KR101441149B1 (en) |
CN (1) | CN101495127B (en) |
HK (1) | HK1135022A1 (en) |
WO (1) | WO2008013062A1 (en) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011014257A1 (en) * | 2009-07-31 | 2011-02-03 | The General Hospital Corporation | Approaches to treat cancer using hb-egf inhibitors such as myrsinoic acid a |
WO2013019955A1 (en) * | 2011-08-02 | 2013-02-07 | The Procter & Gamble Company | Process for surfactant taste and/or odor improvement |
US9072671B2 (en) | 2012-08-02 | 2015-07-07 | The Procter & Gamble Company | Process for oral care material taste and/or odor improvement |
US9078826B2 (en) | 2011-08-02 | 2015-07-14 | The Procter & Gamble Company | Water-soluble surfactant compositions having improved taste |
US10390555B2 (en) * | 2013-04-25 | 2019-08-27 | Japan Tobacco Inc. | Manufacturing method of composition element of item including flavor component, and composition element of item, including flavor component |
US10413844B2 (en) | 2011-08-02 | 2019-09-17 | The Procter & Gamble Company | Liquid-liquid extraction composition useful in processing water-soluble surfactants |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102009020729A1 (en) | 2009-05-11 | 2010-11-18 | Symrise Gmbh & Co. Kg | Use of new or known benzoic acid compounds e.g. as radical scavengers and/or antioxidant to non-therapeutic purposes, to protect tissues and/or cells from oxidative processes and/or radicals, and in a cosmetic or dermatological preparation |
JP6266891B2 (en) | 2013-03-29 | 2018-01-24 | 株式会社ロッテ | Oral cleaning composition |
JP6165077B2 (en) * | 2014-02-10 | 2017-07-19 | 森永乳業株式会社 | Sulfur-containing amino acid lyase inhibitor |
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US5264238A (en) * | 1990-06-12 | 1993-11-23 | House Food Industrial Co., Ltd. | Method for manufacturing snack foods |
US6391344B2 (en) * | 1999-12-02 | 2002-05-21 | Nagase & Company, Ltd. | Method of promoting synthesis of nerve growth factor |
US6723304B2 (en) * | 2001-11-13 | 2004-04-20 | Noville, Inc. | Oral care compositions comprising diglycerol |
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JPH06183940A (en) * | 1992-12-21 | 1994-07-05 | Lion Corp | Composition for oral cavity |
JP3741914B2 (en) * | 1999-09-30 | 2006-02-01 | ライオン株式会社 | Deodorant composition |
JP2005029571A (en) | 2003-06-16 | 2005-02-03 | Yoshiyuki Mizushina | COMPOUND HAVING DNA SYNTHETASE lambda INHIBITORY ACTION AND USE OF THE SAME |
JP4531494B2 (en) * | 2004-09-01 | 2010-08-25 | 三栄源エフ・エフ・アイ株式会社 | Peptide-containing beverage |
JP2006219389A (en) * | 2005-01-14 | 2006-08-24 | Sanei Gen Ffi Inc | Low-density lipoprotein oxidation inhibitor |
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2006
- 2006-07-27 JP JP2006204494A patent/JP5089100B2/en active Active
-
2007
- 2007-07-12 KR KR1020097003784A patent/KR101441149B1/en active IP Right Grant
- 2007-07-12 CN CN2007800285057A patent/CN101495127B/en not_active Expired - Fee Related
- 2007-07-12 WO PCT/JP2007/063908 patent/WO2008013062A1/en active Application Filing
- 2007-07-12 US US12/309,645 patent/US20100069477A1/en not_active Abandoned
-
2010
- 2010-01-13 HK HK10100376.2A patent/HK1135022A1/en not_active IP Right Cessation
-
2014
- 2014-06-25 US US14/314,851 patent/US9144539B2/en not_active Expired - Fee Related
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US5264238A (en) * | 1990-06-12 | 1993-11-23 | House Food Industrial Co., Ltd. | Method for manufacturing snack foods |
US6391344B2 (en) * | 1999-12-02 | 2002-05-21 | Nagase & Company, Ltd. | Method of promoting synthesis of nerve growth factor |
US6723304B2 (en) * | 2001-11-13 | 2004-04-20 | Noville, Inc. | Oral care compositions comprising diglycerol |
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Dong et al., "3-Geranyl-4-hydroxy-5-(3'-methyl-2'-butenyl)benzoic Acid as an Anti-inflammatory Compound from Myrsine seguinii," Biosci. Biotechnol.Biochem., 63 (9) pgs 1650-1653, 1999. * |
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Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011014257A1 (en) * | 2009-07-31 | 2011-02-03 | The General Hospital Corporation | Approaches to treat cancer using hb-egf inhibitors such as myrsinoic acid a |
US20110060047A1 (en) * | 2009-07-31 | 2011-03-10 | The General Hospital Corporation D/B/A Massachusetts General Hospital | Approaches to treat cancer using hb-egf inhibitors |
WO2013019955A1 (en) * | 2011-08-02 | 2013-02-07 | The Procter & Gamble Company | Process for surfactant taste and/or odor improvement |
US8697036B2 (en) | 2011-08-02 | 2014-04-15 | The Procter & Gamble Company | Process for surfactant taste and/or odor improvement |
US9078826B2 (en) | 2011-08-02 | 2015-07-14 | The Procter & Gamble Company | Water-soluble surfactant compositions having improved taste |
AU2012289995B2 (en) * | 2011-08-02 | 2015-08-27 | The Procter & Gamble Company | Process for surfactant taste and/or odor improvement |
US10413844B2 (en) | 2011-08-02 | 2019-09-17 | The Procter & Gamble Company | Liquid-liquid extraction composition useful in processing water-soluble surfactants |
US10653601B2 (en) | 2011-08-02 | 2020-05-19 | The Procter & Gamble Company | Water soluble surfactant composition having improved taste |
US9072671B2 (en) | 2012-08-02 | 2015-07-07 | The Procter & Gamble Company | Process for oral care material taste and/or odor improvement |
US10390555B2 (en) * | 2013-04-25 | 2019-08-27 | Japan Tobacco Inc. | Manufacturing method of composition element of item including flavor component, and composition element of item, including flavor component |
Also Published As
Publication number | Publication date |
---|---|
CN101495127B (en) | 2012-06-13 |
CN101495127A (en) | 2009-07-29 |
HK1135022A1 (en) | 2010-05-28 |
WO2008013062A1 (en) | 2008-01-31 |
JP5089100B2 (en) | 2012-12-05 |
JP2008031062A (en) | 2008-02-14 |
US9144539B2 (en) | 2015-09-29 |
KR101441149B1 (en) | 2014-09-17 |
US20140308218A1 (en) | 2014-10-16 |
KR20090035605A (en) | 2009-04-09 |
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Owner name: LOTTE CO., LTD.,JAPAN Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:ITOH, SATOMI;NARISE, ATSUSHI;TSUGANE, TAKANORI;AND OTHERS;REEL/FRAME:023597/0600 Effective date: 20091117 |
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