US20090137006A1 - Sugar Kinases with Expanded Substrate Specificity and Their Use - Google Patents
Sugar Kinases with Expanded Substrate Specificity and Their Use Download PDFInfo
- Publication number
- US20090137006A1 US20090137006A1 US12/356,208 US35620809A US2009137006A1 US 20090137006 A1 US20090137006 A1 US 20090137006A1 US 35620809 A US35620809 A US 35620809A US 2009137006 A1 US2009137006 A1 US 2009137006A1
- Authority
- US
- United States
- Prior art keywords
- galactose
- sugar
- galk
- variant
- deoxy
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 235000000346 sugar Nutrition 0.000 title claims abstract description 146
- 239000000758 substrate Substances 0.000 title description 78
- 108091000080 Phosphotransferase Proteins 0.000 title description 30
- 102000020233 phosphotransferase Human genes 0.000 title description 30
- 238000000034 method Methods 0.000 claims abstract description 113
- 241000588724 Escherichia coli Species 0.000 claims abstract description 60
- 229910019142 PO4 Inorganic materials 0.000 claims abstract description 36
- 239000010452 phosphate Substances 0.000 claims abstract description 32
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims abstract description 31
- 150000008163 sugars Chemical class 0.000 claims abstract description 31
- 238000000338 in vitro Methods 0.000 claims abstract description 27
- 102000051366 Glycosyltransferases Human genes 0.000 claims abstract description 22
- 108700023372 Glycosyltransferases Proteins 0.000 claims abstract description 22
- 230000035772 mutation Effects 0.000 claims abstract description 18
- 101100174763 Mus musculus Galk1 gene Proteins 0.000 claims abstract description 17
- 239000002773 nucleotide Substances 0.000 claims abstract description 15
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 15
- 239000001226 triphosphate Substances 0.000 claims abstract description 15
- 235000011178 triphosphate Nutrition 0.000 claims abstract description 15
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 claims abstract description 15
- -1 R28 Chemical class 0.000 claims abstract description 14
- 150000001413 amino acids Chemical class 0.000 claims abstract description 13
- 230000000865 phosphorylative effect Effects 0.000 claims abstract description 5
- WQZGKKKJIJFFOK-SVZMEOIVSA-N (+)-Galactose Chemical group OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-SVZMEOIVSA-N 0.000 claims description 58
- 210000004027 cell Anatomy 0.000 claims description 36
- GZCGUPFRVQAUEE-AZGQCCRYSA-N aldehydo-L-altrose Chemical compound OC[C@H](O)[C@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-AZGQCCRYSA-N 0.000 claims description 27
- IAJILQKETJEXLJ-RSJOWCBRSA-N aldehydo-D-galacturonic acid Chemical compound O=C[C@H](O)[C@@H](O)[C@@H](O)[C@H](O)C(O)=O IAJILQKETJEXLJ-RSJOWCBRSA-N 0.000 claims description 22
- AEMOLEFTQBMNLQ-UHFFFAOYSA-N beta-D-galactopyranuronic acid Natural products OC1OC(C(O)=O)C(O)C(O)C1O AEMOLEFTQBMNLQ-UHFFFAOYSA-N 0.000 claims description 22
- AEMOLEFTQBMNLQ-DTEWXJGMSA-N D-Galacturonic acid Natural products O[C@@H]1O[C@H](C(O)=O)[C@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-DTEWXJGMSA-N 0.000 claims description 21
- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 claims description 21
- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 claims description 20
- SHZGCJCMOBCMKK-SVZMEOIVSA-N D-fucopyranose Chemical compound C[C@H]1OC(O)[C@H](O)[C@@H](O)[C@H]1O SHZGCJCMOBCMKK-SVZMEOIVSA-N 0.000 claims description 19
- KDSPLKNONIUZSZ-HSUXUTPPSA-N (2r,4r,5r)-2,4,5,6-tetrahydroxyhexanal Chemical compound OC[C@@H](O)[C@H](O)C[C@@H](O)C=O KDSPLKNONIUZSZ-HSUXUTPPSA-N 0.000 claims description 18
- WQZGKKKJIJFFOK-ZZWDRFIYSA-N L-glucose Chemical compound OC[C@@H]1OC(O)[C@@H](O)[C@H](O)[C@H]1O WQZGKKKJIJFFOK-ZZWDRFIYSA-N 0.000 claims description 18
- GZCGUPFRVQAUEE-KAZBKCHUSA-N aldehydo-D-talose Chemical compound OC[C@@H](O)[C@H](O)[C@H](O)[C@H](O)C=O GZCGUPFRVQAUEE-KAZBKCHUSA-N 0.000 claims description 18
- VQJHQYFOCBRCGA-DHVFOXMCSA-N (2r,3s,4r,5s)-6-amino-2,3,4,5,6-pentahydroxyhexanal Chemical compound NC(O)[C@@H](O)[C@H](O)[C@H](O)[C@@H](O)C=O VQJHQYFOCBRCGA-DHVFOXMCSA-N 0.000 claims description 17
- LCTORNIWLGOBPB-SVZMEOIVSA-N (3r,4s,5r,6r)-2-amino-6-(hydroxymethyl)oxane-2,3,4,5-tetrol Chemical compound NC1(O)O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O LCTORNIWLGOBPB-SVZMEOIVSA-N 0.000 claims description 17
- VRYALKFFQXWPIH-HSUXUTPPSA-N 2-deoxy-D-galactose Chemical compound OC[C@@H](O)[C@H](O)[C@H](O)CC=O VRYALKFFQXWPIH-HSUXUTPPSA-N 0.000 claims description 17
- 108090000623 proteins and genes Proteins 0.000 claims description 17
- 238000006467 substitution reaction Methods 0.000 claims description 10
- 241000894006 Bacteria Species 0.000 claims description 9
- 229930182830 galactose Natural products 0.000 claims description 9
- 241000187398 Streptomyces lividans Species 0.000 claims description 8
- 229940126575 aminoglycoside Drugs 0.000 claims description 8
- 239000001654 beetroot red Substances 0.000 claims description 7
- 102000004169 proteins and genes Human genes 0.000 claims description 7
- 150000001875 compounds Chemical class 0.000 claims description 6
- 239000002207 metabolite Substances 0.000 claims description 6
- 239000002253 acid Substances 0.000 claims description 5
- ZYGHJZDHTFUPRJ-UHFFFAOYSA-N benzo-alpha-pyrone Natural products C1=CC=C2OC(=O)C=CC2=C1 ZYGHJZDHTFUPRJ-UHFFFAOYSA-N 0.000 claims description 5
- 235000001671 coumarin Nutrition 0.000 claims description 5
- VVVPGLRKXQSQSZ-UHFFFAOYSA-N indolo[3,2-c]carbazole Chemical class C1=CC=CC2=NC3=C4C5=CC=CC=C5N=C4C=CC3=C21 VVVPGLRKXQSQSZ-UHFFFAOYSA-N 0.000 claims description 5
- 229960005544 indolocarbazole Drugs 0.000 claims description 5
- 150000004291 polyenes Chemical class 0.000 claims description 5
- 229930001119 polyketide Natural products 0.000 claims description 5
- 125000000830 polyketide group Chemical group 0.000 claims description 5
- 229930001118 polyketide hybrid Natural products 0.000 claims description 5
- 125000003308 polyketide hybrid group Chemical group 0.000 claims description 5
- 230000002194 synthesizing effect Effects 0.000 claims description 5
- 101100165658 Alternaria brassicicola bsc5 gene Proteins 0.000 claims description 4
- 101100032924 Bacillus subtilis (strain 168) radA gene Proteins 0.000 claims description 4
- 108010006654 Bleomycin Proteins 0.000 claims description 4
- 101100371759 Dictyostelium discoideum ugt52 gene Proteins 0.000 claims description 4
- 101100492392 Didymella fabae pksAC gene Proteins 0.000 claims description 4
- YLQBMQCUIZJEEH-UHFFFAOYSA-N Furan Chemical group C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 claims description 4
- 101100226893 Phomopsis amygdali PaP450-2 gene Proteins 0.000 claims description 4
- 229930183506 Pluramycin Natural products 0.000 claims description 4
- 101000928475 Streptomyces galilaeus Aclacinomycin-T 2-deoxy-L-fucose transferase Proteins 0.000 claims description 4
- 150000007513 acids Chemical class 0.000 claims description 4
- 229940045799 anthracyclines and related substance Drugs 0.000 claims description 4
- 230000000975 bioactive effect Effects 0.000 claims description 4
- OYVAGSVQBOHSSS-UAPAGMARSA-O bleomycin A2 Chemical class N([C@H](C(=O)N[C@H](C)[C@@H](O)[C@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCCC=1SC=C(N=1)C=1SC=C(N=1)C(=O)NCCC[S+](C)C)[C@@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@H](CO)O1)O[C@@H]1[C@H]([C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1)O)C=1N=CNC=1)C(=O)C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C OYVAGSVQBOHSSS-UAPAGMARSA-O 0.000 claims description 4
- 150000002632 lipids Chemical class 0.000 claims description 4
- 239000003120 macrolide antibiotic agent Substances 0.000 claims description 4
- 229940041033 macrolides Drugs 0.000 claims description 4
- 229920001542 oligosaccharide Polymers 0.000 claims description 4
- 150000002482 oligosaccharides Chemical class 0.000 claims description 4
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 4
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 4
- 125000004309 pyranyl group Chemical group O1C(C=CC=C1)* 0.000 claims description 4
- 150000003431 steroids Chemical class 0.000 claims description 4
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 claims description 3
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 claims description 3
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims description 2
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 claims 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 claims 1
- 229930182817 methionine Natural products 0.000 claims 1
- 238000001727 in vivo Methods 0.000 abstract description 31
- 230000003197 catalytic effect Effects 0.000 abstract description 12
- 102000048120 Galactokinases Human genes 0.000 description 121
- 108700023157 Galactokinases Proteins 0.000 description 121
- 102000004190 Enzymes Human genes 0.000 description 39
- 108090000790 Enzymes Proteins 0.000 description 39
- 238000006243 chemical reaction Methods 0.000 description 24
- 235000021317 phosphate Nutrition 0.000 description 23
- 244000057717 Streptococcus lactis Species 0.000 description 20
- 238000003556 assay Methods 0.000 description 16
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 15
- 230000000694 effects Effects 0.000 description 13
- 229930014626 natural product Natural products 0.000 description 13
- LWFUFLREGJMOIZ-UHFFFAOYSA-N 3,5-dinitrosalicylic acid Chemical compound OC(=O)C1=CC([N+]([O-])=O)=CC([N+]([O-])=O)=C1O LWFUFLREGJMOIZ-UHFFFAOYSA-N 0.000 description 12
- 238000013459 approach Methods 0.000 description 10
- 239000000047 product Substances 0.000 description 10
- HEYJIJWKSGKYTQ-DPYQTVNSSA-N (2r,3s,4s,5r)-6-azido-2,3,4,5-tetrahydroxyhexanal Chemical compound [N-]=[N+]=NC[C@@H](O)[C@H](O)[C@H](O)[C@@H](O)C=O HEYJIJWKSGKYTQ-DPYQTVNSSA-N 0.000 description 9
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- 235000001014 amino acid Nutrition 0.000 description 8
- 150000002772 monosaccharides Chemical class 0.000 description 8
- 230000008569 process Effects 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 7
- 230000014509 gene expression Effects 0.000 description 7
- 239000013612 plasmid Substances 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- ULGZDMOVFRHVEP-RWJQBGPGSA-N Erythromycin Chemical compound O([C@@H]1[C@@H](C)C(=O)O[C@@H]([C@@]([C@H](O)[C@@H](C)C(=O)[C@H](C)C[C@@](C)(O)[C@H](O[C@H]2[C@@H]([C@H](C[C@@H](C)O2)N(C)C)O)[C@H]1C)(C)O)CC)[C@H]1C[C@@](C)(OC)[C@@H](O)[C@H](C)O1 ULGZDMOVFRHVEP-RWJQBGPGSA-N 0.000 description 6
- WQZGKKKJIJFFOK-VSOAQEOCSA-N L-altropyranose Chemical compound OC[C@@H]1OC(O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-VSOAQEOCSA-N 0.000 description 6
- 125000003275 alpha amino acid group Chemical group 0.000 description 6
- 238000012512 characterization method Methods 0.000 description 6
- 235000018102 proteins Nutrition 0.000 description 6
- 238000003786 synthesis reaction Methods 0.000 description 6
- HEYJIJWKSGKYTQ-JGWLITMVSA-N (2r,3s,4r,5r)-6-azido-2,3,4,5-tetrahydroxyhexanal Chemical compound [N-]=[N+]=NC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O HEYJIJWKSGKYTQ-JGWLITMVSA-N 0.000 description 5
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 5
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 5
- 125000000539 amino acid group Chemical group 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 5
- 230000008878 coupling Effects 0.000 description 5
- 238000010168 coupling process Methods 0.000 description 5
- 238000005859 coupling reaction Methods 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 108700014210 glycosyltransferase activity proteins Proteins 0.000 description 5
- 238000006366 phosphorylation reaction Methods 0.000 description 5
- 229930000044 secondary metabolite Natural products 0.000 description 5
- HDXVFGYNQCBFKD-DPYQTVNSSA-N (2r,3s,4s,5r)-6-amino-2,3,4,5-tetrahydroxyhexanal Chemical compound NC[C@@H](O)[C@H](O)[C@H](O)[C@@H](O)C=O HDXVFGYNQCBFKD-DPYQTVNSSA-N 0.000 description 4
- AOJJSUZBOXZQNB-TZSSRYMLSA-N Doxorubicin Chemical compound O([C@H]1C[C@@](O)(CC=2C(O)=C3C(=O)C=4C=CC=C(C=4C(=O)C3=C(O)C=21)OC)C(=O)CO)[C@H]1C[C@H](N)[C@H](O)[C@H](C)O1 AOJJSUZBOXZQNB-TZSSRYMLSA-N 0.000 description 4
- 238000006736 Huisgen cycloaddition reaction Methods 0.000 description 4
- WQZGKKKJIJFFOK-UHFFFAOYSA-N OCC1OC(O)C(O)C(O)C1O Chemical compound OCC1OC(O)C(O)C(O)C1O WQZGKKKJIJFFOK-UHFFFAOYSA-N 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 108010059993 Vancomycin Proteins 0.000 description 4
- 230000004075 alteration Effects 0.000 description 4
- 230000003321 amplification Effects 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 4
- 238000006911 enzymatic reaction Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000003199 nucleic acid amplification method Methods 0.000 description 4
- 230000037361 pathway Effects 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- DKVBOUDTNWVDEP-NJCHZNEYSA-N teicoplanin aglycone Chemical compound N([C@H](C(N[C@@H](C1=CC(O)=CC(O)=C1C=1C(O)=CC=C2C=1)C(O)=O)=O)[C@H](O)C1=CC=C(C(=C1)Cl)OC=1C=C3C=C(C=1O)OC1=CC=C(C=C1Cl)C[C@H](C(=O)N1)NC([C@H](N)C=4C=C(O5)C(O)=CC=4)=O)C(=O)[C@@H]2NC(=O)[C@@H]3NC(=O)[C@@H]1C1=CC5=CC(O)=C1 DKVBOUDTNWVDEP-NJCHZNEYSA-N 0.000 description 4
- MYPYJXKWCTUITO-LYRMYLQWSA-N vancomycin Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=C2C=C3C=C1OC1=CC=C(C=C1Cl)[C@@H](O)[C@H](C(N[C@@H](CC(N)=O)C(=O)N[C@H]3C(=O)N[C@H]1C(=O)N[C@H](C(N[C@@H](C3=CC(O)=CC(O)=C3C=3C(O)=CC=C1C=3)C(O)=O)=O)[C@H](O)C1=CC=C(C(=C1)Cl)O2)=O)NC(=O)[C@@H](CC(C)C)NC)[C@H]1C[C@](C)(N)[C@H](O)[C@H](C)O1 MYPYJXKWCTUITO-LYRMYLQWSA-N 0.000 description 4
- 229960003165 vancomycin Drugs 0.000 description 4
- MYPYJXKWCTUITO-UHFFFAOYSA-N vancomycin Natural products O1C(C(=C2)Cl)=CC=C2C(O)C(C(NC(C2=CC(O)=CC(O)=C2C=2C(O)=CC=C3C=2)C(O)=O)=O)NC(=O)C3NC(=O)C2NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(CC(C)C)NC)C(O)C(C=C3Cl)=CC=C3OC3=CC2=CC1=C3OC1OC(CO)C(O)C(O)C1OC1CC(C)(N)C(O)C(C)O1 MYPYJXKWCTUITO-UHFFFAOYSA-N 0.000 description 4
- FRJOXJBNQDZEBI-ZLUOBGJFSA-N (2r,3s,5s)-2,3,5,6-tetrahydroxyhexanal Chemical compound OC[C@@H](O)C[C@H](O)[C@@H](O)C=O FRJOXJBNQDZEBI-ZLUOBGJFSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 102000007390 Glycogen Phosphorylase Human genes 0.000 description 3
- 108010046163 Glycogen Phosphorylase Proteins 0.000 description 3
- 102100040648 L-fucose kinase Human genes 0.000 description 3
- 235000014897 Streptococcus lactis Nutrition 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 239000012043 crude product Substances 0.000 description 3
- 239000013078 crystal Substances 0.000 description 3
- 230000002255 enzymatic effect Effects 0.000 description 3
- 229960003276 erythromycin Drugs 0.000 description 3
- 239000013604 expression vector Substances 0.000 description 3
- 239000007850 fluorescent dye Substances 0.000 description 3
- 108010083136 fucokinase Proteins 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 230000013595 glycosylation Effects 0.000 description 3
- 238000006206 glycosylation reaction Methods 0.000 description 3
- 238000004128 high performance liquid chromatography Methods 0.000 description 3
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 3
- 230000001965 increasing effect Effects 0.000 description 3
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000002018 overexpression Effects 0.000 description 3
- 230000026731 phosphorylation Effects 0.000 description 3
- 239000012264 purified product Substances 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 238000002741 site-directed mutagenesis Methods 0.000 description 3
- PJTTXANTBQDXME-UGDNZRGBSA-N sucrose 6(F)-phosphate Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@]1(CO)[C@@H](O)[C@H](O)[C@@H](COP(O)(O)=O)O1 PJTTXANTBQDXME-UGDNZRGBSA-N 0.000 description 3
- 230000007704 transition Effects 0.000 description 3
- 230000007306 turnover Effects 0.000 description 3
- QJDMQMIMGQKBTN-DPYQTVNSSA-N (2R,3S,4S,5S)-4-azido-2,3,5,6-tetrahydroxyhexanal Chemical compound OC[C@@H](O)[C@H](N=[N+]=[N-])[C@H](O)[C@@H](O)C=O QJDMQMIMGQKBTN-DPYQTVNSSA-N 0.000 description 2
- 238000001644 13C nuclear magnetic resonance spectroscopy Methods 0.000 description 2
- 238000005160 1H NMR spectroscopy Methods 0.000 description 2
- 238000009010 Bradford assay Methods 0.000 description 2
- WQZGKKKJIJFFOK-WHZQZERISA-N D-aldose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-WHZQZERISA-N 0.000 description 2
- 238000001712 DNA sequencing Methods 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- WDJUZGPOPHTGOT-OAXVISGBSA-N Digitoxin Natural products O([C@H]1[C@@H](C)O[C@@H](O[C@@H]2C[C@@H]3[C@@](C)([C@@H]4[C@H]([C@]5(O)[C@@](C)([C@H](C6=CC(=O)OC6)CC5)CC4)CC3)CC2)C[C@H]1O)[C@H]1O[C@@H](C)[C@H](O[C@H]2O[C@@H](C)[C@@H](O)[C@@H](O)C2)[C@@H](O)C1 WDJUZGPOPHTGOT-OAXVISGBSA-N 0.000 description 2
- WQZGKKKJIJFFOK-ZNVMLXAYSA-N L-idopyranose Chemical compound OC[C@@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-ZNVMLXAYSA-N 0.000 description 2
- FXVPOMKTIZKCTJ-UHFFFAOYSA-N NCC1OC(O)C(O)C(O)C1O Chemical compound NCC1OC(O)C(O)C(O)C1O FXVPOMKTIZKCTJ-UHFFFAOYSA-N 0.000 description 2
- YJQPYGGHQPGBLI-UHFFFAOYSA-N Novobiocin Natural products O1C(C)(C)C(OC)C(OC(N)=O)C(O)C1OC1=CC=C(C(O)=C(NC(=O)C=2C=C(CC=C(C)C)C(O)=CC=2)C(=O)O2)C2=C1C YJQPYGGHQPGBLI-UHFFFAOYSA-N 0.000 description 2
- 208000022639 SchC6pf-Schulz-Passarge syndrome Diseases 0.000 description 2
- 208000001364 Schopf-Schulz-Passarge syndrome Diseases 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 239000000370 acceptor Substances 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- HXXFSFRBOHSIMQ-VFUOTHLCSA-N alpha-D-glucose 1-phosphate Chemical compound OC[C@H]1O[C@H](OP(O)(O)=O)[C@H](O)[C@@H](O)[C@@H]1O HXXFSFRBOHSIMQ-VFUOTHLCSA-N 0.000 description 2
- 230000003466 anti-cipated effect Effects 0.000 description 2
- 229930195731 calicheamicin Natural products 0.000 description 2
- HXCHCVDVKSCDHU-LULTVBGHSA-N calicheamicin Chemical compound C1[C@H](OC)[C@@H](NCC)CO[C@H]1O[C@H]1[C@H](O[C@@H]2C\3=C(NC(=O)OC)C(=O)C[C@](C/3=C/CSSSC)(O)C#C\C=C/C#C2)O[C@H](C)[C@@H](NO[C@@H]2O[C@H](C)[C@@H](SC(=O)C=3C(=C(OC)C(O[C@H]4[C@@H]([C@H](OC)[C@@H](O)[C@H](C)O4)O)=C(I)C=3C)OC)[C@@H](O)C2)[C@@H]1O HXCHCVDVKSCDHU-LULTVBGHSA-N 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 239000003054 catalyst Substances 0.000 description 2
- PBAYDYUZOSNJGU-UHFFFAOYSA-N chelidonic acid Natural products OC(=O)C1=CC(=O)C=C(C(O)=O)O1 PBAYDYUZOSNJGU-UHFFFAOYSA-N 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 229960000648 digitoxin Drugs 0.000 description 2
- YQICPRLGRXWDHI-XUDUSOBPSA-N digitoxin(1-) Chemical compound C[C@H]([C@H]([C@H](C1)O)O)O[C@H]1O[C@H]([C@@H](C)O[C@H](C1)O[C@H]([C@@H](C)O[C@H](C2)O[C@@H](CC3)C[C@@H](CC4)[C@@]3(C)[C@@H](CC[C@]3(C)[C@H](CC5)C([CH-]O6)=CC6=O)[C@@H]4[C@]35O)[C@H]2O)[C@H]1O YQICPRLGRXWDHI-XUDUSOBPSA-N 0.000 description 2
- 239000012154 double-distilled water Substances 0.000 description 2
- 229960004679 doxorubicin Drugs 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000007876 drug discovery Methods 0.000 description 2
- HKSZLNNOFSGOKW-UHFFFAOYSA-N ent-staurosporine Natural products C12=C3N4C5=CC=CC=C5C3=C3CNC(=O)C3=C2C2=CC=CC=C2N1C1CC(NC)C(OC)C4(C)O1 HKSZLNNOFSGOKW-UHFFFAOYSA-N 0.000 description 2
- 238000007824 enzymatic assay Methods 0.000 description 2
- 238000001215 fluorescent labelling Methods 0.000 description 2
- 239000010931 gold Substances 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- 238000011065 in-situ storage Methods 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 2
- 238000012933 kinetic analysis Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 description 2
- QCAWEPFNJXQPAN-UHFFFAOYSA-N methoxyfenozide Chemical compound COC1=CC=CC(C(=O)NN(C(=O)C=2C=C(C)C=C(C)C=2)C(C)(C)C)=C1C QCAWEPFNJXQPAN-UHFFFAOYSA-N 0.000 description 2
- 230000003278 mimic effect Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000002703 mutagenesis Methods 0.000 description 2
- 231100000350 mutagenesis Toxicity 0.000 description 2
- 231100000219 mutagenic Toxicity 0.000 description 2
- 230000003505 mutagenic effect Effects 0.000 description 2
- YJQPYGGHQPGBLI-KGSXXDOSSA-N novobiocin Chemical compound O1C(C)(C)[C@H](OC)[C@@H](OC(N)=O)[C@@H](O)[C@@H]1OC1=CC=C(C(O)=C(NC(=O)C=2C=C(CC=C(C)C)C(O)=CC=2)C(=O)O2)C2=C1C YJQPYGGHQPGBLI-KGSXXDOSSA-N 0.000 description 2
- 229960002950 novobiocin Drugs 0.000 description 2
- 229960000988 nystatin Drugs 0.000 description 2
- VQOXZBDYSJBXMA-NQTDYLQESA-N nystatin A1 Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/CC/C=C/C=C/[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 VQOXZBDYSJBXMA-NQTDYLQESA-N 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000002708 random mutagenesis Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000009738 saturating Methods 0.000 description 2
- 238000002864 sequence alignment Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000012064 sodium phosphate buffer Substances 0.000 description 2
- 239000007858 starting material Substances 0.000 description 2
- CGPUWJWCVCFERF-UHFFFAOYSA-N staurosporine Natural products C12=C3N4C5=CC=CC=C5C3=C3CNC(=O)C3=C2C2=CC=CC=C2N1C1CC(NC)C(OC)C4(OC)O1 CGPUWJWCVCFERF-UHFFFAOYSA-N 0.000 description 2
- HKSZLNNOFSGOKW-FYTWVXJKSA-N staurosporine Chemical compound C12=C3N4C5=CC=CC=C5C3=C3CNC(=O)C3=C2C2=CC=CC=C2N1[C@H]1C[C@@H](NC)[C@@H](OC)[C@]4(C)O1 HKSZLNNOFSGOKW-FYTWVXJKSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 125000001424 substituent group Chemical group 0.000 description 2
- 229910052717 sulfur Inorganic materials 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000004809 thin layer chromatography Methods 0.000 description 2
- JQOJJZURZJPKPF-UISOVIGQSA-N (2r,3s,4r,5s)-2,3,4,5,6,7-hexahydroxyhept-6-enal Chemical compound OC=C(O)[C@@H](O)[C@H](O)[C@H](O)[C@@H](O)C=O JQOJJZURZJPKPF-UISOVIGQSA-N 0.000 description 1
- SVSVZKHLDCZLNK-RSJOWCBRSA-N (2r,3s,4r,5s)-6,6-difluoro-2,3,4,5-tetrahydroxyhexanal Chemical compound O=C[C@H](O)[C@@H](O)[C@@H](O)[C@H](O)C(F)F SVSVZKHLDCZLNK-RSJOWCBRSA-N 0.000 description 1
- GGVVBDUYWFUSBV-DPYQTVNSSA-N (2r,3s,4r,5s)-6-bromo-2,3,4,5-tetrahydroxyhexanal Chemical compound BrC[C@@H](O)[C@H](O)[C@H](O)[C@@H](O)C=O GGVVBDUYWFUSBV-DPYQTVNSSA-N 0.000 description 1
- ZHVNVPHMOCWHHO-DPYQTVNSSA-N (2r,3s,4r,5s)-6-chloro-2,3,4,5-tetrahydroxyhexanal Chemical compound ClC[C@@H](O)[C@H](O)[C@H](O)[C@@H](O)C=O ZHVNVPHMOCWHHO-DPYQTVNSSA-N 0.000 description 1
- 238000004679 31P NMR spectroscopy Methods 0.000 description 1
- 101000910055 Arabidopsis thaliana Cycloartenol synthase Proteins 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 101000928995 Caenorhabditis elegans Putative deoxyribose-phosphate aldolase Proteins 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 102100037802 Deoxyribose-phosphate aldolase Human genes 0.000 description 1
- 241000588722 Escherichia Species 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 229920002527 Glycogen Polymers 0.000 description 1
- 102000005548 Hexokinase Human genes 0.000 description 1
- 108700040460 Hexokinases Proteins 0.000 description 1
- SHZGCJCMOBCMKK-PQMKYFCFSA-N L-Fucose Natural products C[C@H]1O[C@H](O)[C@@H](O)[C@@H](O)[C@@H]1O SHZGCJCMOBCMKK-PQMKYFCFSA-N 0.000 description 1
- SHZGCJCMOBCMKK-DHVFOXMCSA-N L-fucopyranose Chemical group C[C@@H]1OC(O)[C@@H](O)[C@H](O)[C@@H]1O SHZGCJCMOBCMKK-DHVFOXMCSA-N 0.000 description 1
- 241000194036 Lactococcus Species 0.000 description 1
- 108010059597 Lanosterol synthase Proteins 0.000 description 1
- 102100032011 Lanosterol synthase Human genes 0.000 description 1
- 229910021380 Manganese Chloride Inorganic materials 0.000 description 1
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 description 1
- 102000048245 N-acetylneuraminate lyases Human genes 0.000 description 1
- 108700023220 N-acetylneuraminate lyases Proteins 0.000 description 1
- MSWZFWKMSRAUBD-UHFFFAOYSA-N NC1C(O)OC(CO)C(O)C1O Chemical compound NC1C(O)OC(CO)C(O)C1O MSWZFWKMSRAUBD-UHFFFAOYSA-N 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 239000012564 Q sepharose fast flow resin Substances 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000187560 Saccharopolyspora Species 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- RJDIFQMDPPUATQ-UHFFFAOYSA-N [H]C1C(O)C(O)OC(CO)C1O Chemical compound [H]C1C(O)C(O)OC(CO)C1O RJDIFQMDPPUATQ-UHFFFAOYSA-N 0.000 description 1
- PMMURAAUARKVCB-UHFFFAOYSA-N [H]C1C(O)OC(CO)C(O)C1O Chemical compound [H]C1C(O)OC(CO)C(O)C1O PMMURAAUARKVCB-UHFFFAOYSA-N 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- GZCGUPFRVQAUEE-ZXXMMSQZSA-N aldehydo-D-idose Chemical compound OC[C@@H](O)[C@H](O)[C@@H](O)[C@H](O)C=O GZCGUPFRVQAUEE-ZXXMMSQZSA-N 0.000 description 1
- HXXFSFRBOHSIMQ-FPRJBGLDSA-N alpha-D-galactose 1-phosphate Chemical compound OC[C@H]1O[C@H](OP(O)(O)=O)[C@H](O)[C@@H](O)[C@H]1O HXXFSFRBOHSIMQ-FPRJBGLDSA-N 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 238000005349 anion exchange Methods 0.000 description 1
- 238000005571 anion exchange chromatography Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000002924 anti-infective effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000010462 azide-alkyne Huisgen cycloaddition reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- PTVXQARCLQPGIR-SXUWKVJYSA-N beta-L-fucose 1-phosphate Chemical compound C[C@@H]1O[C@H](OP(O)(O)=O)[C@@H](O)[C@H](O)[C@@H]1O PTVXQARCLQPGIR-SXUWKVJYSA-N 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000006696 biosynthetic metabolic pathway Effects 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- 230000023852 carbohydrate metabolic process Effects 0.000 description 1
- 235000021256 carbohydrate metabolism Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 229940097217 cardiac glycoside Drugs 0.000 description 1
- 239000002368 cardiac glycoside Substances 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 108091036078 conserved sequence Proteins 0.000 description 1
- 150000004775 coumarins Chemical class 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 229940075258 d-tal Drugs 0.000 description 1
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 1
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 101150045500 galK gene Proteins 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 229950010772 glucose-1-phosphate Drugs 0.000 description 1
- 108010034416 glutarylamidocephalosporanic acid acylase Proteins 0.000 description 1
- 229940096919 glycogen Drugs 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000000021 kinase assay Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- DHRRIBDTHFBPNG-UHFFFAOYSA-L magnesium dichloride hexahydrate Chemical compound O.O.O.O.O.O.[Mg+2].[Cl-].[Cl-] DHRRIBDTHFBPNG-UHFFFAOYSA-L 0.000 description 1
- 239000011565 manganese chloride Substances 0.000 description 1
- 235000002867 manganese chloride Nutrition 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 235000019799 monosodium phosphate Nutrition 0.000 description 1
- 230000000869 mutational effect Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 239000011535 reaction buffer Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 229930002534 steroid glycoside Natural products 0.000 description 1
- 150000008143 steroidal glycosides Chemical class 0.000 description 1
- 229940124530 sulfonamide Drugs 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- 108010058071 tagatose 1,6-diphosphate aldolase Proteins 0.000 description 1
- 238000004885 tandem mass spectrometry Methods 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 238000006257 total synthesis reaction Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
Definitions
- This invention generally relates to sugar kinases and specifically to novel anomeric D/L sugar kinases with expanded substrate specificity and methods of use.
- in vitro glycorandomization makes use of the inherent or engineered substrate promiscuity of nucleotidylyltransferases and glycosyltransferases to activate and attach chemically synthesized sugar precursors to various natural product scaffolds. This efficiently incorporates highly functionalized “unnatural” sugar substitutions into the corresponding natural product scaffold ( FIG. 1 b ).
- the present invention provides sugar kinases with expanded substrate specificity and methods of use.
- One embodiment of the present invention provides a GalK variant for in vivo glycorandomization selected from the group consisting of a Y371H, M173L and Y371H-M173L mutations.
- the GalK variant displays substrate specificity toward a D or L sugar.
- the D or L sugar may be selected from the group consisting of D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose.
- Another embodiment of the present invention provides a method of providing a sugar phosphate.
- the method comprises the step of incubating a nucleotide triphosphate (NTP) and a D or L sugar in the presence of a GalK variant as discussed in the above paragraph, such that a sugar phosphate is produced.
- NTP nucleotide triphosphate
- the D or L sugar includes galactose or glucose-configured sugars having substitutions at C-2, C-3, C-4, C-6 positions.
- the D or L sugar include D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose.
- Yet another embodiment of the present invention provides an E. coli GalK variant of the wild type amino acid sequence of SEQ ID NO: 1 wherein the wild type amino acid sequence is mutated at one or more amino acid residues.
- the mutations are selected from the group consisting of R28, E34, D37, D174, Y223, C339, Y371, Y371H, M173, M173L and C353.
- This variant is capable of displaying catalytic activity toward a D or L sugar selected from the group consisting of D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose.
- the GalK variant is Y371H-M173L.
- Another embodiment of the present invention provides a method of phosphorylating sugars.
- This method comprises the step of incubating a nucleotide triphosphate (NTP) and a D or L sugar in the presence of a GalK variant according as discussed above, such that a sugar phosphate is produced.
- NTP nucleotide triphosphate
- the NTP is ATP.
- the method is carried out in a host cell.
- D or L sugar in this method is selected from the group consisting of D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose.
- Yet another aspect of the present invention provides a method of synthesizing an NDP-sugar.
- This method comprises the steps of: (a) incubating a nucleotide triphosphate (NTP) and a D or L sugar in the presence of a GalK variant as discussed, whereby a sugar phosphate is produced; and (b) incubating the sugar phosphate with a nucleotidylyltransferase, such that a NDP-sugar is produced.
- NDP nucleotide triphosphate
- the D or L sugar is selected from the group consisting of D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose.
- the nucleotidylyltransferase is Ep or a mutated variant thereof.
- the mutated Ep variant includes an Ep mutated at one or more amino acids selected from the group consisting of V173, G147, W224, N112, G175, D111, E162, T201, I200, E199, R195, L89, L89T, L109, Y146 and Y177.
- the NTP is ATP.
- the GalK variant is Y371H, M173L or Y371H-M173L.
- This method may be carried out in vitro or in a host cell.
- the host cell is preferably a bacterium. More preferably, the host cell is selected from the group consisting of E. coli and S. lividans.
- Another aspect of the invention provides a method of producing a glycosylated biomolecule containing at least one sugar moiety.
- the method comprises the steps of: (a) incubating a nucleotide triphosphate (NTP) and a D or L sugar in the presence of a GalK variant such that a sugar phosphate is produced; (b) incubating the sugar phosphate with a nucleotidylyltransferase, such that a NDP-sugar is produced; and (c) incubating the NDP-sugar with a biomolecule capable of being glycosylated in the presence of a glycosyltransferase, whereby a glycosylated biomolecule is produced.
- NDP nucleotide triphosphate
- the D or L sugar is selected from the group consisting of D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose.
- the nucleotidylyltransferase is Ep or a mutated variant thereof.
- Mutated Ep variant includes Ep that is mutated at one or more amino acids selected from the group consisting of V173, G147, W224, N112, G175, D111, E162, T201, I200, E199, R195, L89, L89T, L109, Y146 and Y177.
- glycosyltransferase is selected from the group consisting of CalB, CalE, CalN, CalU, Gra orf14, Gra orf5, LanGT1, LanGT2, LanGT3, LanGT4, MtmGI, MtmGII, MtmGTIII, MtmGTIV, NovM, RhlB, Rif orf 7, SnogD, SnogE, SnogZ, UrdGT1a, UrdGT1b, UrdGT1c, UrdGT2, AknK, AknS, DesVII, DnrS, OleG1, OleG2, TylCV, TylMII, TylN, DauH, DnrH, EryBV, EryCIII, Ngt, BgtA, BgtB, BgtC, GftA, GftB, GftC, GftD, GftE, Gp1-1, Gp1-2, RtfA, AveBI, BlmE, BlmF, MgtA,
- the NTP is ATP.
- the GalK variant is Y371H, M173L or Y371H-M173L.
- This method may be carried out in vitro or in a host cell.
- the host cell is a bacterium. More preferably, the host cell is selected from the group consisting of E. coli and S. lividans .
- the biomolecule capable of being glycosylated is selected from the group consisting of natural and synthetic metabolites, pyran rings, furan rings, enediynes, anthracyclines, angucyclines, aureolic acids, orthosomycins, macrolides, aminoglycosides, non-ribosomal peptides, polyenes, steroids, lipids, indolocarbazoles, bleomycins, amicetins, benzoisochromanequinones coumarins, polyketides, pluramycins, aminoglycosides, oligosaccharides, peptides, proteins, hybrids consisting of one or more these components, analogs and bioactive aglycons thereof.
- the glycosylated biomolecule is further incubated with at least one chemoselectively ligatable moiety, such that at least one chemoselectively ligated compound is produced.
- FIG. 1 a) Provides representative examples for natural product glycosides used as therapeutics: calicheamicin (1), doxorubicin (2), erythromycin (3), staurosporine (4), vancomycin (5), nystatin (6), novobiocin (7), and digitoxin (8).
- the attached sugars are highlighted in color with red indicating L-configured sugars, and blue representing D-sugars.
- E p denotes ⁇ -D-glucopyranosyl phosphate thymidylyltransferase, GlyT n different glycosyltransferases.
- FIG. 2 Provides reactions catalyzed by anomeric kinases. a) Glycogen phosphorylase. b) Fucokinase. c) Galactokinase. d) Proposed phosphorylation of L-altrose accomplished by the evolved GalK mutant Y371H.
- FIG. 3 Provides representative quantitative data for a set of GalK variants, illustrating screen for D-galacturonic acid (X-axis) and L-altrose (Y-axis). The higher the loss in absorption (shown in absorption units (AU), the more active the enzyme variant.
- FIG. 4 Provides 3 J H-H coupling patterns and NOESY correlations for the GalK Y371H product ⁇ -L-altrose-1-phosphate.
- FIG. 5 Glycorandomization overview and two potential scenarios for an in vivo approach.
- In vitro glycorandomization utilizes two enhanced enzymes—E 1 (a general kinase) and E 2 (a general nucleotidylyltransferase)—to generate NDP-sugar substrate libraries to be utilized by a flexible natural product-associated glycosyltransferase (GlyT).
- E 1 a general kinase
- E 2 a general nucleotidylyltransferase
- GlyT flexible natural product-associated glycosyltransferase
- In vivo glycorandomization scenario I feeding monosaccharides to a natural product-producing host engineered to express the ‘NDP-sugar factory’. In this scenario, both the aglycon and glycosyltransferase are provided by the bacterial host.
- FIG. 6 Comparison of D-glucose docked within the active site of L. lactis GalK and homology-model of E. coli GalK.
- FIG. 7 ‘Natural’ and ‘unnatural’ substrates of wild-type GalK and GalK mutants (M173L, Y371H and M173L-Y371H).
- FIG. 9 In vivo GalK-catalyzed sugar-1-phosphate production.
- glycogen phosphorylases which convert glycogen (9) into D-glucose-1-phosphate (10)
- fucokinases which transfer a phosphate from ATP to the anomeric position of L-fucose (12) to provide ⁇ -L-fucose-1-phosphate (13)
- galactokinases GalK
- Structure-based engineering relies upon knowledge of an enzyme's three dimensional structure and an explicit molecular-level understanding of substrate recognition.
- An example of structure-based engineering as applied to IVG includes increasing the substrate scope of nucleotidylyltransferases employed ( FIG. 1 b , “Ep”).
- Ep rational engineering led to active site mutants capable of accepting a variety of substrates not utilized by the wild-type enzyme.
- Such techniques are also described in the U.S. Patent Publications 2003/0055235A1 and 2003/0068669A1, and International Publications WO02079150 and WO0248331, which are incorporated herein by reference in their entirety for all purposes.
- in vitro evolution strategies include error-prone PCR for gene diversification, and/or locating critical amino acid residues for saturation mutagenesis or, more prominently, shuffling of fragmented diversified genes or gene families according to a number of different protocols. Subsequently, the diversified proteins are subjected to a screen. In a recent demonstration of IVG, >50 analogs of 5 (vancomycin) were generated, some of which displayed enhanced and distinct antibacterial profiles from the parent natural product.
- the present invention provides sugar kinases with expanded substrate specificity and methods of use.
- One embodiment of the present invention provides a GalK variant for in vivo glycorandomization selected from the group consisting of a Y371H, M173L and Y371H-M173L mutation.
- the GalK variant displays substrate specificity toward a D or L sugar.
- the D or L sugar may be selected from the group consisting of D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose.
- Another embodiment of the present invention provides a method of providing a sugar phosphate.
- the method comprises the step of incubating a nucleotide triphosphate (NTP) and a D or L sugar in the presence of a GalK variant according to claim 1 , such that a sugar phosphate is produced.
- NTP nucleotide triphosphate
- the D or L sugar includes galactose or glucose-configured sugars having substitutions at C-2, C-3, C-4, C-6 positions.
- the D or L sugar include D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose.
- Yet another embodiment of the present invention provides an E. coli GalK variant of the wild type amino acid sequence of SEQ ID NO: 1 wherein the wild type amino acid sequence is mutated at one or more amino acid residues.
- the mutations are selected from the group consisting of R28, E34, D37, D174, Y223, C339, Y371, Y371H, M173, M173L and C353.
- This variant is capable of displaying catalytic activity toward a D or L sugar selected from the group consisting of D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose.
- the GalK variant is Y371H-M173L.
- Another embodiment of the present invention provides a method of phosphorylating sugars.
- This method comprises the step of incubating a nucleotide triphosphate (NTP) and a D or L sugar in the presence of a GalK variant according as discussed above, such that a sugar phosphate is produced.
- NTP nucleotide triphosphate
- the NTP is ATP.
- the method is carried out in a host cell.
- D or L sugar in this method is selected from the group consisting of D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose.
- Yet another aspect of the present invention provides a method of synthesizing an NDP-sugar.
- This method comprises the steps of: (a) incubating a nucleotide triphosphate (NTP) and a D or L sugar in the presence of a GalK variant as discussed, whereby a sugar phosphate is produced; and (b) incubating the sugar phosphate with a nucleotidylyltransferase, such that a NDP-sugar is produced.
- NDP nucleotide triphosphate
- the D or L sugar is selected from the group consisting of D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose.
- the nucleotidylyltransferase is Ep or a mutated variant thereof.
- the mutated Ep variant includes an Ep mutated at one or more amino acids selected from the group consisting of V173, G147, W224, N112, G175, D111, E162, T201, I200, E199, R195, L89, L89T, L109, Y146 and Y177.
- the NTP is ATP.
- the GalK variant is Y371H, M173L or Y371H-M173L.
- This method may be carried out in vitro or in a host cell.
- the host cell is preferably a bacterium. More preferably, the host cell is selected from the group consisting of E. coli and S. lividans.
- Another aspect of the invention provides a method of producing a glycosylated biomolecule containing at least one sugar moiety.
- the method comprises the steps of: (a) incubating a nucleotide triphosphate (NTP) and a D or L sugar in the presence of a GalK variant such that a sugar phosphate is produced; (b) incubating the sugar phosphate with a nucleotidylyltransferase, such that a NDP-sugar is produced; and (c) incubating the NDP-sugar with a biomolecule capable of being glycosylated in the presence of a glycosyltransferase, whereby a glycosylated biomolecule is produced.
- NDP nucleotide triphosphate
- the D or L sugar is selected from the group consisting of D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose.
- the nucleotidylyltransferase is Ep or a mutated variant thereof.
- Mutated Ep variant includes Ep that is mutated at one or more amino acids selected from the group consisting of V173, G147, W224, N112, G175, D111, E162, T201, I200, E199, R195, L89, L89T, L109, Y146 and Y177.
- glycosyltransferase is selected from the group consisting of CalB, CalE, CalN, CalU, Gra orf14, Gra orf5, LanGT1, LanGT2, LanGT3, LanGT4, MtmGI, MtmGII, MtmGTIII, MtmGTIV, NovM, RhlB, R1f orf 7, SnogD, SnogE, SnogZ, UrdGT1a, UrdGT1b, UrdGT1c, UrdGT2, AknK, AknS, DesVII, DnrS, OleG1, OleG2, TylCV, TylMII, TylN, DauH, DnrH, EryBV, EryCIII, Ngt, BgtA, BgtB, BgtC, GftA, GftB, GftC, GftD, GftE, Gp1-1, Gp1-2, RtfA, AveBI, BlmE, BlmF, MgtA,
- the NTP is ATP.
- the GalK variant is Y371H, M173L or Y371H-M173L.
- This method may be carried out in vitro or in a host cell.
- the host cell is a bacterium. More preferably, the host cell is selected from the group consisting of E. coli and S. lividans .
- the biomolecule capable of being glycosylated is selected from the group consisting of natural and synthetic metabolites, pyran rings, furan rings, enediynes, anthracyclines, angucyclines, aureolic acids, orthosomycins, macrolides, aminoglycosides, non-ribosomal peptides, polyenes, steroids, lipids, indolocarbazoles, bleomycins, amicetins, benzoisochromanequinones coumarins, polyketides, pluramycins, aminoglycosides, oligosaccharides, peptides, proteins, hybrids consisting of one or more these components, analogs and bioactive aglycons thereof.
- the glycosylated biomolecule is further incubated with at least one chemoselectively ligatable moiety, such that at least one chemoselectively ligated compound is produced.
- E. coli strains XL1-blue and BL21-Gold were purchased from Stratagene (La Jolla, Calif.). The template plasmid pGalK has been previously described.
- Expression vector pET15b was from Novagen (Madison, Wis.). All reagent grade chemicals and enzymes were purchased from Promega (Madison, Wis.), Sigma (St. Louis, Mo.), Fisher/Acros Organics (Hanover Park, Ill.), or Fluka (Milwaukee, Wis.).
- the amplification products were digested with BamHI/XbaI, purified on an agarose gel (0.8% w/vol), eluted using the QIAquick extraction kit (QIAGEN, Valencia, Calif.), ligated into appropriately digested pET15b, to directly transform E. coli XL1 blue. Plasmids were isolated from randomly picked colonies ( ⁇ 20 representatives for each library generated) and sequenced on an ABI 310 automatic DNA sequencer (PerkinElmer, Foster City, Calif.). Upon verifying the desired mutation rate, all transformants were pooled, cultured overnight, the collectively recovered plasmids used to transform E. coli BL21-Gold (DE3) and the library processed as described below.
- E. coli Bacterial fermentation. E. coli was grown in LB medium, supplemented with ampicillin (100 ⁇ L mL-1 final) under standard conditions.
- individual transformants were grown as 1 mL miniature cultures in 96 deep well blocks overnight as seed cultures, then replicated in fresh medium (2%, vol/vol), afterwards added 15% (vol/vol) glycerol, mixed, and stored at 80° C.
- the replicated cultures were grown to an OD600 ⁇ 0.7, protein expression was then induced by adding 1 mM IPTG (final), for 2 hours, then harvested by centrifugation (10 min, 3000 ⁇ g). The cell paste was frozen at 20° C.
- the biomass of each well was resuspended in 50 ⁇ L NPI-buffer (50 mM NaH2PO4, 300 mM NaCl, pH 8.0) to which was then added 70 ⁇ L NPI-buffer supplemented with 1 ⁇ L Lysonase (Novagen, Madison, Wis.) for lysis.
- Cell debris was collected by centrifugation (10 min, 3000 ⁇ g), and 20 ⁇ L of the clear supernatant, containing ⁇ 0.5 ⁇ g of the expressed GalK variant on average, was used for each kinase assay.
- Enzymatic reactions and assays were set up and read in 96 well format on a Biomek FX automated liquid handling workstation (Beckman Coulter, Fullerton, Calif.) fitted to a Fluostar Optima plate reader (BMG, Durham, N.C.).
- the in vitro enzymatic reactions and assays followed the protocol published previously, slightly modified for automated liquid handling: 150 ⁇ L sugar solution (8 mM final) and 12 ⁇ L ATP/Mg2+ solution (20 mM/5 mM final) were mixed, preincubated at 37° C. for 5 min, then 20 ⁇ L of the cleared supernatant was added, and the reaction incubated at 37° C. for an additional 2 h.
- GalK mutant Y371H was overexpressed and purified following the procedure previously described for wild-type GalK.
- the fractions containing homogenous Y371H GalK were collected, concentrated and quantified using the Bradford protein assay.
- the DNS assay was used to assess the substrate specificity of the purified GalK mutant (Y371H) as previously described. Standard curves for each sugar were prepared as described. In order to determine the kinetic data for each active monosaccharide substrate, the sugar concentration was varied over a range of 1-16 mM, under saturating ATP (15 mM).
- L-altrose (21.6 mg, 0.12 mmol) was dissolved in 15 mL 50 mM sodium phosphate buffer (pH 7.5). To this solution, ATP (125 mg, 0.23 mmol), MgCl2.6H2O (15.3 mg, 0.07 mmol) were added, the mixture incubated at 37° C. for 5 min, the reaction initiated via the addition of enzyme (Y371H) to a final concentration of 150 ⁇ g mL-1 and reaction progress monitored by TLC.
- ATP 125 mg, 0.23 mmol
- MgCl2.6H2O (15.3 mg, 0.07 mmol
- GalK Directed evolution of GalK with expanded specificity.
- the cloned wild-type galK gene from E. coli was subjected to random mutagenesis by epPCR performed over the entire gene.
- the level of sequence alteration was adjusted to an average of 1.5 amino acid substitutions per enzyme molecule, and verified by DNA-sequencing of corresponding genes.
- epPCR usually results in a more or less strong mutational bias (29, 30, 44) and is therefore not a truly random process.
- the library contained a transition/transversion ratio of ⁇ 3.0, the transitions outnumbering the transversions, and the AT ⁇ GC/GC ⁇ AT ratio was found to be 2.4.
- GalK variants from this library were evaluated for their ability to accommodate an expanded spectrum of sugar substrates.
- the inventors' goals were kinase activity toward L-configured sugars (C-5 alteration) or to those with altered substituents at C-6.
- the inventors' recently developed DNS assay is universally applicable to all reducing sugars. Consequently, simultaneous screens for a relaxed enzyme specificity were carried out with a set of appropriately selected sugar substrates rather than a one-dimensional single substrate screen of each library member.
- FIG. 3 The graphical display of a typical screening result is given in FIG. 3 .
- two GalK variants appeared independently which phosphorylated the C-5/C-6 set of sugars with roughly similar efficiency.
- Subsequent DNA-sequencing of both GalK variant genes revealed that they were identical in their sequence and carried a single forward mutation, a C ⁇ T transition at position 1111 of the wild-type reading frame, which translates into a tyrosine 371 to histidine replacement.
- Lactococcus ( L .) lactis galactokinase was published. Despite a rather low sequence homology to the E. coli GalK (36% identity, 53% similarity) these two kinases clearly share three characteristic footprint motifs, and all amino acid residues found within the catalytic center of the L. lactis galactokinase (R36, E42, D45, D183, and Y233) are invariably present in its E. coli homolog (R28, E34, D37, D1174, Y223, respectively), embedded in highly conserved sequence environments. For this reason, the inventors speculate that the equivalent residues also form the catalytic apparatus in the E. coli enzyme.
- residue 371 in the E. coli wild-type enzyme found to be essential for the widened substrate specificity and the activity toward L-configured sugars, does not appear as part of its deduced active site.
- the C ⁇ of the equivalent amino acid Y385 located within the C-terminal domain ⁇ -strand K is ⁇ 20 ⁇ from the anomeric carbon of the substrate when bound in the active site.
- Y385 is located in close proximity to C353 (C339 in E. coli GalK).
- the tyrosine phenolic oxygen is located ⁇ 5.5 ⁇ from the cysteine sulfur atom.
- GalK variant Characterization of the GalK variant (Y371H).
- Y371H a sugar library of twenty putative substrates was tested with the purified enzyme.
- both the DNS assay and thin layer chromatography were used to monitor the reaction progress and control assays in the absence of enzyme or sugar were performed in parallel.
- the mutant GalK demonstrated the ability to turn over compounds 14, 16, 18-25 (Table 1), strikingly expanding the overall substrate scope compared with wild-type E. coli GalK.
- the kinetic parameters of the mutant enzyme with all active substrates were determined using the DNS assay and compared with wild-type GalK activity.
- GalK catalytic activity and substrate selectivity was accomplished in the initial round of random mutagenesis.
- Other recent similar examples of single forward mutations leading to a catalytic shift include, for example, the Arabidopsis thaliana cycloartol synthase or yeast lanosterol synthase, or the adipyl acylase evolved from a Pseudomonas glutaryl acylase. From an analysis of the GalK substrate specificity profiles, one can begin to construct a loose structure-activity requirement for both wild-type enzyme and the corresponding Y371H mutant.
- wild-type GalK displays a stringent requirement for the substrate galactose architecture from C-3 through C-6 and is capable of limited flexibility toward substitution at C-2. Yet, it is interesting to note these stringent requirements, with the exception of the extensive contacts at C-4, are not readily apparent in the L. lactis GalK active site structure.
- the Y371H mutation retains primarily only the stringent requirement for the C-4 galactose architecture with an enhanced substrate specificity flexibility at all other positions of the sugar.
- an enhancement of catalytic efficiency was observed in the Y371H mutant, the enhancement ranging from 5- to 22-fold. The only exception was the wild-type substrate galactose for which the catalytic efficiency was decreased slightly in comparison to wild-type GalK, albeit kcat in this case was also increased 2-fold.
- L-sugar conversion Confirmation of L-sugar conversion.
- the substrate specificity studies have demonstrated GalK variant Y371H to be a D/L-unspecific sugar kinase.
- a representative L-sugar reaction product was further characterized. Specifically, a small-scale preparative phosphorylation reaction was performed with L-altrose (21.6 mg, 0.12 mmol). The DNS assay indicated 91% of L-altrose conversion within four hours. Product isolation was readily achieved by anion exchange chromatography, and the yield of purified product was ⁇ 52%.
- the purified product was characterized by 1H and 13C NMR from which H—H coupling and NOESY data confirmed the product to be ⁇ -L-altrose-1-phosphatein a 1C4 conformation ( FIG. 4 ).
- 3JH-H coupling data showed two typical axial-equatorial couplings (H1-H2, H3-H4) and one equatorial-equatorial coupling (H2-H3).
- NOESY data also revealed the anticipated correlations consistent with this structure (H1-H2, H1-H5, H3-H4, H4-H6 and H5-H6).
- the inventors propose the Y371H mutant must bind and phosphorylate L-altrose in the same 4C1 conformation as D-galactose ( FIG. 2 c and 2 d ) (38) the product of which subsequently rapidly equilibrates to the more stable 1C4 conformation upon release from the enzyme.
- kinase-enhanced IVG not only simplifies the upstream availability of sugar-1-phosphates for IVG but also potentially opens the door to in vivo applications of glycorandomization.
- the expression of a tandem promiscuous sugar-1-kinase (GalK) and nucleotidylyltransferase (Ep) in a given organism presents the prospect of generating a library of NDP-sugars in situ.
- the present invention provides the foundation for eventually glycorandomizing a variety of clinically important secondary metabolites in vivo to rapidly enhance drug discovery efforts.
- Such techniques are also described in the U.S. Patent Publications 2003/0055235A1 and 2003/0068669A1, and International Publications WO02079150 and WO0248331, which are incorporated herein by reference in their entirety for all purposes.
- promiscuous sugar-1-kinase may be used for synthesizing NDP-sugars.
- the method of synthesizing comprises the steps of incubating a nucleotide triphosphate (NTP) and a D or L sugar in the presence of a GalK variant such that a sugar phosphate is produced.
- NTP nucleotide triphosphate
- the sugar phosphate is further incubated with a nucleotidylyltransferase, such that a NDP-sugar is produced.
- Various anomeric sugars may be used to form the sugar phosphate, including the D or L sugars such as D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose.
- D or L sugars such as D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose.
- the nucleotidylyltransferase is Ep or a mutated variant thereof.
- the mutated Ep variant includes Ep that is mutated at one or more amino acids V173, G147, W224, N112, G175, D111, E162, T201, I200, E199, R195, L89, L89T, L109, Y146 and Y177.
- the method may be carried out in vitro.
- the method is carried out in a host cell.
- the host cell may be a bacterium. Further, the host cell may be selected from the group consisting of E. coli and S. lividans.
- Another preferred embodiment of the present invention provides a method of producing a glycosylated biomolecule containing at least one sugar moiety.
- the method comprises the steps of incubating a nucleotide triphosphate (NTP) and a D or L sugar in the presence of a GalK variant, whereby a sugar phosphate is produced; incubating the sugar phosphate with a nucleotidylyltransferase, whereby a NDP-sugar is produced; and incubating the NDP-sugar with a biomolecule capable of being glycosylated in the presence of a glycosyltransferase, such that a glycosylated biomolecule is produced.
- NDP nucleotide triphosphate
- the glycosyltransferase is selected from the group consisting of CalB, CalE, CalN, CalU, Gra orf14, Gra orf5, LanGT1, LanGT2, LanGT3, LanGT4, MtmGI, MtmGII, MtmGTIII, MtmGTIV, NovM, RhlB, Rif orf 7, SnogD, SnogE, SnogZ, UrdGT1a, UrdGT1b, UrdGT1c, UrdGT2, AknK, AknS, DesVII, DnrS, OleG1, OleG2, TylCV, TylMII, TylN, DauH, DnrH, EryBV, EryCIII, Ngt, BgtA, BgtB, BgtC, GftA, GftB, GftC, GftD, GftE, Gp1-1, Gp1-2, RtfA, AveBI, BlmE, BlmF,
- the biomolecule capable of being glycosylated is selected from the group consisting of natural and synthetic metabolites, pyran rings, furan rings, enediynes, anthracyclines, angucyclines, aureolic acids, orthosomycins, macrolides, aminoglycosides, non-ribosomal peptides, polyenes, steroids, lipids, indolocarbazoles, bleomycins, amicetins, benzoisochromanequinones coumarins, polyketides, pluramycins, aminoglycosides, oligosaccharides, peptides, proteins, hybrids consisting of one or more these components, analogs and bioactive aglycons thereof.
- the glycosylated biomolecule is further incubated with at least one chemoselectively ligatable moiety, such that at least one chemoselectively ligated compound is produced.
- at least one chemoselectively ligatable moiety such that at least one chemoselectively ligated compound is produced.
- Glycorandomization ( FIG. 5 a ), a process centered upon the inherent promiscuity of secondary metabolite-associated glycosyltransferases, is one of the latest promising developments toward this important goal.
- Critical to the success of glycorandomization has been the ability to engineer and/or evolve two additional promiscuous enzymes—anomeric kinases and nucleotidylyltransferases.
- this two-enzyme short activation pathway allows one to rapidly diversify the sugars attached to complex natural products.
- the glycorandomization process is further enhanced via a final diversification step which relies upon the use of downstream chemoselective ligation.
- the erythromycin-producing Saccharopolyspora should present the prospect of generating a glycorandomized library in situ, the glycorandomized metabolite output of which is controlled by monosaccharides being fed to the strain ( FIG. 5 b ).
- expression of the tandem two gene ‘NDP-sugar factory’ genes in a non-producing host e.g. S. lividans or E. coli ) which expresses a given glycosyltransferase (or glycosyltransferase library)
- a non-producing host e.g. S. lividans or E. coli
- glycosyltransferase library should also provide a vehicle to accomplish glycorandomization via feeding the host with appropriate aglycon acceptors and unnatural sugar donors ( FIG. 5 c ).
- the inventor has applied directed enzyme evolution and relied upon a high throughput galactokinase (GalK) assay (DNS assay) for the screening of diverse E. coli GalK variant libraries generated via error-prone PCR.
- GalK galactokinase
- DTS assay high throughput galactokinase assay
- Y371H demonstrated remarkably widened substrate flexibility toward C-2, C-3 and C-5 substitutions of D-galactose.
- the mutant retained a stringent requirement for the axial C-4 galactose architecture.
- L. lactis GalK crystal structure suggested two highly conserved active-site residues (Asp-37 and Tyr-223 in E. coli GalK) are responsible for hydrogen-bonding with this C-4 axial hydroxyl group of the substrate.
- the inventor reports a structure-activity model, based upon the L. lactis active site and its ability to weakly utilize glucose, led to a specific engineered E. coli M 173L mutant GalK with enhanced C-4 and C-6 promiscuity. Moreover, a combination of the favorable structure-based (M173L) mutation with the beneficial mutation previously discovered via directed evolution (Y371H) drastically exceeds an additive enhancement for both C-4 and C-6 substitutions. Most importantly, the additional unnatural sugar substrates accessed by this unique double mutant allowed the unique opportunity to assess whether unnatural sugars can enter a bacterial host and serve as efficient substrates of the first enzyme of the glycorandomization pathway (the flexible anomeric kinase).
- E. coli strains XL1-blue and BL21 (DE3) were purchased from Stratagene (La Jolla, Calif.).
- Expression vector pET15b was purchased from Novagen (Madison, Wis.). Enzymes were purchased from Promega (Madison, Wis.).
- 6-thio-6-deoxy-D-galactose 43 6-thio-6-deoxy-D-glucose 44, 6-azido-6-deoxy-D-glucose 46, and 5-dimethylaminonaphthalene-1-(N-(5-propargylamidepentyl))-sulfonamide 50, see supporting methods, which is published as supporting information on the PNAS web site.
- the PDB file for the crystal structure of L. lactis wild type GalK was obtained from the Protein Data Bank, Research Collaboratory for Structural Bioinformatics, Rutgers University, New Brunswick, N.J. (available on the Rutgers University Website).
- the structure modeling was accomplished using Swiss-PdbViewer software (Version 3.7).
- GalK M173L single mutant and M173L-Y371H double mutant were generated using the QuikChange II Site-Directed Mutagenesis Kit from either wild-type or Y371H template, respectively (Stratagene).
- the corresponding mutated plasmids pGalKM173L and pGalKMLYH were constructed by using PfuUltraTM DNA polymerase for mutagenic primer-directed replication from pGalK or pGalKY371H template, respectively, using a pair of mutagenic primers (5′-GTAACTGCGGGATCCTGGATCAGCTAATTTCCG-3′ (SEQ ID NO: 17) and 5′-CGGAAATTAGCTGATCCAGGATCCCGCAGTTAC-3′ (SEQ ID NO: 18)).
- Amplification was accomplished under the following conditions: 5 ⁇ L of 10 ⁇ reaction buffer, 40 ng template DNA, 120 ng of each oligonucleotide primer, 1 ⁇ L dNTPs mixture (2.5 mM), 2.5 U of PfuUltraTM high-fidelity DNA polymerase, in a total volume of 50 ⁇ L ddH2O (thermocycler parameters: initial denaturation, 2 min at 95° C.; amplification, 12 cycles, 0.5 min at 95° C., 1 min at 55° C., 6.5 min at 68° C.; terminal hold, 5 min at 68° C.).
- the amplified plasmids were treated with DpnI to digest the parental DNA template and the mutated prodigy plasmid subsequently used to transform E. coli XL1-blue. The desired point mutation was verified by sequencing.
- GalK mutants Y371H was overexpressed following the procedure previously described for wild-type E. coli GalK, while the overexpression of mutants M173L and M173L-Y371H were accomplished at 16° C. as described for L. lactis GalK.
- the mutant enzymes were purified by using metal affinity chromatography on Ni-NTA Spin Columns (QIAGEN, Valencia, Calif.) and fractions containing homogenous protein were collected, concentrated and quantified using the Bradford protein assay.
- the DNS assay was used to assess the substrate specificity of the purified GalK mutants as previously described. A library of 45 different sugars was screened with each mutant (M173L, Y371H and M173L-Y371H).
- each sugar For each sugar, the DNS assay was used to monitor the reaction progress and control assays in the absence of enzyme or sugar were performed in parallel. Standard curves for each sugar were prepared as described. To assess general percent conversion, each reaction contained 15.0 (M enzyme, 8 mM sugar, 14 mM ATP and 3.5 mM MgCl 2 . The reactions were incubated at 37° C. for 3 hrs after which the reactions were quenched with MeOH, centrifuged (10 min, 12,000 rpm) and then the supernatant (diluted 20-fold) submitted for LC-MS and MS/MS analysis. For monosaccharide kinetic data determination, the sugar concentration was varied over a range of 1-8 mM, under saturating ATP (14 mM).
- Reaction progress was assessed via the DNS assay, wherein a change in absorbance at 575 nm as a function of time was obtained and the initial velocity determined by the slope of the linear phase in the progress curve.
- the kinetic data was analyzed using Enzyme Kinetics Module software (SSPS, Inc., Chicago, Ill.) as previously described.
- the GalK double mutant pGalKMLYH- E. coli was overexpressed at 16° C. via induction of a 40 mL of culture at an OD600 ⁇ 0.7 with IPTG (1 mM). The induced and the cultures were incubated with shaking (140 rpm) for 1 hr and 100 mM 22 or 46 was added to the culture to a final concentration of 4 mM. The cultures were further incubated at 16° C. with shaking (140 rpm) for 16 hr.
- the cells were harvested by centrifugation (15 min, 12,000 rpm) and the recovered cell pellet (380 mg) washed twice with sodium phosphate buffer (20 mL), frozen, thawed and resuspended in H2O:MeOH (1:1). The resuspended solution was heated at 100° C. for 15 min and then sonicated 5 ⁇ 45 sec on ice. Cell debris was collected by centrifugation (15 min, 12,000 rpm) and lyophilized to give the pale white solid (18 mg). To a solution containing all the crude product in 160 ⁇ L of H2O:MeOH (1:1) was added 10 ⁇ mol 50 and 3.2 ⁇ mol of CuI, followed by heating to 50° C. for 24 hours.
- reaction mixture was subsequently centrifuged to remove CuI and the supernatant (diluted 2-fold) directly analyzed by HPLC and LC-MS calculated for C26H37N6O11PS 672.20, found m/z [M+H] ⁇ 671.20.
- Lactococcus lactis GalK potentially allows for a molecular level assessment of this surprising C-4 specificity distinction between the E. coli and L. lactis enzymes.
- L. lactis structure As a template, the sequence alignment of the E. coli and L. lactis GalKs revealed one clear difference among the sugar-binding pockets. Specifically, Leu-182 in L. lactis GalK is near to the C-4 carbon atom of galactose (3.85 ⁇ ) and, based upon sequence alignment, this residue is replaced by Met-173 in E. coli GalK.
- lactis active site predicts the Glc-C-4 equatorial hydroxyl to be 3.79 ⁇ from the ⁇ methyl of Leu 182 ( FIG. 6 a ).
- the identical model in which Leu-182 has been replaced by Met revealed the same Glc-C-4 equatorial hydroxyl to be 1.72 ⁇ from Met sulfur ( FIG. 6 b ).
- Met 173 in E. coli GalK may exclude glucose and thereby limit sugar C-4 specificity to galacto-configured substrates.
- the inventors believe that M-173 in E. coli GalK may also limit C-6 variation.
- FIGS. 7 and 8 illustrate the complete substrate profiles for wild-type E. coli GalK, the E. coli GalK mutant M173L, Y371H and M173L-Y371H.
- the inventor chose the native substrate D-galactose 29, the unique M173L substrate D-glucose 27 and the unique Y371H substrate L-altrose 41 for complete comparative steady-state kinetic profiling.
- D-galactose 29 the native substrate D-galactose 29
- M173L substrate D-glucose 27 the unique M173L substrate D-glucose 27
- Y371H substrate L-altrose 41 for complete comparative steady-state kinetic profiling.
- a slight (around 2-fold) D-galactose Km increase was observed in all three variants (M173L, Y371H and M173L-Y371H).
- 21, 22 and 46 are not a substrates for wild-type E. coli GalK and therefore, the use of a standard E. coli host strain (which contains the inherent wild-type E. coli GalK) should not interfere.
- 21, 22 and 46 each offer a unique functional handle to provide for the rapid installation of a fluorescent label to simplify the chromatographic analysis.
- 22 and 46 are equally reactive to the required fluorescent-labeling via Husigen 1,3-cycloaddition while 21 is poorly reactive ( ⁇ 10% X.
- FIG. 9 b ⁇ 69% conversion of 6-azido-6-deoxy-D-galactose (22) was observed, a slight improvement over the in vitro yield ( ⁇ 50% conversion).
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
One preferred embodiment of the present invention provides a GalK variant comprising a Y371H, M173L or Y371H-M173L mutation for in vivo and in vitro glycorandomization. In another preferred embodiment, the E. coli GalK variant is mutated at one or more amino acids including R28, E34, D37, D174, Y223, C339, Y371, Y371H, M173, and M173L. The GalK variants display catalytic activity toward a variety of D or L sugars. Another preferred embodiment provides method of phosphorylating sugars comprising the step of incubating a nucleotide triphosphate (NTP) and a D or L sugar in the presence of a GalK variant such that a sugar phosphate is produced. This sugar phosphate may be further incubated with a nucleotidylyltransferase, such that a NDP-sugar is produced. The NDP-sugar may be further incubated with a biomolecule capable of being glycosylated in the presence of a glycosyltransferase, such that a glycosylated biomolecule is produced.
Description
- The present application is a divisional of U.S. application Ser. No. 10/904,941, filed Dec. 6, 2004, which claims the benefit of U.S. Provisional 60/481,742, filed Dec. 5, 2003, both of which are incorporated by reference herein.
- The present invention was made by government support from the National Institutes of Health—Grant No. AI52218. The United States government has certain rights in this invention.
- This invention generally relates to sugar kinases and specifically to novel anomeric D/L sugar kinases with expanded substrate specificity and methods of use.
- Many clinically important medicines are derived from glycosylated natural products, the D- or L-sugar substituents of which often dictate their overall biological activity. This paradigm is found throughout the anticancer and antiinfective arenas with representative clinical examples (
FIG. 1 a) including enediynes (calicheamicin, 1), polyketides (doxorubicin, 2; erythromycin, 3), indolocarbazoles (staurosporine, 4), non-ribosomal peptides (vancomycin, 5), polyenes (nystatin, 6), coumarins (novobiocin, 7), or cardiac glycosides (digitoxin, 8). Given the importance of the sugars attached to these and other biologically significant metabolites, extensive effort has been directed in recent years toward altering sugars as a means to enhance or alter natural product-based therapeutics by both in vivo and in vitro approaches. Among these, in vitro glycorandomization (IVG) makes use of the inherent or engineered substrate promiscuity of nucleotidylyltransferases and glycosyltransferases to activate and attach chemically synthesized sugar precursors to various natural product scaffolds. This efficiently incorporates highly functionalized “unnatural” sugar substitutions into the corresponding natural product scaffold (FIG. 1 b). - Accordingly, the need remains for natural and/or engineered enzymes that are promiscuous in their substrate specificity and capable of increased catalytic activity to enhance multiplicity of available glycosylated natural compounds.
- The present invention provides sugar kinases with expanded substrate specificity and methods of use. One embodiment of the present invention provides a GalK variant for in vivo glycorandomization selected from the group consisting of a Y371H, M173L and Y371H-M173L mutations. The GalK variant displays substrate specificity toward a D or L sugar. Preferably, the D or L sugar may be selected from the group consisting of D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose.
- Another embodiment of the present invention provides a method of providing a sugar phosphate. The method comprises the step of incubating a nucleotide triphosphate (NTP) and a D or L sugar in the presence of a GalK variant as discussed in the above paragraph, such that a sugar phosphate is produced. In this method, the NTP is ATP. Also this method may be carried out in a host cell. Further, in this method, the D or L sugar includes galactose or glucose-configured sugars having substitutions at C-2, C-3, C-4, C-6 positions. Preferably, the D or L sugar include D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose.
- Yet another embodiment of the present invention provides an E. coli GalK variant of the wild type amino acid sequence of SEQ ID NO: 1 wherein the wild type amino acid sequence is mutated at one or more amino acid residues. The mutations are selected from the group consisting of R28, E34, D37, D174, Y223, C339, Y371, Y371H, M173, M173L and C353. This variant is capable of displaying catalytic activity toward a D or L sugar selected from the group consisting of D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose. In a preferred embodiment, the GalK variant is Y371H-M173L.
- Another embodiment of the present invention provides a method of phosphorylating sugars. This method comprises the step of incubating a nucleotide triphosphate (NTP) and a D or L sugar in the presence of a GalK variant according as discussed above, such that a sugar phosphate is produced. In this method also, the NTP is ATP. Further, the method is carried out in a host cell. Also the D or L sugar in this method is selected from the group consisting of D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose.
- Yet another aspect of the present invention provides a method of synthesizing an NDP-sugar. This method comprises the steps of: (a) incubating a nucleotide triphosphate (NTP) and a D or L sugar in the presence of a GalK variant as discussed, whereby a sugar phosphate is produced; and (b) incubating the sugar phosphate with a nucleotidylyltransferase, such that a NDP-sugar is produced. In this method, the D or L sugar is selected from the group consisting of D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose. Further, the nucleotidylyltransferase is Ep or a mutated variant thereof. Preferably, the mutated Ep variant includes an Ep mutated at one or more amino acids selected from the group consisting of V173, G147, W224, N112, G175, D111, E162, T201, I200, E199, R195, L89, L89T, L109, Y146 and Y177. In this method also, the NTP is ATP. Also in this method the GalK variant is Y371H, M173L or Y371H-M173L. This method may be carried out in vitro or in a host cell. When the method is carried out in a host cell, the host cell is preferably a bacterium. More preferably, the host cell is selected from the group consisting of E. coli and S. lividans.
- Another aspect of the invention provides a method of producing a glycosylated biomolecule containing at least one sugar moiety. The method comprises the steps of: (a) incubating a nucleotide triphosphate (NTP) and a D or L sugar in the presence of a GalK variant such that a sugar phosphate is produced; (b) incubating the sugar phosphate with a nucleotidylyltransferase, such that a NDP-sugar is produced; and (c) incubating the NDP-sugar with a biomolecule capable of being glycosylated in the presence of a glycosyltransferase, whereby a glycosylated biomolecule is produced. Preferably in this method, the D or L sugar is selected from the group consisting of D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose. Also, preferably, the nucleotidylyltransferase is Ep or a mutated variant thereof. Mutated Ep variant includes Ep that is mutated at one or more amino acids selected from the group consisting of V173, G147, W224, N112, G175, D111, E162, T201, I200, E199, R195, L89, L89T, L109, Y146 and Y177. Further the glycosyltransferase is selected from the group consisting of CalB, CalE, CalN, CalU, Gra orf14, Gra orf5, LanGT1, LanGT2, LanGT3, LanGT4, MtmGI, MtmGII, MtmGTIII, MtmGTIV, NovM, RhlB, Rif
orf 7, SnogD, SnogE, SnogZ, UrdGT1a, UrdGT1b, UrdGT1c, UrdGT2, AknK, AknS, DesVII, DnrS, OleG1, OleG2, TylCV, TylMII, TylN, DauH, DnrH, EryBV, EryCIII, Ngt, BgtA, BgtB, BgtC, GftA, GftB, GftC, GftD, GftE, Gp1-1, Gp1-2, RtfA, AveBI, BlmE, BlmF, MgtA, NysD1, OleD, OleI, SpcF, SpcG, StrH, Ugt51B1, Ugt51C1, UGT52, UgtA, UgtB, UgtC, UgtD and homologs thereof. Also in this method, the NTP is ATP. Preferably, the GalK variant is Y371H, M173L or Y371H-M173L. This method may be carried out in vitro or in a host cell. When the method is carried out in a host cell, preferably, the host cell is a bacterium. More preferably, the host cell is selected from the group consisting of E. coli and S. lividans. Also, in this method the biomolecule capable of being glycosylated is selected from the group consisting of natural and synthetic metabolites, pyran rings, furan rings, enediynes, anthracyclines, angucyclines, aureolic acids, orthosomycins, macrolides, aminoglycosides, non-ribosomal peptides, polyenes, steroids, lipids, indolocarbazoles, bleomycins, amicetins, benzoisochromanequinones coumarins, polyketides, pluramycins, aminoglycosides, oligosaccharides, peptides, proteins, hybrids consisting of one or more these components, analogs and bioactive aglycons thereof. Furthermore, the glycosylated biomolecule is further incubated with at least one chemoselectively ligatable moiety, such that at least one chemoselectively ligated compound is produced. - Various other features, objects, and advantages of the invention will be apparent to those skilled in the art from the following detailed description including illustrative examples setting forth how to make and use the invention.
-
FIG. 1 . a) Provides representative examples for natural product glycosides used as therapeutics: calicheamicin (1), doxorubicin (2), erythromycin (3), staurosporine (4), vancomycin (5), nystatin (6), novobiocin (7), and digitoxin (8). The attached sugars are highlighted in color with red indicating L-configured sugars, and blue representing D-sugars. b) Schematic for natural product in vitro glycorandomization. Ep denotes α-D-glucopyranosyl phosphate thymidylyltransferase, GlyTn different glycosyltransferases. -
FIG. 2 . Provides reactions catalyzed by anomeric kinases. a) Glycogen phosphorylase. b) Fucokinase. c) Galactokinase. d) Proposed phosphorylation of L-altrose accomplished by the evolved GalK mutant Y371H. -
FIG. 3 . Provides representative quantitative data for a set of GalK variants, illustrating screen for D-galacturonic acid (X-axis) and L-altrose (Y-axis). The higher the loss in absorption (shown in absorption units (AU), the more active the enzyme variant. -
FIG. 4 . Provides 3JH-H coupling patterns and NOESY correlations for the GalK Y371H product β-L-altrose-1-phosphate. -
FIG. 5 . Glycorandomization overview and two potential scenarios for an in vivo approach. (a) In vitro glycorandomization utilizes two enhanced enzymes—E1 (a general kinase) and E2 (a general nucleotidylyltransferase)—to generate NDP-sugar substrate libraries to be utilized by a flexible natural product-associated glycosyltransferase (GlyT). (b) In vivo glycorandomization scenario I—feeding monosaccharides to a natural product-producing host engineered to express the ‘NDP-sugar factory’. In this scenario, both the aglycon and glycosyltransferase are provided by the bacterial host. (c) In vivo glycorandomization scenario II—feeding monosaccharides and aglycons to a non-producing host engineered to express the ‘NDP-sugar factory’ and an appropriate glycosyltransferase or glycosyltransferase library. -
FIG. 6 . Comparison of D-glucose docked within the active site of L. lactis GalK and homology-model of E. coli GalK. (a) Wild type L. lactis GalK bound to D-Glc. (b) L182M (the homology model for E. coli GalK active site) with D-Glc. -
FIG. 7 . ‘Natural’ and ‘unnatural’ substrates of wild-type GalK and GalK mutants (M173L, Y371H and M173L-Y371H). -
FIG. 8 .FIG. 8 Percent conversion of sugar substrates by wild-type and mutant GalKs. For each enzymatic reaction: [sugar]=8 mM, [ATP]=14 mM, [MgCl2]=3.5 mM, [enzyme]=15.0 μM and reaction time=180 min. -
FIG. 9 . In vivo GalK-catalyzed sugar-1-phosphate production. (a) Experimental overview using 6-azido-6-deoxy-D-galactose (22) as an example: 22 is fed to an E. coli host expressing the M173L-Y371H GalK double mutant and the reactants and products subsequently labeled using a 1,3-dicycloaddition with thefluorescent tag 50. (b) HPLC chromatographs of bioconversions: i) 22 with Y371H-M173L GalK; ii) 46 with Y371H-M173L GalK; iii) 22 with wild-type GalK. MS: 50, calculated for C20H25N3O3S 387.2, found m/z 386.1 [M−H]−; 51, calculated for C26H37N6O11PS 672.2, found m/z 671.2 [M−H]−; 52, calculated for C26H36N6O8S 592.2, found m/z 591.2 [M−H]− - Sugar phosphates, as the starting material, play a key role in the entire IVG process. Thus, the ability to rapidly construct sugar phosphate libraries would directly contribute to the efficiency of IVG. Compared with the existing chemical synthetic methods for anomeric phosphorylation, single step enzymatic (kinase) routes bypass required multistep synthetic manipulations and could be coupled to IVG in a single reaction vessel. Known C-1 phosphorylating enzymes are limited to mainly three types (
FIG. 2 ): the glycogen phosphorylases which convert glycogen (9) into D-glucose-1-phosphate (10), fucokinases which transfer a phosphate from ATP to the anomeric position of L-fucose (12) to provide β-L-fucose-1-phosphate (13), and the galactokinases (GalK), which catalyze the formation of α-D-galactose-1-phosphate (Gal-1-P, 15) from D-galactose (14) and ATP. Previous studies have revealed GalK from various sources have a limited substrate scope and in all C-1 kinases studied thus far, a strict adherence to either D-sugars (GalK and glycogen phosphorylases), or L-sugars (as in fucokinase) was observed. Thus, in order to apply any of these kinases for generating a randomized sugar phosphate library, their monosaccharide substrate promiscuity must first be enhanced. - Two general routes for altering enzyme substrate specificity are currently available. Structure-based engineering relies upon knowledge of an enzyme's three dimensional structure and an explicit molecular-level understanding of substrate recognition. An example of structure-based engineering as applied to IVG includes increasing the substrate scope of nucleotidylyltransferases employed (
FIG. 1 b, “Ep”). The application of Ep rational engineering led to active site mutants capable of accepting a variety of substrates not utilized by the wild-type enzyme. Such techniques are also described in the U.S. Patent Publications 2003/0055235A1 and 2003/0068669A1, and International Publications WO02079150 and WO0248331, which are incorporated herein by reference in their entirety for all purposes. - The alternative to rational engineering is enzyme evolution, a process primarily dependent upon the availability of a selection or high throughput screen for the desired enhanced or altered enzymatic properties. With respect to carbohydrate enzymology, recent applications include the tagatose-1,6-bisphosphate aldolase modified by in vitro evolution toward an unnatural stereoselectivity, an evolved N-acetylneuraminic acid aldolase for L-sialic acid synthesis, or a 2-deoxy-D-ribose-5-phosphate aldolase with an expanded substrate range after site directed mutagenesis. Usually, in vitro evolution strategies include error-prone PCR for gene diversification, and/or locating critical amino acid residues for saturation mutagenesis or, more prominently, shuffling of fragmented diversified genes or gene families according to a number of different protocols. Subsequently, the diversified proteins are subjected to a screen. In a recent demonstration of IVG, >50 analogs of 5 (vancomycin) were generated, some of which displayed enhanced and distinct antibacterial profiles from the parent natural product.
- While the first structure for a sugar C-1 kinase (GalK from Lactococcus lactis) recently emerged, the extreme variability in solution structures among anticipated monosaccharide library members and the availability of a specific high throughput sugar anomeric kinase calorimetric screen prompted an initial evolutionary approach. As a model system, the inventors selected the well-characterized Escherichia (E.) coli galactokinase GalK and focused the evolutionary approach toward significant C-5 (e.g. L-sugar variants) and C-6 alterations (e.g. deoxy, amino, uronic acid derivatives) in an attempt to probe and elucidate the specific enzymatic architecture responsible for restricting substrate substitution at C-5 and C-6. All variants used herein to describe sugar kinases are defined as unnaturally occurring variants of naturally occurring sugar kinases.
- Herein the inventors describe the application of directed evolution and a high throughput multi-sugar calorimetric screen to enhance the catalytic capabilities of the E. coli GalK. From this approach, one particular GalK mutant carrying a single amino acid exchange (Y371H) displayed a surprisingly substantial degree of kinase activity toward sugars as diverse as D-galacturonic acid, D-talose, L-altrose, and L-glucose, all of which failed as wild-type GalK substrates. Furthermore, this mutant provides enhanced turnover of the small pool of sugars converted by the wild-type enzyme. Comparison of this mutation to the recently solved structure of Lactococcus lactis GalK, begins to provide a blueprint for further engineering of this vital class of enzyme. In addition, the rapid access to such promiscuous sugar C-1 kinases will significantly enhance accessibility to natural and unnatural sugar-1-phosphates and thereby impact upon both in vitro and in vivo glycosylation methodologies such as natural product glycorandomization.
- Generally the present invention provides sugar kinases with expanded substrate specificity and methods of use. One embodiment of the present invention provides a GalK variant for in vivo glycorandomization selected from the group consisting of a Y371H, M173L and Y371H-M173L mutation. The GalK variant displays substrate specificity toward a D or L sugar. Preferably, the D or L sugar may be selected from the group consisting of D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose.
- Another embodiment of the present invention provides a method of providing a sugar phosphate. The method comprises the step of incubating a nucleotide triphosphate (NTP) and a D or L sugar in the presence of a GalK variant according to
claim 1, such that a sugar phosphate is produced. In this method, the NTP is ATP. Also this method may be carried out in a host cell. Further, in this method, the D or L sugar includes galactose or glucose-configured sugars having substitutions at C-2, C-3, C-4, C-6 positions. Preferably, the D or L sugar include D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose. - Yet another embodiment of the present invention provides an E. coli GalK variant of the wild type amino acid sequence of SEQ ID NO: 1 wherein the wild type amino acid sequence is mutated at one or more amino acid residues. The mutations are selected from the group consisting of R28, E34, D37, D174, Y223, C339, Y371, Y371H, M173, M173L and C353. This variant is capable of displaying catalytic activity toward a D or L sugar selected from the group consisting of D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose. In a preferred embodiment, the GalK variant is Y371H-M173L.
- Another embodiment of the present invention provides a method of phosphorylating sugars. This method comprises the step of incubating a nucleotide triphosphate (NTP) and a D or L sugar in the presence of a GalK variant according as discussed above, such that a sugar phosphate is produced. In this method also, the NTP is ATP. Further, the method is carried out in a host cell. Also the D or L sugar in this method is selected from the group consisting of D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose.
- Yet another aspect of the present invention provides a method of synthesizing an NDP-sugar. This method comprises the steps of: (a) incubating a nucleotide triphosphate (NTP) and a D or L sugar in the presence of a GalK variant as discussed, whereby a sugar phosphate is produced; and (b) incubating the sugar phosphate with a nucleotidylyltransferase, such that a NDP-sugar is produced. In this method, the D or L sugar is selected from the group consisting of D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose. Further, the nucleotidylyltransferase is Ep or a mutated variant thereof. Preferably, the mutated Ep variant includes an Ep mutated at one or more amino acids selected from the group consisting of V173, G147, W224, N112, G175, D111, E162, T201, I200, E199, R195, L89, L89T, L109, Y146 and Y177. In this method also, the NTP is ATP. Also in this method the GalK variant is Y371H, M173L or Y371H-M173L. This method may be carried out in vitro or in a host cell. When the method is carried out in a host cell, the host cell is preferably a bacterium. More preferably, the host cell is selected from the group consisting of E. coli and S. lividans.
- Another aspect of the invention provides a method of producing a glycosylated biomolecule containing at least one sugar moiety. The method comprises the steps of: (a) incubating a nucleotide triphosphate (NTP) and a D or L sugar in the presence of a GalK variant such that a sugar phosphate is produced; (b) incubating the sugar phosphate with a nucleotidylyltransferase, such that a NDP-sugar is produced; and (c) incubating the NDP-sugar with a biomolecule capable of being glycosylated in the presence of a glycosyltransferase, whereby a glycosylated biomolecule is produced. Preferably in this method, the D or L sugar is selected from the group consisting of D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose. Also, preferably, the nucleotidylyltransferase is Ep or a mutated variant thereof. Mutated Ep variant includes Ep that is mutated at one or more amino acids selected from the group consisting of V173, G147, W224, N112, G175, D111, E162, T201, I200, E199, R195, L89, L89T, L109, Y146 and Y177. Further the glycosyltransferase is selected from the group consisting of CalB, CalE, CalN, CalU, Gra orf14, Gra orf5, LanGT1, LanGT2, LanGT3, LanGT4, MtmGI, MtmGII, MtmGTIII, MtmGTIV, NovM, RhlB,
R1f orf 7, SnogD, SnogE, SnogZ, UrdGT1a, UrdGT1b, UrdGT1c, UrdGT2, AknK, AknS, DesVII, DnrS, OleG1, OleG2, TylCV, TylMII, TylN, DauH, DnrH, EryBV, EryCIII, Ngt, BgtA, BgtB, BgtC, GftA, GftB, GftC, GftD, GftE, Gp1-1, Gp1-2, RtfA, AveBI, BlmE, BlmF, MgtA, NysD1, OleD, OleI, SpcF, SpcG, StrH, Ugt51B1, Ugt51C1, UGT52, UgtA, UgtB, UgtC, UgtD and homologs thereof. Also in this method, the NTP is ATP. Preferably, the GalK variant is Y371H, M173L or Y371H-M173L. This method may be carried out in vitro or in a host cell. When the method is carried out in a host cell, preferably, the host cell is a bacterium. More preferably, the host cell is selected from the group consisting of E. coli and S. lividans. Also, in this method the biomolecule capable of being glycosylated is selected from the group consisting of natural and synthetic metabolites, pyran rings, furan rings, enediynes, anthracyclines, angucyclines, aureolic acids, orthosomycins, macrolides, aminoglycosides, non-ribosomal peptides, polyenes, steroids, lipids, indolocarbazoles, bleomycins, amicetins, benzoisochromanequinones coumarins, polyketides, pluramycins, aminoglycosides, oligosaccharides, peptides, proteins, hybrids consisting of one or more these components, analogs and bioactive aglycons thereof. Furthermore, the glycosylated biomolecule is further incubated with at least one chemoselectively ligatable moiety, such that at least one chemoselectively ligated compound is produced. - Following examples depict preferred embodiments of the present invention and are for illustrative purposes only. These examples should not be deemed to narrow the scope of the present invention.
- Materials and Methods:
- Materials. E. coli strains XL1-blue and BL21-Gold (DE3) were purchased from Stratagene (La Jolla, Calif.). The template plasmid pGalK has been previously described. Expression vector pET15b was from Novagen (Madison, Wis.). All reagent grade chemicals and enzymes were purchased from Promega (Madison, Wis.), Sigma (St. Louis, Mo.), Fisher/Acros Organics (Hanover Park, Ill.), or Fluka (Milwaukee, Wis.).
- Chemical synthesis of L-idose and D-idose. The syntheses of D- and L-idose followed literature preparations. Gene diversification, library preparation and characterization. For the gene library used, error-prone PCR (epPCR) was accomplished under the following conditions: 25 mM MgCl2, 0.1 mM MnCl2, 0.2 mM (each) dATP and dGTP, 1.0 mM (each) dCTP and dTTP, 500 pg template plasmid pGalK, 40 pmol (each) primers 5″-CTTGGTTATGCGGGTACTGC-3″ (SEQ ID NO: 15) and 5″-TCCCGCGAAATTAATACGAC-3″ (SEQ ID NO: 16), 5U Taq DNA-polymerase in the buffer supplied with the enzyme, in a total volume of 100 μl using the following thermocycle parameters: initial denaturation, 5 min, 94° C.; amplification, 30 cycles, 94° C. for 0.5 min, 54° C. for 0.5 min, 72° C. for 1.5 min; terminal hold, 5 min at 72° C. The amplification products were digested with BamHI/XbaI, purified on an agarose gel (0.8% w/vol), eluted using the QIAquick extraction kit (QIAGEN, Valencia, Calif.), ligated into appropriately digested pET15b, to directly transform E. coli XL1 blue. Plasmids were isolated from randomly picked colonies (˜20 representatives for each library generated) and sequenced on an ABI 310 automatic DNA sequencer (PerkinElmer, Foster City, Calif.). Upon verifying the desired mutation rate, all transformants were pooled, cultured overnight, the collectively recovered plasmids used to transform E. coli BL21-Gold (DE3) and the library processed as described below.
- Bacterial fermentation. E. coli was grown in LB medium, supplemented with ampicillin (100 μL mL-1 final) under standard conditions. For expression of GalK enzyme library members, individual transformants were grown as 1 mL miniature cultures in 96 deep well blocks overnight as seed cultures, then replicated in fresh medium (2%, vol/vol), afterwards added 15% (vol/vol) glycerol, mixed, and stored at 80° C. The replicated cultures were grown to an OD600˜0.7, protein expression was then induced by adding 1 mM IPTG (final), for 2 hours, then harvested by centrifugation (10 min, 3000×g). The cell paste was frozen at 20° C. Upon thawing the harvested expression cultures, the biomass of each well was resuspended in 50 μL NPI-buffer (50 mM NaH2PO4, 300 mM NaCl, pH 8.0) to which was then added 70 μL NPI-buffer supplemented with 1 μL Lysonase (Novagen, Madison, Wis.) for lysis. Cell debris was collected by centrifugation (10 min, 3000×g), and 20 μL of the clear supernatant, containing ˜0.5 μg of the expressed GalK variant on average, was used for each kinase assay.
- Library screening. Enzymatic reactions and assays were set up and read in 96 well format on a Biomek FX automated liquid handling workstation (Beckman Coulter, Fullerton, Calif.) fitted to a Fluostar Optima plate reader (BMG, Durham, N.C.). The in vitro enzymatic reactions and assays followed the protocol published previously, slightly modified for automated liquid handling: 150 μL sugar solution (8 mM final) and 12 μL ATP/Mg2+ solution (20 mM/5 mM final) were mixed, preincubated at 37° C. for 5 min, then 20 μL of the cleared supernatant was added, and the reaction incubated at 37° C. for an additional 2 h. To assay the phosphorylation reactions, 50 μL of the enzymatic reaction were taken at time zero and after the 2 h incubation, mixed with 100 μL of 3,5-dinitrosalicylic acid (DNS) reagent, heated at 100° C. for 5 min, then immediately chilled on ice for 2 min. Assays were run as endpoint measurements following the decrease in absorption (λ=575 nm, λ=758 M-1 cm-1).
- Characterization of GalK Mutants. The GalK mutant Y371H was overexpressed and purified following the procedure previously described for wild-type GalK. The fractions containing homogenous Y371H GalK were collected, concentrated and quantified using the Bradford protein assay. The DNS assay was used to assess the substrate specificity of the purified GalK mutant (Y371H) as previously described. Standard curves for each sugar were prepared as described. In order to determine the kinetic data for each active monosaccharide substrate, the sugar concentration was varied over a range of 1-16 mM, under saturating ATP (15 mM). Using the DNS assay, change in absorbance at 575 nm as a function of time was obtained and the initial velocity determined by the slope of the linear phase in the progress curve. The kinetic data was analyzed using Enzyme Kinetics Module software (SSPS, Inc., Chicago, Ill.) as previously described.
- Preparative phosphorylation of L-altrose and product characterization. L-altrose (21.6 mg, 0.12 mmol) was dissolved in 15
mL 50 mM sodium phosphate buffer (pH 7.5). To this solution, ATP (125 mg, 0.23 mmol), MgCl2.6H2O (15.3 mg, 0.07 mmol) were added, the mixture incubated at 37° C. for 5 min, the reaction initiated via the addition of enzyme (Y371H) to a final concentration of 150 μg mL-1 and reaction progress monitored by TLC. After completion, the mixture was diluted 5-fold in ddH2O and applied to a 200 mL anion exchange column (Q-Sepharose fast flow, Amersham, Piscataway, N.J.). The column was eluted with a gradient of 0-400 mM NaCl and the active fraction was collected and evaporated under reduced pressure. The crude product was desalted on a P-2 gel filtration column (The Nest Group, Southboro, Mass.) to give 16.1 mg of purified product (yield: 52%). [α]D=3.5° (c=1, H2O) 1H NMR (D2O): 5.48 (dd, J=8.6, 1.8 Hz, 1H), 4.14 (dd, 5.3, 3.3 Hz, 1H), 3.99 (m, 1H), 3.98 (dd, J=4.3, 1.8 Hz, 1H), 3.91 (d, J=3.3, 1H), 3.89 (m, 1H), 3.84 (dd, J=12.1, 7.8 Hz, 1H); 13C NMR (D2O): 94.10, 76.23, 70.70, 69.99, 65.68, 62.21; 31P NMR (D2O): 3.77; MS: calculated for C6H13O9P 260.0, found m/z 259.0 [M−H]−. - Results and Discussion:
- Directed evolution of GalK with expanded specificity. The cloned wild-type galK gene from E. coli was subjected to random mutagenesis by epPCR performed over the entire gene. The level of sequence alteration was adjusted to an average of 1.5 amino acid substitutions per enzyme molecule, and verified by DNA-sequencing of corresponding genes. However, epPCR usually results in a more or less strong mutational bias (29, 30, 44) and is therefore not a truly random process. Under the conditions selected, the library contained a transition/transversion ratio of ˜3.0, the transitions outnumbering the transversions, and the AT→GC/GC→AT ratio was found to be 2.4. A population of 3,500 GalK variants from this library were evaluated for their ability to accommodate an expanded spectrum of sugar substrates. The inventors' goals were kinase activity toward L-configured sugars (C-5 alteration) or to those with altered substituents at C-6. Unlike most assays in high-throughput campaigns which screen or select toward a single substrate or substrate mimic, the inventors' recently developed DNS assay is universally applicable to all reducing sugars. Consequently, simultaneous screens for a relaxed enzyme specificity were carried out with a set of appropriately selected sugar substrates rather than a one-dimensional single substrate screen of each library member. To first focus upon C-5 and C-6, the set included a single C-5 challenge—L-altrose (Table 1, 16, the C-5 epimer of D-galactose)—and three C-6 challenges-6-deoxy-D-galactose (22, D-fucose), 6-amino-6-deoxy-D-galactose (23) and D-galacturonic acid (24). Of this set, only D-fucose (22) showed any turnover with wild-type GalK, albeit 2.7% that observed for D-galactose (14). Table 1 describes the kinetic data for the ten active substrates of the GalK variant (Y371H).
-
wild type Y371H sugar substrate Km* kcat † kcatKm ‡ Km* kcat † kcatKm ‡ D-Gal (14) 2.1 108 51.4 5.6 220 39.3 2-deoxy- D-Gal (18) 3.6 30 8.3 4.7 200 42.6 D-GalNH2 (19) 2.9 11.7 4.0 8.8 260 29.5 D-Tal (20) —§ —§ —§ 2.9 45.5 15.7 3-deoxy- D-Gal (21) 6.4 5.1 0.8 10.1 64.6 6.4 6-deoxy- D-Gal (22) 4.9 2.9 0.59 8.0 101 12.6 6-amino- D-Gal (23) —§ —§ —§ 21.3 149 7.0 D- Galacturonicacid (24) —§ —§ —§ 3.2 58 18.1 L-Alt (16) —§ —§ —§ 5.2 80 15.4 L-Glc (25) —§ —§ —§ 2.7 65 24.1 *mM †min−1 ‡mM−1 min−1 §no conversion - The graphical display of a typical screening result is given in
FIG. 3 . Surprisingly, after only one round of evolution two GalK variants appeared independently which phosphorylated the C-5/C-6 set of sugars with roughly similar efficiency. Subsequent DNA-sequencing of both GalK variant genes revealed that they were identical in their sequence and carried a single forward mutation, a C→T transition at position 1111 of the wild-type reading frame, which translates into a tyrosine 371 to histidine replacement. - The structure of the Lactococcus (L.) lactis galactokinase was published. Despite a rather low sequence homology to the E. coli GalK (36% identity, 53% similarity) these two kinases clearly share three characteristic footprint motifs, and all amino acid residues found within the catalytic center of the L. lactis galactokinase (R36, E42, D45, D183, and Y233) are invariably present in its E. coli homolog (R28, E34, D37, D1174, Y223, respectively), embedded in highly conserved sequence environments. For this reason, the inventors speculate that the equivalent residues also form the catalytic apparatus in the E. coli enzyme. Surprisingly, residue 371 in the E. coli wild-type enzyme, found to be essential for the widened substrate specificity and the activity toward L-configured sugars, does not appear as part of its deduced active site. In the L. lactis galactokinase the Cα of the equivalent amino acid Y385 located within the C-terminal domain β-strand K is ˜20 Å from the anomeric carbon of the substrate when bound in the active site. In the L. lactis galactokinase crystal structure Y385 is located in close proximity to C353 (C339 in E. coli GalK). The tyrosine phenolic oxygen is located ˜5.5 Å from the cysteine sulfur atom. Interestingly, during the screen, a mutant on position 339 was also discovered with a similarly expanded substrate profile yet low catalytic activity regardless of the sugar (data not shown). This implicates a potential Y385-C353 (Y371-C339 in E. coli GalK) side chain interaction may play a role in stabilizing this structure and thereby dictate substrate specificity. While an induced fit model has not been previously put forth for this class of enzyme, such “gate-keeping” interactions are known to occur in other enzymes devoted to carbohydrate metabolism, such as hexokinase.
- Characterization of the GalK variant (Y371H). To determine the substrate specificity of the Y371H GalK variant, a sugar library of twenty putative substrates was tested with the purified enzyme. For each sugar, both the DNS assay and thin layer chromatography were used to monitor the reaction progress and control assays in the absence of enzyme or sugar were performed in parallel. The mutant GalK demonstrated the ability to turn over compounds 14, 16, 18-25 (Table 1), strikingly expanding the overall substrate scope compared with wild-type E. coli GalK. The kinetic parameters of the mutant enzyme with all active substrates (14, 16, 18-25) were determined using the DNS assay and compared with wild-type GalK activity. These kinetic studies also revealed, as expected, the evolved enzyme remains an efficient catalyst with D-galactose (kcat=220 min-1, Km=5.6 mM) and displays remarkably enhanced kcat values for all the previously known substrates for wild-type GalK (14, 18, 19, 21, 22), the affinity for which is slightly reduced in all cases.
- While most in vitro evolution projects require repeated rounds of random mutation and/or recombination to generate the desired activity, a leap in GalK catalytic activity and substrate selectivity was accomplished in the initial round of random mutagenesis. Other recent similar examples of single forward mutations leading to a catalytic shift include, for example, the Arabidopsis thaliana cycloartenol synthase or yeast lanosterol synthase, or the adipyl acylase evolved from a Pseudomonas glutaryl acylase. From an analysis of the GalK substrate specificity profiles, one can begin to construct a loose structure-activity requirement for both wild-type enzyme and the corresponding Y371H mutant. Specifically, wild-type GalK displays a stringent requirement for the substrate galactose architecture from C-3 through C-6 and is capable of limited flexibility toward substitution at C-2. Yet, it is interesting to note these stringent requirements, with the exception of the extensive contacts at C-4, are not readily apparent in the L. lactis GalK active site structure. In contrast to wild-type GalK, the Y371H mutation retains primarily only the stringent requirement for the C-4 galactose architecture with an enhanced substrate specificity flexibility at all other positions of the sugar. Remarkably, with essentially all substrates accepted, an enhancement of catalytic efficiency was observed in the Y371H mutant, the enhancement ranging from 5- to 22-fold. The only exception was the wild-type substrate galactose for which the catalytic efficiency was decreased slightly in comparison to wild-type GalK, albeit kcat in this case was also increased 2-fold.
- Confirmation of L-sugar conversion. The substrate specificity studies have demonstrated GalK variant Y371H to be a D/L-unspecific sugar kinase. To confirm the evolved enzyme retains regio- and stereoselectivity with L-sugar substrates, a representative L-sugar reaction product was further characterized. Specifically, a small-scale preparative phosphorylation reaction was performed with L-altrose (21.6 mg, 0.12 mmol). The DNS assay indicated 91% of L-altrose conversion within four hours. Product isolation was readily achieved by anion exchange chromatography, and the yield of purified product was ˜52%. The purified product was characterized by 1H and 13C NMR from which H—H coupling and NOESY data confirmed the product to beβ-L-altrose-1-phosphatein a 1C4 conformation (
FIG. 4 ). In particular, 3JH-H coupling data showed two typical axial-equatorial couplings (H1-H2, H3-H4) and one equatorial-equatorial coupling (H2-H3). NOESY data also revealed the anticipated correlations consistent with this structure (H1-H2, H1-H5, H3-H4, H4-H6 and H5-H6). Based upon this data the inventors propose the Y371H mutant must bind and phosphorylate L-altrose in the same 4C1 conformation as D-galactose (FIG. 2 c and 2 d) (38) the product of which subsequently rapidly equilibrates to the more stable 1C4 conformation upon release from the enzyme. - Implications for In Vitro Glycorandomization:
- Apart from total synthesis, current approaches to alter glycosidic structures include, for example, combinatorial biosynthesis or in vitro biocatalysis. Combinatorial biosynthesis primarily relies on in vivo diversification via genetic engineering of involved sugar biosynthetic pathways. Mendez, C., & Salas, J. (2001) Trends Biotechnol. 11, 449-456. However, combinatorial biosynthesis is significantly limited by enzyme specificity which substantially biases the ultimate extent of diversity accessible. In contrast, IVG presents a significant advantage by providing a truly unbiased library of activated sugars to utilize for drug lead glycosylation. The present advent of kinase-enhanced IVG not only simplifies the upstream availability of sugar-1-phosphates for IVG but also potentially opens the door to in vivo applications of glycorandomization. Specifically, the expression of a tandem promiscuous sugar-1-kinase (GalK) and nucleotidylyltransferase (Ep) in a given organism, presents the prospect of generating a library of NDP-sugars in situ. As such, the present invention provides the foundation for eventually glycorandomizing a variety of clinically important secondary metabolites in vivo to rapidly enhance drug discovery efforts. Such techniques are also described in the U.S. Patent Publications 2003/0055235A1 and 2003/0068669A1, and International Publications WO02079150 and WO0248331, which are incorporated herein by reference in their entirety for all purposes.
- In one embodiment of the present invention, promiscuous sugar-1-kinase (GalK) may be used for synthesizing NDP-sugars. The method of synthesizing comprises the steps of incubating a nucleotide triphosphate (NTP) and a D or L sugar in the presence of a GalK variant such that a sugar phosphate is produced. The sugar phosphate is further incubated with a nucleotidylyltransferase, such that a NDP-sugar is produced. Various anomeric sugars may be used to form the sugar phosphate, including the D or L sugars such as D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose.
- In a preferred embodiment, the nucleotidylyltransferase is Ep or a mutated variant thereof. The mutated Ep variant includes Ep that is mutated at one or more amino acids V173, G147, W224, N112, G175, D111, E162, T201, I200, E199, R195, L89, L89T, L109, Y146 and Y177. In one preferred embodiment the method may be carried out in vitro. In another preferred embodiment, the method is carried out in a host cell. The host cell may be a bacterium. Further, the host cell may be selected from the group consisting of E. coli and S. lividans.
- Another preferred embodiment of the present invention provides a method of producing a glycosylated biomolecule containing at least one sugar moiety. The method comprises the steps of incubating a nucleotide triphosphate (NTP) and a D or L sugar in the presence of a GalK variant, whereby a sugar phosphate is produced; incubating the sugar phosphate with a nucleotidylyltransferase, whereby a NDP-sugar is produced; and incubating the NDP-sugar with a biomolecule capable of being glycosylated in the presence of a glycosyltransferase, such that a glycosylated biomolecule is produced. In a preferred embodiment, the glycosyltransferase is selected from the group consisting of CalB, CalE, CalN, CalU, Gra orf14, Gra orf5, LanGT1, LanGT2, LanGT3, LanGT4, MtmGI, MtmGII, MtmGTIII, MtmGTIV, NovM, RhlB,
Rif orf 7, SnogD, SnogE, SnogZ, UrdGT1a, UrdGT1b, UrdGT1c, UrdGT2, AknK, AknS, DesVII, DnrS, OleG1, OleG2, TylCV, TylMII, TylN, DauH, DnrH, EryBV, EryCIII, Ngt, BgtA, BgtB, BgtC, GftA, GftB, GftC, GftD, GftE, Gp1-1, Gp1-2, RtfA, AveBI, BlmE, BlmF, MgtA, NysD1, OleD, OleI, SpcF, SpcG, StrH, Ugt51B1, Ugt51C1, UGT52, UgtA, UgtB, UgtC, UgtD and homologs thereof. In another preferred embodiment, the biomolecule capable of being glycosylated is selected from the group consisting of natural and synthetic metabolites, pyran rings, furan rings, enediynes, anthracyclines, angucyclines, aureolic acids, orthosomycins, macrolides, aminoglycosides, non-ribosomal peptides, polyenes, steroids, lipids, indolocarbazoles, bleomycins, amicetins, benzoisochromanequinones coumarins, polyketides, pluramycins, aminoglycosides, oligosaccharides, peptides, proteins, hybrids consisting of one or more these components, analogs and bioactive aglycons thereof. In yet another preferred embodiment, the glycosylated biomolecule is further incubated with at least one chemoselectively ligatable moiety, such that at least one chemoselectively ligated compound is produced. Exempletive chemoligation techniques are described in the United States Patent Application No. (NOT ASSIGNED) “Glycorandomization and Production of Novel Vancomycin Analogs”, filed on Sep. 24, 2003, which is incorporated in its entirety by reference for all purposes. -
TABLE 2 D-Gal (29) D-Gal (29) D-Glc (27) D-Glc (27) L-Alt (21) L-Alt (21) Km Vmax Km Vmax Km Vmax mM mM min − 1 Mm mM min − 1 mM mM min − 1 WT 2.1 (±0.4) 1.5 (±0.5) — — — — GalK M173L 5.9 (±0.8) 1.9 (±0.6) 2.6 (±0.5) 0.02 (±0.01) — — Y371H 5.6 (±0.3) 2.2 (±0.6) — — 6.0 (±0.9) 0.16 (±0.04) M173L- 4.6 (±0.7) 2.3 (±0.4) 4.0 (±0.6) 0.02 (±0.01) 6.3 (±1.1) 0.07 (±0.03) Y371H - Glycorandomization (
FIG. 5 a), a process centered upon the inherent promiscuity of secondary metabolite-associated glycosyltransferases, is one of the latest promising developments toward this important goal. Critical to the success of glycorandomization has been the ability to engineer and/or evolve two additional promiscuous enzymes—anomeric kinases and nucleotidylyltransferases. Taken together with the many elegant methods to synthesize monosaccharide libraries and the intrinsic substrate flexibility of many secondary metabolite-associated glycosyltransferases, this two-enzyme short activation pathway allows one to rapidly diversify the sugars attached to complex natural products. The glycorandomization process is further enhanced via a final diversification step which relies upon the use of downstream chemoselective ligation. - Cumulatively, the successful demonstration of in vitro glycorandomization, the observations that functional NDP-sugar pathways can be reassembled in prokaryotes, and the fact that natural and ‘unnatural’ endogenous sugars are processed in vivo by both prokaryotes and eukaryotes, present the foundation from which to approach in vivo glycorandomization. For example, the expression of a tandem promiscuous sugar-1-kinase (GalK) and nucleotidylyltransferase (Ep)—essentially an unnatural NDP-sugar factory—in a natural aglycon-producing host (e.g. the erythromycin-producing Saccharopolyspora) should present the prospect of generating a glycorandomized library in situ, the glycorandomized metabolite output of which is controlled by monosaccharides being fed to the strain (
FIG. 5 b). Alternatively, expression of the tandem two gene ‘NDP-sugar factory’ genes in a non-producing host (e.g. S. lividans or E. coli) which expresses a given glycosyltransferase (or glycosyltransferase library), should also provide a vehicle to accomplish glycorandomization via feeding the host with appropriate aglycon acceptors and unnatural sugar donors (FIG. 5 c). The key to either in vivo scenario is the ability of unnatural sugars to enter the host and serve as efficient substrates of the first enzyme of the short activation pathway (the flexible anomeric kinase). Toward this goal, a kinase able to process sugars bearing unique mass signatures and/or reactive handles would, in addition to further enhancing library diversification, greatly simplify the final analysis of in vivo access and activity. - The inventor has applied directed enzyme evolution and relied upon a high throughput galactokinase (GalK) assay (DNS assay) for the screening of diverse E. coli GalK variant libraries generated via error-prone PCR. From this approach, one particular GalK mutant (Y371H) demonstrated remarkably widened substrate flexibility toward C-2, C-3 and C-5 substitutions of D-galactose. Yet, the mutant retained a stringent requirement for the axial C-4 galactose architecture. The recently solved L. lactis GalK crystal structure suggested two highly conserved active-site residues (Asp-37 and Tyr-223 in E. coli GalK) are responsible for hydrogen-bonding with this C-4 axial hydroxyl group of the substrate. Yet, saturation mutagenesis at these two critical positions in the E. coli enzyme failed to provide mutants with enhanced C-4 sugar flexibility while a parallel study revealed the L. lactis wild-type GalK and Y385H orthologs to surprisingly display weak activity toward the C-4 epimer, glucose.
- Herein the inventor reports a structure-activity model, based upon the L. lactis active site and its ability to weakly utilize glucose, led to a specific engineered
E. coli M 173L mutant GalK with enhanced C-4 and C-6 promiscuity. Moreover, a combination of the favorable structure-based (M173L) mutation with the beneficial mutation previously discovered via directed evolution (Y371H) drastically exceeds an additive enhancement for both C-4 and C-6 substitutions. Most importantly, the additional unnatural sugar substrates accessed by this unique double mutant allowed the unique opportunity to assess whether unnatural sugars can enter a bacterial host and serve as efficient substrates of the first enzyme of the glycorandomization pathway (the flexible anomeric kinase). Specifically, feeding of the unique substrate 6-azido-6-deoxy-galactose (22) or 6-azido-6-deoxy-glucose (46) to an E. coli host engineered to express M173L-Y371H-GalK followed by the rapid fluorescent labeling of substrates and products viaHuisgen 1,3-dipolar cycloaddition revealed the desired efficient sugar-1-phosphate production in vivo. This result stands as a key first step in demonstrating the concept of in vivo glycorandomization. - Materials and Methods:
- Materials. The syntheses of 4-azido-4-deoxy-D-galactose (21), 6-azido-6-deoxy-D-galactose (22), 6-chloro-6-deoxy-D-galactose (23), 6-bromo-6-deoxy-D-galactose (24), 4-deoxy-D-galactose (25), 6-hydroxymethylene-D-galactose (32), 3-deoxy-D-galactose (34), 6-amino-6-deoxy-D-galactose (35), 6-deoxy-6,6-difluoro-D-galactose (40), were reported previously while other monosaccharide compounds 26-31, 33, 36-39, 41, 42, 45, 47 and 48 were purchased from Sigma (St. Louis, Mo.), Fisher/Acros Organics (Hanover Park, Ill.), or Fluka (Milwaukee, Wis.). E. coli strains XL1-blue and BL21 (DE3) were purchased from Stratagene (La Jolla, Calif.). Expression vector pET15b was purchased from Novagen (Madison, Wis.). Enzymes were purchased from Promega (Madison, Wis.).
- Chemical Synthesis. For chemical synthesis of 6-thio-6-deoxy-D-
galactose 43, 6-thio-6-deoxy-D-glucose 44, 6-azido-6-deoxy-D-glucose 46, and 5-dimethylaminonaphthalene-1-(N-(5-propargylamidepentyl))-sulfonamide 50, see supporting methods, which is published as supporting information on the PNAS web site. - Structure Modeling. The PDB file for the crystal structure of L. lactis wild type GalK was obtained from the Protein Data Bank, Research Collaboratory for Structural Bioinformatics, Rutgers University, New Brunswick, N.J. (available on the Rutgers University Website). The structure modeling was accomplished using Swiss-PdbViewer software (Version 3.7).
- Site-specific Mutagenesis. The GalK M173L single mutant and M173L-Y371H double mutant were generated using the QuikChange II Site-Directed Mutagenesis Kit from either wild-type or Y371H template, respectively (Stratagene). The corresponding mutated plasmids pGalKM173L and pGalKMLYH were constructed by using PfuUltra™ DNA polymerase for mutagenic primer-directed replication from pGalK or pGalKY371H template, respectively, using a pair of mutagenic primers (5′-GTAACTGCGGGATCCTGGATCAGCTAATTTCCG-3′ (SEQ ID NO: 17) and 5′-CGGAAATTAGCTGATCCAGGATCCCGCAGTTAC-3′ (SEQ ID NO: 18)). Amplification was accomplished under the following conditions: 5 μL of 10× reaction buffer, 40 ng template DNA, 120 ng of each oligonucleotide primer, 1 μL dNTPs mixture (2.5 mM), 2.5 U of PfuUltra™ high-fidelity DNA polymerase, in a total volume of 50 μL ddH2O (thermocycler parameters: initial denaturation, 2 min at 95° C.; amplification, 12 cycles, 0.5 min at 95° C., 1 min at 55° C., 6.5 min at 68° C.; terminal hold, 5 min at 68° C.). The amplified plasmids were treated with DpnI to digest the parental DNA template and the mutated prodigy plasmid subsequently used to transform E. coli XL1-blue. The desired point mutation was verified by sequencing.
- Characterization of GalK Mutants. The GalK mutants Y371H was overexpressed following the procedure previously described for wild-type E. coli GalK, while the overexpression of mutants M173L and M173L-Y371H were accomplished at 16° C. as described for L. lactis GalK. The mutant enzymes were purified by using metal affinity chromatography on Ni-NTA Spin Columns (QIAGEN, Valencia, Calif.) and fractions containing homogenous protein were collected, concentrated and quantified using the Bradford protein assay. The DNS assay was used to assess the substrate specificity of the purified GalK mutants as previously described. A library of 45 different sugars was screened with each mutant (M173L, Y371H and M173L-Y371H). For each sugar, the DNS assay was used to monitor the reaction progress and control assays in the absence of enzyme or sugar were performed in parallel. Standard curves for each sugar were prepared as described. To assess general percent conversion, each reaction contained 15.0 (M enzyme, 8 mM sugar, 14 mM ATP and 3.5 mM MgCl2. The reactions were incubated at 37° C. for 3 hrs after which the reactions were quenched with MeOH, centrifuged (10 min, 12,000 rpm) and then the supernatant (diluted 20-fold) submitted for LC-MS and MS/MS analysis. For monosaccharide kinetic data determination, the sugar concentration was varied over a range of 1-8 mM, under saturating ATP (14 mM). Reaction progress was assessed via the DNS assay, wherein a change in absorbance at 575 nm as a function of time was obtained and the initial velocity determined by the slope of the linear phase in the progress curve. The kinetic data was analyzed using Enzyme Kinetics Module software (SSPS, Inc., Chicago, Ill.) as previously described.
- In Vivo Conversion Analysis. The GalK double mutant pGalKMLYH-E. coli was overexpressed at 16° C. via induction of a 40 mL of culture at an OD600˜0.7 with IPTG (1 mM). The induced and the cultures were incubated with shaking (140 rpm) for 1 hr and 100
mM - Results and Discussion:
- Structure-basis for Engineering GalKs with Expanded Specificity. Prior to any available GalK structural information, the directed evolution of E. coli GalK presented a general sugar anomeric kinase with widened flexibility primarily at C-5 and C-6 of the sugar substrate. Interestingly, all C-4-modified derivatives tested in this study failed as substrates for the evolved catalyst. In contrast, the recent analysis of the L. lactis wild-type GalK revealed in vitro conversion of various C-4-modified analogs, including 4-azido-4-deoxy-D-galactose (21), 4-deoxy-D-galactose (25) and D-glucose (27). The recent structure elucidation of Lactococcus lactis GalK potentially allows for a molecular level assessment of this surprising C-4 specificity distinction between the E. coli and L. lactis enzymes. With the L. lactis structure as a template, the sequence alignment of the E. coli and L. lactis GalKs revealed one clear difference among the sugar-binding pockets. Specifically, Leu-182 in L. lactis GalK is near to the C-4 carbon atom of galactose (3.85 Å) and, based upon sequence alignment, this residue is replaced by Met-173 in E. coli GalK. A model of the C-4 epimer of galactose (D-glucose) within the L. lactis active site predicts the Glc-C-4 equatorial hydroxyl to be 3.79 Å from the γ methyl of Leu 182 (
FIG. 6 a). However, the identical model in which Leu-182 has been replaced by Met (to mimic the E. coli GalK active site) revealed the same Glc-C-4 equatorial hydroxyl to be 1.72 Å from Met sulfur (FIG. 6 b). Thus, this model clearly suggests Met 173 in E. coli GalK may exclude glucose and thereby limit sugar C-4 specificity to galacto-configured substrates. Moreover, given the close proximity of the sugar C-6 hydroxyl to Met in this structural model (2.85 Å) (FIG. 6 b), the inventors believe that M-173 in E. coli GalK may also limit C-6 variation. - Characterization of Engineered E. coli GalK Mutants. To test the above hypothesis, a single E. coli GalK M173L mutant was generated and screened against a
panel 45 potential sugar substrates. As predicted, the E. coli M173L engineered mutant displayed moderate D-glucose 27 activity (20% conversion in 3 hr). Moreover, three additional D-gluco-configuredstructures FIGS. 7 and 8 ), which were not substrates of wild-type E. coli GalK (or the evolved E. coli Y371H mutant), were also substrates of the new structure-based variant. While these studies clearly revealed the structure-based M173L mutant to accept a substrate set distinct to that of the previously evolved Y371H mutant, in contrast to the structural model described above, both mutants failed in the presence of substrates presenting even moderate C-6 bulk such as 6-azido-6-deoxy-galactose 22 or 6-azido-6-deoxy-D-glucose 46. In an attempt to further generalize the sugar kinase activity, an E. coli double M173L-Y371H mutant was examined. Remarkably, not only did this double mutant retain the activity of both corresponding single mutants, but this prodigy demonstrated a substantial degree of kinase activity toward a variety of new structures (21-26, 43-48). Most of the new substrates share modifications at C-4 and/or C-6, with many of D-gluco origin. It is also noteworthy that three among this new substrate set are azidosugars (21, 22 and 46), thereby setting the stage for rapid analysis of in vivo bioconversion via post-bioconversion labeling of substrates and products with a fluorescenttag using Huisgen 1,3-cycloaddition.FIGS. 7 and 8 illustrate the complete substrate profiles for wild-type E. coli GalK, the E. coli GalK mutant M173L, Y371H and M173L-Y371H. - To better understand the distinct role of the two particular amino acid residues (Met-173 and Tyr-371) in determining the substrate specificity, the inventor chose the native substrate D-
galactose 29, the unique M173L substrate D-glucose 27 and the unique Y371H substrate L-altrose 41 for complete comparative steady-state kinetic profiling. In comparison to wild-type E. coli GalK, a slight (around 2-fold) D-galactose Km increase was observed in all three variants (M173L, Y371H and M173L-Y371H). Moreover, a comparison of the D-glucose kinetic parameters for M173L and the L-altrose kinetic values for Y371H to those of the double mutant revealed very little change. Thus, in contrast to the notable gain of function (in terms of the shear number of new M173L-Y371H substrates) illustrated inFIG. 7 , the kinetic analysis only predicts the gain of function to be additive at best. In other words, the kinetic analysis predicts the double mutant should accept only known M173L and Y371H substrates but would not predict an expansion beyond this dual substrate set. Yet, while it is difficult to explain this remarkable gain of function in the M173L-Y371H variant, the unique ability of this double mutant to acceptcompounds - In Vivo Bioconversion of Unnatural Sugars Using an Engineered GalK. In the context of assessing in vivo bioconversion, the specific M173L-Y371H-22 relationship is advantageous for two reasons. First, as described above, 21, 22 and 46 are not a substrates for wild-type E. coli GalK and therefore, the use of a standard E. coli host strain (which contains the inherent wild-type E. coli GalK) should not interfere. Second, as previously mentioned, 21, 22 and 46 each offer a unique functional handle to provide for the rapid installation of a fluorescent label to simplify the chromatographic analysis. In this context, 22 and 46 are equally reactive to the required fluorescent-labeling via
Husigen 1,3-cycloaddition while 21 is poorly reactive (<10% X. Fu, unpublished). Thus, for the current in vivo analysis, 21 was excluded. The set selected (22 and 46) still offer the opportunity to test a range of substrates with distinctly unique in vitro profiles. Specifically, 22 is known to have >50% in vitro in 2 hrs while 46 shows ˜15% conversion under the same conditions. - To assess the Y371H-M173L GalK-catalyzed in vivo production of unnatural sugar-1-phosphates (
FIG. 9 a), the unnatural sugars (22 or 46, 4 mM final concentration) were fed to an E. coli host (40 mL culture) which overexpressed the promiscuous GalK. After a designated time, the extracts were analyzed via the specific attachment of a fluorescent tag (50), to both starting material (22 or 46) and desired sugar-1-phosphate products, using 1,3-dipolar cycloaddition. Two controls were run in parallel. The first utilized a strain containing an empty expression vector (pET-15b—the vector used for overexpression of the GalK mutants) while the second employed a wild-type GalK overexpression strain. The crude products from each bioconversion were isolated, labelled via 1,3-dipolar cycloaddition and analyzed by fluorescence HPLC and LC-MS. As illustrated inFIG. 9 b, ˜69% conversion of 6-azido-6-deoxy-D-galactose (22) was observed, a slight improvement over the in vitro yield (˜50% conversion). In a similar manner, 15% conversion of 6-azido-6-deoxy-D-glucose (46) was observed, consistent with the in vitro yield (˜50% conversion). Notably, this success illustrates that unnatural sugars are able to enter the heterologous E. coli host and access the engineered promiscuous sugar kinase. Given this key result, it is likely that the addition of a flexible nucleotidylytransferase (E2) and glycosyltransferase to this host will allow for in vivo glycorandomization. - Implications for In Vivo Glycorandomization:
- The recent developed chemoenzymatic approach in vitro glycorandomization significantly contributes to the diversity of novel therapeutics via altering glycosylation patterns on secondary metabolites. However, the general application of this approach is significantly limited by the two primary issues. First, expensive substrates/cofactors significantly hamper the scaling up the process, although the alternative solutions for regenerating these reagents are available. Second, the application of IVG to most, or all, classes of glycosylated natural products is heavily dependent upon the expression of appropriate glycosyltransferases and establishing in vitro conditions for an active enzyme which, in some cases, can be severely dictated by the solubility of the aglycon acceptor. The present advent of kinase-enhanced IVG potentially opens the door to in vivo applications of glycorandomization, and the inventor believes the in vivo process would be able to overcome these limitations. The current result illustrates the entry of unnatural sugars and their subsequent utilization by the engineered GalK. This result clearly stands as strong evidence supporting the overall feasibility of in vivo glycorandomization. As such, this work provides the foundation for the eventual glycorandomization a variety of clinically important secondary metabolites in vivo to rapidly enhance drug discovery efforts.
- The sugar kinase with expanded substrate specificity useful for glycorandomization of the present invention has many other applications aside from those described in the preferred embodiment and examples. Thus, although the invention has been herein shown and described in what is perceived to be the most certain embodiments, it is to be understood that the invention is not intended to be limited to the specific embodiments set forth above. Rather, it is recognized that certain modifications, substitutions, alterations, omissions may be made by one of skill in the art of the invention without departing from the spirit or intent of the invention. Accordingly, the invention is to be taken as including all reasonable equivalents to the subject matter of the appended claims and the foregoing description is meant to be exemplary only and should not limit the scope of the invention set forth in the following claims.
- All references are incorporated by reference herein for all purposes.
-
- (1) Weymouth-Wilson, A. C. (1997) Nat. Prod. Rep. 14, 99-110;
- (2) Potier, P. (1999), Actual. Chim. 11, 9-11.
- (3) Thorson, J. S., & Vogt, T. in Glycosylated Natural Products in Carbohydrate-based Drug Discovery, 2003, ed: C.-W. Wong (Wiley-VCH Weinheim, Germany) Vol. II, pp 685-711.
- (4) Kren, V., & Martinkova, L. (2001) Curr. Med. Chem. 8, 1303-1328.
- (5) Kren, V. (2001) in Glycoscience: Chemistry and Chemical Biology I-III, eds: Fraser-Reid, B. O., Tatsuta, K., & Thiem, J. (Springer, Heidelberg, Germany), pp 2471-2529.
- (6) Thorson, J. S., Hosted Jr., T. J., Jiang, J., Biggins, J. B., & Ahlert, J. (2001) Curr. Org. Chem. 5, 139-167.
- (7) Albermann, C., Soriano, A., Jiang, J., Vollmer, H., Biggins, J. B., Barton, W. A., Lesniak, J., Nikolov, D. B., & Thorson, J. S. (2003) Org. Lett. 5, 933-936.
- (8) Mendez, C. Biotechnol. 11, 449-456.
- (9) Barton, W. A., Biggins, J. B., Jiang, J., Thorson, J. S., & Nikolov, D. B. (2002) Proc. Natl. Acad. Sci. USA 99, 13397-13402.
- (10) Barton, W. A., Biggins, J. B., Lesniak, J., Jeffrey, P. D., Jiang, J., Rajashankar, K. R., Thorson, J. S., & Nikolov, D. B. (2001) Nat. Struct. Biol. 8, 545-551.
- (11) Thorson, J. S., Barton, W. A., Hoffmeister, D., Albermann, C., & Nikolov, D. B. (2003) ChemBioChem, 5:16-25.
- (12) Jiang, J., Albermann, C., & Thorson, J. S. (2003) ChemBioChem. 4, 443-446;
- (13) Fu, X., Albermann, C., Jiang, J., Liao, J., Zhang, C., & Thorson, J. S. (2003) Nat. Biotech. 21: 1467-1469.
- (14) Jiang, J., Biggins, J. B., & Thorson, J. S. (2001) Angew. Chem. Intl. Ed. 40, 1502-1505.
- (15) Jiang, J., Biggins, J. B., & Thorson, J. S. (2000) J. Am. Chem. Soc. 122, 6803-6804.
- (16) Johnson, L. N., & Barford, D. (1990) J. Biol. Chem. 265, 2409-2412;
- (17) Park, S. H., Pastuszak, I., Drake, R., & Elbein, A. D. (1998) J. Biol. Chem. 273, 5685-5691.
- (18) Lavine, J. E., Cantlay, E., Roberts Jr., C. T., & Morse, D. E. (1982) Biochim. Biophys. Acta 717, 76-85.
- (19) Dey, P. M. (1983) Eur. J. Biochem. 136, 155-159.
- (20) Thomas, P., Bessell, E. M., & Westwood, J. H. (1974) Biochem. J. 139, 661-664.
- (21) Yang, J., Fu, X., Jia, Q., Shen, J., Biggins, J. B., Jiang, J., Zhao, J., Schmidt, J. J., Wang, P. G. & Thorson, J. S. (2003) Org. Lett. 5, 2223-2226.
- (22) Bornscheuer, U. T., & Pohl, M. (2001) Curr. Opin. Chem. Biol. 5, 137-143.
- (23) Petrounia, I. P., & Arnold, F. H. (2000) Curr. Opin. Biotechnol. 11, 325-330.
- (24) Tao, H., & Cornish, V. W. (2002) Curr. Opin. Chem. Biol. 6, 858-864.
- (25) Williams, G. J., Domann, S., Nelson, A., & Berry, A. (2003) Proc. Natl.
Acad. Sci. USA 100, 3143-3148. - (26) Wada, M., Hsu, C. C., Franke, D., Mitchell, M., Heine, A., Wilson, I., & Wong, C.-H. (2003) Bioorg. Med. Chem. 11, 2091-2098.
- (27) DeSantis, G., Liu, J., Clark, D. P., Heine, A., Wilson, I. A., & Wong, C.-H. (2003) Bioorg. Med. Chem. 11, 43-52.
- (28) Leung, D. W., Chen, E., & Goeddel, D. V. (1989)
Technique 1, 11-15. - (29) Cadwell, R. G, & Joyce, G. F. (1992) PCR Meth. Appl. 2, 28-33.
- (30) Liebeton, K., Zonta, A., Schimossek, K., Nardini, M., Lang, D., Dijkstra, B. W. Reetz, M. T. & Jaeger, K.-E. (2000) Chem. Biol. 7, 709-718.
- (31) Stemmer, W. P. C. (1994) Nature 370, 389-391.
- (32) Zhao, H., Giver, L., Shao, Z., Affholter, J. A. & Arnold, F. H. (1998) Nat. Biotech. 16, 258-261.
- (33) Kikuchi, M., Ohnishi, K., & Harayama, S. (1999) Gene 236, 159-167.
- (34) Coco, W. M., Levinson, W. E., Crist, M. J., Hektor, H. J., Darzins, A., Pienkos, P. T., Squires, C. H., & Monticello, D. J. (2001) Nat. Biotech. 19, 354-359.
- (35) Miyazaki, K. (2002) Nuc. Acids Res. 30, e139.
- (36) Zha, D., Eipper, A. & Reetz, M. T. (2003)
ChemBiochem 4, 34-39. - (37) Thoden, J. B. & Holden, H. M. (2003) J. Biol. Chem. 278 33305-33311.
- (38) Debouck, C., Riccio, A., Schumperli, D., McKenney, K., Jeffers, J., Hughes, C., Rosenberg, M., Heusterspreute, M., Brunel, F., & Davison, J. (1985) Nuc. Acids Res. 13, 1841-1853.
- (39) Blanc-Muesser, M., Defaye, J., Horton, D., & Tsai, J.-H. (1980) in Methods in Carbohydrate Chemistry Vol. VIII, eds: Whistler, R. L. & BeMiller, J. N. (Academic Press, Inc., New York), pp 177-183.
- (40) Paulsen, H., Trautwein, W.-P., Espinosa, F. G., & Heyns, K. (1967) Chem. Ber. 100, 2822-2836.
- (41) Paulsen, H., & Herold, C. P. (1970) Chem. Ber. 103, 2450-2462.
- (42) Bradford, M. (1976) Anal. Biochem. 72, 248-254.
- (43) Fromant, M., Blanquet, S., & Plateau, P. (1995) Analyt. Biochem. 224, 347-353.
- (44) Bork, P., Sander, C., & Valencia, A. (1993) Protein Sci. 2, 31-40.
- (45) Aleshin, A. E., Zeng, C., Bourenkov, G. P., Bartunik, H. D., Fromm, H. J., & Honzatko, R. B. (1998)
Structure 6, 39-50. - (46) Segura, M. J. R., Lodeiro, S., Meyer, M. M., Patel, A. J., & Matsuda, S. P. (2002) Org. Lett. 4, 4459-4462.
- (47) Joubert, B. M., Hua, L., & Matsuda, S. P. (2000) Org. Lett. 2, 339-341.
- (48) Herrera, J. B., Wilson, W. K., & Matsuda, S. P. (2000) J. Am. Chem. Soc. 122, 6765-6766.
- (49) Segura, M. J., Jackson, B. E., & Matsuda, S. P. (2003) Nat. Prod. Rep. 20, 304-317.
- (50) Otten, L. G., Sio, C. F., Vrielink, J., Cool, R. H., & Quax, W. J. (2002) J. Biol. Chem. 277, 42121-42127.
- (51) Hoffmeister, D., Yang, J., Liu, L., Thorson, J. S. (2003) Proc. Natl.
Acad. Sci. USA 100, 13184-13189. - (52) Hoffmeister, D., Thorson, J. S. (2004)
ChemBioChem 5, 989-992. - (53) Yang, J.; Liu, L.; Thorson, J. S. (2004)
ChemBioChem 5, 992-996. - (54) Northrup, A. B., MacMillan, D. W. (2004) Science. 305, 1752-1755.
- (55) Northrup A. B., Mangion, I. K., Hettche, F., MacMillan, D. W. (2004) Angew Chem Int Ed Engl. 43, 2152-2154.
- (56) Yang, J., Hoffmeister, D., Liu, L., Fu, X., Thorson, J. S. (2004) Bioorg. Med. Chem. 12, 1577-1584.
- (57) Langenhan, J. M., Thorson, J. S. (2004) Curr. Org. Syn. manuscript in press.
- (58) Zhang, J., Kowal, P., Chen, X., Wang, P. G. (2003) Org. Biomol. Chem. 1, 3048-3053.
- (59) Zhang, J., Chen, X., Shao, J., Liu, Z., Kowal, P., Lu, Y., Wang, P. G. (2003) Methods Enzymol. 362, 106-124.
- (60) Luchansky, S. J., Hang, H. C., Saxon, E., Grunwell, J. R., Yu, C., Dube, D. H., Bertozzi, C. R. (2003) Methods Enzymol. 362, 249-272.
- (61) Fuster, M. M., Brown, J. R., Wang, L., Esko, J. D. (2003) Cancer Res. 63, 2775-2781.
- (62) Mong, T. K., Lee, L. V., Brown, J. R., Esko, J. D., Wong, C. H. (2003) Chembiochem. 4, 835-840.
- (63) Shao, J., Hayashi T., Wang P. G. (2003) Appl. Environ. Microbiol. 69, 5238-5342.
- (64) Thoden, J. B., & Holden, H. M. (2003) J. Biol. Chem. 278, 33305-33311.
- (65) Bradford, M. (1976) Anal. Biochem. 72, 248-254.
Claims (32)
1. A method of providing a sugar phosphate comprising the step of incubating a nucleotide triphosphate (NTP) and a D or L sugar in the presence of a GalK variant of the wild type E. Coli GalK amino acid sequence set forth in SEQ ID NO: 1, wherein the variant has one or both of the following mutations: tyrosine 371 is mutated to histidine (Y371H), and/or (b) methionine 173 is mutated to leucine (M173L), and whereby a sugar phosphate is produced.
2. The method of claim 1 , wherein the NTP is ATP.
3. The method of claim 1 , wherein said method is carried out in a host cell.
4. The method of claim 1 , wherein the D or L sugar is galactose or glucose-configured sugars having substitutions at C-2, C-3, C-4, C-6 positions.
5. The method of claim 1 , wherein the sugar is selected from the group consisting of D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose.
6. A method of phosphorylating sugars comprising the step of incubating a nucleotide triphosphate (NTP) and a D or L sugar in the presence of a GalK variant of the wild type E. coli GalK amino acid sequence set forth in SEQ ID NO: 1, wherein the variant has one or more mutations selected from the group consisting of R28, E34, D37, D174, Y223, C339, Y371, and M173, whereby a sugar phosphate is produced.
7. The method of claim 6 , wherein NTP is ATP.
8. The method of claim 6 , wherein said method is carried out in a host cell.
9. The method of claim 6 , wherein the D or L sugar is selected from the group consisting of D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose.
10. A method of synthesizing an NDP-sugar, comprising the steps of:
(a) incubating a nucleotide triphosphate (NTP) and a D or L sugar in the presence of a GalK variant of the wild type E. coli GalK amino acid sequence set forth in SEQ ID NO: 1, wherein the variant has one or more mutations selected from the group consisting of R28, E34, D37, D174, Y223, C339, Y371, and M173, whereby a sugar phosphate is produced; and
(b) incubating the sugar phosphate with a nucleotidylyltransferase, whereby a NDP-sugar is produced.
11. The method of claim 10 , wherein the D or L sugar is selected from the group consisting of D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose.
12. The method of claim 10 , wherein the nucleotidylyltransferase is Ep or a mutated variant thereof.
13. The method of claim 12 , wherein the mutated Ep variant is mutated at one or more amino acids selected from the group consisting of V173, G147, W224, N112, G175, D111, E162, T201, I200, E199, R195, L89, L89T, L109, Y146 and Y177.
14. The method of claim 10 , wherein the NTP is ATP.
15. The method of claim 10 , wherein the GalK variant is Y371H, M173L or Y371H-M173L.
16. The method of claim 10 , wherein the method is carried out in vitro.
17. The method of claim 10 , wherein the method is carried out in a host cell.
18. The method of claim 17 , wherein the host cell is a bacterium.
19. The method of claim 17 , wherein the host cell is selected from the group consisting of E. coli and S. lividans.
20. A method of producing a glycosylated biomolecule containing at least one sugar moeity, comprising the steps of:
(a) incubating a nucleotide triphosphate (NTP) and a D or L sugar in the presence of a GalK variant of the wild type E. coli GalK amino acid sequence set forth in SEQ ID NO: 1, wherein the variant has one or more mutations selected from the group consisting of R28, E34, D37, D174, Y223, C339, Y371, and M173, whereby a sugar phosphate is produced;
(b) incubating the sugar phosphate with a nucleotidylyltransferase, whereby a NDP-sugar is produced; and
(c) incubating the NDP-sugar with a biomolecule capable of being glycosylated in the presence of a glycosyltransferase, whereby a glycosylated biomolecule is produced.
21. The method of claim 20 , wherein the D or L sugar is selected from the group consisting of D-galactose, 2-deoxy D-galactose, D-galactose-amine, D-talose, 3-deoxy-D-galactose, 6-deoxy-D-galactose, 6-amino-D-galactose, D-galacturonic acid, L-altrose and L-glucose.
22. The method of claim 20 , wherein the nucleotidylyltransferase is Ep or a mutated variant thereof.
23. The method of claim 22 , wherein the mutated Ep variant is mutated at one or more amino acids selected from the group consisting of V173, G147, W224, N112, G175, D111, E162, T201, I200, E199, R195, L89, L89T, L109, Y146 and Y177.
24. The method of claim 20 , wherein the glycosyltransferase is selected from the group consisting of CalB, CalE, CalN, CalU, Gra orf14, Gra orf5, LanGT1, LanGT2, LanGT3, LanGT4, MtmGI, MtmGII, MtmGTIII, MtmGTIV, NovM, RhlB, Rif orf 7, SnogD, SnogE, SnogZ, UrdGT1a, UrdGT1b, UrdGT1c, UrdGT2, AknK, AknS, DesVII, DnrS, OleG1, OleG2, TylCV, TylMII, TylN, DauH, DnrH, EryBV, EryCIII, Ngt, BgtA, BgtB, BgtC, GftA, GftB, GftC, GftD, GftE, Gp1-1, Gp1-2, RtfA, AveBI, BlmE, BlmF, MgtA, NysD1, OleD, OleI, SpcF, SpcG, StrH, Ugt51B1, Ugt51C1, UGT52, UgtA, UgtB, UgtC, UgtD and homologs thereof.
25. The method of claim 20 , wherein the NTP is ATP.
26. The method of claim 20 , wherein the GalK variant is Y371H, M173L or Y371H-M173L.
27. The method of claim 20 , wherein the method is carried out in vitro.
28. The method of claim 20 , wherein the method is carried out in a host cell.
29. The method of claim 28 , wherein the host cell is a bacterium.
30. The method of claim 28 , wherein the host cell is selected from the group consisting of E. coli and S. lividans.
31. The method of claim 20 , wherein the biomolecule capable of being glycosylated is selected from the group consisting of natural and synthetic metabolites, pyran rings, furan rings, enediynes, anthracyclines, angucyclines, aureolic acids, orthosomycins, macrolides, aminoglycosides, non-ribosomal peptides, polyenes, steroids, lipids, indolocarbazoles, bleomycins, amicetins, benzoisochromanequinones coumarins, polyketides, pluramycins, aminoglycosides, oligosaccharides, peptides, proteins, hybrids consisting of one or more these components, analogs and bioactive aglycons thereof.
32. A method of claim 20 , wherein the glycosylated biomolecule is further incubated with at least one chemoselectively ligatable moiety, whereby at least one chemoselectively ligated compound is produced.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US12/356,208 US20090137006A1 (en) | 2003-12-05 | 2009-01-20 | Sugar Kinases with Expanded Substrate Specificity and Their Use |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US48174203P | 2003-12-05 | 2003-12-05 | |
US10/904,941 US7479385B2 (en) | 2003-12-05 | 2004-12-06 | Sugar kinases with expanded substrate specificity and their use |
US12/356,208 US20090137006A1 (en) | 2003-12-05 | 2009-01-20 | Sugar Kinases with Expanded Substrate Specificity and Their Use |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US10/904,941 Division US7479385B2 (en) | 2003-12-05 | 2004-12-06 | Sugar kinases with expanded substrate specificity and their use |
Publications (1)
Publication Number | Publication Date |
---|---|
US20090137006A1 true US20090137006A1 (en) | 2009-05-28 |
Family
ID=34676549
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US10/904,941 Expired - Fee Related US7479385B2 (en) | 2003-12-05 | 2004-12-06 | Sugar kinases with expanded substrate specificity and their use |
US12/356,208 Abandoned US20090137006A1 (en) | 2003-12-05 | 2009-01-20 | Sugar Kinases with Expanded Substrate Specificity and Their Use |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US10/904,941 Expired - Fee Related US7479385B2 (en) | 2003-12-05 | 2004-12-06 | Sugar kinases with expanded substrate specificity and their use |
Country Status (2)
Country | Link |
---|---|
US (2) | US7479385B2 (en) |
WO (1) | WO2005056786A2 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2012024678A2 (en) * | 2010-08-20 | 2012-02-23 | Zuchem, Inc. | Activated sugars |
US11851036B2 (en) | 2018-04-23 | 2023-12-26 | Oshkosh Corporation | Integrated ground pad |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8097443B2 (en) | 2007-04-16 | 2012-01-17 | Wisconsin Alumni Research Foundation | Nucleotidyltransferases with enhanced nucleotide triphosphate flexibility |
US8093028B2 (en) | 2007-07-24 | 2012-01-10 | Wisconsin Alumni Research Foundation | Engineered glycosyltransferases with expanded substrate specificity |
US8278073B2 (en) | 2007-12-20 | 2012-10-02 | Universiteit Gent | Lactose phosphorylase enzymes |
JP2013542724A (en) * | 2010-10-13 | 2013-11-28 | ディーエスエム アイピー アセッツ ビー.ブイ. | Polypeptide having permease activity |
EP2652130B1 (en) * | 2010-12-14 | 2021-11-17 | Universiteit Gent | Trehalose phosphorylases and their use in the biocatalytic production of trehalose-analogues and glycosyl phosphates |
AU2015363113B2 (en) * | 2014-12-17 | 2021-03-11 | E. I. Du Pont De Nemours And Company | Compositions and methods for efficient gene editing in E. coli using guide RNA/Cas endonuclease systems in combination with circular polynucleotide modification templates |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2002226085A1 (en) | 2000-12-13 | 2002-06-24 | Memorial Sloan-Kettering Cancer Center | Active-site engineering of nucleotidyltransferases and enzymatic methods for the synthesis of natural and "unnatural" udp- and nucleotide sugars |
WO2002079150A2 (en) | 2001-03-30 | 2002-10-10 | Sloan-Kettering Institute For Cancer Research | Glycorandomization and the production of novel erythronolide and coumarin analogs |
-
2004
- 2004-12-06 WO PCT/US2004/040942 patent/WO2005056786A2/en active Application Filing
- 2004-12-06 US US10/904,941 patent/US7479385B2/en not_active Expired - Fee Related
-
2009
- 2009-01-20 US US12/356,208 patent/US20090137006A1/en not_active Abandoned
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2012024678A2 (en) * | 2010-08-20 | 2012-02-23 | Zuchem, Inc. | Activated sugars |
WO2012024678A3 (en) * | 2010-08-20 | 2013-05-10 | Zuchem, Inc. | Activated sugars |
US11851036B2 (en) | 2018-04-23 | 2023-12-26 | Oshkosh Corporation | Integrated ground pad |
Also Published As
Publication number | Publication date |
---|---|
WO2005056786A2 (en) | 2005-06-23 |
WO2005056786A3 (en) | 2005-09-01 |
US20050208633A1 (en) | 2005-09-22 |
US7479385B2 (en) | 2009-01-20 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US20090137006A1 (en) | Sugar Kinases with Expanded Substrate Specificity and Their Use | |
Yang et al. | Structure-based engineering of E. coli galactokinase as a first step toward in vivo glycorandomization | |
Griffith et al. | ‘Sweetening’natural products via glycorandomization | |
Hoffmeister et al. | Creation of the first anomeric D/L-sugar kinase by means of directed evolution | |
Kubo et al. | Alteration of sugar donor specificities of plant glycosyltransferases by a single point mutation | |
Persson et al. | A high-throughput pH indicator assay for screening glycosyltransferase saturation mutagenesis libraries | |
US9102922B2 (en) | Trehalose phosphorylases and their use in the biocatalytic production of trehalose-analogues and glycosyl phosphates | |
Feng et al. | Regio‐and stereospecific O‐glycosylation of phenolic compounds catalyzed by a fungal glycosyltransferase from Mucor hiemalis | |
EP1842910A1 (en) | Method of enzymatically synthesizing 3'-phosphoadenosine-5'-phosphosulfate | |
US10494616B2 (en) | Cellobiose phosphorylase | |
Borg et al. | Mechanistic characterization of UDP‐glucuronic acid 4‐epimerase | |
CN110699373A (en) | Uridine diphosphate glucose high-producing strain and application thereof | |
Pei et al. | Construction of a novel UDP-rhamnose regeneration system by a two-enzyme reaction system and application in glycosylation of flavonoid | |
Zhang et al. | Efficient chemoenzymatic synthesis of globotriose and its derivatives with a recombinant α-(1→ 4)-galactosyltransferase | |
US20160272953A1 (en) | Activated Sugars | |
Li et al. | An artificial multi-enzyme cascade biocatalysis for biomanufacturing of nicotinamide mononucleotide from starch and nicotinamide in one-pot | |
Dion et al. | Modulation of the regioselectivity of a Bacillus α-galactosidase by directed evolution | |
Elling et al. | An enzyme module system for the synthesis of dTDP‐activated deoxysugars from dTMP and sucrose | |
Zou et al. | One-pot three-enzyme synthesis of UDP-Glc, UDP-Gal, and their derivatives | |
Kharel et al. | Characterization of the TDP-D-ravidosamine biosynthetic pathway: one-pot enzymatic synthesis of TDP-D-ravidosamine from thymidine-5-phosphate and glucose-1-phosphate | |
Yi et al. | Insights into the Functionalization of the Methylsalicyclic Moiety during the Biosynthesis of Chlorothricin by Comparative Kinetic Assays of the Activities of Two KAS III‐like Acyltransferases | |
Dai et al. | Dictyoglomus turgidum DSM 6724 α-Glucan phosphorylase: Characterization and its application in multi-enzyme cascade reaction for d-tagatose production | |
US20180320149A1 (en) | High yields of isomelezitose from sucrose by engineered glucansucrases | |
Shi et al. | Substrate promiscuities of a bacterial galactokinase and a glucose-1-phosphate uridyltransferase enable xylose salvaging | |
JP2023537880A (en) | Biosynthetic production of 2-fucosyllactose |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
STCB | Information on status: application discontinuation |
Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION |