US20080145315A1 - Protein fragment complementation assays in whole animals applications to drug efficacy, adme, cancer biology, immunology, infectious disease and gene therapy - Google Patents
Protein fragment complementation assays in whole animals applications to drug efficacy, adme, cancer biology, immunology, infectious disease and gene therapy Download PDFInfo
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- US20080145315A1 US20080145315A1 US11/987,694 US98769407A US2008145315A1 US 20080145315 A1 US20080145315 A1 US 20080145315A1 US 98769407 A US98769407 A US 98769407A US 2008145315 A1 US2008145315 A1 US 2008145315A1
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/03—Animal model, e.g. for test or diseases
- A01K2267/0331—Animal model for proliferative diseases
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/03—Animal model, e.g. for test or diseases
- A01K2267/0393—Animal model comprising a reporter system for screening tests
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A90/00—Technologies having an indirect contribution to adaptation to climate change
- Y02A90/10—Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation
Definitions
- the present invention relates to the determination of the function of novel gene products.
- the invention further relates to Protein fragment Complementation Assays (PCA).
- PCAs allow for the detection of a wide variety of types of protein-protein, protein-RNA, protein-DNA, Protein-carbohydrate or protein-small organic molecule interactions in different cellular contexts appropriate to the study of such interactions.
- Approaches include the use of chemical crosslinking reagents and resonance energy transfer between dye-coupled proteins 102, 103 .
- a powerful and commonly used strategy the yeast two-hybrid system, is used to identify novel protein-protein interactions and to examine the amino acid determinants of specific protein interactions 4,6-8 .
- the approach allows for rapid screening of a large number of clones, including cDNA libraries. Limitations of this technique include the fact that the interaction must occur in a specific context (the nucleus of S. cerevisiae ), and generally cannot be used to distinguish induced versus constitutive interactions.
- USPS ubiquitin-based split protein sensor
- the strategy is based on cleavage of proteins with N-terminal fusions to ubiquitin by cytosolic proteases (ubiquitinases) that recognize its tertiary structure.
- the strategy depends on the reassembly of the tertiary structure of the protein ubiquitin from complementary N- and C-terminal fragments and crucially, on the augmention of this reassembly by oligomerization domains fused to these fragments.
- this application describes a general strategy to develop molecular interaction assays from a large number of enzyme or protein detectors, all de novo designed assays, whereas the ⁇ -gal assay is not novel, nor are any general strategies or advancements over previously well documented applications given.
- the yeast-two hybrid strategy requires additional cellular machinery for detection that exist only in specific cellular compartments.
- the assay would involve an oligomerization-assisted complementation of fragments of monomeric or multimeric enzymes that require no other proteins for the detection of their activity.
- the structure of an enzyme were known it would be possible to design fragments of the enzyme to ensure that the reassembled fragments would be active and to introduce mutations to alter the stringency of detection of reassembly.
- knowledge of structure is not a prerequesite to the design of complementing fragments, as will be explained below. The flexibility allowed in the design of such an approach would make it applicable to situations where other detection systems may not be suitable.
- a first step in defining the function of a novel gene is to determine its interactions with other gene products in an appropriate context; that is, since proteins make specific interactions with other proteins or other biopolymers as part of functional assemblies, an appropriate way to examine the function of a novel gene is to determine its physical relationships with the products of other genes.
- the present invention seeks to provide the above-mentioned needs for which the prior art is silent.
- the present invention provides a general strategy for detecting protein interactions with other biopolymers including other proteins, nucleic acids, carbohydrates or for screening small molecule libraries for compounds of potential therapeutic value.
- the instant invention seeks to provide an oligomerization-assisted complementation of fragments of monomeric enzymes that require no other proteins for the detection of their activity.
- a protein-fragment complementation assay based on reconstitution of dihydrofolate reductase activity by complementation of defined fragments of the enzyme in E. coli is hereby provided. This assay requires no additional endogenous factors for detecting specific protein-protein interactions (i.e.
- the assay can also be adapted for detection of protein interactions in any cellular context or compartment and be used to distinguish between induced versus constitutive protein interactions in both prokaryotic and eukaryotic systems.
- PCA protein complementation assay
- the structure of the enzyme is used to decide the best position in the polypeptide chain to split the gene in two, based on the following criteria: 1) The fragments should result in subdomains of continuous polypeptide; that is, the resulting fragments will not disrupt the subdomain structure of the protein, 2) the catalytic and cofactor binding sites should all be contained in one fragment, and 3) resulting new N- and C-termini should be on the same face of the protein to avoid the need for long peptide linkers and allow for studies of orientation-dependence of protein binding.
- the enzyme be small, preferably between 10-40 kDa. Although monomeric enzymes are preferred, multimeric enzymes can also be envisaged as within the scope of the present invention.
- the dimeric protein tyrosinase can be used in the instant assay.
- the information on the structure of the enzyme provides an additional advantage in designing the PCA, but is not necessary. Indeed, an additional strategy, to develop PCAs is presented, based on a combination of exonuclease digestion-generated protein fragments followed by directed protein evolution in application to the enzyme aminoglycoside kinase. Although the overexpression in prokaryotic cells is preferred it is not a necessity. It will be understood to the skilled artisan that the enzyme catalytic site (of the chosen enzyme) does not absolutely need to be on same molecule.
- FIG. 1 shows a general description of a PCA.
- the gene for a protein or enzyme is rationally dissected into two or more fragments. Using molecular biology techniques, the chosen fragments are subcloned, and to the 5′ ends of each, proteins that either are known or thought to interact are fused. Co-transfection or transformation these DNA constructs into cells is then carried out. Reassembly of the probe protein or enzyme from its fragments is catalyzed by the binding of the test proteins to each other, and reconstitution is observed with some assay. It is crucial to understand that these assays will only work if the fused, interacting proteins catalyze the reassembly of the enzyme. That is, observation of reconstituted enzyme activity must be a measure of the interaction of the fused proteins.
- a preferred embodiment of the present invention focuses on a PCA based on the enzyme dihydrofolate reductase. Expansion of the strategy to include assays in eukaryotic, cells, library screening, and a specific application to problems concerning the study of integrated biochemical pathways such as signal transduction pathways, is presented. Additional assays, including those based on enzymes that can act as dominant or recessive drug selection or metabolic salvage pathways are disclosed. In addition, PCAs based on enzymes that will produce a colored or fluorescent product are also disclosed. The present invention teaches how the PCA strategy can be both generalized and automated for functional testing of novel genes, screening of natural products or compound libraries for pharmacological activity and identification of novel gene products that interact with DNA, RNA or carbohydrates are disclosed.
- PCA strategy can be applied to identifying natural products or small molecules from compound libraries of potential therapeutic value that can inhibit or activate such molecular interactions and how enzyme substrates and small molecule inhibitors of enzymes can be identified. Finally, it teaches how the PCA strategy can be used to perform protein engineering experiments that could lead to designed enzymes with industrial applications or peptides with biological activity.
- a protein-fragment complementation strategy can be used to study equilibrium and kinetic aspects of protein-protein interactions in vivo.
- the DHFR and other PCAs are simpler assays. They are complete systems; no additional endogenous factors are necessary and the results of complementation are observed directly, with no further manipulation.
- the E. coli cell survival assay described herein should therefore be particularly useful for screening cDNA libraries for protein-protein interactions.
- mDHFR expression in cells can be monitored by binding of fluorescent high-affinity substrate analogues for DHFR 26 .
- the present invention should not be limited to the DHFR or other PCAs presented, as it is only non-limiting embodiments of the protein complementation assay of the present invention.
- the PCAs should not be limited in the context in which they could be used.
- Constructs could be designed for targeting the PCA fusions to specific compartments in the cell by addition of signaling peptide sequences 27,28 .
- Induced versus constitutive protein-protein interactions could be distinguished by a eukaryotic version of the PCA, in the case of an interaction that is triggered by a biochemical event.
- the system could be adapted for use in screening for novel, induced protein-molecular associations between a target protein and an expression library.
- the instant invention is also directed to a method for detecting biomolecular interactions said method comprising:
- the invention also provides molecular fragment complementation assays for the detection of molecular interactions comprising a reassembly of separate fragments of a molecule, wherein reassembly of said fragments is operated by the interaction of molecular domains fused to each fragment of said molecules, and wherein reassembly of the fragments is independent of other molecular processes.
- the present invention is directed to a method of testing biomolecular interactions comprising:
- the invention is directed to a method comprising an assay where fragments of a first molecule are fused to a second molecule and fragment association is detected by reconstitution of the first molecule's activity.
- the present invention also provides a composition comprising a product selected from the group consisting of:
- the invention further provides a composition comprising complementary fragments of a first molecule, each fused to a separate fragment of a second molecule.
- compositions comprising a nucleic acid molecule coding for a fusion product, which molecule comprises sequences coding for a product selected from the group consisting of:
- the present invention is also directed to a method of testing for biomolecular interactions associated with: (a) complementary fragments of a first molecule whose fragments can exhibit a detectable activity when associated or (b) binding of two protein-protein interacting domains from a second or third molecule, said method comprising:
- the instant invention further provides a composition comprising a product selected from the group consisting of:
- the invention is also directed to a composition
- a composition comprising a nucleic acid molecule coding for a fusion product, which molecule comprises sequences coding for either:
- the invention also provides a method of detecting kinetics of protein assembly and screening cDNA libraries comprising performing PCA.
- the invention further provides a method of testing the ability of a compound to inhibit molecular interactions in a PCA comprising performing a PCA in the presence of said compound and correlating any inhibition with said presence.
- the invention provides a method for detecting protein-protein interactions in living organisms and or cells, which method comprises:
- the invention still provides a method for detecting biomolecular interactions said method comprising:
- the invention also provides a novel method of affecting gene therapy, which includes the step of providing the assays and compositions described above.
- the present invention is pioneering as it is the first protein complementation assay displaying such a level of simplicity and versatility.
- the exemplified embodiments are protein-fragment complementation assays (PCA) based on mDHFR, where a leucine zipper directs the reconstitution of DHFR activity. Activity was detected by an E. coli survival assay which is both practical and inexpensive.
- PCA protein-fragment complementation assays
- E. coli survival assay which is both practical and inexpensive.
- This system illustrates the use of mDHFR fragment complementation in the detection of leucine zipper dimerization and could be applied to the detection of unknown, specific protein-molecular interactions in vivo.
- any useful reporter can be engineered for PCA, any readout can be generated, including cell survival under selective pressure; calorimetric; fluorescent; luminescent; or phosphorescent signals.
- PCA in whole animals can be performed either by starting with nucleic acids encoding the compositions of interest; or by starting with stable cell lines that have been created by transfection or co-transfection of the nucleic acid compositions of interest.
- nucleic acid compositions encoding genes of interest that have been tagged with PCA reporter fragments can be introduced into whole organisms either by injection or by the creation of transgenic animals. With transgenic animals, the gene/reporter construct(s) will be present in all cells in the body. With injection, specific organs can be targeted or the constructs can be injected into the bloodstream. For example, an engineered HIV virus can be created with reporter constructs in frame with sequences coding for HIV proteins, and injected directly into the bloodstream in order to study the process of viral infection and the effects of antiviral drugs.
- stable cell lines are created by standard methods involving transfection or co-transfection of the nucleic acid compositions of interest, followed by selective pressure to allow the growth of cells expressing or co-expressing the proteins of interest.
- any cell type and reporter can be used.
- the cells are then introduced into animals by one of several methods.
- transformed cells are used in the nude mouse model to create mice with tumors comprised of the cells that express or co-express the fusion proteins of interest.
- cells are grown intraperitoneally, in a manner similar to that used for the creation of monoclonal antibodies from hybridomas. These mice can be used to study ADME (absorption, distribution, metabolism and excretion) of drugs targeted against specific proteins.
- ADME absorption, distribution, metabolism and excretion
- cells are injected into the bloodstream or into specific organs of interest in order to study biological processes such as extravasation, migration, metastasis, and tumor growth; and the effects of anticancer drugs.
- engineered bacteria can be injected into animals to study the infection process and the effects of antimicrobial agents.
- PCA will also provide for the study of immune responses, allergy, transplant rejection, and the effects of drugs or drug candidates on those processes.
- transgenic animals can be created that express a single fusion product or two complementary fusion products.
- every cell in the body would be expected to harbor the genes of interest, and individual cells or organs may express the complementary fusion products depending on the promoter under which the gene constructs are regulated.
- the complementary fusion product can be introduced into the transgenic animal by one of the methods above for introduction of nucleic acids. For example, this can be used to study viral/host protein-protein interactions by creating a transgenic animal in which the host protein of interest is tagged with the first fragment of the reporter, and the viral protein of interest is tagged with the second fragment of the reporter. Infection leads to expression of the tagged viral protein which, upon interaction with the complementary tagged host protein, generates a signal.
- Detection of PCA in vivo can be done by whole body imaging methods or by endoscopy.
- a variety of reporters can be used including those originally specified in U.S. Pat. No. 6,270,964 which provide for the generation of survival-selection, fluorescent, luminescent or phosphorescent signals.
- survival-selection PCA cells can be created that will survive (in nude mice, or as ascites) only when two proteins interact. This can be done by creating PCA with an antibiotic resistance reporter gene such as hygromycin phosphotransferase, and feeding the animals antibiotics. If the protein-protein interaction of interest occurs the transplanted cells will survive, otherwise they will die.
- using a “cell-death PCA” cells can be created that will survive only when a drug disrupts a protein-protein interaction.
- biophotonic imaging systems can be used to detect the signal.
- Xenogen Corporation see www.xenogen.com and references therein
- biophotonic systems see www.biophotonics-mag.com.
- biophotonic approaches have not previously been used with PCA; the molecular techniques previously used to generate a signal have only allowed the detection of unimolecular events such as the expression of a single tagged gene, or have used alternative technologies such as fluorescence resonance energy transfer (FRET).
- FRET fluorescence resonance energy transfer
- PCA can be used to eliminate the need for traditional ADME approaches, by using whole-body imaging to detect the effect of the pharmaceutical agent on the target within living tissues.
- fluorescence PCA has been used to detect activation of the erythropoietin receptor in living cells in response to erythropoietin or a peptide mimetic, EMP1 (Remy and Michnick).
- Cells that stably express the EPO receptor/PCA fragment constructs can be introduced into animals as described under methods.
- Suitable PCA reporters include but are not limited to luciferase, GFP, and beta-lactamase. The small molecule EPO mimetics would then be administered to the mice.
- ADME Alzheimer's disease
- the reporter will reassemble from its complimentary fragments, leading to a detectable signal. Fluorescence or luminescence can then detected by whole body imaging. The signal will persist as long as the pharmaceutical agent is active. If the mimetic is rapidly degraded or excreted, the signal will diminish.
- This is a “live” approach to ADME that does not require serial sampling of body fluids, and eliminates the need for analysis of the compound itself within the body fluids thereby saving time and money.
- ADME only requires serial imaging of the animal over a period of time to establish the time course of activity of the agent. Similar approaches can be applied to other biopharmaceuticals such as cytokines, hormones, and mimetics of these agents.
- in vivo PCA can be applied to studies of efficacy and pharmacokinetics of receptor antagonists e.g. Herceptin, and like compounds.
- receptor antagonists e.g. Herceptin, and like compounds.
- these approaches can be applied to any agent that blocks a signaling pathway or a biomolecular event within cells, by simply tagging the appropriate genes with the reporter fragments, creating cell lines, and establishing the cells in animals.
- in vivo PCA can be applied by creating transgenic mice that express the fusion constructs in every cell line, thereby enabling studies of the distribution of the pharmaceutical agent within tissues.
- PCA in vivo PCA can be used to study cancer biology, to track cancer cells, and to develop new anticancer drugs against specific targets.
- Tumor cells stably expressing genes of interest are established in mice.
- cells stably expressing the Her-2/neu oncoprotein (receptor) together with reporter construct can be used to study the effects of Herceptin.
- a suitable PCA reporter such as hygromycin phosphotransferase, can be fused to Her-2/neu genes to create cell lines that only survive under antibiotic treatment if the receptor is active (dimerized in active form). Herceptin would be expected to disrupt the association of the receptor leading to a reduction in tumor formation or size.
- PCAs with light-emitting PCAs, cells stably expressing the receptor constructs that serve as a biosensor for the presence of agonist/antagonist can be injected into animals. If tumors are formed they can be detected as light-emitting clusters of cells. The size and location of the tumor, and the intensity of the light, can be determined by imaging. Drugs which antagonize the receptor would diminish the signal.
- This application of PCA biosensors is not limited to receptors but can be applied to any biomolecular interaction that is of interest for cancer biology and anticancer drug development.
- the drug FK506 is a powerful immunophilin which has specific biochemical effects including competitive inhibition of the rapamycin-induced interaction of the FK506 binding protein (FKBP) and FRAP/TOR/RAFT.
- FKBP FK506 binding protein
- FRAP/TOR/RAFT FK506 binding protein
- PCA can be used to study the pharmacokinetics of the association of these proteins and its inhibition by FK506.
- Immortalized cells stably expressing FKBP/TOR with PCA constructs can be introduced into mice (or transgenic mice can be created with the fusion constructgs).
- Administration of rapamycin to the animal would induce the association of FKBP/TOR leading to a light-emitting signal from the cells.
- FK506 would prevent the association of FKBP/TOR and would reduce the signal.
- Dose-responses of FK506 and similar agents could be established in this way.
- PCA In vivo PCA could also be used to study infectious diseases and gene therapy.
- FIG. 1 provides a general description of a PCA. Using molecular biology techniques, the chosen fragments of the enzyme are subcloned, and to the 5′ ends of each, proteins that either are known or thought to interact are fused. Co-transfection or transformation these DNA constructs into cells is then carried out and reconstitution with some assay is observed.
- FIG. 2 is a scheme of the fusion constructs used in one of the embodiments of the invention.
- the hexahistidine peptide (6His), the homodimerizing GCN4 leucine zipper (Zipper) and mDHFR fragments (1, 2 and 3) are illustrated.
- the labels for the constructs are used to identify both the DNA constructs and the proteins expressed from these constructs.
- FIG. 3 (A) shows E. coli survival assay on minimal medium plates.
- Control Left side of the plate: E. coli harboring pQE-30 (no insert); right side: E. coli harboring pQE-16, coding for native mDHFR.
- Panel I Left side of each plate: transformation with construct Z-F[1,2]; right side of each plate: transformation with construct Z-F[3].
- Panel II Cotransformation with constructs Z-F[1,2] and Z-F[3].
- Panel III Cotransformation with constructs Control-F[1,2] and Z-F[3]. All plates contain 0.5 mg/ml trimethoprim. In panels I to III, plates on the right side contain 1 mM IPTG.
- FIG. 1 E. coli survival assay using destabilizing DHFR mutants.
- Panel I Cotransformation of E. coli with constructs Z-F[1,2] and Z-F[3:Ile114Val].
- Panel II Cotransformation with Z-F[1,2] and Z-F[3:Ile114Ala]. Inset is a 5-fold enlargement of the right-side plate.
- Panel III Cotransformation with Z-F[1,2] and Z-F[3:Ile114Gly]. All plates contain 0.5 mg/ml trimethoprim. Plates on the right side contain 1 mM IPTG.
- FIG. 4 features the coexpression of mDHFR fragments.
- A Agarose gel analysis of restriction pattern resulting from HincII digestion of plasmid DNA.
- Lane 1 contains DNA isolated from E. coli cotransformed with constructs Z-F[1,2] and Z-F[3].
- Lanes 2 and 3 contain DNA isolated from E. coli transformed with, respectively, construct Z-F[3] and construct Z-F[1,2]. Fragment migration (in bp) is indicated to the right.
- Lanes 1 to 5 show crude lysate of untransformed E. coli (lane 1), or E. coli expressing Z-F[1,2] (20.8 kDa; lane 2), Z-F[3] (18.4 kDa; lane 3), Control-F[1,2] (14.2 kDa; lane 4), and Z-F[1,2]+Z-F[3] (lane 5).
- Lane 6 shows 40 ml out of 2 ml copurified Z-F[1,2] and Z-F[3]. Arrowheads point to the proteins of interest. Migration of molecular weight markers (in kDa) is indicated to the right.
- FIG. 5 illustrates the general features of a PCA based on a survival assay such as the DHFR PCA.
- the assay can be used in a bacterial or a mammalian context.
- the inserted target DNA can be a known sequence coding for a protein (or protein domain) of interest, or can be a cDNA library.
- FIG. 6 represents an autoradiograph of a COS cell lysate after a 30 min. 35 S-Met-Cys pulse-labelling.
- the expression pattern is essentially identical to that observed in E. coli (see FIG. 4 ).
- the DNA transfected into the cells (or cotransfected) is indicated above the respective lanes.
- FIG. 7 illustrates the results of a protein engineering application of the mDHFR bacterial PCA.
- Two semi-random leucine zipper libraries were created (as described in the text) and each inserted N-terminal to one of the mDHFR fragments.
- Cotransformation of the resulting zipper-DHFR fragment libraries in E. coli and plating on selective medium allowed for survival of clones harboring successfully interacting leucine zippers.
- Fourteen clones were isolated and the zippers were sequenced to identify the residues at the “e” and “g” positions.
- the “e-g” pairs were categorized, as having attractive pairing (charge:charge, charge:neutral polar or neutral polar:neutral polar) or repulsive pairing (charge:charge) and the number of each type of interaction scored for each clone.
- the total number of interactions for each clone is 6; the interactions are tallied on the histogram.
- FIG. 8 illustrates the construction of expression vectors for PCA with GFP fragments.
- FIG. 9 shows the demonstration of a protein-fragment complementation assay based on green fluorescent protein (GFP). Homodimerization of GCN4 leucine zipper-forming sequences is shown in mammalian (COS-7) and human (HEK293T) cells.
- GFP green fluorescent protein
- FIG. 10 illustrates PCA in human cell lines, showing the fluorescent signal generated by the interactions of two different pairs of full-length proteins.
- a mutant (yellow) version of GFP was used to generate the protein-fragment complementation assay.
- FIG. 11 describes the PCA strategy for the detection of molecular interactions in whole organisms. PCA in situ in a mouse xenograft model is demonstrated.
- PCA protein-fragment complementation assay
- DHFRs are small (17 kD to 21 kD), monomeric proteins.
- the crystal structures of DHFR from various bacterial and eukaryotic sources are known and substrate binding sites and active site residues have been determined 111-114 , allowing for rational design of protein fragments.
- the folding, catalysis, and kinetics of a number of DHFRs have been studied extensively 115-119 .
- the enzyme activity can be monitored in vitro by a simple spectrophotometric assay 120 , or in vivo by cell survival in cells grown in the absence of DHFR end products.
- DHFR is specifically inhibited by the anti-folate drug trimethoprim.
- mammalian DHFR has a 12000-fold lower affinity for trimethoprim than does bacterial DHFR 121 , growth of bacteria expressing mDHFR in the presence of trimethoprim levels lethal to bacteria is an efficient means of selecting for reassembly of mDHFR fragments into active enzyme.
- High level expression of mDHFR has been demonstrated in transformed prokaryote or transfected eukaryotic cel s 122-126 .
- mDHFR shares high sequence identity with the human DHFR (hDHFR) sequence (91% identity) and is highly homologous to the E. coli enzyme (29% identity, 68% homology) and these sequences share visually superimposable tertiary structure 111 . Comparison of the crystal structures of mDHFR and hDHFR suggests that their active sites are essentially identical 127,128 .
- the folate binding pocket and the NADPH binding groove are formed mainly by residues belonging to fragments[1] and [2]. Fragment [3] is not directly implicated in catalysis.
- the native N-terminus of mDHFR and the novel N-terminus created by cleavage occur on the same surface of the enzyme 112, 128 allowing for ease of N-terminal covalent attachment of each fragment to associating fragments such as the leucine zippers used in this study.
- leucine-zipper assisted assembly of the mDHFR fragments into active enzyme we have obtained leucine-zipper assisted assembly of the mDHFR fragments into active enzyme.
- Mutagenic and sequencing oligonucleotides were purchased from Gibco BRL. Restriction endonucleases and DNA modifying enzymes were from Pharmacia and New England Biolabs. The mDHFR fragments carrying their own in-frame stop codon were subcloned into pQE-32 (Qiagen), downstream from and in-frame with the hexahistidine peptide and a GCN4 leucine zipper ( FIG. 1 ; FIG. 2 ).
- Residues 235 to 281 of the GCN4 leucine zipper were obtained from a yeast expression plasmid pRS316 9 .
- the recessed terminus at the BamHI site was filled-in with Klenow polymerase and the fragment was ligated to pQE-32 linearized with SalI/HindIII(filled-in).
- the product, construct Z carries an open reading frame coding for the sequence Met-Arg-Gly-Ser followed by a hexahistidine tag and 13 residues preceding the GCN4 leucine zipper residues.
- the eukaryotic transient expression vector, pMT3 (derived from pMT2) 16 , was used as a template for PCR-generation of mDHFR containing the features allowing subcloning and separate expression of fragment[1,2] and fragment[3].
- the megaprimer method of PCR mutagenesis 29 was used to generate a full-length 590 bp product. Oligonucleotides complementary to the nucleotide sequence coding for the N- and C-termini of mDHFR and containing a novel BspEI site outside the coding sequence were used as well as an oligonucleotide used to create a novel stop codon after fragment[1,2], followed by a novel SpeI site for use in subcloning fragment[3].
- the 590 bp PCR product (described above) was digested with BspEI and inserted into pMT3 linearized at BspEI, yielding construct [1,2,3].
- the 610 bp BspEI/EcoNI fragment (coding for DHFR fragment[1,2], followed by a novel stop and fragment[3] up to EcoNI) was filled in at EcoNI and subcloned into pMT3 opened with BspEI/HpaI, yielding construct F[1,2].
- the 250 bp SpeI/BspEI fragment of construct [1,2,3] coding for DHFR fragment[3] (with no in-frame stop codon) was subcloned into pMT3 opened with the same enzymes.
- Site-directed mutagenesis was performed 30 to produce mutants at Ile14 (numbering of the wild-type mDHFR).
- the mutagenesis reaction was carried out on the KpnI/BamHI fragment of construct Z-F[3] subcloned into pBluescript SK+ (Stratagene), using oligonucleotides that encode a silent mutation producing a novel BamHI site.
- the 206 bp NheI/EcoNI fragment of putative mutants identified by restriction was subcloned back into Z-F[3]. The mutations were confirmed by DNA sequencing.
- E. coli strain BL21 carrying plasmid pRep4 (from Qiagen, for constitutive expression of the lac repressor) were made competent, transformed with the appropriate DNA constructs and washed twice with minimal medium before plating on minimal medium plates containing 50 mg/ml kanamycin, 100 mg/ml ampicillin and 0.5 mg/ml trimethoprim. One half of each transformation mixture was plated in the absence, and the second half in the presence, of 1 mM IPTG. All plates were placed at 37° C. for 66 hrs.
- Colonies obtained from cotransformation were propagated and used to inoculate 10 ml of minimal medium supplemented with ampicillin, kanamycin as well as IPTG (1 mM) and trimethoprim (1 ⁇ g/ ⁇ l) where indicated.
- Cotransformants of Z-F[1,2]+Z-F[3:Ile114Gly] were obtained under non-selective conditions by plating the transformation mixture on L-agar (+kanamycin and ampicillin) and screening for the presence of the two constructs by restriction analysis. All growth curves were performed in triplicate. Aliquots were withdrawn periodically for measurement of optical density. Doubling time was calculated for early logarithmic growth (OD 600 between 0.02 and 0.2).
- Bacteria were propagated in Terrific Broth 31 in the presence of the appropriate antibiotics to an OD600 of approximately 1.0. Expression was induced by addition of 1 mM IPTG and further incubation for 3 hrs.
- pellets from 150 ml of induced cells were lysed by boiling in loading dye. The lysates were clarified by microcentrifugation and analyzed by SDS-PAGE32.
- protein purification a cell pellet from 50 ml of induced E. coli cotransformed with constructs Z-F[1,2] and Z-F[3] was lysed by sonication, and a denaturing purification of the insoluble pellet undertaken using Ni-NTA (Qiagen) as described by the manufacturer. The proteins were eluted with a stepwise imidazole gradient. The fractions were analyzed by SDS-PAGE.
- mDHFR shares high sequence identity with the human DHFR (hDHFR) sequence. As the coordinates of the murine crystal structure were not available, we based our design considerations on the hDHFR structure.
- DHFR has been described as comprising three structural fragments forming two domains: the adenine binding domain (F[2]) and a discontinuous domain (F[1] and F[3]) 13,18 .
- the folate binding pocket and the NADPH binding groove are formed mainly by residues belonging to F[1] and F[2].
- Residues 101 to 108 of hDHFR form a disordered loop which lies on the same face of the protein as both termini. This loop occurs at the junction between F[2] and F[3].
- FIG. 2 illustrates the general features of the expressed constructs and the nomenclature used in this study.
- FIG. 3 panel A
- FIG. 3 panel A
- FIG. 3 illustrates the results of cotransformation of bacteria with constructs coding for Z-F[1,2] and Z-F[3], in the presence of trimethoprim, clearly showing that colony growth under selective pressure is possible only in cells expressing both fragments of mDHFR. There is no growth in the presence of either Z-F[1,2] or Z-F[3] alone. Induction of protein expression with IPTG is essential for colony growth ( FIG. 3A ).
- FIG. 4 (A) reveals the presence of the 1200 bp HincII restriction fragment from construct Z-F[1,2] as well as the 487 and 599 bp HincII restriction fragments from construct Z-F[3]. Also present is the 935 bp HincII fragment of pRep4.
- FIG. 4 (B) Overexpression of the fusion proteins is illustrated in FIG. 4 (B). In all cases, overexpression of a protein of the expected molecular weight is apparent on SDS-PAGE of the crude lysate. Purification of the coexpressed proteins under denaturing conditions yielded two bands of apparent homogeneity upon analysis by Coomassie-stained SDS-PAGE ( FIG. 4B ).
- FIG. 3 panel B illustrates the results of cotransformation of E. coli with construct Z-F[1,2] and the mutated Z-F[3] constructs.
- the doubling time measured for cells expressing Z-F[1,2]+Z-F[3], Z-F[1,2]+Z-F[3:Ile114Val] and Z-F[1,2]+Z-F[3:Ile114Ala] were 1.6-fold, 1.9-fold and 4.1-fold, higher respectively, than the doubling time of E. coli expressing pQE-16 in the absence of trimethoprim and IPTG.
- the presence of IPTG unexpectedly prevented growth of E. coli transformed with full-length mDHFR. Growth was partially restored by addition of the folate metabolism end-products thymine, adenine, pantothenate, glycine and methionine (data not shown). This suggests that induced overexpression of mDHFR was lethal to E. coli when grown in minimal medium as a result of depletion of the folate pool by binding to the enzyme.
- applicants make point mutations in the GCN4 leucine zipper of Z-F[1,2] and Z-F[3], for which direct equilibrium and kinetic parameters are known and correlating these known values with parameters derived from the PCA (Pelletier and Michnick, in preparation). Comparison of cell growth rates in this model system with rates for a DHFR PCA using unknowns would give an estimate of the strength of the unknown interaction. This should enable the determination of estimates of equilibrium and kinetic parameters for a specific protein-protein interaction.
- the present invention has illustrated and demonstrated a protein-fragment complementation assay (PCA) based on mDHFR, where a leucine zipper directs the reconstitution of DHFR activity. Activity was detected by an E. coli survival assay which is both practical and inexpensive.
- PCA protein-fragment complementation assay
- E. coli Aminoglycoside Kinase Optimization and Design of a PCA Using an Exonuclease-Molecular Evolution Strategy
- AK aminoglycoside kinase
- the optimization/selection procedure is as follows:
- Nested sets of deletions are created at the 5′ and the 3′ ends of the AK gene.
- unique restriction sites are introduced in the regions flanking the AK gene.
- an “outer” sticky site with a protruding 3′ terminus Sph I and Kpn I, respectively
- an “inner” sticky site with recessed 3′ terminus Bgl II and Sal I, respectively
- Cleavage at Sph I and Bgl II results in creation of a protruding terminus leading back to the flanking sequence and a recessed terminus leading into the AK gene. Digestion with E.
- coli exonuclease III and S1 nuclease yields a set of nested deletions from the recessed terminus only.
- 10 mg of DNA is digested with Sph I and Bgl II (or Kpn I and Sal I), phenol-chloroform extracted, and 12.5 U exonuclease III added.
- aliquots are taken and put into solution with 2 U S1 nuclease.
- the newly created ends are filled in with T4 DNA polymerase (0.1 Upper sample) and the set of vectors closed back by blunt-ended ligation (10 U ligase per sample).
- the average length of the deletion at each time point is determined by restriction analysis of the sets. This yields sets of AK genes deleted from the 5′ or the 3′ termini. This manipulation is undertaken directly in the pQE-32-Zipper constructs, such that the products can be used directly in activity screening.
- PCR is then performed with the fragmented DNA, using Taq polymerase (2.5 units/ul) in a PCR mixture containing 0.2 mM dNTPs, 2.2 mM Mg2Cl (or 0 mM for subuptimal PCR), 50 mM KCl, 10 mM Tris.HCl, pH 9.0, 0.1% TritonX-100.
- the primerless PCR product is then diluted 1:40 or 1:60 and used as template for PCR with 5′, 3′ complementary constant region oligos as primers for a further 20 cycles.
- Final product is restriction digested with Sph I and Kpn I and the products subcloned back into pQE32-Zipper to yield the final library of expression plasmids.
- E. coli BL21 cells are sequentially transformed with C-terminal or N-terminal complementary fragment-expression vectors at an estimated efficiency of 109 and finally cells cotransformed with the complementary fragment.
- E. coli are grown on agarose plates containing 1 ⁇ g/ml G418 and after 16 hours the largest colonies are selected and grown in liquid medium at increasing concentrations of G418.
- the enzyme structure is used to define minimal domains of the protein in question, as was done for DHFR.
- the enzyme structure is used to define minimal domains of the protein in question, as was done for DHFR.
- we select, during the exonuclease phase those fragments that at a minimum will code for one of the two domains.
- two well defined domains can be discerned in the structure consisting of residues 1-94 in the N-terminus and residues 95-267 in the C-terminus. Endonuclease digestions are performed as above, but reaction products are selected that will minimally code for one of the two domains.
- a further embodiment of the invention relates to PCA based on using heterodimeric or heteromultimeric enzymes in which the entire catalytic machinery is contained within one independently folding subunit and the other subunit provides stability and/or a cofactor to the enzymatic subunit.
- the regulatory subunit is split into complementary fragments and fused to interacting proteins. These fragments are co-transformed/transfected into cells along with the enzyme subunit.
- reconstitution and detection of enzyme activity is dependent on oligomerization domain-assisted reassembly of the regulatory subunit reassembly into its native topology. However, the reconstituted subunit then interacts with the intact enzymatic subunit to produce activity.
- the enzyme in this case is not a constitutive cellular enzyme, but rather an exogenous gene product.
- the enzyme can be expressed at higher levels than a natural gene and can also be modified to be directed to specific subcellular compartments (by subcloning compartment-specific signal peptides onto the N- or C-termini of the enzyme and subunit fragments).
- the specific advantage of this approach is that while the single enzyme strategy may lead to suboptimal enzymatic activity, in this approach, the enzyme folds independently and may in fact act as a chaperone to the fragmented regulatory subunit, aiding in its refolding.
- the Melc1 protein is split into two fragments that serve as the complementation part of the PCA. These fragments, fused to oligomerization domains, are coexpressed with Melc2, and the basis of the assay is that Melc2 activity is dependent on complementation of the Melc1 fragments.
- Stoichiometries of protein complexes can also be addressed (i.e. whether a complex consists of two or three proteins) as follows. One fuses two proteins to the two Melc1 fragments and a third to intact Melc2. It thus can be shown that the minimum complementary active complex of the tyrosinase will require that all three components and therefore a trimer is necessary.
- a key aspect of this approach is that we can easily demonstrate specific interactions by making one component, specifically the protein-Melc2 fusions catalytic subunit dependent on the other components by underexpressing it in the background of overexpressed Melc1 fragment-protein fusions.
- an alternate embodiments relates to PCAs based on the disruption of the interface between, for instance a dimeric enzyme that requires stable association of the subunits for catalytic activity.
- selective or random mutagenesis at the subunit interface would disrupt the interaction and the basis of the assay would be that oligomerization domains fused to the subunits would provide the necessary binding energy to bring the subunits together into a functional enzyme.
- PCA strategies listed thus far have used two-plasmid transformation strategies for expression of complementary fragments. This approach has some advantages, such as using different drug resistance markers to select for optimal incorporation of genes, for instance in transformed or transfected cells or for optimum transformation of complementary plasmids into bacteria and control of expression levels of PCA fragments using different promoters.
- single plasmid strategies have advantages in terms of simplicity of transfection/transformation. Protein expression levels can be controlled in different ways, while drug selection can be achieved in one of two ways: In the case of PCAs based on survival assay using enzymes that are drug resistance markers themselves, such as AK, or where the enzyme complements a metabolic pathway, such as DHFR, no additional drug resistance genes need be incorporated in the expression plasmids.
- PCA is based on an enzyme that produces a colored or fluorescent product, such as tyrosinase or firefly luciferase
- an additional drug resistance gene must be expressed from the plasmid.
- Expression of PCA complementary fragments and fused cDNA libraries/target genes can be assembled on single plasmids as individual operons under the control of separate inducible or constitutive promotors, or can be expressed polycistronically.
- E. coli polycistronic expression can be achieved using known interceding region sequences, for instance we use the region in the mel operon from which we derived the tyrosinase melc1-melc2 genes which we have shown to be expressed at high levels in E.
- differential control of the expression of one of the complementary fragment fusions can be controlled to minimize expression. This will be important in reducing background non-specific interactions. Examples of differential control of complementary fragment expression include the following strategies:
- the second gene in polycistronic expression, transient or stable, expression of the second gene will necessarily be less efficient and so this in itself could serve to limit the quantity of one of the complementary fragments.
- the first gene product can be limited in expression by mutation of an upstream donor/splice site, while the second gene can be put under the control of a retroviral internal initiation site, such as that of ECMV to enhance expression.
- Individual complementary fragment-fusion pairs can also be put under the control of inducible promoters, all commercially available including those based on Tet-responsive PhCMV*-1 promoter, and/or steroid receptor response elements. In such a system the two complementary fragment genes can be turned on and expression levels controlled by dose dependent expression with the inducer, in these cases tetracycline and steroid hormones.
- PCAs do not have these limitation since they do not require additional cellular machinery available only in specific compartments.
- a further point is that by performing the PCA strategy in appropriate model cell types, it is also possible to introduce appropriate positive and negative controls for studying a particular pathway. For instance, for a hormone signaling pathway it is likely that hormone signaling agonists and antagonists or dominant-negative mutants of signaling cascade proteins would be known, that are upstream or act in parallel to the events being examined in the PCA. These reagents could be used to determine if novel interactions detected by the PCA are biologically relevant. In general then, interactions that are detected only if hormone is introduced but are not seen if an antagonist is simultaneously introduced could be hypothesized to represent interactions relevant to the process under study.
- serine phosphorylation of the S6 protein of the 40S ribosomal subunit is the serine phosphorylation of the S6 protein of the 40S ribosomal subunit.
- the discovery of serine/threonine kinases that specifically phosphorylate S6 have considerably aided in identifying novel mitogen mediated signal transduction pathways.
- the serine/threonine kinase p70S6k has been identified as a specific S6 phosphorylase 131-136 .
- p70S6k is activated by serine and threonine phosphorylation at specific sites in response to several mitogenic signals including serum in serum starved cells, growth factors including insulin and PDGF, and by mitogens such as phorbol esters.
- D77-p70S6k-F[1,2] fusion and D77-p70S6k-F[3] fusion, or F[1,2] and D77-p70S6k-F[3] fusion will be cotransformed into E. coli and the cells grown in minimal medium in the presence of trimethoprim. Colonies will be selected and expanded for analysis of kinase activity against 40S ribosomal subunits, and for coexpression of the two proteins.
- coli BL21 harboring pRep4 for constitutive expression of the lac repressor; this is required in the case where a protein product is toxic to the cells
- a plasmid coding for the fusion p70S6k-D77-[1,2]; 3-plating on minimal medium in the presence of trimethoprim and IPTG; 4-selection of any colonies that grow, propagation and isolation of plasmid DNA, followed by sequencing of DNA inserts; 5-purification of unknown fusion products via the hexaHis-tag and sizing on SDS-PAGE.
- the first strand is then methylated.
- “EcoRI Adapters” are added, producing digestion of the linkers with EcoRI and XhoI (the inserts are protected by methylation) produces full-length cDNA ready for directional insertion in a vector opened with EcoRI and XhoI. Because the success of library screening depends largely on the quality of the cDNA produced, we will use the above methods as they have proven to consistently produce high-quality cDNA libraries.
- ii Insertion of the cDNA into vectors:
- the library will be constructed as a C-terminal fusion to mDHFR F[3] in vector pQE-32 (Qiagen), as we have obtained high levels of expression of mDHFR fusions from this vector in BL21 cells.
- Three such vectors will be created, differing at their 3′ end, which is the novel polycloning site that we engineered (described earlier, under Methods), carrying either 0, 1, or 2 additional nucleotides. This allows read-through from F[3] into the library fragments in all 3 translational reading frames.
- the cDNA fragments will be directionally inserted at the EcoRI and XhoI sites in all three vectors at once.
- the transformation of the system described above, is useful to produce an equivalent assay for use in eukaryotic cells.
- the basic principle of the assay is the same: the fragments of mDHFR are fused to associating domains, and domain association is detected by reconstitution of DHFR activity in eukaryotic cells ( FIG. 5 ).
- mDHFR reassembly Two systems can be used for detection of mDHFR reassembly, in parallel: i—CHO-DUKX B11 cells (Chinese Hamster Ovary cell line deficient in DHFR activity) are cotransfected with GCN4-zipper-mDHFR fragment fusions. The cells are grown in the absence of nucleotides; only cells carrying reconstituted DHFR will undergo normal cell division and colony formation. ii—Methotrexate (MTX)-resistant mutants of mDHFR have been created, with the goal of transfecting cells that have constitutive DHFR activity such as COS and 293 cells.
- MTX Metalhotrexate
- Mutants Z-F[1,2: Leu22Phe], Z-F[1,2: Leu22Arg] and Z-F[1,2: Phe31Ser] all allowed for bacterial survival with high growth rates when cotransformed with Z-F[3] (results not shown).
- the five mutants will be tested in eukaryotic cells, in reconstitution of mDHFR fragments to produce enzyme that can sustain COS or 293 cell growth while under the selective pressure of MTX, which will eliminate background due to activity of the native enzyme.
- the mutations offers an advantage in selection while presenting no apparent disadvantage with respect to reassembly of active enzyme.
- thymidylate will be added to the growth medium to partially relieve the selective pressure offered by the lack of nucleotides.
- mDHFR Leu22Phe mutant S6-F[1,2] and p70S6k-F[3], or F[1,2] and p70S6k-F[3] (as a control) will be cotransfected into COS cells and the cells will be serum starved for 48 hours followed by replating of cells at low density in serum and MTX. Colonies will be selected and expanded for analysis of kinase activity against 40S ribosomal subunits, and for coexpression of the two proteins. Further controls will be performed for inhibition of protein association with wortmannin and rapamycin.
- Steps 1 through 5 are parallel to those for the bacterial library screening (above).
- the library is constructed as a C-terminal fusion to mDHFR F[3].
- F[3] (with no stop codon) will be inserted in frame in Zap Express, followed by insertion of the novel polylinkers allowing expression of the inserts in all three reading frames (described above), and by the EcoRI/XhoI directional cDNA.
- This bacteriophage library will be propagated and treated with the Stratagene helper phage to excise a eukaryotic expression phagemid vector (pBK-CMV) carrying the fusion inserts.
- pBK-CMV eukaryotic expression phagemid vector
- the scheme for detecting partners for the protein p70S6k can be applied to studies of any biochemical pathway in any living organism. Such pathways may also be related to disease processes.
- the disease-related pathway may be an intrinsic process of cells in humans where a pathology arises from, for instance mutation, deletion or under or over expression of a gene.
- the biochemical pathway may be one that is specific to a pathogenic organism or the mechanism of host invasion.
- component proteins of such processes may be targets of a therapeutic strategy, such as development of drugs that inhibit invasion by the organism or a component enzyme in a biochemical pathway specific to the pathogenic organism.
- Inflamatory diseases are a case in point that can concern both examples.
- the protein-protein interactions that mediate the adhesion of leukocytes to inflamed tissues are known to involve such proteins as vascular cell adhesion molecule-1 (VCAM-1), and certain cytokines such as IL-6 and IL-8 that are produced during inflammation.
- VCAM-1 vascular cell adhesion molecule-1
- cytokines such as IL-6 and IL-8
- signaling pathways associated with inflammation such as those mediated by IL-1, IL-6, IL-8 and tumor necrosis have been studied in some detail and many direct and downstream regulators are known. These regulators can be used as starting point targets in a PCA screening to identify other signalling or modulating proteins that could also be targets for drug development.
- i the cellular mechanisms of inflammation as described above
- ii the discovery of the specific cell-surface ligands which the pathogenic organisms recognize and associate with.
- Secreted proteins produced by the pathogen can bind to the basolateral membrane of epithelial cells (as in the case in Yersinia pseudotuberculosis infection) or be translocated into intestinal epithelial cells ( Salmonella infection), promoting infectivity and/or physiological responses to the infection.
- Salmonella infection intestinal epithelial cells
- the interactions between the pathogenic protein and the epithelial cells are unknown.
- Cadherens are membrane proteins that mediates calcium dependent cell-cell adhesion. To do so they need another class of cytoplasmic proteins called cathenins. Those make a bridge between cadherins and cytoskeleton. Cathenins are also regulate genes that control differentiation-specific genes. For instance, the protein B-cathenin can interact in certain situation with a transcription factor (lef-1) and be translocated into the nucleus where it constrains the number of genes transactivated by lef-1 (differentiation).
- lef-1 transcription factor
- This process is regulated by the Wnt signaling pathway (homologs to the wingless pathway in drosophila ) by inactivation of GSK3B which permit degradation after of APC (a cytoplasmic adapter protein).
- PCA strategies could be used to identify novel proteins involved in the regulation of these processes.
- Proteins involved in viral integration processes are examples of targets that could be tested for inhibitors using the PCA strategies.
- Examples for the HIV virus include:
- Accessory proteins of HIV as a therapeutic target are identical to those of HIV.
- Vpr nuclear localizing sequence (target): interaction site of vpr with phosphatasesA.
- vif interaction with vimentin (cytoskeleton associated protein).
- Vpu Degradation of CD4 in the RE mediated by the cytoplasmic tail of Vpu.
- nef Myristoylation signal of Nef.
- Glutathione-S-Transferase (GST) GST from the flat worm Schistosoma japonicum is a small (28 kD), monomeric, soluble protein that can be expressed in both prokaryotic and eukaryotic cells.
- a high resolution crystal structure has been solved and serves as a starting point for design of a PCA.
- a simple and inexpensive colorimetric assay for GST activity has been developed consisting of the reductive conjugation of reduced glutathione with 1-chloro-2,4-dinitrobenzine (CNDB), a brilliant yellow product.
- CNDB 1-chloro-2,4-dinitrobenzine
- Cotransformants of zipper-GST-fragment fusions are expressed in E. coli on agar plates and colonies are transferred to nitrocellulose paper, Detection of fragment complementation is detected in an assay where a glutathione-CDNB reaction mixture is applied as an aerosol on the nitrocellulose and colonies expressing co-expressed fragments of GST are detected as yellow images.
- Green Fluorescent Protein (GFP) GFP from Aequorea victoria is becoming one of the most popular protein markers for gene expression. This is because the small, monomeric 238 amino-acids protein is intrinsically fluorescent due to the presence of an internal chromophore that results from the autocatalytic cyclization of the polypeptide backbone between residues Ser65 and Gly67 and oxidation of the — bond of Tyr66.
- the GFP chromophore absorbs light optimally at 395 nm and possesses also a second absorption maximum at 470 nm. This bi-specific absorption suggests the existence of two low energy conformers of the chromophore whose relative population depends on local environment of the chromophore.
- full-length GFP was fragmented by PCR into [F1] and [F2] fragments corresponding to amino acids 1-158 and 159-239 of the full length protein. Fragments were either amplified by PCR from pCMS-EGFP (Clontech) or were generated by oligonucleotide synthesis (Blue Heron, Wash.).
- the F[1]-GCN4 and GCN4-F[2] constructs consist of fusions with the GCN4 leucine zipper-forming sequences shown in FIG. 2 of the present specification.
- Mammalian expression vectors e.g.
- ATCC COS-7 cells were grown in DMEM (Life Technologies) supplemented with 10% fetal bovine serum (FBS, Hyclone).
- HEK293T cells were grown in MEM alpha medium (Invitrogen) supplemented with 10% FBS (Gemini Bio-Products), 1% penicillin, and 1% streptomycin and maintained in a 37° C. incubator at 5% CO2.
- FBS Femini Bio-Products
- penicillin 1%
- streptomycin 1% streptomycin
- Fire Fly Luciferase is a 62 kDa protein which catalyzes oxidation of the heterocycle luciferin. The product possesses one of the highest quantum yields for bioluminescent reactions: one photon is emitted for every oxidized luciferin molecule.
- the structure of luciferase has recently been solved, allowing for structure-based development of a PCA. As with our GST assay, cells are grown on a nitrocellulose matrix. The addition of the luciferin at the surface of the nitrocellulose permits it to diffuse across the cytoplasmic membranes and trigger the photoluminescent reaction. The detection is done immediately on a photographic film.
- Luciferase is an ideal candidate for a PCA: the detection assays are rapid, inexpensive, very sensitive, and utilizes non-radioactive substrate that is available commercially.
- the substrate of luciferase, luciferin can diffuse across the cytoplasmic membrane (under acidic pH), allowing the detection of luciferase in intact cells.
- This enzyme is currently utilized as a reporter gene in a variety of expression systems. The expression of this protein has been well characterized in bacterial, mammalian, and in plant cells, suggesting that it would provide a versatile PCA.
- XGPRT Xanthine-guanine phosphoribosyl transferase
- the E. coli enzyme XGPRT converts xanthine to xahthine monophosphate (XMP), a precursor of GMP.
- XMP xahthine monophosphate
- the mammalian enzyme hypoxanthine-guanine phosphoribosyl transferase HGPRT can only use hypoxanthine and guanine as substrates
- the bacterial XGPRT can be used as a dominant selection assay for a PCA for cells grown in the presense of xanthine.
- Vectors expressing XGPRT confer the ability of mammalian cells to grow in selective medium containing adenine, xanthine, and mycophenolic acid.
- the function of mycophenolic acid is to inhibit de novo synthesis of GMP by blocking the conversion of IMP into XMP (Chapman A. B., (1983) Molec. & Cellul. Biol. 3, 1421-1429).
- the only GMP produced then come from the conversion of xanthine into XMP, catalyzed by the bacterial XGPRT.
- aminoglycoside phosphotransferase fragments of XGPRT can be generated based on the known structure (See table 1.) using the design-evolution strategy described above with fragments fused to the GCN4 leucine zippers as a test oligomerization domains.
- the complementary fusions are cotransfected and the proteins transiently expressed in COS-7 cells, or stability expressed in CHO cells, grown in the selective medium.
- colonies are collected and sequentially re-cultured at increasing concentrations of the selective compounds in order to enrich for populations of cells that efficiently express the fusions at high concentrations.
- Adenosine deaminase (ADA) is present in minute quantities in virtually all mammalian cell. Although it is not an essential enzyme for cell growth, ADA can be used in a dominant selection assay. It is possible to establish growth conditions in which the cells require ADA to survive. ADA catalyzes the irreversible conversion of cytotoxic adenine nucleosides to their respective nontoxic inosine analogues. By adding cytotoxic concentrations of adenosine or cytotoxic adenosine analogues such as 9-b-D-xylofuranosyladenine to the cells, ADA is required for cell growth to detoxify the cytotoxic agent.
- ADA Cells that incorporate the ADA gene can then be selected for amplification in the presence of low concentrations of 2′-deoxycoformycin, a tight-binding transition state analogue inhibitor of ADA.
- ADA can then be used for a PCA based on cell survival (Kaufman, R. J. et al. (1986) Proc. of the Nat. Acad. Sci. (USA) 83, 3136-3140).
- fragments of ADA can be generated based on the known structure (See table 1.) using the design-evolution strategy described above with fragments fused to the GCN4 leucine zippers as a test oligomerization domains.
- the complementary fusions are cotransfected and the proteins transiently expressed in COS-7 cells, or stability expressed in CHO cells, grown in the selective medium containing 2′-deoxycoformycin.
- colonies are collected and sequentially re-cultured at increasing concentrations of 2′-deoxycoformycin in order to enrich for populations of cells that efficiently express the fusions at high concentrations
- Bleomycin binding protein (zeocin resistance gene) Zeocin, a member of the bleomycin/phleomycin family of antibiotics, is toxic to bacteria, fungi, plants, and mammalian cells. The expression of the zeocin resistance gene confers resistance to bleomycin/zeocin. The protein confers resistance by binding to and sequestering the drug and thus preventing its association and hydrolysis of DNA. Berdy, J. (1980) In Amino Acid and Peptide Antibiotics , J. Berdy, ed. (Boca Raton, Fla.: CRC Press), pp.
- Bleomycin binding protein could then be used for a PCA based on cell survival.
- BBP Bleomycin binding protein
- fragments of ADA can be generated based on the known structure (See table 1.) using the design-evolution strategy described above with fragments fused to the GCN4 leucine zippers as a test oligomerization domains.
- the BBP is a small (8 kD) dimer that binds to drugs via a subunit interface binding site.
- a single chain form of the dimer would be generated by making a fusion of two BBP genes with a short sequence coding for a simple polypeptide linker introduced between the two subunits. Fragments in this case will be based on a short sequence of one of the subunit modules, while the other fragment will be composed of the remaining sequence of the subunit plus the other subunit. Complementation and selection experiments will be performed as described for the examples above using bleomycin or zeocin as selective drugs.
- Hygromycin-B-phosphotransferase The antibiotic hygromycin-B is an aminocyclitol that inhibits protein Synthesis by disrupting translocation and promoting misreading.
- the E. coli enzyme hygromycin-B-phosphotransferase detoxifies the cells by phosphorylating Hygromycin-B. When expressed in mammalian cells, hygromycin-B-phosphotransferase can confer resistance to hygromycin-B (Gritz, L., and Davies, J. (1983) Gene 25, 179-188.). The enzyme is a dominant selectable marker and could be used for a PCA based on cell survival.
- L-histidinol NAD+oxydoreductase L-histidinol NAD+oxydoreductase.
- the hisD gene of Salmonella typhimurium codes for the L-histidinol NAD+oxydoreductase that converts histidinol to histidine.
- Mammalian cells grown in media lacking histidine but containing histidinol can be selected for expression of hisD (Hartman, S. C., R. C. Mulligan (1988) Proc. of the Nat. Acad. Sci. (USA) 85, 8047-8051).
- An additional advantage of using hisD in dominant selection is that histidinol is itself toxic, inhibiting the activity of endogenous histidyl-tRNA synthetase.
- Histidinol is also inexpensive and readily permeates cells.
- the structure of histidinol NAD+oxydoreductase is unknown and so development of a PCA based on this enzyme is based entirely on the exonuclease fragment/evolution strategy.
- the following Table list alternative embodiments using other PCA reporters.
- Abbreviations in Table: Type: D, dominant selection marker; R, recessive selection marker. Structure: four letter codes Protein Data Bank (PDB) entries; K, known but not deposited in PDB; U, unknown.
- mono/oligo M, monomer; D, dimer; tetra, tetramer.
- Thermophilic diaphorase 30 kD M NADH-diaphorase stain ( Bacillus stearothermophilus ) Glutathione-S-transferase 1GNE 26 kD D production of a yellow product by other the conjugation of glutathione with isoform an aromatic substance, chloro of dinitrobenzene (CDNB) 28 kD Luciferase 1LCI 62 kD M Fluorometric Green-fluorescent protein 1EMA 30 kD M Intrinsic fluorescence Chloramphenicol 1CLA 25 kD Tri Fluorimetric: Bodipy acetyltransferase chloramphenicol Uricase 32 kD Tetra Fluorometric SEAP (secreted form of human
- Multi-subunit PCA Two proteins need not interact for a PCA signal to be observed; if a partner protein or protein complex binds to two proteins simultaneously, it is possible to detect such a three protein complex.
- a multisubunit PCA is conceived with the example of herpes simplex virus thymidine kinase (TK), a homodimer of 40 kD.
- TK herpes simplex virus thymidine kinase
- the TK structure contains two well defined domains consisting of an alpha/beta (residues 1-223) and an alpha-helical domain (224-374).
- an alpha/beta residues 1-223
- an alpha-helical domain 224-374
- Rop1 dimer a four helix bundle homodimer.
- the two fragments of TK are extracted by PCR and subcloned into the transient transfection vector pMT3, the first in tandem to the Adenovirus major late promoter, tripartite leader 3′ to the first ATG, and the second downstream of a ECMV internal initiation site.
- Restriction sites previously introduced between the first and the last ATG are subcloned into BamHI/KpnI and PstI/EcoRI cloning sites downstream of the two ATGs. These are used to subclone PCR-generated fragments of the Rop1 subunits into two different vectors. Subsequently Ltk ⁇ cells are cotransfected by lipofection with the two plasmids and colonies of surviving cells are serially selected in medium containing increasing concentrations of HAT (hypoxanthine/aminopterin/thymidine). Cells that express complementary fragments of TK fused to the four Rop1 will proliferate under this selective pressure, or otherwise die. Specific examples of use of this concept would be in determining constituents of multiprotein complexes that are formed transiently or constitutively in cells.
- PCA is not limited to detecting protein-protein interactions, but can be adapted to detecting interactions of proteins with DNA, RNA, or small molecules.
- two proteins are fused to PCA complementary fragments, but the two proteins do not interact with each other.
- the interaction must be triggered by a third entity, which can be any molecule that will simultaneously bind to the two proteins or induce an interaction between the two proteins by causing a conformational change in one or both of the partners.
- a third entity which can be any molecule that will simultaneously bind to the two proteins or induce an interaction between the two proteins by causing a conformational change in one or both of the partners. Two examples have been demonstrated in our lab using the mDHFR PCA in E. coli .
- the immunosuppressant drug rapamycin is used to induce an interaction between its receptor FKBP12 and a partner protein mTOR (mammalian Target of Rapamycin).
- mTOR mammalian Target of Rapamycin
- trimethoprim as described above
- rapamycin rapamycin (0-10 nM).
- support of growth as detected by colony formation is completely dependent on the addition of rapamycin, suggesting that the mDHFR PCA is detecting a rapamycin-induced assembly of a FKBP12-mTOR and subsequent reconstitution of DHFR activity.
- Another example of an induced interaction we have studied with the DHFR PCA is the interaction of the oncogene GTPase p21 ras and its direct downstream target, the serine/threonine kinase raf. This interaction only occurs when the GTPase is in the GTP-bound form, whereas turnover of GTP to GDP leads to release of the complex. As with the FKBP-mTOR complex, we have demonstrated this induced interaction in E. coli . PCA could be used in a general way to study such induced interactions, and to screen for compounds that release or prevent these interactions in pathological states. The ras-raf interaction itself could be a target of therapeutic intervention.
- Oncogenic forms of ras consist of mutants that are incapable of turning over GTP and therefore remain continuously associated with activated ras. This leads to a constitutive uncontrolled growth signal that results, in part, in oncogenesis.
- the identification of compounds that inhibit this process, by PCA would be of value in broad treatment of cancers.
- Other examples of multimolecular applications of PCA could include identification of novel DNA or RNA binding proteins. In its simplest conception one uses a known DNA or RNA binding motifs, for instance a retinoic acid receptor zinc finger, or a simple RNA binding protein such as IF-1, respectively.
- One half of the PCA consists of the DNA or RNA protein binding domain fused to one of the PCA fragments (control fragment).
- a test system consists of tat/tar elements that control elongation in transcription/translation of HIV genes.
- An example application would be identification of tat binding elements that have been proposed to exist in eukaryotic genomes and may regulate genes in the same or similar way to that of HIV genes. (SenGupta D. J. et al. (1996) Proc. Natl. Acad. USA 6, 8496-8501).
- PCA Applications to Drug Screening Screening Combinatorial Libraries of Compounds for those that Inhibit or Induce Protein-Protein/Protein-rna/Protein-DNA Complexes
- proteins that interact/protein substrate pairs, or control DNA/RNA binding protein-enzyme pairs are fused to PCA complementary fragments and plasmids harboring these pairs are transformed/transfected into a cell, along with any third DNA or RNA element as the case requires.
- Transformed/transected cells are grown liquid culture in multiwell plates where each well is inoculated with a single compound from an array of combinatorially synthesized compounds. A readout of a response depends on the effect of a compound. If the compound inhibits a protein interaction, there is a negative response (no PCA signal is the positive response). If the compound induces a protein interaction, the response is a positive PCA signal.
- Controls for non-specific effects of compounds include: 1) demonstration that the compound does not effect the PCA enzyme itself (test against cells transfected with the wild-type intact enzyme used as the PCA probe) and in the case of a cell survival assay, that the compound is not toxic to the cells that have not been transformed/transfected.
- PCA also offers the advantage over in vitro assays that it is a test for cell membrane permeability of active compounds. Specific demonstrated examples of PCA for drug screening in our laboratory include the application of DHFR PCA in E. coli to detecting compounds that inhibit therapeutically relevant targets.
- a priming overnight culture is grown from frozen stocks in LB medium.
- a selective minimal medium containing trimethoprim, ampicillin, IPTG is aliquoted at 25 ml into each well of a 384 well plate.
- Each well is then inoculated with 1 ul of an individual sample from a compound array (ArQule Inc.) to give a final concentration of 10 uM.
- Each well is then inoculated with 2 ml of overnight culture and plates are incubated in a specially adapted shaker bath at 37 C. At 2 hour intervals, plates are read on an optical absorption spectroscopic plate reader coupled to a PC and spreadsheet software at 600 nm (scattering) for a period of 8 hours.
- Rates of growth are calculated from individual time readings for each well and compared to a standard curve.
- a “hit” is defined as a case where an individual compound reduces the rate of growth to less than the 95% confidence interval based on the standard deviation for growth rates observed in all of the wells within the test plate.
- Near hits are defined as those cases where growth rates are within the 95% confidence interval. For each of the hits or near hits, the following controls are then performed: The same experiment is performed with BL21 cells that are transformed with empty vector (and no trimethoprim), with vector harboring the full length mDHFR gene, or with cotransfected cells where protein expression is not induced by IPTG.
- the PCA strategy for compound screening can also be applied in the multiprotein protein-RNA/DNA cases as described above, and can easily be adapted to the DHFR or any other PCA in E. coli or in yeast versions of the same PCAs. Such screening can also be applied to enzymes whose targets are other proteins or nucleic acids for known enzyme/substrate pairs or to novel enzyme substrate pairs identified as described below.
- Proteins involved in viral integration processes are examples of targets that could be tested for inhibitors using the PCA strategies.
- Examples for the HIV virus include:
- Vpr nuclear localizing sequence (target): interaction site of vpr with phosphatasesA.
- vif interaction with vimentin (cytoskeleton associated protein).
- Vpu Degradation of CD4 in the RE mediated by the cytoplasmic tail of Vpu.
- nef Myristoylation signal of Nef.
- proteins linked neurodegenerative diseases such as to alpha-synuclein. This protein has been linked to early onset of Parkinson disease and it is present also implicated in Alzheimer disease. There is also ⁇ -amyloid proteins, linked to Alzheimers disease.
- protein-carbohydrate interactions that would be a target for drug screening includes the selecting that are generally implicated in inflammation. These cell surface glycoproteins are directly involved in diapedesis.
- tumor supressor genes whos actions are mediated by protein-protein interactions could be screened for potential anti-cancer compounds. These include PTEN, a tumor supressor directly involved in the formation of harmatomas. It is also involved in inherited breast and thyroid cancer. Other interesting tumor supressor genes include p53, Rb and BARC1.
- Enzymes may form tight complexes with their substrates (Kd ⁇ 1-10 ⁇ M). In these cases PCA may be efficient enough to detect such interactions. However, even if this is not true, PCA may work to detect weaker interactions. Generally, if the rate of catalysis and product release is slower than the rate of folding-reassembly of the PCA complementary fragments, effectively irreversible folding and reconstitution of the PCA reporter activity will have occurred. Therefore, even if the enzyme and substrate are no longer interacting, the PCA signal is detected. Therefore, the detection of novel enzyme substrates using PCA may be possible, independent of effective substrate Kd or rate of product release.
- AK aminoglycoside kinase
- the substrate trap mutant catalytic domain of PTP1B is fused to N-terminal complementary fragment of AK, while a C-terminal fusion of the other AK fragment is made to a cDNA library.
- Cells are co-transfected with complementary AK pairs and grown in selective concentrations of G418. After 72 hours, colonies of surviving cells are picked and in situ PCR is performed using primers designed to anneal to 3′ and 5′ flanking regions of the cDNA coding region. PCR amplified products are then 5′ sequenced to identify the gene.
- Enzyme inhibitors Screening combinatorial libraries of compounds for those that inhibit enzyme-PROTEIN substrate complexes either with:
- the PCA strategy can be used to generate peptides or proteins with novel binding properties that may have therapeutic value, as is done today with phage display technology. It is also possible to develop enzymes with novel substrate or physical properties for industrial enzyme development. Two detailed examples of the application of the PCA strategy to these ends are given below, with additional applications listed below.
- the designed zipper libraries expressed as N-terminal fusions to the DHFR F[1,2] or F[3:I114A], were cotransformed, clones were picked, propagated and mixed in selective liquid culture, and the mix was added in a 1:1 000 000 ratio to clone Z-F[1,2]+Z-F[3:I114A] (original GCN4 leucine zippers).
- the mixture was propagated in selective liquid culture over multiple passages. Restriction analysis shows that within 4 passages, the population of GCN4-expressing bacteria is diminishing relative to the novel zipper sequences (data not shown), indicating that some of the designed zipper-containing clones are propagated at a higher rate than those containing GCN4. Bacteria from later passages were plated on selective medium, and individual clones sequenced to reveal the identity of the most successful designed zipper pairs (data not shown).
- ADA Adenosine deaminase
- ADA is a small ( ⁇ 40 kD), and easily purified monomeric zinc metallo-enzyme and the structure of murine ADA has been resolved.
- Several in vitro ADA activity assays have been developed, involving UV spectrophotometry and stopped-flow fluorimetry.
- E. coli ADA catalyzes the irreversible conversion of cytotoxic adenine nucleosides to non-toxic inosines.
- the ADA gene has also been expressed in SF3834 E. coli cells which lack a gene coding for endogenous ADA. When the gene coding for ADA is introduced into ADA-bacterial DNA, those cells that express ADA are able to survive high concentrations of added adenosine; those that do not, die. This forms the basis of an in vivo ADA activity assay.
- ADA a dominant selective marker in mammalian and bacterial cells where the gene has been knocked out.
- the reason we choose dominant selective genes is because in screening for novel protein-protein interactions, particularly testing for interactions of a known protein against a library of millions of independent clones, selection serves to filter for cells that may show a positive response for reasons having nothing to do with a specific protein-protein interaction.
- the primary assay will be survival of SF3834 E. coli cells that have been transformed with the complementary ADA fragments fused to the test oligomerization proteins in the presense of toxic concentrations of adensosine. We will then purify fusion proteins from colonies of and perform in vitro assays of ADA activity as described below.
- ADA PCA as a method to identify novel proteins that interact with a test bait will be performed in mammalian COS-7 and HEK-293T cells transiently transfected with FKBP fused to one of the ADA fragments and the other fragment fused to a cDNA library from normal human spleen containing 10 6 independent clones.
- E. coli assay cells that survive in a medium containing toxic concentrations of ADA is collected and isolated plasmids will be tested to identify the gene for the interacting protein by PCR amplification and chain propagation-termination techniques.
- Structural motifs required for protein function Determination of the structural elements required for the enzymatic function of ADA are investigated through alteration of the structures of the enzyme fragments. At first, ADA is cut into two separate domains—one responsible for substrate binding (residues 1-210) and one responsible for catalysis (residues 211-352). These separate pieces will be attached to known assembly domains, such as leucine zippers (see example 1 above). Reassembly will restore activity which will be assessed through detailed in vitro kinetic analysis of the binding and catalytic properties of the re-assembled enzyme, using UV spectrophotometry and stopped-flow fluorimetry to observe the enzymatic reactions.
- This system will provide another handle on the manipulation of enzyme activity that will afford a powerful tool for enzymatic mechanism study.
- the difference in the kinetic behaviour of the reassembled enzyme on mixing with the substrate, compared to enzyme reassembled in presence of substrate (where substrate may already be bound by binding domain) will allow sophisticated level of study of importance of binding energy to catalysis.
- Subsequent point mutations to the functional or assembly domains of the proteins will then allow a very subtle perturbation and detailed quantification of the relationship of binding energy to catalysis.
- This precise control over the structure and assembly of separate functional domains of the enzyme will permit very sophisticated enzymatic structure function studies, the definition of structural motifs and an understanding of their role in catalysis.
- Novel protein catalyst design The detailed knowledge of the enzyme mechanism gained through determination of the structural requirements for catalysis will then be exploited through the combination of these functional “building blocks” with the functional motifs responsible for substrate binding and catalysis in other enzymes, allowing the generation of novel protein catalysts.
- the catalytic motif from ADA is modified to a cytidine-binding motif, creating a novel enzyme with potentially useful catalytic properties.
- the activity of these novel enzymes can easily be assessed through in vivo assays similar to that of the PCA system, or through in vitro activity assays.
- the detailed mechanistic investigation of the resulting enzymes possible with this system will permit the rational design of each subsequent generation of catalysts.
- PCAs were generated using mammalian expression vectors and cell lines stably expressing the PCAs were created and then implanted into mice.
- GFP fragments were generated by PCR from a mammalian codon-optimized version of GFP (pCMS-EGFP; Clontech).
- GFP[1] corresponded to amino acids 1 to 158 and GFP[2] to amino acids 159 to 239 of GFP.
- fragments encoding a variant of GFP (YFP PCA) were created by introducing the EYFP-specific mutations S65G, S72A into fragment 1 of GFP and the mutation T203Y into fragment 2 of GFP by PCR, resulting in fragments YFP[1] and YFP[2], respectively.
- the fragments YFP[1], and YFP[2] were subcloned into a mammalian expression vector (pcDNA3.1Z, Invitrogen), which had previously been modified to incorporate the replication origin (oriP) of the Epstein Barr virus (EBV).
- the oriP allows episomal replication of these modified vectors in cell lines expressing the EBNA1 gene, such as HEK293E cells (293-EBNA, Invitrogen). Additionally, these vectors still retain the SV40 origin, allowing for episomal expression in cell lines expressing the SV40 large T antigen (e.g. HEK293T, Jurkat or COS) as well.
- YFP[1] and YFP[2] fragments were amplified by PCR to incorporate restriction sites and a linker sequence, described below, in configurations that would allow fusion of a gene of interest to either the 5′- or 3′-end of each reporter fragment.
- the reporter-linker fragment cassettes were subcloned into a mammalian expression vector (pcDNA3.1Z, Invitrogen) that had been modified to incorporate the replication origin (oriP) of the Epstein Barr virus (EBV).
- the oriP allows episomal replication of these modified vectors in cell lines expressing the EBNA1 gene, such as HEK293E cells (293-EBNA, Invitrogen).
- these vectors still retain the SV40 origin, allowing for episomal expression in cell lines expressing the SV40 large T antigen (e.g. HEK293T, Tag-Jurkat or COS).
- the SV40 large T antigen e.g. HEK293T, Tag-Jurkat or COS.
- the integrity of the mutated reporter fragments and the new replication origin were confirmed by sequencing.
- PCAs were then created for pairs of full-length mammalian proteins that are known to interact in vivo (MEK/ERK and BAD/14-3-3sigma).
- the open reading frames of full-length rat mitogen-activated protein kinase 1 (MEK1), extracellular signal-regulated kinase 1 ERK1 (MAPK1), BAD and 14-3-3sigma were amplified by PCR from a sequence-verified full-length cDNA and then fused in-frame to complementary reporter fragments.
- Resulting PCR products were column purified (Centricon), digested with appropriate restriction enzymes to allow directional cloning, and fused in-frame to either the 5′ or 3′-end of YFP[1] or YFP[2] through a linker encoding a flexible 10 amino acid peptide (Gly.Gly.Gly.Gly.Ser)2.
- ERKERK1 was ligated to the 5′ end of YFP[1] while MEK1 was appended to YFP[2] in N-terminal fusions.
- BAD was fused 3′ of YFP[2] fragment and 14-3-3s was fused 5′ of the YFP[1] fragment.
- the fusion genes were subcloned into pcDNA3.1 expression vectors (Invitrogen) with Zeocin selectable marker for YFP[1]-fusions and hygromycin marker for YFP[2]fusions.
- Recombinants in the host strains DH5-alpha (Invitrogen, Carlsbad, Calif.) or XL1 Blue MR (Stratagene, La Jolla, Calif.) were screened by colony PCR, and clones containing inserts of the correct size were subjected to end sequencing to confirm the presence of the gene of interest and in-frame fusion to the appropriate reporter fragment.
- a subset of fusion constructs were selected for full-insert sequencing by primer walking.
- DNAs were isolated using Qiagen MaxiPrep kits (Qiagen, Chatsworth, Calif.). PCR was used to assess the integrity of each fusion construct, by combining the appropriate gene-specific primer with a reporter-specific primer to confirm that the correct gene-fusion was present and of the correct size with no internal deletions.
- HEK293T cells were grown in MEM alpha medium (Invitrogen) supplemented with 10% FBS (Gemini Bio-Products), 1% penicillin, and 1% streptomycin and maintained in a 37° C. incubator at 5% CO2.
- HEK293T cells were co-transfected with complementary PCA fragment pairs fused to full-length genes encoding proteins known to form interactions.
- Stable cell lines expressing the PCAs of interest were isolated following double antibiotic selection with 50 micrograms/ml Hygromycin B and 500 micrograms/ml Zeocin. The resulting fluorescence signals, generated by protein-fragment complementation, are shown in FIG. 10 .
- mice were injected subcutaneously, ventrally with a single dose of the human cells, as follows: (a) parental 293T cells; (b) cells stably expressing the Bad/14-3-3sigma PCA; and (c) cells stably expressing the MEK/ERK PCA.
- Each animal received 3 injections (one for each cell type) with 5 ⁇ 10exp6 cells per site. Fluorescence due to the PCA was immediately imaged with a whole-animal imaging system (AntiCancer Inc., San Diego, Calif.). Both of the PCA-engineered cells generated patches of fluorescence in live animals following injection (example shown in FIG. 11 ). All four animals showed clearly visible fluorescence at both the Bad/14-3-3 and MEK/ERK sites, and none at the parental cell site.
- the PCA strategy can be applied to transgenic organisms.
- the strategy can be used in conjunction with gene therapy, for example, to follow the results of gene introduction or of gene silencing. This strategy will also be useful in monitoring drug effects in live animals, in particular, in monitoring of the pharmacokinetics and pharmacodynamics of drug action or of gene therapy.
- Another important embodiment of the invention is to provide a means and method for gene therapy of mammalian disease.
- PCA therapeutic for treatment of cancer.
- a PCA is developed employing fragments (modular protein units) derived from a protein toxin for example: Pseudomonas exotoxin, Diptheria toxin and the plant toxin gelonin, or other like molecules.
- a mammalian, retroviral, adenoviral, or eukaryotic artificial chromosomal (EAC's) genetic construct is prepared that introduces one fragment of the selected toxin under the control of the promoter for expression of the erbB2 oncogene.
- the erbB2 oncogene is overexpressed in breast cancer and adenocarcinoma cells (D. J. Slamon et. al., Science, 1989, 244, 707).
- the HER2/neu (c-erbB-2) proto-oncogene encodes a sub-class 1 185-kDa transmembrane protein tyrosine kinase growth factor receptor, p185 HER2 .
- the human erbB2 oncogene is located on chromosome 17, region q21 and comprises 4,480 base pairs and p185 HER2 serves as a receptor for a 30-kDa glycoprotein growth factor secreted by human breast cancer cell lines (R. Lupu et. Al., Science, 1990, 249, 1152).
- transgene is introduced ‘in vivo’ or ‘ex-vivo’ into target cells employing methods known by those skilled in the art e.g. homologous recombination to insert transgene into locus of interest via retroviral, adenoviral or EAC's.
- a second genetic construct comprising a fusion gene containing a target DNA that encodes an interacting protein that interacts with erbB2 oncogene discovered by the PCA process described in this invention and the “second” fragment of the toxin molecule.
- This construct is delivered to the patient by methods known in the art for example as shown in U.S. Pat. Nos. 5,399,346 and 5,585,237 whose entire contents are incorporated by reference herein.
- Transgene expression of the erbB2 oncogene-toxin fragment described will now be under the control of the constitutive oncogene promoter. Proliferating tumor cells will thus produce one piece of the toxin attached as a fusion to the erbB2 oncogene.
- a second genetic construct expressing the PCA discovered interacting erbB2 oncogene “interacting protein-toxin fragment” construct then: erbB2 oncogene-toxin fragments: interacting protein-toxin fragment B will be created and induce death of target tumor cells through creation of an active toxin through Protein Fragment Complementation and thus provide an efficacious and efficient therapy of said disease.
- PCA strategies described above could be adapted to in vitro detection. Unlike the in vivo PCAs however, detection would be performed with purified PCA fragment-fusion proteins. Such uses of PCA have the potential for use in diagnostic kits. For example the test DHFR assay described above where the interacting domains are FKBP12 and TOR could be used as a diagnostic test for rapamycin concentrations for use in monitoring dosage in patients treated with this drug.
- the instant invention provides:
- the PCA method is particularly useful for detection of the kinetics of protein assembly in cells.
- the kinetics of protein assembly can be determined using fluorescent protein systems.
- PCA can be used for drug screening.
- the techniques of PCA are used to screen for drugs that block specific biochemical pathways in cells allowing for a carefully targeted and controlled method for identifying products that have useful pharmacological properties.
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Abstract
The instant invention describes a method for detecting protein-protein interactions in living organisms and/or cells, said method comprising: (a) synthesizing probe protein fragments from a protein which enables fluorescent or luminescent detection by dissecting the gene coding for the fluorescent or luminescent protein into a least two fragments; (2) constructing fusion proteins consisting of the probe protein fragments linked to protein domains that are to be tested for interactions; (3) coexpressing the fusion proteins; and (d) detecting reconstitution of the fluorescence or luminescence signal.
Description
- This application is a continuation-in-part of pending U.S. application Ser. No. 10/353,090 filed Jan. 29, 2003; which application is a continuation of pending U.S. application Ser. No. 10/154,758 filed May 24, 2002; which is a continuation of U.S. Ser. No. 09/499,464 filed Feb. 7, 2000; and now U.S. Pat. No. 6,428,951; which is a continuation of U.S. Ser. No. 09/017,412 filed Feb. 2, 1998; and now U.S. Pat. No. 6,270,964. The entire contents of all those patents and applications are incorporated by reference herein.
- The present invention relates to the determination of the function of novel gene products. The invention further relates to Protein fragment Complementation Assays (PCA). PCAs allow for the detection of a wide variety of types of protein-protein, protein-RNA, protein-DNA, Protein-carbohydrate or protein-small organic molecule interactions in different cellular contexts appropriate to the study of such interactions.
- Many processes in biology, including transcription, translation, and metabolic or signal transduction pathways, are mediated by non-covalently-associated multienzyme complexes1, 101. The formation of multiprotein or protein-nucleic acid complexes produce the most efficient chemical machinery. Much of modern biological research is concerned with identifying proteins involved in cellular processes, determining their functions and how, when, and where they interact with other proteins involved in specific pathways. Further, with rapid advances in genome sequencing projects there is a need to develop strategies to define “protein linkage maps”, detailed inventories of protein interactions that make up functional assemblies of proteins2,3. Despite the importance of understanding protein assembly in biological processes, there are few convenient methods for studying protein-protein interactions in vivo4,5. Approaches include the use of chemical crosslinking reagents and resonance energy transfer between dye-coupled proteins102, 103. A powerful and commonly used strategy, the yeast two-hybrid system, is used to identify novel protein-protein interactions and to examine the amino acid determinants of specific protein interactions4,6-8. The approach allows for rapid screening of a large number of clones, including cDNA libraries. Limitations of this technique include the fact that the interaction must occur in a specific context (the nucleus of S. cerevisiae), and generally cannot be used to distinguish induced versus constitutive interactions.
- Recently, a novel strategy for detecting protein-protein interactions has been demonstrated by Johnsson and Varshavsky108 called the ubiquitin-based split protein sensor (USPS)9. The strategy is based on cleavage of proteins with N-terminal fusions to ubiquitin by cytosolic proteases (ubiquitinases) that recognize its tertiary structure. The strategy depends on the reassembly of the tertiary structure of the protein ubiquitin from complementary N- and C-terminal fragments and crucially, on the augmention of this reassembly by oligomerization domains fused to these fragments. Reassembly is detected as specific proteolysis of the assembled product by cytosolic proteases (ubiquitinases). The authors demonstrated that a fusion of a reporter protein-ubiquitin C-terminal fragment could also be cleaved by ubiquitinases, but only if co-expressed with an N-terminal fragment of ubiquitin that was complementary to the C-terminal fragment. The reconstitution of observable ubiquitinase activity only occurred if the N- and C-terminal fragments were bound through GCN4 leucine zippers109,110. The authors suggested that this “split-gene” strategy could be used as an in vivo assay of protein-protein interactions and analysis of protein assembly kinetics in cells. Unfortunately, this strategy requires additional cellular factors (in this case ubiquitinases) and the detection method does not lend itself to high-throughput screening of cDNA libraries.
- Rossi, F., C. A. Charlton, and H. M. Blau (1997) Proc. Nat. Acad. Sci. (USA) 94, 8405-8410) have reported an assay based on the classical complementation of α and ω fragments of β-galactosidase (β-gal) and induction of complementation by induced oligomerization of the proteins FKBP12 and the mammalian target of rapamycin by rapamycin in transfected C2C12 myoblast cell lines. Reconstitution of b-gal activity is detected using substrate fluorescein di-β-D-galactopyranoside using several fluorescence detection assays. While this assay bears some resemblance to the present invention, there are several significant distinguishing differences. First, this particular complementation approach has been used for over thirty years in a vast number of applications including the detection of protein-protein interactions. Krevolin, M. and D. Kates (1993) U.S. Pat. No. 5,362,625) teaches the use of this complementation to detect protein-protein interactions. Also achievement of β-gal complementation in mammalian cells has previously been reported (Moosmann, P. and S. Rusconi (1996) Nucl. Acids Res. 24, 1171-1172). The individual PCAs presented here are completely de novo designed interaction detection assays, not described in any way previously except for publications arising from applicants laboratory. Secondly, this application describes a general strategy to develop molecular interaction assays from a large number of enzyme or protein detectors, all de novo designed assays, whereas the β-gal assay is not novel, nor are any general strategies or advancements over previously well documented applications given.
- As in the USPS, the yeast-two hybrid strategy requires additional cellular machinery for detection that exist only in specific cellular compartments. There is therefore a need for a detection system which uses the reconstitution of a specific enzyme activity from fragments as the assay itself, without the requirement for other proteins for the detection of the activity. Preferably, the assay would involve an oligomerization-assisted complementation of fragments of monomeric or multimeric enzymes that require no other proteins for the detection of their activity. Furthermore, if the structure of an enzyme were known it would be possible to design fragments of the enzyme to ensure that the reassembled fragments would be active and to introduce mutations to alter the stringency of detection of reassembly. However, knowledge of structure is not a prerequesite to the design of complementing fragments, as will be explained below. The flexibility allowed in the design of such an approach would make it applicable to situations where other detection systems may not be suitable.
- Recent advances in human genomics research has led to rapid progress in the identification of novel genes. In applications to biological and pharmaceutical research, there is now the pressing need to determine the functions of novel gene products; for example, for genes shown to be involved in disease phenotypes. It is in addressing questions of function where genomics-based pharmaceutical research becomes bogged down and there is now the need for advances in the development of simple and automatable functional assays. A first step in defining the function of a novel gene is to determine its interactions with other gene products in an appropriate context; that is, since proteins make specific interactions with other proteins or other biopolymers as part of functional assemblies, an appropriate way to examine the function of a novel gene is to determine its physical relationships with the products of other genes.
- Screening techniques for protein interactions, such as the yeast “two-hybrid” system, have transformed molecular biology, but can only be used to study specific types of constitutively interacting proteins or interactions of proteins with other molecules, in narrowly defined cellular and compartmental contexts and require a complex cellular machinery (transcription) to work. To rationally screen for protein interactions within the context of a specific problem requires more flexible approaches. Specifically, assays that meet criteria necessary not only to detecting molecular interactions, but also to validating these interactions as specific and biologically relevant.
- A list of assay characteristics that meet such criteria are as follows:
- 1). Allow for the detection of protein-protein, protein-DNA/RNA or protein-drug interactions in vivo or in vitro.
2) Allow for the detection of these interactions in appropriate contexts, such as within a specific organism, cell type, cellular compartment, or organelle.
3) Allow for the detection of induced versus constitutive protein-protein interactions (such as by a cell growth or inhibitory factor).
4) To be able to distinguish specific versus non-specific protein-protein interactions by controlling the sensitivity of the assay.
5) Allow for the detection of the kinetics of protein assembly in cells.
6) Allow for screening of cDNA, small organic molecule, or DNA or RNA libraries for molecular interactions. - The present invention seeks to provide the above-mentioned needs for which the prior art is silent. The present invention provides a general strategy for detecting protein interactions with other biopolymers including other proteins, nucleic acids, carbohydrates or for screening small molecule libraries for compounds of potential therapeutic value. In a preferred embodiment, the instant invention seeks to provide an oligomerization-assisted complementation of fragments of monomeric enzymes that require no other proteins for the detection of their activity. In one such embodiment, a protein-fragment complementation assay (PCA) based on reconstitution of dihydrofolate reductase activity by complementation of defined fragments of the enzyme in E. coli is hereby provided. This assay requires no additional endogenous factors for detecting specific protein-protein interactions (i.e. leucine zipper interactions) and can be conveniently extended to screening cDNA, nucleic acid, small molecule or protein design libraries for molecular interactions. In addition, the assay can also be adapted for detection of protein interactions in any cellular context or compartment and be used to distinguish between induced versus constitutive protein interactions in both prokaryotic and eukaryotic systems.
- One particular strategy for designing a protein complementation assay (PCA) is based on using the following characteristics: 1) A protein or enzyme that is relatively small and monomeric, 2) for which there is a large literature of structural and functional information, 3) for which simple assays exist for the reconstitution of the protein or activity of the enzyme, both in vivo and in vitro, and 4) for which overexpression in eukaryotic and prokaryotic cells has been demonstrated. If these criteria are met, the structure of the enzyme is used to decide the best position in the polypeptide chain to split the gene in two, based on the following criteria: 1) The fragments should result in subdomains of continuous polypeptide; that is, the resulting fragments will not disrupt the subdomain structure of the protein, 2) the catalytic and cofactor binding sites should all be contained in one fragment, and 3) resulting new N- and C-termini should be on the same face of the protein to avoid the need for long peptide linkers and allow for studies of orientation-dependence of protein binding.
- It should be understood that the above mentioned criteria do not all need to be satisfied for a proper working of the present invention. It is an advantage that the enzyme be small, preferably between 10-40 kDa. Although monomeric enzymes are preferred, multimeric enzymes can also be envisaged as within the scope of the present invention. The dimeric protein tyrosinase can be used in the instant assay. The information on the structure of the enzyme provides an additional advantage in designing the PCA, but is not necessary. Indeed, an additional strategy, to develop PCAs is presented, based on a combination of exonuclease digestion-generated protein fragments followed by directed protein evolution in application to the enzyme aminoglycoside kinase. Although the overexpression in prokaryotic cells is preferred it is not a necessity. It will be understood to the skilled artisan that the enzyme catalytic site (of the chosen enzyme) does not absolutely need to be on same molecule.
- The present application explains the rationale and criteria for using a particular enzyme in a PCA.
FIG. 1 shows a general description of a PCA. The gene for a protein or enzyme is rationally dissected into two or more fragments. Using molecular biology techniques, the chosen fragments are subcloned, and to the 5′ ends of each, proteins that either are known or thought to interact are fused. Co-transfection or transformation these DNA constructs into cells is then carried out. Reassembly of the probe protein or enzyme from its fragments is catalyzed by the binding of the test proteins to each other, and reconstitution is observed with some assay. It is crucial to understand that these assays will only work if the fused, interacting proteins catalyze the reassembly of the enzyme. That is, observation of reconstituted enzyme activity must be a measure of the interaction of the fused proteins. - A preferred embodiment of the present invention focuses on a PCA based on the enzyme dihydrofolate reductase. Expansion of the strategy to include assays in eukaryotic, cells, library screening, and a specific application to problems concerning the study of integrated biochemical pathways such as signal transduction pathways, is presented. Additional assays, including those based on enzymes that can act as dominant or recessive drug selection or metabolic salvage pathways are disclosed. In addition, PCAs based on enzymes that will produce a colored or fluorescent product are also disclosed. The present invention teaches how the PCA strategy can be both generalized and automated for functional testing of novel genes, screening of natural products or compound libraries for pharmacological activity and identification of novel gene products that interact with DNA, RNA or carbohydrates are disclosed. It also teaches how the PCA strategy can be applied to identifying natural products or small molecules from compound libraries of potential therapeutic value that can inhibit or activate such molecular interactions and how enzyme substrates and small molecule inhibitors of enzymes can be identified. Finally, it teaches how the PCA strategy can be used to perform protein engineering experiments that could lead to designed enzymes with industrial applications or peptides with biological activity.
- Simple strategies to design and implement assays for detecting protein interactions in vivo are disclosed herein. We have designed complementary fragments of the native mDHFR that, when coexpressed in E. coli grown in minimal medium, allow for survival of clones expressing the two fragments, where the basal activity of the endogenous bacterial DHFR is inhibited by the competitive inhibitor trimethoprim (
FIG. 3 ). Reconstitution of activity only occurred when both N- and C-terminal fragments of DHFR were coexpressed as C-terminal fusions to GCN4 leucine zipper sequences, indicating that reassembly of the fragments requires formation of a leucine zipper between the N- and C-terminal fusion peptides. The sequential increase in cell doubling times resulting from the destabilizing mutations directed at the assembly interface (Ile114 to Val, Ala or Gly) demonstrates that the observed cell survival under selective conditions is a result of the specific, leucine-zipper-assisted association of mDHFR fragment[1,2] with fragment[3], as opposed to nonspecific interactions of Z-F[3] with Z-F[1,2]. several detailed and many additional examples are given. - As demonstrated previously with the ubiquitin-based split protein sensor (USPS)9, a protein-fragment complementation strategy can be used to study equilibrium and kinetic aspects of protein-protein interactions in vivo. The DHFR and other PCAs however, are simpler assays. They are complete systems; no additional endogenous factors are necessary and the results of complementation are observed directly, with no further manipulation. The E. coli cell survival assay described herein should therefore be particularly useful for screening cDNA libraries for protein-protein interactions. mDHFR expression in cells can be monitored by binding of fluorescent high-affinity substrate analogues for DHFR26.
- There are several further aspects of the PCAs that distinguish them from all other strategies for studying protein-protein interactions in vivo (except USPS). We have designed complementary fragments of enzymes that allow for controlling the stringency of the assay, and could be used to obtain estimates of the kinetics and equilibrium constants for association of two proteins. For example, with DHFR the point mutations of the wild-type enzyme Ile 114 to Val, Ala, or Gly alter the stringency of reconstitution of DHFR activity. For determining estimates of equilibrium and kinetic parameters for a specific protein-protein interaction, one could perform a series of DHFR PCA experiments with two proteins that interact with a known affinity, using the wild type or destabilizing mutant DHFR fragments. Comparison of cell growth rates in this model system with rates for a DHFR PCA using unknowns would give an estimate of the strength of the unknown interaction.
- It should be understood that the present invention should not be limited to the DHFR or other PCAs presented, as it is only non-limiting embodiments of the protein complementation assay of the present invention. Moreover, the PCAs should not be limited in the context in which they could be used. Constructs could be designed for targeting the PCA fusions to specific compartments in the cell by addition of signaling peptide sequences27,28. Induced versus constitutive protein-protein interactions could be distinguished by a eukaryotic version of the PCA, in the case of an interaction that is triggered by a biochemical event. Also, the system could be adapted for use in screening for novel, induced protein-molecular associations between a target protein and an expression library.
- The instant invention is also directed to a method for detecting biomolecular interactions said method comprising:
- (a) selecting an appropriate reporter molecule;
- (b) effecting fragmentation of said reporter molecule such that said fragmentation results in reversible loss of reporter function;
- (c) fusing or attaching fragments of said reporter molecule separately to other molecules; followed by
- (d) reassociation of said reporter fragments through interactions of the molecules that are fused to said fragments.
- The invention also provides molecular fragment complementation assays for the detection of molecular interactions comprising a reassembly of separate fragments of a molecule, wherein reassembly of said fragments is operated by the interaction of molecular domains fused to each fragment of said molecules, and wherein reassembly of the fragments is independent of other molecular processes.
- In another aspect, the present invention is directed to a method of testing biomolecular interactions comprising:
- a) generating a first fusion product comprising
-
- i) a first fragment of a first molecule and
- ii) a second molecule which is different or the same as said first molecule;
- b) generating a second fusion product comprising
-
- i) a second fragment of said first molecule; and
- ii) a third molecule which is different from or the same as said first molecule or second molecule;
- c) allowing the first and second fusion products to contact each other; and
- d) testing for activity regained by association of the recombined fragments of the first molecule, wherein said reassociation is mediated by interaction of the second and third molecules.
- In another novel feature, the invention is directed to a method comprising an assay where fragments of a first molecule are fused to a second molecule and fragment association is detected by reconstitution of the first molecule's activity.
- The present invention also provides a composition comprising a product selected from the group consisting of:
- (a) a first fusion product comprising:
-
- 1) a first fragment of a first molecule whose fragments can exhibit a detectable activity when associated and
- 2) a second molecule that can bind (a)(1);
- (b) a second fusion product comprising
-
- 1) a second fragment of said first molecule and
- 2) a third molecule that can bind (b)(1); and
- c) both (a) and (b).
- The invention further provides a composition comprising complementary fragments of a first molecule, each fused to a separate fragment of a second molecule.
- The inventors of the present subject matter further provide a composition comprising a nucleic acid molecule coding for a fusion product, which molecule comprises sequences coding for a product selected from the group consisting of:
- (a) a first fusion product comprising:
-
- 1) fragments of a first molecule whose fragments can exhibit a detectable activity when associated and
- 2) a second molecule fused to the fragment of the first molecule;
- (b) a second fusion product comprising
-
- 1) a second fragment of said first molecule and
- 2) a second or third molecule; and
- (c) both (a) and (b).
- The present invention is also directed to a method of testing for biomolecular interactions associated with: (a) complementary fragments of a first molecule whose fragments can exhibit a detectable activity when associated or (b) binding of two protein-protein interacting domains from a second or third molecule, said method comprising:
- 1) creating a fusion of
-
- (a) a first fragment of a first molecule whose fragments can exhibit a detectable activity when associated and
- (b) a first protein-protein interacting domain;
- 2) creating a fusion of
-
- (a) a second fragment of said first molecule and
- (b) a second protein-protein interacting domain that can bind said first protein-protein interacting domain;
- 3) allowing the fusions of (1) and (2) to contact each other; and
- 4) testing for said activity.
- The instant invention further provides a composition comprising a product selected from the group consisting of:
- (a) a first fusion product comprising:
-
- 1) a first fragment of a molecule whose fragments can exhibit a detectable activity when associated and
- 2) a first protein-protein interacting domain;
- (b) a second fusion product comprising
-
- 1) a second fragment of said first molecule and
- 2) a second protein-protein interacting domain that can bind said first protein-protein interacting domain; and
- (c) both (a) and (b).
- The invention is also directed to a composition comprising a nucleic acid molecule coding for a fusion product, which molecule comprises sequences coding for either:
- (a) a first fusion product comprising:
-
- 1) a first fragment of a molecule whose fragments can exhibit a detectable activity when associated and
- 2) a first protein-protein interacting domain; or
- (b) a second fusion product comprising
-
- 1) a second fragment of said molecule and
- 2) a second protein-protein interacting domain that can bind said first protein-protein interacting domain; or
- (c) both (a) and (b).
- The invention also provides a method of detecting kinetics of protein assembly and screening cDNA libraries comprising performing PCA.
- In another embodiment, the invention further provides a method of testing the ability of a compound to inhibit molecular interactions in a PCA comprising performing a PCA in the presence of said compound and correlating any inhibition with said presence.
- In a further embodiment, the invention provides a method for detecting protein-protein interactions in living organisms and or cells, which method comprises:
- (a) synthesizing probe protein fragments from an enzyme which enables dominant selection by dissecting the gene coding for the enzyme into at least two fragments;
- (b) constructing fusion proteins with one or more molecules that are to be tested for interactions;
- (c) fusing the proteins obtained in (b) with one or more of the probe fragments;
- (d) coexpressing the fusion proteins; and
- (e) detecting the reconstitution of enzyme activity.
- The invention still provides a method for detecting biomolecular interactions said method comprising:
- (a) selecting an appropriate reporter molecule;
- (b) effecting fragmentation of said reporter molecule;
- (c) fusing or attaching fragments of said reporter molecule separately to other molecules; followed by (d) reassociation of said reporter fragments through interactions of the molecules that are fused to said fragments.
- Lastly, the invention also provides a novel method of affecting gene therapy, which includes the step of providing the assays and compositions described above.
- The present invention is pioneering as it is the first protein complementation assay displaying such a level of simplicity and versatility. The exemplified embodiments are protein-fragment complementation assays (PCA) based on mDHFR, where a leucine zipper directs the reconstitution of DHFR activity. Activity was detected by an E. coli survival assay which is both practical and inexpensive. This system illustrates the use of mDHFR fragment complementation in the detection of leucine zipper dimerization and could be applied to the detection of unknown, specific protein-molecular interactions in vivo.
- It should be understood that the instant invention is not limited to the PCAs presented here, as numerous other enzymes can be selected and used in accordance with the teachings of the present invention. Examples of such markers can be found in Kaufman, (1987 Genetic Eng. 9:155-198) and references found therein as well as table 1 of this application.
- It should also be clear to the skilled artisan to which the present invention pertains that the invention is not limited to the use of leucine zippers as the two interacting molecules. Indeed, numerous other types of protein-molecule interactions can be used and identified in accordance with the teaching of the present invention. The known types of motifs involved in protein-molecular interactions are well known in the art.
- With PCA, fragments of molecular reporters are created and are separately fused to interacting molecules. Upon interaction of the molecules of interest, the polypeptide reporter fragments come together, leading to folding and reconstitution of the reporter activity. Since virtually any useful reporter can be engineered for PCA, any readout can be generated, including cell survival under selective pressure; calorimetric; fluorescent; luminescent; or phosphorescent signals.
- This instant application describes the use of PCA in whole organisms or animals to characterize protein function and drug function in vivo (drug efficacy, and ADME (absorption, distribution, metabolism and excretion)); to elucidate the biology of cancer (including metastasis), infectious disease, immune responses, HIV, allergy, transplant rejection and other conditions; to develop and monitor gene therapy; and to study fundamental biological processes underlying development such as in the zebrafish or frog. Light-emitting PCAs are suitable for use with biophotonic imaging systems.
- PCA in whole animals can be performed either by starting with nucleic acids encoding the compositions of interest; or by starting with stable cell lines that have been created by transfection or co-transfection of the nucleic acid compositions of interest.
- Starting with DNA, nucleic acid compositions encoding genes of interest that have been tagged with PCA reporter fragments can be introduced into whole organisms either by injection or by the creation of transgenic animals. With transgenic animals, the gene/reporter construct(s) will be present in all cells in the body. With injection, specific organs can be targeted or the constructs can be injected into the bloodstream. For example, an engineered HIV virus can be created with reporter constructs in frame with sequences coding for HIV proteins, and injected directly into the bloodstream in order to study the process of viral infection and the effects of antiviral drugs.
- Starting with living cells, stable cell lines are created by standard methods involving transfection or co-transfection of the nucleic acid compositions of interest, followed by selective pressure to allow the growth of cells expressing or co-expressing the proteins of interest. In principle, any cell type and reporter can be used. The cells are then introduced into animals by one of several methods. In one embodiment, transformed cells are used in the nude mouse model to create mice with tumors comprised of the cells that express or co-express the fusion proteins of interest. In another embodiment, cells are grown intraperitoneally, in a manner similar to that used for the creation of monoclonal antibodies from hybridomas. These mice can be used to study ADME (absorption, distribution, metabolism and excretion) of drugs targeted against specific proteins. In a further embodiment, cells are injected into the bloodstream or into specific organs of interest in order to study biological processes such as extravasation, migration, metastasis, and tumor growth; and the effects of anticancer drugs. In addition, engineered bacteria can be injected into animals to study the infection process and the effects of antimicrobial agents. In vivo PCA will also provide for the study of immune responses, allergy, transplant rejection, and the effects of drugs or drug candidates on those processes.
- Finally, transgenic animals can be created that express a single fusion product or two complementary fusion products. With two complementary fusion products, every cell in the body would be expected to harbor the genes of interest, and individual cells or organs may express the complementary fusion products depending on the promoter under which the gene constructs are regulated. With a single fusion product, the complementary fusion product can be introduced into the transgenic animal by one of the methods above for introduction of nucleic acids. For example, this can be used to study viral/host protein-protein interactions by creating a transgenic animal in which the host protein of interest is tagged with the first fragment of the reporter, and the viral protein of interest is tagged with the second fragment of the reporter. Infection leads to expression of the tagged viral protein which, upon interaction with the complementary tagged host protein, generates a signal.
- Detection of PCA in vivo can be done by whole body imaging methods or by endoscopy. A variety of reporters can be used including those originally specified in U.S. Pat. No. 6,270,964 which provide for the generation of survival-selection, fluorescent, luminescent or phosphorescent signals. For example, using survival-selection PCA, cells can be created that will survive (in nude mice, or as ascites) only when two proteins interact. This can be done by creating PCA with an antibiotic resistance reporter gene such as hygromycin phosphotransferase, and feeding the animals antibiotics. If the protein-protein interaction of interest occurs the transplanted cells will survive, otherwise they will die. Alternatively, using a “cell-death PCA”, cells can be created that will survive only when a drug disrupts a protein-protein interaction.
- For light-emitting PCA applications, existing biophotonic imaging systems can be used to detect the signal. For example, Xenogen Corporation, see www.xenogen.com and references therein) sells imaging systems and such technologies would be suitable for use with PCA. For additional vendors of biophotonic systems see www.biophotonics-mag.com. It should be noted that biophotonic approaches have not previously been used with PCA; the molecular techniques previously used to generate a signal have only allowed the detection of unimolecular events such as the expression of a single tagged gene, or have used alternative technologies such as fluorescence resonance energy transfer (FRET). PCA allows the detection of biomolecular interactions using virtually any readout, and allows detection of the effects of agonists, antagonists and inhibitors on specific interactions, receptors, and pathways; responses of cells to cytokines; and interactions of pathogenic proteins with host proteins.
- Some applications of in vivo PCA are further described below, along with examples.
- Drug Efficacy/ADME
- Once a small molecule is developed against a specific molecular target (protein), clinical studies are needed to determine if the drug is effective in vivo. Key among those studies are pharmacokinetic evaluations of the absorption, distribution, metabolism and excretion of the drug. Such data are needed to determine if the compound persists in the animal for a sufficient period of time to allow therapeutic efficacy, and to establish the optimal dosage and frequency for administration of the drug. Pharmacokinetics usually requires sampling of blood and urine and quantitation of the administered agent, e.g. by mass spectroscopy.
- PCA can be used to eliminate the need for traditional ADME approaches, by using whole-body imaging to detect the effect of the pharmaceutical agent on the target within living tissues. For example, fluorescence PCA has been used to detect activation of the erythropoietin receptor in living cells in response to erythropoietin or a peptide mimetic, EMP1 (Remy and Michnick). Cells that stably express the EPO receptor/PCA fragment constructs can be introduced into animals as described under methods. Suitable PCA reporters include but are not limited to luciferase, GFP, and beta-lactamase. The small molecule EPO mimetics would then be administered to the mice. If the agent causes activation of the receptor, the reporter will reassemble from its complimentary fragments, leading to a detectable signal. Fluorescence or luminescence can then detected by whole body imaging. The signal will persist as long as the pharmaceutical agent is active. If the mimetic is rapidly degraded or excreted, the signal will diminish. This is a “live” approach to ADME that does not require serial sampling of body fluids, and eliminates the need for analysis of the compound itself within the body fluids thereby saving time and money. With in vivo PCA, ADME only requires serial imaging of the animal over a period of time to establish the time course of activity of the agent. Similar approaches can be applied to other biopharmaceuticals such as cytokines, hormones, and mimetics of these agents. In addition, in vivo PCA can be applied to studies of efficacy and pharmacokinetics of receptor antagonists e.g. Herceptin, and like compounds. Finally these approaches can be applied to any agent that blocks a signaling pathway or a biomolecular event within cells, by simply tagging the appropriate genes with the reporter fragments, creating cell lines, and establishing the cells in animals. Moreover, in vivo PCA can be applied by creating transgenic mice that express the fusion constructs in every cell line, thereby enabling studies of the distribution of the pharmaceutical agent within tissues.
- Cancer Biology
- In vivo studies of tumor cell behavior are critical to identifying novel targets and novel drugs for cancer therapeutics. Cells with the transformed phenotype are capable of growing in soft agar and can form tumors in nude mice. In addition, cells that have metastatic properties will, when injected into mice, extravasate (exit the blood stream) and take up residence in other tissues where they can establish metastatic disease. Each tumor type has a preferred site of metastasis which depends on blood flow from the primary organ and on the environment of the organ in which the cancer cell finds itself (seed vs. soil analogy). For example prostate cancer preferentially metastasizes to the bone, whereas breast cancer metastasizes to the liver and brain. Understanding the biology of cancer and the process of metastasis is key to the development of anticancer therapeutics since it is the metastases that ultimately kill the patient.
- In vivo PCA can be used to study cancer biology, to track cancer cells, and to develop new anticancer drugs against specific targets. Tumor cells stably expressing genes of interest are established in mice. For example, in survival-selection mode, cells stably expressing the Her-2/neu oncoprotein (receptor) together with reporter construct can be used to study the effects of Herceptin. A suitable PCA reporter, such as hygromycin phosphotransferase, can be fused to Her-2/neu genes to create cell lines that only survive under antibiotic treatment if the receptor is active (dimerized in active form). Herceptin would be expected to disrupt the association of the receptor leading to a reduction in tumor formation or size. Alternatively, with light-emitting PCAs, cells stably expressing the receptor constructs that serve as a biosensor for the presence of agonist/antagonist can be injected into animals. If tumors are formed they can be detected as light-emitting clusters of cells. The size and location of the tumor, and the intensity of the light, can be determined by imaging. Drugs which antagonize the receptor would diminish the signal. This application of PCA biosensors is not limited to receptors but can be applied to any biomolecular interaction that is of interest for cancer biology and anticancer drug development.
- Immunology/Transplant
- Applications of in vivo PCA to immunology include studies of transplant rejection, allergy, autoimmune disease, and AIDS.
- For example, the drug FK506 is a powerful immunophilin which has specific biochemical effects including competitive inhibition of the rapamycin-induced interaction of the FK506 binding protein (FKBP) and FRAP/TOR/RAFT. Remy and Michnick have shown that PCA can be used to study the pharmacokinetics of the association of these proteins and its inhibition by FK506. Immortalized cells stably expressing FKBP/TOR with PCA constructs can be introduced into mice (or transgenic mice can be created with the fusion constructgs). Administration of rapamycin to the animal would induce the association of FKBP/TOR leading to a light-emitting signal from the cells. FK506 would prevent the association of FKBP/TOR and would reduce the signal. Dose-responses of FK506 and similar agents could be established in this way.
- In vivo PCA could also be used to study infectious diseases and gene therapy.
- The present application refers to numerous prior art documents and the entire contents of all those prior art documents are herein incorporated by reference.
- Other features and advantages of the present invention will be apparent from the following description of the preferred embodiments thereof, the appended Examples and from the enjoined claims.
-
FIG. 1 provides a general description of a PCA. Using molecular biology techniques, the chosen fragments of the enzyme are subcloned, and to the 5′ ends of each, proteins that either are known or thought to interact are fused. Co-transfection or transformation these DNA constructs into cells is then carried out and reconstitution with some assay is observed. -
FIG. 2 is a scheme of the fusion constructs used in one of the embodiments of the invention. The hexahistidine peptide (6His), the homodimerizing GCN4 leucine zipper (Zipper) and mDHFR fragments (1, 2 and 3) are illustrated. The labels for the constructs are used to identify both the DNA constructs and the proteins expressed from these constructs. -
FIG. 3 : (A) shows E. coli survival assay on minimal medium plates. Control: Left side of the plate: E. coli harboring pQE-30 (no insert); right side: E. coli harboring pQE-16, coding for native mDHFR. Panel I: Left side of each plate: transformation with construct Z-F[1,2]; right side of each plate: transformation with construct Z-F[3]. Panel II: Cotransformation with constructs Z-F[1,2] and Z-F[3]. Panel III: Cotransformation with constructs Control-F[1,2] and Z-F[3]. All plates contain 0.5 mg/ml trimethoprim. In panels I to III, plates on the right side contain 1 mM IPTG. - (B) E. coli survival assay using destabilizing DHFR mutants. Panel I: Cotransformation of E. coli with constructs Z-F[1,2] and Z-F[3:Ile114Val]. Panel II: Cotransformation with Z-F[1,2] and Z-F[3:Ile114Ala]. Inset is a 5-fold enlargement of the right-side plate. Panel III: Cotransformation with Z-F[1,2] and Z-F[3:Ile114Gly]. All plates contain 0.5 mg/ml trimethoprim. Plates on the right side contain 1 mM IPTG.
-
FIG. 4 features the coexpression of mDHFR fragments. (A) Agarose gel analysis of restriction pattern resulting from HincII digestion of plasmid DNA.Lane 1 contains DNA isolated from E. coli cotransformed with constructs Z-F[1,2] and Z-F[3].Lanes - (B) SDS-PAGE analysis of mDHFR fragment expression.
Lanes 1 to 5 show crude lysate of untransformed E. coli (lane 1), or E. coli expressing Z-F[1,2] (20.8 kDa; lane 2), Z-F[3] (18.4 kDa; lane 3), Control-F[1,2] (14.2 kDa; lane 4), and Z-F[1,2]+Z-F[3] (lane 5).Lane 6 shows 40 ml out of 2 ml copurified Z-F[1,2] and Z-F[3]. Arrowheads point to the proteins of interest. Migration of molecular weight markers (in kDa) is indicated to the right. -
FIG. 5 illustrates the general features of a PCA based on a survival assay such as the DHFR PCA. The assay can be used in a bacterial or a mammalian context. The inserted target DNA can be a known sequence coding for a protein (or protein domain) of interest, or can be a cDNA library. -
FIG. 6 represents an autoradiograph of a COS cell lysate after a 30 min. 35S-Met-Cys pulse-labelling. The expression pattern is essentially identical to that observed in E. coli (seeFIG. 4 ). The DNA transfected into the cells (or cotransfected) is indicated above the respective lanes. -
FIG. 7 illustrates the results of a protein engineering application of the mDHFR bacterial PCA. Two semi-random leucine zipper libraries were created (as described in the text) and each inserted N-terminal to one of the mDHFR fragments. Cotransformation of the resulting zipper-DHFR fragment libraries in E. coli and plating on selective medium allowed for survival of clones harboring successfully interacting leucine zippers. Fourteen clones were isolated and the zippers were sequenced to identify the residues at the “e” and “g” positions. The “e-g” pairs were categorized, as having attractive pairing (charge:charge, charge:neutral polar or neutral polar:neutral polar) or repulsive pairing (charge:charge) and the number of each type of interaction scored for each clone. The total number of interactions for each clone is 6; the interactions are tallied on the histogram. -
FIG. 8 illustrates the construction of expression vectors for PCA with GFP fragments. -
FIG. 9 . shows the demonstration of a protein-fragment complementation assay based on green fluorescent protein (GFP). Homodimerization of GCN4 leucine zipper-forming sequences is shown in mammalian (COS-7) and human (HEK293T) cells. -
FIG. 10 illustrates PCA in human cell lines, showing the fluorescent signal generated by the interactions of two different pairs of full-length proteins. A mutant (yellow) version of GFP was used to generate the protein-fragment complementation assay. -
FIG. 11 describes the PCA strategy for the detection of molecular interactions in whole organisms. PCA in situ in a mouse xenograft model is demonstrated. - Other objects, advantages and features of the present invention will become more apparent upon reading of the following non-restrictive description of preferred embodiments with reference to the accompanying drawings which are exemplary and should not be interpreted as limiting the scope of the present invention.
- Selection of mDHFR for a PCA.
- In designing a protein-fragment complementation assay (PCA), we sought to identify an enzyme for which the following is true: 1) An enzyme that is relatively small and monomeric, 2) for which structural and functional information exists, 3) for which simple assays exist for both in vivo and in vitro measurement, and 4) for which overexpression in eukaryotic and prokaryotic cells has been demonstrated. Murine DHFR (mDHFR) meets all of the criteria for a PCA listed above. Prokaryotic and eukaryotic DHFR is central to cellular one-carbon metabolism and is absolutely required for cell survival in both prokaryotes and eukaryotes. Specifically it catalyses the reduction of dihydrofolate to tetrahydrofolate for use in transfer of one-carbon units required for biosynthesis of serine, methionine, purines and thymidylate. The DHFRs are small (17 kD to 21 kD), monomeric proteins. The crystal structures of DHFR from various bacterial and eukaryotic sources are known and substrate binding sites and active site residues have been determined111-114, allowing for rational design of protein fragments. The folding, catalysis, and kinetics of a number of DHFRs have been studied extensively115-119. The enzyme activity can be monitored in vitro by a simple spectrophotometric assay120, or in vivo by cell survival in cells grown in the absence of DHFR end products. DHFR is specifically inhibited by the anti-folate drug trimethoprim. As mammalian DHFR has a 12000-fold lower affinity for trimethoprim than does bacterial DHFR121, growth of bacteria expressing mDHFR in the presence of trimethoprim levels lethal to bacteria is an efficient means of selecting for reassembly of mDHFR fragments into active enzyme. High level expression of mDHFR has been demonstrated in transformed prokaryote or transfected eukaryotic cel s122-126.
- mDHFR shares high sequence identity with the human DHFR (hDHFR) sequence (91% identity) and is highly homologous to the E. coli enzyme (29% identity, 68% homology) and these sequences share visually superimposable tertiary structure111. Comparison of the crystal structures of mDHFR and hDHFR suggests that their active sites are essentially identical127,128. DHFR has been described as being formed of three structural fragments forming two domains129,130 the adenine binding domain (residues 47 to 105=fragment[2]) and a discontinuous domain (
residues 1 to 46=fragment[1] and 106 to 186 [3]; numbering according to the murine sequence). The folate binding pocket and the NADPH binding groove are formed mainly by residues belonging to fragments[1] and [2]. Fragment [3] is not directly implicated in catalysis. - Residues 101 to 108 of hDHFR, at the junction between fragment[2] and fragment[3], form a disordered loop which lies on the same face of the protein as both termini. We chose to cleave mDHFR between fragments [1,2] and [3], at residue 107, so as to cause minimal disruption of the active site and NADPH cofactor binding sites. The native N-terminus of mDHFR and the novel N-terminus created by cleavage occur on the same surface of the enzyme112, 128 allowing for ease of N-terminal covalent attachment of each fragment to associating fragments such as the leucine zippers used in this study. Using this system, we have obtained leucine-zipper assisted assembly of the mDHFR fragments into active enzyme.
- Mutagenic and sequencing oligonucleotides were purchased from Gibco BRL. Restriction endonucleases and DNA modifying enzymes were from Pharmacia and New England Biolabs. The mDHFR fragments carrying their own in-frame stop codon were subcloned into pQE-32 (Qiagen), downstream from and in-frame with the hexahistidine peptide and a GCN4 leucine zipper (
FIG. 1 ;FIG. 2 ). All final constructs were based on the Qiagen pQE series of vectors, which contain an inducible promoter-operator element (tac), a consensus ribosomal binding site, initiator codon and nucleotides coding for a hexahistidine peptide. Full-length mDHFR is expressed from pQE-16 (Qiagen). - Residues 235 to 281 of the GCN4 leucine zipper (a SalI/BamHI 254 bp fragment) were obtained from a yeast expression plasmid pRS3169. The recessed terminus at the BamHI site was filled-in with Klenow polymerase and the fragment was ligated to pQE-32 linearized with SalI/HindIII(filled-in). The product, construct Z, carries an open reading frame coding for the sequence Met-Arg-Gly-Ser followed by a hexahistidine tag and 13 residues preceding the GCN4 leucine zipper residues.
- The eukaryotic transient expression vector, pMT3 (derived from pMT2)16, was used as a template for PCR-generation of mDHFR containing the features allowing subcloning and separate expression of fragment[1,2] and fragment[3]. The megaprimer method of PCR mutagenesis29 was used to generate a full-length 590 bp product. Oligonucleotides complementary to the nucleotide sequence coding for the N- and C-termini of mDHFR and containing a novel BspEI site outside the coding sequence were used as well as an oligonucleotide used to create a novel stop codon after fragment[1,2], followed by a novel SpeI site for use in subcloning fragment[3].
- Complementary oligonucleotides containing the novel restriction sites: SnaBI, NheI, SpeI and BspEI, were hybridized together resulting in 5′ and 3′ overhangs complementary to EcoRI, and inserted into pMT3 at a unique EcoRI site. The 590 bp PCR product (described above) was digested with BspEI and inserted into pMT3 linearized at BspEI, yielding construct [1,2,3]. The 610 bp BspEI/EcoNI fragment (coding for DHFR fragment[1,2], followed by a novel stop and fragment[3] up to EcoNI) was filled in at EcoNI and subcloned into pMT3 opened with BspEI/HpaI, yielding construct F[1,2]. The 250 bp SpeI/BspEI fragment of construct [1,2,3] coding for DHFR fragment[3] (with no in-frame stop codon) was subcloned into pMT3 opened with the same enzymes. The stop codon of the wild-type DHFR sequence, downstream from fragment[3] in pMT3, was inserted as follows. Cleavage with EcoNI, present in both the inserted fragment[3] and the wild-type fragment[3], removal of the 683 bp intervening sequence and religation of the vector yielded a construct of fragment[3] with the wild-type stop codon, construct F[3].
- The 1051 bp and the 958 bp SnaBI/XbaI fragments of constructs F[1,2] and F[3], respectively, were subcloned into construct Z opened with BglII(filled-in)/NheI, yielding constructs Z-F[1,2] and Z-F[3] (
FIG. 2 ). For the Control expression construct, the 180 bp XmaI/BspEI fragment coding for the zipper was removed from construct Z-F[1,2], yielding construct Control-F[1,2] (FIG. 2 ). - Site-directed mutagenesis was performed30 to produce mutants at Ile14 (numbering of the wild-type mDHFR). The mutagenesis reaction was carried out on the KpnI/BamHI fragment of construct Z-F[3] subcloned into pBluescript SK+ (Stratagene), using oligonucleotides that encode a silent mutation producing a novel BamHI site. The 206 bp NheI/EcoNI fragment of putative mutants identified by restriction was subcloned back into Z-F[3]. The mutations were confirmed by DNA sequencing.
- E. coli Survival Assay
- E. coli strain BL21 carrying plasmid pRep4 (from Qiagen, for constitutive expression of the lac repressor) were made competent, transformed with the appropriate DNA constructs and washed twice with minimal medium before plating on minimal medium plates containing 50 mg/ml kanamycin, 100 mg/ml ampicillin and 0.5 mg/ml trimethoprim. One half of each transformation mixture was plated in the absence, and the second half in the presence, of 1 mM IPTG. All plates were placed at 37° C. for 66 hrs.
- E. coli Growth Curves
- Colonies obtained from cotransformation were propagated and used to inoculate 10 ml of minimal medium supplemented with ampicillin, kanamycin as well as IPTG (1 mM) and trimethoprim (1 μg/μl) where indicated. Cotransformants of Z-F[1,2]+Z-F[3:Ile114Gly] were obtained under non-selective conditions by plating the transformation mixture on L-agar (+kanamycin and ampicillin) and screening for the presence of the two constructs by restriction analysis. All growth curves were performed in triplicate. Aliquots were withdrawn periodically for measurement of optical density. Doubling time was calculated for early logarithmic growth (OD 600 between 0.02 and 0.2).
- Bacteria were propagated in Terrific Broth31 in the presence of the appropriate antibiotics to an OD600 of approximately 1.0. Expression was induced by addition of 1 mM IPTG and further incubation for 3 hrs. For analysis of crude extract, pellets from 150 ml of induced cells were lysed by boiling in loading dye. The lysates were clarified by microcentrifugation and analyzed by SDS-PAGE32. For protein purification, a cell pellet from 50 ml of induced E. coli cotransformed with constructs Z-F[1,2] and Z-F[3] was lysed by sonication, and a denaturing purification of the insoluble pellet undertaken using Ni-NTA (Qiagen) as described by the manufacturer. The proteins were eluted with a stepwise imidazole gradient. The fractions were analyzed by SDS-PAGE.
- Design of mDHFR Fragments for a PCA
- mDHFR shares high sequence identity with the human DHFR (hDHFR) sequence. As the coordinates of the murine crystal structure were not available, we based our design considerations on the hDHFR structure. DHFR has been described as comprising three structural fragments forming two domains: the adenine binding domain (F[2]) and a discontinuous domain (F[1] and F[3])13,18. The folate binding pocket and the NADPH binding groove are formed mainly by residues belonging to F[1] and F[2]. Residues 101 to 108 of hDHFR form a disordered loop which lies on the same face of the protein as both termini. This loop occurs at the junction between F[2] and F[3]. By cleaving mDHFR at residue 107, we created F[1,2] and F[3], thus causing minimal disruption of the active site and substrate binding sites. The native N-terminus of mDHFR and the novel N-terminus created by cleavage were covalently attached to the C-termini of GCN4 leucine zippers (
FIG. 1 ). - E. coli Survival Assays
-
FIG. 2 illustrates the general features of the expressed constructs and the nomenclature used in this study.FIG. 3 (panel A) illustrates the results of cotransformation of bacteria with constructs coding for Z-F[1,2] and Z-F[3], in the presence of trimethoprim, clearly showing that colony growth under selective pressure is possible only in cells expressing both fragments of mDHFR. There is no growth in the presence of either Z-F[1,2] or Z-F[3] alone. Induction of protein expression with IPTG is essential for colony growth (FIG. 3A ). The presence of the leucine zipper on both fragments of mDHFR is essential as illustrated by cotransformation of bacteria with both vectors coding for mDHFR fragments, only one of which carries a leucine zipper (FIG. 3A ). It should be noted that growth of control E. coli transformed with the full-length mDHFR is possible in the absence of IPTG due to low levels of expression in uninduced cells. - Confirmation of the presence of both plasmids in bacteria able to grow with trimethoprim was obtained from restriction analysis of the plasmid DNA purified from isolated colonies.
FIG. 4 (A) reveals the presence of the 1200 bp HincII restriction fragment from construct Z-F[1,2] as well as the 487 and 599 bp HincII restriction fragments from construct Z-F[3]. Also present is the 935 bp HincII fragment of pRep4. Overexpression of the fusion proteins is illustrated inFIG. 4 (B). In all cases, overexpression of a protein of the expected molecular weight is apparent on SDS-PAGE of the crude lysate. Purification of the coexpressed proteins under denaturing conditions yielded two bands of apparent homogeneity upon analysis by Coomassie-stained SDS-PAGE (FIG. 4B ). - Applicants generated mutants of F[3] to test whether reconstitution of mDHFR activity by fragment assembly was specific. Protein stability can be reduced by changing the side-chain volume in the hydrophobic core of a protein9,22-25. Residue Ile114 of mDHFR occurs in a core β-strand at the interface between F[1,2] and F[3], isolated from the active site. Ile 114 is in van der Waals contact with Ile51 and Leu93 in F[1,2]11. We mutated Ile 114 to Val, Ala, or Gly.
FIG. 3 (panel B) illustrates the results of cotransformation of E. coli with construct Z-F[1,2] and the mutated Z-F[3] constructs. The colonies obtained from cotransformation with Z-F[3:Ile114Ala] grew more slowly than those cotransformed with Z-F[3] or Z-F[3:Ile114Val] (see inset toFIG. 3B ). No colony growth was detected in cells cotransformed with Z-F[3:Ile114Gly]. The number of transformants obtained was not significantly different in the case where colonies were observed, implying that cells cotransformed with Z-F[1,2] and either Z-F[3], Z-F[3:Ile114Val] or Z-F[3:Ile114Ala] have an equal survival rate. Overexpression of the mutants Z-F[3:Ile114X] was in the same range as Z-F[3], as determined by Coomassie-stained SDS-PAGE (data not shown). - We also compared the relative efficiency of reassembly of mDHFR fragments by measuring the doubling time of the cotransformants in liquid medium. Doubling time in minimal medium was constant for all transformants (data not shown). Selective pressure by trimethoprim in the absence of IPTG prevented growth of E. coli except when transformed with pQE-16 coding for full-length DHFR due to low levels of expression in uninduced cells. Induction of mDHFR fragment expression with IPTG allowed survival of cotransformed cells (except in the case of Z-F[1,2]+Z-F[3:Ile114Gly], although the doubling times were significantly increased relative to growth in the absence of trimethoprim. The doubling time measured for cells expressing Z-F[1,2]+Z-F[3], Z-F[1,2]+Z-F[3:Ile114Val] and Z-F[1,2]+Z-F[3:Ile114Ala] were 1.6-fold, 1.9-fold and 4.1-fold, higher respectively, than the doubling time of E. coli expressing pQE-16 in the absence of trimethoprim and IPTG. The presence of IPTG unexpectedly prevented growth of E. coli transformed with full-length mDHFR. Growth was partially restored by addition of the folate metabolism end-products thymine, adenine, pantothenate, glycine and methionine (data not shown). This suggests that induced overexpression of mDHFR was lethal to E. coli when grown in minimal medium as a result of depletion of the folate pool by binding to the enzyme.
- In another embodiment, applicants make point mutations in the GCN4 leucine zipper of Z-F[1,2] and Z-F[3], for which direct equilibrium and kinetic parameters are known and correlating these known values with parameters derived from the PCA (Pelletier and Michnick, in preparation). Comparison of cell growth rates in this model system with rates for a DHFR PCA using unknowns would give an estimate of the strength of the unknown interaction. This should enable the determination of estimates of equilibrium and kinetic parameters for a specific protein-protein interaction.
- The present invention has illustrated and demonstrated a protein-fragment complementation assay (PCA) based on mDHFR, where a leucine zipper directs the reconstitution of DHFR activity. Activity was detected by an E. coli survival assay which is both practical and inexpensive. This system illustrates the use of mDHFR fragment complementation in the detection of leucine zipper dimerization and could be applied to the detection of unknown, specific protein-protein interactions in vivo.
- E. coli Aminoglycoside Kinase: Optimization and Design of a PCA Using an Exonuclease-Molecular Evolution Strategy
- Although applicants have demonstrated that the engineering/design strategy described above can be used to produce complementary enzyme fragments, it is obvious that proteins did not evolve in such a way that such fragments would be expected to have optimal physical characteristics, including solubility, foldability (fast folding), protease resistance, or enzymatic activity. An alternative embodiment to the engineering/design strategy is the endonuclease/evolution approach. This strategy can be used by itself or in conjunction with the engineering/design strategy. The advantages of this approach are that in principle, prior knowledge of the protein structure is not necessary, that the optimal fragments are chosen for PCA and that these fragments will also have optimal characteristics. Following selection of optimal complementary fragments, the fragments are exposed to multiple rounds of random mutagenesis. Mutagenesis is achieved by suboptimal PCR combined with chemical mutagenesis or DNA shuffling (Stemmer, W. P. C. (1994) Proc, Natl, Acad, Sci. USA 91, 10747-10751). The overall strategy is described for the case of aminoglycoside kinase (AK), an example of antibiotic resistance marker that can be used for dominant selection of prokaryotic cells such as E. coli or eukaryotic cells such as yeast or mammalian cell lines. The structure of an AK is already known, and so strategy (1) would be possible, however we chose to combine both strategy (1) as defined for DHFR above, in conjunction with strategy (2).
- The optimization/selection procedure is as follows:
- Nested sets of deletions are created at the 5′ and the 3′ ends of the AK gene. In order to create unidirectional deletions, unique restriction sites are introduced in the regions flanking the AK gene. At the 5′ and 3′ termini, an “outer” sticky site with a protruding 3′ terminus (Sph I and Kpn I, respectively) and an “inner” sticky site with recessed 3′ terminus (Bgl II and Sal I, respectively) are added by PCR. Cleavage at Sph I and Bgl II (or Kpn I and Sal I) results in creation of a protruding terminus leading back to the flanking sequence and a recessed terminus leading into the AK gene. Digestion with E. coli exonuclease III and S1 nuclease (Henikoff, S. (1987) Methods in Enzymology 155, 156-165) yields a set of nested deletions from the recessed terminus only. Thus, 10 mg of DNA is digested with Sph I and Bgl II (or Kpn I and Sal I), phenol-chloroform extracted, and 12.5 U exonuclease III added. At 30 sec intervals over 10 min, aliquots are taken and put into solution with 2 U S1 nuclease. The newly created ends are filled in with T4 DNA polymerase (0.1 Upper sample) and the set of vectors closed back by blunt-ended ligation (10 U ligase per sample). The average length of the deletion at each time point is determined by restriction analysis of the sets. This yields sets of AK genes deleted from the 5′ or the 3′ termini. This manipulation is undertaken directly in the pQE-32-Zipper constructs, such that the products can be used directly in activity screening.
- As a first step in determining the requirements for fragment complementation, we must determine the minimum N-terminal and C-terminal fragments of AK that, alone, are active. Sets of deletions are individually transformed into E. coli BL21 cells and expression of the AK fragments is induced by IPTG. The sets where a significant number of colonies appear in the presence of G418 serve to indicate the approximate length of N- and C-terminal AK fragments which retain activity. Fragment complementation must therefore be undertaken with fragments taken from within these limits. The zipper-directed fragment complementation is detected as follows: appropriate sets of deletions, or pools of sets, are cotransformed into BL21, expression is induced with IPTG and growth in the presence of varying G418 concentrations is monitored. Large colonies which grow in the presence of high G418 concentrations are selected as giving the most efficiently complementing products.
- Directed evolution of optimal AK fragments using “DNA shuffling After optimal fragments have been selected, the individual fragments are removed by restriction digestion at Sph I and Kpn I allowing for 5′ and 3′ constant priming regions flanking the N- or C-terminal complementary fragments of AK. These oligonucleotides (2-4 μg) are digested with DNaseI (0.005 units/ul, 100 ul) and fragments of 10-50 nucleotides are extracted from low melting point agarose. PCR is then performed with the fragmented DNA, using Taq polymerase (2.5 units/ul) in a PCR mixture containing 0.2 mM dNTPs, 2.2 mM Mg2Cl (or 0 mM for subuptimal PCR), 50 mM KCl, 10 mM Tris.HCl, pH 9.0, 0.1% TritonX-100. A PCR program of 94 C/60 sec.; 94 C 30 sec.; 55 C 30 sec.; 72 C 30 sec. times 30 to 50; 72
C 5 min. Samples are taken every 5 cycles after 25 cycles to monitor the appearance of reassembled complete fragments on agarose gel. The primerless PCR product is then diluted 1:40 or 1:60 and used as template for PCR with 5′, 3′ complementary constant region oligos as primers for a further 20 cycles. Final product is restriction digested with Sph I and Kpn I and the products subcloned back into pQE32-Zipper to yield the final library of expression plasmids. As before, E. coli BL21 cells are sequentially transformed with C-terminal or N-terminal complementary fragment-expression vectors at an estimated efficiency of 109 and finally cells cotransformed with the complementary fragment. E. coli are grown on agarose plates containing 1 μg/ml G418 and after 16 hours the largest colonies are selected and grown in liquid medium at increasing concentrations of G418. Those clones showing the maximal resistance to G418 are then selected and if maximum resistance or greater is reached the evolution is terminated. Otherwise the DNA shuffling procedure is repeated. Finally, optimal fragments are sequenced and physical properties and enzymatic activity are assessed. This optimized AK PCA is now ready to test for dominant selection in any other cell type including yeast and mammalian cell lines. This strategy can be used to develop any PCA based on enzymes that impart dominant or recessive selection to a drug or toxin or to enzymes that produce a colored or fluorescent product. In the later two cases the end point of the evolution process is at minimum, retainment of signal for the intact, wild type enzyme or enhancement of the signal. This strategy can also be used in the absence of knowledge of the enzyme structure, whether the enzyme in mono-, di- or multimeric structure. However, knowledge of the enzyme structure does not preclude applying this strategy as well, as described below. - As can be appreciated, knowledge of the enzyme structure can be used to render a more efficient way of using molecular evolution to design a PCA. In this case, the enzyme structure is used to define minimal domains of the protein in question, as was done for DHFR. Instead of generating fragments of completely random length for the N- or C-terminal fragments, we select, during the exonuclease phase, those fragments that at a minimum will code for one of the two domains. For instance, in the case of AK, two well defined domains can be discerned in the structure consisting of residues 1-94 in the N-terminus and residues 95-267 in the C-terminus. Endonuclease digestions are performed as above, but reaction products are selected that will minimally code for one of the two domains. These are then the starting points for fragment selection and evolution cycles as described above.
- A further embodiment of the invention relates to PCA based on using heterodimeric or heteromultimeric enzymes in which the entire catalytic machinery is contained within one independently folding subunit and the other subunit provides stability and/or a cofactor to the enzymatic subunit. In this embodiment of PCA, the regulatory subunit is split into complementary fragments and fused to interacting proteins. These fragments are co-transformed/transfected into cells along with the enzyme subunit. As with single enzyme PCA described for DHFR and AK, reconstitution and detection of enzyme activity is dependent on oligomerization domain-assisted reassembly of the regulatory subunit reassembly into its native topology. However, the reconstituted subunit then interacts with the intact enzymatic subunit to produce activity. This approach is reminiscent of the USPS system, except it has the advantage that the enzyme in this case is not a constitutive cellular enzyme, but rather an exogenous gene product. As such there is no problem with background activity from the host cell, the enzyme can be expressed at higher levels than a natural gene and can also be modified to be directed to specific subcellular compartments (by subcloning compartment-specific signal peptides onto the N- or C-termini of the enzyme and subunit fragments). The specific advantage of this approach is that while the single enzyme strategy may lead to suboptimal enzymatic activity, in this approach, the enzyme folds independently and may in fact act as a chaperone to the fragmented regulatory subunit, aiding in its refolding. In addition, folding of the fragments may need not be complete in order to impart regulation of the enzyme. This approach is realized by a colorimetric/fluorometric assay we have developed based on the Streptomyces tyrosinase. This enzyme catalyzes the conversion of tyrosine to deoxyphenylalanine (DOPA). The reaction can be measured by conversion of fluorocinyl-tyrosine to the DOPA form. The active enzyme consists of two subunits, the catalytic domain (Melc2) and a copper binding domain (Melc1). Melc1 is a small protein of 14 kD that is absolutely required for Melc2 activity. In the assay we are developing, the Melc1 protein is split into two fragments that serve as the complementation part of the PCA. These fragments, fused to oligomerization domains, are coexpressed with Melc2, and the basis of the assay is that Melc2 activity is dependent on complementation of the Melc1 fragments. Stoichiometries of protein complexes can also be addressed (i.e. whether a complex consists of two or three proteins) as follows. One fuses two proteins to the two Melc1 fragments and a third to intact Melc2. It thus can be shown that the minimum complementary active complex of the tyrosinase will require that all three components and therefore a trimer is necessary. A key aspect of this approach is that we can easily demonstrate specific interactions by making one component, specifically the protein-Melc2 fusions catalytic subunit dependent on the other components by underexpressing it in the background of overexpressed Melc1 fragment-protein fusions.
- Although applicants have described only fragment complementation of intact proteins, protein domains or subunits as comprising PCA, an alternate embodiments relates to PCAs based on the disruption of the interface between, for instance a dimeric enzyme that requires stable association of the subunits for catalytic activity. In such cases, selective or random mutagenesis at the subunit interface would disrupt the interaction and the basis of the assay would be that oligomerization domains fused to the subunits would provide the necessary binding energy to bring the subunits together into a functional enzyme.
- The PCA strategies listed thus far have used two-plasmid transformation strategies for expression of complementary fragments. This approach has some advantages, such as using different drug resistance markers to select for optimal incorporation of genes, for instance in transformed or transfected cells or for optimum transformation of complementary plasmids into bacteria and control of expression levels of PCA fragments using different promoters. However, single plasmid strategies have advantages in terms of simplicity of transfection/transformation. Protein expression levels can be controlled in different ways, while drug selection can be achieved in one of two ways: In the case of PCAs based on survival assay using enzymes that are drug resistance markers themselves, such as AK, or where the enzyme complements a metabolic pathway, such as DHFR, no additional drug resistance genes need be incorporated in the expression plasmids. If however the PCA is based on an enzyme that produces a colored or fluorescent product, such as tyrosinase or firefly luciferase, an additional drug resistance gene must be expressed from the plasmid. Expression of PCA complementary fragments and fused cDNA libraries/target genes can be assembled on single plasmids as individual operons under the control of separate inducible or constitutive promotors, or can be expressed polycistronically. In E. coli polycistronic expression can be achieved using known interceding region sequences, for instance we use the region in the mel operon from which we derived the tyrosinase melc1-melc2 genes which we have shown to be expressed at high levels in E. coli under the control of a strong (tac) promoter. Genes could also be expressed and induced off of independent promoters, such as tac and arabinose. For mammalian expression systems, single plasmid systems can be used for both transient or stable cell line expression and for constitutive or inducible expression. Further, differential control of the expression of one of the complementary fragment fusions, usually the bait-fused fragment, can be controlled to minimize expression. This will be important in reducing background non-specific interactions. Examples of differential control of complementary fragment expression include the following strategies:
- i) In polycistronic expression, transient or stable, expression of the second gene will necessarily be less efficient and so this in itself could serve to limit the quantity of one of the complementary fragments. Alternatively, the first gene product can be limited in expression by mutation of an upstream donor/splice site, while the second gene can be put under the control of a retroviral internal initiation site, such as that of ECMV to enhance expression.
ii) Individual complementary fragment-fusion pairs can also be put under the control of inducible promoters, all commercially available including those based on Tet-responsive PhCMV*-1 promoter, and/or steroid receptor response elements. In such a system the two complementary fragment genes can be turned on and expression levels controlled by dose dependent expression with the inducer, in these cases tetracycline and steroid hormones. - Among the greatest advantage of PCA over other molecular interaction screening methods is that they are designed to be performed both in vivo and in any type of cell. This feature is crucial if the goal of applying a technique is to identify novel interactions from libraries and simultaneously be able to determine if the interactions observed are biologically relevant. The detailed example given below, and other examples at the end of this section illustrate how it is that validation of interactions with PCA is possible. In essence, this is achieved as follows. In biochemical pathways, such as hormone receptor-mediated signaling, a cascade of enzyme-mediated chemical reactions are triggered by some molecular event, such as by hormone binding to its membrane surface receptor. Enzyme interactions with protein substrates and protein-protein or protein-nucleic acid interactions with enzyme-modified substrates then occur. Such biochemical signaling cascades only occur in specific cell types and model cell lines for studying these processes. Therefore, to detect induced interactions, such as with known proteins in a pathway with yet unidentified proteins, one obviously needs to perform such screening in appropriate model cell lines and in the correct cellular compartment. Only the PCA strategy can be used in a general way to do this. Protein-molecular interaction techniques such as yeast two- or three-hybrid techniques cannot be performed in a context where such events occur, except in the limiting case of nuclear interaction in yeast or interactions that are not triggered. There do exist mammalian two-hybrid techniques where it might be possible to detect induced protein interactions, but only again if the proteins involved can be simultaneously activated, transported to the nucleus and interact with their partners. PCAs do not have these limitation since they do not require additional cellular machinery available only in specific compartments. A further point is that by performing the PCA strategy in appropriate model cell types, it is also possible to introduce appropriate positive and negative controls for studying a particular pathway. For instance, for a hormone signaling pathway it is likely that hormone signaling agonists and antagonists or dominant-negative mutants of signaling cascade proteins would be known, that are upstream or act in parallel to the events being examined in the PCA. These reagents could be used to determine if novel interactions detected by the PCA are biologically relevant. In general then, interactions that are detected only if hormone is introduced but are not seen if an antagonist is simultaneously introduced could be hypothesized to represent interactions relevant to the process under study.
- Below is a detailed description of an application of the DHFR that illustrates these points, as well as further examples where the PCA strategy could be used.
- One of the earliest detectable events in growth factor-activated cell proliferation is the serine phosphorylation of the S6 protein of the 40S ribosomal subunit. The discovery of serine/threonine kinases that specifically phosphorylate S6 have considerably aided in identifying novel mitogen mediated signal transduction pathways. The serine/threonine kinase p70S6k has been identified as a specific S6 phosphorylase131-136. p70S6k is activated by serine and threonine phosphorylation at specific sites in response to several mitogenic signals including serum in serum starved cells, growth factors including insulin and PDGF, and by mitogens such as phorbol esters. Considerable effort has been made over the last five years to determine how p70/p85S6k are activated in response to mitogens. Two receptor-mediated pathways have been implicated in p70S6k activation, one associated with the phosphatidylinositol-3-kinase (PI(3)k) and the other with the PI(3)k homologue mTOR137-144. Key to understanding of this proposal, is the fact that the role of these enzymes in activation of p70S6k was determined by effects of two natural products on phosphorylation and enzyme activity: rapamycin, which indirectly inhibits mTOR activity, and wortmannin, which directly inhibits PI(3)k activity. It is also important to note that no direct upstream kinases or other regulatory proteins of p70S6k have been identified to this date.
- The interactions of p70S6k with its known substrate S6 can be studied as a test system for the DHFR PCA in E. coli and in mammalian cell lines. One can also seek to identify novel interactions with this enzyme that would lead to new insights into how this important enzyme is regulated. Also, since activation of the enzyme is mediated by multiple pathways that can be selectively inhibited with specific drugs, this is an ideal system to test PCAs as methods to distinguish induced versus constitutive protein-protein interactions.
- a) Testing of the E. coli Survival Assay: Interaction of p70S6k with S6
- This test is ideal, because the apparent Km (=250 nM) of p70S6k for S6 protein145 is approximately the same as the Kd for leucine zipper-forming peptides from GCN4 used in our test system. However, we will have to use a constitutively active form of the enzyme for our tests. An N-terminal truncated form of the enzyme D77-p70S6k, is constitutively active and will be used in these studies147.
- Methodology: D77-p70S6k-F[1,2] fusion and D77-p70S6k-F[3] fusion, or F[1,2] and D77-p70S6k-F[3] fusion (as a control) will be cotransformed into E. coli and the cells grown in minimal medium in the presence of trimethoprim. Colonies will be selected and expanded for analysis of kinase activity against 40S ribosomal subunits, and for coexpression of the two proteins.
- Screening an expression library for interactions with a given target (p70S6k-D77, in this case) will be straightforward in this system, given that the only steps involved are: 1-construction of the fusion-expression library as a fusion with mDHFR fragment[3]; 2-transformation of the library in E. coli BL21 harboring pRep4 (for constitutive expression of the lac repressor; this is required in the case where a protein product is toxic to the cells) and a plasmid coding for the fusion: p70S6k-D77-[1,2]; 3-plating on minimal medium in the presence of trimethoprim and IPTG; 4-selection of any colonies that grow, propagation and isolation of plasmid DNA, followed by sequencing of DNA inserts; 5-purification of unknown fusion products via the hexaHis-tag and sizing on SDS-PAGE.
- The overall strategy is illustrated in
FIG. 5 . 1-Construction of a directional fusion-expression library: i—cDNA production: One can isolate poly(A)+ RNA from BA/F3 cells (B-lymphoid cells) because these cells have successfully been used in the study of the rapamycin-sensitive p70S6k activation cascade130. To enrich for full-length mRNA, we will affinity purify the mRNA via the 5′ cap structure by the CAPture method148. Reverse transcription will be primed by a “Linker Primer”: it has a poly(T) tail to prime from the poly(A) mRNA tail, and an XhoI site for later use in directional subcloning of the fragments. The first strand is then methylated. After second strand synthesis and blunting of the products, “EcoRI Adapters” are added, producing digestion of the linkers with EcoRI and XhoI (the inserts are protected by methylation) produces full-length cDNA ready for directional insertion in a vector opened with EcoRI and XhoI. Because the success of library screening depends largely on the quality of the cDNA produced, we will use the above methods as they have proven to consistently produce high-quality cDNA libraries. ii—Insertion of the cDNA into vectors: The library will be constructed as a C-terminal fusion to mDHFR F[3] in vector pQE-32 (Qiagen), as we have obtained high levels of expression of mDHFR fusions from this vector in BL21 cells. Three such vectors will be created, differing at their 3′ end, which is the novel polycloning site that we engineered (described earlier, under Methods), carrying either 0, 1, or 2 additional nucleotides. This allows read-through from F[3] into the library fragments in all 3 translational reading frames. The cDNA fragments will be directionally inserted at the EcoRI and XhoI sites in all three vectors at once. 2, 3, 4, and 5—These steps have been described earlier, under Results, apart from the final sequencing of clones identified using sequencing primers specific to vector sequences flanking sites of library insertion. The protein purification will also be as described earlier, by a one-step purification on Ni-NTA (Qiagen). If the product size is more than 15 kDa over the molecular weight of the DHFR component (equal to a cDNA insert of more than 450 bp), we will have the inserts sequenced at the Sheldon Biotechnology Center (McGill University). - The transformation of the system described above, is useful to produce an equivalent assay for use in eukaryotic cells. The basic principle of the assay is the same: the fragments of mDHFR are fused to associating domains, and domain association is detected by reconstitution of DHFR activity in eukaryotic cells (
FIG. 5 ). - Creation of the expression constructs: The DNA fragments coding for the GCN4-zipper-mDHFR fragment fusions were inserted as one piece into pMT3, a eukaryotic transient expression vector126. Expression of the fusion proteins in COS cells was apparent on SDS-PAGE after 35[S]Met labeling.
- Survival assays in eukaryotic cells: Two systems can be used for detection of mDHFR reassembly, in parallel: i—CHO-DUKX B11 cells (Chinese Hamster Ovary cell line deficient in DHFR activity) are cotransfected with GCN4-zipper-mDHFR fragment fusions. The cells are grown in the absence of nucleotides; only cells carrying reconstituted DHFR will undergo normal cell division and colony formation. ii—Methotrexate (MTX)-resistant mutants of mDHFR have been created, with the goal of transfecting cells that have constitutive DHFR activity such as COS and 293 cells. We mutated F[1,2] in order to incorporate, one at a time, each of five mutations that significantly increase Ki (MTX): Gly15Trp, Leu22Phe, Leu22Arg, Phe31Ser and Phe34Ser (numbering according to the wild-type mDHFR sequence). These mutations occur at varying positions relative to the active site and relative to F[3], and have varying effects on Km (DHF), Km (NADPH) and Vmax of the full-length mammalian enzymes in which they were. Mutants Z-F[1,2: Leu22Phe], Z-F[1,2: Leu22Arg] and Z-F[1,2: Phe31Ser] all allowed for bacterial survival with high growth rates when cotransformed with Z-F[3] (results not shown). The five mutants will be tested in eukaryotic cells, in reconstitution of mDHFR fragments to produce enzyme that can sustain COS or 293 cell growth while under the selective pressure of MTX, which will eliminate background due to activity of the native enzyme. The mutations offers an advantage in selection while presenting no apparent disadvantage with respect to reassembly of active enzyme. If the reconstituted mDHFR produced in either of the survival assays allows eukaryotic cell growth that is significantly slower than growth with the wild-type enzyme, thymidylate will be added to the growth medium to partially relieve the selective pressure offered by the lack of nucleotides.
- It is necessary at the outset to test whether induced interactions with p70S6k can be detected. One can use the same test system as that for the E. coli test system described above: Induction of association of p70S6k with S6 protein.
- mDHFR Leu22Phe mutant S6-F[1,2] and p70S6k-F[3], or F[1,2] and p70S6k-F[3] (as a control) will be cotransfected into COS cells and the cells will be serum starved for 48 hours followed by replating of cells at low density in serum and MTX. Colonies will be selected and expanded for analysis of kinase activity against 40S ribosomal subunits, and for coexpression of the two proteins. Further controls will be performed for inhibition of protein association with wortmannin and rapamycin.
- An important part of the work required in creating a library for use in eukaryotic cells will have been accomplished already, as the EcoRI/XhoI directional cDNA produced by the Stratagene “cDNA Synthesis Kit” can directly be inserted directionally into the Stratagene Zap Express vector.
-
Steps 1 through 5 are parallel to those for the bacterial library screening (above). 1—Again, the library is constructed as a C-terminal fusion to mDHFR F[3]. F[3] (with no stop codon) will be inserted in frame in Zap Express, followed by insertion of the novel polylinkers allowing expression of the inserts in all three reading frames (described above), and by the EcoRI/XhoI directional cDNA. This bacteriophage library will be propagated and treated with the Stratagene helper phage to excise a eukaryotic expression phagemid vector (pBK-CMV) carrying the fusion inserts. 2—Cotransfection of the library and p70S6k-F[1,2] constructs in eukaryotic cells: we will perform the screening in COS or 293 cells, as these are responsive to serum in activating the p70S6k signaling pathway. Selection experiments will be performed as described for the S6 test system above. 3—Propagation, isolation and sequencing of the insert DNA will be undertaken. 4—The cloned fusion proteins will be sized on SDS-PAGE by direct visualization after 35S-Met/Cys labeling, or by Western blotting using a commercial polyclonal antibody to mDHFR. - Generalization of the Strategy: The scheme for detecting partners for the protein p70S6k can be applied to studies of any biochemical pathway in any living organism. Such pathways may also be related to disease processes. The disease-related pathway may be an intrinsic process of cells in humans where a pathology arises from, for instance mutation, deletion or under or over expression of a gene. Alternatively the biochemical pathway may be one that is specific to a pathogenic organism or the mechanism of host invasion. In this case, component proteins of such processes may be targets of a therapeutic strategy, such as development of drugs that inhibit invasion by the organism or a component enzyme in a biochemical pathway specific to the pathogenic organism.
- Inflamatory diseases are a case in point that can concern both examples. The protein-protein interactions that mediate the adhesion of leukocytes to inflamed tissues are known to involve such proteins as vascular cell adhesion molecule-1 (VCAM-1), and certain cytokines such as IL-6 and IL-8 that are produced during inflammation. However, many of the proteins involved in onset of inflammatory response remain unknown; further, the intracellular signaling pathways triggered by the extracellular associations are poorly understood. The PCAs could be used in elucidation of the mechanisms underlying the onset of inflammation, as well the ensuing signaling. For example, signaling pathways associated with inflammation, such as those mediated by IL-1, IL-6, IL-8 and tumor necrosis have been studied in some detail and many direct and downstream regulators are known. These regulators can be used as starting point targets in a PCA screening to identify other signalling or modulating proteins that could also be targets for drug development.
- There is an increased risk of infection by enteric pathogens in the occurrence of the intestinal inflammation that characterizes idiopathic intestinal diseases. There are two mechanisms which need to be better understood here and which can be addressed by PCA:
- i—the cellular mechanisms of inflammation as described above, and
ii—the discovery of the specific cell-surface ligands which the pathogenic organisms recognize and associate with. Secreted proteins produced by the pathogen can bind to the basolateral membrane of epithelial cells (as in the case in Yersinia pseudotuberculosis infection) or be translocated into intestinal epithelial cells (Salmonella infection), promoting infectivity and/or physiological responses to the infection. However, in most cases the interactions between the pathogenic protein and the epithelial cells are unknown. - Cell adhesion and nervous system regeneration A related example in cell adhesion includes processes involved in development and regeneration in the nervous system. Cadherens are membrane proteins that mediates calcium dependent cell-cell adhesion. To do so they need another class of cytoplasmic proteins called cathenins. Those make a bridge between cadherins and cytoskeleton. Cathenins are also regulate genes that control differentiation-specific genes. For instance, the protein B-cathenin can interact in certain situation with a transcription factor (lef-1) and be translocated into the nucleus where it constrains the number of genes transactivated by lef-1 (differentiation). This process is regulated by the Wnt signaling pathway (homologs to the wingless pathway in drosophila) by inactivation of GSK3B which permit degradation after of APC (a cytoplasmic adapter protein). PCA strategies could be used to identify novel proteins involved in the regulation of these processes.
- Proteins involved in viral integration processes are examples of targets that could be tested for inhibitors using the PCA strategies. Examples for the HIV virus include:
- i) inhibition of integrase or the transport of the pre-integration complex: protein Ma or vpr.
ii) Inhibition of the cell cycle in G2 by vpr (interaction by cyclin B) causing induction of apoptosis.
iii) Inhibition of the interaction of gp160 (precursor of the membrane proteins) with furine. - Accessory proteins of HIV as a therapeutic target:
- i) Vpr: nuclear localizing sequence (target): interaction site of vpr with phosphatasesA.
ii) vif: interaction with vimentin (cytoskeleton associated protein).
ii) Vpu: Degradation of CD4 in the RE mediated by the cytoplasmic tail of Vpu.
iii) nef: Myristoylation signal of Nef. - Other examples of assays are herein exemplified. The reason to produce these assays is to provide alternative PCA strategies that would be appropriate for specific protein association problems such as studying equilibrium or kinetic aspects of assembly. Also, it is possible that in certain contexts (for example, specific cell types) or for certain applications, a specific PCA will not work but an alternative one will. Further below are brief descriptions of each other PCAs embodiments.
- 1) Glutathione-S-Transferase (GST) GST from the flat worm Schistosoma japonicum is a small (28 kD), monomeric, soluble protein that can be expressed in both prokaryotic and eukaryotic cells. A high resolution crystal structure has been solved and serves as a starting point for design of a PCA. A simple and inexpensive colorimetric assay for GST activity has been developed consisting of the reductive conjugation of reduced glutathione with 1-chloro-2,4-dinitrobenzine (CNDB), a brilliant yellow product. We have designed a PCA based on similar structural criteria used to develop the DHFR PCA using GCN4 leucine zippers as oligomerization domains. Cotransformants of zipper-GST-fragment fusions are expressed in E. coli on agar plates and colonies are transferred to nitrocellulose paper, Detection of fragment complementation is detected in an assay where a glutathione-CDNB reaction mixture is applied as an aerosol on the nitrocellulose and colonies expressing co-expressed fragments of GST are detected as yellow images.
- 2) Green Fluorescent Protein (GFP) GFP from Aequorea victoria is becoming one of the most popular protein markers for gene expression. This is because the small, monomeric 238 amino-acids protein is intrinsically fluorescent due to the presence of an internal chromophore that results from the autocatalytic cyclization of the polypeptide backbone between residues Ser65 and Gly67 and oxidation of the — bond of Tyr66. The GFP chromophore absorbs light optimally at 395 nm and possesses also a second absorption maximum at 470 nm. This bi-specific absorption suggests the existence of two low energy conformers of the chromophore whose relative population depends on local environment of the chromophore. A mutant Ser65Thr that eliminates isomerization (single absorption maximum at 488 nm) results in a 4 to 6 times more intense fluorescence than the wild type. Recently the structure of GFP has been solved by two groups, making it now a candidate for a structure-based PCA-design, which we have begun to develop.
- To demonstrate the construction of a green fluorescent protein PCA, full-length GFP was fragmented by PCR into [F1] and [F2] fragments corresponding to amino acids 1-158 and 159-239 of the full length protein. Fragments were either amplified by PCR from pCMS-EGFP (Clontech) or were generated by oligonucleotide synthesis (Blue Heron, Wash.). The F[1]-GCN4 and GCN4-F[2] constructs consist of fusions with the GCN4 leucine zipper-forming sequences shown in
FIG. 2 of the present specification. Mammalian expression vectors (e.g. commercially available pcDNA3.1) utilizing a standard CMV promoter, a BGH polyadenylation site (BGH pA), and selectable markers for expression in E. coli and mammalian cells were used as the ‘backbone’ for constructing the GFP PCA vectors (FIG. 8 ). In all cases, a 10 amino acid flexible linker consisting of (Gly.Gly.Gly.Gly.Ser)2 was inserted between the cDNA and the GFP fragments as described above for the DHFR PCA. - ATCC COS-7 cells were grown in DMEM (Life Technologies) supplemented with 10% fetal bovine serum (FBS, Hyclone). HEK293T cells were grown in MEM alpha medium (Invitrogen) supplemented with 10% FBS (Gemini Bio-Products), 1% penicillin, and 1% streptomycin and maintained in a 37° C. incubator at 5% CO2. On Day 1: Cells were seeded at 20,000 cells/well in poly-lysine coated 24- or 48-well dishes. On Day 2: Cells were transfected with 150 ng of each plasmid, using Fugene transfection reagent (Roche Diagnostics, Indianapolis, Ind.) as per the manufacturer's recommendations. Following 24 hrs of expression, cells were washed once with PBS and viewed on a Nikon TE-2000 microscope equipped with a HYQ-FITC filter cube (excitation: 460-500 nm; emission: 505-560 nm; dichroic mirror: 505LP). Images were acquired with a CoolSnap HQ CCD camera. The resulting fluorescence was visualized by microscopy (
FIG. 9 ). Fluorescence generated by dimerization of the leucine zippers could easily be detected in both cell types, demonstrating successful reconstitution of GFP activity as a result of fragment complementation. - 3) Fire Fly Luciferase. Firefly luciferase is a 62 kDa protein which catalyzes oxidation of the heterocycle luciferin. The product possesses one of the highest quantum yields for bioluminescent reactions: one photon is emitted for every oxidized luciferin molecule. The structure of luciferase has recently been solved, allowing for structure-based development of a PCA. As with our GST assay, cells are grown on a nitrocellulose matrix. The addition of the luciferin at the surface of the nitrocellulose permits it to diffuse across the cytoplasmic membranes and trigger the photoluminescent reaction. The detection is done immediately on a photographic film. Luciferase is an ideal candidate for a PCA: the detection assays are rapid, inexpensive, very sensitive, and utilizes non-radioactive substrate that is available commercially. The substrate of luciferase, luciferin, can diffuse across the cytoplasmic membrane (under acidic pH), allowing the detection of luciferase in intact cells. This enzyme is currently utilized as a reporter gene in a variety of expression systems. The expression of this protein has been well characterized in bacterial, mammalian, and in plant cells, suggesting that it would provide a versatile PCA.
- 4) Xanthine-guanine phosphoribosyl transferase (XGPRT) The E. coli enzyme XGPRT converts xanthine to xahthine monophosphate (XMP), a precursor of GMP. Because the mammalian enzyme hypoxanthine-guanine phosphoribosyl transferase HGPRT can only use hypoxanthine and guanine as substrates, the bacterial XGPRT can be used as a dominant selection assay for a PCA for cells grown in the presense of xanthine. Vectors expressing XGPRT confer the ability of mammalian cells to grow in selective medium containing adenine, xanthine, and mycophenolic acid. The function of mycophenolic acid is to inhibit de novo synthesis of GMP by blocking the conversion of IMP into XMP (Chapman A. B., (1983) Molec. & Cellul. Biol. 3, 1421-1429). The only GMP produced then come from the conversion of xanthine into XMP, catalyzed by the bacterial XGPRT. As with aminoglycoside phosphotransferase fragments of XGPRT can be generated based on the known structure (See table 1.) using the design-evolution strategy described above with fragments fused to the GCN4 leucine zippers as a test oligomerization domains. The complementary fusions are cotransfected and the proteins transiently expressed in COS-7 cells, or stability expressed in CHO cells, grown in the selective medium. In the case of CHO cells, colonies are collected and sequentially re-cultured at increasing concentrations of the selective compounds in order to enrich for populations of cells that efficiently express the fusions at high concentrations.
- 5) Adenosine deaminase Adenosine deaminase (ADA) is present in minute quantities in virtually all mammalian cell. Although it is not an essential enzyme for cell growth, ADA can be used in a dominant selection assay. It is possible to establish growth conditions in which the cells require ADA to survive. ADA catalyzes the irreversible conversion of cytotoxic adenine nucleosides to their respective nontoxic inosine analogues. By adding cytotoxic concentrations of adenosine or cytotoxic adenosine analogues such as 9-b-D-xylofuranosyladenine to the cells, ADA is required for cell growth to detoxify the cytotoxic agent. Cells that incorporate the ADA gene can then be selected for amplification in the presence of low concentrations of 2′-deoxycoformycin, a tight-binding transition state analogue inhibitor of ADA. ADA can then be used for a PCA based on cell survival (Kaufman, R. J. et al. (1986) Proc. of the Nat. Acad. Sci. (USA) 83, 3136-3140). As with the other systems described above, fragments of ADA can be generated based on the known structure (See table 1.) using the design-evolution strategy described above with fragments fused to the GCN4 leucine zippers as a test oligomerization domains. The complementary fusions are cotransfected and the proteins transiently expressed in COS-7 cells, or stability expressed in CHO cells, grown in the selective medium containing 2′-deoxycoformycin. In the case of CHO cells, colonies are collected and sequentially re-cultured at increasing concentrations of 2′-deoxycoformycin in order to enrich for populations of cells that efficiently express the fusions at high concentrations
- 6) Bleomycin binding protein (zeocin resistance gene) Zeocin, a member of the bleomycin/phleomycin family of antibiotics, is toxic to bacteria, fungi, plants, and mammalian cells. The expression of the zeocin resistance gene confers resistance to bleomycin/zeocin. The protein confers resistance by binding to and sequestering the drug and thus preventing its association and hydrolysis of DNA. Berdy, J. (1980) In Amino Acid and Peptide Antibiotics, J. Berdy, ed. (Boca Raton, Fla.: CRC Press), pp. 459-497; Mulsant, P., Tiraby, G., Kallerhoff, J., and Perret, J. (1989 Somat. Cell. Mol. Genet. 14, 243-252). Bleomycin binding protein (BBP) could then be used for a PCA based on cell survival. As with the other systems described above, fragments of ADA can be generated based on the known structure (See table 1.) using the design-evolution strategy described above with fragments fused to the GCN4 leucine zippers as a test oligomerization domains. The BBP is a small (8 kD) dimer that binds to drugs via a subunit interface binding site. For this reason, the design would be somewhat different in that first, a single chain form of the dimer would be generated by making a fusion of two BBP genes with a short sequence coding for a simple polypeptide linker introduced between the two subunits. Fragments in this case will be based on a short sequence of one of the subunit modules, while the other fragment will be composed of the remaining sequence of the subunit plus the other subunit. Complementation and selection experiments will be performed as described for the examples above using bleomycin or zeocin as selective drugs.
- 7) Hygromycin-B-phosphotransferase. The antibiotic hygromycin-B is an aminocyclitol that inhibits protein Synthesis by disrupting translocation and promoting misreading. The E. coli enzyme hygromycin-B-phosphotransferase detoxifies the cells by phosphorylating Hygromycin-B. When expressed in mammalian cells, hygromycin-B-phosphotransferase can confer resistance to hygromycin-B (Gritz, L., and Davies, J. (1983) Gene 25, 179-188.). The enzyme is a dominant selectable marker and could be used for a PCA based on cell survival. While the structure of the enzyme is not known it is suspected that this enzyme is homologous to aminoglycoside kinase (Shaw, et al. (1993) Microbiol. Rev. 57, 138-163). It is therefore possible to use the combined design/evolution strategy to produce a PCA with this enzyme and perform dominant selection in mammalian cells with selection at increasing concentrations of hygromycin B.
- 8) L-histidinol NAD+oxydoreductase. The hisD gene of Salmonella typhimurium codes for the L-histidinol NAD+oxydoreductase that converts histidinol to histidine. Mammalian cells grown in media lacking histidine but containing histidinol can be selected for expression of hisD (Hartman, S. C., R. C. Mulligan (1988) Proc. of the Nat. Acad. Sci. (USA) 85, 8047-8051). An additional advantage of using hisD in dominant selection is that histidinol is itself toxic, inhibiting the activity of endogenous histidyl-tRNA synthetase. Histidinol is also inexpensive and readily permeates cells. The structure of histidinol NAD+oxydoreductase is unknown and so development of a PCA based on this enzyme is based entirely on the exonuclease fragment/evolution strategy. The following Table list alternative embodiments using other PCA reporters. Abbreviations in Table: Type: D, dominant selection marker; R, recessive selection marker. Structure: four letter codes=Protein Data Bank (PDB) entries; K, known but not deposited in PDB; U, unknown. mono/oligo: M, monomer; D, dimer; tetra, tetramer.
-
TABLE 1 A list of Other Potential PCA Reporter Candidates A-Assays based on Dominant or Recessive Selection mono/ Enzyme Type Structure Size oligo Selection drugs/Conditions DHFR R/D many 18 kD M methotrexate/trimethoprim Adenosine deaminase D/R 1ADD M Xyl-A or adenosine, alanosine, and 2′- deoxycoformycin Thymidine kinase D/R 1KIN D gangcyclovene, HAT Mutant hypoxanthine- guanine D 1HGM D HAT + thymidine kinase phosphoribosyl transferase Thymidylate synthetase R 1NJE 35 kd M 2 fluorodeoxyuridine Xanthine-guanine D 1NUL mycophenolic acid with phosphoribosyl transferase limiting xanthine Glutamine synthetase R 2LGS Asparagine synthetase R U B-aspartyl hydroxamate or albizin Puromycin N-acetyltransferase D U 23 kD M puromycin Aminoglycoside D K 35 kD M neomycin, G418, gentamycin phosphotransferase Hygromycin B D U M hygromycin B phosphotransferase L-histidinol: NAD+ D U 46 kD M histidinol oxidoreductase Bleomycin binding protein D K 8 kD D bleomycin/zeocin Cytosine methyl-transferase R/D U 5-Azacytidine (5-aza-CR) and 5-aza-2′-deoxycytidine O6-alkylguanine D 1ADN N-methyl-N-nitrosourea alkyltransferase Glycinamide ribonucleotide R 1GRC 23.2 kD D dideazatetrahydrofolate, transformylase minus purine Glycinamide ribonucleotide R U 45.9 kD minus purine synthetase Phosphoribosyl-aminoimidazole R U 36.7 kD minus purine synthetase Formylglycinamide ribotide R U 141.4 kD M L-azaserine, 6-diazo-5-oxo- amidotransferase L-norleucine, minus purine Phosphoribosyl-aminoimidazole R U 39.5 kD D minus purine carboxylase Phosphoribosyl-aminoimidazole R U 57.3 kD minus purine carboxamide formyltransferase Fatty acid Synthase R 272 kD D cerulenin IMP dehydrogenase R 1AK5 55.4 kD Tetra mycophenolic acid Mono/ Enzyme Type Structure Size Oligo Selection drugs/Conditions ii-Viral Plaque Assays Thioredoxin D 1TDF 34.5 kD D Reverse transcriptase D 3HVT Viral protease D B-Cell Death Assays Cysteine protease: papain D 1STF 38.9 kD M inhibited by cystatin Cysteine protease: caspase D 1CP3 17 kD + HeteroD inhibited by DEVD-aldehyde 12 kD (can also by used in a fluorimetric or colorimetric assay, in vitro) Metalloprotease: D 47.1 kD M inhibited by methyl-ethyl carboxypeptidase succinic acid Serine protease: proteinase D 1PTK 30.6 kD M inhibited by serpins K Aspartic protease: pepsin D 1PSN 34.5 kD M inhibited by pepstatin A (can also be used in an fluorimetric assay, in vitro) Lysozyme D many 23.2 kD M inhibited by N- acetylglucosamine trisaccharide RNAse D many 13.3 kD M inhibited by RNAse inhibitor DNAse D 1DNK 61.6 kD M inhibited by actin Phospholipase A2 D 1P2P 13.8 kD M/D many inhibitors: bromophenacyl bromide, hexadecyl- trifluoroethyl-glycero- phosphomethanol, bromoenol lactone, etc. Phospholipase C D 1AH7 28 kD M many inhibitors: neomycin, chelerythrine, U73122, etc. Mono/ Enzyme Structure Size Oligo Selection drugs/Conditions C-Colorimetric/Fluorimetric Assay DT-Diaphorase (NAD(P)H- 1QRD 26 kD D NADPH-diaphorase stain, inhibited [quinone acceptor] by dicumarol, Cibacron blue and oxidoreductase) phenidione Note: can also be used in a cell death assay (+nitrobenzimidazole, fo example). (NAD(P)H-[quinone acceptor] isoform of 21 kD D NRH-diaphorase stain, inhibited by oxidoreductase)-2 1QRD pentahydroxyflavone Thermophilic diaphorase 30 kD M NADH-diaphorase stain (Bacillus stearothermophilus) Glutathione-S-transferase 1GNE 26 kD D production of a yellow product by other the conjugation of glutathione with isoform an aromatic substance, chloro of dinitrobenzene (CDNB) 28 kD Luciferase 1LCI 62 kD M Fluorometric Green-fluorescent protein 1EMA 30 kD M Intrinsic fluorescence Chloramphenicol 1CLA 25 kD Tri Fluorimetric: Bodipy acetyltransferase chloramphenicol Uricase 32 kD Tetra Fluorometric SEAP (secreted form of human 1AJA M CSPD chemiluminescent substrate placental alkaline phosphatase) B-Glucuronidase 1BHG 71 kD Tetra Histochemical, fluorometric or spectrophotometric assays using various substrates such as X-GLUC. D-Heteromeric Enzyme Strategies Tyrosinase 30 kD + Hetero Colorimetric: synthesis of melanin 14 kD M + M - To this point specific examples have only been made of applications of PCA to protein-pair interactions. However, it is possible to apply PCA to multiprotein, protein-RNA, protein-DNA or protein-small molecule interactions. There are two general schemes for achieving, such systems. Multi-subunit PCA: Two proteins need not interact for a PCA signal to be observed; if a partner protein or protein complex binds to two proteins simultaneously, it is possible to detect such a three protein complex. A multisubunit PCA is conceived with the example of herpes simplex virus thymidine kinase (TK), a homodimer of 40 kD. In this conception, the TK structure contains two well defined domains consisting of an alpha/beta (residues 1-223) and an alpha-helical domain (224-374). As a test system, we use the Rop1 dimer, a four helix bundle homodimer. The two fragments of TK are extracted by PCR and subcloned into the transient transfection vector pMT3, the first in tandem to the Adenovirus major late promoter,
tripartite leader 3′ to the first ATG, and the second downstream of a ECMV internal initiation site. Restriction sites previously introduced between the first and the last ATG are subcloned into BamHI/KpnI and PstI/EcoRI cloning sites downstream of the two ATGs. These are used to subclone PCR-generated fragments of the Rop1 subunits into two different vectors. Subsequently Ltk− cells are cotransfected by lipofection with the two plasmids and colonies of surviving cells are serially selected in medium containing increasing concentrations of HAT (hypoxanthine/aminopterin/thymidine). Cells that express complementary fragments of TK fused to the four Rop1 will proliferate under this selective pressure, or otherwise die. Specific examples of use of this concept would be in determining constituents of multiprotein complexes that are formed transiently or constitutively in cells. - The utility of PCA is not limited to detecting protein-protein interactions, but can be adapted to detecting interactions of proteins with DNA, RNA, or small molecules. In this conception, two proteins are fused to PCA complementary fragments, but the two proteins do not interact with each other. The interaction must be triggered by a third entity, which can be any molecule that will simultaneously bind to the two proteins or induce an interaction between the two proteins by causing a conformational change in one or both of the partners. Two examples have been demonstrated in our lab using the mDHFR PCA in E. coli. In the first case a natural product, the immunosuppressant drug rapamycin, is used to induce an interaction between its receptor FKBP12 and a partner protein mTOR (mammalian Target of Rapamycin). We detect this by cotransformation of DHFR fragments fused to FKBP or mTOR into E. coli grown in the presence or absence of trimethoprim (as described above) and rapamycin (0-10 nM). We have demonstrated that support of growth as detected by colony formation is completely dependent on the addition of rapamycin, suggesting that the mDHFR PCA is detecting a rapamycin-induced assembly of a FKBP12-mTOR and subsequent reconstitution of DHFR activity. This is one example of a use of the PCA strategy to test for small molecules that can induce interactions between proteins. General applications could be made to therapeutic development, in the form screening small molecule combinatorial compound libraries for molecules that induce interactions between proteins, that may inhibit the activities of either or both of the proteins, or activate specific cellular processes that are initiated by other events, such as growth factor-mediated receptor dimerization. The discovery of such small molecules could lead to the development of orally available drugs for the treatment of a broad spectrum of human diseases.
- Another example of an induced interaction we have studied with the DHFR PCA is the interaction of the oncogene GTPase p21 ras and its direct downstream target, the serine/threonine kinase raf. This interaction only occurs when the GTPase is in the GTP-bound form, whereas turnover of GTP to GDP leads to release of the complex. As with the FKBP-mTOR complex, we have demonstrated this induced interaction in E. coli. PCA could be used in a general way to study such induced interactions, and to screen for compounds that release or prevent these interactions in pathological states. The ras-raf interaction itself could be a target of therapeutic intervention. Oncogenic forms of ras consist of mutants that are incapable of turning over GTP and therefore remain continuously associated with activated ras. This leads to a constitutive uncontrolled growth signal that results, in part, in oncogenesis. The identification of compounds that inhibit this process, by PCA, would be of value in broad treatment of cancers. Other examples of multimolecular applications of PCA could include identification of novel DNA or RNA binding proteins. In its simplest conception one uses a known DNA or RNA binding motifs, for instance a retinoic acid receptor zinc finger, or a simple RNA binding protein such as IF-1, respectively. One half of the PCA consists of the DNA or RNA protein binding domain fused to one of the PCA fragments (control fragment). The complementary fragment is fused to a cDNA library. A third entity, the gene coding for a sequence containing an element known to bind to the control protein, and then a second putative or known regulatory element is coded for after this sequence. A test system consists of tat/tar elements that control elongation in transcription/translation of HIV genes. An example application would be identification of tat binding elements that have been proposed to exist in eukaryotic genomes and may regulate genes in the same or similar way to that of HIV genes. (SenGupta D. J. et al. (1996) Proc.
Natl. Acad. USA 6, 8496-8501). - Screening combinatorial libraries of compounds for those that inhibit or induce protein-protein/protein-rna/protein-DNA complexes. The PCA strategy can be directly applied to identifying potentially therapeutic molecules contained in combinatorial libraries of organic molecules. It is possible to perform high throughput screening of such libraries to screen for compounds that will inhibit or induce protein-protein interactions or protein-DNA/RNA interactions (as discussed above). In addition it is also possible to screen for compounds that inhibit enzymes whose substrates are other proteins DNA, RNA or carbohydrates, as discussed below. In this application, proteins that interact/protein substrate pairs, or control DNA/RNA binding protein-enzyme pairs are fused to PCA complementary fragments and plasmids harboring these pairs are transformed/transfected into a cell, along with any third DNA or RNA element as the case requires. Transformed/transected cells are grown liquid culture in multiwell plates where each well is inoculated with a single compound from an array of combinatorially synthesized compounds. A readout of a response depends on the effect of a compound. If the compound inhibits a protein interaction, there is a negative response (no PCA signal is the positive response). If the compound induces a protein interaction, the response is a positive PCA signal. Controls for non-specific effects of compounds include: 1) demonstration that the compound does not effect the PCA enzyme itself (test against cells transfected with the wild-type intact enzyme used as the PCA probe) and in the case of a cell survival assay, that the compound is not toxic to the cells that have not been transformed/transfected. As well as providing a high throughput assay for biological activity of compounds, PCA also offers the advantage over in vitro assays that it is a test for cell membrane permeability of active compounds. Specific demonstrated examples of PCA for drug screening in our laboratory include the application of DHFR PCA in E. coli to detecting compounds that inhibit therapeutically relevant targets. These include Bax/Bcl2 fkbp12/tor ras/raf, carboxyl terminal dimerization domain of HIV-1 capsid protein, IkB kinase IKK-1 and IKK-2 dimerization domains (leucine zippers and helix-loop-helix domains). In each case, the two proteins are subcloned 5′ upstream of either F[1,2] or F[3] as described above. Plasmids harboring the complementary fragments are cotransformed into BL21 cells. Colonies from minimal medium plates containing IPTG and trimethoprim are picked, and grown in liquid medium under the same selective conditions and frozen stocks made. For a single screening cycle, a priming overnight culture is grown from frozen stocks in LB medium. A selective minimal medium containing trimethoprim, ampicillin, IPTG is aliquoted at 25 ml into each well of a 384 well plate. Each well is then inoculated with 1 ul of an individual sample from a compound array (ArQule Inc.) to give a final concentration of 10 uM. Each well is then inoculated with 2 ml of overnight culture and plates are incubated in a specially adapted shaker bath at 37 C. At 2 hour intervals, plates are read on an optical absorption spectroscopic plate reader coupled to a PC and spreadsheet software at 600 nm (scattering) for a period of 8 hours. Rates of growth are calculated from individual time readings for each well and compared to a standard curve. A “hit” is defined as a case where an individual compound reduces the rate of growth to less than the 95% confidence interval based on the standard deviation for growth rates observed in all of the wells within the test plate. “Near hits” are defined as those cases where growth rates are within the 95% confidence interval. For each of the hits or near hits, the following controls are then performed: The same experiment is performed with BL21 cells that are transformed with empty vector (and no trimethoprim), with vector harboring the full length mDHFR gene, or with cotransfected cells where protein expression is not induced by IPTG. If in all of these cases the compound has no effect, it can be concluded that it is specifically disrupting the protein-protein interaction being tested. Such validated hits or near hits are then retested to establish a dose-response curve for the individual compound, with concentrations varying from 1 pM up to 1 μM by orders of magnitude of 10. The PCA strategy for compound screening can also be applied in the multiprotein protein-RNA/DNA cases as described above, and can easily be adapted to the DHFR or any other PCA in E. coli or in yeast versions of the same PCAs. Such screening can also be applied to enzymes whose targets are other proteins or nucleic acids for known enzyme/substrate pairs or to novel enzyme substrate pairs identified as described below.
- Proteins involved in viral integration processes are examples of targets that could be tested for inhibitors using the PCA strategies. Examples for the HIV virus include:
- i) inhibition of integrase or the transport of the pre-integration complex: protein Ma or vpr
ii) Inhibition of the cell cycle in G2 by vpr (interaction by cyclin B) causing induction of apoptosis.
iii) Inhibition of the interaction of gp160 (precursor of the membrane proteins) with furine. - i) Vpr: nuclear localizing sequence (target): interaction site of vpr with phosphatasesA.
ii) vif: interaction with vimentin (cytoskeleton associated protein).
ii) Vpu: Degradation of CD4 in the RE mediated by the cytoplasmic tail of Vpu.
iii) nef: Myristoylation signal of Nef. - Other general targets for drug screening could include proteins linked neurodegenerative diseases, such as to alpha-synuclein. This protein has been linked to early onset of Parkinson disease and it is present also implicated in Alzheimer disease. There is also β-amyloid proteins, linked to Alzheimers disease.
- An example of protein-carbohydrate interactions that would be a target for drug screening includes the selecting that are generally implicated in inflammation. These cell surface glycoproteins are directly involved in diapedesis.
- A number of tumor supressor genes whos actions are mediated by protein-protein interactions could be screened for potential anti-cancer compounds. These include PTEN, a tumor supressor directly involved in the formation of harmatomas. It is also involved in inherited breast and thyroid cancer. Other interesting tumor supressor genes include p53, Rb and BARC1.
- The examples described above are used for identifying novel molecular interactions involving molecules that merely bind to each other. However detecting the substrates of enzymes is also fully compatible with the PCA strategy as shown below:
- i) Enzymes that form tight complexes or with protein, substrates or induce efficient PCA fragment assembly or
ii) Mutant enzymes that bind tightly to substrate but do not undergo product release because of mutations residues involved in nucleophilic attack and/or product release (substrate trapping). - Enzymes may form tight complexes with their substrates (Kd ˜1-10 μM). In these cases PCA may be efficient enough to detect such interactions. However, even if this is not true, PCA may work to detect weaker interactions. Generally, if the rate of catalysis and product release is slower than the rate of folding-reassembly of the PCA complementary fragments, effectively irreversible folding and reconstitution of the PCA reporter activity will have occurred. Therefore, even if the enzyme and substrate are no longer interacting, the PCA signal is detected. Therefore, the detection of novel enzyme substrates using PCA may be possible, independent of effective substrate Kd or rate of product release. In cases where product release is much faster than PCA fragment assembly/folding and alternative approach is provided by generating “substrate trapping” mutants of the test enzyme. An example of this approach applied to the protein tyrosine phosphatase PTP1B, where substrate trapping mutants have been generated by mutating the nucleophilic aspartate 181 to alanine rendering the enzyme catalyticly dead, but capable of forming tight complexes with a known substrate, the EGF receptor and other unknown proteins (Flint, A. J. et al. (1996) Proc. Natl. Acad. USA 941680-1685). An application of using PCA to screen for interacting partners of PTP1B is given as follows. We use the aminoglycoside kinase (AK)-based PCA in transiently transfected COS or 293 cells. The substrate trap mutant catalytic domain of PTP1B is fused to N-terminal complementary fragment of AK, while a C-terminal fusion of the other AK fragment is made to a cDNA library. Cells are co-transfected with complementary AK pairs and grown in selective concentrations of G418. After 72 hours, colonies of surviving cells are picked and in situ PCR is performed using primers designed to anneal to 3′ and 5′ flanking regions of the cDNA coding region. PCR amplified products are then 5′ sequenced to identify the gene.
- Enzyme inhibitors Screening combinatorial libraries of compounds for those that inhibit enzyme-PROTEIN substrate complexes either with:
- i) Enzymes that form tight complexes with protein substrates or
ii) Mutant enzymes that bind tightly to substrate but do not undergo product release because of the mutation. - The PCA strategy can be used to generate peptides or proteins with novel binding properties that may have therapeutic value, as is done today with phage display technology. It is also possible to develop enzymes with novel substrate or physical properties for industrial enzyme development. Two detailed examples of the application of the PCA strategy to these ends are given below, with additional applications listed below.
- 1) Selection of high-affinity, heterodimerizing leucine zipper sequences (J. Pelletier, K. Arndt, A. Plueckthun and S. Michnick, manuscript in preparation). The mDHFR PCA, described above, was used in a scheme for the selection of efficiently heterodimerizing, designed leucine zippers. It has been proposed that the formation of salt bridges between positively and negatively charged residues at complementing “e” and “g” positions is important in stabilizing leucine zipper formation, though this view has been contested. In order to help define the importance of salt-bridge formation at the e and g positions, two leucine zipper libraries were built. Both are based on the GCN4 leucine zipper sequence, but contain sequence information specific to either Jun or Fos zippers in order to create heterodimerizing pairs. As well, the e-1 to e-4 and g-1 to g-4 positions in each library were randomized to code for positively or negatively charged residues, or neutral polar residues. These libraries were amplified by PCR and subcloned into the Z-F[1,2] or Z-F[3] constructs (described above) from which the GCN4 zipper sequences had been removed. The bacterial mDHFR PCA selection was performed on selective solid media, as described earlier. Colonies were picked and sequenced; sequence analysis reveals that the distribution of charged or neutral residues at e-g pairs is not random, but is biased toward pairing of opposite charges, or pairing of a charged with a neutral residue, rather than same-charge pairing (see
FIG. 7 ). We reasoned that better zipper pairing should lead to an increase in efficiency of DHFR-fragment complementation, resulting in faster bacterial doubling times (see Table 1 in the mDHFR PCA description), and undertook a selection/enrichment of the novel zippers relative to GCN4, as follows. The designed zipper libraries, expressed as N-terminal fusions to the DHFR F[1,2] or F[3:I114A], were cotransformed, clones were picked, propagated and mixed in selective liquid culture, and the mix was added in a 1:1 000 000 ratio to clone Z-F[1,2]+Z-F[3:I114A] (original GCN4 leucine zippers). The mixture was propagated in selective liquid culture over multiple passages. Restriction analysis shows that within 4 passages, the population of GCN4-expressing bacteria is diminishing relative to the novel zipper sequences (data not shown), indicating that some of the designed zipper-containing clones are propagated at a higher rate than those containing GCN4. Bacteria from later passages were plated on selective medium, and individual clones sequenced to reveal the identity of the most successful designed zipper pairs (data not shown). - 2) Application of PCA to enzyme function and design PCA Development: Adenosine deaminase (ADA) meets all of the criteria for a PCA listed above. ADA is a small (˜40 kD), and easily purified monomeric zinc metallo-enzyme and the structure of murine ADA has been resolved. Several in vitro ADA activity assays have been developed, involving UV spectrophotometry and stopped-flow fluorimetry. E. coli ADA catalyzes the irreversible conversion of cytotoxic adenine nucleosides to non-toxic inosines.
- Eukaryotic or prokaryotic cells propagated in the presence of cytotoxic concentrations of adenosine or adenosine analogs require ADA to detoxify these compounds. This is the basis of a dominant-selection strategy used to select for cells expressing a specific gene in mammalian cells. The ADA gene has also been expressed in SF3834 E. coli cells which lack a gene coding for endogenous ADA. When the gene coding for ADA is introduced into ADA-bacterial DNA, those cells that express ADA are able to survive high concentrations of added adenosine; those that do not, die. This forms the basis of an in vivo ADA activity assay.
- We chose ADA, principally because it can be used as a dominant selective marker in mammalian and bacterial cells where the gene has been knocked out. The reason we choose dominant selective genes is because in screening for novel protein-protein interactions, particularly testing for interactions of a known protein against a library of millions of independent clones, selection serves to filter for cells that may show a positive response for reasons having nothing to do with a specific protein-protein interaction. We will use three test systems of interacting proteins including leucine zipper-forming sequences, the proteins raf and p21 and the induced oligomerization system, FK506 binding protein (FKBP) and mTOR that interact through the macrocyclic immuno-suppressant compound rapamycin. For all of these systems, we will construct E. coli and mammalian transient transfection plasmids and subclone the test proteins as fusions to ADA complementary fragments. The primary assay will be survival of SF3834 E. coli cells that have been transformed with the complementary ADA fragments fused to the test oligomerization proteins in the presense of toxic concentrations of adensosine. We will then purify fusion proteins from colonies of and perform in vitro assays of ADA activity as described below. The utility of the ADA PCA as a method to identify novel proteins that interact with a test bait will be performed in mammalian COS-7 and HEK-293T cells transiently transfected with FKBP fused to one of the ADA fragments and the other fragment fused to a cDNA library from normal human spleen containing 106 independent clones. As with the E. coli assay, cells that survive in a medium containing toxic concentrations of ADA is collected and isolated plasmids will be tested to identify the gene for the interacting protein by PCR amplification and chain propagation-termination techniques.
- Structural motifs required for protein function: Determination of the structural elements required for the enzymatic function of ADA are investigated through alteration of the structures of the enzyme fragments. At first, ADA is cut into two separate domains—one responsible for substrate binding (residues 1-210) and one responsible for catalysis (residues 211-352). These separate pieces will be attached to known assembly domains, such as leucine zippers (see example 1 above). Reassembly will restore activity which will be assessed through detailed in vitro kinetic analysis of the binding and catalytic properties of the re-assembled enzyme, using UV spectrophotometry and stopped-flow fluorimetry to observe the enzymatic reactions. This system will provide another handle on the manipulation of enzyme activity that will afford a powerful tool for enzymatic mechanism study. For example, the difference in the kinetic behaviour of the reassembled enzyme on mixing with the substrate, compared to enzyme reassembled in presence of substrate (where substrate may already be bound by binding domain) will allow sophisticated level of study of importance of binding energy to catalysis. Subsequent point mutations to the functional or assembly domains of the proteins will then allow a very subtle perturbation and detailed quantification of the relationship of binding energy to catalysis. This precise control over the structure and assembly of separate functional domains of the enzyme will permit very sophisticated enzymatic structure function studies, the definition of structural motifs and an understanding of their role in catalysis.
- Novel protein catalyst design: The detailed knowledge of the enzyme mechanism gained through determination of the structural requirements for catalysis will then be exploited through the combination of these functional “building blocks” with the functional motifs responsible for substrate binding and catalysis in other enzymes, allowing the generation of novel protein catalysts. For example, the catalytic motif from ADA is modified to a cytidine-binding motif, creating a novel enzyme with potentially useful catalytic properties. The activity of these novel enzymes can easily be assessed through in vivo assays similar to that of the PCA system, or through in vitro activity assays. Furthermore, the detailed mechanistic investigation of the resulting enzymes possible with this system will permit the rational design of each subsequent generation of catalysts.
- It is a logical extension of the descriptions of PCA applications above to the utility of these techniques in whole model organisms such as drosophila, nematodes, zebra fish and puffer fish, as examples. The sole differences with other listed examples is that vectors used would need to be different (for example retroviral vectors) and that any substrates needed by the PCA would need to be bioavailable, or detection would need to be performed in situ.
- The recent development and commercial availability of fluorescence imaging systems for whole animals (Chishima et al., Cancer Research 57: 2042-2047, 1997) makes it possible to apply this approach in mice, using in situ detection of the fluorescence signal generated by the PCA. For this purpose, PCAs were generated using mammalian expression vectors and cell lines stably expressing the PCAs were created and then implanted into mice. We used a fluorescent protein to create the PCAs, so that no other substrates or cofactors would be required to generate a fluorescent signal.
- First, GFP fragments were generated by PCR from a mammalian codon-optimized version of GFP (pCMS-EGFP; Clontech). GFP[1] corresponded to
amino acids 1 to 158 and GFP[2] to amino acids 159 to 239 of GFP. Second, fragments encoding a variant of GFP (YFP PCA) were created by introducing the EYFP-specific mutations S65G, S72A intofragment 1 of GFP and the mutation T203Y intofragment 2 of GFP by PCR, resulting in fragments YFP[1] and YFP[2], respectively. The fragments YFP[1], and YFP[2] were subcloned into a mammalian expression vector (pcDNA3.1Z, Invitrogen), which had previously been modified to incorporate the replication origin (oriP) of the Epstein Barr virus (EBV). The oriP allows episomal replication of these modified vectors in cell lines expressing the EBNA1 gene, such as HEK293E cells (293-EBNA, Invitrogen). Additionally, these vectors still retain the SV40 origin, allowing for episomal expression in cell lines expressing the SV40 large T antigen (e.g. HEK293T, Jurkat or COS) as well. - Gene-fragment fusions were created as follows. The YFP[1] and YFP[2] fragments were amplified by PCR to incorporate restriction sites and a linker sequence, described below, in configurations that would allow fusion of a gene of interest to either the 5′- or 3′-end of each reporter fragment. The reporter-linker fragment cassettes were subcloned into a mammalian expression vector (pcDNA3.1Z, Invitrogen) that had been modified to incorporate the replication origin (oriP) of the Epstein Barr virus (EBV). The oriP allows episomal replication of these modified vectors in cell lines expressing the EBNA1 gene, such as HEK293E cells (293-EBNA, Invitrogen). Additionally, these vectors still retain the SV40 origin, allowing for episomal expression in cell lines expressing the SV40 large T antigen (e.g. HEK293T, Tag-Jurkat or COS). The integrity of the mutated reporter fragments and the new replication origin were confirmed by sequencing.
- PCAs were then created for pairs of full-length mammalian proteins that are known to interact in vivo (MEK/ERK and BAD/14-3-3sigma). The open reading frames of full-length rat mitogen-activated protein kinase 1 (MEK1), extracellular signal-regulated
kinase 1 ERK1 (MAPK1), BAD and 14-3-3sigma were amplified by PCR from a sequence-verified full-length cDNA and then fused in-frame to complementary reporter fragments. Resulting PCR products were column purified (Centricon), digested with appropriate restriction enzymes to allow directional cloning, and fused in-frame to either the 5′ or 3′-end of YFP[1] or YFP[2] through a linker encoding a flexible 10 amino acid peptide (Gly.Gly.Gly.Gly.Ser)2. ERKERK1 was ligated to the 5′ end of YFP[1] while MEK1 was appended to YFP[2] in N-terminal fusions. BAD was fused 3′ of YFP[2] fragment and 14-3-3s was fused 5′ of the YFP[1] fragment. The fusion genes were subcloned into pcDNA3.1 expression vectors (Invitrogen) with Zeocin selectable marker for YFP[1]-fusions and hygromycin marker for YFP[2]fusions. Recombinants in the host strains DH5-alpha (Invitrogen, Carlsbad, Calif.) or XL1 Blue MR (Stratagene, La Jolla, Calif.) were screened by colony PCR, and clones containing inserts of the correct size were subjected to end sequencing to confirm the presence of the gene of interest and in-frame fusion to the appropriate reporter fragment. A subset of fusion constructs were selected for full-insert sequencing by primer walking. DNAs were isolated using Qiagen MaxiPrep kits (Qiagen, Chatsworth, Calif.). PCR was used to assess the integrity of each fusion construct, by combining the appropriate gene-specific primer with a reporter-specific primer to confirm that the correct gene-fusion was present and of the correct size with no internal deletions. - HEK293T cells were grown in MEM alpha medium (Invitrogen) supplemented with 10% FBS (Gemini Bio-Products), 1% penicillin, and 1% streptomycin and maintained in a 37° C. incubator at 5% CO2. HEK293T cells were co-transfected with complementary PCA fragment pairs fused to full-length genes encoding proteins known to form interactions. Stable cell lines expressing the PCAs of interest were isolated following double antibiotic selection with 50 micrograms/ml Hygromycin B and 500 micrograms/ml Zeocin. The resulting fluorescence signals, generated by protein-fragment complementation, are shown in
FIG. 10 . The YFP[2]-BAD interaction with 14-3-3s-YFP[1] resulted in reconstitution of YFP fluorescence with the expected cytosolic localization, as did the interaction of ERK1-YFP[1] with MEK1-YFP[2]. For all cell lines, the fluorescence signals were stable over at least 25 passages. - To demonstrate visualization of the PCA signal in live animals, four BALB/c nu/nu mice were injected subcutaneously, ventrally with a single dose of the human cells, as follows: (a) parental 293T cells; (b) cells stably expressing the Bad/14-3-3sigma PCA; and (c) cells stably expressing the MEK/ERK PCA. Each animal received 3 injections (one for each cell type) with 5×10exp6 cells per site. Fluorescence due to the PCA was immediately imaged with a whole-animal imaging system (AntiCancer Inc., San Diego, Calif.). Both of the PCA-engineered cells generated patches of fluorescence in live animals following injection (example shown in
FIG. 11 ). All four animals showed clearly visible fluorescence at both the Bad/14-3-3 and MEK/ERK sites, and none at the parental cell site. - The results demonstrate that the PCA strategy can be used to detect molecular interactions in whole organisms. Any of the reporters described in Table 1, that allow detection in living organisms, can be used for this aspect of the invention. For example, a variety of fluorescent proteins and luminescence reporters, including luciferase, can be used in conjunction with this invention.
- In addition to the use of animal xenografts as described herein, the PCA strategy can be applied to transgenic organisms. In addition the strategy can be used in conjunction with gene therapy, for example, to follow the results of gene introduction or of gene silencing. This strategy will also be useful in monitoring drug effects in live animals, in particular, in monitoring of the pharmacokinetics and pharmacodynamics of drug action or of gene therapy.
- Another important embodiment of the invention is to provide a means and method for gene therapy of mammalian disease. Of particular interest is the use of PCA therapeutic for treatment of cancer. In one embodiment of said PCA gene therapy, a PCA is developed employing fragments (modular protein units) derived from a protein toxin for example: Pseudomonas exotoxin, Diptheria toxin and the plant toxin gelonin, or other like molecules. For therapy of breast cancer for example, first a mammalian, retroviral, adenoviral, or eukaryotic artificial chromosomal (EAC's) genetic construct is prepared that introduces one fragment of the selected toxin under the control of the promoter for expression of the erbB2 oncogene. Its is well known that the erbB2 oncogene is overexpressed in breast cancer and adenocarcinoma cells (D. J. Slamon et. al., Science, 1989, 244, 707). The HER2/neu (c-erbB-2) proto-oncogene encodes a
sub-class 1 185-kDa transmembrane protein tyrosine kinase growth factor receptor, p185HER2. Also, the human erbB2 oncogene is located on chromosome 17, region q21 and comprises 4,480 base pairs and p185HER2 serves as a receptor for a 30-kDa glycoprotein growth factor secreted by human breast cancer cell lines (R. Lupu et. Al., Science, 1990, 249, 1152). - The transgene is introduced ‘in vivo’ or ‘ex-vivo’ into target cells employing methods known by those skilled in the art e.g. homologous recombination to insert transgene into locus of interest via retroviral, adenoviral or EAC's. A second genetic construct comprising a fusion gene containing a target DNA that encodes an interacting protein that interacts with erbB2 oncogene discovered by the PCA process described in this invention and the “second” fragment of the toxin molecule. This construct is delivered to the patient by methods known in the art for example as shown in U.S. Pat. Nos. 5,399,346 and 5,585,237 whose entire contents are incorporated by reference herein. Transgene expression of the erbB2 oncogene-toxin fragment described will now be under the control of the constitutive oncogene promoter. Proliferating tumor cells will thus produce one piece of the toxin attached as a fusion to the erbB2 oncogene. In the presence of the second genetic construct expressing the PCA discovered interacting erbB2 oncogene “interacting protein-toxin fragment” construct then: erbB2 oncogene-toxin fragments: interacting protein-toxin fragment B will be created and induce death of target tumor cells through creation of an active toxin through Protein Fragment Complementation and thus provide an efficacious and efficient therapy of said disease.
- This can be extended to other diseases and other toxins employing techniques described and embodied in this invention.
- Any of the PCA strategies described above could be adapted to in vitro detection. Unlike the in vivo PCAs however, detection would be performed with purified PCA fragment-fusion proteins. Such uses of PCA have the potential for use in diagnostic kits. For example the test DHFR assay described above where the interacting domains are FKBP12 and TOR could be used as a diagnostic test for rapamycin concentrations for use in monitoring dosage in patients treated with this drug.
- As shown above, the instant invention provides:
- 1) Allow for the detection of protein-protein interactions in vivo or in vitro.
2) Allow for the detection of protein-protein interactions in appropriate contexts, such as within a specific organism, cell type, cellular compartment, or organelle.
3) Allow for the detection of induced versus constitutive protein-protein interactions (such as by a cell growth or inhibitory factor).
4) To be able to distinguish specific-versus non-specific protein-protein interactions by controlling the sensitivity of the assay.
5) Allow for the detection of the kinetics of protein assembly in cells.
6) Allow for screening of cDNA libraries for protein-protein interactions. - Further aspects of the invention can be demonstrated by identifying novel interactions with the enzyme p70S6k, to determine its' regulation and how separate signaling cascades converge on this enzyme.
- The PCA method is particularly useful for detection of the kinetics of protein assembly in cells. The kinetics of protein assembly can be determined using fluorescent protein systems.
- In a further embodiment of the invention, PCA can be used for drug screening. The techniques of PCA are used to screen for drugs that block specific biochemical pathways in cells allowing for a carefully targeted and controlled method for identifying products that have useful pharmacological properties.
- Although the present invention has been described herein above by way of preferred embodiments thereof, it can be modified, without departing from the spirit and nature of the subject invention as defined in the appended claims.
-
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Abbreviations: PCA, Protein-fragment Complementation Assay; mDHFR, murine dihydrofolate reductase; hDHFR, human dihydrofolate reductase; Z-F[1,2], GCN4 leucine zipper-mDHFR fragment[1,2]; USPS, ubiquitin-based split-protein sensor; IPTG, isopropyl-b-D-thiogalactopyranoside; PMSF, phenylmethylsulfonyl fluoride; SDS-PAGE, SDS polyacrylamide gel electrophoresis.
Claims (7)
1-8. (canceled)
9. Protein fragment complementation assays to characterize protein function and drug function in whole animals wherein said animals have been injected with cells transfected with nucleic acids coding for fusion products of protein reporter fragments and proteins capable of interacting, said assays comprising a reassembly of separate fragments of said protein reporter, wherein reassembly of said protein reporter fragments is operated by the interaction of molecular domains fused to each reporter fragment of said proteins capable of interacting, and wherein reassembly of the protein reporter fragments is independent of other molecular processes with the proviso that said protein reporter molecule is not ubiquitin and said assay is not a two hybrid assay.
10. A method for detecting biomolecular interactions in whole animals having neoplastic diseases said method comprising: (a) selecting an appropriate nucleic acid coding for a protein reporter molecule; (b) effecting fragmentation of said nucleic acid coding for said protein reporter molecule such that said fragmentation results in reversible loss of reporter function; (c) providing a nucleic acid molecule coding for a protein fusion product, which molecule comprises sequences coding for a product selected from the group consisting of: (i) a first protein fusion product comprising: (1) fragments of a first protein molecule whose fragments can exhibit a detectable activity when associated and (2) a second molecule fused to the fragment of the first protein molecule: (ii) a second fusion product comprising (1) a second fragment of said first protein molecule and (2) a second or third molecule and (iii) both (i) and (ii); (d) transfecting suitable cells with nucleic acids coding for the fusion products of (c); (e) injecting said transfected cells into a whole animal; and (f) detecting the reassociation of said protein reporter fragments through interactions of the molecules that are fused to said fragments with the proviso that said protein reporter molecule is not ubiquitin and said method is not a two hybrid method.
11. The method of claim 10 wherein said reporter molecule is a fluorescent or luminescent protein.
12. The method of claim 11 wherein said fluorescent protein is green fluorescent protein or a yellow fluorescent protein.
13. A method for detecting protein-protein interactions in cancer cells, said method comprising: (a) synthesizing probe protein fragments from a protein which enables fluorescent or luminescent detection by dissecting the gene coding for the fluorescent or luminescent protein into at least two fragments; (2) constructing fusion proteins consisting of the probe protein fragments linked to protein domains that are to be tested for interactions; (3) co-expressing the fusion proteins; and (d) detecting reconstitution of the fluorescence or luminescence signal.
14. A method according to claim 13 wherein a signal is generated in a living organism.
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US7062219B2 (en) * | 1997-01-31 | 2006-06-13 | Odyssey Thera Inc. | Protein fragment complementation assays for high-throughput and high-content screening |
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US6828099B2 (en) * | 1998-02-02 | 2004-12-07 | Odyssey Thera Inc. | Protein fragment complementation assay (PCA) for the detection of protein-protein, protein-small molecule and protein nucleic acid interactions based on the E. coli TEM-1 β-Lactamase |
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