US20050215637A1 - Tuberculosis treatment - Google Patents

Tuberculosis treatment Download PDF

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US20050215637A1
US20050215637A1 US10/509,502 US50950204A US2005215637A1 US 20050215637 A1 US20050215637 A1 US 20050215637A1 US 50950204 A US50950204 A US 50950204A US 2005215637 A1 US2005215637 A1 US 2005215637A1
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compound
formula
mutilin
acetyl
meaning
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US10/509,502
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Gerd Ascher
Friedrich Stauffer
Heinz Berner
Rosemarie Mang
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Individual
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Priority claimed from GB0217149A external-priority patent/GB0217149D0/en
Priority claimed from GB0217305A external-priority patent/GB0217305D0/en
Application filed by Individual filed Critical Individual
Publication of US20050215637A1 publication Critical patent/US20050215637A1/en
Priority to US11/818,094 priority Critical patent/US8088823B2/en
Priority to US13/317,666 priority patent/US20120046276A1/en
Abandoned legal-status Critical Current

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    • C07D233/00—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings
    • C07D233/54—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having two double bonds between ring members or between ring members and non-ring members
    • C07D233/64—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having two double bonds between ring members or between ring members and non-ring members with substituted hydrocarbon radicals attached to ring carbon atoms, e.g. histidine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/13—Amines
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    • A61K31/13—Amines
    • A61K31/15—Oximes (>C=N—O—); Hydrazines (>N—N<); Hydrazones (>N—N=) ; Imines (C—N=C)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/13—Amines
    • A61K31/155—Amidines (), e.g. guanidine (H2N—C(=NH)—NH2), isourea (N=C(OH)—NH2), isothiourea (—N=C(SH)—NH2)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19—Carboxylic acids, e.g. valproic acid
    • A61K31/195—Carboxylic acids, e.g. valproic acid having an amino group
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    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
    • A61K31/445—Non condensed piperidines, e.g. piperocaine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A61P31/06—Antibacterial agents for tuberculosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P33/00—Antiparasitic agents
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    • C07C323/00—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups
    • C07C323/50—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and carboxyl groups bound to the same carbon skeleton
    • C07C323/51—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and carboxyl groups bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton
    • C07C323/52—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and carboxyl groups bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton the carbon skeleton being acyclic and saturated
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    • C07D207/04—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members
    • C07D207/10—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
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    • C07D211/00—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings
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    • C07D211/06—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members
    • C07D211/08—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms
    • C07D211/18—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms with substituted hydrocarbon radicals attached to ring carbon atoms
    • C07D211/20—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms with substituted hydrocarbon radicals attached to ring carbon atoms with hydrocarbon radicals, substituted by singly bound oxygen or sulphur atoms
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    • C07D211/00—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings
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    • C07D211/06—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members
    • C07D211/08—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms
    • C07D211/18—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms with substituted hydrocarbon radicals attached to ring carbon atoms
    • C07D211/20—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms with substituted hydrocarbon radicals attached to ring carbon atoms with hydrocarbon radicals, substituted by singly bound oxygen or sulphur atoms
    • C07D211/24—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms with substituted hydrocarbon radicals attached to ring carbon atoms with hydrocarbon radicals, substituted by singly bound oxygen or sulphur atoms by sulfur atoms to which a second hetero atom is attached
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    • C07D211/06—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members
    • C07D211/36—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
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    • C07D211/00—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings
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    • C07D211/06—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members
    • C07D211/36—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
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    • C07D211/06—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members
    • C07D211/36—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
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    • C07D211/06—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members
    • C07D211/36—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D211/60—Carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
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    • C07D211/68—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having one double bond between ring members or between a ring member and a non-ring member
    • C07D211/72—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having one double bond between ring members or between a ring member and a non-ring member with hetero atoms or with carbon atoms having three bonds to hetero atoms, with at the most one bond to halogen, directly attached to ring carbon atoms
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    • C07D223/06—Heterocyclic compounds containing seven-membered rings having one nitrogen atom as the only ring hetero atom not condensed with other rings with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
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    • C07D295/16—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms acylated on ring nitrogen atoms
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    • C07D493/04—Ortho-condensed systems

Definitions

  • the present invention relates to tuberculosis treatment, such as treatment of diseases mediated by Mycobacterium , e.g. Mycobacterium tuberculosis , with pleuromutilins.
  • Tuberculosis is a chronic infectious disease mediated by infection with Mycobacterium tuberculosis .
  • Tuberculosis is a major disease in developing countries, as well as an increasing problem in developed areas of the world. Although the infection may be asymptomatic for a considerable period of time, the disease is most commonly manifested as an acute inflammation of the lungs, resulting in fever and a nonproductive cough. If untreated, serious complications and death typically result.
  • Tuberculosis may be generally controlled by antibiotic therapy, such as by treatment with Isoniazid, see e.g. The Merck Index, 12th edition, item 5203; Rifampin (Rifampicin®), see e.g. The Merck Index, 12th edition, item 8382, Streptomycin, see e.g. The Merck Index, 12th edition, item 8983; but a major problem is the development of strain drug resistance against such antibiotics.
  • the present invention provides the use of a pleuromutilin in the preparation of a medicament for the treatment of diseaeses mediated by Mycobacterium.
  • the present invention provides a method of treating diseases mediated by Mycobacterium , comprising administering to a subject in need of such treatment an effective, e.g. an anti-mycobacterium effective; amount of a pleuromutilin.
  • Mycobacterium includes M. tuberculosis . Diseaeses mediated by Mycobacterium include mycobacterium infections.
  • a pleuromutilin for treatment includes one or more pleuromutilins, e.g. a combination of different pleuromutilins. Treatment includes treatment and prophylaxis.
  • a pleuromutilin for use according to the present invention or for treating diseases according to the present invention is designated hereinafter as “a pleuromutilin(s) of (according to) the present invention”.
  • a pleuromutilin of the present invention includes a pleuromutilin in the form of a free base, and, where existing, in the form of a salt, in the form of a solvate and in the form of a salt and a solvate, e.g. and in the form of a complex, such as a cyclodextrin complex.
  • a pleuromutilin of the present invention may exist in the form of isomers and mixtures thereof, e.g. including diastereoisomers and mixtures thereof. Isomeric mixtures may be separated as appropriate, e.g. according to a method as conventional, to obtain pure isomers.
  • the present invention includes a pleuromutilin according to the present invention in any isomeric form and in any isomeric mixture, such as described in patent literature cited below, which patent literature is introduced herein by reference with respect to isomeric forms of pleuromutlins.
  • a pleuromutilin according to the present invention in any isomeric form and in any isomeric mixture, such as described in patent literature cited below, which patent literature is introduced herein by reference with respect to isomeric forms of pleuromutlins.
  • the cofiguration in the mutilin ring is the same as in a naturally produced mutilin.
  • Pleuromutilin a compound of formula is a naturally occurring antibiotic, e.g. produced by the basidomycetes Pleurotus mutilus and P. pasckerianus , see e.g. The Merck Index, 12th edition, item 7694.
  • a pleuromutilin of the present invention includes a pleuromutilin having the basic structural elements as set out in formula wherein R is vinyl or ethyl and the dotted line is a bond or is no bond.
  • the dotted line between positions 19 an 20 is a bond or is no bond.
  • a hydrogen atom in positions 4, 7 and/or 8 of the ring system may be replaced by deuterium, and if the dotted line between positions 1 and 2 is no bond (single bond between positions 1 and 2) the ring system may be further substituted in positions 1 and/or 2, e.g. by halogen, deuterium or hydroxy.
  • the group —O— in position 14 is further substituted, preferably by a substituted carbonyl group.
  • pleuromutilins examples include e.g.
  • the present invention provides a pleuromutilin, which is selected from the group consisting of compounds of formulae
  • R EX is as set out in TABLE 1.
  • the compounds are of formula I EX , with the exception of Example 12 in which the compound is of formula I′ EX .
  • Example 2 14-O-[(2-(R*)-((R)-Histidinyl)-amino-cyclohexan-1-(S*)-yl)-sul- fanyl acetyl)]-mutilin in the form of a hydrochloride (d 6 -DMSO): Diastereoisomers: 8.4, 9.0(2xm, 2H, NH), 7.5,
  • the present invention provides a compound of formula wherein
  • the present invention provides 14-O-(oximino-(C 3-8 )cycloalkyl-sulfanylmethylcarbonyl)-pleuromutilins and 14-O-(hydrazono-(C 3-8 )cycloalkyl-sulfanylmethylcarbonyl)-pleuromutlins, such as a compound of formula wherein R 1B has the meaning of R 1A as defined above,
  • Example 33 14-O- ⁇ [(3-(2-Diethylamino-ethoxyimino)-cycl
  • Example 35 14-O- ⁇ [(3-(2-Piperidine-1-yl-ethoxyimino)-cyclohept-(R/S)-yl)-sul- fanyl]-acetyl ⁇ -mutilin in the form of a hydrochloride(E/Z mixture)
  • the present invention provides 14-O-(hydroxy- or oxo)-(heterocyclyl-sulfanylmethylcarbonyl)-pleuromutlins, wherein heterocyclyl is an aliphatic ring of 4 to 8 ring members, preferably 5 to 7, comprising one nitrogen as the heteroarom, e.g. a compound of formula wherein
  • R EX is as set out in TABLE 5.
  • the compounds of TABLE 5 are compounds of formula I EX , with the exception of the compound of Example 41 which is a compound of formula I′′ EX .
  • Novel compounds provided by the present invention including compounds, the formulae of which are as set out in TABLE 1 to TABLE 5, and compounds of formulae I A , I B , I C , I D , I EX , I′ EX and I′′ EX are herein designated as “novel compound(s) of (according to) the present invention”.
  • the residue of an amino acid whenever defined in any one of the novel compounds of the present invention includes that part of an amino acid, e.g. including natural and synthetic amino acids, e.g. valine and other amino acids as defined herein, most preferably valine; which remains if the hydroxy group from the carboxylic acid group is splitt off, e.g. In case of valine [HO—CO—CH(NH 2 )—CH(CH 3 ) 2 ] the residue —CO—CH(NH 2 )—CH(CH 3 ) 2 .
  • each single defined substitutent may be a preferred substituent, e.g. independently of each other substitutent defined.
  • a novel compound of the present invention includes a compound in any form, e.g. In free form, in the form of a salt, in the form of a solvate and in the form of a salt and a solvate.
  • the present invention provides a novel compound of the present invention in the form of a salt.
  • a salt of a novel compound of the present invention includes a pharmaceutically acceptable salt, e.g. including a metal salt or an acid addition salt.
  • Metal salts include for example alkali or earth alkali salts;
  • acid addition salts include salts of a compound of formula I with an acid, e.g. hydrogen fumaric acid, fumaric acid, naphthalin-1,5-sulphonic acid, phosphoric acid, tartaric acid, citric acid, hydrochloric acid, deuterochloric acid; preferably hydrochloric acid.
  • a novel compound of the present invention in free form may be converted into a corresponding compound in the form of a salt; and vice versa.
  • a novel compound of the present invention in free form or in the form of a salt and in the form of a solvate may be converted into a corresponding compound in free form or in the form of a salt in unsolvated form; and vice versa.
  • a novel compound of the present invention may exist in the form of isomers and mixtures thereof; e.g. optical isomers, diastereoisomers, cis-, trans-conformers.
  • a compound of the present invention may e.g. contain asymmetric carbon atoms and may thus exist in the form of diastereoisomeres and mixtures thereof, e.g. racemates.
  • a novel compound of the present invention may comprise the residue of an amino acid. In such amino acid residue the carbon atom to which the amino group is attached may be an asymmetric carbon atom and the amino group attached may thus be in the R- or S-configuration.
  • a novel compound of the present invention may comprise a cycloalkyl, e.g.
  • the carbon atom of a cycloalkyl group to which the sulfanylgroup is attached may be asymmetric, e.g. if said cycloalkyl Is further substituted, and substitutents attached to said cycloalkyl group may be in the R- or in the S-configuration.
  • a novel compound of the present invention also may comprise an oxime group.
  • the hydroxgroup attached to the imino group may be in syn- or in anti-configuration. Isomeric mixtures may be separated as appropriate, e.g.
  • the present invention includes a novel compound of the present invention in any isomeric form and in any isomeric mixture.
  • the present invention also includes tautomers of a novel compound of the present invention where such tautomers can exist.
  • Any compound described herein, e.g. a novel compound of the present invention, may be prepared as appropriate, e.g. according to a method as conventional, e.g. analogously, e.g. or as specified herein.
  • the present invention provides a process for the production of a compound of formula wherein
  • a process provided by the present invention may e.g. be carried out analogously to processes as set out In any of the patent references cited herein, e.g. analogously to a process for the production of compounds as described in patent literature cited herein, such as in WO0109095, WO0204414 and WO0222580, or as described herein.
  • a pleuromutilin of the present invention includes the novel compounds of the present invention of formula I P .
  • a pleuromutilin of the present invention includes one or more, preferably one, pleuromutilins of the present invention, e.g. one pleuromutilin or a combination of different pleuromutlins of the present invention.
  • novel compounds of the present invention exhibit pharmacological activity similar to pleuromutilins in similar indications as described in WO0109095, WO0204414 and WO0222580, e.g. in test systems similar as described in WO0109095, WO0204414 and WO0222580, and additionally in test systems described herein.
  • Compounds of formula I P are therefore useful as pharmaceuticals.
  • the present invention provides a compound of formula I P for use as a pharmaceutical, preferably as an antimicrobial, such as an antibiotic, e.g. and as an anti-anaerobic, including the use as a pharmaceutical in the treatment of diseases mediated by Mycobacterium , such as Mycobacterium tuberculosis.
  • an antimicrobial such as an antibiotic
  • Mycobacterium such as Mycobacterium tuberculosis
  • the present invention provides a compound of formula I P for use in the preparation of a medicament for the treatment of microbial diseases, for example of diseases mediated by bacteria, e.g. selected from Staphylococci, Streptococci, Enterococci, Mycobacterium , e.g. Mycobacterium tuberculosis ; e.g. and of diseases mediated by Mycoplasms, Chlamydia and obligatory anaerobes.
  • bacteria e.g. selected from Staphylococci, Streptococci, Enterococci, Mycobacterium , e.g. Mycobacterium tuberculosis ; e.g. and of diseases mediated by Mycoplasms, Chlamydia and obligatory anaerobes.
  • the present Invention provides a method of treatment of microbial diseases, for example of diseases mediated by bacteria, e.g. selected from Staphylococci, Streptococci, Enterococci, Mycobacterium , e.g. Mycobacterium tuberculosis ; e.g. and of diseases mediated by Mycoplasms, Chlamydia and obligatory anaerobes, which method comprises administering to a subject in need of such treatment an effective amount of a noveel compound of the present invwention, e.g. including a compound of formula I P ; e.g. in the form of a pharmaceutical composition.
  • a noveel compound of the present invwention e.g. including a compound of formula I P ; e.g. in the form of a pharmaceutical composition.
  • Treatment includes treatment and prophylaxis.
  • an indicated daily dosage is in the range from about 0.05 g to about 5.0 g, of a novel compound of of the present invention; conveniently administered, for example, in divided doses up to four times a day.
  • a novel compound of the present invention may be administered by any conventional route, for example enterally, e.g. including nasal, buccal, rectal, oral administration; parenterally, e.g. including intravenous, intramuscular, subcutanous administration; or topically; e.g. including epicutaneous, intranasal, intratracheal administration; e.g. in form of coated or uncoated tablets, capsules, injectable solutions or suspensions, e.g. in the form of ampoules, vials, in the form of creams, gels, pastes, inhaler powder, foams, tinctures, lip sticks, drops, sprays, or in the form of suppositories.
  • enterally e.g. including nasal, buccal, rectal, oral administration
  • parenterally e.g. including intravenous, intramuscular, subcutanous administration
  • topically e.g. including epicutaneous, intranasal, intratracheal administration
  • injectable solutions or suspensions e.g.
  • novel compounds of the present invention may be administered in the form of a pharmaceutically acceptable salt, e.g. an acid addition salt or metal salt; or in free form; optionally in the form of a solvate.
  • a pharmaceutically acceptable salt e.g. an acid addition salt or metal salt
  • the novel compounds of the present invention in the form of a salt exhibit the same order of activity as the novel compounds of the present invention in free form; optionally in the form of a solvate.
  • a novel compound of the present invention may be used for pharmaceutical treatment according to the present invention alone, or in combination with one or more other pharmaceutically active agents.
  • Such other pharmaceutically active agents e.g. include other antimicrobials, e.g. including antibiotics, e.g. cephalosporins, penicillins, erythromycins, tetracyclines.
  • a pleuromutilin of to the present invention in diseases mediated by Mycobacterium , but appropriate other pharmaceutically active agents includes agents known to be active in the treatment of diseases mediated by Mycobacterium , such as Rifampicin (Rifampicin®), Streptomycin (Streptomycin®), Ethambutol (Ethambutol®), Pyrizinamid (Pyrizinamid®).
  • Combinations include fixed combinations, in which two or more pharmaceutically active agents are in the same formulation; kits, in which two or more pharmaceutically active agents in separate formulations are sold in the same package, e.g. with instruction for co-administration; and free combinations in which the pharmaceutically active agents are packaged separately, but instruction for simultaneous or sequential administration are given.
  • the present invention provides a pharmaceutical composition
  • a pharmaceutical composition comprising a novel compound of the present invention, e.g. a compound of formula I P , in association with at least one pharmaceutical excipient, e.g. appropriate carrier and/or diluent, e.g. including fillers, binders, disintegrators, flow conditioners, lubricants, sugars and sweeteners, fragrances, preservatives, stabilizers, wetting agents and/or emulsifiers, solubilizers, salts for regulating osmotic pressure and/or buffers; e.g. further comprising another pharmaceutically active agent.
  • a pharmaceutical excipient e.g. appropriate carrier and/or diluent, e.g. including fillers, binders, disintegrators, flow conditioners, lubricants, sugars and sweeteners, fragrances, preservatives, stabilizers, wetting agents and/or emulsifiers, solubilizers, salts for regulating
  • composition comprising pleuromutilins of the present invention for administration in diseases mediated by Mycobtarium may comprise similar excipient as described above.
  • the present invention provides a pharmaceutical composition
  • a pharmaceutical composition comprising a pleuromutilin of the present invention in association with at least one pharmaceutical excipient, and further comprising another pharmaceutically active agent useful in the treatment of Mycobacterium , e.g. M. tuberculosis , infections, such as Rifampicin (Rifampicin®), Streptomycin (Streptomycin®), Ethambutol (Ethambutol®), Pyrizinamid (Pyrizinamid®).
  • Rifampicin Roseptomycin
  • Ethambutol Ethambutol
  • Pyrizinamid Pyrizinamid
  • compositions may be manufactured according, e.g. analogously to a method as conventional, e.g. by mixing, granulating, coating, dissolving or lyophilzing processes.
  • Unit dosage forms may contain, for example, from about 0.5 mg to about 1500 mg, such as 1 mg to about 500 mg.
  • Pleuromutilins of the present invention for administration in diseases mediated by Mycobtarium tuberculosis may be administered in a similar mode and in similar dosages as Rifampicin or Streptomycin.
  • a pleuromutilin of the present invention is preferably selected from the group consisting of a compound of formula I—U.S. Pat. No. 4,278,674, a compound of formula I—EP0153277, a compound of formula I—WO0109095, a compound of formula I—WO0204414, a compound of formula I—WO0222580, a compound of TABLE 1, a compound of formula I B , or a of formula I D ; e.g. including
  • Activity against strains of Mycobacterium e.g. M. tuberculosis may be determined according to the following General Test Procedure:
  • Agar is used as a substrate. Shortly before solidifaction of the Agar TEST COMPOUNDS In different concentrations are added and mixed into the still liquid agar mass (according to the Agar dilution test). Controls without TEST COMPOUNDS are also prepared for determination of strain growth ability. The thus prepared agars are inoculated after solidification with Mycobacterium tuberculosis strains. Incubation is carried out in normal incubators at 37° C. As a nutrition medium Middlebrook 7H10+OADC (Oleic, Albumin, Dextrose, Catalase) Enrichment (pH 6.71-6.73) is used.
  • the minimum inhibition concentration (MIC) which is the compound concentration in the agar which inhibits 99% of strain growth, is determined after 3 weeks, 4 weeks and 5 weeks after inoculation.
  • Pleuromutilins of the present invention show activity against strains of Mycobacterium , e.g. M. tuberculosis and are thus useful in the treatment of infectios caused by Mycobacterium .
  • Pleuromutilins of the present invention surprisingly are even active against resistant and multiresistant M. tuberculosis strains, e.g. strains which are resistant against treatment with known pharmaceuticals useful in the treatment of tuberculosis, e.g. Isoniacid, Rifampicin, Streptomycin.
  • IBB 14-O-[(N-(3-Methyl-2(R)-amino-butyryl)-piperidine-3(S)-yl)-sulfanylacetyl]-2(S)-fluoro-mutilin in the form of a hydrochloride
  • Example I-G is obtained analagosouly to the method of Example I-G, but using a 1:1 mixture of 14-O-[3-(R*)-((N-BOC-(R)-valyl-amino-cyclohexan-1-(S*)-yl)sulfanyl)-acetyl]mutilin and 14-O-[3-(S*)-((N-BOC-(R)-valyl-amino-cyclohexan-1-(R*)-yl sulfanyl)-acetyl]mutilin as a starting material.
  • 1 H-NMR(d 6 -DMSO) Rotamers.
  • a solution of 1.06 g of pleuromutilin-22-O-tosylate dissolved in 10 ml THF is slowly added to a solution of 420 mg of N-BOC-(4-(R/S)-azepane-thiol and 220 mg of potassium-tert.butylate in 25 ml of THF and the mixture obtained is stirred for 2 hours.
  • the mixture obtained is distributed between brine and EtAc, the mixture obtained is extracted with 0.1N HCl, the organic phase obtained is dried, solvent is evaporated and the evaporation residue obtained is subjected to chromatography.
  • Example 43 is obtained analagously to the method of Example IAB), starting from 4-(R/S)-mercapto-azepan-2-one. Characterisation data see TABLE 5, Example 43.
  • 14-O-[((2-(R*)-(N-BOC-(R)-Valyl)-amino-cyclohexan-1-(R*)-yl)-sulfanyl acetyl)]-mutilin is obtained.
  • the BOC-protecting group is cleaved by treatment with 5 ml of etheric HCl and 14-O-[(2-(R*)-((R)-valyl)-amino-cyclohexan-1-(R*)-yl)-sulfanyl acetyl)]-mutilin in the form of a hydrochloride is obtained.
  • a solution of 4.7 g of Na 2 S 2 O 5 in 25 ml of H 2 O is added to a solution of 12.2 g of 14-O-[(carbamimidoylsulfanyl)acetyl]mutilin-tosylate in a mixture of 20 ml of EtOH and 35 ml of H 2 O (warmed to ca. 90°).
  • 100 ml of CCl 4 are added to the reaction mixture obtained and the mixture obtained is heated under reflux for ca. 2 hours.
  • the two-phase system obtained is separated, the organic phase is dried and solvent is evaporated. 14-mercapto-acetyl-mutilin is obtained.
  • N-BOC-3-(R)-hydroxy-piperidine A solution of 2.2 g of N-BOC-3-(R)-hydroxy-piperidine in 10 ml of THF is added under argon and 1 ml of thiolacetic acid to a solution of 3.4 g of triphenylphosphine and 2.65 ml of azadicarbonic acid-isopropylate in 10 ml of THF.
  • the mixture obtained is kept for 18 hours at 70°, solvent is evaporated and the evaporation residue obtained is subjected to chromatography.
  • N-BOC-3-(S)-thioacetoxy-piperidine is obtained.
  • Toluene-4-sulfonic acid (3S, 3aS, 6R, 6aR)-6-hydroxy-hexahydro-furo[3,2-b]furan-3-yl ester (a) and toluene-4-sulfonic acid (3R, 3aS, 6S, 6aR)-6-hydroxy-hexahydro-furo[3,2-b]furan-3-yl ester (b) are obtained.
  • tuberculosis Isoniacid Rifampicin Streptomycin Strain 1 S S S Strain 2 S S S Strain 3 R R S Strain 4 R R R Strain 5 R R S Strain 6 S S Strain 7 R S R Strain 8 S R S Strain 9 R S R Strain 10 S S S Strain 11 S S S Strain 12 S S Strain 13 S S S Strain 14 S S S Strain 15 S S S Strain 15 is the labor strain 137 kV. Resistant and sensible strains are isolated from patients with known sensibilities/resistance. A strain is designated as resistant if its MIC in testing according to the General Test Procedure after 3 to 5 weeks is higher than 20 ⁇ g/ml.
  • TEST COMPOUNDS Activity of TEST COMPOUNDS (TCs) against M. tuberculosis strains 1 to 5 and 7 as set out in TABLE A Is determined in the Agar Dilution Test under conditions as in Example A in different agar concentrations of the TEST COMPOUNDS. The MIC is determined after 3, 4 and 5 weeks.
  • a compound of formula I-Tiamulin TC-1
  • a compound of formula I-Valnemulin TC-2
  • a compound of formula I-PREF1 TC-3
  • a compound of formula I-PREF2 TC-4
  • a compound of formula I-PREF3 TC-5
  • a compound of formula I-PREF4 TC-6
  • a compound of formula I-PREF5 TC-7
  • a compound of formula I-PREF6 TC-8
  • Example B Is carried out according to the method of Example B. Test results obtained are as set out in TABLE C and in TABLE D and in TABLE E: TABLE C MIC ( ⁇ g/ml) against Mycobacterium tuberculosis of strain number TC/week 1 2 3 4 5 6 7 8 TC-2/3 0.5 4 2 8 2 4 4 4 TC-2/4*) 0.5 4 2 8 4 4 8 8 TC-4/3 1 4 1 4 2 4 2 2 TC-4/4 2 4 1 4 2 4 4 2 TC-4/5 2 4 1 4 2 4 4 4 *)Tested after 31 days and thus no further testing after 5 weeks.
  • TC-number indicates the TEST COMPOUND as defined above, e.g. TO-1 indicates a compound of formula I—Tiamulin; and “/week” indicates the MIC-determination point (in weeks from inoculation) in the testing of such TEST COMPOUND.
  • TC-1/3 indicates that the MIC of a compound of formula I—Tiamulin was determined after 3 weeks from inoculation.
  • MIC is the minimum inhibition concentration as defined above.
  • the strain numbers 1 to 4 and 6 to 7 indicated In TABLE B, the strain numbers 1 to 9 indicated in TABLE C and the strain numbers 10 to 15 indicated in TABLE D, and the strain numbers 1, 12 and 15 indicated in TABEL E refer to the corresponding Mycobacterium tuberculosis strains of TABLE 6 R EX R EX R EX Compound I EX Compound I EX Compound I EX Compound I EX Compound I′ EX

Abstract

A method of preventing or treating diseases caused by Mycobacterium, comprising administering to a subject in need of such treatment an effective amount of a pleuromutilin.

Description

  • The present invention relates to tuberculosis treatment, such as treatment of diseases mediated by Mycobacterium, e.g. Mycobacterium tuberculosis, with pleuromutilins.
  • Tuberculosis is a chronic infectious disease mediated by infection with Mycobacterium tuberculosis. Tuberculosis is a major disease in developing countries, as well as an increasing problem in developed areas of the world. Although the infection may be asymptomatic for a considerable period of time, the disease is most commonly manifested as an acute inflammation of the lungs, resulting in fever and a nonproductive cough. If untreated, serious complications and death typically result. Tuberculosis may be generally controlled by antibiotic therapy, such as by treatment with Isoniazid, see e.g. The Merck Index, 12th edition, item 5203; Rifampin (Rifampicin®), see e.g. The Merck Index, 12th edition, item 8382, Streptomycin, see e.g. The Merck Index, 12th edition, item 8983; but a major problem is the development of strain drug resistance against such antibiotics.
  • We have now found a compound class which shows surprisingly activitiy in the treatment of diseases caused by Mycobacterium, such as Mycobacterium tuberculosis, e.g. even against drug resistant strains.
  • In one aspect the present invention provides the use of a pleuromutilin in the preparation of a medicament for the treatment of diseaeses mediated by Mycobacterium.
  • In another aspect the present invention provides a method of treating diseases mediated by Mycobacterium, comprising administering to a subject in need of such treatment an effective, e.g. an anti-mycobacterium effective; amount of a pleuromutilin.
  • Mycobacterium includes M. tuberculosis. Diseaeses mediated by Mycobacterium include mycobacterium infections. A pleuromutilin for treatment includes one or more pleuromutilins, e.g. a combination of different pleuromutilins. Treatment includes treatment and prophylaxis. A pleuromutilin for use according to the present invention or for treating diseases according to the present invention is designated hereinafter as “a pleuromutilin(s) of (according to) the present invention”.
  • A pleuromutilin of the present invention includes a pleuromutilin in the form of a free base, and, where existing, in the form of a salt, in the form of a solvate and in the form of a salt and a solvate, e.g. and in the form of a complex, such as a cyclodextrin complex. A pleuromutilin of the present invention may exist in the form of isomers and mixtures thereof, e.g. including diastereoisomers and mixtures thereof. Isomeric mixtures may be separated as appropriate, e.g. according to a method as conventional, to obtain pure isomers. The present invention includes a pleuromutilin according to the present invention in any isomeric form and in any isomeric mixture, such as described in patent literature cited below, which patent literature is introduced herein by reference with respect to isomeric forms of pleuromutlins. Preferably the cofiguration in the mutilin ring is the same as in a naturally produced mutilin.
  • Pleuromutilin, a compound of formula
    Figure US20050215637A1-20050929-C00001
    is a naturally occurring antibiotic, e.g. produced by the basidomycetes Pleurotus mutilus and P. passeckerianus, see e.g. The Merck Index, 12th edition, item 7694.
  • A number of further pleuromutilins having the principle ring structure of pleuromutilin and having e.g. antibacterial activity, have been developed.
  • A pleuromutilin of the present invention includes a pleuromutilin having the basic structural elements as set out in formula
    Figure US20050215637A1-20050929-C00002
    wherein R is vinyl or ethyl and the dotted line is a bond or is no bond.
  • The following numbering system is used in the present application:
    Figure US20050215637A1-20050929-C00003
  • The dotted line between positions 19 an 20 (and between positions 1 and 2) is a bond or is no bond. In a compound of formula A or of formula PLEU a hydrogen atom in positions 4, 7 and/or 8 of the ring system may be replaced by deuterium, and if the dotted line between positions 1 and 2 is no bond (single bond between positions 1 and 2) the ring system may be further substituted in positions 1 and/or 2, e.g. by halogen, deuterium or hydroxy. The group —O— in position 14 is further substituted, preferably by a substituted carbonyl group.
  • Examples of pleuromutilins according to the present invention includes e.g.
      • A compound as disclosed in U.S. Pat. No. 3,716,579, e.g. of formula
        Figure US20050215637A1-20050929-C00004
        wherein R is CH3—(CH2)7—CH═CH—(CH2)7—COO—, CH3—(CH2)4—CH═CH—CH2—CH═CH—(CH2)7—COO—, CH3—(CH2)9—CH═CH—(CH2)7—COO— or hydrogen;
      • A compound as disclosed in GB1312148, e.g. of formula
        Figure US20050215637A1-20050929-C00005
        wherein X, Y and Z are as defined in any one of the following groups:
    • a. X is —CO—CH2—R1, wherein R1 is H, Cl, Br, I, thiocyanato, azido, (N,N-tetramethylene-thlocarbamoyl)-mercapto, dithiocarbonic acid-O—(C1-3)alkyl, —S-phenyl, S-phenyl substituted by carboxyl or by one or two OH, —S-pyridyl, S-benzyl, —S—(C1-5)alkyl, or —S—(C1-5)alkyl substituted by one or more amino, OH or carboxyl, Y is vinyl, and Z is H;
    • b. X is —CO—CO—OH, Y is vinyl and Z is H;
    • c. X Is —COCH3, Y is vinyl and Z is H;
    • d. X is COCH2NH2, Y is ethyl and Z is H;
    • e. X is a group of formula
      Figure US20050215637A1-20050929-C00006
    • f. X is H, Y is vinyl and Z is acetyl; or
    • g. X is COR2, wherein R2 is (C1-5)alkyl, Y is vinyl and Z is H,
      • A compound as disclosed in U.S. Pat. No. 4,278,674, e.g. of formula
        Figure US20050215637A1-20050929-C00007
        wherein R1 is vinyl or ethyl, n is an integer from 2 to 5, x is sulphur or a group —Y-phenylene-Z- or a group ═NR4, Y and Z are both sulphur or one of Y and Z is sulphur and the other is oxygen, R4 is H or a second mutilin ring of formula I—U.S. Pat. No. 4,278,674, wherein R1 is as defined above and attached via a —O—CO—CH2— group in position 14; each of R2 and R3 are (independently of each other) (C1-10)alkyl, or R2 and R3 together with the nitrogen atom form pyrrolidino, piperidino, morpholino, thiomorpholino, or 1-hexahydro-1H-azepino, or R2 and R3 together with the nitrogen atom form piperazinyl, the second nitrogen atom of which is substituted by (C1-5)alkyl, (C1-4)hydroxyalkyl, (C2-5)alkynoyloxy(C1-4)alkyl, or benzoyloxy(C1-4)alkyl, or
    • R1 is as defined above, n=2, R3 is (C1-10)alkyl, (C1-4)hydroxyalkyl, (C2-5)alkynoyloxy-(C1-4)alkyl, or benzoyloxy(C1-4)alkyl, X is ═NR′4 and R2 together with R′4 forms an ethylene bridge between both nitorgen atoms; such as
    • 14-Desoxy-14[(2-diethylaminoethyl)mercaptoacetoxy]mutilin, e.g. also known as tiamulin of formula
      Figure US20050215637A1-20050929-C00008
      • A compound as disclosed in U.S. Pat. No. 4,130,709, e.g. of formula
        Figure US20050215637A1-20050929-C00009
        wherein R is ethyl or vinyl, R1 is selected from α- or β-anomers of hexopyranoses, hexofuranoses, pentopyrancses, pentofuranoses, pyranose and furanose aminosugars, disaccharides, trisaccharides R2 is H, benzoyl or (C2-4)alkanoyl; or R1 is 2-deoxy-2-(hydroxyimino)-3,4,6-tri-O-acetyl-α-D-glucopyranosyl or -galactopyranosyl, 2-deoxy-2-(hydroxyimino)-α-D-galactopyranosyl, 2-deoxy-2-amino-4,6-di-O-acetyl-α-D-glucopyranosyl, or 2-deoxy-2-acetamido-3,4,6-tri-O-acetyl-α-D-glucopyranosyl and R2 is H;
      • A compound as disclosed in U.S. Pat. No. 4,129,721; e.g. of formula
        Figure US20050215637A1-20050929-C00010
        and the 19,20-dihydro derivative thereof and the tetra (C2-6)alkanoyl derivatives thereof;
      • A compound as disclosed in EP0013768, e.g. of formula
        Figure US20050215637A1-20050929-C00011
        wherein R1 is vinyl or ethyl, m is 0 or 1, and R2 is a heterocyclic radical, in which a 5- or 6-membered, unsaturated or saturated heterocyclic ring containing one or more hetero atoms selected from O, S and N, is attached to the —S(CH2)m— group;
      • A compound as disclosed in EP0153277, e.g. an N-acyl-14-O-[(1-amino-2-methylpropan-2-yl)thioacetyl]-mutilin or 19,20-dihydromutilin, such as of formula
        Figure US20050215637A1-20050929-C00012
        wherein R1 is vinyl or ethyl positions 19 and 20), and R2 Is optionally hydroxy-substituted aminoalkyl or a 5-membered saturated heterocycle, e.g. including Valnemulin (Econor®) of formula
        Figure US20050215637A1-20050929-C00013
      • A compound as disclosed in U.S. Pat. No. 516,526, e.g. of formula
        Figure US20050215637A1-20050929-C00014
        wherein R1 and R2 independently of each other are H, alkyl, alkenyl, cycloalkyl, aryl or aralkyl;
      • A compound as disclosed in WO9322288, e.g. of formula
        Figure US20050215637A1-20050929-C00015
        wherein R1 and R2 are independently of each other H, alkyl, or, R1 and R2 together with the carbon atom to which they are attached are cycloalkyl; and R3 and R4 independently of each other are H, alkyl or substituted alkyl;
      • A compound as disclosed in WO9725309, e.g. of formula
        Figure US20050215637A1-20050929-C00016
        wherein Y is carbamoyloxy, wherein the N-atom is unsubstituted or mono- or disubstituted, such as a compound of formula
        Figure US20050215637A1-20050929-C00017
        wherein R1 is vinyl or ethyl, R2 and R3 independently of each other are H, or optionally substituted
      • saturated or unsaturated (C1-6) hydrocarbon or (C3-8)cyclic hydrocarbon,
      • heterocyclyl or aryl, or
    • R2 and R3 together form an optionally substituted cyclic group of 3 to 8 ring atoms, optionally containing one additional heteroatom selected from N, O and S, and optionally fused to a hydrocarbon ring, a heterocyclic group or an aromatic group; or
    • R2 is one of the above monovalent groups and R3 is a group selected form SO2R4, COR5, OR5 and NR6R7; wherein
    • R4 is optionally substituted,
      • saturated or unsaturated (C1-6)hydrocarbon or (C3-8)cyclic hydrocarbon,
      • heterocyclyl, aryl, (C1-6)alkylamino or arylamino;
    • R5 is optionally substituted
      • saturated or unsaturated (C1-6) hydrocarbon or (C3-8)cyclic hydrocarbon,
      • heterocyclyl or aryl,
    • R6 and R7 independently of each other are H, or optionally substituted
      • saturated or unsaturated (C1-6) hydrocarbon or (C3-8)cyclic hydrocarbon,
      • heterocyclyl or aryl, or
    • R6 and R7 together with the nitrogen atom to which they are attached form an optionally substituted (C3-8)cyclic group, optionally containing one additional heteroatom selected from N, O or S, and optionally fused to a hydrocarbon ring, a heterocyclic ring or an aromatic group;
      • A compound as disclosed in WO9805659, e.g. of formula
        Figure US20050215637A1-20050929-C00018
        wherein R1 is vinyl or ethyl, and R2 is a group R3, R4CH2—, or R5R6CH═CH—, wherein,
    • each of R3 and R4 is an azabicyclic ring system, or R5 and R6 together with the carbon atom to which they are attached form an azabicyclic ring system;
      • A compound of WO9821855; e.g. of formula
        Figure US20050215637A1-20050929-C00019
        wherein n and m are independently of each other 0, 1 or 2; X is O, S, S(O), SO2, —COO—, —NH—, —CONH—, —NHCONH—, or a bond; R1 is vinyl or ethyl; R2 is a non-aromatic monocyclic or bicyclic group containing one or two basic nitrogen atoms and attached through a ring carbon atom, e.g. R2 is optionally substituted quinuclidinyl, azabicyclo[2.2.1]heptyl, azabicyclo[4.3.0]nonyl, azabicyclo[3.2.1]octyl, azabicyclo[3.3.0]octyl, azabicyclo[2.2.2]octyl, azabicyclo[3.2.1]octenyl, azabicyclo[3.3.1]nonyl or azabicyclo[4.4.0]decyl; R3 is H, OH; or the moietity R2(CH2)mX(CH2)nCH2COO at position 14 of IA or IB is replaced by RaRbC═CHCOO, wherein one of Ra or Rb is hydrogen and the other is R2; or Ra and Rb together form R2;
      • A compound as disclosed in WO0007974, e.g. a 14-acyloxy derivative of mutilin or 19,20-dihydromutilin having a 2-fluorosubstituent, such as of formula
        Figure US20050215637A1-20050929-C00020
        wherein R1 is vinyl or ethyl (positions 19 and 20), and R2COO— is acyloxy, e.g. HOCH2CO2—or R—X—CH2CO2, wherein X is O, S or NR′ and R and R′ are indpendently of each other an aliphatic or aromatic group, preferably R2COO— is a carbamoyl group, such as a group R3R4NCO2— wherein R3 and R4 have various meanings (e.g. R3 and R4 have the meaning as disclosed for the meaning of R2 and R3 in WO9725309);
      • A compound as disclosed in WO0027790, e.g. a compound of formula
        Figure US20050215637A1-20050929-C00021
        wherein R1 is a RA(CH2)nO(CH2)m, RA(CH2)p, or a group of formula
        Figure US20050215637A1-20050929-C00022
        wherein R is a spiro-fused mono- or bicyclic ring containing one or two basic N-atoms; X1 and X2 which may be the same or different, are each —CH2— or —C═O, provided that at least one of X1 and X2 is —C═O; and Y is —NH—, —CH2— or —CH2—CH2—; RA is an optionally substituted aryl group or heteroaryl group linked via a carbon atom; e.g. RA is optionally substituted phenyl, thienyl, pyridinyl, furyl, thiazolyl, isoxazolyl, benzimidazolyl, quinolinyl, 1,2,3,4-tetrahydro-isoquinolinyl or benzthiazolyl:
    • m is 1, 2 or 3; n is 0, 1 or 2; p is 1 to 4; R2 is vinyl or ethyl; and R3 is H, OH or F, and is H; or R3 is H and R4 is F;
      • A compound as disclosed in WO0037074, e.g. a compound of formula
        Figure US20050215637A1-20050929-C00023
        wherein R1 is an optionally substituted heteroaryl group which comprises a 5-membered heteroaromatic ring which has at least one N-atom, e.g. a pyrrole, pyrazole, imidazole, 1,2,3-triazole, 1,2,4-triazole, indole, benzimidazole, benzotriazole, 2-aza-indole or 6-aza-indole; and which is linked via a N-atom; R2 is vinyl or ethyl; R3 is H, OH or F, and R4 is H; or R3 is H and R4 is F;
      • A compound as disclosed in WO0073287, e.g. a compound of formula
        Figure US20050215637A1-20050929-C00024
        wherein R1 is optionally substituted aryl, e.g. azabicyclo-octyl; or an optionally substituted nitrogen containing ring, e.g. piperidinyl; R2 is vinyl or ethyl; R3 is H, OH or F and R4 is H; or R3 is H and R4 is F;
      • A compound as disclosed in WO0114310, e.g. a compound of formula
        Figure US20050215637A1-20050929-C00025
        wherein R1 is a nitrogen containing heterocycle, an optionally substituted aryl or optionally subsituted heteroaryl, or CH2N,
    • e.g. R1 is optionally substituted phenyl, 3-pyridyl, 4-pyridyl, pyrimidin-2-yl, 1,3,4-thiadiazol-2-yl, benzothiazol-2-yl, 2H-1,2,4-triazol-3-yl, azabicycloheptyl, azabicydooctyl or piperidinyl; R2 is vinyl or ethyl; R3 is H, OH or F and R is H; or R3 is H and R is F; R5 is halogen or SR6; and R6 is aminoalkyl, a nitrogen containing heterocycle, or an optionally substituted aryl or optionally subsituted heteroaryl; e.g. R6 is optionally substituted phenyl, 3-pyridyl, 4-pyridyl, pyrimidin-2-yl, 1,3,4-thiadiazol-2-yl, benzothiazol-2-yl. 2H-1,2,4-triazol-3-yl, azabicycloheptyl, azabicyclooctyl or piperidinyl;
      • A compound as disclosed in WO0109095, e.g. a compound of formula
        Figure US20050215637A1-20050929-C00026
        wherein R is hydrogen or alkyl; R1 Is hydrogen or a group of formula
        Figure US20050215637A1-20050929-C00027
        wherein X is S, O, or NR10, wherein R10 is H or alkyl, or N+(R′10)2 wherein R′10 is alkyl in the presence of an appropriate anion; and R9 is amino, alkyl, aryl, heterocyclyl or mercapto; and, if X is oxygen, R9 is additionally hydrogen; R2 is arylene, e.g. phenylene; or heterocyclene; R4 is hydrogen or alkyl; R5 is hydrogen or alkyl; R3, R3′, R6, R7 and R8 independently of each other are hydrogen or deuterium; or R and R2 together with the nitrogen atom to which they are attached form non-aromatic heterocyclene and R1 is a group of formula
        Figure US20050215637A1-20050929-C00028
        wherein X and R9 are as defined above; e.g. a compound of formula
        Figure US20050215637A1-20050929-C00029
        wherein R1s is hydrogen or a group of formula
        Figure US20050215637A1-20050929-C00030
        wherein R5s is hydrogen or deuterium; R2s is hydrogen, methyl or tert-butyl; R7s is hydrogen or methyl; and R3s, R4s and R5s are hydrogen or deuterium;
      • A compound as disclosed in WO0174788, e.g. a compound of formula
        Figure US20050215637A1-20050929-C00031
        wherein R1 is a 5- or 6-membered optionally substituted heteroaryl group;
    • e.g. pyridine, pyridazine, pyrimidine, pyrazine, isoxazole, thiazole, imidazole, pyrazole, 1,2,3-triazole, 1,2,4-triazole, benzimidazole, 3-oxo-3,4-dihydropyrido[2,3-b]pyrazine, or pyrazolo[1,5-a]pyrimidine; and R2 is vinyl or ethyl;
      • A compound as disclosed in WO0204414, e.g. a compound selected from 14-O-[(cycloalkyl-sulfanyl)acetyl]mutilins; 14-O-[(cycloalkyl-alkyl-sulfanyl)acetyl]mutilins; 14-O-[(cycloalkoxy)acetyl]mutilins; or 14-O-[(cycloalkyl-alkoxy)acetyl]mutilins, such as of formula
        Figure US20050215637A1-20050929-C00032
        wherein R is hydrogen; R1 is hydrogen or a group of formula
        Figure US20050215637A1-20050929-C00033
        wherein X is sulphur, oxygen or NR10, wherein R10 is hydrogen or alkyl; and R9 is amino, alkyl, aryl or heterocyclyl; and, if X is oxygen, R9 is, additionally hydrogen; Y is sulphur or oxygen; R2 is hydrogen or one or more substituents, R4 is hydrogen or alkyl; R5 is hydrogen or alkyl; R3 and R3′ are hydrogen, deuterium, or halogen; R6, R7 and R8 are hydrogen or deuterium; m is a number selected from 0 to 4; n is a number selected from 0 to 10; and p is a number selected from 0 to 10; with the proviso that n plus p are at least 1; e.g. a compound of of formula
        Figure US20050215637A1-20050929-C00034
        wherein R1p is hydrogen or the residue of an amino acid;
      • A compound as disclosed in WO0212199, e.g. a compound of formula
        Figure US20050215637A1-20050929-C00035
        wherein R1 is:
      • a 5- or 6-membered aromatic or heteroaromatic ring attached via a ring carbon atom, preferably pyridyl, and comprising a substituent selected from halo, R7O—, R7S— or R8R9N— on a ring carbon adjacent to the carbon of attachment; or
      • a 5- or 6-membered dihydro heteroaromatic ring attached via a ring carbon atom and comprising one oxygen or one or two nitrogen atoms and optionally fused to phenyl, a 5- or 6-membered heteroaryl ring comprising one or two nitrogen atoms or a 5- or 6-membered heterocyclyl ring comprising a sulphur, oxygen or nitrogen atom and further comprising a substituent selected from oxo or thioxo on a ring carbon adjacent to the carbon of attachment;
      • a 6-membered tetrahydro heteroaromatic ring attached via a ring carbon atom comprising one or two nitrogen atoms and further comprising two substituents independently selected from oxo or thioxo wherein one of the substituents is on a ring carbon adjacent to the carbon of attachment; or
      • a bicyclic heteroyaryl ring attached via a ring carbon atom and comprising nine or ten ring atoms and from one to four nitrogen atoms;
        wherein the ring of R1 may be optionally further substituted; R2 is vinyl or ethyl; R3 is H, OH or F and R4 is H, or R3 is H and R4 is F; and R5 and R6 together form an oxo group; or R3 and R4 is each H and R5 is H, or OH and R6 is H, or R5 is H and R6 is H or OH; R7 is optionally substituted (C1-6)alkyl; and R8 and R9 are independently selected from hydrogen or optionally substituted (C1-6)alkyl.
      • A compound as disclosed in WO0222580, of formula
        Figure US20050215637A1-20050929-C00036
        wherein R and R2 together with the nitrogen atom to which they are attached form pyrrolidinyl or piperidinyl, R1 is a group of formula
        Figure US20050215637A1-20050929-C00037
        R3 and R3′ are hydrogen, deuterium or halogen, R4 is hydrogen or alkyl, R5 is hydrogen or alkyl, R6, R7 and R8 are hydrogen or deuterium; R9 is amino, alkyl, aryl, heterocyclyl or mercapto; and, if X is oxygen, R9 is additionally hydrogen; R10 is hydrogen or alkyl, R10′ is alkyl, X is sulphur, oxygen, NR10, or N+(R10′)2 in the presence of an appropriate anion, Y is sulphur or oxygen, and m is 0, 1 or 2;
        with the proviso that, when R and R2 together with the nitrogen atom to which they are attached form piperidinyl, m is 0, Y is S and Y is attached in position 3 of said piperidine ring that group of formula I which is attached to the piperidine ring via the residue Y is either in the (S)-configuration or in the (R)-configuration, preferably in the (S)-configuration; preferably a compound of of formula
        Figure US20050215637A1-20050929-C00038
        wherein R3p, R3p′, R6p, R7p and R8p are, index-number correspondingly, as defined for a compound of formula I—WO0222580 for R3, R3′, R6, R7 and R8; and R5p is hydrogen or one or more substituents, and if the group attached to the piperidine ring via the sulphur atom is in position 3 of said piperidine ring and R5p is hydrogen, then the group attached to the sulphur atom is either in the (S)-configuration or in the (R)-configuration;
        a compound of formula
        Figure US20050215637A1-20050929-C00039
        wherein R3q, R3q′, R6q, R7q and R8q are, index-number correspondingly, as defined for a compound of formula I—WO0222580 for R3, R3′, R6, R7 and R8; R5q is hydrogen or one or more substituents, preferably hydrogen; and Rq is that part of an amino acid which remains if the carboxylic group is splitt off;
        a compound of formula
        Figure US20050215637A1-20050929-C00040
        wherein R3r, R3r′, R4r, R6r, R7r and N8r are, index-number correspondingly, as defined for a compound of formula WO0222580 for R3, R3′, R4, R6, R7 and R8; R5r is hydrogen or one or more substituents, and R1r is that part of an amino acid which remains if the carboxylic group is splitt off, or a compound of formula
        Figure US20050215637A1-20050929-C00041
        wherein R3s, R3s′, R4s, R6r, R7s and R8s, respectively, are, index-number correspondingly, as defined for a compound of formula I—WO0222580 for R3, R3′, R4, R6, R7 and R8;
    • R5 is hydrogen or one or more substituents, preferably hydrogen; and R1s is that part of an amino acid which remains if the carboxylic group is splitt off; e.g. wherein in a compound of formula Is the group attached to the piperidine ring via the sulphur atom is either in the (S)-configuration or in the (R)-configuration; e.g. wherein in a group R1s the amine group of the amino acid residue is either in the (S)-configuration or in the (R)-configuration.
  • Furthermore we have found novel pleuromutilins which show antibacterial activity and activity against Mycobacterium tuberculosis.
  • In another aspect the present invention provides a pleuromutilin, which is selected from the group consisting of compounds of formulae
    Figure US20050215637A1-20050929-C00042
  • wherein REX is as set out in TABLE 1. In all Examples of TABLE 1 the compounds are of formula IEX, with the exception of Example 12 in which the compound is of formula I′EX. 1H-NMR-data of the compounds described and obtained according, e.g. analogously, to a method as described in the examples, are also indicated in TABLE 1:
    TABLE 1
    Figure US20050215637A1-20050929-C00043
         		Example 1 14-O-[4-Amino-cyclohexan-1-yl-sulfanyl)-acetyl]-mutilin in the form of a hydrochloride (d6-DMSO): 7.9(b, 3H, NH3), AB-system (νA=3.23, νB=3.29, 2H, H22, J=15.2Hz), 3.03(m, 1H, SCH), 3.10(m, 1H, CHN)
    Figure US20050215637A1-20050929-C00044
         		Example 2 14-O-[(2-(R*)-((R)-Histidinyl)-amino-cyclohexan-1-(S*)-yl)-sul- fanyl acetyl)]-mutilin in the form of a hydrochloride (d6-DMSO): Diastereoisomers: 8.4, 9.0(2xm, 2H, NH), 7.5, 8.7(2xb, 2H, imidazole), 6.15, 5.1(2xm, H19, H20, H21), 5.52(d, 1H, J=5.2Hz, H14), 4.2(m, 1H, a-H-histidine)3.45(m, 1H, H11), 3.3(m, 2H, H22), 2.7(m, 1H,
    #CHS), 1.18, 1.45(2xs, (CH3)15, (CH3)18), 0.75, 0.88(2xd, (CH3)16, (CH3)17, J=5.4Hz)
    Figure US20050215637A1-20050929-C00045
         		Example 3a 14-O-[(2-(R*)-Methylamino-cyclohexan-1-(S*)-yl)-sulfanyl acetyl)]-mutilin (d6-DMSO): Diastereoisomers: 6.15, 5.1(2xm, H19, H20, H21), 5.52(d, 1H, J=5, 2Hz, H14), 4.50(d, 1H, OH, J=5Hz), 3.45(t, 1H, H11, J=5Hz), 3.25(m, 2H, H22), 3.25(m, 1H, CHN), 2.82(m, 1H, CHS), 2.38(d, 3H, CH3NH, J=5.1Hz), 1.3, 1.34)1.18,
    #1.45(2xs, (CH3)15, (CH3)18), 0.75, 0.88(2xd, (CH3)16, (CH3)17, J=5.4Hz). Example 3b 14-O-[(2-(R*)-Methylamino-cyclohexan-1-(R*)-yl)-sulfanyl acetyl)]-mutilin (d6-DMSO): Diastereoisomers: 6.15, 5.1(2xm, H19, H20, H21), 5.52(d, 1H, J=5.2Hz, H14), 4.50(d, 1H, OH, J=5Hz), 3.45(t, 1H, H11, J=5Hz), 3.25(m,
    #2H, H22), 3.25(m, 1H, CHN), 2.65(m, 1H, CHS), 2.43(d, 3H, CH3NH, J=5.1Hz), 1.3, 1.34)1.18, 1.45(2xs, (CH3)15, (CH3)18), 0.75, 0.88(2xd, (CH3)16, (CH3)17, J=5.4Hz)
    Figure US20050215637A1-20050929-C00046
         		Example 4 (14-O-[4-((R)-Valyl-amino-cyclohexane-1-yl)-sulfanyl)-ace- tyl]-mutilin in the form of a hydrochloride (CD3OD): 8.3(d, 1H, NH), 8.1(b, 3H, NH3), 6.15(m, 1H, H19), 5.55(d, 1H, H14), 5.05(m, 2H, H20), 3.75(m, 1H, NCHCO), 3.3(m, 1H, NCH), 3.42(d, 1H, H11), 3.25(m, 2H, SCH2CO), 2.98(m, 1HCHS), 0.9(d, 6H, (CH3)2CH), 1.08, 1.36(2xs, 6H,
    #(CH3)18, (CH3)15), 0.65, 0.83(2xd, 6H, (CH3)16, (CH3)17)
    Figure US20050215637A1-20050929-C00047
         		Example 5a 14-O-[((3-(R*)-Amino-cycloheptan-1(R*)-yl)sulfanyl)acetyl]-mu- tilin and 14-O-[((3-(S*)-amino-cycloheptan-1(S*)-yl)-sul- fanyl)-acetyl]mutilin in the form of a hydrochloride (diastereoisomeric mixture) (d6-DMSO): 8.0(b, 3H, NH3 +), 6.15, 5.1(2xm, H19, H20, H21), 5.52(d, 1H, J=5.2Hz, H14), 3.1, 3.2(2xm1H, CHNH3 +),
    #3.4(m, 1H, H11,), 3.3(m, 2H, H22), 2.9(m, 1H, SCH), 1.18, 1.45(2xs, (CH3)15, (CH3)18), 0.9(m, 6H, CH(CH3)2), 0.75, 0.88(2xd, (CH3)16, (CH3)17, J=5.4Hz) Example 5b 14-O-[((3-(R*)-Amino-cycloheptan-1(S*)-yl)sulfanyl)acetyl]-mu- tilin and 14-O-[((3-(S*)-Amino-cycloheptan-1(R*)-yl)sul- fanyl)acetyl]-mutilin in the form of a
    #hydrochloride (diastereoisomeric mixture) (d6-DMSO): 7.8(b, 3H, NH3 +), 6.15, 5.1(2xm, H19, H20, H21), 5.52(d, 1H, J=5, 2Hz, H14), 3.15(2xm1H, CHNH3 +), 3.4(m, 1H, H11,), 3.3(m, 2H, H22), 2.95(m, 1H, SCH), 1.18, 1.45(2xs, (CH3)15, (CH3)18), 0.9(m, 6H, CH(CH3)2), 0.75, 0.88(2xd, (CH3)16,
    #(CH3)17, J=5.4Hz)
    Figure US20050215637A1-20050929-C00048
         		Example 6 14-O-[(3-(R/S)-(R)-Valylamino-1-(R/S)-yl)sul- fanyl)acetyl]mutilin in the form of a hydrochloride (d6-DMSO): 8.4(m, 1H, NHC=O), 8.1(b, 3H, NH3 +), 6.15, 5.1(2xm, H19, H20, H21), 5.52(d, 1H, J=5, 2Hz, H14), 3.5, 3.9(2xm, 1H, a-H-va- lyl), 3.15(2xm1H, CHNH3 +), 3.4(m, 1H, H11), 3.3(m, 2H, H22), 2.95(m, 1H, SCH),
    #1.18, 1.45(2xs, (CH3)15, (CH3)18), 0.9(m, 6H, CH(CH3)2), 0.75, 0.88(2xd, (CH3)16, (CH3)17, J=5.4Hz)
    Figure US20050215637A1-20050929-C00049
         		Example 7 14-O-[(3-(R/S)-Guanidino-cyclohexan-1-(R/S)-yl)-acetyl]mutilin-hydro- chloride (d6-DMSO): 6.8-7.4(b, 3H, NH3 +), 7.65, 7.7(2xm, 1H, NH), 6.15, 5.1(2xm, H19, H20, H21), 5.52(d, 1H, J=5, 2Hz, H14), 4.5(d, 1H, OH, J=6Hz), 3.1(m, 1H, CHNH), 3.4(t, 1H, H11, J=6Hz), 3.3(m, 2H, H22), 2.7(m, 1H, SCH), 1.18, 1.45(2xs,
    #(CH3)15, (CH3)18), 0.75, 0.88(2xd, (CH3)16, (CH3)17, J=5.4Hz)
    Figure US20050215637A1-20050929-C00050
         		Example 8a 14-O-[3-(R*)-((R)-Valyl-amino-cyclopentan-1-(S*)-yl)-sulfanyl)-ace- tyl]-mutilin in the form of a hydrochloride (DMSO-d6): 8.5(d, 1H, NH, J=7.2Hz), 8.1(bs, 3H, NH3 +), 6.15, 5.06, 5.02(3xm, H19, H20, H21), 5.55(d, 1H, H14, J=8.2Hz), 4.05(m, 1H, H-al- pha-valyl), 3.15(m, 1H, H-1′), 3.2-3.5(m, H-3′, H11, H22), 1.35,
    #1.05(2xs, (CH3)15, (CH3)18), 0.91, 0.88(d, (CH3)2CH, J=6.8Hz), 0.8, 0.62(2xd, (CH3)16, (CH3)17, J=6.8Hz) Example 8b 14-O-[3-(S*)-((R)-Valyl-amino-cyclopentan-1-(R*)-yl)-sulfanyl)-ace- tyl]-mutilin in the form of a hydrochloride (DMSO-d6): 8.5(d, 1H, NH, J=7.2Hz), 8.1(bs, 3H, NH3 +), 6.15, 5.06,
    #5.02(3xm, H19, H20, H21), 5.55(d, 1H, H14, J=8.2Hz), 4.05(m, 1H, H□-valyl), 3.15(m, 1H, H-1′), 3.2-3.5(m, H3′, H11, H22), 1.35, 1.05(2xs, (CH3)15, (CH3)18), 0.91, 0.89(d, (CH3)2CH, J=6.9Hz), 0.8, 0.62(2xd, (CH3)16, (CH3)17, J=6.8Hz). Example 8c 14-O-[3-(S*)-((R)-Valyl-amino-cyclo
    #pentan-1-(S*)-yl)-sulfanyl)-ace- tyl]-mutilin and 14-O-[3-(R*)-((R)-valyl-amino-cyclopentan-1-(R*)-yl)-sul- fanyl)-acetyl]-mutilin in the form of a hydrochloride (mixture of trans-diastereoisomers) (DMSO-d6): 8.52, 8.53(2xd, 1H, NH, J=6.9Hz), 8.1(bs, 3H, NH3 +), 6.15, 6.12, 5.0-5.1(6xm, H19, H20, H21), 5.54, 5.55(2xd, 1H, H14, J=8.2Hz), 4.15(m, 1H,
    #H□-valyl), 3.1-3.5(m, H-1′, H3′, H11, H22), 1.35, 1.05(2xs, (CH3)15, (CH3)18), 0.91, 0.88(d, (CH3)2CH, J=6.8Hz), 0.8, 0.62(2xd, (CH3)16, (CH3)17, J=6.8Hz)
    Figure US20050215637A1-20050929-C00051
         		Example 9 14-O-[((3-(R/S)-Amino-cyclopentyl)-sulfanyl)-acetyl]-mutilin-hy- drochloride (DMSO-d6): 8.03(bs, 3H, NH3 +), 6.13, 5.05(2xm, 3H, H19, H20, H21), 5.55(d, 1H, H14, J=8.0Hz), 3.2-3.6(m, H-3′, H11, H22), 3.14(m, 1H, H-1′), 1.35, 1.05(2xs, (CH3)15, (CH3)18), 0.8, 0.62(2xd, (CH3)16,
    #(CH3)17, J=6.8Hz)
    Figure US20050215637A1-20050929-C00052
         		Example 10 14-O-[(2-(R*)-((R)-Valyl)-methylamino-cyclohexan-1-(R*)-yl)-sul- fanyl acetyl)]-mutilin in the form of a hydrochloride (d6-DMSO): Diastereoisomers: 8.0(m, 3H.NH3 +), 6.15, 5.1(2xm, H19, H20, H21), 5.52(d, 1H, J=5, 2Hz, H14), 4.50(m, 1H, OH), 4.2(m, 1H, a-H-val- ine), 3.45(m, 1H, H11), 3.25(m, 2H, H22), 3.25(m, 1H, CHN),
    #2.82(m, 1H, CHS), 2.88, 2.94(2xs, 3H, CH3N), 1.3, 1.34), 1.18, (2xw, (CH3)15, (CH3)18), 0.75, 0.88(2xd, (CH3)16, (CH3)17, J=5.4Hz)
    Figure US20050215637A1-20050929-C00053
         		Example 11 14-O-[(3-Guanidino-phenylsulfanyl)-acetyl]mutilin in the form of a hydrochloride (CDCl3): 0.58(d, 3H, H16, J=7.2Hz), 0.81(d, 3H, H17, J=7.3Hz), 1.02(s, 3H, H18), 1.32(s, 3H, H15), ABX-system(νA=1.2, νB=1.88, H13a, H13b, J=16.1Hz, J=9.1Hz), 2.08(d, 1H, H4, J=2.1Hz),
    #ABXY-sys- tem(νA=2.23, νB=2.19, H2a, H2b, J=16.2Hz, J=9.1Hz, J=1.8Hz), 2.3(m, 1H, H10), 3.4(d, 1H, H11, J=5.98Hz), AB-system(νA=3.81, νB=3.89, 2H, H22, J=14.1Hz), 5.18(dd, 1H, H20a, J=17.5Hz, J=1.6Hz), 5.29(dd, 1H, H20b, J=11Hz, J=1.6Hz), 5.51(d, 1H, H14, J=8.3Hz), 6.05(dd, 1H, H19,
    #J=11Hz, J=17.5Hz), 7.0(m, 1H, arom.H), 71.8(m2H, arom.H), 7.3t, 1H, arom.H5, J=8Hz
    Figure US20050215637A1-20050929-C00054
         		Example 12 14-O-[(N-(3-Methyl-2(R)-amino-butyryl)-piperidine-3(S)-yl)-sul- fanylacetyl]-2(S)-fluoro-mutilin in the form of a hydrochloride (DMSO-d6): Rotamers: 7.95(bs, 3H, NH3 +), 6.12, 5.05(2xm, H19, H20, H21), 5.56, 5.52(2xd, 1H, H14, J=8.3Hz), 4.92(ddd, 1H, H2, J=5.1, 3.8, 4, 8.0Hz), 4.7, 4.69(2xd, 1H, 11-OH, J=6.1Hz),
    #4.06(m, 1H, H□-valyl), 4.3, 4.25, 3.91, 3.88, 2.6-3.6(m, 4xCH2N, SCH, H11, H22), 1.39, 1.06(2xs, (CH3)15, (CH3)18), 0.99, 0.9, 0.84, 0.64(4xd, (CH3)2CH, (CH3)16, (CH3)17, J=6.8Hz)
    Figure US20050215637A1-20050929-C00055
         		Exmaple 13 14-O-[((Piperidin-3(S)-yl)methyl-sulfanyl)-acetyl]mutilin in the form of a hydrochloride (d6-DMSO, 350K): 6.15, 5.05(2xm, H19, H20, H21), 5.55(d, 1H, 5.2Hz, H14), 3.4(d, 1H, H11, J=5.2Hz), 3.05, 2.95, 2.52, 2.31, 2.09, (5xm, 4H, CH2NCH2), 3.2(m, 2H, SCH2C═O), 2.48(m, 2H, CHCH2S), 1.18, 1.45(2xs, (CH3)15,
    #(CH3)18), 0.75, 0.88, (2xd, (CH3)16, (CH3)17, J=5.4Hz)
    Figure US20050215637A1-20050929-C00056
         		Example 14 14-O-[((Azepan-4-(R/S)-yl)-sulfanyl acetyl)]-mutilin in the form of a hydrochloride 400 mg of 14-O-[((N-BOC-azepan-4-(R/S)-yl)-sulfanyl acetyl)-mu- tilin(d6-DMSO): 8.2-8.5(b, 2H, NH2 +), 6.15, 5.1(2xm, H19, H20, H21), 5.52(d, 1H, J=5, 2Hz, H14), 4.52(d, 1H, OH, J=6.2Hz)3.4(t, 1H, H11, J=6.2Hz), 3.3(m, 2H, H22),
    #2.9-3.2(2xm, 3H, SCH, CHNCH), 1.18, 1.45(2xs, (CH3)15, (CH3)18), 0.75, 0.88(2xd, (CH3)16, (CH3)17, J=5.4Hz)
    Figure US20050215637A1-20050929-C00057
         		Example 15 14-O-[((N-(R)-Valyl-azepan-4-(R/S)-yl)-sulfanyl acetyl)]-mutilin-hydro- chloride (d6-DMSO): 7.7-8.0(b, 3H, NH3 +), 6.15, 5.1(2xm, H19, H20, H21), 5.52(d, 1H, J=5, 2Hz, H14), 4.52(d, 1H, OH, J=6.2Hz), 3.4(t, 1H, H11, J=6.2Hz), 4.1(m, α-H-valine), 3.4, 2.6(2xm, 4H, CH2NCH2)3.3(m, 2H, H22),
    #2.9(m, 1H, SCH), 1.18, 1.45(2xs, (CH3)15, (CH3)18), 0.75, 0.88(2xd, (CH3)16, (CH3)17, J=5.4Hz)
    Figure US20050215637A1-20050929-C00058
         		Example 16 14-O-[(N-(R)-Valyl-pyrrolidin-3(S)-yl)-sulfanyl-acetyl]-mutilin-hy- drochloride (CD3OD): Rotamer, 8.1(b, 3H, NH3), 6.3-6.4(m, 1H, H19), 5.75(d, 1H, H14), 5.15(m, 2H, H20), 4.15(m, 1H, NCHCO), 3.9(m, 1H, NCH), 3.6(m, 1H, NCH), 3.42(d, 1H, H11), 3.28-3.35(m, 2H, SCH2CO), 0.95, 0.98(2xd, 6H, (CH3)2CH), 1.08, 1.36(2xs, 6H, (CH3)18,
    #(CH3)15), 0.65, 0.83(2xd, 6H, (CH3)16, (CH3)17)
    Figure US20050215637A1-20050929-C00059
         		Example 17 14-O-[((N-(R)-Pipercolyl-piperidin-4-yl)methyl-sulfanyl)-acetyl]-mu- tilin in the form of a hydrochloride 1H-NMR(d6-DMSO, 350K): 6.15, 5.05(2xm, H19, H20, H21), 5.55(d, 1H, 5, 2Hz, H14), 3.35(d, 1H, H11, J=5.2Hz), 4.3(m, α-H-pipecol), 4.2, 4.05, 3.75(4xm, CH2NCH2), AB-system: 3.12, 3.18, J=14.7Hz, H22),),
    #2.8(m, 1H, SCH), 1.18, 1.45(2xs, (CH3)15, (CH3)18), 0.75, 0.88(2xd, (CH3)16, (CH3)17, J=5.4Hz)
  • In another aspect the present invention provides a compound of formula
    Figure US20050215637A1-20050929-C00060
    wherein
      • the dotted line is a bond (double bond between positions a=b), R1A is hydrogen and R2A is not present, or
      • the dotted line is no bond (single bond between positions a-b) and R1A and R2A independently of each other are hydrogen, halogen or deuterium,
    • R3A is (C1-6)alkyl,
    • R4A is hydrogen, (C1-6)alkyl, a group —C(═NH)—NH2, or the residue of an amino acid,
    • R5A is hydrogen, or
    • R4A and R5A together are a group ═CH—NH2,
    • R6A is hydrogen or deuterium, and
    • mA is 0, 1, 2, 3, 4, or 5.
  • In a compound of formula IA preferably
      • the dotted line is no bond;
      • R1A is hydrogen,
      • R2A is hydrogen,
      • R3A is (C1-4)alkyl, such as methyl,
      • R4A and R5A are as defined above,
      • R6A is hydrogen, and
      • mA is 2, 3 or 4;
        e.g. including pleuromutilins, which are selected from the group consisting of compounds of formula
        Figure US20050215637A1-20050929-C00061
  • wherein REX is as set out in TABLE 2. 1H-NMR-data of the compounds described and obtained according, e.g. analogously, to a process as described in the examples are also indicated in TABLE 2:
    TABLE 2
    Figure US20050215637A1-20050929-C00062
         		Example 18
    Figure US20050215637A1-20050929-C00063
         		Example 18a 14-O-[(3-(R*)-Amino-1-methyl-cyclopentan-1-(R*)-yl)-sulfanyl)-ace- tyl]-mutilin and 14-O-[(3-(S*)-amino-1-methyl-cyclopentan-1-(S*)-yl)-sul- fanyl)-acetyl]-mutilin in the form of a hydrochloride(mixture of trans-diastereoisomers) (DMSO-d6): 7.98(bs, 3H, NH3 +), 6.13, 5.06, 5.03(3xm, H19, H20, H21), 5.55(d, 1H, H14, J=8.2Hz),
    #3.56(m, 1H, H-3′), 3.3—3.3(m, H11, H22), 1.36, 1.30, 1.29, 1.05(4xs, 9H, CH3CS, (CH3)15, (CH3)18), 0.8, 0.62(2xd, (CH3)16, (CH3)17, J=6.9Hz)
    Figure US20050215637A1-20050929-C00064
         		Example 18b 14-O-[(3-(R*)-Amino-1-methyl-cyclopentan-1-(S*)-yl)-suflanyl)-ace- tyl]-mutilin and 14-O-[(3-(S*)-Amino-1-methyl-cyclopentan-1-(R*)-yl)-sul- fanyl)-acetyl]-mutilin in the form of a hydrochloride(mixture of cis-diastereoisomers) (DMSO-d6): 8.03(bs, 3H, NH3 +), 6.13, 5.06, 5.03(3xm, H19, H20, H21), 5.53(d, 1H, H14, J=8.0Hz),
    #4.52(bs, 1H-11-OH), 3.51(m, 1H, H-3′), 3.2-3.4(m, H11, H22), 1.41, 1.40, 1.35, 1.05(4xs, 9H, CH3CS(CH3)15, (CH3)18), 0.8, 0.62(2xd, (CH3)16, (CH3)17, J=6.9Hz)
    Figure US20050215637A1-20050929-C00065
         		Example 19
    Figure US20050215637A1-20050929-C00066
         		Example 19a 14-O-{[(1S*,3S*)-3-((R)-2-Amino-3-methyl-butyrylamino)-1-meth- yl-cyclopentylsulfanyl]-acetyl}-mutilin in the form of a hydrochloride Rotamer 1: (DMSO-d6): 8.48(d, 1H, NH, J=7.1Hz), 8.1(bs, 3H, NH3 +), 6.11, 5.06, 5.03(3xm, H19, H20, H21), 5.55(d, 1H, H14, J=8.5Hz), 4.15(m, 1H, H□-valyl), 3.1-3.5(m, H-1′,
    #H-3′, H11, H22), 1.35, 1.32, 1.05(3xs, CH3CS, (CH3)15, (CH3)18), 0.91, 0.88(d, (CH3)2CH, J=6.8Hz), 0.8, 0.62(2xd, (CH3)16, (CH3)17, J=6.8Hz). Rotamer 2: (DMSO-d6): 8.48(d, 1H, NH, J=7.1Hz), 8.1(bs, 3H, NH3 +), 6.11, 5.06, 5.03(3xm, H19, H20, H21), 5.55(d, 1H, H14,
    #J=8.5Hz), 4.5(bs, 1H, 11-OH), 4.15(m, 1H, □-valyl), 3.1-3.5(m, H-1′, H-3′, H11, H22), 1.35, 1.32, 1.05(3xs, CH3CS, (CH3)15, (CH3)18), 0.91, 0.88(d, (CH3)2CH, J=6.8Hz), 0.8, 0.62(2xd, (CH3)16, (CH3)17, J=6.8Hz)
    Figure US20050215637A1-20050929-C00067
         		Example 19b 14-O-{[(1R*,3R*)-3-((R)-2-Amino-3-methyl-butyrylamino)-1-meth- yl-cyclopentylsulfanyl]-acetyl}-mutilin-hydrochloride (DMSO-d6): Rotamers: 8.53(d, 1H, NH, J=7.2Hz), 8.1(bs, 3H, NH3 +), 6.12, 5.06, 5.03(3xm, H19, H20, H21), 5.55(d, 1H, H14, J=8.4Hz), 4.52(d, 1H, 11-OH, J=6.1Hz), 4.25(m, 1H, H□-valyl), 3.2-3.5(m,
    #H-1′, H-3′, H11, H22), 1.42, 1.39, 1.35, 1.05(4xs, 9H, CH3CS, (CH3)15, (CH3)18), 0.91, 0.88(d, (CH3)2CH, J=6.8Hz), 0.8, 0.62(2xd, (CH3)16, (CH3)17, J=6.8Hz)
    Figure US20050215637A1-20050929-C00068
         		Example 19c 14-O-{[(1R*,3S*)-3-((R)-2-Amino-3-methyl-butylrylamino)-1-meth- yl-cyclopentylsulfanyl]-acetyl}-mutilin and 14-O-{[(1S*,3R*)-3-((R)-2-Amino-3-methyl-butyrylamino)-1-meth- yl-cyclopentylsulfanyl]-acetyl}-mutilin in the form of a hydrochloride(mixture of cis-diastereoisomers) (DMSO-d6): 8.4, 8.3(2xd, 1H, NH, J=7.2Hz), 8.17(bs, 3H, NH3 +),
    #6.12, 5.06, 5.02(3xm, H19, H20, H21), 5.55(d, 1H, H14, J=8.3Hz), 4.53(bs, 1H, 11-OH), 4.15(m, 1H, H□-valyl), 3.2-3.5(m, H-1′, H-3′, H11, H22), 1.36, 1.35, 1.32, 1.05(4xs, 9H, CH3CS, (CH3)15, (CH3)18), 0.92, 0.91, 0.89, 0.88(2xd, 6H, (CH3)2CH, J=6.8Hz), 0.8, 0.62(2xd, (CH3)16, (CH3)17,
    #J=6.8Hz)
    Figure US20050215637A1-20050929-C00069
         		Example 20 14-O-[((3-(R/S)-Amino-cyclohexan-1-(R/S)-methyl-1-yl)sulfanyl)ace- tyl]mutilin in the form of a hydrochloride (d6-DMSO): Rotamers: 7.90(b, 3H, NH3 +), 6.15, 5.1(2xm, H19, H20, H21), 5.52(d, 1H, J=5, 2Hz, H14), 4.5(2xd, 1H, OH, J=6Hz), 3.4(t, 1H, H11, J=6Hz), 3.3(m, 2H, H22), 3.1(m, 1H, NCH), 1.2,
    #1.25(2xs, 3H, CH3CS)-1.18, 1.45(2xs, (CH3)15, (CH3)18), 0.9(m, 6H, CH(CH3)2), 0.75, 0.88(2xd, (CH3)16, (CH3)17, J=5.4Hz)
    Figure US20050215637A1-20050929-C00070
         		Example 21a 14-O-[(3-(R*)-Guanidino-cyclohexan-1-(R*)-methyl-1-yl)-ace- tyl]mutilin in the form of a hydrochloride + 14-O-[(3-(S*)-Guani- dino-cyclohexan-1-(S*)-methyl-1-yl)-acetyl]mutilin in the form of a hydrochloride (d6-DMSO): 10.7, 8.6, 7.65, (3xm, 2H, NH), 6.7-7.5(b, 2HNH), 6.15, 5.1(2xm, H19, H20, H21), 5.52(d, 1H, J=5, 2Hz, H14),
    #5.5(d, 1H, OH, J=6Hz), 3.9, 3.6(2x, m1H, CHNH), 3.4(t, 1H, H11, J=6Hz), 3.3(m, 2H, H22), 1.4, 1.45(2xs, 3H, CH3CS), 1.18, 1.45(2xs, (CH3)15, (CH3)18), 0.75, 0.88(2xd, (CH3)16, (CH3)17, J=5.4Hz). Example 21b 14-O-[(3-(R*)-Guanidino-cyclohexan-1-(S*)-meth- yl-1-yl)-acetyl]mutilin in the form of a hydrochloride +
    #14-O-[(3-(S*)-Guan- idino-cyclohexan-1-(R*)-methyl-acetyl]mu- tilin in the form of a hydrochlorde (d6-DMSO): 10.7, 8.6, 7.65, (3xm, 2H, NH), 6.7-7.5(b, 2HNH), 6.15, 5.1(2xm, H19, H20, H21), 5.52(d, 1H, J=5, 2Hz, H14), 5.5(d, 1H, OH, J=6Hz), 3.9, 3.6(2x, m1H, CHNH), 3.4(t, 1H, H11, J=6Hz), 3.3(m, 2H, H22), 1.4, 1.45(2xs, 3H, CH3CS), 1.18,
    #1.45(2xs, (CH3)15, (CH3)18), 0.75, 0.88(2xd, (CH3)16, (CH3)17, J=5.4Hz)
    Figure US20050215637A1-20050929-C00071
         		Example 22a 14-O-[(3-(R*)-(R)-Valylamino-1-(R*)-methyl-1-yl)sulfanyl)-ace- tyl]mutilin and 14-O-[(3-(S*)-(R)-Valylamino-1-(S*)-methyl-1-yl)sul- fanyl)-acetyl]mutilin in the form of a hydrochloride (diastereoisomeric mixture) (d6-DMSO): 8.3(m, 1H, NHC═O), 8.1(b, 3H, NH3 +), 6.15, 5.1(2xm, H19, H20, H21), 5.52(d, 1H, J=5, 2Hz, H14), 3.9(m,
    #1H, a-H-valyl), 3.3-3.1(4xm, 4H, H11, H22, CHNH3 +), 4.5(b, 1H, OH), 1.25(b, 3H, CH3CS), 1.18, 1.45(2xs, (CH3)15, (CH3)18), 0.9(m, 6H, CH(CH3)2), 0.75, 0.88(2xd, (CH3)16, (CH3)17, J=5.4Hz) Example 22b 14-O-[(3-(R*)-(R)-Valylamino-1-(S*)-methyl-1-yl)sulfanyl)-ace- tyl]mutilin and 14-O-[(3-(S*)-(R)-Valyl
    #amino-1-(R*)-methyl-1-yl)sulfa- nyl)-acetyl]mutilin in the form of a hydrochloride (diastereoisomeric mixture) (d6-DMSO): 8.35(m, 1H, NHC═O), 8.1(b, 3H, NH3 +), 6.15, 5.1(2xm, H19, H20, H21), 5.52(d, 1H, J=5, 2Hz, H14), 3.95(m, 1H, a-H-valyl), 3.75(m, 1H, CHNH), 3.2-3.5(3xm, 3H, H11, H22,), 4.5(b, 1H, OH), 1.25(b, 3H, CH3CS), 1.18,
    #1.45(2xs, (CH3)15, (CH3)18), 0.9(m, 6H), CH(CH3)2), 0.75, 0.88(2xd, (CH3)16, (CH3)17, J=5.4Hz)
    Figure US20050215637A1-20050929-C00072
         		Example 23 14-O-[(3-(R/S)-Dimethylamino-methylenimino-cyclohexan-1-(R/S)-meth- yl-1-yl)-acetyl]mutilin in the form of a hydrochloride (d6-DMSO): 9.2, 8.1(2xb, 2H, NH), 6.15, 5.1(2xm, H19, H20, H21), 5.52(d, 1H, J=5, 2Hz, H14), 4.5(d, 1H, OH, J=6Hz), 3.7(m, 1H, CHNH), 3.4(t, 1H, H11, J=6Hz), 3.3(m, 2H, H22), 3.1(b, 6H,
    #N(CH3)2), 1.4, 1.45(2xs, 3H, CH3CS), 1.18, 1.45(2xs, (CH3)15, (CH3)18), 0.75, 0.88(2xd, (CH3)16, (CH3)17, J=5.4Hz)
  • In another aspect the present invention provides 14-O-(oximino-(C3-8)cycloalkyl-sulfanylmethylcarbonyl)-pleuromutilins and 14-O-(hydrazono-(C3-8)cycloalkyl-sulfanylmethylcarbonyl)-pleuromutlins, such as a compound of formula
    Figure US20050215637A1-20050929-C00073
    wherein R1B has the meaning of R1A as defined above,
    • R2B has the meaning of R2A as defined above,
    • R10B has the meaning of R6A as defined above,
    • the dotted line has the meaning as defined above,
    • mB has the meaning of ma as defined above,
    • R3B is hydrogen or (C1-6)alkyl,
    • XB is —O—R4B or —NR5BR6B,
    • R4B is hydrogen or (C1-6)alkyl, optionally substituted by a group —NR7BR8B,
    • R5B and R6B independently of each other are (C1-4)alkyl,
    • R7B and R8B independently of each other are (C1-4)alkyl, or
    • R7B and R8B together with the nitrogen atom to which they are attached form aliphatic heterocyclyl, having 5 to 8 ring members, and
    • R9B is hydrogen or (C1-4)alkyl.
  • In a compound of formula IB preferably
      • R1B has is hydrogen,
      • R2B has is hydrogen,
      • the dotted line is not present (single bond),
      • mB is 2, 3 or 4,
    • R3B is hydrogen or (C1-4)alkyl, such as methyl,
      • XB is as defined above,
      • R4B is hydrogen or (C1-4)alkyl, such as ethyl, substituted by a group —NR7BR8B,
      • R5B and R6B are as defined above,
      • R7B and R8B independently of each other are (C1-4)alkyl, e.g. ethyl, or R7B and R8B together with the nitrogen atom to which they are attached form pyrrolidine or piperidine,
      • R9B is as defined above, and
      • R10B is hydrogen;
        e.g. including 14-O-(oximino-(C1-3)cycloalkyl-sulfanylmethylcarbonyl)-pleuromutilins and 14-O-(hydrazono-(C3-8)cycloalkyl-sulfanylmethylcarbonyl)-pleuromutilins, which are selected from the group consisting of of compounds of formula
        Figure US20050215637A1-20050929-C00074
  • wherein REX is as set out in TABLE 3. 1H-NMR-data of the compounds described and obtained according, e.g. analogously, to a process as described in the examples, are also indicated in TABLE 3:
    TABLE 3
    Figure US20050215637A1-20050929-C00075
         		Example 24 14-O-{[(3-Hydroxyimino-cyclopentan-(R/S)-yl)-sulfanyl]-ace- tyl}-mutilin(syn and anti forms) Syn*-form: (DMSO-d6): 10.33(s, 1H,═NOH), 6.15, 5.07, 5.03(3xm, H19, H20, H21), 5.55(d, 1H, H14, J=8.3Hz), 4.5(d, 1H, 11-OH, J=6.1Hz), 3.25-3.45(m, SCH, H11, H22), 2.67(m, 1H, H-2a′), 1.35, 1.05(2xs, (CH3)15,
    #(CH3)18), 0.8, 0.62(2xd, (CH3)16, (CH3)17, J=7Hz). Anti*-form: (DMSO-d6): 10.36(s, 1H,═NOH), 6.15, 5.07, 5.02(3xm, H19, H20, H21), 5.55(d, 1H, H14, J=8.3Hz), 4.5(d, 1H, 11-OH, J=6.1Hz), 3.25-3.45(m, SCH, H11, H22), 2.74(m, 1H, H-2a′)1.35, 1.05(2xs, (CH3)15, (CH3)18), 0.8, 0.62(2xd,
    #(CH3)16, (CH3)17, J=6.8Hz)
    Figure US20050215637A1-20050929-C00076
         		Example 25a 14-O-{[(3-(E/Z)-Hydroxyimino-1-methyl-cyclopentan-(R*)-yl)-sul- fanyl]-acetyl}mutilin 1HNMR(DMSO-d6): 10.33, 10.28(2xs, 1H,═NOH), 6.15, 5.07, 5.02(3xm, H19, H20, H21), 5.55, 5.53(2xd, 1H, H14, J=8.3Hz), 4.5, 4.48(2xd, 1H, 11-OH, J=6.0Hz), 3.20-3.45(m, SCH, H11, H22), 1.35, 1.05(3xs, CH3CS,
    #(CH3)15, (CH3)18), 0.8, 0.62(2xd, (CH3)16, (CH3)17, J=6.9Hz) Example 25b 14-O-{[(3-(E/Z)-Hydroxyimino-1-methyl-cyclopentan-(S*)-yl)-sul- fanyl]-acetyl}-mutilin (DMSO-d6): 10.32, 10.27(2xs, 1H,═NOH), 6.15, 5.07, 5.02(3xm, H19, H20, H21), 5.55(d, 1H, H14, J=8.3Hz), 4.5(d, 1H, 11-OH, J=6.1Hz),
    #3.20-3.45(m, SCH, H11, H22), 1.35, 1.05(3xs, CH3CS, (CH3)15, (CH3)18), 0.8, 0.62(2xd, (CH3)16, (CH3)17, J=6.8Hz)
    Figure US20050215637A1-20050929-C00077
         		Example 26 14-O-{[(3-(2-Diethylamino-ethoxyimino)-cyclopentan-(R/S)-yl)-sul- fanyl]-acetyl}-mutilin in the form of a hydrochloride (syn/anti mixture) (DMSO-d6): 9.9(bs, 1H, NH+), 6.15, 5.07, 5.03(3xm, H19, H20, H21), 5.55(d, 1H, H14, J=8.1Hz), 4.3(m, 2H, OCH2), 3.1-3.4(m, NCH2, H11, H22), 1.35, 1.05(2xs,
    #(CH3)15, (CH3)18), 0.8, 0.62(2xd, (CH3)16, (CH3)17, J=6.8Hz)
    Figure US20050215637A1-20050929-C00078
         		Example 27a 14-O-[((E*-Hydroximino-cyclohexan-3-(R*)-yl)-sulfanyl)-ace- tyl]mutilin (d6-DMSO): 10.31(s, 1H, HON═C), 6.15, 5.1(2xm, H19, H20, H21), 5.52(d, 1H, J=5, 2Hz, H14), 4.55(d, 1H, OH, J=5Hz)3.4(t, 1H, H11, J=5Hz), 3.3(m, 2H, H22), 2.95(m, 1H, SCH), 2.78, 1.95(2xm, 2H, CH2C═N), 2.57, 2.09(2xm, 2H,
    #CH2═C═N), 1.18, 1.45(2xs, (CH3)15, (CH3)18), 0.75, 0.88(2xd, (CH3)16, (CH3)17, J=5.4Hz). [α]D = 15.87° (c = 1, MeOH) Example 27b 14-O-[((E*-Hydroximino-cyclohexan-3-(S*)-yl)-sulfanyl)-ace- tyl]mutilin (d6-DMSO): 10.31(s, 1H, HON═C), 6.15, 5.1(2xm, H19, H20, H21), 5.52(d, 1H, J=5,
    #2Hz), H14), 4.50(d, 1H, OH, J=5Hz)3.4(t, 1H, H11, J=5Hz), 3.3(m, 2H, H22, 2.95(m, 1H, SCH), 2.78, 1.95(2xm, 2H, CH2C═N), 2.57, 2.09(2xm, 2H, CH2═C═N), 1.18, 1.45(2xs, (CH3)15, (CH3)18), 0.75, 0.88(2xd, (CH3)16, (CH3)17, J=5.4Hz). [α]D = 38.5° c = 1, MeOH) Example 27c 14-O-[((Z*-Hydroximino-cyclo
    #hexan-3-(R*)-yl)-sul- fanyl)-acetyl]mutilin (d6-DMSO): 10.31(s, 1H, HON═C), 6.15, 5.1(2xm, H19, H20, H21), 5.52(d, 1H, J=5, 2Hz, H14), 4.50(d, 1H, OH, J=5Hz), 3.45(t, 1H, H11, J=5Hz), 3.3(m, 2H, H22), 2.90(m, 1H, SCH), 3.05, 2.05(2xm, 2H, CH2C═N), 2.2(m, 2H, CH2═C═N), 1.18, 1.45(2xs, (CH3)15, (CH3)18), 0.75, 0.88(2xd,
    #(CH3)16, (CH3)17, J=5.4Hz). [α]D = 13.62° (c = 1, MeOH) Example 27d 14-O-[((Z*-Hydroximino-cyclohexan-3-(S*)-yl)-sul- fanyl)acetyl]mutilin (d6-DMSO): 10.31(s, 1H, HON═C), 6.15, 5.1(2xm, H19, H20, H21), 5.52(d, 1H, J=5, 2Hz, H14), 4.50(d, 1H, OH, J=5Hz), 3.45(t, 1H, H11, J=5Hz), 3.3(m, 2H, H22),
    #2.90(m, 1H, SCH), 3.05, 2.05(2xm, 2H, CH2C═N), 2.2(m, 2H, CH2═C═N), 1.18, 1.45(2xs, (CH3)15, (CH3)18), 0.75, 0.88(2xd, (CH3)16, (CH3)17, J=5.4Hz). [α]D = 42.83° (c = 1, MeOH)
    Figure US20050215637A1-20050929-C00079
         		Example 28a 14-O-[((E-Hydroximino-cyclohexan-3-(R/S)-methyl-1-yl)-sul- fanyl)acetyl]mutilin (d6-DMSO): 10.31(s, 1H, HON═C), 6.15, 5.1(2xm, H19, H20, H21), 5.52(d, 1H, J=5, 2Hz, H14), 4.50(d, 1H, OH, J=5Hz), 3.45(t, 1H, H11, J=5Hz), 3.25(m, 2H, H22), 1.25(s, 3H, CH3, CH3CS)1.18, 1.45(2xs, (CH3)15,
    #(CH3)18), 0.75, 0.88(2xd, (CH3)18, (CH3)17, J=5.4Hz) Example 28b 14-O-[((Z-Hydroximino-cyclohexan-3-(R/S)-methyl-1-yl)-sul- fanyl)acetyl]mutilin (d6-DMSO): 10.31(s, 1H, HON═C), 6.15, 5.1(2xm, H19, H20, H21), 5.52(d, 1H, J=5, 2Hz, H14), 4.50(d, 1H, OH, J=5Hz), 3.45(t, 1H, H11, J=5Hz), 3.25(m, 2H, H22), 2.7(d,
    #CHC═N, J=12Hz), 1.25(s, 3H, CH3, CH3CS)1.18, 1.45(2xs, (CH3)15, (CH3)18), 0.75, 0.88(2xd, (CH3)16, (CH3)17, J=5.4Hz)
    Figure US20050215637A1-20050929-C00080
         		Example 29 14-O-[((Z/E_Hydroximino-cyclohexan-3-(R/S)-methyl-1-yl(R/S)-meth- yl-1-yl)-sulfanyl)acetyl]mutilin (d6-DMSO): Diastereoisomers: 10.2, 10.28(3xs, 1H, HON═C), 6.15, 5.1(2xm, H19, H20, H21), 5.52(d, 1H, J=5, 2Hz, H14), 4.50(d, 1H, OH, J=5Hz), 3.45(t, 1H, H11, J=5Hz), 3.25(m, 2H, H22), 3.25, 1.7(2xm, 2H, CH2C═N), 1.3,
    #1.34(2xs, 3H, CH3, CH3CS), 1.18, 1.45(2xs, (CH3)15, (CH3)18), 0.75, 0.88(2xd, (CH3)16, (CH3)17, J=5.4Hz). MS-ESI: 534(M + 1)
    Figure US20050215637A1-20050929-C00081
         		Example 30 14-O-{[(3-(2-Diethylamino-ethoxyimino)-cyclohexan-(R/S)-yl)-sul- fanyl]-acetyl}-mutilin hydrochloride(E/Z mixture) (DMSO-d6): 9.7(bs, 1H, NH+), 6.15, 5.07, 5.03(3xm, H19, H20, H21), 5.55(d, 1H, H14, J=8.0Hz), 4.5, 4.25(2xm, 2H, OCH2), 3.0-3.45(m, NCH2, H11, H22), 1.35, 1.05(2xs, (CH3)15,
    #(CH3)18), 0.8, 0.62(2xd, (CH3)16, (CH3)17, J=6.8Hz)
    Figure US20050215637A1-20050929-C00082
         		Example 31 14-O-[(((E/Z)-Dimethylaminimino-cyclohexan-3-(R/S)-1-yl)-sul- fanyl)acetyl]mutilin (d6-DMSO): Diastereoisomers: 6.15, 5.1(2xm, H19, H20, H21), 5.52(d, 1H, J=5, 2Hz, H14), 4.50(d, 1H, OH, J=5Hz), 3.45(t, 1H, H11, J=5Hz), 3.3(m, 2H, H22), 3.2, 2.8(2xm, 1H, CHS), 2.95, 1.85(2xm, 2H, CH2C═N), 1.18, 1.45(2xs,
    #(CH3)15, (CH3)18), 0.75, 0.88(2xd, (CH3)16, (CH3)17, J=5.4Hz)
    Figure US20050215637A1-20050929-C00083
         		Example 32a 14-O-[((E*-Hydroximino-cycloheptan-3-(R/S)-yl)-sulfanyl)-ace- tyl]mutilin NMR(d6-DMSO): 10.35(s, 1H, HON═C), 6.15, 5.1(2xm, H19, H20, H21), 5.52(d, 1H, J=5, 2Hz, H14), 4.5(d, 1H, OH, J=6.1Hz), 3.4(t, 1H, H11, J=6.1Hz)), 3.3(m, 2H, H22), 1.18, 1.45(2xs, (CH3)15, (CH3)18), 0.75, 0.88(2xd, (CH3)16,
    #(CH3)17, J=5.4Hz). Example 32b 14-O-[((Z*-Hydroximino-cycloheptan-3-(R/S)-yl)-sul- fanyl)acetyl]mutilin (d6-DMSO): 10.35(s, 1H, HON═C), 6.15, 5.1(2xm, H19, H20, H21), 5.52(d, 1H, J=5, 2Hz, H14(, 4.5(d, 1H, OH, J=5Hz)3.4)t, 1H, H11, J=5Hz), 3.3(m, 2H, H22), 3.05(m, 1H, SCH), 1.18, 1.45(2xs, (CH3)15, (CH3)18),
    #0.75, 0.88(2xd, (CH3)16, (CH3)17, J=5.4Hz)
    Figure US20050215637A1-20050929-C00084
         		Example 33 14-O-{[(3-(2-Diethylamino-ethoxyimino)-cyclohept-(R/S)-yl)-sul- fanyl]-acetyl}-mutilin hydrochloride(E/Z mixture) (DMSO-d6): 9.85(bs, 1H, NH+), 6.15, 5.06, 5.03(3xm, H19, H20, H21), 5.55(d, 1H, H14, J=8.5Hz), 4.52, 4.53(2xd, 1H, 11-OH, J=6.2Hz), 4.28(m, 2H, OCH2), 3.0-3.45(m, NCH2, H11, H22),
    #1.35, 1.05(2xs, (CH3)15, (CH3)18), 0.8, 0.62(2xd, (CH3)16, (CH3)17, J=6.8Hz)
    Figure US20050215637A1-20050929-C00085
         		Example 34 14-O-{[(3-(2-Pyrrolinine-1-yl-ethoxyimino)-cyclohept-1-(R/S)-yl)-sul- fanyl]-acetyl}-mutilin hydrochloride(E/Z mixture) - (DMSO-d6): 10.2(bs, 1H, NH+), 6.15, 5.08, 5.05(3xm, H19, H20, H21), 5.58(d, 1H, H14, J=8.3Hz), 4.53, 4.57(2xd, 1H, 11-OH, J=6.1Hz), 4.26(m, 2H, OCH2), 3.0-3.4NCH2, H11, H22),
    #1.38, 1.08(2xs, (CH3)15, (CH3)18), 0.83, (2xd, (CH3)16, (CH3)17, J=6.8Hz).
    Figure US20050215637A1-20050929-C00086
         		Example 35 14-O-{[(3-(2-Piperidine-1-yl-ethoxyimino)-cyclohept-(R/S)-yl)-sul- fanyl]-acetyl}-mutilin in the form of a hydrochloride(E/Z mixture) 1HNMR(DMSO-d6): 10.0(bs, 1H, NH+), 6.15, 5.06, 5.03(3xm, H19, H20, H21), 5.55(d, 1H, H14, J=8.4Hz), 4.5, 4.55(2xd, 1H, 11-OH, J=6.2Hz), 4.32(m, 2H, OCH2), 2.8-3.5(m, NCH2,
    #H11, H22), 1.35, 1.05(2xs, (CH3)15, (CH3)18), 0.8, 0.62(2xd, (CH3)16, (CH3)17, J=6.8Hz)
  • Figure US20050215637A1-20050929-C00087
    wherein
    • R1c has the meaning of R1A as defined above,
    • R2c has the meaning of R2A as defined above,
    • the dotted line has the meaning as defined above,
    • R4c has the meaning of R6A as defined above, and
    • R3c is amino, (C1-4)alkylamino, di(C1-4)alkylamino, the residue of an amino acid, hydroxy, or (C1-4)alkoxy.
  • In a compound of formula Ic preferably
      • R1c is hydrogen,
      • R2c is hydrogen,
      • R4c is hydrogen,
      • the dotted line is not present (single bond), and
      • R3c is amino, di(C1-4)alkylamino, or the residue of an amino acid;
        e.g. including pleuromutilins which are selected from the group consisting of of compounds of formula
        Figure US20050215637A1-20050929-C00088
  • wherein REX is as set out in TABLE 4. 1H-NMR-data of the compounds described and obtained according, e.g. analogously, to a process as described in the examples are also indicated in TABLE 4:
    TABLE 4
    Figure US20050215637A1-20050929-C00089
         		Example 36a 14-O-{[(3S,3aS,6S,6aR)-6-Amino-hexahydro-furo[3,2-b]furan-3-yl- sulfanyl]-acetyl}-mutilin in the form of a hydrochloride - (DMSO-d6): 8.3(bs, 3H, NH3 +), 6.15, 5.05, 5.02(3xm, H19, H20, H21), 5.55(d, 1H, 8.2Hz, H14), 4.65, 4.55(2xm, H11-OH, H-3a′, H6a′), 3.6-4.1(m, 5H, H-2′, H-5′, H-6′), 3.3-3.5(m, 4H,
    #H11, H-3′, H22), 1.35, 1.05(2xs, (CH3)15, (CH3)18), 0.8, 0.62(2xd, (CH3)16, (CH3)17, J=6.5Hz) Example 36b 14-O-{[(3R,3aS,6S,6aR)-6-Amino-hexahydro-furo[3,2-b]furan-3-yl- sulfanyl]-acetyl}-mutilin-hydrochloride (DMSO-d6): 8.3(bs, 3H, NH3 +), 6.15, 5.07, 5.03(3xm, H19, H20, H21),
    #5.55(d, 1H, H14, J=8.2Hz), 4.60, 4.7(2xm, H-3a′, H6a′), 3.6-4.1(m, 5H, H-2′, H-5′, H-6′), 3.3-3.5(m, 4H, H11, H-3′, H22), 1.35, 1.05, (2xs, (CH3)15, (CH3)18), 0.8, 0.62(2xd, (CH3)16, (CH3)17, J=6.9Hz) Example 36c 14-O-{[(3S,3aS,6R,6aR)-6-Amino-hexahydro-furo[3,2-b]furan-3-yl- sulfanyl]-ace
    #tyl}-mutilin-hydrochloride (DMSO-d6): 8.25(bs, 3H, NH3 +), 6.15, 5.06, 5.03(3xm, H19, H20, H21), 5.55(d, 1H, H14, J=8.4Hz), 4.60(m, 2H, H-3a′, H6a′), 3.55-4.2(m, 5H, H-2′, H-5′, H-6′), 3.35-3.5(m, 4H, H11, H-3′, H22), 1.35, 1.05(2xs, (CH3)15, (CH3)18), 0.8, 0.62(2xd, (CH3)16, (CH3)17, J=6.9Hz)
    Figure US20050215637A1-20050929-C00090
         		Example 37 14-O-{[(3S,3aS,6S,6aR)-6-Dimethylamino-hexahydro-furo[3,2-b]fur- an-3-ylsulfanyl]-acetyl}-mutilin in the form of a hydrochloride (DMSO-d6): 11.25(bs, 1H, NH+), 6.15, 5.06, 5.03(3xm, H19, H20, H21), 5.55(d, 1H, H14, J=8.2Hz), 4.60(m, 1H, H3a′), 3.3-4.1(m, H-2′, H-5′, H-6′, H-6a′, H11, H-3′, H22),
    #2.8, 2.7(2xd, 6H, NH+(CH3)2, J=4.5Hz), 1.35, 1.05(2xs, (CH3)15, (CH3)18), 0.8, 0.62(2xd, (CH3)16, (CH3)17, J=6.9Hz)
    Figure US20050215637A1-20050929-C00091
         		Example 38a 14-O-{[(3S,3aS,6S,6aR)-6-((R)-Amino-3-methyl-butyrylamino)-hexa- hydro-furo[3,2-b]fur- an-3-ylsulfanyl]-acetyl}-mutilin in the form of a hydrochloride (DMSO-d6): 8.65(d, 1H, NH, J=8.2Hz), 7.8(bs, 3H, NH3 +), 6.15, 5.07, 5.02(3xm, H19, H20, H21), 5.55(d, 1H, 5, 2Hz, H14), 4.55, 4.45(2xm, 3H, H11—OH,
    #H-3a′, H6a′), 3.6-4.2(m, 5H, H-2′, H-5′, H-6′), 3.4-3.5(m, 4H, H11, H-3′, H22), 1.35, 1.05(2xs, (CH3)15, (CH3)18), 0.88, 0.92(2xd, (CH3)2CH, J=6.8Hz), 0.8, 0.62(2xd, (CH3)16, (CH3)17, J=6.8Hz) Example 38b 14-O-{[(3R,3aS,6S,6aR)-6-((R)-Amino-3-methyl-butyrylamino)-hexa- hydro-furo[3,2-b]furan-3-ylsulfanyl]-ace
    #tyl}-mutilin in the form of a hydrochloride (DMSO-d6): 8.8(d, 1H, NH, J=7.1Hz), 8.15(bs, 3H, NH3 +), 6.15, 5.07, 5.03(3xm, H19, H20, H21), 5.55(d, 1H, 7.9Hz, H14), 4.6, 4.4(2xm, 2H, H-3a′, H6a′), 3.6-4.2(m, 5H, H-2′, H-5′, H-6′), 3.2-3.5(m, 4H, H11, H-3′, H22), 1.35, 1.05(2xs, (CH3)15, (CH3)18), 0.89, 0.91(d,
    #(CH3)2CH, J=6.8Hz), 0.8, 0.62(2xd, (CH3)16, (CH3)17, J=6.8Hz) Example 38c 14-O-{[(3S,3aS,6R,6aR)-6-((R)-Amino-3-methyl-butyrylamino)-hexa- hydro-furo[3,2-b]furan-3-ylsulfanyl]-acetyl}-mutilin in the form of a hydrochloride (DMSO-d6): 8.4(d, 1H, NH, J=7.8Hz), 8.1(bs, 3H, NH3 +), 6.15, 5.06, 5.02(3xm, H19,
    #H20, H21), 5.55(d, 1H, 8.2Hz, H14), 4.6, 4.5(2xm, 2H, H-3a′, H6a′), 3.6-4.4(m, 5H, H-2′, H-5′, H-6′), 3.3-3.5(m, 4H, H11, H-3′, H22), 1.35, 1.05(2xs, (CH3)15, (CH3)18), 0.94, 0.90(d, (CH3)2CH, J=6.8Hz), 0.8, 0.62(2xd, (CH3)16, (CH3)17, J=6.8Hz)
    Figure US20050215637A1-20050929-C00092
         		Example 39a 14-O-{[(3S,3aS,6R,6aR)-6-Hydroxy-hexahydro-furo[3,2-b]furan-3-yl- sulfanyl]-acetyl}-mutilin (DMSO-d6): 6.15, 5.07, 5.03(3xm, H19, H20, H21), 5.55(d, 1H, H14, J=8.3Hz), 4.85(d, 1H, 6′-OH, J=6.4Hz), 4.5(d, 1H, 11-OH, J=6.1Hz), 3.65-4.45(m, H-3a′, H-6a′, H-2′, H-5′, H-6′), 3.3-3.45(m, H-5′, H11, H-3′,
    #H22), 1.35, 1.05(2xs, (CH3)15, (CH3)18), 0.8, 0.62(2xd, (CH3)16, (CH3)17, J=6.9Hz) Example 39b 14-O-{[(3R,3aS,6R,6aR)-6-Hydroxy-hexahydro-furo[3,2-b]furan-3-yl- sulfanyl]-acetyl}-mutilin (DMSO-d6): 6.15, 5.06, 5.03(3xm, H19, H20, H21), 5.55(d, 1H, H14, J=8.3Hz), 4.8(d, 1H, 6′-OH, J=5.7Hz),
    #4.5(d, 1H, 11-OH, J=6.1Hz), 3.7-4.45(m, H-3a′, H-6a′, H-2′, H-5′, H-6′), 3.2-3.5(m, H-5′, H11, H-3′, H22), 1.35, 1.05(2xs, (CH3)15, (CH3)18), 0.8, 0.62, (2xd, (CH3)16, (CH3)17, J=6.8Hz) Example 39c 14-O-{[(3S,3aS,6S,6aR)-6-Hydroxy-hexahydro-furo[3,2-b]furan-3-yl- sulfanyl]-acetyl}-mu
    #tilin (DMSO-d6): 6.1, 5.07, 5.02(3xm, H19, H20, H21), 5.55(d, 1H, H14, J=8.3Hz), 5.2(d, 1H, 6′-OH), 3.55-4.55(m, 11-OH, H-3a′, H-6a′, H-2′, H-5′, H-6′), 3.3-3.45(m, H11, H-3′, H22), 1.35, 1.05(2xs, (CH3)15, (CH3)18), 0.8, 0.62(2xd, (CH3)16, (CH3)17, J=6.8Hz)
  • In another aspect the present invention provides 14-O-(hydroxy- or oxo)-(heterocyclyl-sulfanylmethylcarbonyl)-pleuromutlins, wherein heterocyclyl is an aliphatic ring of 4 to 8 ring members, preferably 5 to 7, comprising one nitrogen as the heteroarom, e.g. a compound of formula
    Figure US20050215637A1-20050929-C00093
    wherein
    • R1D has the meaning of R1A as defined above,
    • R2D has the meaning of R2A as defined above,
    • R4D has the meaning of R6A as defined above,
    • the dotted line has the meaning as defined above, and
    • R3D is aliphatic heterocyclyl of 4 to 8 ring members, and comprising one nitrogen atom as the heteroatom, or (C4-8)cycloalkyl, which heterocyclyl or cycloalkyl is substituted by hydroxy or oxo.
  • In a compound of formula ID preferably
      • R1D is hydrogen or deuterium,
      • R2D is hydrogen or deuterium,
      • the dotted line is not present (single bond),
      • R3D is as defined above, e.g. heterocyclyl has preferably 5 to 7 ring members, e.g. heterocyclyl is attached to the sulphur in a compound of formula ID via a carbon bond; such as hydroxypyrrolidino, hydroxypiperidino, oxo-perhydroazepinyl; and cycloalkyl is preferably (C5-8)cycloalkyl, e.g. cyclopentanonyl,
      • R4D is hydrogen or deuterium,
        e.g. including 14-O-(hydroxy- or oxo-hetrocyclyl-sulfanylmethylcarbonyl)-pleuromutilins, wherein heterocyclyl is an aliphatic ring of 4 to 8 ring members, preferably 5 to 7, comprising one nitrogen as the heteroarom, which are selected from the group consisting of e.g. including pleuromutilins which are selected from the group consisting of compounds of formulae
        Figure US20050215637A1-20050929-C00094
  • wherein REX is as set out in TABLE 5. The compounds of TABLE 5 are compounds of formula IEX, with the exception of the compound of Example 41 which is a compound of formula I″EX. 1H-NMR-data of the compounds described and obtained according, e.g. analogously, to a process as described in the examples are also indicated in TABLE 5:
    TABLE 5
    Figure US20050215637A1-20050929-C00095
         		Example 40 14-O-[(3-R*-Hydroxypyrrolidine-4-(R*)yl)-sulfanyl-acetyl]-mutilin and 14-O-[(3-S*-Hydroxypyrrolidine-4-(S*)yl)-sulfanyl-acetyl]-mu- tilin in the form of a hydrochloride(diastereoisomeric mixture) (d-6-DMSO): 9.4, 9.65(2xb, 2H, NH2 +), 3.6, 3.2(2xm, 4H, CH2NCH2), 4.45(m, 1H, CHO), 3.45-3.32(m, 3H, H11H22), 3.95(m, 1H, CHS), 1.18,
    #1.45(2xs, (CH3)15, (CH3)16), 0.75, 0.88(2xd, (CH3)16, (CH3)17, J=5.4Hz 0.98)
    Figure US20050215637A1-20050929-C00096
         		Example 41 2,2,4-Trideutero-14-O-[((3-(S*)-hydroxy-piperidine-4-(S*)-yl)sul- fanyl)-acetyl]mutilin in the form of a deuterochloride (d-6-DMSO, 350K): 8.05(b, 3H, NH3 +), 4.25-4.1(m, 3H, CH2N, NHCHC═O), 3.75(m, 1H, CHO), 3.45-3.32(m, 3H, H11H22), 2.89(m, 1H, CHS), 1.18, 1.45(2xs, (CH3)15, (CH3)18), 0.9(m, 6H,
    #CH(CH3)2), 0.75, 0.88(2xd, (CH3)16, (CH3)17, J=5.4Hz 0.98). The signals of the 2,2′-and 4-protons of the tricyclic moiety oare missing in thast spectrum. Mass Spectroscopy(MS): m/e: 496
    Figure US20050215637A1-20050929-C00097
         		Example 42 14-O-[(3R*-Hydroxypiperidin-4-(R*)yl)-sulfanyl-acetyl]mutilin in the form of a hydrochloride + 14-O-[(3S*-Hydroxypiperidin-4-(S*)yl)-sul- fanyl-acetyl]mutilin in the form of a hydrochloride (d-6-DMSO, 350K): 8.05(b, 3H, NH3 +), 4.25-4.1(m, 3H, CH2N, NHCHC═O), 3.75(m, 1H, CHO), 3.45-3.32(m, 3H, H11H22),
    #2.89(m, 1H, CHS), 1.18, 1.45(2xs, (CH3)15, (CH3)18), 0.9(m, 6H, CH(CH3)2), 0.75, 0.88(2xd, (CH3)16, (CH3)17, J=5.4Hz 0.98)
    Figure US20050215637A1-20050929-C00098
         		Example 43 14-O-[((Azepan-2-one-4-(R/S)-yl)-sulfanyl acetyl)]-mutilin (d6-DMSO): 6.15, 5.1(2xm, H19, H20, H21), 5.52(d, 1H, J=5, 2Hz, H14), 3.4(m, 1H, H11,), 3.3(m, 2H, H22), 3.1(m, 2H, SCH, CHN), 1.18, 1.45(2xs, (CH3)15, (CH3)18), 0.75, 0.88(2xd, (CH3)16, (CH3)17, J=5.4Hz)
    Figure US20050215637A1-20050929-C00099
         		Example 44 14-O-{[(3-Oxo-cyclopentan-(R/S)-yl)-sulfanyl]-acetyl}-mutilin (CDCl3): 6.45, 5.35, 5.2(3xm, H19, H20, H21), 5.8(d, 1H, H14, J=8.4Hz), 3.6(m, 1H, SCH), 3.35(m, 1H, 11-OH), AB-system(νA=3.25, νB=3.17, 2H, H22, J=14.8Hz), 2.65(m, ½H, H-2a′), 2.6(m, ½H, H-2a′), 1.45,
    #1.18(2xs, (CH3)15, (CH3)18), 0.9, 0.75(2xd, (CH3)16, (CH3)17, J=6.8Hz)
  • Novel compounds provided by the present invention, including compounds, the formulae of which are as set out in TABLE 1 to TABLE 5, and compounds of formulae IA, IB, IC, ID, IEX, I′EX and I″EX are herein designated as “novel compound(s) of (according to) the present invention”. “The residue of an amino acid”, whenever defined in any one of the novel compounds of the present invention includes that part of an amino acid, e.g. including natural and synthetic amino acids, e.g. valine and other amino acids as defined herein, most preferably valine; which remains if the hydroxy group from the carboxylic acid group is splitt off, e.g. In case of valine [HO—CO—CH(NH2)—CH(CH3)2] the residue —CO—CH(NH2)—CH(CH3)2.
  • In a novel compound of the present invention each single defined substitutent may be a preferred substituent, e.g. independently of each other substitutent defined.
  • A novel compound of the present invention includes a compound in any form, e.g. In free form, in the form of a salt, in the form of a solvate and in the form of a salt and a solvate.
  • In another aspect the present invention provides a novel compound of the present invention in the form of a salt.
  • A salt of a novel compound of the present invention includes a pharmaceutically acceptable salt, e.g. including a metal salt or an acid addition salt. Metal salts include for example alkali or earth alkali salts; acid addition salts include salts of a compound of formula I with an acid, e.g. hydrogen fumaric acid, fumaric acid, naphthalin-1,5-sulphonic acid, phosphoric acid, tartaric acid, citric acid, hydrochloric acid, deuterochloric acid; preferably hydrochloric acid. A novel compound of the present invention in free form may be converted into a corresponding compound in the form of a salt; and vice versa. A novel compound of the present invention in free form or in the form of a salt and in the form of a solvate may be converted into a corresponding compound in free form or in the form of a salt in unsolvated form; and vice versa.
  • A novel compound of the present invention may exist in the form of isomers and mixtures thereof; e.g. optical isomers, diastereoisomers, cis-, trans-conformers. A compound of the present invention may e.g. contain asymmetric carbon atoms and may thus exist in the form of diastereoisomeres and mixtures thereof, e.g. racemates. For example a novel compound of the present invention may comprise the residue of an amino acid. In such amino acid residue the carbon atom to which the amino group is attached may be an asymmetric carbon atom and the amino group attached may thus be in the R- or S-configuration. A novel compound of the present invention may comprise a cycloalkyl, e.g. attached to the sulfanyl group, which cycloalkyl may be further substituted, and said substitutents may exist in the cis or in the trans conformation. E.g., the carbon atom of a cycloalkyl group to which the sulfanylgroup is attached may be asymmetric, e.g. if said cycloalkyl Is further substituted, and substitutents attached to said cycloalkyl group may be in the R- or in the S-configuration. E.g., a novel compound of the present invention also may comprise an oxime group. The hydroxgroup attached to the imino group may be in syn- or in anti-configuration. Isomeric mixtures may be separated as appropriate, e.g. according, e.g. analogously, to a method as conventional, to obtain pure isomers. The present invention includes a novel compound of the present invention in any isomeric form and in any isomeric mixture. The present invention also includes tautomers of a novel compound of the present invention where such tautomers can exist.
  • Any compound described herein, e.g. a novel compound of the present invention, may be prepared as appropriate, e.g. according to a method as conventional, e.g. analogously, e.g. or as specified herein.
  • In another aspect the present invention provides a process for the production of a compound of formula
    Figure US20050215637A1-20050929-C00100
    wherein
    • R1P has the meaning of R1A as defined above,
    • R2P has the meaning of R2A as defined above,
    • R3P has the meaning of R6A as defined above,
    • the dotted line has the meaning as defined above, and
    • RP has the meaning as set out in any one of patent claims 6 to 11,
      comprising the steps
    • a. reacting a compound of formula
      Figure US20050215637A1-20050929-C00101
      wherein R1P and R2P are as defined above, and the doffed line has the meaning as defined above,
      with thiourea and subsequent reduction to obtain a compound of formula
      Figure US20050215637A1-20050929-C00102
      wherein R1P and R2P are as defined above,
    • b. reacting a compound of formula IIP obtained in step a, with a compound of formula
      RP—H
      • wherein RP is as defined above, in a reactive form, e.g. a mesylate or a tosylate, optionally in a protected form,
      • to obtain a compound of formula IP (i.e. novel compound of the present invention), or to obtain a pre-form of a compound of formula IP,
    • c. optionally further reacting a pre-form obtained in step b. to obtain a compound of formula IP, e.g. introducing deuterium to obtain a compound of formula IP wherein the subsitutents are as defined above, and
    • d. isolating a compound of formula IP obtained in step b. or in step c. from the reaction mixture.
    • RP is
      • substituted (C4-8)cycloalkyl,
      • substituted phenyl,
      • substituted aliphatic heterocyclyl, having 4 to 8 ring members and comprising as a heteroatom 1 or 2 nitrogen atoms,
      • alkyl, substituted by (substituted) amino,
      • alkyl substituted by heterocyclyl, or
      • substituted, bicyclic aliphatic heterocyclyl, comprising in each ring 5 ring members and one oxygen heteroatom,
        e.g. including the meanings of of RP in the novel pleuromutilins of the present invention, e.g. such as set out in patent claims 6 to 11.
  • A process provided by the present invention may e.g. be carried out analogously to processes as set out In any of the patent references cited herein, e.g. analogously to a process for the production of compounds as described in patent literature cited herein, such as in WO0109095, WO0204414 and WO0222580, or as described herein.
  • All patent references cited herein are introduced by reference, especially with respect to the claim scopes and meanings of the substituents, e.g. including the preferred meanings of the substitutents, and with respect to production processes.
  • A pleuromutilin of the present invention includes the novel compounds of the present invention of formula IP. A pleuromutilin of the present invention includes one or more, preferably one, pleuromutilins of the present invention, e.g. one pleuromutilin or a combination of different pleuromutlins of the present invention.
  • We have found that novel compounds of the present invention, including compounds of formula IP, exhibit pharmacological activity similar to pleuromutilins in similar indications as described in WO0109095, WO0204414 and WO0222580, e.g. in test systems similar as described in WO0109095, WO0204414 and WO0222580, and additionally in test systems described herein. Compounds of formula IP are therefore useful as pharmaceuticals.
  • In another aspect the present invention provides a compound of formula IP for use as a pharmaceutical, preferably as an antimicrobial, such as an antibiotic, e.g. and as an anti-anaerobic, including the use as a pharmaceutical in the treatment of diseases mediated by Mycobacterium, such as Mycobacterium tuberculosis.
  • In another aspect the present invention provides a compound of formula IP for use in the preparation of a medicament for the treatment of microbial diseases, for example of diseases mediated by bacteria, e.g. selected from Staphylococci, Streptococci, Enterococci, Mycobacterium, e.g. Mycobacterium tuberculosis; e.g. and of diseases mediated by Mycoplasms, Chlamydia and obligatory anaerobes.
  • In another aspect the present Invention provides a method of treatment of microbial diseases, for example of diseases mediated by bacteria, e.g. selected from Staphylococci, Streptococci, Enterococci, Mycobacterium, e.g. Mycobacterium tuberculosis; e.g. and of diseases mediated by Mycoplasms, Chlamydia and obligatory anaerobes, which method comprises administering to a subject in need of such treatment an effective amount of a noveel compound of the present invwention, e.g. including a compound of formula IP; e.g. in the form of a pharmaceutical composition.
  • Treatment includes treatment and prophylaxis.
  • For such treatment, the appropriate dosage will, of course, vary depending upon, for example, the chemical nature and the pharmakokinetic data of a compound of the present invention employed, the individual host, the mode of administration and the nature and severity of the conditions being treated. However, in general, for satisfactory results in larger mammals, for example humans, an indicated daily dosage is in the range from about 0.05 g to about 5.0 g, of a novel compound of of the present invention; conveniently administered, for example, in divided doses up to four times a day.
  • For treatment of diseases mediated by Mycobacterium with a pleuromutilin of the present invention similar considerations apply.
  • A novel compound of the present invention may be administered by any conventional route, for example enterally, e.g. including nasal, buccal, rectal, oral administration; parenterally, e.g. including intravenous, intramuscular, subcutanous administration; or topically; e.g. including epicutaneous, intranasal, intratracheal administration; e.g. in form of coated or uncoated tablets, capsules, injectable solutions or suspensions, e.g. in the form of ampoules, vials, in the form of creams, gels, pastes, inhaler powder, foams, tinctures, lip sticks, drops, sprays, or in the form of suppositories.
  • For administration of a pleuromutilin of the present invention in diseases mediated by Mycobacterium, similar considerations apply, with the exception of topical administration.
  • The novel compounds of the present invention may be administered in the form of a pharmaceutically acceptable salt, e.g. an acid addition salt or metal salt; or in free form; optionally in the form of a solvate. The novel compounds of the present invention in the form of a salt exhibit the same order of activity as the novel compounds of the present invention in free form; optionally in the form of a solvate.
  • For administration of a pleuromutilin of the present invention in diseases mediated by Mycobacterium similar considerations apply.
  • A novel compound of the present invention may be used for pharmaceutical treatment according to the present invention alone, or in combination with one or more other pharmaceutically active agents. Such other pharmaceutically active agents e.g. Include other antimicrobials, e.g. including antibiotics, e.g. cephalosporins, penicillins, erythromycins, tetracyclines.
  • Similar considerations apply for a pleuromutilin of to the present invention in diseases mediated by Mycobacterium, but appropriate other pharmaceutically active agents includes agents known to be active in the treatment of diseases mediated by Mycobacterium, such as Rifampicin (Rifampicin®), Streptomycin (Streptomycin®), Ethambutol (Ethambutol®), Pyrizinamid (Pyrizinamid®).
  • Combinations include fixed combinations, in which two or more pharmaceutically active agents are in the same formulation; kits, in which two or more pharmaceutically active agents in separate formulations are sold in the same package, e.g. with instruction for co-administration; and free combinations in which the pharmaceutically active agents are packaged separately, but instruction for simultaneous or sequential administration are given.
  • In another aspect the present invention provides a pharmaceutical composition comprising a novel compound of the present invention, e.g. a compound of formula IP, in association with at least one pharmaceutical excipient, e.g. appropriate carrier and/or diluent, e.g. including fillers, binders, disintegrators, flow conditioners, lubricants, sugars and sweeteners, fragrances, preservatives, stabilizers, wetting agents and/or emulsifiers, solubilizers, salts for regulating osmotic pressure and/or buffers; e.g. further comprising another pharmaceutically active agent.
  • Pharmaceutical composition comprising pleuromutilins of the present invention for administration in diseases mediated by Mycobtarium may comprise similar excipient as described above.
  • In another aspect the present invention provides a pharmaceutical composition comprising a pleuromutilin of the present invention in association with at least one pharmaceutical excipient, and further comprising another pharmaceutically active agent useful in the treatment of Mycobacterium, e.g. M. tuberculosis, infections, such as Rifampicin (Rifampicin®), Streptomycin (Streptomycin®), Ethambutol (Ethambutol®), Pyrizinamid (Pyrizinamid®).
  • Such compositions may be manufactured according, e.g. analogously to a method as conventional, e.g. by mixing, granulating, coating, dissolving or lyophilzing processes. Unit dosage forms may contain, for example, from about 0.5 mg to about 1500 mg, such as 1 mg to about 500 mg.
  • Pleuromutilins of the present invention for administration in diseases mediated by Mycobtarium tuberculosis may be administered in a similar mode and in similar dosages as Rifampicin or Streptomycin.
  • A pleuromutilin of the present invention is preferably selected from the group consisting of a compound of formula I—U.S. Pat. No. 4,278,674, a compound of formula I—EP0153277, a compound of formula I—WO0109095, a compound of formula I—WO0204414, a compound of formula I—WO0222580, a compound of TABLE 1, a compound of formula IB, or a of formula ID; e.g. including
      • a compound of formula I-Tiamulin,
      • a compound of formula I-Valnemulin, e.g. in the form of a hydrochloride;
      • a compound of formula
        Figure US20050215637A1-20050929-C00103
        e.g. in the form of a hydrochloride;
      • a compound of formula
        Figure US20050215637A1-20050929-C00104
        e.g. in the form of a hydrochloride,
      • a compound of formula
        Figure US20050215637A1-20050929-C00105
        e.g. in the form of a hydrochloride,
      • a compound of formula
        Figure US20050215637A1-20050929-C00106
        e.g. in the form of a hydrochloride,
      • a compound of formula
        Figure US20050215637A1-20050929-C00107
        e.g. in the form of a hydrochloride,
      • a compound of formula
        Figure US20050215637A1-20050929-C00108
        e.g. in the form of a hydrochloride,
      • a compound of formula
        Figure US20050215637A1-20050929-C00109
        and
      • a compound of
        Figure US20050215637A1-20050929-C00110
        e.g. in the form of a hydrochloride.
  • Activity against strains of Mycobacterium, e.g. M. tuberculosis may be determined according to the following General Test Procedure:
  • General Test Procedure
  • Is carried out according to the known and appropriate Agar Dilution Test.
  • Agar is used as a substrate. Shortly before solidifaction of the Agar TEST COMPOUNDS In different concentrations are added and mixed into the still liquid agar mass (according to the Agar dilution test). Controls without TEST COMPOUNDS are also prepared for determination of strain growth ability. The thus prepared agars are inoculated after solidification with Mycobacterium tuberculosis strains. Incubation is carried out in normal incubators at 37° C. As a nutrition medium Middlebrook 7H10+OADC (Oleic, Albumin, Dextrose, Catalase) Enrichment (pH 6.71-6.73) is used.
  • The minimum inhibition concentration (MIC) which is the compound concentration in the agar which inhibits 99% of strain growth, is determined after 3 weeks, 4 weeks and 5 weeks after inoculation.
  • Pleuromutilins of the present invention show activity against strains of Mycobacterium, e.g. M. tuberculosis and are thus useful in the treatment of infectios caused by Mycobacterium. Pleuromutilins of the present invention surprisingly are even active against resistant and multiresistant M. tuberculosis strains, e.g. strains which are resistant against treatment with known pharmaceuticals useful in the treatment of tuberculosis, e.g. Isoniacid, Rifampicin, Streptomycin.
  • In the following Examples all temperatures are in degree Centigrade and are uncorrected. The following abbreviations are used:
    • BOC: tert.butoxyxcarbonyl DCC: dicyclohexylcarbodiimide
    • DMF: N,N-dimethylformamide DMSO: dimethylsulfoxide
    • EDC: N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride
    • EtAc: ethyl acetate EtOH: ethanol
    • HOBT: 1-hydroxybenzotriazole MeOH: methanol
    • MS: mass spectroscopy RT: room temperature
    • TBAF: tetra-n-butylammonium fluoride TFA: trifluoroacetic acid
    • THF: tetrahydrofurane
    • Chromatography is carried out on silica gel.
    PREPARATION EXAMPLES I. Preparation of (Novel) Pleuromutilins of the Present Invention Example I-A 14-O-[(N-(3-Methyl-2(R)-amino-butyryl)-piperidine-3-yl)-sulfanylacetyl]-mutilin in the form of a hydrochloride I-AA) 14-O-[(N-BOC-Piperidin-3(S)-yl)-sulfanylacetyl]-mutilin
  • Method 1:
  • 532 mg of 22-O-tosyl-pleuromutilin are added to a solution of 217 mg of N-BOC-piperidine-3(S)-thiol and 112 mg potassium tert.butylate in 10 ml of THF, the mixture obtained is stirred forer 3 hours, the mixture obtained is distributed between EtAc and brine, the organic phase obtained is dried, solvent is evaporated and the evaporation residue obtained is subjected to chromatography. 14-O-[(N-BOC-Piperidin-3(S)-yl)-sulfanylacetyl]-mutilin is obtained.
  • Method 2:
  • A solution of 1.97 g of 22-mercapto-pleuromutilin, 1.39 g of N-BOC-3(R)-methylsulfonyloxy-piperidine and 0.12 g of sodium in 50 ml of EtOH is heated to 90° for 12 hours, from the mixture obtained solvent is evaporated and the evaporation residue is subjected to chromatography. 14-O-C-[(N-BOC-piperidin-3-yl)-sulfanylacetyl]-mutilin is obtained. 1H-MR(CDCl3): 6.45, 5.35, 5.2(3xm,H19,H20,H21), 5.74(d,1H,5.2 Hz,H14), 3.35(d, 1H,H11,J=5.2 Hz), AB-system: 3.12,3.18, J=14.7 Hz,H22), 3.2, 2.95, 2.65, 2.6(4xm,CH2NCH2), 2.85 (m,1H, SCH), 1.18,1.45 (2×s,(CH3)15,(CH3)18), 0.75,0.88(2xd,(CH3)16, (CH3)17,J=5.4 Hz)
    • I-AB) 14-O-[(N-(3-Methyl-2(R)-amino-butyryl)-piperidine-3-yl)-sulfanylacetyl]-mutilin in the form of a hydrochloride
  • A solution of 280 mg of 14-O-[(N-BOC-piperidin-3-yl)-sulfanylacetyl]-mutilin in 20 ml of CH2Cl2 and 1 ml of TFA is stirred at RT for 30 minutes and from the mixture obtained solvent is evaporated. The evaporation residue obtained is treated with 40 ml of CH2Cl2, 55 mg of N-methyl-morpholine, 110 mg of N-BOC-(R)-valine and 105 mg of DCC are added and the mixture obtained is stirred for 3 hours. From the mixture obtained precipitated dicyclohexylurea is filtered off and the filtrate obtained is subjected to chromatography. Purified 14-O-[(N-(3-Methyl-2(R)-amino-butyryl)-piperidine-3-yl)-sulfanylacetyl]-mutilin obtained is treated with TFA in CH2Cl2, solvent is evaporated and the evaporation residue obtained is treated with etheric HCl. 14-O-[(N-(3-Methyl-2(R)-amino-butyryl)-piperidine-3-yl)-sulfanylacetyl]-mutilin in the form of a hydrochloride is obtained.
  • 1H-NMR (d-6-DMSO,330 K): 6.45, 5.35, 5.2(3xm,H19,H20,H21), 5.74 (d,1H,5.2 Hz,H14), 5.45 (d,1H,NH,J=7.8 Hz), 4.1 (m,1H,NHCHCO), 3.35 (d,1H,H11,J=5.2 Hz), AB-system: 3.12,3.18, J=14.7 Hz,H22), 3.2, 2.95, 2.65, 2.6 (4xm,CH2NCH2), 2.8 (m,1H,SCH), 1.18,1.45 (2xs,(CH3)15,(CH3)18), 0.75, 0.88 (2xd,(CH3)16,(CH3)17,J=5.4 Hz), 0.78,0.84 (2xd, (CH3)2CHJ=6.8 Hz)
    • Example I-B 14-O-[(N-(3-Methyl-2(R)-amino-butyryl)-piperidine-3(S)-yl)-sulfanylacetyl]-2(S)-fluoro-mutilin in the form of a hydrochloride IBA) 14-O-(Tosyloxyacetyl)-2(S)-fluoro-mutilin
  • To a solution of 500 mg of 14-O-(hydroxyacetyl)-2(S)-fluoro-mutilin (see e.g. Vyplel H., et al J Fluorine Chem; 23, 482 (1983)), in 5 ml of CH2Cl2 450 mg of toluene sulfonic acid anhydride and 0.21 ml of pyridine are added and the mixture obtained is stirred for 4 hours at RT. The mixture obtianed is diluted with CH2Cl2 and extracted with 1N HCl, aqueous NaHCO3 and H2O. The organic phase obtained is dried, solvvent is evaporated and the evaporation residue is subjected to chromatography. 14-O-[tosyloxyacetyl]-2(S)-fluoro-mutilin is obtained.
    • IBB) 14-O-[(N-(3-Methyl-2(R)-amino-butyryl)-piperidine-3(S)-yl)-sulfanylacetyl]-2(S)-fluoro-mutilin in the form of a hydrochloride
  • is obtained starting from 14-O-[tosyloxyacetyl]-2(S)-fluoro-mutilin analogously to the method of Example IAB). Characterisation data see TABLE 1, Example 12.
    • Example I-C 14-O-[(3-Guanidino-phenylsulfanyl)-acetyl]mutilin in the form of a hydrochloride ICA) 14-O-[(3-Amino-phenylsulfanyl)-acetyl]-mutilin
  • A solution of 0.92 g of sodium and 5 g of 3-amino-thiophenol in 100 ml of dry EtOH is added to a solution of 21.3 g of 22-O-tosyl-pleuromutilin (see e.g. H. Egger et al., J. Antibiotics 29, 923 (1976)) in 250 ml of ethylmethylketone at 25° under careful temperature control. The mixture obtained is kept for 15 hours at 25°, filtered and the filtrate obtained is concentrated under reduced pressure and subjected to chromatography. 14-O-[(3-Amino-phenylsulfanyl)-acetyl]-mutilin is obtained. 1H-NMR(CDCl3): 0.58(d, 3H, H16, J=7.2 Hz), 0.81 (d, 3H, H17, J=7.3 Hz), 1.02 (s, 3H, H18), 1.32 (s, 3H, H15), ABX-system(νA=1.2, νB=1.88, H13a,H13b, J=16.1 Hz, J=9.1 Hz), 2.08(d,1H, H4, J=2.1 Hz), ABXY-system(νA=2.23, νB=2.19, H2a,H2b, J=16.2 Hz, J=9.1 Hz, J=1.8 Hz), 2.3(m,1H,H10), 3.4 (d,1H,H11, J=5.98 Hz), AB-system (νA=3.81, νB=3.89, 2H, H22, J=14.1 Hz), 5.18(dd,1H, H20a, J=17.5 Hz, J=1.6 Hz), 5.29(dd,1H, H20b, J=11 Hz, J=1.6 Hz), 5.51 (d,1H, H14, J=8.3 Hz), 6.05 (dd,1H,H19, J=11 Hz, J=17.5 Hz), 7.0 (m,1H, arom.H), 7.18 (m2H, arom.H), 7.3t,1H, arom.H5, J=8 Hz).
    • ICB) 14-O-[(3-Guanidino-phenylsulfanyl)-acetyl]mutilin in the form of a hydrochloride
  • A solution of 2.4 g of 14-O-[(3-amino-phenylsulfanyl)-acetyl]mutilin, 1.5 g of cyanamide and 0.44 ml of HCl conc. in 20 ml of dioxane is stirred at room temperature for 28 hours. 14-O-[(3-Guanidino-phenylsulfanyl)-acetyl]mutilin in the form of a hydrochloride in crystalline form is obtained. Characterisation data see in TABLES above.
    • Example I-D 14-O-[(3R*-Hydroxypiperidin-4-(R*)yl)-sulfanyl-acetyl]mutilin and 14-O-[(3S*-Hydroxypiperidin-4-(S*)yl)-sulfanyl-acetyl]mutilin in the form of a hydrochloride (diastereoisomeric mixture)
  • 1.06 g of pleuromutilin-22-O-tosylate dissolved in 1 ml of 2-butanone are slowly added to a solution of 466 mg of N-BOC-3-hydroxy-piperidin-4-thiol and 224 mg of potassium-tert.butylate in 20 ml of THF, the mixture obtained is stirred for 2 hours, the mixture obtained is distributed between brine and EtAc, extracted with 0.1N HCl, and the phases obtained are spearated. The organic phase obtained is dried, and the evaporation residue obtained is subjected to chromatography. A mixture of 14-O-[(3R*-Hydroxypiperidin-4-(R*)yl)-sulfanyl-acetyl]mutilin and 14-O-[(3S*-Hydroxypiperidin-4-(S*)yl)-sulfanyl-acetyl]mutilin is obtained which is treated with etheric HCl to obtain the corresponding hydrochloride. Characterisation data see in TABLE 5, Example 42.
    • Example I-E 2,2,4-Trideutero-14-O-[((3-(S*)-hydroxy-piperidine-4-(S*)-yl)sulfanyl)-acetyl]mutilin in the form of a deuterochloride
  • A solution of 300 mg of the compound obtained in Example ID in 30 ml dioxane with 5 ml of DCI (20% in D2O) is kept for 6 days at 25°. From the mixture obtained solvent is evaporated and the concentration resiue is subjected to lyophilization. 2,2,4-Trideutero-14-O-[((3-(S*)-hydroxy-piperidine-4-(S*)-yl)sulfanyl)-acetyl]mutilin in the form of a deuterochloride is obtained. Characterisation data see TABLE 5, Example 41.
    • Example I-F 14-O-[3-(R*)-((N-BOC-(R) Valyl-amino-cyclohexan-1-(R*)-yl)sulfanyl)-acetyl]mutilin (a) 14-O-[3-(S*)-((N-BOC-(R)-Valyl-amino-cyclohexan-1-(S*)-yl)sulfanyl)-acetyl]mutilin (b) 14-O-[3-(S*)-((N-BOC-(R)-Valyl-amino-cyclohexan-1-(R*)-yl)sulfanyl)-acetyl]mutilin (c) 14-O-[3-(R*)-((N-BOC-(R)-Valyl-amino-cyclohexan-1-(S*)-yl)sulfanyl)-acetyl]mutilin (d)
  • 2.66 g of pleuromutilin-22-O-tosylate dissolved in 10 ml THF are slowly added to a solution of 1.65 g of 3-(N-BOC-(R)-valyl-amino)-cyclohexane-(R/S)-thiol and 560 mg of potassium-tert.butylate in 25 ml of THF, the mixture obtained is stirred for 2 hours and distributed between brine and EtAc. The mixture obtained is extracted with 0.1N HCl, the organic phase obtained is dried, solvent is evaporated and the evaporation residue obtained is subjected to chromatography. Pure
    • (a) 14-O-[3-(R*)-((N-BOC-(R)-Valyl-amino-cyclohexan-1-(R*)-yl)sulfanyl)-acetyl]mutilin
    • (b) 14-O-[3-(S*)-((N-BOC-(R)-Valyl-amino-cyclohexan-1-(S*)-yl)sulfanyl)-acetyl]mutilin
    • (c) 14-O-[3-(S*)-((N-BOC-(R)-Valyl-amino-cyclohexan-1-(R*)-yl)sulfanyl)-acetyl]mutilin, and
    • (d) 14-O-[3-(R*)-((N-BOC-(R)-Valyl-amino-cyclohexan-1-(S*)-yl)sulfanyl)-acetyl]mutilin is obtained. 1H-NMR(d6-DMSO):
    • (a): 6.5(d,1H,NH,J=8.1 Hz), 6.15, 5.1 (2xm,H19,H20,H21), 5.52(d,1H,J=5,2 Hz,H14), 3.4(m,1H, H11), 3.55 (m,1H,CHN), 3.7 (m,α-valyl), 3.2 (m,2H,H22), 2.7 (m,1H,SCH), 1.4 (s,9H, tert.butyl), 1.18, 1.45(2xs,(CH3)15(CH3)18), 0.75,0.88(2xd,(CH3)16,(CH3)17,J=5.4 Hz).
    • (b): 6.15, 5.1 (2xm,H19,H20,H21), 5.52(d,1H,J=5,2 Hz,H14), 3.4(m,1H,H11), 3.55 (m,1H,CHN), 3.70 (m,α-valyl), 3.2 (m,2H,H22), 2.7 (m,1H,SCH), 1.4 (s,9H,tert.butyl), 1.18, 1.45(2xs, (CH3)15,(CH3)18), 0.75, 0.88 (2xd,(CH3)16,(CH3)17,J=5.4 Hz).
    • (c): 6.15, 5.1 (2xm,H19,H20,H21), 5.52(d,1H,J=5,2 Hz,H14), 3.4(m,1H,H11), 3.9 (m,1H,CHN), 3.75 (m,α-valyl), 3.2 (m,2H,H22), 3.15 (m,1H,SCH), 1.4 (s,9H,tert.butyl), 1.18, 1.45(2xs, (CH3)15,(CH3)18), 0.75, 0.88 (2xd,(CH3)16,(CH3)17,J=5.4 Hz).
    • (d): 6.15, 5.1 (2xm,H19,H20,H21), 5.52(d,1H,J=5,2 Hz,H14), 3.4(m,1H,H11), 3.9 (m,1H,CHN), 3.70 (m,α-valyl), 3.2 (m,2H,H22), 3.15 (m,1H,SCH), 1.4 (s,9H,tert.butyl), 1.18, 1.45(2xs, (CH3)15,(CH3)18), 0.75, 0.88 (2xd,(CH3)16,(CH3)17,J=5.4 Hz).
    Example I-G 14-O-[3-(R*)-((R)-Valyl-amino-cyclohexan-1-(R*)-yl)-sulfanyl)-acetyl]-mutilin and 14-O-[3-(S*)-((R)-Valyl-amino-cyclohexan-1-(S*)-yl)-sulfanyl)-acetyl]-mutilin in the form of a hydrochloride (mixture of trans-diastereoisomers)
  • 620 mg of a 1:1 mixture of 14-O-[3-(R*)-((N-BOC-(R)-valyl-amino-cyclohexan-1-(R*)-yl)sulfanyl)-acetyl]mutilin and 14-O[3-(S*)-((N-BOC-(R)-valyl-amino-cyclohexan-1-(S*)-ylsulfanyl)-acetyl]mutilin are dissolved in a mixture of 10 ml of dry etheric HCl and 10 ml of CH2Cl2. The mixture is stirred for 5 hours and a mixture of trans-diastereoisomers of 14-O-[3-(R*)-((R)-valyl-amino-cyclohexan-1-(R*)-yl)-sulfanyl)-acetyl]-mutilin of 14-O-[3-(S*)-((R)-valyl-amino-cyclohexan-1-(S*)-yl)sulfanyl)-acetyl]-mutilin in the form of a hydrochloride is obtained and isolated. 1H-NMR(d6-DMSO): Rotamer. 8.4 (m,1H,C═ONH), 8.15 (b,3H,NH3 +), 6.15, 5.1 (2xm,H19,H20,H21), 5.52(d,1H,J=5.2 Hz,H14), 3.95 (m, 1H, CHNH3 +, 3.4(m, 1H,H11), 3.55 (m,α-valyl), 3.2-3.3 (m,2H,H22), 3.18 (m,1H,SCH), 1.18, 1.45(2xs,(CH3)15,(CH3)18), 0.9 (m,6H,CH(CH3)2), 0.75, 0.88 (2xd,(CH3)16,(CH3)17,J=5.4 Hz)
    • Example I-H 14-O-[3-(R*)-((R)-Valyl-amino-cyclohexan-1-(S*)-yl)sulfanyl)-acetyl]-mutilin and 14-O-[3-(S*)-((R)-Valyl-amino-cyclohexan-1-(R*)-yl)-sulfanyl)-acetyl]-mutilin in the form of a hydrochloride (mixture of cis-diastereoisomers)
  • is obtained analagosouly to the method of Example I-G, but using a 1:1 mixture of 14-O-[3-(R*)-((N-BOC-(R)-valyl-amino-cyclohexan-1-(S*)-yl)sulfanyl)-acetyl]mutilin and 14-O-[3-(S*)-((N-BOC-(R)-valyl-amino-cyclohexan-1-(R*)-yl sulfanyl)-acetyl]mutilin as a starting material. 1H-NMR(d6-DMSO): Rotamers. 8.52 (m,1H,C═ONH), 8.2 (b,3H,NH3 +), 6.15, 5.1 (2xm,H19, H20,H21), 5.52(d,1H,J=5.2Hz,H14), 3.58 (m1H, CHNH3 +, 3.4(m,1H,H11), 3.48 (m,α-valyl), 3.2-3.3 (m,2H,H22), 2.75 (m,1H,SCH), 1.18, 1.45(2xs,(CH3)15,(CH3)18), 0.9 (m,6H,CH(CH3)2), 0.75, 0.88 (2xd,(CH3)16,(CH3)17,J=5.4 Hz)
    • Example I-I 14-O-[((N-(R)-Valyl-azepan-4-(R/S)-yl)-sulfanyl acetyl)]-mutilin in the form of a hydrochloride I-IA) 4-(R/S)-(2,4,6-Trimethyl-benzyl-sulfanyl)-azepan-2-one
  • A solution of 828 mg of 3-(R/S)-(2,4,6-trimethyl-benzyl-sulfanyl)cyclohexanone-oxime and 570 mg of toluenesulfonylchloride in 5 ml of pyridine is stirred at RT for 4 hours and additional 2 hours at 60°. The mixture obtained is distributed between diluted sulfuric acid (2 ml H2SO4 conc. in 15 ml H2O) and CH2Cl2, the organic phase obtained is dried, solvent is evaporated and the evaporation residue is subjected to chromatography. 4-(R/S)-(2,4,6-Trimethyl-benzyl-sulfanyl)-azepan-2-one is obtained. 1H-NMR(d-6-DMSO): 7.5 (m, 1H,NHCO), 6.8 (s,2H,arom.H), 3.75 (s,2H,C6H5CH2S—),3.2 (m,1H,CHN), 3.1 (m,3H, CH2NH,CHS) AB-system: vA=2.72, vB=2.65(2H,CH2C═O, J=13.4 Hz, J=4.5 Hz) 2.13 2.15, 2.3(9H, 3xCH3)
    • I-IB) 4-(R/S)-(2,4,6-Trimethyl-benzyl-sulfanyl)-azepane
  • 3.3 g of 4-(R/S)-(2,4,6-trimethyl-benzyl-sulfanyl)-azepan-2-one are added to a mixture of 15 ml of a 1M-solution of LiAlH3 and 50 ml THF are added. The mixture obtained is heated for 1 hour at 80°, poured into 200 ml of a 20% aqueous NH4Cl-solution and the mixture obtained is extracted with EtAc. The organic phase obtained is dried and solvent is evaporated. 4-(R/S)-(2,4,6-Trimethyl-benzyl-sulfanyl)-azepane is obtained.
    • I-IC) N-BOC-(4-(R/S)-(2,4,6-Trimethyl-benzyl-sulfanyl))-azepane
  • A solution of 2.63 g of 4-(R/S)-(2,4,6-trimethyl-benzyl-sulfanyl)-azepane, 2.18 g of BOC-anhydride and 1 g of triethylamine in 100 ml of THF is stirred at 25° for 12 hours and from the mixture obtained solvent is evaporated. The evaporation residue obtained is distributed between CH2Cl2 and 1M HCl. Solvent from the organic phase obtained is evaporated and the evaporation residue is subjected to chromatography. N-BOC-(4-(R/S)-(2,4,6-trimethyl-benzyl-sulfanyl))-azepane is obtained. 1H-NMR(d6-DMSO): 6.8 (s,2H,arom.H), 3.75 (s,2H,C6H5CH2S—), 3.2-3.5(m,4H,CH2NHCH2), 2.9(m,1H,CHS), 2.13 2.15, 2.3(9H, 3xCH3)
    • I-IC) N-BOC(R/S)-Azepan-thiol
  • is obtained analogously to the method of Example II-D, but using appropriate starting materials.
    • I-ID) 14-O-[((N-BOC-Azepan-4-(R/S)-yl)-sulfanyl acetyl)]-mutilin
  • A solution of 1.06 g of pleuromutilin-22-O-tosylate dissolved in 10 ml THF is slowly added to a solution of 420 mg of N-BOC-(4-(R/S)-azepane-thiol and 220 mg of potassium-tert.butylate in 25 ml of THF and the mixture obtained is stirred for 2 hours. The mixture obtained is distributed between brine and EtAc, the mixture obtained is extracted with 0.1N HCl, the organic phase obtained is dried, solvent is evaporated and the evaporation residue obtained is subjected to chromatography. 14-O-[((N-BOC-azepan-4-(R/S)-yl)-sulfanyl acetyl)-mutilin is obtained. 1H-NMR(d6-DMSO): 6.15, 5.1 (2xm,H19,H20,H21), 5.52(d,1H,J=5.2 Hz,H14), 4.52 (d,1H,OH,J=6.2 Hz) 3.4(t,1H,H11,J=6.2 Hz), 3.1-3.4 (m,6H,H22,CH2NCH2), 2.9 (m,1H,SCH), 1.4 (s,9H,tert.butyl),1.18, 1.45(2xs,(CH3)15,(CH3)18), 0.75, 0.88 (2xd,(CH3)16,(CH3)17,J=5.4 Hz)
    • I-IE) 14-O-[((Azepan-4-(R/S)-yl)-sulfanyl acetyl)]-mutilin in the form of a hydrochloride
  • 400 mg of 14-O-[((N-BOC-azepan-4-(R/S)-yl)-sulfanyl-acetyl)-mutilin are dissolved in a mixture of 10 ml of dry etheric HCl and 10 ml of CH2Cl2. The mixture obtained is stirred for 5 hours and 14-O-[((azepan-4-(R/S)-yl)-sulfanyl acetyl)]-mutilin in the form of a hydrochloride is isolated. Characterisation data see TABLE 1, Example 14.
    • I-IF) 14-O-[((N-(R)-Valyl-azepan-4-(R/S)-yl)-sulfanyl acetyl)]-mutilin in the form of a hydrochloride
  • A mixture of 245 mg 14-O-[((azepan-4-(R/S)-yl)-sulfanyl acetyl)]-mutilin in the form of a hydrochloride, 110 mg of BOC-R-valin, 95 mg of EDC and 100 mg of triethylamine in 10 ml THF is stirred at RT for 2 hours. The mixture obtained is distributed between brine and EtAc, the organic phase obtained is dried, solvent is evaporated and the evaporation residue obtained is subjected to chromatography.
  • 14-O-[((N-BOC-(R)-Valyl-azepan-4-(R/S)-yl)-sulfanyl acetyl)]-mutilin-hydrochloride is obtained. The BOG-protecting group is cleaved by treatment with 5 ml of etheric HCl and 14-O-[((N-(R)-Valyl-azepan-4-(R/S)-yl)sulfanyl acetyl)]-mutilin in the form of a hydrochloride is obtained. Characterisation data see TABLE 1, Example 15.
    • Example I-J 14-O-[((Azepan-2-one-4-(R/S)-yl)-sulfanyl acetyl)]-mutilin
  • is obtained analagously to the method of Example IAB), starting from 4-(R/S)-mercapto-azepan-2-one. Characterisation data see TABLE 5, Example 43.
    • Example I-K 14-O-{[(3-Oxo-cyclopentan-(R/S)-yl)-sulfanyl]-acetyl}-mutilin
  • A solution of 3.95 g of 14-mercapto-acetyl-mutilin in 5 ml of pyridine is treated with 0.81 g of cyclopent-2-enone and a catalytical amount of triethylamine. The mixture obtained is stirred for 3 hours at room temperature, diluted with EtAc and extracted with 1N HCl and H2O. The organic phase obtained is dried, solvent is evaporated and the evaporation residue obtained is subjected to chromatography. 14-O-{[(3-oxo-cyclopentan-(R/S)-yl)-sulfanyl]-acetyl}-mutilin is obtained. Characterisation data see in TABLE 5, Example 44.
    • Example I-L 14-O-{[(3-Hydroxyimino-cyclopentan-(R/S)-yl)-sulfanyl]-acetyl}-mutilin (syn and anti forms)
  • 3.88 g of 14-O-{[(3-oxo-cyclopentan-(R/S)-yl)-sulfanyl]-acetyl}-mutilin are stirred overnight with 566 mg of hydroxylamine hydrochloride and 1.13 ml of triethylamine in 40 ml of DMF. From the mixture obtained solvent is distilled off, the distillation residue obtained is taken up in EtAc and the mixture obtained is extracted with 0.1N HCl and brine. The organic phase obtained is dried and solvent is evaporated. A mixture of 14-O-{[(3-hydroxyimino-cyclo-pentan-(R/S)-yl)-sulfanyl]-acetyl}-mutilin in the syn*- and in the anti*-form is obtained which mixture is either separated by chromatography to obtain the pure syn- and the pure anti-forms, or is used in the form of the mixture obtained in further reaction steps. Characterisation data see TABLE 3, Example 24.
    • Example I-M 14-O-{[(3-(2-Diethylamino-ethoxyimino)-cyclopentan-(R/S)-yl)sulfanyl]-acetyl}-mutilin in the form of a hydrochloride
  • 200 mg of 14-O-{[(3-hydroxyimino-cyclopentan-(R/S)-yl)-sulfanyl]-acetyl}-mutilin and 70 mg of diethylaminoethylchloride hydrochloride are stirred in 5 ml of CH2Cl2, 90 mg of potassium tert butoxide are added and stirring is continued for 2 days at RT. From the mixture obtained solvent is evaporated, the evaporation residue is subjected to chromatography, the relevant chromatographic fractions obtained are distributed between Et2O and 0.1N HCl and the aqueous layer is lyophilized. 14-O-{[(3-(2-diethylamino-ethoxyimino)-cyclopent-(R/S)-yl)-sulfanyl]-acetyl-}mutilin hydrochloride (syn/anti mixture) is obtained. Characterisation data see TABLE 3, Example 26.
    • Example I-N 14-O-[(2-(R*)-((R)-Valyl)-amino-cyclohexan-1-(R*)-yl)-sulfanyl acetyl)]-mutilin-hydrochloride I-NA) 14-O-[((2-(R*)-Aminocyclohexan-1-(R*)-yl)sulfanyl acetyl)]-mutilin
  • 1.06 g of pleuromutilin-22-O-tosylate dissolved in 5 ml of THF are slowly added to a solution of 334 mg of 2-(R*)-aminocyclohexan-(R*)-thiol in the form of a hydrochloride (see e.g. G. Kavadias and R. Droghini, Can. J. Chem. 1978, 56, 2743) and 92 mg sodium in 50 ml of EtOH, the mixture obtained is stirred for 2 hours, distributed between brine and EtAc, extracted with 0.1N HCl and dried. From the mixture obtained solvent is evaporated and the evaporation residue is subjected to chromatography. 14-O-[((2-(R*)-aminocyclohexan-1-(R*)-yl)-sulfanyl acetyl)]-mutilin is obtained. 1H-NMR(d6-DMSO): 6.15, 5.1 (2xm,H19,H20,H21), 5.52(d,1H,J=5.2 Hz,H14), 2.45(m,1H,CHNH), 3.21 (s,2H,H22), 3.4(d,1H, H11,J=5 Hz), 2.55(m,1H,CHS), 1.18, 1.45 (2xs,(CH3)15,(CH3)18), 0.9 (m,6H,CH(CH3)2), 0.75, 0.88 (2xd,(CH3)16,(CH3)17,J=5.4 Hz).
    • I-NB) 14-O-[(2-(R*)-((R)-Valyl)-amino-cyclohexan-1-(R*)-yl)-sulfanyl acetyl)]-mutilin in the form of a hydrochloride
  • A mixture of 245 mg of 14-O-[((2-(R*)-aminocyclohexan-1-(R*)-yl)-sulfanyl acetyl)]-mutilin, 110 mg of BOC-(R)-valin, 95 mg of EDC and 68 mg of HOBT in 10 ml of THF is stirred at RT for 2 hours. The mixture obtained is distributed between brine and EtAc, the organic phase obtained is dried, solvent is evaporated and the evaporation resiude is subjected to chromatography. 14-O-[((2-(R*)-(N-BOC-(R)-Valyl)-amino-cyclohexan-1-(R*)-yl)-sulfanyl acetyl)]-mutilin is obtained. The BOC-protecting group is cleaved by treatment with 5 ml of etheric HCl and 14-O-[(2-(R*)-((R)-valyl)-amino-cyclohexan-1-(R*)-yl)-sulfanyl acetyl)]-mutilin in the form of a hydrochloride is obtained. 1H-NMR(d6-DMSO): Diastereoisomers): 8.45(m, 1H, NHC=O), 8.1(b,3H,NH3 +), 6.15, 5.1 (2xm,H19,H20,H21), 5.52(d,1H,J=5.2 Hz,H14), 3.55 (m,1H, a-H-valyl), 3.60(m,1H,CHNH), 3.26-3.35 (m,2H,H22), 3.4(m,1H, H11), 4.5 (d,1H,OH, J=6.2 Hz), 2.6, 2.75(2xm,1H,CHS), 1.25 (b,3H,CH3CS), 1.18, 1.45(2xs,(CH3)15,(CH3)18), 0.9 (m,6H,CH(CH3)2), 0.75, 0.88 (2xd,(CH3)16,(CH3)17,J=5.4 Hz)
    • Example I-O 14-O-[[(3S,3aS,6S,6aR)-6-Amino-hexahydro-furo[3,2-b]furan-3-ylsulfanyl]-acetyl]-mutilin hydrochloride I-OA) Toluene-4-sulfonic acid (3R, 3aS, 6R, 6aR)-6-hydroxy-hexahydro-furo[3,2-blfuran-3-yl ester
  • A solution of 5 g of (3R, 3aS, 6R, 6aR)-hexahydro-furo[3,2-b]furan-3,6-diol in 50 ml of pyridine is stirred for 16h with 7.8 g of toluenesulfonylchloride. From the mixture obtained solvent is distilled off and the distillation residue obtained is dissolved in EtAc and extracted with 1N HCl, saturated aqueous NaHCO3-solution and H2O. The organic layer obtained is dried, solvent is evaporated and the evaporation residue is subjected to chromatography. Toluene-4-sulfonic acid (3R, 3aS, 6R, 6aR)-6-hydroxy-hexahydro-furo[3,2-b]furan-3-yl ester is obtained. 1HNMR(DMSO-d6): 7.8 (d,2H,Ar—H,J=8.6 Hz), 7.5 (d,2H,Ar—H,J=8.6 Hz), 4.8-4.9 (m,2H,H-3, 6-OH), 4.4 (dd,1H,H-3a,J=4.7 and 5.0 Hz), 4.2 (dd,1H,H-6a,J=4.7 and 4.8 Hz), 3.9-4.0 (m,1H,H-6), 3.7-3.8 (m,2H,H-2 and H-5), 3.6 (d,1H,H-2,J=9.3 and 7.1 Hz), 3.2-3.4(m,1H,H-5), 2.4 (s, 3H, Ar—CH3).
    • I-OB) (3R, 3aR, 6S, 6aR)-6-Azido-hexahydro-furo[3,2-b]furan-3-ol
  • A solution of 2.5 g of toluene-4-sulfonic acid (3R, 3aS, 6R, 6aR)-6-hydroxy-hexahydro-furo[3,2-b]furan-3-yl ester in 30 ml of DMF is heated with 0.8 g of sodium azide under reflux for 2 hours, solvent is distilled off and the distillation residue obtained is dissolved in EtAc and extracted with H2O. The organic phase obtained is dried and solvent is evaporated. (3R, 3aR, 6S, 6aR)-6-azido-hexahydro-furo[3,2-b]furan-3-ol is obtained.
    • I-OC) {(3S, 3aR, 6R, 6aR)-6-Hydroxy-hexahydro-furo[3,2-b]furan-3-yl}-carbamic acid tert-butyl ester
  • To a solution of 1.5 g of (3R, 3aR, 6S, 6aR)-6-azido-hexahydro-furo[3,2-b]furan-3-ol in 25 ml of dioxane 75 mg of palladium on charcoal (10%) are added and the mixture obtained is subjected to hydrogenation. The mixture obtained is filtered and stirred overnight with 3.2 ml of ethyldiisopropylamine and 4.1 g of (BOC)2O. From the mixture obtained solvent is evaporated. The evaporation residue obtained is dissolved in EtAc and extracted with saturated aqueous sodium NaHCO3-solution, 1N HCl and brine. The organic phase obtained is dried, solvent is evaporated and the evaporation residue obtained is subjected to chromatography. {(3S, 3aR, 6R, 6aR)-6-hydroxy-hexahydro-furo[3,2-b]furan-3-y}-carbamic acid tert-butyl ester is obtained. 1HNMR(DMSO-d8): 7.1 (bs,1H,NH), 4.8 (d,1H,6-OH,J=10 Hz), 4.3 (dd,1H,H-6a,J=4.6 and 4.3 Hz), 4.27 (d,1H,H-3a,J=4.3 Hz), 4.0-4.1 (m,1H,H-6), 3.2-3.85 (m,5H,2xH-2, H-3, 2xH-5), 1.4 (s,9H,tert.butyl).
    • I-OD) Toluene-4-sulfonic acid (3R, 3aS, 6S, 6aR)-6-tert-butoxycarbonylamino-hexahydro-furo[3,2-b]furan-3-yl ester
  • A solution of 700 mg of {(3S, 3aR, 6R, 6aR)-6-hydroxy-hexahydro-furo[3,2-b]furan-3-yl}-carbamic acid tert-butyl ester in 10 ml of pyridine is stirred for 16 hours with 785 mg of toluolsulfonylchloride, solvent is distilled off and the distillation residue is dissolved in EtAc and extracted with 1N HCl, saturated aqueous NaHCO3-solution and H2O. The organic layer obtained is dried and solvent is evaporated. Toluene-4-sulfonic acid (3R, 3aS, 6S, 6aR)-6-tert-butoxycarbonylamino-hexahydro-furo[3,2-b]furan-3-yl ester is obtained.
    • I-OE) 14-O-{[(3S,3aS,6S,6aR)-6-tert-Butoxycarbonylamino-hexahydro-furo[3,2-b]furan-3-ylsulfanyl]-acetyl}-mutilin
  • 267 mg of potassium tert.butoxide are added to a solution of 950 mg of toluene-4-sulfonic acid (3R, 3aS, 6S, 6aR)-6-tert-butoxycarbonylamino-hexahydro-furo[3,2-b]furan-3-yl ester and 1032 mg of 14-mercapto-acetyl-mutilin in 20 ml of DMSO. The mixture obtained is stirred at 70° for 1 hour and distributed between EtAc and brine. The organic phase obtained is washed with H2O, dried and solvent is evaporated. The evaporation residue obtained is subjected to chromatography. 14-O-{(3S,3aS,6S,6aR)-6-tert-butoxycarbonyl-amino-hexahydro-furo[3,2-b]furan-3-ylsulfanyl]-acetyl}-mutilin is obtained. 1HNMR(DMSO-d6): 7.1 (bs,1H,NH), 6.1, 5.05, 5.0 (3xm,H19,H20,H21), 5.55 (d,1H,H14, J=8.2 Hz), 4.5 (m,2H,H11-OH,H-3a′), 4.4 (d, 1H, H-6a′, J=4 Hz), 3.3-4.0 (m,9H,H-2′,H-3′,H-5′,H-6′,H11,H22), 1.36 (s,9H,tert-butyl), 1.34, 1.05 (2xs, (CH3)15, (CH3)18), 0.8, 0.62 (2xd (CH3)16, (CH3)17,J=6.8 Hz).
    • I-OF) 14-O-{[(3S,3aS,6S,6aR)-6-Amino-hexahydro-furo[3,2-b]furan-3-ylsulfanyl]-acetyl}-mutilin
  • 950 mg of 14-O-{[(3S,3aS,6S,6aR)-6-tert-butoxycarbonylamino-hexahydro-furo[3,2-b]furan-3-ylsulfanyl]-acetyl}-mutilin are dissolved in 20 ml of CH2Cl2 and the mixture obtained is stirred for 2 hours with 3 ml of TFA. The mixture obtained is diluted with EtAc and extracted with saturated aqueous NaHCO3-solution. The organic phase obtained is dried and solvent is evaporated. 14-O-{[(3S,3aS,6S,6aR)-6-amino-hexahydro-furo[3,2-b]furan-3-ylsulfanyl]-acetyl}-mutilin is obtained.
    • I-OG) 14-O-{[(3S,3aS,6S,6aR)-6-Amino-hexahydro-furo[3,2-b]furan-3-ylsulfanyl]-acetyl}-mutilin in the form of a hydrochloride
  • 180 mg of 14-O-{[(3S,3aS,6S,6aR)-6-amino-hexahydro-furo[3,2-b]furan-3-ylsulfanyl]-acetyl}-mutilin are distributed between diethylether and 0.1N HCl. The aqueous layer obtained is lyophilized. 14-O-{[(3S,3aS,6S,6aR)-6-Amino-hexahydro-furo[3,2-b]furan-3-ylsulfanyl]-acetyl}-mutilin in the form of a hydrochloride is obtained. Characterisation data see in TABLE 4, Example 36a above.
    • Example I-P 14-O-{[(3S,3aS,6S,6aR)-6-((R)-Amino-3-methyl-butyrylamino)-hexahydro-furo[3,2-b]furan-3-ylsulfanyl]-acetyl}-mutilin in the form of a hydrochloride
  • A solution of 400 mg of 14-O-{[(3S,3aS,6S,6aR)-6-amino-hexahydro-furo[3,2-b]furan-3-ylsulfanyl]-acetyl}-mutilin in 20 ml of CH2Cl2 is treated with 128 mg of N-Boc-(R)-valine, 147 mg of EDC, 104 mg of HOBT and the mixture obtained is stirred overnight at RT. The mixture obtained is diluted with CH2Cl2, extracted with H2O, dried and solvent is evaporated. The evaporation residue obtained is subjected to chromatography on silica gel, relevant chromatographic fractions obtained are treated again with TFA in CH2Cl2, solvent is evaporated, the evaporation residue obtained is distributed between Et2O and 0.1N HCl and the aqueous layer obtained is lyophilized. 14-O-{[(3S,3aS,6S,6aR)-6-((R)-2-amino-3-methyl-butyrylamino)-hexahydro-furo[3,2-b]furan-3-ylsulfanyl]-acetyl}-mutilin in the form of a hydrochloride is obtained. Characterisation data see in TABLE 5, Example 38a.
    • Example I-Q 14-O-[((3-(R/S)-Amino-cyclohexan-1-(R/S)-yl)-sulfanyl acetyl)]-mutilin in the form of a hydrochloride
  • 10.6 g of pleuromutilin-22-O-tosylate dissolved in 10 ml of THF are slowly added to a solution of 5.2 g of N-BOC-3-(R/S)-mercapto-cyclohexylamin and 2.74 g of potassium-tert.butylate in 250 ml of THF. The mixture obtained is stirred for 2 hours, distributed between brine and EtAc, and extracted with 0.1N HCl. The organic phase obtained is dried, solvent is evaporated and the evaporation residue obtained is subjected o chromatography. 14-O-[((N-BOC-3(R/S)-amino-cyclohexan-1-(R/S)-yl)-sulfanyl acetyl)]-mutilin is obtained and is converted into 14-O-[((3-(R/S)-Amino-cyclohexan-1-(R/S)-yl)-sulfanyl acetyl)]-mutilin in the form of a hydrochloride by treatment with etheric HCl.
  • 1H-NMR(d6-DMSO): 8.0 (b,3H,NH3 +), 6.15, 5.1 (2xm,H19,H20,H21), 5.52(d,1H,J=5.2 Hz,H14). 3.4(m,1H,H11), 3.3 (m,2H,H22), 2.9 (m,1H,NCH), 2.7(m,1H,CHS), 1.18, 1.45 (2xs,(CH3)15, (CH3)18),0.75, 0.88 (2xd,(CH3)16,(CH3)17,J=5.4 Hz)
    • II. Preparation of Intermediates (Starting Materials) for the Preparation of a (Novel) Pleuromutilin of the Present Invention Example II-A 14-Mercapto-acetyl-mutilin II-AA) 14-O-[(Carbamimidoylsulfanyl)acetyl]mutilin-tosylate
  • A solution of 15.2 g of thiourea and 106.4 g of pleuromutilin-22-O-tosylate in 250 ml of acetone is heated under reflux for 1.5 hours, solvent is removed and 100 ml of hexane are added. A precipitate forms, is filtrated off and dried.
    • 14-O-[(carbamimidoylsulfanyl)acetyl]mutilin-tosylate is obtained II-AB) 14-Mercapto-acetyl-mutilin
  • A solution of 4.7 g of Na2S2O5 in 25 ml of H2O is added to a solution of 12.2 g of 14-O-[(carbamimidoylsulfanyl)acetyl]mutilin-tosylate in a mixture of 20 ml of EtOH and 35 ml of H2O (warmed to ca. 90°). 100 ml of CCl4 are added to the reaction mixture obtained and the mixture obtained is heated under reflux for ca. 2 hours. The two-phase system obtained is separated, the organic phase is dried and solvent is evaporated. 14-mercapto-acetyl-mutilin is obtained.
    • Example II-B N-BOC-3(R)-methylsulfonyloxy-piperidine II-BA) N-BOC-3(R)-Hydroxy-piperidine
  • A suspension of 3.48 g of 3-(R)-hydroxypiperidine, 8.72 g of di-tert.butyl-dicarbonat and 4 g of N-metyl-morpholine in 70 ml of dioxane is stirred for 18 hours at RT. From the mixture obtained solvent is evaporated and the evaporation residue obtained is dissolved in CH2Cl2 and extracted with 1N HCl. The organic phase obtained is dried and solvent is evaporated. N-BOC-3(R)-hydroxy-piperidine is obtained.
    • II-BB) N-BOC-3(R)-methylsulfonyloxy-piperidine
  • A solution of 5.08 g of N-BOC-3(R)-hydroxy-piperidine and 8.7 g of methanesulfonic acid anhydride in 100 ml pyridine is stirred at RT for 22 hours, pyridine is distilled off, the distillation residue is dissolved in CH2Cl2, the mixture obtained is extracted with 1N HCl, the organic phase obtained is dried, solvent is evaporated and the evaporation residue obtained is subjected to chromatography. N-BOC-3(R)-methylsulfonyloxy-piperidine is obtained. 1H-NMR(CDCl3): 4.7(m,1H,CHOSO2CH3), 3.2-3.6(m,4H,CHN), 3.0(s,3H,CH3SO2), 1.4(m, 9H,tert.butyl).
    • Example II-C N-BOC-Piperidine-3(S)-thiol II-CA) N-BOC-3-(S)-Thioacetoxy-piperidine
  • A solution of 2.2 g of N-BOC-3-(R)-hydroxy-piperidine in 10 ml of THF is added under argon and 1 ml of thiolacetic acid to a solution of 3.4 g of triphenylphosphine and 2.65 ml of azadicarbonic acid-isopropylate in 10 ml of THF. The mixture obtained is kept for 18 hours at 70°, solvent is evaporated and the evaporation residue obtained is subjected to chromatography. N-BOC-3-(S)-thioacetoxy-piperidine is obtained. 1H-NMR (CDCl3): 3.78 (dd,1H,NCH2CHS, J=3.1 Hz, J=13.3 Hz), 3.5-3.6 (m,2H, CHSC—O, NCH2CH2), 2.32(s,3H,SC═OCH3), 1.46(s,9H, tert.butyl)
    • II-CB) N-BOC-Piperidine-3(S)-thiol
  • To a solution of 259 mg of N-BOC-3-(S)-thioacetoxy-piperidine in 10 ml of MeOH a solution of 262 mg of NaSCH3 in 5 ml of MeOH is added and the mixture obtained is stirred for 2 hours, solvent is evaporated and the evaporation residue obtained is distributed between EtAc and aqueous HCl. Solvent from the organic phase obtained is evaporated. N-BOC-piperidine-3(S)-thiol is obtained. 1H-NMR(d6-DMSO): 2.6 (d,1H,SH, J=7.2 Hz), 2.9-2.7 (m,3H, NCH2,CHS), 1.35 (b,9H,tert.butyl). MS(ESI) 457 (2M+Na).
    • Example II-D 3-(N-BOC-(R)-Valyl-amino)-cyclohexane-(R/S)-thiol II-DA) 3-(R/S)-(2,4,6-Trimethyl-benzyl-sulfanyl)-cyclohexanone
  • A solution of 3.32 g of 2,4,6-trimethyl-benzylmercaptane and 3.84 g of cyclohexen-3-one in 30 ml of pyridine is heated at 400 for 3 hours. The mixture obtained is poured into 200 ml of 1M HCl and the mixture obtained is extracted with CH2Cl2. The organic phase obtained is dried, solvent is evaporated and the evaporation residue is subjected to chromatography on silica gel. 3-(R/S)-(2,4,6-trimethyl-benzyl-sulfanyl)-cyclohexanone is obtained. 1H-NMR(d6-DMSO): 6.8 (s,2H,arom.H), 3.8 (s,2H,C6H5CH2S—), 3.3 (m,1H,CHS), 3.18 (dd,1H, CHC═NOH, J=4 Hz,13.9 Hz), 2.65-2.8, 2.44-2.49(2xm,4H,CH2C═OCH2), 2.15, 2.3(9H,3xCH3).
    • II-DB) 3-(R/S)-(2,4,6-Trimethyl-benzyl-sulfanyl)-cyclohexanone-oxime (syn and anti-forms)
  • A solution of 5.24 g of 3-(R/S)-(2,4,6-trimethyl-benzyl-sulfanyl)cyclohexanone, 1.38 g of hydroxylamine in the form of a hydrochloride and 2 g of triethylamine in 50 ml of MeOH is stirred at 25° for 12 hours, the mixture obtained is poured into 200 ml of brine and the mixture obtained is extracted with CH2Cl2. The organic phase obtained is dried and solvent is evaporated. A mixture of the syn- and anti forms of 3-(R/S)-(2,4,6-trimethyl-benzyl-sulfanyl)-cyclohexanone-oxime is obtained which is subjected to chromatography. Pure syn- and pure anti-3-(R/S)-(2,4,6-trimethyl-benzyl-sulfanyl)-cyclohexanone-oxime is obtained.
  • 1H-NMR(d6-DMSO) of the syn-form: 10.3 (s,1H,OH), 6.8 (s,2H,arom.H), 3.75 (s,2H, C6H5CH2S—), 2.88 (m,1H,CHS), 3.18 (dd,1H,CHC═NOH, J=4 Hz,13.9 Hz), 2.13 (dd,1H, CHC═NOH, J=5.2 Hz,13.9 Hz), 2.15, 2.3(9H, 3xCH3).
  • 1H-NMR(d6-DMSO) of the anti-form: 10.3 (s,1H,OH), 6.8 (s,2H,arom.H), 3.75 (s,2H, C6H5CH2S—), 2.92(m,1H,CHS), 2.58 (dd,1H,CHC═NOH, J=4 Hz,13.9 Hz), 2.15 (dd,1H, CHC═NOH, J=4.2 Hz,13.6 Hz), 2.15, 2.3(9H, 3xCH3).
    • II-DC) 3-(R/S)-(2,4,6-trimethyl-benzyl-sulfanyl)-cyclohexyl-(R/S)-amine
  • 2.7 g of 3-(R/S)-(2,4,6-Trimethyl-benzyl-sulfanyl)-cyclohexanone-oxime are added to a mixture of 20 ml of a 1M-solution of LiAlH3 and 15 ml of dioxane, the mixture obtained is heated for 1 hour at 80° and the mixture obtained is poured into 200 ml of a 20% aqueous NH4Cl-solution. The mixture obtained is extracted with EtAc, the organic phase obtained is dried and solvent is evaporated. 3-(R/S)-(2,4,6-trimethyl-benzyl-sulfanyl)-cyclohexyl-(R/S)-amine is obtained.
    • II-DC) 3-(N-BOC-(R)-Valyl-amino)-cyclohexan-1-(R/S)-yl-sulfanylmethyl-(2,4,6-trimethyl-benzol)
  • A mixture of 1.05 g of 3-(R/S)-(2,4,6-trimethyl-benzyl-sulfanyl)-cyclohexyl-(R/S)-amine, 870 mg of BOC-R-valine, 760 mg of EDC and 404 mg of triethylamine in 20 ml THF is stirred at RT for 2 hours. The mixture obtained is distributed between brine and EtAc, the organic phase obtained is dried, solvent is evaporated and the evaporation residue is subjected to chromatography. 3-(N-BOC-(R)-valyl-amino)-cyclohexan-1-(R/S)-yl-sulfanylmethyl-(2,4,6-trimethyl-benzol) is obtained. 1H-NMR(d6-DMSO): Rotamers, 7.78, 7.3, 6.52 (3xd,2H,NH), J=7.9 Hz), 6.8, 6.82 (2xs,2H, arom.H), 6.55 (m,1H,NHC═O),3.7 (m,1H,a-H-valyl), 3.6 (m,1H,NHCH), 2.75, 3.0 (2xm,1H, CHS), 1.39(s,9H,tert.butyl)
    • II-DD) 3-(N-BOC-(R)-Valyl-amino)-cyclohexane-(R/S)-thiol
  • 10 ml ammonia are condensed at −70° within a solution of 600 mg of 3-(N-BOC-(R)-valyl-amino)-cyclohexan-1-(R/S)-yl-sulfanylmethyl-2,4,6-trimethyl-benzol in 15 ml of THF and sodium is added in portions until the solution remains deep blue. Solid NH4Cl is added to the mixture obtained and the mixture obtained is allowed to warm up to RT, is flushed with nitrogen, the solid residue obtained is filtered off, the filtrate obtained is concentrated and subjected to chromatography on silica gel. 3-(N-BOC-(R)-Valyl-amino)-cyclohexane-(R/S)-thiol is obtained. 1H-NMR(d6-DMSO): Rotamer, 7.75 (m,1H,NHCHC═O), 6.55 (m,1H,NHC═O), 2.75 (m,1H,CHS), 2.58(d,1H,SH,J=6.6 Hz), 1.39(s,9H,tert.butyl)
  • Analogously to the method as set out in Example II-D but using appropriate starting materials the following compounds are obtained:
    • Example 11-D-1 N-BOC-3-(R/S)-mercapto-cyclohexylamin Example II-D-2 4-(R/S)-Mercapto-azepan-2-one
  • from 4-(R/S)-(2,4,6-trimethyl-benzyl-sulfanyl)-azepan-2-one. 1H-NMR(d6-DMSO): 6.15, 5.1 (2xm,H19,H20,H21), 5.52(d,1H,J=5.2 Hz,H14), 3.4(m,1H,H11), 3.3 (m,2H,H22), 2.99-3.12(b,2H,CH2N), 3.18(m,1H,SCH), 2.7 (m,1H,C═OCH), 2.67(d,1H,SH,J=5.5 Hz), 2.58(d,1H,C═OCH,J=13.5 Hz), 1.18, 1.45 (2xs,(CH3)15,(CH3)18), 0.75, 0.88 (2xd,(CH3)16,(CH3)17,J=5.4 Hz).
    • Example II-E N-BOC-3-(R/S)-(2,4,6-trimethyl-benzyl-sulfanyl)-cyclohexyl-(R/S)-amine
  • A solution of 11 g of 3-(R/S)-(2,4,6-trimethyl-benzyl-sulfanyl)-cyclohexylamine, 9.15 g of BOC-anhydride and 4.2 g of triehylamine in 100 ml of THF is stirred at 25° for 12 hours, solvent is evaporated and the concentrated residue is distributed between CH2Cl2 and 1M HCl. From the organic phase obtained solvent is evaporated and the evaporation residue is subjected to chromatography. N-BOC-3-(R/S)-(2,4,6-trimethyl-benzyl-sulfanyl) cyclohexyl-(R/S)-amine is obtained. 1H-NMR(d6-DMSO): 6.81, (s,1H,NHCO), 6.8 (s,2H, arom.H), 3.75 (s,2H,C6H5CH2S—), 3.2 (m,1H,CHN), 2.70 (m,1H,CHS), 2.13 2.15, 2.3(9H, 3xCH3), 1.4(s,9H,tert.butyl)
    • Example II-F Toluene-4-sulfonic acid (3S, 3aS, 6R, 6aR)-6-hydroxy-hexahydro-furo[3,2-b]furan-3-yl ester and Toluene-4-sulfonic acid (3R, 3aS, 6S, 6aR)-6-hydroxy-hexahydro-furo[3,2-b]furan-3-yl ester
  • A solution of 8.76 g of (38, 3aS, 6R, 6aR)-hexahydro-furo[3,2-b]furan-3,6-diol in 80 ml of pyridine is stirred for 16 hours with 13.7 g of toluenesulfonylchloride, solvent is distilled off and the distillation residue is dissolved in EtAc and extracted with 1N HCl, saturated aqueous NaHCO3-solution and H2O. The organic layer obtained is dried, solvent is evaporated and the evaporation residue is subjected to chromatography. Toluene-4-sulfonic acid (3S, 3aS, 6R, 6aR)-6-hydroxy-hexahydro-furo[3,2-b]furan-3-yl ester (a) and toluene-4-sulfonic acid (3R, 3aS, 6S, 6aR)-6-hydroxy-hexahydro-furo[3,2-b]furan-3-yl ester (b) are obtained. 1HNMR(DMSO-d6) of form (a): 7.8 (d,2H,Ar—H,J=8.2 Hz), 7.5 (d,2H,Ar—H,J=8.6 Hz), 4.95 (d,1H,6-OH), 4.8 (m, 1H, H-3), 4.42 (dd,1H,H-6a,J=4.6 and 4.8 Hz), 4.38 (d,1H,H-3a,J=4.6), 4.08 (m,1H,H-6), 3.8 (m,2H,2×H-2), 3.7, 3.25 (2×dd, 2H, 2×H-5), 2.4 (s, 3H, Ar—CH3). 1HNMR(DMSO-d6) of form (b): 7.8 (d,2H,Ar—H,J=8.6 Hz), 7.5 (d,2H,Ar—H,J=8.6 Hz), 5.15 (d, 6-OH, J=3.5), 4.9 (m,1H,H-3), 4.45 (dd,1H,H-3a,J=4.3 and 4.8 Hz), 4.2 (d,1H,H-6a,J=4.3), 4.0 (m,1H,H-6), 3.7 (m,3H,H-2 and 2xH-5), 3.5 (dd,1H,H-2,J=9.5 and 6.3 Hz), 2.4 (s, 3H, Ar—CH3).
    • Example II-G N-BOC-4-Hydroxy-piperidin-3-thiol, N-BOC-3-Hydroxy-piperidin-4-thiol
  • A solution of 1 g of N-BOC-3,4-epoxy-piperidin, 1.9 g of triphenylsilylmercaptane and 0.7 ml of triethylamine in 12.5 ml of THF is stirred for 24 hours at 70°, and 1.7 g of TBAF and 0.9 ml acetic acid are added. The mixture obtained is stirred for 1 hour and distributed between brine and EtAc. The organic phase is dried, solvent is evaporated and the evaporation residue obtained is subjected to chromatography on silica gel. (a): N-BOC-4-Hydroxy-piperidin-3-thiol, and (b): N-BOC-3-hydroxy-piperidin-4-thiol are obtained.
  • 1H-NMR (CDCl3) of (a): 4.45, 4.12, 2.8 (3xm,3H,CH2NCH), 3.31 (dt, 1H, CHO, J=4.3 Hz,J=10 Hz), 2.65, 2.6(2xm,2H,CHN,CHS), 1.5(s,9H,tert.butyl).
  • 1H-NMR (CDCl3) of (b): 4.25, 3.45 2.7, (3xm,3H,CH2NCH), 3.2 (m,1H,CHO), 2.55(m,2H, NCH,CHS), 1.5(s,9H,tert.butyl).
    • TEST EXAMPLES Example A
  • Determination of Mycobacterium tuberculosis Strain Resistance
  • Activity of the known compounds Isoniacid, Rifampicin and Streptomycin against M. tuberculosis strains 1 to 14 as set out in TABLE A is determined in the Agar Dilution Test according to the method as described in the General Procedure. The MIC is determined after 3, 4 and 5 weeks. The strains 1 to 7 tested were found to be either sensible (S) or resistant (R) against Isoniacid, Rifampicin and/or Streptomycin. Results are as set out in TABLE A below:
    TABLE A
    M. tuberculosis Isoniacid Rifampicin Streptomycin
    Strain 1 S S S
    Strain 2 S S S
    Strain 3 R R S
    Strain 4 R R R
    Strain 5 R R S
    Strain 6 S S S
    Strain 7 R S R
    Strain 8 S R S
    Strain 9 R S R
    Strain 10 S S S
    Strain 11 S S S
    Strain 12 S S S
    Strain 13 S S S
    Strain 14 S S S
    Strain 15 S S S

    Strain 15 is the labor strain 137 kV. Resistant and sensible strains are isolated from patients with known sensibilities/resistance. A strain is designated as resistant if its MIC in testing according to the General Test Procedure after 3 to 5 weeks is higher than 20 μg/ml.
    • Example B
  • Activity of TEST COMPOUNDS (TCs) against M. tuberculosis strains 1 to 5 and 7 as set out in TABLE A Is determined in the Agar Dilution Test under conditions as in Example A in different agar concentrations of the TEST COMPOUNDS. The MIC is determined after 3, 4 and 5 weeks.
  • Activity of the following TEST COMPOUNDS (TC) is tested:
    A compound of formula I-Tiamulin: TC-1
    A compound of formula I-Valnemulin: TC-2
    A compound of formula I-PREF1: TC-3
    A compound of formula I-PREF2: TC-4
    A compound of formula I-PREF3: TC-5
    A compound of formula I-PREF4: TC-6
    A compound of formula I-PREF5: TC-7
    A compound of formula I-PREF6: TC-8
    A compound of formula I-PREF7: TC-9
    A compound of formula I-PREF8: TC-10
  • Test results are as set out in TABLE B below are obtained:
    TABLE B
    MIC (μg/ml) against Mycobacterium
    tuberculosis of strain number
    TC/week 1 2 3 4 6 7
    TC-1/3 5 5 5 5 10 10
    TC-1/4 5 5 5 10 10 20
    TC-1/5 5 5 5 10 10 20
    TC-2/3 0.5 1 1 5 5 5
    TC-2/4 0.5 5 1 5 5 5
    TC-2/5 0.5 5 5 5 5 10
    TC-3/3 1 5 5 1 5 20
    TC-3/4 1 5 5 5 10 20
    TC-3/5 1 5 5 10 10 20
    TC-4/3 0.5 5 1 1 5 5
    TC-4/4 1 5 1 5 5 5
    TC-4/5 1 5 5 5 5 5
    TC-5/3 1 5 5 5 5 5
    TC-5/4 5 5 5 5 10 10
    TC-5/5 5 5 5 10 10 10
    • Example C
  • Is carried out according to the method of Example B. Test results obtained are as set out in TABLE C and in TABLE D and in TABLE E:
    TABLE C
    MIC (μg/ml) against Mycobacterium
    tuberculosis of strain number
    TC/week 1 2 3 4 5 6 7 8
    TC-2/3 0.5 4 2 8 2 4 4 4
    TC-2/4*) 0.5 4 2 8 4 4 8 8
    TC-4/3 1 4 1 4 2 4 2 2
    TC-4/4 2 4 1 4 2 4 4 2
    TC-4/5 2 4 1 4 2 4 4 4

    *)Tested after 31 days and thus no further testing after 5 weeks.
  • TABLE D
    MIC (μg/ml) against Mycobacterium
    tuberculosis of strain number
    TC/week 10 11 12 13 14 15
    TC-2/3 4 2 4 4 4 2
    TC-2/4*) 4 4 4 8 4 4
    TC-4/3 2 2 2 2 4 2
    TC-4/4 2 2 2 2 4 2
    TC-4/5 4 2 4 4 4 4

    *)Tested after 31 days and thus no further testing after 5 weeks.
  • TABLE E
    MIC (μg/ml) against
    Mycobacterium tuberculosis
    of strain number
    TC/week 1 12 15
    TC-6/2 1 1 0.5
    TC-7/1 2 32 32
    TC-8/1 2 32 32
    TC-9/1 8 32 32
    TC-10/2 2 16 4
  • In TABLES B to E in the column “TC/week” the term “TC-number” indicates the TEST COMPOUND as defined above, e.g. TO-1 indicates a compound of formula I—Tiamulin; and “/week” indicates the MIC-determination point (in weeks from inoculation) in the testing of such TEST COMPOUND. “TC-1/3” for example indicates that the MIC of a compound of formula I—Tiamulin was determined after 3 weeks from inoculation.
  • MIC is the minimum inhibition concentration as defined above. The strain numbers 1 to 4 and 6 to 7 indicated In TABLE B, the strain numbers 1 to 9 indicated in TABLE C and the strain numbers 10 to 15 indicated in TABLE D, and the strain numbers 1, 12 and 15 indicated in TABEL E refer to the corresponding Mycobacterium tuberculosis strains of
    TABLE 6
    REX REX REX
    Compound IEX
    Figure US20050215637A1-20050929-C00111
    Figure US20050215637A1-20050929-C00112
    Figure US20050215637A1-20050929-C00113
    Compound IEX
    Figure US20050215637A1-20050929-C00114
    Figure US20050215637A1-20050929-C00115
    Figure US20050215637A1-20050929-C00116
    Compound IEX
    Figure US20050215637A1-20050929-C00117
    Figure US20050215637A1-20050929-C00118
    Figure US20050215637A1-20050929-C00119
    Compound IEX
    Figure US20050215637A1-20050929-C00120
    Figure US20050215637A1-20050929-C00121
    Compound I′EX
    Figure US20050215637A1-20050929-C00122

Claims (18)

1. Use of a pleuromutilin in the preparation of a medicament for the treatment of diseases caused by Mycobacterium.
2. A method of treating diseases mediated by Mycobacterium, comprising administering to a subject in need of such treatment an effective amount of a pleuromutilin.
3. A method according to claim 2 wherein Mycobacterium is Mycobacterium tuberculosis.
4. A method according to claim 2 wherein a pleuromutilin is a compound comprising the basic structural elements as set out in formula
Figure US20050215637A1-20050929-C00123
wherein R is vinyl or ethyl and the dotted line is a bond or is no bond.
5. Use or a method according to claim 4 wherein a pleuromutilin is selected from the group consisting of
a compound of formula
Figure US20050215637A1-20050929-C00124
a compound of formula,
Figure US20050215637A1-20050929-C00125
a compound of formula
Figure US20050215637A1-20050929-C00126
a compound of formula
Figure US20050215637A1-20050929-C00127
a compound of formula
Figure US20050215637A1-20050929-C00128
a compound of formula
Figure US20050215637A1-20050929-C00129
a compound of formula
Figure US20050215637A1-20050929-C00130
a compound of formula
Figure US20050215637A1-20050929-C00131
a compound of formula
Figure US20050215637A1-20050929-C00132
and
a compound of
Figure US20050215637A1-20050929-C00133
6. A compound selected from the group consisting of compounds of formulae as set out in TABLE 1:
TABLE 1
Figure US20050215637A1-20050929-C00134
Figure US20050215637A1-20050929-C00135
Figure US20050215637A1-20050929-C00136
Figure US20050215637A1-20050929-C00137
Figure US20050215637A1-20050929-C00138
Figure US20050215637A1-20050929-C00139
Figure US20050215637A1-20050929-C00140
Figure US20050215637A1-20050929-C00141
Figure US20050215637A1-20050929-C00142
Figure US20050215637A1-20050929-C00143
Figure US20050215637A1-20050929-C00144
Figure US20050215637A1-20050929-C00145
Figure US20050215637A1-20050929-C00146
Figure US20050215637A1-20050929-C00147
Figure US20050215637A1-20050929-C00148
7. A compound of formula
Figure US20050215637A1-20050929-C00149
wherein
the dotted line is a bond (double bond between positions a=b), R1A is hydrogen and R2A is not present, or
the dotted line is no bond (single bond between positions a-b) and R1A and R2A independently of each other are hydrogen, halogen or deuterium,
R3A is (C1-6)alkyl,
R4A is hydrogen, (C1-6)alkyl, a group —C(═NH)—NH2, or the residue of an amino acid,
R5A is hydrogen, or
R4A and R5A together are a group ═CH—NH2,
R6A is hydrogen or deuterium, and
mA is 0, 1, 2, 3, 4, or 5.
8. A 14-O-(oximino-(C3-8)cycloalkyl-sulfanylmethylcarbonyl)-pleuromutilin and a 14-O-(hydrazono-(C3-8)cycloalkyl-sulfanylmethylcarbonyl)-pleuromutilin.
9. A compound of formula
Figure US20050215637A1-20050929-C00150
wherein
R1B has the meaning of R1A as defined in claim 7,
R2B has the meaning of R2A as defined in claim 7,
R10B has the meaning of R6A as defined in claim 7,
the dotted line has the meaning as defined in claim 7,
mB has the meaning of ma as defined above,
R3B is hydrogen or (C1-6)alkyl,
XB is —O—R4B or —NR5BR6B,
R4B is hydrogen or (C1-6)alkyl, optionally substituted by a group —NR7BR8B,
R5B and R6B independently of each other are (C1-4)alkyl,
R7B and R8B independently of each other are (C1-4)alkyl, or
R7B and R8B together with the nitrogen atom to which they are attached form aliphatic heterocyclyl, having 5 to 8 ring members, and
R9B is hydrogen or (C1-4)alkyl.
10. A compound of formula
Figure US20050215637A1-20050929-C00151
wherein
R1C has the meaning of R1A as defined in claim 7,
R2C has the meaning of R2A as defined in claim 7,
the dotted line has the meaning as defined in claim 7,
R4C has the meaning of R6A as defined in claim 7, and
R3C is amino, (C1-4)alkylamino, di(C1-4)alkylamino, the residue of an amino acid, hydroxy, or (C1-4)alkoxy.
11. A compound of formula
Figure US20050215637A1-20050929-C00152
wherein
R1D has the meaning of R1A as defined in claim 7,
R2D has the meaning of R2A as defined in claim 7,
R4D has the meaning of R6A as defined in claim 7,
the dotted line has the meaning as defined in claim 7, and
R3D is aliphatic heterocyclyl of 4 to 8 ring members, and comprising one nitrogen atom as the heteroatom, or (C4-8)cycloalkyl, which heterocyclyl or cycloalkyl is substituted by hydroxy or oxo.
12. A compound of claim 7 in the form of a salt.
13. A compound of claim 7 for use as a pharmaceutical.
14. A pharmaceutical composition comprising a compound of claim 7 in association with at least one pharmaceutical excipient.
15. A pharmaceutical composition according to claim 13, further comprising another pharmaceutically active agent.
16. A pharmaceutical composition comprising a pleuromutilin in association with at least one pharmaceutical excipient, and further comprising another pharmaceutically active agent useful in the treatment of Mycobacterium infections.
17. A compound of claim 7 for use in the preparation of a medicament for the treatment of microbial diseases.
18. A method of treatment of microbial diseases, which method comprises administering to a subject in need of such treatment an effective amount of a compound of claim 7.
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